Pre-clinical Models for Studying the Interaction Between Mesenchymal Stromal Cells and Cancer Cells and the Induction of Stemness

Conclusions Several model systems are now available to characterize the MSC-tumour interplay in the TME. These offer early promise in establishing robust preclinical platforms for the identification of crucial molecular pathways and for the assessment of clinical efficacy of novel drugs to inhibit cancer development and progression. However, selection of the right model for a given study should be shaped on the purpose, and should also consider fixed biological, biochemical, and biophysical parameters according to the specific tumour type. Finally, in order to get reliable and useful results to be translated to the clinic, it should be always kept in mind the careful comparisons in the prediction of human outcomes by the developed model. Author Contributions All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication. Funding This study was funded by the Italian Association for Cancer Research (AIRC IG# 21403 to NB) and by the Ministry of Health 5x1000 fundings (to NB at Rizzoli Orthopaedic Institute). Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. References 1. Ronnov-Jessen L, Petersen OW, Bissell MJ. Cellular changes involved in conversion of normal to malignant breast: importance of the stromal reaction. Physiol Rev. (1996) 76:69–125. ...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research

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In this study, we found that TLR1/TLR2 expression predicted favorable prognosis of lung cancer patients. In the related mice tumor model, TLR1/TLR2 activation by synthetic bacterial lipoprotein (BLP), a TLR1/2 agonist, greatly inhibited tumor growth and selectively decreased monocytic MDSCs (M-MDSCs). Furthermore, BLP treatment redirected M-MDSC differentiation towards M1 macrophage through JNK pathway, and thus blocked the suppressive activity of M-MDSCs in a TLR2-dependent manner. Therefore, our data demonstrated that TLR2 could be a promising biomarker and a potential immunotherapeutic target for lung cancer.
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 14 June 2019Source: Stem Cell ResearchAuthor(s): Nupur Bhargava, Shashank Jaitly, Sangam Giri Goswami, Suman Jain, Debojyoti Chakraborty, Sivaprakash RamalingamAbstractSickle cell disease (SCD) is an autosomal recessive disorder caused by a mutation in β-globin (HBB) gene. We have generated an induced pluripotent stem cell (iPSC) line, IGIBi001-A from an Indian sickle cell patient with a homozygous HBB gene mutation using Sendai virus reprogramming system. Characterization of IGIBi001-A showed that these iPSCs are transgene-free and expressed pluripotent stem cell markers. They had a...
Source: Stem Cell Research - Category: Stem Cells Source Type: research
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Source: Stem Cell Research - Category: Stem Cells Source Type: research
This report could be used to provide guidance for both practical and economical approaches. PMID: 31190617 [PubMed - as supplied by publisher]
Source: Acta Cardiologica - Category: Cardiology Tags: Acta Cardiol Source Type: research
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Source: Physica E: Low dimensional Systems and Nanostructures - Category: Nanotechnology Source Type: research
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Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research
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Source: Nano Today - Category: Nanotechnology Source Type: research
In this study, we report a mild synthesis of sub-10 nm Cu nanoparticles (NPs) in ethylene glycol (EG) at room temperature using l-ascorbic acid (AA) as the reducing agent in the presence of 1-amino-2-propanol (AMIP). To the best of our knowledge, this work is the first report of the room-temperature synthesis of sub-10 nm Cu NPs using a nontoxic reducing agent. The as-prepared colloidal suspension of Cu NPs exhibited surface plasmon resonance absorption at 515 nm. Transmission electron microscopy observations revealed well-dispersed spherical Cu NPs with an average particle size of 4.1 ± 0.8 nm. The use ...
Source: Colloids and Interface Science Communications - Category: Nanotechnology Source Type: research
This study shows the possibility of simultaneous culturing of human OB and OC precursors and demonstrates the efficacy of dopants in the pace change of bone remodeling for bone tissue engineering applications.Graphical abstract
Source: Materials Today Communications - Category: Materials Science Source Type: research
Publication date: 2019Source: Materials Today: Proceedings, Volume 15, Part 2Author(s): Sumit Das Lala, Payel Deb, Emon Barua, Ashish.B. Deoghare, Sushovan ChatterjeeAbstractNano-sized Hydroxyapatite (HAp) powders are synthesized from natural bio-waste chicken eggshell. The synthesized powder is characterized using X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) which confirms the formation of HAp. Scanning Electron Microscopy (SEM) shows highly porous agglomerated HAp powder with average pore diameter of 276.3 nm. EDAX analysis reveals calcium and phosphate ratio (Ca/P) of 1.74 showing close res...
Source: Materials Today: Proceedings - Category: Materials Science Source Type: research
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