Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry

Discussion Tibroviruses are highly undercharacterized rhabdoviruses with unknown pathogenic potential. All isolated tibroviruses (BAV, BHV, CPV, SWBV, and TIBV) have only been found in biting midge vectors or in various, apparently healthy non-human mammals (Cybinski et al., 1980; Standfast et al., 1984; Cybinski and Gard, 1986; Gibbs et al., 1989). The discovery of BASV, EKV-1, and EKV-2 genomes in human sera (Grard et al., 2012; Stremlau et al., 2015) suggests that at least some tibroviruses could infect humans. Because BASV was discovered in the serum of a severely ill individual, tibroviruses should be considered potential human pathogens for surveillance purposes until this hypothesis is ruled out. In this initial study, we aimed to comprehensively characterize the host and cell type tropism of tibroviruses to determine whether tibroviruses in general can enter human and other animal cells. Previous studies on this topic have been extremely limited. BAV and SWBV were isolated in Asian tiger mosquito (C6/36) cells, and all attempts to grow them in baby golden hamster (Mesocricetus auratus) kidney (BHK-21) and adult grivet (C. aethiops) kidney (Vero) cells failed (Gibbs et al., 1989). BHV was isolated only in porcine stable-equine kidney (PS-EK) cells (Standfast et al., 1984) and in C6/36 cells after a single Vero cell passage (Huang et al., 2016). CPV was isolated in BHK-21, C6/36 cells, and Vero cells (Cybinski and Gard, 1986). TIBV was originally isolated in suckling ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research