Fight Aging! Newsletter, February 27th 2023
This study tested the hypothesis that ischemic vascular repair in aging by Ang-(1-7) involves attenuation of myelopoietic potential in the bone marrow and decreased mobilization of inflammatory cells.
Young or Old male mice of age 3-4 and 22-24 months, respectively, received Ang-(1-7) for four weeks. Myelopoiesis was evaluated in the bone marrow (BM) cells by carrying out the colony forming unit (CFU-GM) assay followed by flow cytometry of monocyte-macrophages. Expression of pro-myelopoietic factors and alarmins in the hematopoietic progenitor-enriched BM cells was evaluated. Hindlimb ischemia (HLI) was induced by femoral ligation, and mobilization of monocytes into the blood stream was determined. Blood flow recovery was monitored and infiltration of inflammatory cells was evaluated by immunohistochemistry.
BM cells from Old mice generated a higher number of monocytes and M1 macrophages compared to that of Young, which was reversed by Ang-(1-7). Gene expression of selected myelopoietic factors, alarmins, and the receptor for alarmins, RAGE, was higher in the Old hematopoietic progenitor-enriched BM cells compared to the Young. Increased expressions of these factors were decreased by Ang-(1-7). Ischemia-induced mobilization of monocytes was higher in Old mice with decreased blood flow recovery and increased infiltration of monocyte-macrophages compared to the Young, all of which were reversed by Ang-(1-7). Enhanced ischemic vascular repair by Ang-(1-7) in aging...
Source: Fight Aging! - Category: Research Authors: Reason Tags: Newsletters Source Type: blogs
More News: Academia | Allergy & Immunology | Alzheimer's | Biochemistry | Biology | Biotechnology | Blindness | Brain | Brain Cancers | Cancer | Cancer & Oncology | Carbohydrates | Cardiology | Cardiovascular | Chemistry | Child Development | Children | Chronic Kidney Disease | Clinical Trials | Diabetes | Diabetes Type 2 | Dialysis | Diets | Disability | Eating Disorders & Weight Management | Education | Electronic Health Records (EHR) | Endocrinology | Environmental Health | Epidemiology | Fruit | Funding | Gastroschisis Repair | Genetics | Government | Heart | Heart Disease | Heart Transplant | Hypertension | Immunotherapy | Infectious Diseases | Insulin | Iron | Israel Health | Kidney Transplant | Kidney Transplantation | Laboratory Medicine | Lawsuits | Learning | Legislation | Lithuania Health | Men | Metabolic Syndrome | Microdeletion Syndromes | Mitochondria | Mitochondrial Disease | Molecular Biology | Neurology | Neuroscience | Nurses | Nursing | Nutrition | Obesity | Opthalmology | Orthopaedics | Pathology | Research | Science | Sodium Chloride | Sports Medicine | Statistics | Stem Cell Therapy | Stem Cells | Study | Sugar | Toxicology | Transplant Surgery | Transplants | Universities & Medical Training | Urology & Nephrology | Women