Loss-of-Function in SMAD4 Might Not Be Critical for Human Natural Killer Cell Responsiveness to TGF- β
This study was carried out in accordance with approval of the Melbourne Health and Walter and Eliza Hall Institute of Medical Research's Human Research Ethics Committee (approval number: 2013.081). All subjects gave written informed consent for participation and publication. Results and Discussion TGF-β signaling in NK cells is associated with: phosphorylation in SMAD2 and 3, inhibition of IL-15-induced metabolism/proliferation, simultaneous downregulation of CD44, CD49e, and Eomes, and upregulation of CD16 and CD49a expression (7, 10). SMAD family member 4 (SMAD4) belongs to the SMAD family of transcription factor proteins which combine in heterocomplexes with SMAD2 and 3, and still have an unclear role during the signal transduction (11, 12). In this case report, we obtained samples from patients carrying a loss-of-function mutation in the SMAD4 (p.Ser232GInfs*3) expected to generate a protein without function and prone to be degraded upon expression. We accessed by western blot the SMAD4 protein expression, from fresh isolated NK cell by negative selection, two SMAD4-mutants and 2 health donors, and confirmed a lower expression of SMAD4 in both patient samples (data not shown). SMAD4-mutant and healthy donor NK cell phenotypes were assessed by gating the CD56bright and CD56dim subsets from the viable CD3neg, CD14neg, CD20neg, CD45+, CD66bneg, NKp46+ PBMC population. Although all donors were clinically classified as infection-free at time of donation, SMAD4-m...
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A missed diagnosis of COVID-19 in this 94-year-old who presented with delirium, low-grade fever, and abdominal pain resulted in spread among healthcare workers and other patients.Age and Ageing
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Authors: Polistina GE, Simioli F, Imitazione P, Lanza M, Annunziata A, Fiorentino G Abstract The coronavirus disease 2019 (COVID-19) is a recent pandemic that affected more than 5 million people worldwide. Chest high resolution computed tomography (HRCT) is an essential tool in diagnosis and management of the disease. Pulmonary parenchymal opacity is a typical sign of the disease, but not the only one. Pneumothorax, pneumomediastinum, bronchiectasis and cysts are probably underrated complications of COVID-19 that can worsen prognosis, in terms of prolonged hospitalization and need of oxygen therapy. In our single c...
Publication date: Available online 20 September 2020Source: Biochimica et Biophysica Acta (BBA) - Reviews on CancerAuthor(s): Marjolein M.G. Kes, Jan Van den Bossche, Arjan W. Griffioen, Elisabeth J.M. Huijbers
Publication date: Available online 19 September 2020Source: Biochimica et Biophysica Acta (BBA) - Reviews on CancerAuthor(s): Hang-Ping Yao, Rachel Hudson, Ming-Hai Wang
Conclusion: The results of this review indicate that most osteopathic medical schools are at the early stages of EPA implementation, with emphasis varying by program year in terms of the specific EPAs addressed. Many schools appear engaged in curricular change efforts that will support the advancement of EPA use within their institutions. Faculty development was identified as a continued critical need for a majority of institutions. PMID: 32946547 [PubMed - as supplied by publisher]
Conclusion: Moderate quality evidence suggests that OMTh is effective for CNCP patients. There was a significant association between visceral OMTh and reduced pain severity and disability. More robust, high-quality randomized controlled trials with larger sample sizes are required to further explore the effectiveness of the OMTh in the management of CNCP. PMID: 32946545 [PubMed - as supplied by publisher]
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