Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support

This reporter group also includes lines driven by ubiquitous promoters like CMV and human ubiquitin C. These are particularly useful for cut-and-paste, transplantation-based experiments to label and fate map regions of host embryos. The second group of reporter expression lines includes transgenics marking subcellular organelles (Supplementary Table S1B). These are highly useful for the study of molecular processes involved in cell function and are especially effective when utilized in the context of Xenopus egg extracts, the only cell-free system that permits full investigation of all DNA transactions related to cell cycle progression and DNA damage repair (Cross and Powers, 2009; Hoogenboom et al., 2017). Many of these lines have been generated by the Ueno lab and mark a diverse range of organelles including plasma membrane, Xla.Tg (CMV:RFP-CAAX)Ueno (RRID:EXRC_0075, NXR_0115), golgi bodies, Xla.Tg (CMV:Has.B4GALT1-eGFP)Ueno (RRID:EXRC_0077, NXR_0110), and microtubule plus ends, Xla.Tg (CMV:Has.MAPRE3-eGFP)Ueno (RRID:EXRC_0078, NXR_0109) (Takagi et al., 2013). Lines generated by other labs, as well as by the stock centers themselves, that label other organelles are also available. The third group of reporter lines are those that serve as indicators of signaling activity (Supplementary Table S1C). These include Wnt signaling reporter lines generated by the Vleminckx lab in both X. laevis and X. tropicalis (Tran et al., 2010), an apoptosis sensor (Kominami et al., 2006), a ...
Source: Frontiers in Physiology - Category: Physiology Source Type: research