Effect of Compound Kushen Injection, a Natural Compound Mixture, and Its Identified Chemical Components on Migration and Invasion of Colon, Brain, and Breast Cancer Cell Lines

Effect of Compound Kushen Injection, a Natural Compound Mixture, and Its Identified Chemical Components on Migration and Invasion of Colon, Brain, and Breast Cancer Cell Lines Saeed Nourmohammadi1†, Thazin Nwe Aung2†, Jian Cui2, Jinxin V. Pei1, Michael Lucio De Ieso1, Yuka Harata-Lee2, Zhipeng Qu2, David L. Adelson2* and Andrea J. Yool1* 1Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia 2Department of Molecular and Biomedical Science, School of Biological Sciences, The University of Adelaide, Adelaide, SA, Australia Traditional Chinese Medicines are promising sources of new agents for controlling cancer metastasis. Compound Kushen Injection (CKI), prepared from medicinal plants Sophora flavescens and Heterosmilax chinensis, disrupts cell cycle and induces apoptosis in breast cancer; however, effects on migration and invasion remained unknown. CKI, fractionated mixtures, and isolated components were tested in migration assays with colon (HT-29, SW-480, DLD-1), brain (U87-MG, U251-MG), and breast (MDA-MB-231) cancer cell lines. Human embryonic kidney (HEK-293) and human foreskin fibroblast (HFF) served as non-cancerous controls. Wound closure, transwell invasion, and live cell imaging showed CKI reduced motility in all eight lines. Fractionation and reconstitution of CKI demonstrated combinations of compounds were required for activity. Live cell imaging confirmed CKI strongly reduced migration of HT-29 and MDA-MB-231 cells, m...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research

Related Links:

The confidence of intimate partner violence (IPV) survivors/victims in the criminal justice system (CJS) is important to consider when exploring intervention and prevention strategies toward deterring IPV. Information on the experiences of IPV survivors/vi...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Jurisprudence, Laws, Legislation, Policies, Rules Source Type: news
Extensive research has dealt with violence directed at healthcare workers in hospital settings; however, few studies have examined community-based settings. Furthermore, there is also scant literature regarding the perceptions of healthcare providers who w...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Occupational Issues Source Type: news
This study aimed to validate the Chinese version of the WEB scale u...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Young Adults Source Type: news
The phenomenon of Sexual Assault against Women in Late Life (SAWLL) has received increasing research attention in recent years. Yet, there is still only scant knowledge on it, compared with research on the sexual assault of younger women. Sexual assault is...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Alcohol and Other Drugs Source Type: news
Publication date: Available online 7 July 2020Source: The American Journal of SurgeryAuthor(s): Kurt S. Schultz, Susanna W.L. de Geus, Teviah E. Sachs, Ryan B. Morgan, Sing Chau Ng, David McAneny, Jennifer F. Tseng
Source: The American Journal of Surgery - Category: Surgery Source Type: research
The objectives of this paper are the presentation of a sample of patients with PC undergoing treatment with androgen deprivation therapy (ADT) in usual clinical practice and the determination of parameters associated with the development of resistance to castration (CRPC). MATERIAL AND METHODS: Multicenter, observational, retrospective study that analyzes patients treated with ADT from January 2016 to January 2017. Descriptive analysis of the most relevant clinical variables and univariante analysis and progression times by Kaplan-Meier test. RESULTS: Sample of 952 patients. At PC diagnosis median age 74 years. Med...
Source: Archivos Espanoles de Urologia - Category: Urology & Nephrology Tags: Arch Esp Urol Source Type: research
CONCLUSIONS: We concluded that despite the fact that the plasmacytoid variant of BC is rare, it is important to take into account the pathologic and clinical features of this tumor in order to manage the optimal treatment of this poor prognosis cancer. PMID: 32633253 [PubMed - in process]
Source: Archivos Espanoles de Urologia - Category: Urology & Nephrology Tags: Arch Esp Urol Source Type: research
Patient age, breast density, and radiation exposure are among the strongest,...Read more on AuntMinnie.comRelated Reading: Earlier breast screening benefits women with childhood cancer Interval breast cancers have unique characteristics Breast imagers can help identify women for genetic testing Women want breast cancer risk info from radiologists Many women at risk for breast cancer don't take action
Source: AuntMinnie.com Headlines - Category: Radiology Source Type: news
CONCLUSION: To address flaws in conceptualisation and the resulting gaps in knowledge, we suggest a definition and a theoretical framework that are suited to allow heterogeneity in the field, but enables the development of sound interventions, as well as facilitate the interpretation of intervention effectiveness. PMID: 32631110 [PubMed - in process]
Source: European Journal of General Practice - Category: Primary Care Tags: Eur J Gen Pract Source Type: research
CBSN political reporter Caitlin Huey-Burns and Washington Post politics reporter Eugene Scott join CBSN's "Red&Blue" to discuss  the White House response to the coronavirus pandemic, plans for the Republican National Convention, and Joe Biden's presidential campaign.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
More News: Academia | Adenocarcinoma | Alopecia | Alternative and Complementary Therapies | Australia Health | Biology | Biomedical Science | Biotechnology | Brain | Brain Cancers | Breast Cancer | Breast Carcinoma | Calcium | Canada Health | Cancer | Cancer & Oncology | Cancer Therapy | Carcinoma | Chemistry | Chemotherapy | China Health | Chloride | Colon Cancer | Colorectal Cancer | Complementary Medicine | Drugs & Pharmacology | Eloxatin | Endocrinology | Epithelial Cancer | Fiji Health | Flavonoids | Gastric (Stomach) Cancer | Gastroenterology | Genetics | Germany Health | Grants | Harvard | Hepatocellular Carcinoma | Herbs | Hormones | Infertility | Japan Health | Legislation | Lessons | Leukemia | Liver | Liver Cancer | Lung Cancer | Macau Health | Molecular Biology | Neurology | Neuroscience | New Zealand Health | Non-Small Cell Lung Cancer | Oral Cancer | Organic | Palliative | Palliative Care | Pancreas | Pancreatic Cancer | PET Scan | Rectal Cancers | Reproduction Medicine | Science | Skin Cancer | Squamous Cell Carcinoma | Statistics | Study | Toxicology | Training | Universities & Medical Training | Urology & Nephrology | USA Health