Fight Aging! Newsletter, July 3rd 2023

In this study, cultured adipose-derived stem cells (ASCs) were derived from subcutaneous white adipose tissue isolated from mice fed a normal diet. We performed senescence-associated-β-galactosidase (SA-β-gal) staining, real-time PCR, and Western blot to evaluate the levels related to cellular senescence markers. The mRNA expression levels of senescence markers were significantly increased in the later passages of ASCs. We show that light activation reduced the expression of senescent genes, and SA-β-Gal in all cells at passages. Moreover, the light-activated ASCs-derived exosomes decrease the expression of senescence, and SA-β-Gal in the later passage cells. We further investigated the photoreceptive effect of Opsin3 (Opn3) in light-activated ASCs. Deletion of Opn3 abolished the differences of light activation in reduced expression of senescent genes, increased Ca2+ influx, and cAMP levels. We explored the effects of photomodulation on exosome secretion. The concentration of light-treated ASC exosomes represented approximately a fivefold increase compared with non-light-treated ASCs. Light-activated ASC-derived exosomes could represent a new protective paradigm for cellular senescence resulting from in-vitro passaging. How to Run a Comparatively Simple Self-Experiment to Assess the Impact of Taurine Supplementation on Measures of Aging https://www.fightaging.org/archives/2023/06/how-to-run-a-comparatively-simple-self-experiment-to-assess-the-...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs