Zeolites as Carriers of Antitumor Ribonuclease Binase

In this study we tested the clinoptilolite, chabazite, and natrolite ability to be loaded by antitumor ribonuclease binase and the cytotoxicity of the obtained complexes. We found the optimal conditions for binase loading into zeolites and established the dynamic of its release. Cytotoxic effects of zeolite-binase complexes toward colorectal cancer Caco2 cells were characterized after 24 and 48 h of incubation with cells using MTT-test. Zeolites were toxic by itselfs and reduced cells viability by 30% (clinoptilolite), 40% (chabazite), and 70% (natrolite) after 48 h of incubation. Binase complexes with clinoptilolite as well as chabazite always demonstrated enhanced toxicity (up to 57 and 60% for clinoptilolite and chabazite, respectively) in comparison with binase and zeolites separately. Our results contribute to the perspective development of binase-based complexes for therapy of colorectal cancer for or the treatment of malignant skin neoplasms where the complexes can be used in pasty form. Introduction There are about 40 naturally occurring tectosilicate minerals in zeolite group, the most commonly mined isometric forms include chabazite and clinoptilolite, the fibrous form is mainly represented by natrolite. Chemical differentiation of zeolites is related to the ratio of SiO2/Al2O3 and water content. Zeolites with an Al/Si ratio of 0.20–0.40 are leafy, others with an Al/Si ratio up to 0.50 are isometric or mostly isometric, and with an Al/Si ratio of 0.60&...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research

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In this study, 5% w/v SMP dispersion was acidified to pH 2.4–3.0 using citric acid and heated at different conditions. Lowering pH resulted in dissolution of colloidal calcium phosphate from casein micelles, corresponding to lowered turbidity and reduced hydrodynamic diameter of dispersions. When heated for 10 min at different temperatures, 70 °C was the most effective to produce transparent dispersions; whereas a shorter heating time between 2 and 60 min was desired at 90 °C. The decreased turbidity after heating was attributed to weakened hydrophobic interaction between casein molecules based on f...
Source: Food Hydrocolloids - Category: Food Science Source Type: research
Publication date: Available online 14 October 2019Source: Food ChemistryAuthor(s): Jianghao Sun, Craig S. Charron, Janet A. Novotny, Bing Peng, Liangli Yu, Pei ChenAbstractBroccoli is a popular brassica vegetable and its consumption may decrease the occurrence of cancer in certain populations. To gain insight into the metabolites that may induce physiological responses to broccoli intake, a non-targeted metabolomic approach and a targeted approach for analysis of glucosinolate metabolites were developed using high resolution accurate mass spectrometry. A human study was conducted in which 6 subjects consumed a single meal ...
Source: Food Chemistry - Category: Food Science Source Type: research
In this study, we developed a methodology combined Paternò-Büchi reaction nanoelectrospray ionization mass spectrometry (PB-nanoESI-MS) and nuclear magnetic resonance (NMR) to identify C=C locations and isomers of PUTAGs in sacha inchi oil. Benzophenone was used as the PB reagent, and the optimized solvent composition (methanol:chloroform=9:1) allowed for PUTAGs and their PB products to be detected with higher intensities. In addition, we made efforts to interpret the MS2 spectra for identification lipid species. A series of C57-PUTAGs and C59-PUTAGs were detected and identified via high-resolution PB-nanoESI-M...
Source: Food Chemistry - Category: Food Science Source Type: research
Publication date: Available online 14 October 2019Source: Food ChemistryAuthor(s): Tayse Ferreira Ferreira da Silveira, Letícia Maeda Cajaíba, Leonardo Valentin, Bruno Barea, Pierre Villeneuve, Inar Alves CastroAbstractOil-in-water (O/W) emulsions are important delivery systems of omega-3 fatty acids (n-3 FA). We investigated the effect of sinapic acid esters concentration and chain length, the electrical charge of the emulsifier and emulsion pH on the oxidative stability of n-3 FA rich O/W emulsions. Echium oil was applied as n-3 FA source. A 24 factorial design was used to simultaneously evaluate these fact...
Source: Food Chemistry - Category: Food Science Source Type: research
This study may provide useful details about the biomedical applications of CeO2 NPs.
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
Publication date: 15 December 2019Source: Journal of Molecular Liquids, Volume 296Author(s): Xu Hu, Xi Zhao, Yangchuan KeAbstractSynthesis of a novel β-cyclodextrin-functionalized hydrophobically associating polyacrylamide named ASM was carried out using acrylamide (AM), cetyl dimethyl allyl ammonium bromide (SD-16) and modified β-cyclodextrin (M-β-CD) via free-radical copolymerization. Effective characterization methods, including FT-IR, 1H NMR and TGA, were adopted to evaluate ASM. Influences of SiO2 nanoparticle (nano-SiO2) on ASM solution properties were evaluated. The results showed that the addition of...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
Publication date: Available online 14 October 2019Source: Journal of Molecular LiquidsAuthor(s): Pei-Hsuan Tung, Bin Laiwang, Chi-Min Shu, Kuang-Hua HsuehAbstractModern refined lubricants have several advantages over their predecessors. However, numerous modern lubricants are environmentally unfriendly, and professional technicians must dispose of lubricant by-products at recycling plants. The primary problem of lubricant by-products is their susceptibility to accidental fire. Both environmental pollution and lubricant-related fire hazards affect the lives of people who live and work near lubricant recycling plants. To exa...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
Publication date: Available online 13 October 2019Source: Redox BiologyAuthor(s): Bingtian Xu, Yunyun Qin, Dan Li, Ningbo Cai, Jinling Wu, Lan Jiang, Limei Jie, Zhongzhen Zhou, Jiangping Xu, Haitao WangAbstractInhibition of phosphodiesterase 4 (PDE4) produces neuroprotective effects against cerebral ischemia. However, the involved mechanism remains unclear. Augmentation of endoplasmic reticulum (ER) stress promotes neuronal apoptosis, and excessive oxidative stress is an inducer of ER stress. The present study aimed to determine whether suppression of ER stress is involved in the protective effects of PDE4 inhibition again...
Source: Redox Biology - Category: Biology Source Type: research
Publication date: Available online 14 October 2019Source: Fish &Shellfish ImmunologyAuthor(s): Jiajun Xie, Haohang Fang, Shiyu Liao, Tianyu Guo, Peng yin, Yongjian Liu, Lixia Tian, Jin NiuAbstractA feeding experiment was conducted to determine the effects of Schizochytrium sp. on growth performance, antioxidant capacity and non-specific immunity in golden pompano (Trachinotus ovatus).Two diets were formulated with or without Schizochytrium sp. supplemented (D1:0% and D2: 3%) to feed fish for 8 weeks. Results showed that growth performance, feed intake and survival rate increased significantly with Schizochytrium sp. supplemented (P 
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Publication date: Available online 13 October 2019Source: Advances in Biological RegulationAuthor(s): Veronica Balatti, Carlo M. CroceAbstractWe established that loss of miR-15a/16–1 genes on chromosome 13q14 is the most common alteration in Chronic Lymphocytic Leukemia (CLL) and that miR-15/16 are crucial negative regulator of BCL-2, an antiapoptotic gene overexpressed in most CLLs and in many other malignancies. We have also shown that miR-15/16 target ROR1, a cell surface receptor for Wnt5a which can enhance growth/survival of CLL cells. Interestingly, ROR1 is expressed by many cancers, but not by normal adult tis...
Source: Advances in Biological Regulation - Category: Biology Source Type: research
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