Engineered Extracellular Vesicles From Human Periodontal-Ligament Stem Cells Increase VEGF/VEGFR2 Expression During Bone Regeneration

Engineered Extracellular Vesicles From Human Periodontal-Ligament Stem Cells Increase VEGF/VEGFR2 Expression During Bone Regeneration Jacopo Pizzicannella1†, Agnese Gugliandolo2†, Tiziana Orsini3, Antonella Fontana4, Alessia Ventrella4, Emanuela Mazzon2*, Placido Bramanti2, Francesca Diomede1‡ and Oriana Trubiani1‡ 1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti–Pescara, Chieti, Italy 2IRCCS Centro Neurolesi “Bonino Pulejo,” Messina, Italy 3Institute of Cell Biology and Neurobiology, National Research Council, Rome, Italy 4Department of Pharmacy, “G. d’Annunzio” University of Chieti–Pescara, Chieti, Italy Bone regeneration represents still a challenge, in particular for calvarium defects. Recently, the development of biomaterials with the addiction of stem cells is giving promising results for the treatment of bone defects. In particular, it was demonstrated that scaffolds enriched with mesenchymal stem cells (MSCs) and/or their derivatives, such as conditioned medium (CM) and extracellular vesicles (EVs), may improve bone regeneration. Moreover, given the deep link between osteogenesis and angiogenesis, a successful approach must also take into consideration the development of vascularization. In this work we evaluated the bone regeneration capacity of a collagen membrane (3D-COL) enriched with human periodontal-ligament stem ce...
Source: Frontiers in Physiology - Category: Physiology Source Type: research

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Authors: Xiao W, Hu C, Chu C, Man Y Abstract This prospective pilot study presents a novel method using the dentin shell technique for the three-dimensional reconstruction of alveolar bone defects. Autogenous dentin shell (≤ 2 mm) harvested from impacted third molars was fixed at the recipient site as an external barrier. The space between the dentin shell and residual bone was filled with deproteinized bovine bone mineral particles mixed with concentrated growth factor (CGF) pieces. Autogenous bone (gold standard for bone graft) was applied to prepare shell grafts as the control. A total of 13 patients were inc...
Source: International Journal of Periodontics and Restorative Dentistry - Category: Dentistry Tags: Int J Periodontics Restorative Dent Source Type: research
This study aimed to compare the bone regeneration capacities of MinerOss X, Bio-Oss, and autograft in rabbit calvaria. In this animal study, four defects measuring 8 mm in diameter were created in the calvaria of 10 rabbits using a trephine bur. Three defects were randomly filled with MinerOss X, Bio-Oss, and autogenous bone. The fourth defect remained empty as a control. The rabbits were sacrificed after 4 or 8 weeks. Calvarial samples were taken and histopathologically evaluated. The mean number of osteoblasts and the amount of newly formed bone in all groups were higher at 8 compared to 4 weeks. At 4 and 8 weeks, the am...
Source: International Journal of Periodontics and Restorative Dentistry - Category: Dentistry Tags: Int J Periodontics Restorative Dent Source Type: research
Authors: Adawi H, Hengjeerajaras P, Froum SJ, Bagheri Z Abstract The purpose of this article is to compare the larger (8 × 10-mm single implant and 15 × 20-mm multiple implant) lateral window designs used in the sinus elevation procedure with a newly proposed, less-invasive window design and discuss the advantages and limitations of the new design. The less-invasive window designs differ from the larger window designs in both the size and shape of lateral windows. Following creation of a round-window osteotomy, the quality of the sinus membrane and its ability to be elevated are assessed. The initial wi...
Source: International Journal of Periodontics and Restorative Dentistry - Category: Dentistry Tags: Int J Periodontics Restorative Dent Source Type: research
Authors: Fistarol F, De Stavola L, Fincato A, Bressan E Abstract The aim of this study is to evaluate the anatomical characteristics of the posterior region of the mandible and their surgical relevance related to bone harvesting procedures. Fifty retromolar cone beam computed tomography scans were analyzed considering the donor site anatomies. For each site, linear measurements were taken of cross-sectional scans to record perpendicular distances between the mandibular canal (MC) and the vestibular and crestal bone walls. Data showed that the distance from the MC to the vestibular bone wall is lower in the ramus ar...
Source: International Journal of Periodontics and Restorative Dentistry - Category: Dentistry Tags: Int J Periodontics Restorative Dent Source Type: research
The objective of this study was to compare the novel extraction-site development (XSD) technique with spontaneous healing. Advanced alveolar defects (extraction defect sounding, classes 3 and 4) at 33 single-rooted teeth were treated by XSD (test), and 21 extraction sites of single-rooted teeth were left for spontaneous healing (control). In pre- and postoperative cone beam computed tomography (CBCT) data sets, orovestibular and vertical socket dimensions were assessed, as were socket areas. XSD resulted in complication-free healing with significantly higher radiographic bone fill compared to spontaneous healing. Applicati...
Source: International Journal of Periodontics and Restorative Dentistry - Category: Dentistry Tags: Int J Periodontics Restorative Dent Source Type: research
Authors: Wessels R, Eghbali A, De Roose S, De Bruyckere T, Vervaeke S, Cosyn J Abstract Partial-thickness double pedicle flap and connective tissue graft (CTG) is a procedure to treat single gingival recession in the anterior mandible. However, long-term data have not been reported. Patients who had been treated by the same periodontist in a private practice in Belgium between 2002 and 2009 were invited to return for a clinical examination in 2017. Seventeen patients (15 females, 2 males; mean age: 34.6 years) with a total of 23 treated single recessions were reexamined after 8 to 15 years of follow-up. Between 1 a...
Source: International Journal of Periodontics and Restorative Dentistry - Category: Dentistry Tags: Int J Periodontics Restorative Dent Source Type: research
This article outlines the basic physics of electricity, in particular the principles behind diathermy, the hazards posed by it and by other devices and the various measures available to reduce the risk of these.
Source: Anaesthesia and intensive care medicine - Category: Anesthesiology Source Type: research
Publication date: Available online 15 October 2019Source: Anaesthesia &Intensive Care MedicineAuthor(s): Kevin Wu, Fin O'SullivanAbstractPoisoning with chemical agents was once thought to be confined to the battlefield. However, over the past decade there has been an increase in the use of chemical weapon agents and toxic industrial chemicals as weapons of terror. As well as use during conflict, these poisons have been used in other attacks with deadly effects. These agents require particular treatments that fall out with standard medical practice to reduce harm and prevent contamination of medical treatment facilities...
Source: Anaesthesia and intensive care medicine - Category: Anesthesiology Source Type: research
This article will provide an update on the most recent evidence base on clinical practice, including the use of acute severe respiratory failure bundles and extracorporeal techniques to support lung protective ventilation.
Source: Anaesthesia and intensive care medicine - Category: Anesthesiology Source Type: research
Publication date: Available online 15 October 2019Source: Anaesthesia &Intensive Care MedicineAuthor(s): Emma L. Hartley, Stuart GillonAbstractIn the last 20 years there have been significant advances in extracorporeal support of the respiratory system. What once was a highly complex intervention, undertaken as a salvage procedure in a handful of patients, has become more wide spread, both in terms of availability and underlying indications. We review the principles of equipment; physiological control of oxygenation and decarboxylation; associated complications; and role in clinical practice. The evidence for extracorp...
Source: Anaesthesia and intensive care medicine - Category: Anesthesiology Source Type: research
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