Log in to search using one of your social media accounts:

 

Translocation Renal Cell Carcinoma: An Update
Microphthalmia-associated transcription (MiT) family translocation renal cell carcinoma (tRCC) comprises Xp11 tRCC and t(6;11) RCC.08/29/2017 (Source: Kidney Cancer Association)
Source: Kidney Cancer Association - August 29, 2017 Category: Urology & Nephrology Source Type: news

Scientists map sex chromosome evolution in pathogenic fungi
(Duke University) Duke researchers recently mapped the evolutionary turning point that transformed the pathogenic Cryptococcus fungus from an organism with thousands of sexes to only two. They found that during evolution, a reshuffling of DNA known as translocation brought together separate chunks of sex-determining genes onto a single chromosome, essentially mimicking the human X or Y chromosome. Surprisingly, these translocations occurred at the chromosome's centromeres, regions so dense that they were once thought to suppress recombination. (Source: EurekAlert! - Infectious and Emerging Diseases)
Source: EurekAlert! - Infectious and Emerging Diseases - August 11, 2017 Category: Infectious Diseases Source Type: news

Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104
Hsp100 polypeptide translocases are conserved members of the AAA+ family (adenosine triphosphatases associated with diverse cellular activities) that maintain proteostasis by unfolding aberrant and toxic proteins for refolding or proteolytic degradation. The Hsp104 disaggregase from Saccharomyces cerevisiae solubilizes stress-induced amorphous aggregates and amyloids. The structural basis for substrate recognition and translocation is unknown. Using a model substrate (casein), we report cryo–electron microscopy structures at near-atomic resolution of Hsp104 in different translocation states. Substrate interactions ar...
Source: ScienceNOW - July 20, 2017 Category: Science Authors: Gates, S. N., Yokom, A. L., Lin, J., Jackrel, M. E., Rizo, A. N., Kendsersky, N. M., Buell, C. E., Sweeny, E. A., Mack, K. L., Chuang, E., Torrente, M. P., Su, M., Shorter, J., Southworth, D. R. Tags: Biochemistry r-articles Source Type: news

Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading
Bidirectional replication from eukaryotic DNA replication origins requires the loading of two ring-shaped minichromosome maintenance (MCM) helicases around DNA in opposite orientations. MCM loading is orchestrated by binding of the origin recognition complex (ORC) to DNA, but how ORC coordinates symmetrical MCM loading is unclear. We used natural budding yeast DNA replication origins and synthetic DNA sequences to show that efficient MCM loading requires binding of two ORC molecules to two ORC binding sites. The relative orientation of these sites, but not the distance between them, was found to be critical for MCM loading...
Source: ScienceNOW - July 20, 2017 Category: Science Authors: Coster, G., Diffley, J. F. X. Tags: Biochemistry, Molecular Biology reports Source Type: news

'Overturning established fact' leads to new new target in MLL-rearranged leukemia
(University of Colorado Anschutz Medical Campus) A University of Colorado Cancer Center paper published today in the journal Cancer Cell challenges existing understanding of potential therapeutic targets in MLL-translocation leukemia. Specifically, the study shows that within the family of MLL-related proteins, MLL2 and not MLL is the most appropriate target for drugs challenging the disease. In other words, drug developers aiming at MLL may have been missing slightly to one side of the real target. (Source: EurekAlert! - Medicine and Health)
Source: EurekAlert! - Medicine and Health - June 13, 2017 Category: International Medicine & Public Health Source Type: news

Cytogenetic Abnormalities Do Not Affect Prognosis in Pediatric CML
Chromosomal abnormalities such as a variant t(9;22) translocation do not appear to have significant prognostic impact on children with chronic myeloid leukemia. (Source: CancerNetwork)
Source: CancerNetwork - May 22, 2017 Category: Cancer & Oncology Authors: Dave Levitan Tags: Chronic Myeloid Leukemia Hematologic Malignancies News Source Type: news

Tetraspanin microdomains control localized protein kinase C signaling in B cells
Activation of B cells by the binding of antigens to the B cell receptor (BCR) requires the protein kinase C (PKC) family member PKCβ. Because PKCs must translocate to the plasma membrane to become activated, we investigated the mechanisms regulating their spatial distribution in mouse and human B cells. Through live-cell imaging, we showed that BCR-stimulated production of the second messenger diacylglycerol (DAG) resulted in the translocation of PKCβ from the cytosol to plasma membrane regions containing the tetraspanin protein CD53. CD53 was specifically enriched at sites of BCR signaling, suggesting that BCR-d...
Source: Signal Transduction Knowledge Environment - May 9, 2017 Category: Science Authors: Zuidscherwoude, M., Dunlock, V.-M. E., van den Bogaart, G., van Deventer, S. J., van der Schaaf, A., van Oostrum, J., Goedhart, J., In 't Hout, J., Hämmerling, G. J., Tanaka, S., Nadler, A., Schultz, C., Wright, M. D., Adjobo-Hermans, M. J. W., va Tags: STKE Research Articles Source Type: news

Research questions effectiveness of translocation conservation method
A DNA study of endangered greater prairie chickens in Illinois indicates that supplementing the dwindling population with birds from out of state did not improve genetic diversity. (Source: ScienceDaily Headlines)
Source: ScienceDaily Headlines - March 22, 2017 Category: Science Source Type: news

[Research Article] Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking
An inflammation-associated kinase also stimulates glucose uptake through plasma membrane translocation of GLUT4 in response to insulin. (Source: Signal Transduction Knowledge Environment)
Source: Signal Transduction Knowledge Environment - March 21, 2017 Category: Science Authors: Maeran Uhm, Merlijn Bazuine, Peng Zhao, Shian-Huey Chiang, Tingting Xiong, Sheelarani Karunanithi, Louise Chang, Alan R. Saltiel Source Type: news

Abnormal development of the brain in an intractable disease, thanatophoric dysplasia
In this study, by combining ferrets, whose brain is rather similar to that of humans, and unique technique developed by the present researchers, neuronal translocation along radial glial fibers to the cerebral cortex during fetal brain development has been discovered to be aberrant, which suggests the cause underlying TD. (Source: ScienceDaily Headlines)
Source: ScienceDaily Headlines - March 17, 2017 Category: Science Source Type: news

Abnormal development of the brain in an intractable disease, thanatophoric dysplasia
In this study, by combining ferrets, whose brain is rather similar to that of humans, and unique technique developed by the present researchers, neuronal translocation along radial glial fibers to the cerebral cortex during fetal brain development has been discovered to be aberrant, which suggests the cause underlying TD. (Source: EurekAlert! - Medicine and Health)
Source: EurekAlert! - Medicine and Health - March 17, 2017 Category: Global & Universal Source Type: news

[Perspective] Hematopoietic stem cells gone rogue
Cardiovascular disease is considered to be an aging-related disease and is the leading cause of death in the elderly in developed countries (1). As of 2013, 65% of deaths attributed to cardiovascular disease occurred among patients 75 years and older. A hallmark of aging is the accumulation of somatic DNA mutations in proliferative tissue. Although somatic mutations in the hematopoietic (blood cell) system are frequently observed in patients with hematological cancers, there is also a close correlation between hematopoietic somatic mutations and increased incidence of diabetes, atherosclerosis, and cardiovascular disease-r...
Source: ScienceNOW - February 23, 2017 Category: Science Authors: Yanfang Peipei Zhu Tags: Cardiovascular Disease Source Type: news

Targeted sequencing of FKBP5 in suicide attempters with bipolar disorder - Breen ME, Gaynor SC, Monson ET, de Klerk K, Parsons MG, Braun TA, DeLuca AP, Zandi PP, Potash JB, Willour VL.
FKBP5 is a critical component of the Hypothalamic-Pituitary-Adrenal (HPA) axis, a system which regulates our response to stress. It forms part of a complex of chaperones, which inhibits binding of cortisol and glucocorticoid receptor translocation to the n... (Source: SafetyLit)
Source: SafetyLit - January 5, 2017 Category: Global & Universal Tags: Ergonomics, Human Factors, Anthropometrics, Physiology Source Type: news

[Research Article] The receptor tyrosine kinase AXL mediates nuclear translocation of the epidermal growth factor receptor
The kinase AXL promotes cetuximab resistance by promoting the nuclear accumulation of EGFR. (Source: Signal Transduction Knowledge Environment)
Source: Signal Transduction Knowledge Environment - January 3, 2017 Category: Science Authors: Toni M. Brand, Mari Iida, Kelsey L. Corrigan, Cara M. Braverman, John P. Coan, Bailey G. Flanigan, Andrew P. Stein, Ravi Salgia, Jana Rolff, Randall J. Kimple, Deric L. Wheeler Source Type: news

[Editors' Choice] NIK sends mitochondria to the periphery
The kinase NIK promotes tumor cell invasion by stimulating the translocation of mitochondria to the cell periphery. (Source: Signal Transduction Knowledge Environment)
Source: Signal Transduction Knowledge Environment - January 3, 2017 Category: Science Authors: Nancy Gough (mailto:ngough at aaas.org) Source Type: news

[Perspective] Spying on the neighbors' pool
The structure and properties of the proton in water are of fundamental importance in many areas of chemistry and biology. The high mobility of the proton in an aqueous solution is understood in terms of its “hopping” between neighboring water molecules, as suggested by the two-century-old Grotthuss mechanism. The barrier for this process intimately depends on the proton's surrounding environment, which is manifested by the connectivity of the immediate hydrogen-bonding network as well as its dynamics caused by thermal fluctuations. On page 1131 of this issue, Wolke et al. (1) shed new light on the role that the...
Source: ScienceNOW - December 1, 2016 Category: Science Authors: Sotiris S. Xantheas Tags: Chemical Physics Source Type: news

[Research Article] A nuclease that mediates cell death induced by DNA damage and poly(ADP-ribose) polymerase-1
Inhibition or genetic deletion of poly(ADP-ribose) (PAR) polymerase-1 (PARP-1) is protective against toxic insults in many organ systems. The molecular mechanisms underlying PARP-1–dependent cell death involve release of mitochondrial apoptosis-inducing factor (AIF) and its translocation to the nucleus, which results in chromatinolysis. We identified macrophage migration inhibitory factor (MIF) as a PARP-1–dependent AIF-associated nuclease (PAAN). AIF was required for recruitment of MIF to the nucleus, where MIF cleaves genomic DNA into large fragments. Depletion of MIF, disruption of the AIF-MIF interaction, o...
Source: ScienceNOW - October 6, 2016 Category: Science Authors: Yingfei Wang Source Type: news

Critical But Overlooked: ICU Patients' Gut Bacteria
Paul Wischmeyer Our bodies are full of bacteria, and when we get sick, those microbial populations change. Hospitals monitor patients' bloodwork and vitals, so why not track the makeup of their microbiomes too? Paul Wischmeyer and his collaborators are conducting research that could allow them to do just that, opening the door for microbiome diagnostic indicators and probiotic measures to restore patients' normal bacterial signatures. We asked him about the research, and what he's learned so far. This text was edited for length. Read the full interview on ResearchGate News. ResearchGate: How did you first get involve...
Source: Science - The Huffington Post - September 6, 2016 Category: Science Source Type: news

OriGene's ALK UltraMAB® Antibody (OTI1A4) Outperform D5F3...
Recent Studies Validate High performance and Accurate Results for OriGene's UltraMAB® OTI1A4 as an Effective Screening Tool for ALK Translocation in Lung Adenocarcinomas(PRWeb April 14, 2016)Read the full story at http://www.prweb.com/releases/2016/04/prweb13344220.htm (Source: PRWeb: Medical Pharmaceuticals)
Source: PRWeb: Medical Pharmaceuticals - April 15, 2016 Category: Pharmaceuticals Source Type: news

[Report] Activation of proto-oncogenes by disruption of chromosome neighborhoods
Oncogenes are activated through well-known chromosomal alterations such as gene fusion, translocation, and focal amplification. In light of recent evidence that the control of key genes depends on chromosome structures called insulated neighborhoods, we investigated whether proto-oncogenes occur within these structures and whether oncogene activation can occur via disruption of insulated neighborhood boundaries in cancer cells. We mapped insulated neighborhoods in T cell acute lymphoblastic leukemia (T-ALL) and found that tumor cell genomes contain recurrent microdeletions that eliminate the boundary sites of insulated nei...
Source: ScienceNOW - March 24, 2016 Category: Science Authors: Denes Hnisz Source Type: news

Bacterial DNA a Potential Biomarker of Crohn's RelapseBacterial DNA a Potential Biomarker of Crohn's Relapse
In patients with Crohn's disease in remission, bacterial DNA (bactDNA) translocation may be a biomarker that can identify who is more likely to flare, according to new research. Reuters Health Information (Source: Medscape Pathology Headlines)
Source: Medscape Pathology Headlines - March 21, 2016 Category: Pathology Tags: Gastroenterology News Source Type: news

Marine Corps postpones plans to translocate 1,185 tortoises for training grounds
The Marine Corps base at Twenty-Nine Palms said Friday it had postponed plans to move 1,185 tortoises off prospective combat training grounds until wildlife agencies can determine whether the plan complies with the Endangered Species Act. The $50-million tortoise translocation effort was expected... (Source: Los Angeles Times - Science)
Source: Los Angeles Times - Science - March 19, 2016 Category: Science Authors: Louis Sahagun Source Type: news

[Report] Prostaglandin E2 constrains systemic inflammation through an innate lymphoid cell–IL-22 axis
Systemic inflammation, which results from the massive release of proinflammatory molecules into the circulatory system, is a major risk factor for severe illness, but the precise mechanisms underlying its control are not fully understood. We observed that prostaglandin E2 (PGE2), through its receptor EP4, is down-regulated in human systemic inflammatory disease. Mice with reduced PGE2 synthesis develop systemic inflammation, associated with translocation of gut bacteria, which can be prevented by treatment with EP4 agonists. Mechanistically, we demonstrate that PGE2-EP4 signaling acts directly on type 3 innate lymphoid cel...
Source: ScienceNOW - March 17, 2016 Category: Science Authors: Rodger Duffin Source Type: news

TET proteins help maintain genome integrity
(La Jolla Institute for Allergy and Immunology) Members of the TET (short for ten-eleven translocation) family have been known to function as tumor suppressors for many years, but how they keep a lid on the uncontrolled cell proliferation of cancer cells had remained uncertain. Now, researchers at the La Jolla Institute for Allergy and Immunology demonstrate that TET proteins collectively constitute a major class of tumor suppressors and are required to maintain genome instability. (Source: EurekAlert! - Medicine and Health)
Source: EurekAlert! - Medicine and Health - December 9, 2015 Category: Global & Universal Source Type: news

An In Vitro Assay for Substrate Translocation by FhaC in Liposomes
The two-partner secretion (TPS) pathway is used by gram-negative bacteria to secrete a large family of virulence exoproteins. Its name is derived from the fact that it involves two proteins, a secreted TpsA protein and a cognate TpsB transporter in the outer membrane. A typical TPS system is represented by the filamentous hemagglutinin FhaB (TpsA protein) and its transporter FhaC (TpsB protein) of Bordetella pertussis. Results from mutational analysis and heterologous expression experiments suggested that FhaC is essential for FhaB translocation across the outer membrane of bacteria. We have devised a cell-free biochemical...
Source: Springer protocols feed by Protein Science - October 6, 2015 Category: Biochemistry Source Type: news

Burn injury alters the intestinal microbiome and increases gut permeability and bacterial translocation - Earley ZM, Akhtar S, Green SJ, Naqib A, Khan O, Cannon AR, Hammer AM, Morris NL, Li X, Eberhardt JM, Gamelli RL, Kennedy RH, Choudhry MA.
Sepsis remains one of the leading causes of death in burn patients who survive the initial insult of injury. Disruption of the intestinal epithelial barrier has been shown after burn injury; this can lead to the translocation of bacteria or their products ... (Source: SafetyLit: All (Unduplicated))
Source: SafetyLit: All (Unduplicated) - July 15, 2015 Category: Global & Universal Tags: Burns, Electricity, Explosions, Fire, Scalds Source Type: news

Xp11.2 translocation renal cell carcinomas in young adults
Little is known about the biological behavior of Xp11.2 translocation renal cell carcinomas (RCCs) as few clinical studies have been performed using a large sample size.07/01/2015 (Source: Kidney Cancer Association)
Source: Kidney Cancer Association - July 1, 2015 Category: Cancer & Oncology Source Type: news

Purple Power For Your Brain
During one of my trips to Brazil a few years ago, I went in search of new healing herbs I could use at my wellness center. That’s when I discovered açaí berries. Now everyone’s talking about these berries. And they really are one of the richest sources in antioxidants, vitamins A, B, C, and E, minerals and omega-3s. In fact, I ended up including powdered açaí berries in one of my supplements. Back then, they were pretty much a novelty outside of South America, but now I even see frozen açaí berry smoothie packs when I’m in the grocery store. An...
Source: Al Sears, MD Natural Remedies - June 29, 2015 Category: Complementary Medicine Authors: Dr. Al Sears Tags: Brain Health Nutrition Acai berry anthocyanin extract brain-derived neurotrophic Parkinson’s disease Source Type: news

Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-assembly
We present here the various approaches that can be used for the detection and analysis of bacterial small noncoding RNA self-assemblies. (Source: Springer protocols feed by Biotechnology)
Source: Springer protocols feed by Biotechnology - April 25, 2015 Category: Biotechnology Source Type: news

Assay of Rab17 and Its Guanine Nucleotide Exchange Factor Rabex-5 in the Dendrites of Hippocampal Neurons
Neurons are functionally and morphologically compartmentalized into axons and dendrites, and the localization of specific proteins within these compartments is critical to the proper formation of neuronal networks, which includes neurite morphogenesis and synapse formation. The small GTPase Rab17 is specifically localized in dendrites and is not found in axons, and it regulates the dendrite morphogenesis and postsynaptic development of mouse hippocampal neurons. However, the spatiotemporal regulation of Rab17 is poorly understood. We recently identified Rabex-5, originally described as a Rab5-guanine nucleotide exchange fa...
Source: Springer protocols feed by Biochemistry - March 26, 2015 Category: Biochemistry Source Type: news

Methods for the Assembly and Analysis of In Vitro Transcription-Coupled-to-Translation Systems
RNA polymerase is a complex machinery, which is further embedded in interactions with other cellular components that interplay with either the transcribed DNA (DNA polymerases, topoisomerases, etc.) or the nascent RNA (RNA processing enzymes, ribosomes, etc.). In prokaryotes, coupling of transcription and translation is thought to play many regulatory roles but the mechanistic understanding of their interactions has been hindered by the lack of a defined experimental system. Here, we describe a pure transcription-coupled-to-translation system in which control of the ribosome has been achieved through its stepwise transloca...
Source: Springer protocols feed by Microbiology - February 13, 2015 Category: Microbiology Source Type: news

Monitoring Translocation of Multisubunit RNA Polymerase Along the DNA with Fluorescent Base Analogues
We describe two template DNA strand designs where translocation of RNA polymerase from a pre-translocation to a post-translocation state results in disruption of stacking interactions of fluorophore with neighboring bases, with a concomitant large increase in fluorescence intensity. (Source: Springer protocols feed by Microbiology)
Source: Springer protocols feed by Microbiology - February 13, 2015 Category: Microbiology Source Type: news

Mapping the Escherichia coli Transcription Elongation Complex with Exonuclease III
RNA polymerase interactions with the nucleic acids control every step of the transcription cycle. These contacts mediate RNA polymerase recruitment to promoters, induce pausing during RNA chain synthesis, and control transcription termination. These interactions are dissected using footprinting assays, in which a bound protein protects nucleic acids from the digestion by nucleases or modification by chemical probes. Exonuclease III is frequently employed to study protein–DNA interactions owing to relatively simple procedures and low background. Exonuclease III has been used to determine RNA polymerase position in tra...
Source: Springer protocols feed by Microbiology - February 13, 2015 Category: Microbiology Source Type: news

Expression of Gut-homing B7 Receptor on T CellsExpression of Gut-homing B7 Receptor on T Cells
This article analyzes microbial translocation and expression of the gut-homing B7 receptor on peripheral T cells in HIV-1-infected individuals. HIV Medicine (Source: Medscape Today Headlines)
Source: Medscape Today Headlines - February 9, 2015 Category: Consumer Health News Tags: HIV/AIDS Journal Article Source Type: news

Crucial protective role observed for farnesoid-x receptor in cholestatic liver injury
The farnesoid-X receptor (FXR), also known as the chief regulator of bile acid metabolism, is thought to play a role in some hepatobiliary and gastrointestinal disorders. A recent study has demonstrated dysfunctional intestinal FXR-signaling in a rat model of cholestatic liver injury, accompanied by intestinal bacterial translocation (BTL) and increased permeability and inflammation. Notably, a highly potent, selective FXR agonist obeticholic acid (INT-747) counteracted these effects, suggesting a potential new therapeutic avenue for liver disease. (Source: ScienceDaily Headlines)
Source: ScienceDaily Headlines - January 29, 2015 Category: Science Source Type: news

Crucial protective role observed for farnesoid-X receptor in cholestatic liver injury
(Elsevier Health Sciences) The farnesoid-X receptor (FXR), also known as the chief regulator of bile acid metabolism, is thought to play a role in some hepatobiliary and gastrointestinal disorders. In a study published in The American Journal of Pathology, researchers demonstrated dysfunctional intestinal FXR-signaling in a rat model of cholestatic liver injury, accompanied by intestinal bacterial translocation and increased permeability and inflammation. Notably, a highly potent, selective FXR agonist obeticholic acid counteracted these effects, suggesting a potential new therapeutic avenue for liver disease. (Source: Eur...
Source: EurekAlert! - Medicine and Health - January 29, 2015 Category: Global & Universal Source Type: news

Shell shock remains 'unsolved'
Conclusion This research aimed to shed light on the type of brain damage that blast exposure during military conflict may cause. Previous research suggested blast exposure can cause diffuse axonal injury, where the forces acting upon the brain cause tearing and damage of the long nerve fibres that connect different parts of the brain. This study found some supportive evidence suggesting this might be the case. Four of the five veterans with a history of blast injury did show this type of nerve fibre damage. Researchers also observed a distinctive "honeycomb" pattern of nerve fibre damage, which was not present...
Source: NHS News Feed - January 19, 2015 Category: Consumer Health News Tags: Neurology Mental health Source Type: news

An unholy alliance -- Colon cancer cells in situ co-opt fibroblasts in surrounding tissue to break out
(American Society for Cell Biology) In work to be presented at the ASCB/IFCB meeting in Philadelphia, researchers from the Institut Curie in Paris report that they have evidence of a coordinated attack on the basement membrane of human colon cells by cancer cells in situ and CAF cells in the extracellular matrix that begins long before the actual translocation of cancer cells. (Source: EurekAlert! - Cancer)
Source: EurekAlert! - Cancer - December 5, 2014 Category: Cancer & Oncology Source Type: news

Modification Interference Analysis of the Ribosome
RNAs are versatile molecules involved in myriad functions in the cell. To understand how a RNA molecule functions in the cell it is important to identify the nucleotides in the RNA molecule that are important for its structure and function. There are several biochemical methods such as footprinting, cross-linking, and modification interference analysis that can be used to study RNA–RNA and RNA–protein interactions. Ribosome is a classical example of a RNA–protein complex that has been extensively studied using these methods. Here, we describe a modification interference method that was used to identify ba...
Source: Springer protocols feed by Genetics/Genomics - October 30, 2014 Category: Genetics & Stem Cells Source Type: news

Studying nanocrystals by passing them through tiny pores
Researchers have now applied a cutting-edge technique for rapid gene sequencing toward measuring other nanoscopic structures. By passing nanoscale spheres and rods through a tiny hole in a membrane, the team was able to measure the electrical properties of those structures' surfaces. Their findings suggest new ways of using this technique, known as 'nanopore translocation,' to analyze objects at the smallest scale. (Source: ScienceDaily Headlines)
Source: ScienceDaily Headlines - September 26, 2014 Category: Science Source Type: news

Generation and Use of Site-Directed Chromosomal cyaA′ Translational Fusions in Salmonella enterica
CyaA from Bordetella pertussis is a calmodulin-dependent adenylate cyclase. Fusions to the catalytic domain of CyaA (CyaA′) are useful tools to detect translocation of type III secretion system effectors from gram-negative pathogens like Salmonella enterica. These fusions are usually generated using plasmids with strong promoters. Here, we describe a protocol to insert the CyaA′-encoding sequence in a specific site in the bacterial chromosome in order to get a monocopy fusion whose expression is driven by the native promoter. We also describe the procedure to detect translocation of a CyaA′ fusion into ma...
Source: Springer protocols feed by Infectious Diseases - September 25, 2014 Category: Infectious Diseases Source Type: news

Selexis SA to Present New Data and a Case Study at IBC’s Cell Line...
Data addresses key issues associated with effective translation, translocation and secretion of therapeutic proteins in mammalian cell lines(PRWeb July 30, 2014)Read the full story at http://www.prweb.com/releases/cell_line_development_and/engineering_selexis/prweb12057792.htm (Source: PRWeb: Medical Pharmaceuticals)
Source: PRWeb: Medical Pharmaceuticals - July 31, 2014 Category: Pharmaceuticals Source Type: news

Do metals that translocate to the brain exacerbate traumatic brain injury? - Kalinich JF, Kasper CE.
Metal translocation to the brain is strictly controlled and often prevented by the blood-brain barrier. For the most part, only those metals required to maintain normal function are transported into the brain where they are under tight metabolic control. F... (Source: SafetyLit: All (Unduplicated))
Source: SafetyLit: All (Unduplicated) - May 28, 2014 Category: Global & Universal Tags: Burns, Electricity, Explosions, Fire, Scalds Source Type: news

JCI online ahead of print table of contents for May 16, 2014
(Journal of Clinical Investigation) This release contains summaries, links to PDFs, and contact information for the following newsworthy papers published online, May 16, 2014 in the JCI: 'Targeting microbial translocation attenuates SIV-mediated inflammation,' 'Estrogen underlies sex-specific responses to sildenafil,' 'Vaccine-induced cell population inhibits SIV vaccine efficacy,' 'Enhancing efficacy of the cancer drug cetuximab,' 'Beta-catenin-regulated myeloid cell adhesion and migration determine wound healing,' 'Elevated sphingosine-1-phosphate promotes sickling and sickle cell disease progression,' and more. (Source:...
Source: EurekAlert! - Cancer - May 16, 2014 Category: Cancer & Oncology Source Type: news

Cell-Free NADPH Oxidase Activation Assays: “In Vitro Veritas”
The superoxide (O2 ∙−)-generating NADPH oxidase complex of phagocytes comprises a membrane-imbedded heterodimeric flavocytochrome, known as cytochrome b 558 (consisting of Nox2 and p22 phox ) and four cytosolic regulatory proteins, p47 phox , p67 phox , p40 phox , and the small GTPase Rac. Under physiological conditions, in the resting phagocyte, O2 ∙− generation is initiated by engagement of membrane receptors by a variety of stimuli, followed by specific signal transduction sequences leading to the translocation of...
Source: Springer protocols feed by Infectious Diseases - February 28, 2014 Category: Infectious Diseases Source Type: news

Clinical heterogeneity of Xp11 translocation renal cell carcinoma
Impact of fusion subtype, age, and stage12/09/2013 (Source: Kidney Cancer Association)
Source: Kidney Cancer Association - December 8, 2013 Category: Cancer & Oncology Source Type: news

Pest control, economic globalization and the involvement of policy makers
(Pensoft Publishers) With the increase in global trade, the potential for translocation of harmful pests, weeds, and pathogens capable of impacting our crops, livestock and natural resources also grows. A new special issue of NeoBiota journal, published following the 2012 meeting of the International Pest Risk Mapping Workgroup, explores the interface between pest risk science and policy to manage biological invasion risks. (Source: EurekAlert! - Biology)
Source: EurekAlert! - Biology - September 13, 2013 Category: Biology Source Type: news

Pulse Shape Analysis (PulSA) to Track Protein Translocalization in Cells by Flow Cytometry: Applications for Polyglutamine Aggregation
We describe the basic experimental details for performing PulSA using expression of GFP-tagged proteins, endogenous proteins labelled immunofluorescently, and organelle dyes. (Source: Springer protocols feed by Genetics/Genomics)
Source: Springer protocols feed by Genetics/Genomics - June 3, 2013 Category: Genetics & Stem Cells Source Type: news

Cell Biological Approaches to Investigate Polyglutamine-Expanded AR Metabolism
Spinal and bulbar muscular atrophy (SBMA) is a late-onset neurodegenerative disease caused by a polyglutamine expansion in the androgen receptor (AR). In vivo and in vitro studies have suggested that some steps of normal AR function and metabolism, such as hormone binding and nuclear translocation of the AR, are necessary for toxicity and aggregation of the mutant protein. Mutation of discreet functional domains of the AR and sites of posttranslational modification enable the detailed analysis of the role of AR function and metabolism in toxicity and aggregation of polyglutamine-expanded AR. This analysis could potentially...
Source: Springer protocols feed by Genetics/Genomics - June 3, 2013 Category: Genetics & Stem Cells Source Type: news

Beta Blockers Ameliorate GI Permeability in CirrhosisBeta Blockers Ameliorate GI Permeability in Cirrhosis
Nonselective beta-blocker therapy improves gastrointestinal permeability, decreases intestinal bacterial translocation, and reduces variceal bleeding in patients with cirrhosis and esophageal varices. Medscape Medical News (Source: Medscape Today Headlines)
Source: Medscape Today Headlines - May 2, 2013 Category: Consumer Health News Tags: Gastroenterology News Source Type: news