Identification of DNA-Methylated CpG Islands Associated With Gene Silencing in the Adult Body Tissues of the Ogye Chicken Using RNA-Seq and Reduced Representation Bisulfite Sequencing

Conclusion In conclusion, genes that display a correlation between DNA methylation and their expression in specific tissues are responsible for significant changes in not only in developmental stages but also in adult tissues. We made this finding by using tissues that are not in developmental stages, but have developed in adults. This can either represent a change that occurs at the developmental stage, or it can be the result of epigenetic changes. This study presents a methodology that can be used by other researchers using omics data combined with different types of NGS data, and provides them with the opportunity to use our transcriptome and DNA methylome landscapes. Ethics Statement This study was carried out in accordance with the guidelines of the Institutional Animal Care and Use Committee (IACUC) and was approved by the National Institute of Animal Science, Rural Development Administration, South Korea (Approval No: 2014-080). Animal preparation and experiments were conducted in accordance with protocols approved under the guidance of the IACUC. Author Contributions NK designed and supervised this project. W-JL and KK analyzed the data and drafted the manuscript. J-YK and SJ interpreted and visualized the results. All authors reviewed and approved the final manuscript. Funding This work was supported by grants from the National Research Foundation of Korea (NRF-2014M3C9A3064552) and the KRIBB Initiative Program and Agriculture Science and Technology Developme...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research