Autophagy Limits Inflammasome During Chlamydia pneumoniae Infection
In this study we found blocking autophagy led to increased CP growth in both macrophages and mouse embryonic fibroblasts. In vivo, loss of the autophagy elongation component ATG16L1 specifically in myeloid cells led to increased mortality in response to CP infection, characterized by greater numbers of neutrophils and dendritic cells, but no change in the CP burden in the lungs. This was accompanied by an increase in inflammasome-active macrophages and IL-1β production. While induction of autophagy in macrophages led to reduced CP growth in vitro, in vivo treatment with rapamycin led to increased mortality of infected mice, likely due to initial impairment in IL-1β production resulting in increased bacterial load. Materials and Methods Mice and Cell Lines C57BL/6 (wild-type [WT]) mice were purchased from Jackson Labs (Bar Harbor, ME) and then bred in the Cedars-Sinai Medical Center Comparative Medicine facility. Atg16l1fl/fl Lysmcre mice were obtained from Dr. David Shih, Cedars-Sinai medical Center (26). Atg16l1fl/fl mice were used as littermate controls. Eight- to ten-weeks-old female mice were used in all experiments in this study. WT, Atg16l1+/−, and Atg5−/− mouse embryonic fibroblasts (MEF)s were obtained from Dr. Jae Jung (University of Southern California) (27) and grown in DMEM media containing 10% FBS. In some cases, 1 μg/ml cycloheximide (Sigma Aldrich, St. Louis MO), MEM amino acids (Sigma Aldrich, St. Louis MO), or Na-pyruvate (Si...
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Stacey Nagy told CNN's Erin Burnett she wrote an obituary for her husband's coronavirus death where she blamed President Trump, Texas Gov. Abbott and those who do not wear masks because she felt a need to "put blame where it belonged."
In this study, we investigated the potential effects of disocin on amyloid-β peptide (Aβ1-42) oligomers-treated HT22 cells. Aβ1-42 oligomers induced great neurotoxicity to HT22 cells as examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results of terminal deoxynucleoitidyl transferase-mediated deoxyuridine triphosphate biotin nich end labeling (TUNEL) staining and flow cytometry indicated that Aβ1-42 oligomers led to increased apoptosis and generation of reactive oxygen species (ROS) in HT22 cells. However, dioscin could remarkably inhibit the neurotoxicity induce...
This article summarizes the up-to-date information regarding the phytochemistry, pharmacology, processing, toxicity and safety of Rou Cong Rong to reveal its pharmacodynamic basis and potential therapeutic effects, which could be of great value for its use in future research. PMID: 32741910 [PubMed - in process]
Those suffering from cancer, heart conditions, strokes, diabetes and lung disease are among the worst affected by delays to their diagnosis and care, a survey of senior doctors reveals.
The objective of this study was to report the incidence of alopecia arising in patients treated with vismodegib, assessing its characteristics, grade of severity, and time of onset.Skin Appendage Disord
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