Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
In conclusion, the method of NK cell transfection described in our present study is highly efficient, does not require expensive dedicated structures necessary for viral transduction and avoids possible risks associated with the use of viral vectors. Importantly, it may be applied to NK cells or NK-92 cell line, greatly improving their anti-tumor activity and providing a new NK cell-based platform for new protocols of adoptive immuno-therapy of cancer.
Ethics Statement
The Ethical Committee of IRCCS Bambino Gesù Pediatric Hospital approved the study (825/2014).
Author Contributions
TI designed and performed research, interpreted data, and wrote the article. FM, FB, and NT performed experiments. AP, CC, and FL reviewed the manuscript. CQ and BD developed CAR constructs and revised the manuscript. PV and LM designed research and wrote the paper.
Funding
The present study has been supported by the following grants: Associazione Italiana per la Ricerca sul Cancro (AIRC) IG 2014 Id. 15283 (LM), IG 2017 Id. 19920 (LM), Special Project 5X1000 no. 21147 (LM), and Ministero della Salute GR-2013-02356568 (PV). NT is recipient of a fellowship awarded by AIRC.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
We thank Ezio Giorda and the Flow Cytometry core facility of IRCCS Bambino Gesù Childre...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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