Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

This study included 18 buffy coats collected from volunteer blood donors admitted to the blood transfusion service of IRCCS Bambino Gesù Pediatric Hospital after obtaining informed consent. The Ethical Committee of IRCCS Bambino Gesù Pediatric Hospital approved the study (825/2014) and conducted in accordance with the ethical principles stated in the Declaration of Helsinki. Cells Lines and Cell Culture NK-92 (malignant non-Hodgkin's lymphoma), K562 (chronic myelogenous leukemia, CD19−), Jurkat (acute T cell leukemia, CD19−) Karpas 299 (Human Non-Hodgkin's Ki-positive Large Cell Lymphoma, CD19−), Nalm-8 (Lymphoblastic Leukemia, CD19+) Raji (Burkitt's Lymphoma, CD19+), and DAUDI (Burkitt's Lymphoma, CD19+) cell lines were purchased from American Type Culture Collection (ATCC, Rockville, MD). K562, Karpas 299, Jurkat, Nalm-8, Raji and Daudi cells were cultured in RPMI 1640 supplemented with 2 mM l-glutamine, 1% penicillin-streptomycin-neomycin mixture and 10% heat-inactivated Fetal Calf Serum. For NK-92 20% of FCS and 50 ng/ml of recombinant human IL-2 (Proleukin; Chiron Therapeutics, Emeryville, CA) were added at the culture medium. Peripheral blood mononuclear cell (PBMC) were obtained from buffy coats after density gradient centrifugation over Ficoll Lympholyte®-H (Cederlane, Burlington, Canada). Highly purified (≥ 98%) NK cells were subsequently obtained by depletion of non-NK cells using the Miltenyi...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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Publication date: Available online 2 August 2020Source: Cirugía Española (English Edition)Author(s): Sheila Serra-Pla, Anna Pallisera-Lloveras, Laura Mora-López, Ana Granados Maturano, Sebastian Gallardo, Carmen del Pino Zurita, Xavier Serra-Aracil
Source: Cirugia Espanola - Category: Surgery Source Type: research
Solar ultraviolet radiation (UVR) induces the production of vitamin D (VD), but it also causes erythema and skin cancer among other diseases. The amount of UVR to get benefit and avoid harm is unknown. Some principles were proposed to provide accessible information on how to avoid overexposure to the Sun. They are (1) the Shadow Rule (SR); (2) the UV Index (UVI); and (3) the Slip, Slop, Slap, Seek, Slide (5S) steps. This work discusses these principles in the tropics, considering measurements of solar UVR, total ozone column, and cloudiness. Indeed, the SR leads to overexposures. The UVI is adequate for cloudless skies, bu...
Source: Health Physics - Category: Radiology Tags: NOTES Source Type: research
The excess relative risk (ERR) of mortality for circulatory disease among nuclear workers was reanalyzed by taking into consideration the annual dose as the dose rate using publicly available epidemiological data of the Hanford site dedicated to the cohort study of nuclear workers in the US, the UK, and Canada. Values of the dose rate (cut-points) were chosen at 2 mSv y−1 intervals from 2 to 40 mSv y−1, and risk estimates were made for 32,988 workers, considering the doses accumulated below and above each cut-point to have different effects. Similarly to that in the previous study for cancer by Sasaki et al., e...
Source: Health Physics - Category: Radiology Tags: PAPERS Source Type: research
Conclusions: Patients with hematologic tumors had an increase in body weight, height, and fat mass, which was not identified in the solid tumor group. The positive correlation between anthropometry (triceps skinfold thickness and arm muscle circumference), deuterium oxide dilution, and bioelectric impedance analysis shows the applicability of anthropometry in clinical practice.RESUMO Objetivo: Verificar mudan ças no estado nutricional de crianças com câncer antes e após o tratamento quimioterápico e avaliar a correlação entre diluição de óxido de deut&eac...
Source: Revista Paulista de Pediatria - Category: Pediatrics Source Type: research
Lufthansa said it's also making a rapid Covid-19 test available at Frankfurt Airport, calling it an important first step "to restart international travel." Here's how the German airline is doing it.
Source: bizjournals.com Health Care:Pharmaceuticals headlines - Category: Pharmaceuticals Authors: Source Type: news
Publication date: Available online 2 August 2020Source: Pathology - Research and PracticeAuthor(s): Raima A. Memon, Carlos N Prieto Granada, Shi Wei
Source: Pathology Research and Practice - Category: Pathology Source Type: research
The objective of this study was to evaluate the characteristics and oncologic outcomes of a single institution series of chondrosarcoma of pelvis and extremities in patients who were 21 years or younger. Between January 2006 and December 2016, we operated on 249 chondrosarcomas of the pelvis and extremities. Eleven (4%) of these were 21 years or younger. The mean age was 18 years (range 14–21 years). There were eight males and three females. Four occurred in the pelvis. The other sites were humerus (three) and one each in the scapula, clavicle, metatarsal and proximal femur. There were five primary chondrosarcomas an...
Source: Journal of Pediatric Orthopaedics B - Category: Orthopaedics Tags: Bone Tumours Source Type: research
This article reviews the history of syphilis, including the unethical studies undertaken in the past; current epidemiology; treatme nt guidelines; and strategies to reduce new infections.
Source: Nursing Clinics of North America - Category: Nursing Authors: Source Type: research
This article provides an overview of the incidence and prevalence of sexually transmitted infections in pregnant women in the United States. It discusses screening guidelines and best practices related to treatments. Safety, pharmacology, and monitoring of both the woman and her fetus are detailed.
Source: Nursing Clinics of North America - Category: Nursing Authors: Source Type: research
According to the UK's Department of Health, the British government has procured "millions" of two separate coronavirus tests that can detect not only coronavirus but several other viruses, providing "on-the-spot results in under 90 minutes." Dr. Ashish Jha, Director of the Harvard Global Health Institute, says the US has the ability to do the same thing.
Source: CNN.com - Health - Category: Consumer Health News Source Type: news
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