TNF Induces Pathogenic Programmed Macrophage Necrosis in Tuberculosis through a Mitochondrial-Lysosomal-Endoplasmic Reticulum Circuit
Publication date: Available online 29 August 2019Source: CellAuthor(s): Francisco J. Roca, Laura J. Whitworth, Sarah Redmond, Ana A. Jones, Lalita RamakrishnanSummaryNecrosis of infected macrophages constitutes a critical pathogenetic event in tuberculosis by releasing mycobacteria into the growth-permissive extracellular environment. In zebrafish infected with Mycobacterium marinum or Mycobacterium tuberculosis, excess tumor necrosis factor triggers programmed necrosis of infected macrophages through the production of mitochondrial reactive oxygen species (ROS) and the participation of cyclophilin D, a component of the mi...
Source: Cell - August 30, 2019 Category: Cytology Source Type: research

[Respiratory symptoms in people attended in health facilities of the Ministry of Health in Lima, Peru].
CONCLUSIONS.: The percentage of RS in the Ministry of Health's health facilities in Lima was less than the expected 5%, ranging from 0.8% in Callao to 1.5% in Lima City. The percentage of tuberculosis among coughers (lasting 7-14 days) and RS is consistent with the findings of other local studies. Consideration should be given to lowering the RS target to less than 5% and expanding tuberculosis screening to patients with cough ≥7 days at the Health Divisions with high tuberculosis incidence. PMID: 31460631 [PubMed - in process] (Source: Revista Peruana de Medicina de Experimental y Salud Publica)
Source: Revista Peruana de Medicina de Experimental y Salud Publica - August 30, 2019 Category: International Medicine & Public Health Tags: Rev Peru Med Exp Salud Publica Source Type: research

Screening for Tuberculosis Among Asylum Seekers.
Authors: Joob B, Wiwanitkit V PMID: 31455113 [PubMed - as supplied by publisher] (Source: COPD: Journal of Chronic Obstructive Pulmonary Disease)
Source: COPD: Journal of Chronic Obstructive Pulmonary Disease - August 30, 2019 Category: Respiratory Medicine Tags: COPD Source Type: research

Evaluation of emerging infectious disease and the importance of SINAN for epidemiological surveillance of Venezuelans immigrants in Brazil
Publication date: Available online 28 August 2019Source: The Brazilian Journal of Infectious DiseasesAuthor(s): Mário Maciel de Lima Junior, Gabbrielle Almeida Rodrigues, Maysa Ruiz de LimaAbstractBackgroundFollowing socio-economic turmoil in Venezuela, millions of the Venezuelan people are migrating to the neighboring Latin American countries including Brazil. Besides the social and economic burden of these migrants, Brazil must manage the health-related issues arising as consequence of these Venezuelan migrants.Poor health services in Venezuela along with unhygienic travelling conditions, lack of food (malnutritio...
Source: The Brazilian Journal of Infectious Diseases - August 30, 2019 Category: Infectious Diseases Source Type: research

Detection of splenic microabscesses with ultrasound as a marker for extrapulmonary tuberculosis in patients with HIV: A systematic review.
CONCLUSIONS: Ultrasound evaluation of the spleen in patients with HIV and symptoms suggestive of TB in endemic regions is a viable diagnostic adjunct. Ultrasound detection of splenic microabscesses in HIV patients is probably sufficient indication to initiate TB treatment prior to obtaining culture data. Strong conclusions cannot be drawn owing to the high heterogeneity of this small number of studies. PMID: 31456550 [PubMed - in process] (Source: South African Medical Journal)
Source: South African Medical Journal - August 30, 2019 Category: African Health Tags: S Afr Med J Source Type: research

Tuberculosis infection control in a South African rural regional hospital emergency centre: Prioritisation for patients and healthcare workers.
This article examines key challenges to effective TB infection control from a rural regional hospital perspective. It uses the Eden District in Western Cape Province as an example to share lessons learnt. This quality-improvement project identifies four priorities for improving TB infection control in George Hospital and the Eden District: (i) prioritising TB infection control in local policy; (ii) improving the quality of TB screening in the emergency centre; (iii) increasing the number of TB patients followed up; and (iv) implementing TB infection control training for all staff. This project demo...
Source: South African Medical Journal - August 30, 2019 Category: African Health Tags: S Afr Med J Source Type: research

The active site of the Mycobacterium tuberculosis branched-chain amino acid biosynthesis enzyme dihydroxyacid dehydratase contains a 2Fe-2S cluster [Enzymology]
Iron–sulfur clusters are protein cofactors with an ancient evolutionary origin. These clusters are best known for their roles in redox proteins such as ferredoxins, but some iron–sulfur clusters have nonredox roles in the active sites of enzymes. Such clusters are often prone to oxidative degradation, making the enzymes difficult to characterize. Here we report a structural and functional characterization of dihydroxyacid dehydratase (DHAD) from Mycobacterium tuberculosis (Mtb), an essential enzyme in the biosynthesis of branched-chain amino acids. Conducting this analysis under fully anaerobic conditions, we s...
Source: Journal of Biological Chemistry - August 30, 2019 Category: Chemistry Authors: Ghader Bashiri, Tyler L. Grove, Subray S. Hegde, Thomas Lagautriere, Gary J. Gerfen, Steven C. Almo, Christopher J. Squire, John S. Blanchard, Edward N. Baker Tags: Protein Structure and Folding Source Type: research

Stamping out Tuberculosis: The Importance of Diagnostic Innovation and Effective Implementation
Annals of the American Thoracic Society,Volume 16, Issue 9, Page 1112-1113, September 2019. (Source: Annals of the American Thoracic Society)
Source: Annals of the American Thoracic Society - August 30, 2019 Category: Respiratory Medicine Authors: Ruvandhi R. Nathavitharana Source Type: research

Paediatric tuberculosis diagnosis using Mycobacterium tuberculosis real-time polymerase chain reaction assay: protocol for systematic review and meta-analysis
DiscussionTo our knowledge, this is the first systematic review and meta-analysis assessing the detection of MTB from all clinical sample types using real-time polymerase chain reaction assay in paediatric population. This review will make available evidence on the accuracy, approach, and interpretation of results of this assay in the context of MTB diagnosis which will meet an urgent need, considering the challenges of MTB diagnosis in paediatrics.Systematic review registrationPROSPERO CRD42018104052 (Source: Systematic Reviews)
Source: Systematic Reviews - August 30, 2019 Category: International Medicine & Public Health Source Type: research

Detection and decontamination of Mycobacterium chimaera and other non-tuberculosis mycobacteria in heater-cooler devices used in cardiopulmonary bypass: a Manufacturer and National guidelines summary, and a potential resolution to the problem requiring further investigation.
Abstract Since 2011, invasive infections due to Mycobacterium chimaera have been diagnosed in patients with previous cardiac surgery. The use of heating/cooling devices (heater-cooler units), necessary to regulate the blood temperature in extra-corporeal circulation, has been identified as a source of contaminated aerosol. Adhering to a strict maintenance program according to the manufacturer's instructions for use including sanitizing is essential to reduce the risk. Current manufacturer guidelines and national recommendations for detection and decontamination of Mycobacterium chimaera and other non-tuberculosis ...
Source: Perfusion - August 30, 2019 Category: Cardiovascular & Thoracic Surgery Authors: Casini B, Tuvo B, Totaro M, Baggiani A, Privitera G Tags: Perfusion Source Type: research

Structural insight into conformational dynamics of non-active site mutations in KasA: A Mycobacterium tuberculosis target protein.
This study provides a better understanding of the structural dynamic behaviour of KasA mutants, thereby facilitating the need to find a novel and potent inhibitor against Mtb. PMID: 31476406 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - August 30, 2019 Category: Genetics & Stem Cells Authors: Jayaraman M, Rajendra SK, Krishna R Tags: Gene Source Type: research

The rollout of Community ART Refill Groups in Zimbabwe: a qualitative evaluation.
CONCLUSIONS: We found that healthcare workers and clients overwhelmingly perceive CARGs as beneficial. This evaluation demonstrates that the CARG model can be successfully implemented on a national scale. These early results suggest that CARGs may be able to simultaneously improve clinical outcomes and reduce the workload of healthcare workers distributing ART. PMID: 31454178 [PubMed - in process] (Source: Journal of the International AIDS Society)
Source: Journal of the International AIDS Society - August 29, 2019 Category: Infectious Diseases Tags: J Int AIDS Soc Source Type: research

Predictive factors for neurological deficit in patients with spinal tuberculosis.
CONCLUSION: The predictive factors for neurological deterioration in spinal TB patients are signal cord change and notable Cobb angle. Surgery should be considered in patients who present with these factors before the development of neurological deterioration. PMID: 31451078 [PubMed - in process] (Source: Journal of Orthopaedic Surgery)
Source: Journal of Orthopaedic Surgery - August 29, 2019 Category: Orthopaedics Authors: Sae-Jung S, Wongba N, Leurmprasert K Tags: J Orthop Surg (Hong Kong) Source Type: research

Drug-Susceptible and Multidrug-Resistant Mycobacterium tuberculosis in a Single Patient.
Abstract A patient who had initial infection with mixed strains of drug-susceptible and multidrug-resistant tuberculosis was presumed to have acquired drug resistance before confirmation that sequential strains were genotypically distinct. Transmitted infection with mixed strains is likely underappreciated; identifying these infections requires spoligotyping and whole-genome sequencing. PMID: 31454310 [PubMed - as supplied by publisher] (Source: Emerging Infectious Diseases)
Source: Emerging Infectious Diseases - August 29, 2019 Category: Infectious Diseases Authors: Baffoe-Bonnie A, Houpt ER, Turner L, Dodge D, Heysell SK Tags: Emerg Infect Dis Source Type: research

The Application Value of Serum HE4 in the Diagnosis of Lung Cancer.
Authors: Wang Y, Wang Z, Ding Y, Sun F, Ding X Abstract Background: To investigate the clinical value of HE4 detection in the diagnosis of lung cancer and the clinical significance of combined detection with CEA, NSE and CYFRA21-1. Methods: 90 cases of lung cancer, 30 cases of pulmonary tuberculosis, 30 cases of pneumonia and 30 cases of health physical examination were selected. The levels of serum HE4, CYFRA21-1, CEA and NSE were detected by electrochemiluminescence method. Statistical analysis was performed to observe the sensitivity and specificity. Results: The levels of serum HE4, CEA, NSE and CYFRA21-1 in lu...
Source: Asian Pacific Journal of Cancer Prevention - August 29, 2019 Category: Cancer & Oncology Tags: Asian Pac J Cancer Prev Source Type: research

Review on antibacterial activity of Himalayan medicinal plants traditionally used to treat pneumonia and tuberculosis
AbstractObjectivesThe main objective of this review was to collect scattered literature on ethnomedicinal plants used to treat pneumonia and tuberculosis in the Himalayan region and theirin ‐vitro validation against bacterial pathogens.Key findingsCurrent review contains information on ethnomedicines of total 137 plants from Himalaya region. Out of these, 59 plants have been studiedin vitro against bacteria while seven plants extracts have been checked for their toxicological effects. The most commonly used plant families for pneumonia and tuberculosis therapy in the study region were Asteraceae, Bignoniaceae and Fabacea...
Source: Journal of Pharmacy and Pharmacology - August 29, 2019 Category: Drugs & Pharmacology Authors: Muhammad Adnan, Shandana Ali, Khushboo Sheikh, Rahila Amber Tags: Review Source Type: research

QuantiFERON TB Gold Plus for the diagnosis of tuberculosis: a systematic review and meta-analysis
Estimated 2017 tuberculosis (TB) incidence is 10 million and mainly depends on the reservoir of individuals with latent TB infection (LTBI). Quantiferon ®-TB Gold in-Tube (QFT-GIT) is one of the tests used for LTBI detection. Since 2015 a new version, Quantiferon®-TB Gold Plus (QFT-Plus) is available. Objectives: to perform a systematic review and meta-analysis to assess the diagnostic accuracy for TB of QFT-Plus compared to QFT-GIT. Methods: PubM ed and Scopus were used to detect records related to predefined strings from 2015 to 2018. (Source: Journal of Infection)
Source: Journal of Infection - August 29, 2019 Category: Infectious Diseases Authors: Giovanni Sotgiu, Laura Saderi, Elisa Petruccioli, Stefano Aliberti, Andrea Piana, Linda Petrone, Delia Goletti Source Type: research

Tuberculosis and the Acute Abdomen: An Evaluation of the National Inpatient Sample
Surgical Infections, Ahead of Print. (Source: Surgical Infections)
Source: Surgical Infections - August 29, 2019 Category: Surgery Authors: Joseph D. Forrester Peter Cha Lakshika Tennakoon Kristan Staudenmayer Source Type: research

Adjuvant ‐induced arthritis affects testes and ventral prostate of Wistar rats
ConclusionAIA effects on reproductive tissues may be related to both hypoandrogenism and other direct inflammatory mechanisms. Possibly, these AIA effects on the testes and prostate occur at a stage in which the inflammatory process is most active, about 15 –20 days after induction, remaining evident until the 40th day. (Source: Andrology)
Source: Andrology - August 29, 2019 Category: Urology & Nephrology Authors: C. R. Santos, A. C. A. Benjamin, A. B. Chies, R. F. Domeniconi, G. P. Zochio, M. A. Spadella Tags: Original Article Source Type: research

Quantitative determination of characteristic components from compound of Lysionotus pauciflorus Maxim. by LC-MS/MS and its application to a pharmacokinetic study
Publication date: Available online 27 August 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Caijuan Liang, Jintuo Yin, Yinling Ma, Xia zhang, Lantong ZhangAbstractTuberculosis of cervical lymph nodes is called scrofula in Traditional Chinese Medicine (TCM). Clinical manifestation is that unilateral or bilateral neck can have multiple enlarged lymph nodes of different sizes. Current therapeutic drugs include Lysionotus pauciflorus Maxim. tablets and compound of Lysionotus pauciflorus Maxim., which have a significant effect on tuberculosis of cervical lymph nodes. This compound is composed of three h...
Source: Journal of Pharmaceutical and Biomedical Analysis - August 28, 2019 Category: Drugs & Pharmacology Source Type: research

Rice bodies in a pediatric patient with juvenile idiopathic arthritis and latent tuberculosis: case report and literature review
No abstract available (Source: Current Orthopaedic Practice)
Source: Current Orthopaedic Practice - August 28, 2019 Category: Orthopaedics Tags: Case Reports Source Type: research

Childhood tuberculosis and treatment outcomes in Accra: a retrospective analysis
Tuberculosis (TB) is a leading cause of death in children and adults. Unlike for adults, there is paucity of data on childhood TB in several countries in Africa. The study objective was to assess the character... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - August 28, 2019 Category: Infectious Diseases Authors: Sally-Ann Ohene, Sarah Fordah and Prince Dela Boni Tags: Research article Source Type: research

Addressing challenges in tuberculosis adherence via performance-based payments for integrated case management: protocol for a cluster randomized controlled trial in Georgia
Tuberculosis is one of the greatest global health concerns and disease management is challenging particularly in low- and middle-income countries. Despite improvements in addressing this epidemic in Georgia, t... (Source: Trials)
Source: Trials - August 28, 2019 Category: General Medicine Authors: Ivdity Chikovani, Karin Diaconu, Predrag Duric, Lela Sulaberidze, Maia Uchaneishvili, Nuredin Ibrahim Mohammed, Akaki Zoidze and Sophie Witter Tags: Study protocol Source Type: research

False rifampicin resistant results using Xpert MTB/RIF on urine samples in hospitalised HIV-infected patients
Conclusion: Urine Xpert testing showed a high proportion of false rifampicin resistance results. Urine Xpert rifampicin resistant results should be interpreted cautiously and confirmed when possible. (Source: Southern African Journal of HIV Medicine)
Source: Southern African Journal of HIV Medicine - August 28, 2019 Category: African Health Authors: Charlotte Schutz Source Type: research

Methyl arachidonyl fluorophosphonate inhibits Mycobacterium  tuberculosis thioesterase TesA and globally affects vancomycin susceptibility
Phthiocerol dimycocerosates and phenolic glycolipids (PGL) are considered as major virulence elements ofMycobacterium  tuberculosis, in particular because of their involvement in cell wall impermeability and drug resistance. The biosynthesis of these waxy lipids involves multiple enzymes, including thioesterase A (TesA). We observed that purified recombinantM.  tuberculosis TesA is able to dimerize in the presence of palmitoyl ‐CoA and our 3D structure model of TesA with this acyl‐CoA suggests hydrophobic interaction requirement for dimerization. Furthermore, we identified that methyl arachidonyl fluorophosph...
Source: FEBS Letters - August 28, 2019 Category: Biochemistry Authors: Dong Yang, Guy Vandenbussche, Didier Vertommen, Damien Evrard, Romany Abskharon, Jean ‐François Cavalier, Gilles Berger, Stéphane Canaan, Mohammad Shahneawz Khan, Sheng Zeng, Alexandre Wohlkönig, Martine Prévost, Patrice Soumillion, Vé Tags: Research Article Source Type: research

Identification of Differentially Expressed Genes Associated with Lymph Node Tuberculosis by the Bioinformatic Analysis Based on a Microarray
Journal of Computational Biology, Ahead of Print. (Source: Journal of Computational Biology)
Source: Journal of Computational Biology - August 28, 2019 Category: Biology Authors: Zhenan Zhang Yuqin Liu Wei Wang Yue Xing Nanyang Jiang Hongrui Zhang Hanwen Zhang Lei He Wei Yue Lianju Jiang Kaili Wang Source Type: research

Adherence to antiretroviral therapy and the associated factors among people living with HIV/AIDS in Northern Peru: a cross-sectional study.
CONCLUSIONS: Self-reported adherence appeared to be low and the use of first-line therapy is not being prescribed homogeneously. Factors associated with nonadherence are both medical and behavioral, such as having tuberculosis, pausing ART, or experiencing discomfort with ART. The Peruvian government needs to update national technical standards, monitor medication availability, and provide education to health care professionals in alignment with evidence-based guidelines and international recommendations. Instruments to measure adherence need to be developed and evaluated for use in Latin America. PMID: 31462291 ...
Source: AIDS Research and Therapy - August 28, 2019 Category: Infectious Diseases Authors: Leyva-Moral JM, Loayza-Enriquez BK, Palmieri PA, Guevara-Vasquez GM, Elias-Bravo UE, Edwards JE, Feijoo-Cid M, Davila-Olano LY, Rodriguez-Llanos JR, Leon-Jimenez FE Tags: AIDS Res Ther Source Type: research

Contact tracing strategies in household and congregate environments to identify cases of tuberculosis in low- and moderate-incidence populations.
CONCLUSIONS: This Cochrane Review highlights the lack of research in support of the current contact tracing method and the need for RCTs to compare new methods such as social network analysis to improve contact tracing processes. PMID: 31461540 [PubMed - as supplied by publisher] (Source: Cochrane Database of Systematic Reviews)
Source: Cochrane Database of Systematic Reviews - August 28, 2019 Category: General Medicine Authors: Braganza Menezes D, Menezes B, Dedicoat M Tags: Cochrane Database Syst Rev Source Type: research

Methyl arachidonyl fluorophosphonate inhibits Mycobacterium  tuberculosis thioesterase TesA and globally affects vancomycin susceptibility
Phthiocerol dimycocerosates and phenolic glycolipids (PGL) are considered as major virulence elements ofMycobacterium  tuberculosis, in particular because of their involvement in cell wall impermeability and drug resistance. The biosynthesis of these waxy lipids involves multiple enzymes, including thioesterase A (TesA). We observed that purified recombinantM.  tuberculosis TesA is able to dimerize in the presence of palmitoyl ‐CoA and our 3D structure model of TesA with this acyl‐CoA suggests hydrophobic interaction requirement for dimerization. Furthermore, we identified that methyl arachidonyl fluorophosph...
Source: FEBS Letters - August 28, 2019 Category: Biochemistry Authors: Dong Yang, Guy Vandenbussche, Didier Vertommen, Damien Evrard, Romany Abskharon, Jean ‐François Cavalier, Gilles Berger, Stéphane Canaan, Mohammad Shahneawz Khan, Sheng Zeng, Alexandre Wohlkönig, Martine Prévost, Patrice Soumillion, Vé Tags: Research Article Source Type: research

Heterogeneity in the cytokine profile of tuberculosis - diabetes co-morbidity.
Abstract Tuberculosis - diabetes (TB-DM) co-morbidity is characterized by heterogeneity in clinical and biochemical parameters between newly diagnosed diabetic individuals with TB (TB-NDM) and known diabetic individuals at incident TB (TB-KDM). However, the immunological profile underlying this heterogeneity is not explored. To identify the cytokine profiles in TB-NDM and TB-KDM individuals, we examined the plasma cytokine levels as well as TB-antigen stimulated levels of pro-inflammatory cytokines. TB-KDM individuals exhibit significantly higher levels of IFNγ, IL-2, TNFα, IL-17A, IL-1α, IL-1&be...
Source: Cytokine - August 28, 2019 Category: Molecular Biology Authors: Kumar NP, Moideen K, Nancy A, Viswanathan V, Shruthi BS, Sivakumar S, Natarajan M, Kornfeld H, Babu S Tags: Cytokine Source Type: research

Evaluation of emerging infectious disease and the importance of SINAN for epidemiological surveillance of Venezuelans immigrants in Brazil.
Abstract BACKGROUND: Following socio-economic turmoil in Venezuela, millions of the Venezuelan people are migrating to the neighboring Latin American countries including Brazil. Besides the social and economic burden of these migrants, Brazil must manage the health-related issues arising as consequence of these Venezuelan migrants. Poor health services in Venezuela along with unhygienic travelling conditions, lack of food (malnutrition) and potable water, unhealthy and overcrowded refugee camps or shelters, poor availability of medical services have made the migrant Venezuelan population susceptible to various dis...
Source: Braz J Infect Dis - August 28, 2019 Category: Infectious Diseases Authors: Junior MML, Rodrigues GA, Lima MR Tags: Braz J Infect Dis Source Type: research

Lung ultrasonography in pulmonary tuberculosis: Integrating chest radiology? Authors' reply.
iati C PMID: 31473046 [PubMed - as supplied by publisher] (Source: European Journal of Internal Medicine)
Source: European Journal of Internal Medicine - August 28, 2019 Category: Internal Medicine Authors: Authors' reply. Tags: Eur J Intern Med Source Type: research

Mycobacterial infections with Ruxolitinib: A retrospective pharmacovigilance review
ConclusionPatients on Ruxolitinib are at increased risk of developing MTB and AMI. Clinicians should be aware of this risk and consider screening patients for latent MTB prior to initiating Ruxolitinib. (Source: Clinical Lymphoma Myeloma and Leukemia)
Source: Clinical Lymphoma Myeloma and Leukemia - August 27, 2019 Category: Cancer & Oncology Source Type: research

Drug-Susceptible and Multidrug-Resistant Mycobacterium tuberculosis in a Single Patient
A. Baffoe-Bonnie et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - August 27, 2019 Category: Infectious Diseases Source Type: research

Clinical profile, drug resistance pattern and treatment outcomes of abdominal tuberculosis patients in western india
CONCLUSION: Abdominal tuberculosis with wide spectrum of presentation, can still be managed with early diagnosis and treatment even in patients with sub acute intestinal obstruction. Weight gain or resolving symptoms were considered early markers of treatment response. Patients with stricture can become asymptomatic with medical treatment alone.RESUMO CONTEXTO: A tuberculose abdominal é um problema crescente no mundo em desenvolvimento. OBJETIVO: O objetivo do estudo foi descrever as apresentações clínicas, o padrão de resistência a fármacos e os desfechos de tratamento da tu...
Source: Arquivos de Gastroenterologia - August 27, 2019 Category: Gastroenterology Source Type: research

Comparison of pleural effusion features and biomarkers between talaromycosis and tuberculosis in non-human immunodeficiency virus-infected patients
This study aimed to identify the characteristics of talaromycosis pleur... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - August 27, 2019 Category: Infectious Diseases Authors: Ye Qiu, Wen Zeng, Hui Zhang, Xiaoning Zhong, Shudan Tang and Jianquan Zhang Tags: Research article Source Type: research

Acceptability of mandatory tuberculosis notification among private practitioners in Yogyakarta, Indonesia
Indonesia ranks second globally in the number of cases not reported to the National Tuberculosis Control Program, accounting for 11% of the total cases lost worldwide. In 2016, the Ministry of Health has issue... (Source: BMC Research Notes)
Source: BMC Research Notes - August 27, 2019 Category: Research Authors: Ari Kurniawati, Retna S. Padmawati and Yodi Mahendradhata Tags: Research note Source Type: research

High Activation of γδ T Cells and the γδ2pos T-Cell Subset Is Associated With the Onset of Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome, ANRS 12153 CAPRI NK
Background: HIV-1 and M. tuberculosis co-infected patients are commonly at risk of immune reconstitution inflammatory syndrome (IRIS) when initiating antiretroviral treatment (ART). Evidence indicates that innate immunity plays a role in TB-IRIS. Here, we evaluated the phenotype of γδ T cells and invariant NKT cells in tuberculosis-associated IRIS. Methods: Forty-eight HIV+/TB+ patients (21 IRIS) and three control groups: HIV-/TB- (HD, n=11), HIV+/TB- (n=26), and HIV-/TB+ (n=22) were studied. Samples were taken at ART initiation (week 2 of anti-tuberculosis treatment) and at the diagnosis of IRIS for HIV+/TB+;...
Source: Frontiers in Immunology - August 27, 2019 Category: Allergy & Immunology Source Type: research

Utility of Repeat Latent Tuberculosis Testing with QuantiFERON-TB Gold test in Psoriasis Patients Treated with TNF- α inhibitors at a Single U.S. Institution.
This article is protected by copyright. All rights reserved. PMID: 31452192 [PubMed - as supplied by publisher] (Source: The British Journal of Dermatology)
Source: The British Journal of Dermatology - August 27, 2019 Category: Dermatology Authors: Ya J, Khanna U, Havele S, Fernandez AP Tags: Br J Dermatol Source Type: research

Integrated target-based and phenotypic screening approaches for the identification of anti-tubercular agents that bind to the mycobacterial adenylating enzyme MbtA.
Abstract Iron is essential for the pathogenicity and virulence of Mycobacterium tuberculosis, which synthesises salicyl-capped siderophores (mycobactins) to acquire this element from the host. MbtA is the adenylating enzyme that catalyses the initial reaction of mycobactins' biosynthesis and is solely expressed by mycobacteria. A 3,200-member library comprised of lead-like, structurally-diverse compounds was screened against M. tuberculosis for whole-cell inhibitory activity. A set of 846 compounds that inhibited the tubercle bacilli growth were then tested for their ability to bind to MbtA using a fluorescence-ba...
Source: ChemMedChem - August 27, 2019 Category: Chemistry Authors: Ferguson L, Wells G, Bhakta S, Johnson J, Guzman J, Parish T, Prentice RA, Brucoli F Tags: ChemMedChem Source Type: research

Organ-specific dose coefficients derived from Monte Carlo simulations for historical (1930s to 1960s) fluoroscopic and radiographic examinations of tuberculosis patients
This work provides dose coefficients necessary to reconstruct doses used in epidemiological studies of tuberculosis patients treated from the 1930s through the 1960s, who were exposed to diagnostic imaging while undergoing treatment. We made use of averaged imaging parameters from measurement data, physician interviews, and available literature of the Canadian Fluoroscopy Cohort Study and, on occasion, from a similar study of tuberculosis patients from Massachusetts, United States, treated between 1925 and 1954. We used computational phantoms of the human anatomy and Monte Carlo radiation transport methods to compute...
Source: Journal of Radiological Protection - August 27, 2019 Category: Physics Authors: David Borrego, A Iulian Apostoaei, Brian A Thomas, F Owen Hoffman, Steven L Simon, Lydia B Zablotska and Choonsik Lee Source Type: research

[Multidrug-resistant tuberculosis: A management problem that weighs heavily on the University Hospitals of Strasbourg].
CONCLUSIONS: Management of MDR-TB is a real social and medical challenge. Our study shows that the therapeutic protocols used in the management of these patients lead to an unusually high rate of success despite the occurrence of several, sometimes severe, side effects. PMID: 31444025 [PubMed - as supplied by publisher] (Source: Revue des Maladies Respiratoires)
Source: Revue des Maladies Respiratoires - August 26, 2019 Category: Respiratory Medicine Tags: Rev Mal Respir Source Type: research

Assessment of health-related quality of life among tuberculosis patients in a public primary care facility in Indonesia
Conclusion: TB had remarkable negative impacts on patients HRQoL, with physical domain was the most affected. This finding calls upon strategies addressing HRQoL problems in the management of TB patients. (Source: Journal of Global Infectious Diseases)
Source: Journal of Global Infectious Diseases - August 26, 2019 Category: Infectious Diseases Authors: Ika Sartika Widya Norma Insani Rizky Abdulah Source Type: research

Same-Day Tools, Including Xpert Ultra and IRISA-TB, for Rapid Diagnosis of Pleural Tuberculosis: a Prospective Observational Study [Mycobacteriology and Aerobic Actinomycetes]
The diagnosis of pleural tuberculosis (TB) is problematic. The comparative performance of newer same-day tools for pleural TB, including Xpert MTB/RIF Ultra (ULTRA), has hitherto not been comprehensively studied. Adenosine deaminase (ADA), IRISA-TB (interferon gamma ultrasensitive rapid immunosuspension assay), Xpert MTB/RIF, and ULTRA performance outcomes were evaluated in pleural fluid samples from 149 patients with suspected pleural TB. The reference standard was culture positivity (fluid, biopsy specimen, or sputum) and/or pleural biopsy histopathology (termed definite TB). Those designated as having non-TB were negati...
Source: Journal of Clinical Microbiology - August 26, 2019 Category: Microbiology Authors: Meldau, R., Randall, P., Pooran, A., Limberis, J., Makambwa, E., Dhansay, M., Esmail, A., Dheda, K. Tags: Mycobacteriology and Aerobic Actinomycetes Source Type: research

Mycobacterial infections with Ruxolitinib: A retrospective pharmacovigilance review
Patients on Ruxolitinib may be predisposed to mycobacterial infections. This observational, retrospective pharmacovigilance study used the FDA adverse events reporting system to compare the number of patients that developed typical and atypical mycobacterial infections to all drugs in the system. Using the reporting odds ratio, there is an increased risk of developing Tuberculosis (ROR 9.2; 95% CI 7.5-11.4) and atypical mycobacterial infections (ROR 8.3; 95% CI 5.5-12.6) while on treatment with Ruxolitinib. (Source: Clinical Lymphoma, Myeloma and Leukemia)
Source: Clinical Lymphoma, Myeloma and Leukemia - August 26, 2019 Category: Hematology Authors: Kartik Anand, Ethan A. Burns, Joe Ensor, Lawrence Rice, Sai Ravi Pingali Tags: Original Study Source Type: research

Chloroplast-based inducible expression of ESAT-6 antigen for development of a plant-based vaccine against tuberculosis
Publication date: Available online 24 August 2019Source: Journal of BiotechnologyAuthor(s): Kiran Saba, Johanna Gottschamel, Iqra Younus, Tahira Syed, Kehkshan Gull, Andreas Günter Lössl, Bushra Mirza, Mohammad Tahir WaheedAbstractMycobacterium tuberculosis causes tuberculosis in humans. The major disease burden of tuberculosis lies in developing countries. Lack of an effective vaccine for adults is one of the major hurdles for controlling this deadly disease. In the present study, 6 kDa early secretory antigenic target (ESAT-6) of M. tuberculosis was inducibly expressed in chloroplasts of Nicotiana tabacu...
Source: Journal of Biotechnology - August 25, 2019 Category: Biotechnology Source Type: research

Erratum for DeMarco et al., "Determination of [11C]Rifampin Pharmacokinetics within Mycobacterium tuberculosis-Infected Mice by Using Dynamic Positron Emission Tomography Bioimaging".
Erratum for DeMarco et al., "Determination of [11C]Rifampin Pharmacokinetics within Mycobacterium tuberculosis-Infected Mice by Using Dynamic Positron Emission Tomography Bioimaging". Antimicrob Agents Chemother. 2019 Sep;63(9): Authors: DeMarco VP, Ordonez AA, Klunk M, Prideaux B, Wang H, Zhang Z, Tonge PJ, Dannals RF, Holt DP, Lee CKK, Weinstein EA, Dartois V, Dooley KE, Jain SK PMID: 31444171 [PubMed - in process] (Source: Antimicrobial Agents and Chemotherapy)
Source: Antimicrobial Agents and Chemotherapy - August 25, 2019 Category: Microbiology Authors: DeMarco VP, Ordonez AA, Klunk M, Prideaux B, Wang H, Zhang Z, Tonge PJ, Dannals RF, Holt DP, Lee CKK, Weinstein EA, Dartois V, Dooley KE, Jain SK Tags: Antimicrob Agents Chemother Source Type: research

Isolated primary cold abscess of the sternum: a case report
We present an unusual case of a large dumb-bell-shaped cold abscess arising due to infection of the sternum. A 23-year-old immunocompetent Asian woman presented with a gradually progressing painless swelling on anterior chest wall for the last 5  months. She had a large visible swelling on anterior chest wall which was 12.5 cm in diameter, soft, non-tender, temperature was not raised, and fluctuant. Magnetic resonance imaging showed a large dumb-bell-shaped hyperintense collection in upper anterior chest wall with marrow edema and corti cal irregularity in left side of manubrium. Pus was positive for nucleic ...
Source: Journal of Medical Case Reports - August 25, 2019 Category: General Medicine Source Type: research

Three ‐reaction high‐resolution melting assay for rapid differentiation of Mycobacterium tuberculosis complex members
The HRM assay enables rapid identification and differentiation of the clinically most relevant species of MTBC namelyM.  tuberculosis,M.  africanum,M.  microti,M.  bovis,M.  caprae, andM.  bovis BCG from directly extracted samples and cultured material. The use of this powerful assay may save several months of cultivation time and supports improvement of efficient tuberculosis combating strategies worldwide. AbstractThe possibility of introducing a reliable assay for a quick identification and differentiation of the main species ofMycobacterium tuberculosis complex (MTBC) supports the improvem...
Source: MicrobiologyOpen - August 25, 2019 Category: Microbiology Authors: Patricia Landolt, Roger Stephan, Marc J. A. Stevens, Simone Scherrer Tags: ORIGINAL ARTICLE Source Type: research