Photopolymerizable chitosan hydrogels with improved strength and 3D printability
In this study, a novel chiotsan hydrogel is prepared from maleic chitosan (MCS) with high acrylate group substitution (i.e. 1.67) and thiol-terminated poly (ethylene glycol) (TPEG) via step-chain growth photopolymerization approach, which can overcome significantly the oxygen inhibition effect. Rheological property, microstructure, mechanical properties and in vitro degradation can be regulated by changing the thiol/acrylate molar ratio. There is strong intermolecular action between MCS and TPEG. Notably, photopolymerized MCS/TPEG hydrogel exhibited ~2-fold and ~ 10-fold increase in gelling rate and compressive strength, r...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Mengfan Zhang Tingting Wan Penghui Fan Kai Shi Xiao Chen Hongjun Yang Xin Liu Weilin Xu Yingshan Zhou Source Type: research

Load phycocyanin to achieve in vivo imaging of casein-porous starch microgels induced by ultra-high-pressure homogenization
In this study, a novel type of casein-porous starch microgel was prepared under ultra-high-pressure homogenization, by using porous starch with the honeycomb three-dimensional network porous structure. Molecular interaction force analysis and thermodynamic analysis showed that electrostatic interaction played a major role in the formation of microgels. Circular dichroism and Fourier transform infrared spectroscopy showed that homogenization and pH were the main factors, which affected the formation and structural stability of microgels. Compared with casein-glutinous rice starch microgels, the encapsulation efficiency and ...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Dan Hu Zhong Zhang Li Yuan Wenjun Li Yurong Guo Runguang Zhang Xingbin Yang Hailong Peng Source Type: research

Hierarchical crystallization strategy adaptive to 3-dimentional printing of polylactide matrix for complete stereo-complexation
Int J Biol Macromol. 2021 Oct 23:S0141-8130(21)02220-0. doi: 10.1016/j.ijbiomac.2021.10.139. Online ahead of print.ABSTRACTA novel strategy adaptive to 3D printing of PLA matrix for complete stereo-complexation was designed. Stereo-complexation has been demonstrated for its effectiveness in simultaneously improving aqueous stability and heat resistance of PLA. However, current techniques could not be directly incorporated into 3D printing of stereo-complexed PLA since stereo-complexed crystallites are easily formed before printing. High printing temperatures are thus required but decompose PLA materials at the same time. T...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Jing Yang Wei Li Bingnan Mu Helan Xu Xiuliang Hou Yiqi Yang Source Type: research

Mechanism of effect of endogenous/exogenous rice protein and its hydrolysates on rice starch digestibility
Int J Biol Macromol. 2021 Oct 23:S0141-8130(21)02297-2. doi: 10.1016/j.ijbiomac.2021.10.140. Online ahead of print.ABSTRACTThe role of endogenous/exogenous rice protein and its hydrolysates in the enzymatic hydrolysis resistance of rice starch was investigated. Scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and Fourier transform infrared spectroscopy (FTIR) results showed that different types of rice endogenous proteins retarded the digestion of rice starch by the same way. Exogenous addition of protein hydrolysates was more effective than protein for impeding starch digestion. FTIR results i...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Xiaoxue Lu Rongrong Ma Hongwei Qiu Chunrui Sun Yaoqi Tian Source Type: research

Insights into alkaline choline chloride-based deep eutectic solvents pretreatment for Populus deltoides: Lignin structural features and modification mechanism
Int J Biol Macromol. 2021 Oct 23:S0141-8130(21)02292-3. doi: 10.1016/j.ijbiomac.2021.10.134. Online ahead of print.ABSTRACTDeep eutectic solvent (DES) is a kind of green solvent for biorefinery, which favors the progress of being more environmentally friendly and effective. A better understanding of structural changes of lignin is necessary to optimize pretreatment conditions and efficient utilization of the resultant lignin. The current study reported the structural features of lignin recovered from alkaline ChCl/imidazole and ChCl/urea DES pretreatment, and the mechanism of lignin modification was revealed. The profiling...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Haichao Li Xin Li Tingting You Deqiang Li Haq Nawaz Xueming Zhang Feng Xu Source Type: research

Preparation of ribosomal protein S14 polyclonal antibody in broiler pulmonary artery: Its application in broiler ascites syndrome
This study aims to explore the potential role of RPS14 in broiler ascites syndrome (BAS). We successfully prepared polyclonal antibody against RPS14 and studied the localization and expression of RPS14 protein in a variety of animal key tissues. In this experiment, the recombinant expression plasmid PET28a-RPS14 was constructed using the prokaryotic expression technology of foreign genes. Under the conditions of IPTG induction, a His-RPS14 protein with a molecular weight of about 22 kDa was expressed, and the purified recombinant protein was used as an antigen to prepare rabbit anti-chicken serum. Western blot results show...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Yueming Gu Xiaoquan Guo Pei Liu Guyue Li Cheng Huang Fengping Guo Guoliang Hu Cong Wu Zheng Xu Xianling Yang Ping Liu Source Type: research

An antigen display system of GEM nanoparticles based on affinity peptide ligands
Int J Biol Macromol. 2021 Oct 23:S0141-8130(21)02293-5. doi: 10.1016/j.ijbiomac.2021.10.135. Online ahead of print.ABSTRACTGram-positive enhancer matrix (GEM) nanoparticles are often used in mucosal immunity, preparation of subunit vaccines or as an immune adjuvant due to its good immunological activities in recent years. Here, we designed and screened out a high affinity peptide ligand PL23, which could specifically target the non-epitope region of Classic Swine Fever Virus (CSFV) E2 protein, by virtual screening technology, enzyme linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) test. The OD value o...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Man Hu Fangyu Wang Ning Li Guangxu Xing Xuefeng Sun Yunshang Zhang Shuai Cao Ningning Cui Gaiping Zhang Source Type: research

Preparation, characterization and antimicrobial activities of cyclic substituted chitosan derivatives
Int J Biol Macromol. 2021 Oct 23:S0141-8130(21)02259-5. doi: 10.1016/j.ijbiomac.2021.10.101. Online ahead of print.ABSTRACTSix cyclic substituted chitosan derivatives were synthesized, and their structures were characterized by FTIR, 13C NMR and elemental analyses. Additionally, their antimicrobial properties were studied. The synthesized derivatives showed significantly greater zones of inhibition compared with chitosan. The zone of inhibition values produced by C2-2-naphthylamine formamide-C6-2-naphthylamine formyl ester-polymer chitosan against Sarcina sp., Staphylococcus aureus and Escherichia coli were 24 mm, 21 mm an...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Zhenting Zhang Zhimei Zhong Zhihong Zhao Source Type: research

Construction of a CRISPR/FnCas12a multi-sites editing system for inhibiting proliferation of Bombyx mori nuclearpolyhedrosisvirus
Int J Biol Macromol. 2021 Oct 23:S0141-8130(21)02283-2. doi: 10.1016/j.ijbiomac.2021.10.125. Online ahead of print.ABSTRACTIn recent years, Cas12a, a new member of the CRISPR family, has been found to have both DNase and RNase activities, have a simple structure, and a single promoter can simultaneously initiate multiple crRNAs, making the CRISPR/Cas12a editing system more advantageous in terms of structure and mechanism of action. Our team has successfully constructed Cas12a system that can be used in silkworm. Cas12a can be used to edit the multiple target sites. In production, a lot of factors can affect the production ...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Nachuan Liao Zhanqi Dong Xinling Zhang Qi Qin Yan Luo Liang Huang Peng Chen Cheng Lu Minhui Pan Source Type: research

Development of novel hyaluronic acid/human-like collagen bio-composite membranes: A facile "surface modification-assembly" approach
Int J Biol Macromol. 2021 Oct 23:S0141-8130(21)02249-2. doi: 10.1016/j.ijbiomac.2021.10.091. Online ahead of print.ABSTRACTThe merits of hyaluronic acid (HA) as a representative biological carbohydrate polymers especially in bioactivity and tailorability makes it ideal building block for the engineering of tissue engineering scaffolds. HA-based bio-composites integrate the characteristics of multi-component materials, possessing versatility and further improving the therapeutic efficacy. Human like collagen (HLC), which is hydrophilic, biomimetic, and bio-safe, with human tissue-derived collagen biofunction, has attracted ...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Xiaomin Luo Ying Liu Chi Zheng Qianqian Huo Xinhua Liu Source Type: research

Architectural and functional details of CF IA proteins involved in yeast 3'-end pre-mRNA processing and its significance for eukaryotes: A concise review
Int J Biol Macromol. 2021 Oct 23:S0141-8130(21)02287-X. doi: 10.1016/j.ijbiomac.2021.10.129. Online ahead of print.ABSTRACTIn eukaryotes, maturation of pre-mRNA relies on its precise 3'-end processing. This processing involves co-transcriptional steps regulated by sequence elements and other proteins. Although, it holds tremendous importance, defect in the processing machinery will result in erroneous pre-mRNA maturation leading to defective translation. Remarkably, more than 20 proteins in humans and yeast share homology and execute this processing. The defects in this processing are associated with various diseases in hu...
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Mandeep Kaur Akanksha Sharma Gurpal Singh Santosh Kumar Ravi P Barnwal Source Type: research

A review of plant-based expression systems as a platform for single-domain recombinant antibody production
This article presents the main advances associated to the heterologous expression of VHHs in plant systems. Besides, we show insights related to the use of VHHs as a strategy for plant pathogen control and a tool for genomic manipulation in plant systems.PMID:34699899 | DOI:10.1016/j.ijbiomac.2021.10.126 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Angela Donato Maia Malaquias L ívia Erika Carlos Marques Soraya S Pereira Cleberson de Freitas Fernandes Andrea Queiroz Maranh ão Rodrigo G Stabeli Eridan Orlando Pereira Tramontina Florean Maria Izabel Florindo Guedes Carla Freire Celedonio Fernandes Source Type: research

Antisolvent versus ultrasonication: Bottom-up and top-down approaches to produce lignin nanoparticles (LNPs) with tailored properties
This study showed that interesting properties of LNPs can be easily tailored and combined focusing on the various applications of these versatile nanostructures. In a model application, different types of LNPs were incorporated into poly(vinyl alcohol)-based nanocomposites, modulating the UV-protection capability of the polymer matrix.PMID:34699900 | DOI:10.1016/j.ijbiomac.2021.10.094 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Camilla H M Camargos Camila A Rezende Source Type: research

Recent advancements in synthesis and drug delivery utilization of polysaccharides-based nanocomposites: The important role of nanoparticles and layered double hydroxides
Int J Biol Macromol. 2021 Oct 22:S0141-8130(21)02281-9. doi: 10.1016/j.ijbiomac.2021.10.123. Online ahead of print.ABSTRACTDrug delivery systems are explained as methods to deliver a specific drug to desired organs, tissues, and cells for drug release to diseases treatment. Recently, considerable development has been interested in stimuli-responsive nano-systems, which respond to the essential pathological and physicochemical issues in diseased sites. During the last decades, researchers in the world presented, investigated, and implemented novel different nanomaterials with a focus on developing drug delivery. Polysacchar...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Shadpour Mallakpour Elham Azadi Chaudhery Mustansar Hussain Source Type: research

Improving the flame retardancy and accelerating the degradation of poly (lactic acid) in soil by introducing fully bio-based additives
In this study, a novel bio-based flame retardant LC-PA is prepared by the Mannich reaction between phytic acid (PA) and L-citrulline (LC). LC-PA is combined with tannic acid (TA) and introduced into PLA to improve fire performance and accelerate biodegradability. Compared with control PLA, the PLA composite containing 10% LC-PA/TA increases the LOI value to 26.9%, reaches a V-0 rating in the UL-94 test, and reduces the peak heat release rate and total heat release by 24.5% and 21.1%, respectively. More importantly, the introduction of LC-PA/TA accelerates the degradation rate of PLA in soil, which is of significance for bi...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Shuang Qiu Yuchun Li Peng Qi Dan Meng Jun Sun Hongfei Li Zheng Cui Xiaoyu Gu Sheng Zhang Source Type: research

Nanocomposite base on carboxymethylcellulose hydrogel: Simultaneous absorbent of ethylene and humidity to increase the shelf life of banana fruit
Int J Biol Macromol. 2021 Oct 22:S0141-8130(21)02225-X. doi: 10.1016/j.ijbiomac.2021.10.075. Online ahead of print.ABSTRACTThe main purpose of this study was to increase shelf life of banana using active hydrogel. For this purpose, carboxymethylcellulose/nanofiber cellulose/potassium permanganate (CMC/NFC/KMnO4) hydrogel film was prepared. The morphology and physicochemical properties of CMC hydrogels was investigated. The prepared films were used as humidity/ethylene absorbent in banana packaging for 30 days at 0 and 25 °C. The physical, mechanical and sensory properties of bananas were studied during storage. SEM ima...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Sajad Pirsa Source Type: research

Lignin-based carbon nanofibers: Morphologies, properties, and features as substrates for pseudocapacitor electrodes
Int J Biol Macromol. 2021 Oct 22:S0141-8130(21)02266-2. doi: 10.1016/j.ijbiomac.2021.10.108. Online ahead of print.ABSTRACTIn this work, lignin-based carbon nanofibers (LCNFs) were for the first time served as substrate for in-situ electrodeposition of polyaniline (PANI) and tested as pseudocapacitor. Two LCNFs with different lignin ratios were designed to distinguish their morphology and structural properties. Next, PANI deposition mechanisms on both LCNFs were investigated and the electrochemical performance of the resulting LCNF/PANIs were evaluated. It was found although LCNF2 was composed of less uniform nanofibers du...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Pengyu Hu Hong Jin Keliang Wang Zizhu Zhao Wangda Qu Source Type: research

Influence of cellulose nanocrystal addition on the production and characterization of bacterial nanocellulose
This study investigated the effect of adding different concentrations (0%, 0.5%, and 1.0%) of cellulose nanocrystal (CNC) in Hestrin-Schramm modified medium on the yield and properties of BNC produced by Komagataeibacter sp. SFCB22-18. The BNC yield increased as following an increase in added CNC concentration. Also, the morphology, structure, crystallinity, thermal stability, and mechanical properties of BNC improved after CNC incorporation. A low CNC concentration (0.1%) favored mechanical strength, whereas 0.5% gave the optimum morphology, structural, and thermal stability. These results showed that modifying BNC with C...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Won Yeong Bang Olajide Emmanuel Adedeji Hye Jee Kang Mi Dan Kang Jungwoo Yang Young Woon Lim Young Hoon Jung Source Type: research

Controllable conversion of shrimp shells into chitin or derived carbon material using acidic deep eutectic solvent
Int J Biol Macromol. 2021 Oct 22:S0141-8130(21)02279-0. doi: 10.1016/j.ijbiomac.2021.10.122. Online ahead of print.ABSTRACTIt is important to convert waste shrimp shells into chitin and its derivatives, but it still faces disadvantages of pollution and long process. In this research, deep eutectic solvents made from choline chloride (ChCl) and p-toluenesulfonic acid monohydrate (TsOH) were used to convert shrimp shells into chitin or carbon materials through adjustment of temperature with a dissolution-regeneration method in one step. Obtained precipitates and regenerated products gradually changed from chitin to carbon ma...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Mi Feng Jipeng Yan Bin He Xinyan Chen Jian Sun Source Type: research

Recent advancements in synthesis and drug delivery utilization of polysaccharides-based nanocomposites: The important role of nanoparticles and layered double hydroxides
Int J Biol Macromol. 2021 Oct 22:S0141-8130(21)02281-9. doi: 10.1016/j.ijbiomac.2021.10.123. Online ahead of print.ABSTRACTDrug delivery systems are explained as methods to deliver a specific drug to desired organs, tissues, and cells for drug release to diseases treatment. Recently, considerable development has been interested in stimuli-responsive nano-systems, which respond to the essential pathological and physicochemical issues in diseased sites. During the last decades, researchers in the world presented, investigated, and implemented novel different nanomaterials with a focus on developing drug delivery. Polysacchar...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Shadpour Mallakpour Elham Azadi Chaudhery Mustansar Hussain Source Type: research

Improving the flame retardancy and accelerating the degradation of poly (lactic acid) in soil by introducing fully bio-based additives
In this study, a novel bio-based flame retardant LC-PA is prepared by the Mannich reaction between phytic acid (PA) and L-citrulline (LC). LC-PA is combined with tannic acid (TA) and introduced into PLA to improve fire performance and accelerate biodegradability. Compared with control PLA, the PLA composite containing 10% LC-PA/TA increases the LOI value to 26.9%, reaches a V-0 rating in the UL-94 test, and reduces the peak heat release rate and total heat release by 24.5% and 21.1%, respectively. More importantly, the introduction of LC-PA/TA accelerates the degradation rate of PLA in soil, which is of significance for bi...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Shuang Qiu Yuchun Li Peng Qi Dan Meng Jun Sun Hongfei Li Zheng Cui Xiaoyu Gu Sheng Zhang Source Type: research

Nanocomposite base on carboxymethylcellulose hydrogel: Simultaneous absorbent of ethylene and humidity to increase the shelf life of banana fruit
Int J Biol Macromol. 2021 Oct 22:S0141-8130(21)02225-X. doi: 10.1016/j.ijbiomac.2021.10.075. Online ahead of print.ABSTRACTThe main purpose of this study was to increase shelf life of banana using active hydrogel. For this purpose, carboxymethylcellulose/nanofiber cellulose/potassium permanganate (CMC/NFC/KMnO4) hydrogel film was prepared. The morphology and physicochemical properties of CMC hydrogels was investigated. The prepared films were used as humidity/ethylene absorbent in banana packaging for 30 days at 0 and 25 °C. The physical, mechanical and sensory properties of bananas were studied during storage. SEM ima...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Sajad Pirsa Source Type: research

Lignin-based carbon nanofibers: Morphologies, properties, and features as substrates for pseudocapacitor electrodes
Int J Biol Macromol. 2021 Oct 22:S0141-8130(21)02266-2. doi: 10.1016/j.ijbiomac.2021.10.108. Online ahead of print.ABSTRACTIn this work, lignin-based carbon nanofibers (LCNFs) were for the first time served as substrate for in-situ electrodeposition of polyaniline (PANI) and tested as pseudocapacitor. Two LCNFs with different lignin ratios were designed to distinguish their morphology and structural properties. Next, PANI deposition mechanisms on both LCNFs were investigated and the electrochemical performance of the resulting LCNF/PANIs were evaluated. It was found although LCNF2 was composed of less uniform nanofibers du...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Pengyu Hu Hong Jin Keliang Wang Zizhu Zhao Wangda Qu Source Type: research

Influence of cellulose nanocrystal addition on the production and characterization of bacterial nanocellulose
This study investigated the effect of adding different concentrations (0%, 0.5%, and 1.0%) of cellulose nanocrystal (CNC) in Hestrin-Schramm modified medium on the yield and properties of BNC produced by Komagataeibacter sp. SFCB22-18. The BNC yield increased as following an increase in added CNC concentration. Also, the morphology, structure, crystallinity, thermal stability, and mechanical properties of BNC improved after CNC incorporation. A low CNC concentration (0.1%) favored mechanical strength, whereas 0.5% gave the optimum morphology, structural, and thermal stability. These results showed that modifying BNC with C...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Won Yeong Bang Olajide Emmanuel Adedeji Hye Jee Kang Mi Dan Kang Jungwoo Yang Young Woon Lim Young Hoon Jung Source Type: research

Controllable conversion of shrimp shells into chitin or derived carbon material using acidic deep eutectic solvent
Int J Biol Macromol. 2021 Oct 22:S0141-8130(21)02279-0. doi: 10.1016/j.ijbiomac.2021.10.122. Online ahead of print.ABSTRACTIt is important to convert waste shrimp shells into chitin and its derivatives, but it still faces disadvantages of pollution and long process. In this research, deep eutectic solvents made from choline chloride (ChCl) and p-toluenesulfonic acid monohydrate (TsOH) were used to convert shrimp shells into chitin or carbon materials through adjustment of temperature with a dissolution-regeneration method in one step. Obtained precipitates and regenerated products gradually changed from chitin to carbon ma...
Source: International Journal of Biological Macromolecules - October 25, 2021 Category: Biochemistry Authors: Mi Feng Jipeng Yan Bin He Xinyan Chen Jian Sun Source Type: research

Combining carvacrol and nisin in biodegradable films for antibacterial packaging applications
Int J Biol Macromol. 2021 Oct 21:S0141-8130(21)02276-5. doi: 10.1016/j.ijbiomac.2021.10.118. Online ahead of print.ABSTRACTIn this work, the feasibility of antibacterial biopolymeric films containing carvacrol (CRV) and a nisin commercial formulation (Nis) for potential food packaging applications was investigated. As polymer matrix, a commercial biodegradable polymer formulation of Mater-Bi (MB) was chosen due to its significant food packaging applications. CRV and Nis were chosen due to their well-established antibacterial properties and their potential synergistic effect. MB/CRV, MB/Nis, and MB/CRV/Nis systems were prod...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Francesco Lopresti Luigi Botta Vincenzo La Carrubba Liliana Di Pasquale Luca Settanni Raimondo Gaglio Source Type: research

Class I hydrophobin fusion with cellulose binding domain for its soluble expression and facile purification
Int J Biol Macromol. 2021 Oct 21;193(Pt A):38-43. doi: 10.1016/j.ijbiomac.2021.10.089. Online ahead of print.ABSTRACTHydrophobins, highly surface-active proteins, have the ability to reverse surface hydrophobicity through self-assembly at the hydrophilic-hydrophobic interfaces. Their unique structure and interfacial activity lead hydrophobins to have potential applications on surface functional modifications. However, class I hydrophobins are prone to self-assemble into highly insoluble amyloid-like rodlets structure. Recombinant hydrophobins could be produced by Escherichia coli but generally as an insoluble inclusion bod...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Nathania Puspitasari Cheng-Kang Lee Source Type: research

Effect of Ca < sup > 2+ < /sup > cross-linking on the properties and structure of lutein-loaded sodium alginate hydrogels
Int J Biol Macromol. 2021 Oct 21:S0141-8130(21)02272-8. doi: 10.1016/j.ijbiomac.2021.10.114. Online ahead of print.ABSTRACTIn order to construct nano-lutein hydrogels with sustained release properties, the basic properties and structure of nano-lutein hydrogels cross-linked with different concentrations of Ca2+ were investigated. The results showed that the highest loading capacity for lutein reached 770.88 μg/g, while the encapsulation efficiency was as high as 99.39%. When Ca2+ concentration was lower than 7.5 mM, the filling of lutein nanoparticles reduced the hardness and gumminess of the hydrogel. The resilience an...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Pengxiang Xu Jiangfeng Song Zhuqing Dai Yayuan Xu Dajing Li Caie Wu Source Type: research

Solid acid facilitated deep eutectic solvents extraction of high-purity and antioxidative lignin production from poplar wood
In this study, two kinds of different promising solid acid (SA) with DESs were designed to promote the pretreatment of lignocellulose. The SA of phosphotungstic acid (H3O40PW12) and iron bromide (FeBr3) were introduced into DESs to extract poplar wood lignin and evaluate the antioxidant activity. It was found that 82.2% and 80.9% of lignin were obtained from poplar wood under H3O40PW12-ChCl/Lac acid and FeBr3-choline ChCl/Lac system with 4 h and 8 h, respectively. The lignin fractions with a high purity (>89%), low molecular weight (Mw 1800-2000 g/mol). Besides, the antioxidant activities of lignin fractions were better...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Yinglong Wu Jinru Cheng Qian Yang Jianquan Hu Quanwei Zhou Lingyuan Wang Zhong Liu Lanfeng Hui Source Type: research

Adsorption of methylene blue dye on sodium alginate/polypyrrole nanotube composites
This study the performance of polypyrrole nanotube incorporated sodium alginate (SA/PPyNT) on the adsorptive removal of methylene blue dye from an aqueous solution was investigated. Firstly, polypyrrole nanotubes were synthesized by oxidative chemical polymerization. Then, polypyrrole nanotubes were added to the sodium alginate gel and the composite beads were prepared by a crosslinking process in a 3% CaCl2 solution. The composite beads were characterized using Fourier transform infrared-attenuated total reflectance (FTIR-ATR), scanning electron microscope (SEM), and atomic force microscopy analyzes. In the adsorption stu...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Ezgi Nur Heybet Volkan Ugraskan Birol Isik Ozlem Yazici Source Type: research

Structural characterization of a water-soluble polysaccharide from Angelica dahurica and its antitumor activity in H22 tumor-bearing mice
Int J Biol Macromol. 2021 Oct 21:S0141-8130(21)02268-6. doi: 10.1016/j.ijbiomac.2021.10.110. Online ahead of print.ABSTRACTA novel Angelica dahurica polysaccharide (ADP) with Mw of 6.09 × 103 Da was isolated. The contents of total sugar and uronic acid in ADP were 91.04% and 12.69%. The structure characteristics indicated that ADP was an acidic polysaccharide consisting of rhamnose, arabinose, galactose, glucose, mannose, glucuronic acid and galacturonic acid (0.09: 0.61: 1.88: 1: 0.14: 0.63: 0.03). Moreover, there were →3)-Manp-(1→, →4, 6)-Galp-(1→, →4)-Galp-(1→, →3)-Glcp-(1→,...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Xiao-Dan Dong Yi-Ning Liu Yan Zhao An-Jun Liu Hai-Yu Ji Juan Yu Source Type: research

Dual-responsive nisin loaded chondroitin sulfate nanogel for treatment of bacterial infection in soft tissues
Int J Biol Macromol. 2021 Oct 21:S0141-8130(21)02274-1. doi: 10.1016/j.ijbiomac.2021.10.116. Online ahead of print.ABSTRACTChondroitin sulfate-Nisin nanogels (CS-N NGs) were prepared by electrostatic interaction for nisin delivery as an antibacterial agent in the treatment of bacterial infections caused by some clinical strains of methicillin-resistant and methicillin-sensitive Staphylococcus aureus (S. aureus). The physical properties of CS-N NGs were evaluated using Fourier-transform infrared spectroscopy, dynamic light scattering, and field emission scanning electron microscopy. The average diameter of obtained CS-N NGs...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Mohsen Tayeferad Soheil Boddohi Bita Bakhshi Source Type: research

Synthesis of chitosan/silver nanocomposites by phase inversion with the assistance of carbon dioxide
Int J Biol Macromol. 2021 Oct 21:S0141-8130(21)02273-X. doi: 10.1016/j.ijbiomac.2021.10.115. Online ahead of print.ABSTRACTCarbon dioxide (CO2) assisted synthesis of water-soluble silver nanoparticle with a narrow particle size distribution is reported here based on the phase-inversion procedure. Bio-derived chitosan (CS) is used to stabilize the metal nanoparticles according to its abundant functional groups. Formic acid is employed as both a solvent (for the polymer) and a reductant for in-situ reducing the silver precursor along with the solvent evaporation. CO2 is utilized to combine with the amino groups of CS, reduci...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Binqing Zhao Qi Zhou Chenxi Lou Xinpeng Jin Wei Li Source Type: research

Synthesis of Eudragit ® L100-coated chitosan-based nanoparticles for oral enoxaparin delivery
In this study, we developed an Eudragit® L100 coated chitosan core shell nanoparticles for enoxaparin oral delivery (Eud/CS/Enox NPs) through a completely eco-friendly method without employing any high-energy homogenizer technique and any organic solvents. Spherical nanocarriers were successfully prepared with particle size lower than 300 nm, polydispersity index about 0.12 and zeta potential higher than +25 mV, entrapment efficiency greater than 95% and the in vitro release behavior confirms the good colloidal stability and the successful Eudragit® L100 coating process demonstrated by negligible cumulative enoxaparin release (
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Yuri Basilio Gomes Patriota Igor Eduardo Silva Arruda Ant ônia Carla de Jesus Oliveira Thaisa Cardoso de Oliveira Eliadna de Lemos Vasconcelos Silva Lu íse Lopes Chaves F ábio de Oliveira Silva Ribeiro Durcilene Alves da Silva M ônica Felts de La Roca Source Type: research

Identification and the immunological role of two Nimrod family genes in the silkworm, Bombyx mori
Int J Biol Macromol. 2021 Oct 21:S0141-8130(21)02240-6. doi: 10.1016/j.ijbiomac.2021.10.083. Online ahead of print.ABSTRACTIn animals, immune signaling pathways and effector molecules participate in attenuating microbial infection. Recent work has shown that the Nimrod family proteins can directly bind to bacteria, and this binding leads to bacterial phagocytosis. Although the Nimrod gene family has been reported in many non-drosophilids, their functions remain unexplored in most insect species. Here, we report two members (Nimrod-B and Draper) of the Nimrod gene family from Bombyx mori and analyzed their role in immunity....
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Isma Gul Saima Kausar Qiuxiang You Wei Sun Zekun Li Muhammad Nadeem Abbas Hongjuan Cui Source Type: research

Composition and structure elucidation of bovine milk glycosaminoglycans and their anti-adipogenic activity via modulation PPAR- γ and C/EBP-α
Int J Biol Macromol. 2021 Oct 21:S0141-8130(21)02229-7. doi: 10.1016/j.ijbiomac.2021.10.076. Online ahead of print.ABSTRACTThe structure function relation of Glycosaminoglycans from bovine milk (bmGAGs) has not been studied in detail. In the present study bmGAGs was isolated and structurally characterized. Chondroitin sulphate was one of the major GAGs present and had 65% of ΔDi-diSB (GlcA(2S)-GalNAc(4S)), followed by 18% of ΔDi-4S(Δ4,5HexUAα1 → 3GalNAc). Further, bmGAGs exhibited a marked anti-adipogenic effect in 3 T3-L1 cells without affecting cell viability at the concentration used. The tr...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Chaitra Rai C D Nandini C G Poornima Priyadarshini Source Type: research

Erythrocyte membrane-camouflaged gefitinib/albumin nanoparticles for tumor imaging and targeted therapy against lung cancer
Int J Biol Macromol. 2021 Oct 21:S0141-8130(21)02271-6. doi: 10.1016/j.ijbiomac.2021.10.113. Online ahead of print.ABSTRACTConventional chemotherapeutic drugs may cause serious side effects such as hepatotoxicity and renal toxicity due to lack of targeting, which affects therapy outcome and the prognosis of patients. Therefore, biomimetic nanoparticles with long blood circulation and active targeting have attracted increasing attention. In this work, we fabricated a biomimetic R-RBC@GEF-NPs nano-system by encapsulating gefitinib-loaded albumin nanoparticles (GEF-NPs) inside cRGD-modified red blood cell (RBC) membranes. The...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Qian Wen Yan Zhang Tobias Achu Muluh Kang Xiong BiQiong Wang Yun Lu ZhouXue Wu YanLin Liu Huan Shi SuSu Xiao ShaoZhi Fu Source Type: research

Structural characterization of polysaccharide purified from Hericium erinaceus fermented mycelium and its pharmacological basis for application in Alzheimer's disease: Oxidative stress related calcium homeostasis
Int J Biol Macromol. 2021 Oct 21:S0141-8130(21)02275-3. doi: 10.1016/j.ijbiomac.2021.10.117. Online ahead of print.ABSTRACTThe purified polysaccharides from Hericium erinaceus fermented mycelium entitled with PHEB was analyzed and it was mainly composed of six glycosidic bonds. It has been confirmed to show the relieving activity against Alzheimer's Disease (AD)- just as behaviors of B6C3-Tg (APPswePSEN1d E9)/Nju double transgenic [Genotype: (Appswe)T, (Psen1) T] (APP/PS1) mice. Six-week PHEB administration significantly improved the cognitive and anxious behaviors of mice. Brain injury, amyloid beta deposition and tau hyp...
Source: International Journal of Biological Macromolecules - October 24, 2021 Category: Biochemistry Authors: Wenji Hu Minkai Song Chunyue Wang Ziang Guo Yu Li Di Wang Source Type: research

Hydroxylated single-walled carbon nanotube inhibits β2m < sub > 21 < /sub > < sub > - < /sub > < sub > 31 < /sub > fibrillization and disrupts pre-formed proto-fibrils
Int J Biol Macromol. 2021 Oct 20;193(Pt A):1-7. doi: 10.1016/j.ijbiomac.2021.10.103. Online ahead of print.ABSTRACTPathological aggregation of amyloid polypeptides is associated with numerous degenerative diseases. Preventing aggregation and clearing amyloid deposits are considered as promising strategies against amyloidosis. With the capacity of crossing the blood-brain barrier and good biocompatibility, the hydroxylated single-walled carbon nanotube (SWCNT-OH) has been shown with excellent anti-amyloid properties. Here, we systematically studied the SWCNT-OH effects on the fibrillization of the β2m21-31 peptides uti...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: Yu Zhang Yuying Liu Wenhui Zhao Yunxiang Sun Source Type: research

Vanillin chitosan miscible hydrogel blends and their prospects for 3D printing biomedical applications
Int J Biol Macromol. 2021 Oct 20:S0141-8130(21)02251-0. doi: 10.1016/j.ijbiomac.2021.10.093. Online ahead of print.ABSTRACTIn the present study, chitosan (CS) reacted with vanillin through a Schiff base reaction forming the vanillin-CS (VACS) derivative. FTIR and 1H NMR spectra confirmed the derivatization of CS, the enhanced swelling behavior was long-established while XRD measurement stated the semicrystalline nature of the VACS derivative. In a further step, blends between CS and VACS were prepared in ratios CS/VACS 90/10 up to 10/90 w/w and the formation of hydrogen bonds was noticed through FTIR and XRD measurements. ...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: Georgia Michailidou Emmanuel N Koukaras Dimitrios N Bikiaris Source Type: research

Lipase immobilization on a novel class of Zr-MOF/electrospun nanofibrous polymers: Biochemical characterization and efficient biodiesel production
In this study, due to the favorable properties of MOF compounds and fibrous materials, new nanostructures of Zr-MOF/PVP nanofibrous composites were synthesized by electrospinning procedure. The related features of these samples were characterized by relevant analyzes, including SEM, BET surface area analysis, XRD, and FTIR spectroscopy. The final product showed significant properties such as small particle size distribution, large surface area, and high crystallinity. This strategy for producing these nanostructures could lead to new compounds as novel alternative materials for biological applications. Lipase MG10 was succ...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: Arastoo Badoei-Dalfard Arezoo Shahba Fatemeh Zare Ghasem Sargazi Bagher Seyedalipour Zahra Karami Source Type: research

New findings on GFP-like protein application as fluorescent tags: Fibrillogenesis, oligomerization, and amorphous aggregation
Int J Biol Macromol. 2021 Oct 20:S0141-8130(21)02265-0. doi: 10.1016/j.ijbiomac.2021.10.107. Online ahead of print.ABSTRACTGreen fluorescent proteins (GFP) are commonly used as fluorescent tags and biosensors in cell biology and medicine. However, the propensity of GFP-like proteins to aggregate and the consequence of intermolecular interaction for their application have not been thoroughly examined. In this work, alternative aggregation pathways of superfolder green fluorescent protein (sfGFP) were demonstrated using a spectroscopic and microscopic study of the samples prepared by equilibrium microdialysis. Besides oligom...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: Olga V Stepanenko Maksim I Sulatsky Ekaterina V Mikhailova Irina M Kuznetsova Konstantin K Turoverov Olesya V Stepanenko Anna I Sulatskaya Source Type: research

Fluoxetine and sertraline based multitarget inhibitors of cholinesterases and monoamine oxidase-A/B for the treatment of Alzheimer's disease: Synthesis, pharmacology and molecular modeling studies
Int J Biol Macromol. 2021 Oct 20;193(Pt A):19-26. doi: 10.1016/j.ijbiomac.2021.10.102. Online ahead of print.ABSTRACTFor the potential therapy of Alzheimer's disease (AD), cholinesterases (ChE) and monoamine oxidase (MAO) are key enzymes that regulate the level of acetylcholinesterase (AChE)/butyrylcholinesterase (BChE) and monoamines. The aim of current research is the synthesis of multi-target compounds that can concomitantly inhibit ChEs and MAO. A series of fluoxetine and sertraline hybrids was designed and evaluated as multi-target inhibitors of ChEs and hMAO. In-vitro enzyme inhibition studies demonstrated that a num...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: Muhammad Shahid Nadeem Jalaluddin Azam Khan Umer Rashid Source Type: research

Characterization of potential cellulose fiber from cattail fiber: A study on micro/nano structure and other properties
In this study, the physical and chemical properties, micro/nano structure, and mechanical characteristics of CFs were investigated. The CFs have a low density (618.0 kg m-3). The results of transmission electron microscopy and tensile testing data indicated that the cattail trunk fiber (CTF) bundle is composed of parenchyma cells and solid stone cells, demonstrating high specific modulus (10.1 MPa∙m3·kg-1) and high elongation at break (3.9%). In turn, the cattail branch fiber (CBF) bundle is composed of parenchyma cells with specific "half-honeycomb" shape. The inner diaphragms divide these cells into th...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: Shanshan Wu Jinlong Zhang Chuangye Li Fuli Wang Lanlan Shi Mengxue Tao Beibei Weng Bin Yan Yong Guo Yuxia Chen Source Type: research

Aligned silk fibroin/poly-3-hydroxybutyrate nanofibrous scaffolds seeded with adipose-derived stem cells for tendon tissue engineering
Int J Biol Macromol. 2021 Oct 20:S0141-8130(21)02262-5. doi: 10.1016/j.ijbiomac.2021.10.104. Online ahead of print.ABSTRACTIn this work we investigated tenogenic differentiation of adipose-derived mesenchymal stem cells (AdMSCs), which were seeded onto silk fibroin/poly-3-hydroxybutyrate (SF/P3HB) scaffolds with aligned topography, and high mechanical strength. The electrospinning process was optimized by using the response surface method (RSM) and SF/P3HB nanofibrous matrices with a total polymer concentration of 5% (SF: PHB = 3: 1), flow rate 1 mL/h, collector rotation speed 2000 rpm, applied voltage 14 kV, and collector...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: Burcu Sar ıkaya Menem şe Gümüşderelioğlu Source Type: research

Genome-wide characterization and expression analysis of cystathionine β-synthase genes in plant development and abiotic stresses of cotton (Gossypium spp.)
Int J Biol Macromol. 2021 Oct 20:S0141-8130(21)02231-5. doi: 10.1016/j.ijbiomac.2021.10.079. Online ahead of print.ABSTRACTCystathionine β-synthase (CBS) domains containing proteins (CDCPs) form a large family and play roles in development via regulation of the thioredoxin system as well as abiotic and biotic stress responses of plant. However, the comprehensive study of CBS genes remained elusive in cotton. Here, we identified 237 CBS genes in 11 plant species and the phylogenetic analysis categorized CBS genes into four groups. Whole-genome or segmental with dispersed duplication events contributed to GhCBS gene fam...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: Faiza Ali Yonghui Li Fuguang Li Zhi Wang Source Type: research

TGF- β1-stimulation of NFATC2 and ATF3 proteins and their interaction for matrix metalloproteinase 13 expression in human breast cancer cells
This study found that TGF-β1 stimulated the expression of the nuclear factor of activated T Cells 2 (NFATC2) in MDA-MB231 cells and provided evidence of its interaction with ATF3. The functional characterization of NFATC2 in association with ATF3 was determined by silencing of NFATC2 using siRNA. Knock-down of NFATC2 decreased the expression of both ATF3 and its target gene MMP13 (matrix metalloproteinase 13, a critical invasive gene) in hBC cells. Chromatin immunoprecipitation revealed that TGF-β1 promoted NFATC2 binding and NFATC2-ATF3 complex binding at the MMP13 promoter region, whereas silencing of NFATC2 de...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: M Rohini M Vairamani N Selvamurugan Source Type: research

Self-assembly of collagen fibrils on graphene oxide and their hybrid nanocomposite films
Int J Biol Macromol. 2021 Oct 20:S0141-8130(21)02256-X. doi: 10.1016/j.ijbiomac.2021.10.098. Online ahead of print.ABSTRACTIn order to explore the distribution, conformation and interaction of collagen on GO nanosheet surfaces, the mechanism of self-assembly of collagen was investigated in the presence of GO nanosheets. Atomic force microscopy (AFM) was employed to observe the conformation of self-assembled collagen fibrils on the GO nanosheets surfaces. The collagen concentration and incubation time mainly affect the size of the collagen fibrils while the pH of the dispersion determines the self-assembly sites of collagen...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: Chengfei Yue Changkun Ding Xuan Du Yanjie Wang Jieliang Su Bowen Cheng Source Type: research

Structure of dye-decolorizing peroxidase from Bacillus subtilis in complex with veratryl alcohol
In this study, the crystal structure of BsDyP- veratryl alcohol (VA) complex delves deep into the binding of small substrate molecules within the DyP heme cavity. The biochemical analysis shows that BsDyP oxidizes the VA with a turnover number of 0.065 s-1, followed by the oxidation of 2,6-dimethoxyphenol (DMP) and guaiacol with a comparable turnover number (kcat) of 0.07 s-1 and 0.07 s-1, respectively. Moreover, biophysical and computational studies reveal the comparable binding affinity of substrates to BsDyP and produce lower-energy stable BsDyP-ligand(s) complexes. All together with our previous findings, we are provid...
Source: International Journal of Biological Macromolecules - October 23, 2021 Category: Biochemistry Authors: Poonam Dhankhar Vikram Dalal Vishakha Singh Ashwani Kumar Sharma Pravindra Kumar Source Type: research

Fabrication and characterization of an economical active packaging film based on chitosan incorporated with pomegranate peel
Int J Biol Macromol. 2021 Oct 19:S0141-8130(21)02217-0. doi: 10.1016/j.ijbiomac.2021.10.064. Online ahead of print.ABSTRACTAntioxidant and antimicrobial chitosan (CS) films incorporated with different concentrations (0, 3, 6 and 9% w/w based on chitosan) of pomegranate peel powder (PPP) were prepared through a simple and low-cost process and characterized. The physicochemical property, antioxidant and antibacterial properties of the films were investigated. Results showed that incorporation with PPP increased the thickness, water solubility (WS), water vapor permeability (WVP), opacity and total phenolic content (TPC) of c...
Source: International Journal of Biological Macromolecules - October 22, 2021 Category: Biochemistry Authors: Jun Zeng Xiaona Ren Siming Zhu Youcheng Gao Source Type: research