Rational design of a Yarrowia lipolytica derived lipase for improved thermostability.
Abstract To improve the thermostability of the lipase LIP2 from Yarrowia lipolytica, molecular dynamics (MD) simulations at various temperatures were used to investigate the common fluctuation sites of the protein, which are considered to be thermally weak points. Two of these residues were selected for mutations to improve the enzyme's thermostability, and the variants predicted by MD simulations to have improved thermostability were expressed in Pichia pastoris GS115 for further investigations. According to the proline rule, the high fluctuation site S115 or V213 was replaced with proline residue, the two lipase...
Source: International Journal of Biological Macromolecules - July 10, 2019 Category: Biochemistry Authors: Zhang H, Sang J, Zhang Y, Sun T, Liu H, Yue R, Zhang J, Wang H, Dai Y, Lu F, Liu F Tags: Int J Biol Macromol Source Type: research

Effect of hydroxypropyl methylcellulose molecular weight on supramolecular structures and properties of HPMC/sodium citrate photophobic films.
Abstract Photophobic (white) films were prepared by mixing hydroxypropyl methylcellulose (HPMC) of different molecular weights with sodium citrate (SC). Wide angle X-ray diffractometry, small angle X-ray scattering, scanning electron microscopy, dynamic mechanical analysis, whiteness determination and texture analysis were used to investigate the effect of HPMC molecular weight on the aggregation structures and properties of HPMC film. The crystalline integrity decreased with the increased molecular weight of HPMC for these HPMC/SC films. Similar crystallinity was observed for 6HPMC/SC and 15HPMC/SC, which was lar...
Source: International Journal of Biological Macromolecules - July 9, 2019 Category: Biochemistry Authors: Zhang L, Lu YQ, Yu YX, Li Q, Qian JY, He XL Tags: Int J Biol Macromol Source Type: research

Roles of T875N somatic mutation in the activity, structural stability of JAK2 and the transformation of OCI-AML3 cells.
This study provides clues in understanding structural basis of T875N mutation caused JAK2 hyperactivation and its roles in the pathology of AML. PMID: 31299252 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 9, 2019 Category: Biochemistry Authors: Chen C, Li F, Ma MM, Zhang S, Liu Y, Yan ZL, Chen W, Cao J, Zeng LY, Wang XY, Xu KL, Wu QY Tags: Int J Biol Macromol Source Type: research

A synergistic analysis of drag reduction on binary polymer mixtures containing guar gum.
Abstract Drag reduction by the addition of polymer additives has been widely studied. However, there are only a few studies on binary polymer mixtures, here named blends. In this work, xanthan gum, polyacrylamide and poly(ethylene oxide) were associated with guar gum and drag reduction was used as a parameter to determine the synergistic interaction between polymers. The aim was to verify the relation of the synergy with the rigidity of the polymeric chains, the molecular weights and the magnitude of the molecular interactions between the studied polymers. To that end, several ratios of mixtures were tested at dif...
Source: International Journal of Biological Macromolecules - July 9, 2019 Category: Biochemistry Authors: Novelli GL, Ferrari LA, Vargas GG, Loureiro BV Tags: Int J Biol Macromol Source Type: research

Sub-micron sized cytochrome c particles adsorbing to solid surfaces: A comparison between solution phase and colloidal system.
Abstract Present work reports on Cytochrome C (Cyt C) adsorption from solution and as sub-micron sized colloidal particles (pH 7.5) at fluid/solid interface. The colloidal particles from 2 methods ((method 1 freeze-thaw); method 2-Decane/water interface) have been characterized by UV-Visible spectroscopy, Static and Time resolved Fluorescence spectra and Circular dichroic spectroscopy. Morphology and size studies indicate that the colloids are solid and well-packed. Using Quartz crystal microbalance with dissipation, elastic compliance of the protein on quartz surface has been monitored. Properties of the protei...
Source: International Journal of Biological Macromolecules - July 9, 2019 Category: Biochemistry Authors: Jaganathan M, Easwaramoorthy S, Dhathathreyan A Tags: Int J Biol Macromol Source Type: research

Preparation and characterization of electrospun poly(lactic acid)-chitosan (core-shell) nanofibers with a new solvent system.
Abstract Poly(lactic acid)-chitosan (PLA-CS) core-shell nanofibers were successfully fabricated by coaxial electrospinning technique using a new solvent system for the core and shell solutions, dimethylformamide for the core solution and trifluoro-acetic acid/acetic acid (90/10 v/v) for the shell solution. The formation of the core-shell structure of the nanofibers with this new solvent system was confirmed by transmission electron microscopy (TEM) and attenuated total reflectance Fourier transform spectroscopy (ATR-FTIR). Morphology of the as-spun nanofibers was investigated by scanning electron microscopy (SEM) ...
Source: International Journal of Biological Macromolecules - July 9, 2019 Category: Biochemistry Authors: Afshar S, Rashedi S, Nazockdast H Tags: Int J Biol Macromol Source Type: research

In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan.
Abstract Fucoidan is a sulfated hetero-polysaccharide, found in cell-wall composition of brown algae. Recent studies have reported the role of fucoidan in the induction of Endoplasmic Reticulum (ER) stress-related cell death in cancer cells but the mechanism of action of fucoidan in cervical HeLa cells is not well-known. The purpose of this study was to investigate if fucoidan induces HeLa cells death through ER-stress-related cell death and G1 phase arrest. 200-600 μg/ml concentrations of fucoidan inhibited the proliferation of HeLa cells after 48 h of treatment while investigated normal cell lines (HaCaT:...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Niyonizigiye I, Ngabire D, Patil MP, Singh AA, Kim GD Tags: Int J Biol Macromol Source Type: research

Synthesis and characterization of carboxymethyl starch-g-polyacrylic acids and their properties as adsorbents for ammonia and phenol.
Abstract Cigarette smoke contains a lot of harmful chemicals which cause different diseases like cancer, heart disease, bronchitis and ulcer etc. Ammonia and phenol are among those chemicals which cause cancer, fibrosis, respiratory disorder and pneumonia. So, to remove ammonia and phenol from cigarette smoke, five different types of carboxymethyl starch-g-polyacrylic acids (CMS-g-PAAs) were synthesized by using different initiators, different mole ratio of acrylic acid to CMS anhydroglucose unit (AGU) and different amount of water. Three types of modified CMSs, CMS-g-PAA1, CMS-g-PAA3 and CMS-g-PAA4 were selected ...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Haroon M, Yu H, Wang L, Ullah RS, Haq F, Teng L Tags: Int J Biol Macromol Source Type: research

Dielectric relaxation studies of collagen - Surfactant complexes in aqueous buffer solution.
Abstract Dielectric relaxation studies of rat tail tendon collagen with anionic sodium dodecyl sulphate (SDS), cationic cetyltrimethyl ammonium bromide (CTAB) and nonionic Tween 80 in aqueous buffer solutions at four different temperatures have been carried out in the frequency range 1 GHz to 25 GHz using time domain dielectric spectroscopy. The frequency dependent normalized dielectric modulus function P of the collagen and surfactant solutions show up as peaks around 1-2 GHz which is due to the bound water around collagen macromolecule and surfactant micelles. For the collagen - surfactant complexes, the p...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Gopalakrishnan D, Balachandar V, Kumbharkhane AC, Sampathkumar R Tags: Int J Biol Macromol Source Type: research

Effects of different carbohydrate-binding modules on the enzymatic properties of pullulanase.
Abstract Some specific properties of pullulanases usually have a great deal to do with their specific N-terminal CBMs. This paper focusses on characters of the novel carbohydrate-binding module CBM68 from a thermostable pullulanase PulA. As to reveal its different properties with the common module CBM41/CBM41-X45 (from an acid pullulanase PulB), twelve recombinant pullulanases were constructed and named as Pul-1~Pul-12 respectively. Through comparative analysis of their thermostabilities, pH profiles, and kinetic parameters, some rules have been concluded. Compared with CBM41, CBM68 on the N-terminal of molecules ...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Zeng Y, Xu J, Fu X, Tan M, Liu F, Zheng H, Song H Tags: Int J Biol Macromol Source Type: research

Thermochemical research of chitosan complexes with sulfonated metallophthalocyanines.
Abstract The complexation processes of chitosan with cobalt(II)tetrasulfophthalocyanine (CoPc) and copper(II)tetrasulfophthalocyanine (CuPc) were studied calorimetrically in solution. It was established that CoPc forms two types of complexes with chitosan, while CuPc forms a single type of complex with chitosan, in which copper(II)tetrasulfophthalocyanine is in dimerized form. The complexes are thermodynamically stable, which was allowed to study them in a solid form by different methods. Joint application of DSC and TG/DTG methods allowed us to identify the temperature intervals for evaporation of physically and ...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Lebedeva NS, Guseinov SS, Yurina ES, Gubarev YA, Kiofman OI Tags: Int J Biol Macromol Source Type: research

The ameliorations of Ganoderma applanatum residue polysaccharides against CCl4 induced liver injury.
Abstract This work investigated the protective effects of Ganoderma applanatum residue polysaccharides (GRP) on the CCl4-induced hepatotoxicity. The results indicated that GRP showed significantly effects on preventing the increase of AST, ALT and ALP levels in serum, elevating the activities of SOD, GSH-Px and CAT, decreasing the contents of MDA and LPO, and reducing the CYP2E1 and TGF-β concentrations in CCl4-induced mice, respectively. Meanwhile, the levels of TNF-α and IL-6 were significantly decreased, while the value of IL-10 was increased by GRP treatment. Besides, the western blot assay showed t...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Gao Z, Yuan F, Li H, Feng Y, Zhang Y, Zhang C, Zhang J, Song Z, Jia L Tags: Int J Biol Macromol Source Type: research

Safe and efficient gene delivery based on rice bran polysaccharide.
Abstract Polysaccharides are capable of being modified by polycations to adjust their physical and chemical properties, which accordingly are considered as potential candidate materials for safe and efficient gene delivery. Here, we extracted and purified polysaccharides from rice bran, and their physicochemical properties were determined by various methods. Polyethyleneimine (PEI) modified rice bran polysaccharide (PRBP) was prepared by grafting RBP with low molecular weight PEI and the preparation were determined by FTIR. The potential of PRBP as a gene vector was systematically evaluated in vitro. The results s...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Wu S, Ni D, Yan Y, Pan X, Chen X, Guan J, Xiong X, Liu L Tags: Int J Biol Macromol Source Type: research

Conjugation of vitamin C with serum proteins: A potential application for vitamin delivery.
Abstract Vitamin C plays an important role in human health and therefore, the bioavailability and delivery of this micronutrient in solution have been the subject of intensive studies. Serum proteins are known to play an important role as drug delivery tools with important clinical applications. The conjugation of l-ascorbic acid with human serum albumin (HSA), bovine serum albumin (BSA) and beta-lactoglobulin (β-LG) was investigated in aqueous solution at physiological pH. Multiple spectroscopic methods, thermodynamic analysis and modeling were used to determine the binding efficacy of the vitamin C with ser...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Chanphai, Tajmir-Riahi HA Tags: Int J Biol Macromol Source Type: research

Laccase isoform diversity in basidiomycete Lentinus strigosus 1566: Potential for phenylpropanoid polymerization.
Abstract Three laccase isoforms with different physicochemical properties could be purified from culture liquid of basidiomycete Lentinus strigosus 1566 obtained during submerged cultivation. The purified laccases possessed individual selectivity in relation to different phenolic compounds. Laccases I, II, and III (60, 66, and 63 kDa respectively) were more active in acidic conditions at around 70 °C. However, in contrast to laccases I and II, laccase III retained its activity (8-30%) and stability during at least one week of incubation at neutral conditions that allows its biotechnological application car...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Kolomytseva MP, Myasoedova NM, Chernykh AM, Gaidina AS, Shebanova AD, Baskunov BP, Aschenbrenner J, Rosengarten JF, Renfeld ZV, Gasanov NB, Pinchuk IP, Classen T, Pietruszka J, Golovleva LA Tags: Int J Biol Macromol Source Type: research

Investigating the oxidative refolding mechanism of Cripto-1 CFC domain.
uvo M Abstract Using a combined approach based on MS, enzyme digestion and advanced MD studies we have determined the sequential order of formation of the three disulfide bridges of the Cripto-1 CFC domain. The domain has a rare pattern of bridges and is involved in the recognition of several receptors. The bridge formation order is C1-C4, C3-C5, C2-C6, however formation of C1-C4 plays no roles for the formation of the others. Folding is driven by formation of the C3-C5 bridge and is supported by residues lying within the segment delimited by these cysteines. We indeed observe that variants CFC-W123A and CFC-&Delt...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Iaccarino E, Sandomenico A, Corvino G, Focà G, Severino V, Russo R, Caporale A, Raimondo D, D'Abramo M, Alba J, Chambery A, Ruvo M Tags: Int J Biol Macromol Source Type: research

The application of BMP-12-overexpressing mesenchymal stem cells loaded 3D-printed PLGA scaffolds in rabbit rotator cuff repair.
This study investigates if the application of bone marrow-derived mesenchymal stem cells (BM-MSCs) loaded 3D-printed scaffolds could improve rotator cuff repair. The polylactic-co-glycolic acid (PLGA) scaffolds were fabricated by 3D print technology. Rabbit BM-MSCs were transfected with a recombinant adenovirus encoding bone morphogenic protein 12 (BMP-12). The effect of BM-MSCs loaded PLGA scaffolds on tendon-bone healing was assessed by biomechanical testing and histological analysis in a rabbit rotator cuff repair model. We found that the PLGA scaffolds had good biocompatible and biodegradable property. Overexpression o...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Chen P, Cui L, Chen G, You T, Li W, Zuo J, Wang C, Zhang W, Jiang C Tags: Int J Biol Macromol Source Type: research

Preparation of nanocellulose and lignin-carbohydrate complex composite biological carriers and culture of heart coronary artery endothelial cells.
Abstract Lignin-carbohydrate complexes, i.e. LCC-48 and LCC-72 were isolated with vibrational ball milling for 48 h and 72 h, respectively. Composite tubular carriers were prepared by casting film formation and crimping with the LCCs and cellulose nanofibrils (CNFs) as raw materials. The structures and chemical properties of different milling time LCCs were analyzed. The carriers were used to culture human coronary artery endothelial cells (HCAEC), and the activities of these cells were examined in vitro. The FT-IR and chemical composition results showed that the biocarriers were composed of lignin moieties an...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Zhao HK, Wei XY, Xie YM, Feng QH Tags: Int J Biol Macromol Source Type: research

Exploring the influence of brilliant blue G on amyloid fibril formation of lysozyme.
Abstract Evidence suggests that amyloid fibril mitigation/inhibition is considered a promising approach toward treating amyloid diseases. In this work, we first examined how amyloid fibrillogenesis of lysozyme was affected by BBG, a safe triphenylmethane compound with nice blood-brain-barrier-permeability, and found that shorter fibrillar species were formed in the lysozyme samples treated with BBG. Next, alterations in the features including the secondary as well as tertiary structure, extent of aggregation, and molecular distribution of lysozyme triggered by the addition of BBG were examined by various spectrosc...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: How SC, Hsin A, Chen GY, Hsu WT, Yang SM, Chou WL, Chou SH, Wang SS Tags: Int J Biol Macromol Source Type: research

Ductile and biodegradable poly (lactic acid) matrix film with layered structure.
Abstract Polylactide (PLA), as a biodegradable packing material, has attracted plenty of attention. However, some problems still limit the application of PLA in packing industry such as the inherent brittleness and low crack propagation resistance. In order to overcome these challenges, we blended PLA with a reactive toughening agent (Ethylene-Acrylic ester-Glycidyl methacrylate terpolymer) during extrusion and film processing. The glycidyl methacrylate groups in toughening agent offered some sites to react with COOH and OH groups of PLA thus leaded to a great interfacial compatibility. The proper compatibility wa...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Cao Z, Pan H, Chen Y, Han L, Bian J, Zhang H, Dong L, Yang Y Tags: Int J Biol Macromol Source Type: research

Measured collagen fibril response to arterial inflation using SAXS.
Abstract Arteries are elastic structures containing both elastin and collagen. While the high content of elastin is understood to be important for the elasticity of arteries with systolic and diastolic pressure pulses, the role of collagen in the elastic properties of arteries is less understood. Here we use small angle X-ray scattering to investigate the changes in arrangement of collagen fibrils and the strain experienced by collagen fibrils as arteries are inflated. Collagen fibrils re-orient to become more aligned in both the annular direction and radially as arteries inflate. With arterial pressures up to 32...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Wells HC, Sizeland KH, Kirby N, Hawley A, Mudie S, Cunningham CW, Haverkamp RG Tags: Int J Biol Macromol Source Type: research

Preparation and physical/chemical modification of galactomannan film for food packaging.
Abstract The physical (UV irradiation) and chemical (glutaraldehyde) modification of galactomannan (GMP) film was successfully prepared in this study. The data indicated that the UV irradiation with increasing dosage of photo-initiator could gradually consume the free hydroxyl groups in GMP to form the crosslinked networks, reducing the hydrophilic property and water vapor permeability of the modified film. The tensile strength of the obtained film with 20% sodium benzoate addition achieved 2.8 times higher than the control sample. In comparison, aldol reaction between hydroxyl groups in GMP and glutaraldehyde res...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Zhao N, Chai Y, Wang T, Wang K, Jiang J, Yang HY Tags: Int J Biol Macromol Source Type: research

Effect of glow discharge plasma on surface modification of chitosan film.
Abstract Glow discharge plasma (GDP) was used to modify chitosan films to obtain desirable properties. The chitosan films were treated with GDP of 0-800 W for 1 min, respectively. The molecular structure of chitosan films changed under GDP treatment. Chitosan films showed an enhancement of crystallinity with the increasing power of GDP, and the GDP treatment provided them with higher thermal stability, manifested as the exothermic peaks shifted from 291.5 °C (0 W) to 294.1 °C (800 W). Scanning electron microscope (SEM) indicated that the surface of chitosan films got rougher with 0-600 W GDP ...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Li Y, Wu C, Bai Y, Liu S, Yuan C, Ding T, Hu Y Tags: Int J Biol Macromol Source Type: research

Achieving cytochrome c fibril/aggregate control towards micro-platelets and micro-fibers by tuning pH and protein concentration: A combined morphological and spectroscopic analysis.
Abstract Cytochrome c is a metalloprotein with primary physiological functions in the respiratory chain and in the regulation of cell death signals. Investigating the mechanisms leading to cytochrome c fibril formation is of primary importance for understanding its misfunctioning and, in a wider perspective, for its technologic applications in the field of bio-nanoscience. In this work, we analyzed the morphology and the spectroscopic properties of cytochrome c aggregates, combining the outcomes from electron microscopy, fluorescence, infrared and Raman spectroscopies and making use of statistical tools for the da...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Nucara A, Carbone M, Ripanti F, Manganiello R, Postorino P, Carbonaro M Tags: Int J Biol Macromol Source Type: research

Chromatography and mass spectrometry-based approaches for perception of polysaccharides in wild and cultured fruit bodies of Auricularia auricular-judae.
Abstract Auricularia auricular-judae polysaccharides (AAPs) have been accepted as one important biological constituent. Quality analysis of A. auricular-judae is generally difficult due to technical issues in separating and detecting AAPs. Here, we describe a stepped chromatographic and mass spectrometric approach for discrimination and characterization of AAPs. HPSEC-MALLS-RID and GC-MS techniques have earlier been shown to be ineffective in evaluating wild and cultured AAPs based on molecular weight distributions and monosaccharide compositions. Nevertheless, direct ESI--MS oligosaccharide fingerprints coupled w...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Xia YG, Yu LS, Liang J, Yang BY, Kuang HX Tags: Int J Biol Macromol Source Type: research

Robust microfluidic construction of konjac glucomannan-based micro-films for active food packaging.
The objective of this study was to develop a novel active food packaging with high performance by using natural active compound. Here, a facile and green strategy was employed to construct the konjac glucomannan/polylactic acid/trans-cinnamic acid micro-films (KPTMF) via microfluidic spinning technology (MST). MST, a mild preparation approach, could remain the activities of natural compound during processing. Konjac glucomannan could drive the release of trans-cinnamic by using its hydrophilicity under our careful design. The results of fourier-transform infrared spectra and infrared images revealed that konjac glucomannan...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Lin W, Ni Y, Liu D, Yao Y, Pang J Tags: Int J Biol Macromol Source Type: research

Mussel-inspired antibacterial polydopamine/chitosan/temperature-responsive hydrogels for rapid hemostasis.
Abstract The aim of this study was to develop an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel. The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively. The gelling characteristic of HBC hydrogel was not influenced by composite CS and DOPA. The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate. In vitro antib...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Zhang X, Sun GH, Tian MP, Wang YN, Qu CC, Cheng XJ, Feng C, Chen XG Tags: Int J Biol Macromol Source Type: research

Development of three-dimensional bacterial cellulose/chitosan scaffolds: Analysis of cell-scaffold interaction for potential application in the diagnosis of ovarian cancer.
Abstract Bacterial cellulose (BC) has emerged as a biomaterial for diverse biomedical applications owing to its unique structural, physico-chemical, mechanical, and biological features. Its porous geometry and three-dimensional fibrous structure allow the impregnation of various materials into its matrix. The current study was aimed to fabricate 3D scaffolds of bacterial cellulose and chitosan (BC-Chi) through a one-step ex situ solution impregnation strategy and analyze the scaffold interaction with the ovarian cancer cell lines (A2780). Field emission scanning electron microscopy (FE-SEM) showed successful impre...
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Ul-Islam M, Subhan F, Islam SU, Khan S, Shah N, Manan S, Ullah MW, Yang G Tags: Int J Biol Macromol Source Type: research

Relationship between protein structure changes and in vitro digestion of preserved egg white during pickling.
This study would provide guidance for studying nutritional value and controlling quality of preserved eggs. PMID: 31295501 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 8, 2019 Category: Biochemistry Authors: Guo W, Zhao Y, Yao Y, Wu N, Xu M, Du H, Tu Y Tags: Int J Biol Macromol Source Type: research

Gamma radiation-induced crosslinked composite membranes based on polyvinyl alcohol/chitosan/AgNO3/vitamin E for biomedical applications.
Abstract Crosslinked hydrogel composite membranes based on polyvinyl alcohol (PVA) and chitosan-loaded AgNO3 and vitamin E were prepared using gamma irradiation. Chitosan has been used as antimicrobial blend materials to provide further biocompatibility for the prepared composite hydrogel membranes. The crosslinking reaction between PVA and chitosan owing to gamma irradiation was verified and characterized by FTIR analysis, while the morphology of hydrogel composite membranes was investigated by SEM. Important parameters affecting on hydrogel membranes formation, such as copolymer concentration, irradiation dose, ...
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Nasef SM, Khozemy EE, Kamoun EA, El-Gendi H Tags: Int J Biol Macromol Source Type: research

Synthesis of nanocomposites using xylan and graphite oxide for remediation of cationic dyes in aqueous solutions.
Abstract Due to the rapid development of industrialization, the water resources on which we depend are facing unprecedented challenges. Dyes, as an indispensable substance in our lives, have caused great pollution to the water resources in nature, and the removal of dyes from wastewater is becoming an important topic. A porous xylan/poly(acrylic acid)/graphite oxide nanocomposite was prepared by graft polymerization and used for adsorption of cationic ethyl violet dye in wastewaters in this paper. Various techniques, i.e., Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, elem...
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Fu C, Dong X, Wang S, Kong F Tags: Int J Biol Macromol Source Type: research

Enhancement of biocompatibility and carbohydrate absorption control potential of rosmarinic acid through crosslinking into microparticles.
Abstract Rosmarinic acid (RA), a bioflavonoid and antioxidant that exists in plants of the Lamiaceae family, was crosslinked into particles as poly(Rosmarinic Acid) (p(RA)) via an emulsion crosslinking method. The particles were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, solid state nuclear magnetic resonance 13C NMR spectroscopy, and thermal gravimetric analysis. The zeta potential values of p(RA) particles were determined at different pHs; the isoelectric point was estimated as pH 1.2. The release of monomeric RA from the particles at 37.5 °C was found to b...
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Sahiner M, Blake DA, Fullerton ML, Suner SS, Sunol AK, Sahiner N Tags: Int J Biol Macromol Source Type: research

Porcine Fc γRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction.
In this study, MARC-145 cel1 lines stably expressing mutated poFcγRIIb (MARC-poFcγRIIb-T and MARC-poFcγRIIb-CT) in cytoplasm were established and the capacity of poFcγRIIb mutants in mediating ADE of PRRSV was investigated. Our results showed that removal of cytoplasmic domain or disruption the tyrosine residue within ITIM (immunoreceptor tyrosine-based inhibition motif) of the poFcγRIIb abolished the ability of poFcγRIIb to mediate ADE of PRRSV. Furthermore, we found that SHIP1 and TBK1 were involved in poFcγRIIb-mediated ADE of PRRSV infection. Taken together, our findings indica...
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Wan B, Chen X, Li Y, Pang M, Chen H, Nie X, Pan Y, Qiao S, Bao D Tags: Int J Biol Macromol Source Type: research

High-pressure ultrasonic-assisted extraction of polysaccharides from Hovenia dulcis: Extraction, structure, antioxidant activity and hypoglycemic.
In this study, high-pressure ultrasonic-assisted extraction (HUE) technology was used for polysaccharide extraction from Hovenia dulcis (HDPS). A maximal extraction yield (11.81 ± 0.26%) was obtained using the response surface method (RSM) with optimized HUE conditions (Ultrasound power 330 W, 68 °C, and 22 min). Results showed that HDPS mainly contained neutral sugar (47.81 ± 1.56%), uronic acid (12.52 ± 0.15%), and protein (3.64 ± 0.24%), specifically. HDPS was comprised of mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose in a molar ratio of 3.20...
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Yang B, Wu Q, Luo Y, Yang Q, Wei X, Kan J Tags: Int J Biol Macromol Source Type: research

Selenium nanoparticles fabricated in laminarin polysaccharides solutions exert their cytotoxicities in HepG2 cells by inhibiting autophagy and promoting apoptosis.
In conclusion, these results indicated that LP-SeNPs exerted its cytotoxicity in HepG2 cells by inhibiting autophagy and inducing apoptosis. PMID: 31284007 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Cui D, Ma J, Liang T, Sun L, Meng L, Liang T, Li Q Tags: Int J Biol Macromol Source Type: research

Periodate oxidized hyaluronic acid-based hydrogel scaffolds for tissue engineering applications.
Abstract Modification of Hyaluronic acid (HA) with Sodium metaperiodate (NaIO4) results in the formation of oxidized HA (OHA). OHA containing multiple aldehyde groups can easily react with the materials having amino functionality via formation of Schiff base linkage, resulting in the formation of OHA based hydrogel scaffolds. These hydrogels have recently attracted considerable attention as scaffolds for tissue engineering (TE) applications. Thus, the aim of the present review is to give an overview on OHA based hydrogels as scaffolds and their potential application in the field of TE along with the method of synt...
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Pandit AH, Mazumdar N, Ahmad S Tags: Int J Biol Macromol Source Type: research

Polylactide cellulose-based nanocomposites.
This article reviews the studies conducted on these challenges of developing PLA cellulose-based nanocomposites including the difficulties of their processing and possible enhancements of their rheological, thermal, and mechanical properties. The investigations that have been conducted on PLA-CNC, PLA-CNF, and PLA-BC nanocomposites are separately discussed in this review article, while the studies on the development of PLA-nanocellulose blend nanocomposites and PLA-nanocellulose microcellular foams are also highlighted. PMID: 31284009 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Vatansever E, Arslan D, Nofar M Tags: Int J Biol Macromol Source Type: research

Fungal (1  → 3)-α-d-glucans as a new kind of biosorbent for heavy metals.
Fungal (1 → 3)-α-d-glucans as a new kind of biosorbent for heavy metals. Int J Biol Macromol. 2019 Jul 05;: Authors: Nowak K, Wiater A, Choma A, Wiącek D, Bieganowski A, Siwulski M, Waśko A Abstract Although the fungal ability to bind heavy metals has been known for many years, it was not determined which component is responsible for this process. The aim of the study was to isolate (1 → 3)-α-d-glucans from various fungi, select the most efficient compound in the biosorption of heavy metals, and determine its characteristics. The best α-glucan for treatment of aqu...
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Nowak K, Wiater A, Choma A, Wiącek D, Bieganowski A, Siwulski M, Waśko A Tags: Int J Biol Macromol Source Type: research

Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.
Abstract A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles. The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods. The easy separation, recovery, reusability, and high yields of the products make this protocol environmentally friendly and economically attractive. PMID: 31284011 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Mahdavinasab M, Hamzehloueian M, Sarrafi Y Tags: Int J Biol Macromol Source Type: research

Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.
Abstract The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions could be improved by modifying the particles with aminopropyltriethoxysilane (APTS) and then combining with chitosan (CS) into beads. After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction. The magnetic properties of APTS-Fe3O4/APT@CS were studied by a vibrating sample magnetometer, while their heat resistance and stability were characterized by thermal weight anal...
Source: International Journal of Biological Macromolecules - July 5, 2019 Category: Biochemistry Authors: Liang XX, Ouyang XK, Wang S, Yang LY, Huang F, Ji C, Chen X Tags: Int J Biol Macromol Source Type: research

Alginate-bioactive glass containing Zn and Mg composite scaffolds for bone tissue engineering.
Abstract Past researches on bone regeneration field have shown the positive impacts of the presence of Zinc and Magnesium ions in the bioactive glasses composition. However, there is no dedicated work on the effect of aforementioned bio-glass on the polymer matrix composites. The key idea of the approach is to improve antibacterial efficacy, biological activity and mechanical properties of the bone composite scaffolds by incorporating bioactive glasses containing Zinc and Magnesium into alginate networks. The prepared scaffolds were characterized by SEM, ATR-FTIR and XRD analysis. Compression strength of obtained ...
Source: International Journal of Biological Macromolecules - July 4, 2019 Category: Biochemistry Authors: Zamani D, Moztarzadeh F, Bizari D Tags: Int J Biol Macromol Source Type: research

Zein-casein-lysine multicomposite nanoparticles are effective in modulate the intestinal permeability of ferulic acid.
The objective of this study was to develop zein-casein-lysine nanoparticles to modulate the intestinal permeability of ferulic acid (FA), a bioactive compound with proven antioxidant properties. The nanoparticles were obtained by a liquid-liquid dispersion method and were characterized in terms of mean size, polydispersity index, zeta potential, association efficiency (AE), in vitro drug release, x-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The in vitro intestinal permeability of nanoparticles was evaluated through Caco-2 and Caco-2/HT29-MTX monoculture and co-culture models, respectively. N...
Source: International Journal of Biological Macromolecules - July 4, 2019 Category: Biochemistry Authors: Heep G, Almeida A, Marcano R, Vieira D, Mainardes RM, Khalil NM, Sarmento B Tags: Int J Biol Macromol Source Type: research

γVPE plays an important role in programmed cell death for xylem fiber cells by activating protease CEP1 maturation in Arabidopsis thaliana.
γVPE plays an important role in programmed cell death for xylem fiber cells by activating protease CEP1 maturation in Arabidopsis thaliana. Int J Biol Macromol. 2019 Jul 04;: Authors: Cheng Z, Zhang J, Yin B, Liu Y, Wang B, Li H, Lu H Abstract The vacuolar processing enzyme (VPE) plays an important role in PCD and was originally identified as the proteinase responsible for the maturation and activation of vacuolar proteins in plants. We found that γVPE is involved in PCD of xylem fiber cells through the activation of CEP1 proproteases into mature protease in Arabidopsis. The γVPE pro...
Source: International Journal of Biological Macromolecules - July 4, 2019 Category: Biochemistry Authors: Cheng Z, Zhang J, Yin B, Liu Y, Wang B, Li H, Lu H Tags: Int J Biol Macromol Source Type: research

Short-time microwave treatment affects the multi-scale structure and digestive properties of high-amylose maize starch.
Abstract Microwave processing is a suitable technology for starch-based food processing. This work investigated the changes of structures and properties of high-amylose maize starch (HAMS) during short-time microwave irradiation (1-4 min). After 1 min of treatment, short amylopectin chains (DP 6-36) and intermediate amylose chains (DP 150-2000) of HAMS were partially broken down. Compared with native HAMS, treated HAMS (1 min) had the higher relative crystallinity, the intensity of the 9 nm lamellar peak, and fluorescence intensity under CLSM. Moreover, 1-min microwaving caused the lower viscosity and high...
Source: International Journal of Biological Macromolecules - July 4, 2019 Category: Biochemistry Authors: Zhong Y, Liang W, Pu H, Blennow A, Liu X, Guo D Tags: Int J Biol Macromol Source Type: research

Development of multi-epitope driven subunit vaccine against Fasciola gigantica using immunoinformatics approach.
Abstract Fascioliasis, a serious helminth disease of the livestock population, results from infection with the parasite Fasciola. Despite the alarming increase in drug resistance, a safe and fully effective vaccine for fascioliasis is still not available. In the present study, we employed high-throughput immunoinformatics approaches to design a multi-epitope based subunit vaccine using seven important F. gigantica proteins (cathepsin B, cathepsin L, leucyl aminopeptidase, thioredoxin glutathione reductase, fatty acid binding protein-1, saposin-like protein-2, and 14-3-3 protein epsilon). The CTL, HTL, and B-cell e...
Source: International Journal of Biological Macromolecules - July 4, 2019 Category: Biochemistry Authors: Kalita P, Lyngdoh DL, Padhi AK, Shukla H, Tripathi T Tags: Int J Biol Macromol Source Type: research

Facile preparation of biomass lignin-based hydroxyethyl cellulose super-absorbent hydrogel for dye pollutant removal.
Abstract The severe preparation process, poor swelling properties and mechanical properties of traditional cellulose and polyvinyl alcohol (PVA) composite hydrogels heavily limited their practical applications. To solve these issues, we use long-chain hydroxyethyl celluloses (HECs) as framework backbones, short-chain PVAs as branched chains, lignin molecules as extended crosslinkers and epichlorohydrin (ECH) molecules as crosslinkers to prepare lignin-based hydroxyethyl cellulose-PVA (LCP) super-absorbent hydrogels in the alkaline aqueous solution under mild reaction conditions, demonstrating high swelling ratio o...
Source: International Journal of Biological Macromolecules - July 4, 2019 Category: Biochemistry Authors: Huang S, Wu L, Li T, Xu D, Lin X, Wu C Tags: Int J Biol Macromol Source Type: research

In-depth mapping of the seed phosphoproteome and N-glycoproteome of Tartary buckwheat (Fagopyrum tataricum) using off-line high pH RPLC fractionation and nLC-MS/MS.
Abstract The seed of Tartary buckwheat (Fagopyrum tataricum) is rich in nutrients and functional ingredients and is recommended as a healthy cereal food. The total proteins of Tartary buckwheat seed (TBS) were extracted and digested; then, the phosphopeptides and glycopeptides were separately enriched and identified by nano liquid chromatography/tandem mass spectrometry. A total of 2613 phosphorylation sites from 1670 phosphoproteins and 404 N-glycosylation sites from 285 N-glycoproteins were identified in TBS. Function and pathway analyses showed that TBS phosphoproteins were significantly enriched in transport, ...
Source: International Journal of Biological Macromolecules - July 4, 2019 Category: Biochemistry Authors: Geng F, Liu X, Wang J, He R, Zhao J, Xiang D, Zou L, Peng L, Zhao G Tags: Int J Biol Macromol Source Type: research

Formulation and optimization of a novel TiO2/calcium alginate floating photocatalyst.
Abstract For the first time, TiO2 immobilized in alginate polymer was modified to turn it into a buoyant photocatalyst for achieving both higher radiance utilization efficiency and easier post-treatment separation. In this work, the low-density TiO2 alginate-based photocatalyst was synthesized by ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH). The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology. It wa...
Source: International Journal of Biological Macromolecules - July 4, 2019 Category: Biochemistry Authors: Dalponte I, de Sousa BC, Mathias AL, Jorge RMM Tags: Int J Biol Macromol Source Type: research

Carboxypeptidase A4 promotes cell growth via activating STAT3 and ERK signaling pathways and predicts a poor prognosis in colorectal cancer.
In this study, we investigated the functions and mechanisms by which CPA4 promotes CRC progression. Quantitative real-time PCR (qRT-PCR) and western blot showed that CPA4 mRNA and CPA4 protein levels were up-regulated in CRC compared to levels in adjacent normal tissue. Immunohistochemistry (IHC) results indicating high CPA4 levels were positively associated with poor prognoses. In addition, Cell Counting Kit-8 (CCK-8), colony formation, flow cytometry, and transwell assays demonstrated that CPA4 overexpression facilitated the growth of CRC cells, whereas CPA4 knockdown resulted in decreased proliferation, G1/S phase trans...
Source: International Journal of Biological Macromolecules - July 4, 2019 Category: Biochemistry Authors: Pan H, Pan J, Ji L, Song S, Lv H, Yang Z, Guo Y Tags: Int J Biol Macromol Source Type: research

Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation.
Abstract Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale. Structural characterization revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, ...
Source: International Journal of Biological Macromolecules - July 4, 2019 Category: Biochemistry Authors: Zeng YJ, Yang HR, Wu XL, Peng F, Huang Z, Pu L, Zong MH, Yang JG, Lou WY Tags: Int J Biol Macromol Source Type: research