Integrated preparation of functional lignin nanoparticles and levulinic acid directly from the pre-hydrolysis liquor of poplar wood
In this study, we proposed a green and simple strategy to simultaneously prepare size-controlled functional lignin nanoparticles (LNPs) and levulinic acid (LA) from PHL as the raw material. The as-prepared LNPs exhibited remarkable stability thanks to the presence of saccharides with abundant oxygen-containing groups and surface charges, which prevented aggregation and maintained long-term storage stability. Trace amounts of the LNPs (≤ 0.2 wt%) could stabilize various Pickering emulsions, even with oil-to-water ratios as high as 5:5 (v/v). Subsequently, the remaining PHL was directly used to produce LA without adding a ...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Shunli Li Weikun Jiang Huimei Wang Jiliang Ma Jinghui Zhou Yu Liu Source Type: research

Antigenicity, epitope mapping, and intracellular distribution of the NSP7 α protein of porcine reproductive and respiratory syndrome virus
In this study, NSP7α was expressed in prokaryote (Escherichia coli) and purified. An NSP7α-ab-ELISA detection method was established, the NSP7α-ab-ELISA has 93.1 % coincidence rate with IDEXX PRRS X3 ab test kit. NSP7α antibody was detected in pig serum by ELISA 14 days following PRRSV infection. Three monoclonal antibodies (4H9, 3F2, and C10) against NSP7α prepared by a hybridoma technique were used for epitope mapping by indirect immunofluorescence. The 4H9, 3F2, and C10 antibodies all recognized the C-terminal 72-149 amino acid region of NSP7α. 4H9 reacted with amino acids 135-143, but 3F2 and C10 did not react wi...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Tao Wang Da-Song Xia Xiao-Xiao Tian Yong-Bo Yang Tong-Qing An Source Type: research

Physicochemical comparison of chitin characteristics in three major stored-product beetle pests: Implications for eco-friendly biofumigant toxicity
Int J Biol Macromol. 2024 Mar 15:130759. doi: 10.1016/j.ijbiomac.2024.130759. Online ahead of print.ABSTRACTThe present study investigates the chitin properties of stored-product insect pests and their association with the fumigant toxicity of garlic essential oil. Chitin isolates of Callosobruchus maculatus, Sitophilus oryzae, and Tribolium castaneum adults were characterized using FT-IR, XRD, EA, SEM-EDS, and NMR techniques. Fumigant toxicity assay was performed under airtight condition in glass vial. The S. oryzae contains highest chitin content (19 %), followed by T. castaneum (10 %) and C. maculatus (8 %). The degree ...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Pratiksha Prabhakar Gawali Veeresh Toragall Lokesh Madhurya Sudheer Kumar Yannam Subramanian Vendan Source Type: research

Self-supporting noncovalent Choline Alginate/Tannic Acid/Ag antibacterial films for strawberry preservation
Int J Biol Macromol. 2024 Mar 15:130936. doi: 10.1016/j.ijbiomac.2024.130936. Online ahead of print.ABSTRACTPackaging materials with peculiar antibacterial properties can shield off and inhibit microorganism proliferation, thus achieving packaging goals such as fresh-keeping, good hygiene, and biosafety. Especially, antibacterial films made of biocompatible substances have received wide attentions, which could effectively extend the shelf life, enhance food security, and guarantee economic benefits. Herein, a self-supporting hybrid antibacterial film was prepared based on non-covalently linked choline hydroxide (ChOH) and ...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Xue Zhang Xueqiao Li Huiling Zhang Shasha Jiang Mingze Sun Tao He Tao Zhang Wenna Wu Source Type: research

Irisin delays the onset of type 1 diabetes in NOD mice by enhancing intestinal barrier
This study investigates whether irisin could delay T1D onset by enhancing the colon intestinal barrier. Impaired colon intestinal barriers were observed in newly diagnosed T1D patients and non-obese diabetic (NOD) mice, worsening with age and accompanied by islet inflammation. Using an LPS-induced colonic inflammation model, a dose-dependent impact of LPS on colon cells irisin expression, secretion, and barrier function was revealed. Exogenous irisin demonstrated remarkable effects, mitigating islet insulitis, enhancing energy expenditure, and alleviating autoimmune symptoms by reducing colon intestinal permeability. Singl...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Yujing Sun Yilin Wang Ziang Lin Fuhua Zhang Yan Zhang Tongxin Ren LiNa Wang Qincheng Qiao Mengyang Shen Juncheng Wang Youchen Song Yu Sun Peng Lin Source Type: research

A novel complex coupling agent for enhancing the compatibility between collagen fiber and natural rubber: A utilization strategy for leather wastes
In this study, we developed a novel complex coupling agent (CCA) to enhance the compatibility between CF and NR. The CCA was synthesized through a coordination reaction between Cr(III) and α-methacrylic acid (MAA). Cr(III) in the coupling agent coordinates with the active groups in CFs, while the unsaturated double bonds in MAA facilitate covalent crosslinking between the CCA and NR, improving compatibility. The coordination bonding between CF and NR exhibits strong interfacial interaction, endowing the composites with desirable mechanical properties. Moreover, the proposed method is an economical and green approach that ...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Xiaoyu Li Chao Lei Junyu Wan Weixing Xu Jianfei Zhou Bi Shi Source Type: research

Fabrication of gelatin-polyester based biocomposite scaffold via one-step functionalization of melt electrowritten polymer blends in aqueous phase
The objective of the current study is to control the phase separation between saturated and unsaturated polyesters occurring in the melt electro-writing process and utilization of this phenomenon to improve efficiency of biofunctionalization at the interface of scaffold via Aza-Michael addition reaction. Electron-deficient triple bonds of PE spots on the fibers exhibit good potential for the biofunctionalization via the aza-Michael addition reaction. PE spots are found to be pronounced in which blend compositions are PCL-PE as 90:10 and 75:25 %. The biofunctionalization of scaffold is monitored through CN bond formation ap...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: B üşra Köksal R ümeysa Bilginer Kartal Ufuk Saim Gunay Hakan Durmaz Ahu Arslan Y ıldız Ümit Hakan Yıldız Source Type: research

Insights into the preparation of zein nanoparticles by continuous membrane nanoprecipitation
Int J Biol Macromol. 2024 Mar 15:130935. doi: 10.1016/j.ijbiomac.2024.130935. Online ahead of print.ABSTRACTNanoparticles (NPs) preparation is limited to an exclusive use in batch processes and small-scale formulations. The use of membranes as high-performance micromixers is expected to open new scenarios to overcome limitations of conventional nanoprecipitation system such as stirred tank (ST) nanoprecipitation. The ability of the porous membrane to add uniformly one phase to another and govern their mixing at the membrane interface seems to be an important parameter for obtaining uniform NPs. Inorganic membranes (pore si...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: A Condello E Piacentini L Giorno Source Type: research

Deoxycholic acid inhibits ASFV replication by inhibiting MAPK signaling pathway
This study provides a theoretical basis for research on ASF pathogenesis and for antiviral drug development.PMID:38493816 | DOI:10.1016/j.ijbiomac.2024.130939 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Qi Gao Yifan Xu Yongzhi Feng Xiaoyu Zheng Ting Gong Qiyuan Kuang Qinxin Xiang Lang Gong Guihong Zhang Source Type: research

Nutritive profile, pharmaceutical potentials, and structural analysis of multifunctional bioactive fungal polysaccharides-A review
Int J Biol Macromol. 2024 Mar 15:130893. doi: 10.1016/j.ijbiomac.2024.130893. Online ahead of print.ABSTRACTAsian nations have long used edible fungi as food and medicine. Polysaccharides are among the main building units of the cell walls of fungi. Fungal polysaccharides have been documented in the medicinal and industrial sectors as products with a vast array of various biological activities and applications such as antitumor, antioxidant, anticancer, immunomodulation, and antiviral activities, etc. The goal of this review is to give insights into the various biological activities of mushroom polysaccharides and their po...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Marwa O Elnahas Waill A Elkhateeb Ghoson M Daba Source Type: research

Selective activation of IFN γ-ipilimumab enhances the therapeutic effect and safety of ipilimumab
CONCLUSION: IFNγ-ipilimumab improved the effectiveness of ipilimumab while reducing its side effects. It is likely that future immunotherapies would rely on such antibodies to activate local cancer cells or immune cells, thereby increasing the therapeutic effectiveness of cancer treatments and ensuring their safety.PMID:38493818 | DOI:10.1016/j.ijbiomac.2024.130945 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Yi-Jung Huang Kai-Wen Ho Tian-Lu Cheng Yen-Tseng Wang Shi-Wei Chao Bo-Cheng Huang Yu-Shu Chao Chia-Yu Lin Yun-Han Hsu Fang-Ming Chen Chih-Hung Chuang Source Type: research

Synergistic impact of natural gums and crosslinkers on the properties of oilseed meals based biopolymeric films
In this study, de-oiled cakes are used to create biopolymeric films. Three widely accessible oilseed meals viz. flaxseed, soybean, and mustard were gathered, ground, and sieved. A film forming suspension of defatted meals along with natural gums (acacia and xanthan gum) and crosslinkers (citric acid and glutaraldehyde) were formed. The suspension was cast into petri dishes and dried to produce smooth and even films. The physical, functional, color, thermal and morphological properties of the oilseed meals-gums crosslinked biopolymeric film were evaluated and statistical analysis was performed. The solubility was found to b...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Ruchi Rani Laxmikant S Badwaik Source Type: research

Carrageenan-xanthan nanocomposite film with improved bioadhesion and permeation profile in human skin: A cutaneous-friendly platform for ketoprofen local delivery
Int J Biol Macromol. 2024 Mar 15:130864. doi: 10.1016/j.ijbiomac.2024.130864. Online ahead of print.ABSTRACTKetoprofen (KET), commonly used for inflammation in clinical settings, leads to systemic adverse effects with prolonged use, mitigated by topical administration. Nanotechnology-based cutaneous forms, like films, may enhance KET efficacy. Therefore, this study aimed to prepare and characterize films containing KET nanoemulsions (F-NK) regarding mechanical properties, chemical composition and interactions, occlusive potential, bioadhesion, drug permeation in human skin, and safety. The films were prepared using a κ-ca...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Marcel Henrique Marcondes Sari Camila Parcianello Saccol Vanessa Neuenschwander Cust ódio Lucas Saldanha da Rosa Juli ê Silveira da Costa Andr é Ricardo Fajardo Luana Mota Ferreira Let ícia Cruz Source Type: research

Extraction, purification, structural characteristics, bioactivity and potential applications of polysaccharides from Avena sativa L.: A review
Int J Biol Macromol. 2024 Mar 15:130891. doi: 10.1016/j.ijbiomac.2024.130891. Online ahead of print.ABSTRACTAvena sativa L. (A. sativa L.), commonly known as oat, is a significant cereal grain crop with excellent edible and medicinal value. Oat polysaccharides (OPs), the major bioactive components of A. sativa L., have received considerable attention due to their beneficial bioactivities. However, the isolation and purification methods of OPs lack innovation, and the structure-activity relationship remains unexplored. This review emphatically summarized recent progress in the extraction and purification methods, structural...
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Dan Li Mengjie Chen Xianwei Meng Yuan Sun Rui Liu Tiedong Sun Source Type: research

Starch-based biodegradable composites: Effects of in-situ re-extrusion on structure and performance
This study presents a novel method for fabricating starch-degradable composite films by in-situ re-extrusion, providing new insights into the development of starch packaging materials.PMID:38493822 | DOI:10.1016/j.ijbiomac.2024.130869 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - March 17, 2024 Category: Biochemistry Authors: Liping Wang Dexiang Li Lei Ye Chaohui Zhi Tao Zhang Ming Miao Source Type: research