Polylactic acid/Gemini surfactant modified clay bio-nanocomposites: Morphological, thermal, mechanical and barrier properties
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00427-X. doi: 10.1016/j.ijbiomac.2021.02.135. Online ahead of print.ABSTRACTTo improve PLA's properties and overcome its drawbacks such us poor thermal stability, resistance and gas barrier properties, several studies have been performed using different nanofillers. In this work, PLA nanocomposites reinforced by three organoclays, OMt(8-4-8), OMt(10-4-10) and OMt(12-4-12) at various weight percentages (1 and 3 wt%) were prepared by melt mixing using a twin-screw extruder. The organoclays were obtained from sodium montmorillionite and gemini surfactants bearing different hydrop...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Khadidja Taleb Isabelle Pillin Yves Grohens Salima Saidi-Besbes Source Type: research

Performance and multi-scale investigation on the phase miscibility of poly(lactic acid)/amided silica nanocomposites
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00396-2. doi: 10.1016/j.ijbiomac.2021.02.117. Online ahead of print.ABSTRACTIn this work, amino-functionalized nano-SiO2 (m@g-SiO2) was synthesized through coupling reaction on the surface of nano-SiO2. Moreover, the optimum preparation conditions of m@g-SiO2 were selected via orthogonal experiments as follows: reaction temperature of 80 °C, reaction time of 8 h, the mass ratio of stearic acid, N,N'‑carbonyldiimidazole, imidazole hydrochloride and g-SiO2 of 0.5:0.7:0.7:1. Fourier transform infrared spectroscopy, static angle measurement and X-ray photoelectron spectrosco...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Dawei Luo Weijun Zhen Chengyuan Dong Ling Zhao Source Type: research

Design of PAMAM grafted chitosan dendrimers biosorbent for removal of anionic dyes: Adsorption isotherms, kinetics and thermodynamics studies
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00397-4. doi: 10.1016/j.ijbiomac.2021.02.118. Online ahead of print.ABSTRACTPAMAM grafted chitosan as biocompatible adsorbent was synthesized through Michael addition of methyl acrylate followed by amidation of ethylenediamine on the chitosan backbone. Then, the adsorption capacity of bioadsorbents were assessed by employing two anionic dyes. The adsorption experiments were carried out using a batch adsorption system. The influence of various operational variables such as different PAMAM generations, pH, adsorbent dosage, contact time, initial dye concentration and temperature...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Fatemeh Banisheykholeslami Morteza Hosseini Ghasem Najafpour Darzi Source Type: research

Alginate/gelatin encapsulation promotes NK cells differentiation potential of bone marrow resident C-kit < sup > + < /sup > hematopoietic stem cells
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00416-5. doi: 10.1016/j.ijbiomac.2021.02.131. Online ahead of print.ABSTRACTThe ability of natural killer (NK) cells to destroy cancerous cells with no prior sensitization has made them attractive candidates for cell therapy. The application of hydrogels must be notified as cell delivery vehicles in cell differentiation. The present study was conducted to investigate the effect of alginate-gelatin encapsulation on NK cell differentiation potential of C-kit+ cells. C-kit+ cells were differentiated to NK cells under both encapsulated and un-encapsulated conditions. Next, the cel...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Ezzatollah Fathi Raheleh Farahzadi Behnaz Valipour Source Type: research

Scar free healing of full thickness diabetic wounds: A unique combination of silver nanoparticles as antimicrobial agent, calcium alginate nanoparticles as hemostatic agent, fresh blood as nutrient/growth factor supplier and chitosan as base matrix
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00424-4. doi: 10.1016/j.ijbiomac.2021.02.133. Online ahead of print.ABSTRACTHealing of diabetic wounds present a big challenge due to insufficient vascular supply and bacterial infection. We developed chitosan based biodegradable polymeric hydrogel containing silver nanoparticles (AgNPs) as antimicrobial agent and calcium alginate nanoparticles (Ca-AlgNps) as hemostatic agent to address this problem. The prepared Chitosan/Ca-AlgNps/AgNPs hydrogel showed broad spectrum antimicrobial properties against both Gram negative (E. coli, P. aeruginosa) and Gram positive (B. subtilis, S...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Meenakshi Choudhary Priyanka Chhabra Amit Tyagi Harpal Singh Source Type: research

Physicochemical characterization of Amaranth starch insulated by mechanical separations
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00429-3. doi: 10.1016/j.ijbiomac.2021.02.138. Online ahead of print.ABSTRACTThis work focused on the physicochemical properties of isolated Amaranth starch. Inductively coupled plasma (ICP) showed that amaranth is low-lipid calcium and magnesium source for the human diet. Scanning Electron Microscopy showed that isolated granules are in the range of sub and micro size. DSC starch thermogram showed a gelatinization temperature of 67.9 °C and an enthalpy of 10. 6 J/g suggesting the presence of an ordered crystalline structures. High-resolution X-ray diffraction showed the is...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Elsa Guti érrez-Cortez Ezequiel Hernadez-Becerra Sandra M Londo ño-Restrepo Mario E Rodr íguez-García Source Type: research

Cobalt doped nano-hydroxyapatite incorporated gum tragacanth-alginate beads as angiogenic-osteogenic cell encapsulation system for mesenchymal stem cell based bone tissue engineering
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00426-8. doi: 10.1016/j.ijbiomac.2021.02.136. Online ahead of print.ABSTRACTAngiogenic-osteogenic cell encapsulation system is a technical need for human mesenchymal stem cell (hMSC)-based bone tissue engineering (BTE). Here, we have developed a highly efficient hMSC encapsulation system by incorporating bivalent cobalt doped nano-hydroxyapatite (HAN) and gum tragacanth (GT) as angiogenic-osteogenic components into the calcium alginate (CA) beads. Physico-chemical characterizations revealed that the swelling and degradation of HAN incorporated CA-GT beads (GT-HAN) were 1.34 fo...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Senthilguru Kulanthaivel Tarun Agarwal V S Sharan Rathnam Kunal Pal Indranil Banerjee Source Type: research

Cationic polymer-modified Alhagi honey polysaccharide PLGA nanoparticles as an adjuvant to induce strong and long-lasting immune responses
In this study, three kinds of cationic polymer were used to coat AHP-encapsulated PLGA nanoparticles (AHPP) to build positively charged antigen carriers. Among them, H5N1-loaded PEI-AHPP formulation could induce highest hemagglutination inhibition (HI) titer, IgG-subtype, and cytokines, activated dendritic cells (DCs) in lymph nodes, and CD3e+CD4+ and CD3e+CD8a+ T cells in the spleen of immunized mice. PEI-AHPP could stimulate DCs to highly express MHCI and MHCII molecules and had good antigen slow-release effect at the injected site along with lymph node targeting. These findings demonstrate that PEI-AHPP has the potentia...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Adelijiang Wusiman Wenming Jiang Lin Yu Tianyu Zhu Jin He Zhenguang Liu Ruonan Bo Jiaguo Liu Deyun Wang Source Type: research

A pH indicator film based on chitosan and butterfly pudding extract for monitoring fish freshness
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00428-1. doi: 10.1016/j.ijbiomac.2021.02.137. Online ahead of print.ABSTRACTA pH indicator film was prepared by mixing natural polymeric chitosan (CH) with natural dye from butterfly pudding extract (BP). The films were determined by color changes at different pH value, absorbance, thickness, moisture content, swelling property, water contact angle, mechanical property, barrier property, and microstructure of films. The structural change of film was analyzed by Fourier transform infrared spectra. The application to monitor fish freshness was also studied. The prepared film was...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Jiatong Yan Rui Cui Yuyue Qin Lirong Li Minglong Yuan Source Type: research

Structure characterization of a pyruvated exopolysaccharide from Lactobacillus plantarum AR307
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00398-6. doi: 10.1016/j.ijbiomac.2021.02.119. Online ahead of print.ABSTRACTA pyruvated exopolysaccharide designated as LPE-1 was isolated and purified from the fermentation broth of Lactobacillus plantarum AR307 and characterized for its chemical structure. The results indicated that LPE-1 contained galactopyranose (Galp) and glucopyranose (Glcp) at a molar ratio of 2: 1. The weight-averaged molecular weight (Mw) of LPE-1 was 605 kDa, with a polydispersity index (PDI) of 1.57, intrinsic viscosity ([ƞ]) of 3.28 dL/g, Mark-Houwink-Sakurada exponent α of 0.65 and gyration...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Xiaowan Feng Hui Zhang Phoency F H Lai Zhiqiang Xiong Lianzhong Ai Source Type: research

Synthesis and characterization of ordered mesoporous silicas for the immobilization of formate dehydrogenase (FDH)
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00392-5. doi: 10.1016/j.ijbiomac.2021.02.114. Online ahead of print.ABSTRACTThis work studied the influence of the pore size and morphology of the mesoporous silica as support for formate dehydrogenase (FDH), the first enzyme of a multi-enzymatic cascade system to produce methanol, which catalyzes the reduction of carbon dioxide to formic acid. Specifically, a set of mesoporous silicas was modified with glyoxyl groups to immobilize covalently the FDH obtained from Candida boidinii. Three types of mesoporous silicas with different textural properties were synthesized and used a...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Giuseppe Pietricola Tonia Tommasi Melodj Dosa Enrico Camelin Emanuele Berruto Carminna Ottone Debora Fino Valentina Cauda Marco Piumetti Source Type: research

Preparation and characterization of antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00430-X. doi: 10.1016/j.ijbiomac.2021.02.139. Online ahead of print.ABSTRACTDue to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated. Chitosan-based composite films with and without cerium oxide nanoparticles (NPs) were prepared by a casting method. Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation. Characterizations of films...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Ezgi K ızılkonca Emrah Torlak F Bedia Erim Source Type: research

A colorimetric and fluorometric based dual readout approach for effective heparin sensing
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00425-6. doi: 10.1016/j.ijbiomac.2021.02.134. Online ahead of print.ABSTRACTDevising fluorescence-based turn-on probe for the specific and sensitive detection of Heparin is of utmost clinical importance. In this contribution, we have identified a molecular rotor based asymmetric cyanine probe, thiazole orange (TO), which enables an efficient colorimetric and fluorimetric detection of Heparin. TO undergoes the formation of emissive H-aggregates upon interaction with Heparin that displays an impressive emission enhancement of ~22 fold together with drastic changes in the absorpt...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Shrishti P Pandey Pamela Jha Prabhat K Singh Source Type: research

Injectable chitosan-quince seed gum hydrogels encapsulated with curcumin loaded-halloysite nanotubes designed for tissue engineering application
Int J Biol Macromol. 2021 Feb 20:S0141-8130(21)00393-7. doi: 10.1016/j.ijbiomac.2021.02.113. Online ahead of print.ABSTRACTThe goal of tissue engineering is to assemble functional constructs that restore, maintain, or improve damaged tissues or organs. Hydrogels formed with natural polymers display high potential in artificial scaffolds for tissue repair as they can resemble the extracellular matrices. Thus, the aim of this study was to design nanocomposite hydrogels of chitosan/oxidized-modified quince seed gum/curcumin-loaded in halloysite nanotubes (CS/OX-QSG/CUR-HNTs) for tissue engineering applications. The produced h...
Source: International Journal of Biological Macromolecules - February 23, 2021 Category: Biochemistry Authors: Leila Yavari Maroufi Marjan Ghorbani Source Type: research

Ions-induced gelation of alginate: Mechanisms and applications
This study is proposed to summarize the gelation mechanisms of alginate induced by different cations, mainly including H+, Ca2+, Ba2+, Cu2+, Sr2+, Zn2+, Fe2+, Mn2+, Al3+, and Fe3+. The mechanism of H+-induced gelation of alginate mainly depends on the protonation of carboxyl groups. Divalent ions-induced gelation of alginate show different selection towards G, M, and GM blocks. Trivalent ions can bind to carboxyl groups of uronates with no selection. The properties and applications of these ionotropic alginate gels are also discussed. The knowledge gained in this study would provide useful information for the practical app...
Source: International Journal of Biological Macromolecules - February 22, 2021 Category: Biochemistry Authors: Chuhuan Hu Wei Lu Analucia Mata Katsuyoshi Nishinari Yapeng Fang Source Type: research

Activated-carbon-filled agarose hydrogel as a natural medium for seed germination and seedling growth
Int J Biol Macromol. 2021 Feb 19:S0141-8130(21)00376-7. doi: 10.1016/j.ijbiomac.2021.02.097. Online ahead of print.ABSTRACTAn activated-carbon-filled agarose (Agar-AC) hydrogel containing various concentrations of activated carbon (AC) was successfully fabricated through a simple solvent cast technique. Compared to pure agarose hydrogels, Agar-AC hydrogels exhibited excellent mechanical properties, good thermal stability and a highly developed pore structure. The Agar-AC hydrogels also showed a certain degree of improvement in water retention performance, while their swelling ratio decreased with the addition of AC. The in...
Source: International Journal of Biological Macromolecules - February 22, 2021 Category: Biochemistry Authors: Lu Cao Na Li Source Type: research

Preparation of polylactic acid/TiO < sub > 2 < /sub > /GO nano-fibrous films and their preservation effect on green peppers
Int J Biol Macromol. 2021 Feb 18:S0141-8130(21)00405-0. doi: 10.1016/j.ijbiomac.2021.02.125. Online ahead of print.ABSTRACTPolylactic acid (PLA)/nano-TiO2(TiO2 NPs)/Graphene oxide (GO) nano-fibrous films were prepared by ultrasonic assisted electrostatic spinning technology, and the effects of TiO2 NPs:GO mass ratio and ultrasonic power on film morphology and mechanical, thermal, barrier and antibacterial properties were investigated. The addition of TiO2 NPs and GO can significantly increase the tensile strength and elongation at the break of PLA nano-fibrous films, and improve the water barrier properties of the nano-fib...
Source: International Journal of Biological Macromolecules - February 21, 2021 Category: Biochemistry Authors: Xiaorong Dong Xue Liang Yuting Zhou Kaiwen Bao Dur E Sameen Saeed Ahmed Jianwu Dai Wen Qin Yaowen Liu Source Type: research

Adsorption behavior of copper ions using crown ether-modified konjac glucomannan
Int J Biol Macromol. 2021 Feb 18:S0141-8130(21)00408-6. doi: 10.1016/j.ijbiomac.2021.02.129. Online ahead of print.ABSTRACTA novel supramolecular polysaccharide composite [KGM + DB18C6] was prepared from konjac glucomannan (KGM) and dibenzo-18-crown-6 (DB18C6) using ceric ammonium nitrate as initiator. The products were characterized by FTIR, TG, DSC, UV-Vis, XRD, solid-state 13C NMR, and SEM. Due to the introduction of crown ether, [KGM + DB18C6] showed good adsorption performance for Cu2+ in aqueous, and the maximum adsorption capacity was 194 mg/g under the optimal adsorption condition. The adsorption kinetics of [KGM +...
Source: International Journal of Biological Macromolecules - February 21, 2021 Category: Biochemistry Authors: Lianxiong Guan Huiting Kang Wei Liu Dating Tian Source Type: research

Metabolic engineering of Methylorubrum extorquens AM1 for poly (3-hydroxybutyrate-co-3-hydroxyvalerate) production using formate
This study shows that a PHBV copolymer with a high proportion of 3HV can be synthesized using formate, a C1 carbon source, through metabolic engineering and supplementation with short-chain fatty acids.PMID:33610606 | DOI:10.1016/j.ijbiomac.2021.02.092 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - February 21, 2021 Category: Biochemistry Authors: Jihee Yoon Woojin Chang Seung-Hwan Oh Soo-Hyung Choi Yung-Hun Yang Min-Kyu Oh Source Type: research

Chitosan/polycaprolactone multilayer hydrogel: A sustained Kartogenin delivery model for cartilage regeneration
Int J Biol Macromol. 2021 Feb 18:S0141-8130(21)00401-3. doi: 10.1016/j.ijbiomac.2021.02.122. Online ahead of print.ABSTRACTCartilage regeneration using biomaterial-guided delivery systems presents improved therapeutic efficacy of the biomolecules while minimizing side effects. Here, our hypothesis was to design a multilayer scaffold of chitosan (CS) hydrogel and polycaprolactone (PCL) mat to enhance the mechanical properties, integrity and stability of CS, especially for subsequent in vivo transplantation. After conjugation of the Kartogenin (KGN) into this structure, its gradual release can promote chondrogenesis of mesen...
Source: International Journal of Biological Macromolecules - February 21, 2021 Category: Biochemistry Authors: Arezou Baharlou Houreh Elahe Masaeli Mohammad Hossein Nasr-Esfahani Source Type: research

Facile synthesis of Zr(IV)-crosslinked carboxymethyl cellulose/carboxymethyl chitosan hydrogel using PEG as pore-forming agent for enhanced phosphate removal
Int J Biol Macromol. 2021 Feb 17;176:558-566. doi: 10.1016/j.ijbiomac.2021.02.106. Online ahead of print.ABSTRACTZr(IV)-crosslinked carboxymethyl cellulose/carboxymethyl chitosan hydrogels were prepared using polyethylene glycol (PEG) as pore-forming agent. The hydrogels exhibited porous structure and the pore size increased with the PEG content. The obtained hydrogels were used as adsorbents for removal of phosphate from aqueous solutions. The hydrogel prepared with most amount of PEG displayed largest adsorption amount of phosphate. This result might be attributed to improvement of surface area and pore volume caused by ...
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: Jingjing Wang Jun Wei Source Type: research

Biophysical interaction between self-assembled branched DNA nanostructures with bovine serum albumin and bovine liver catalase
Int J Biol Macromol. 2021 Feb 17:S0141-8130(21)00374-3. doi: 10.1016/j.ijbiomac.2021.02.095. Online ahead of print.ABSTRACTBranched DNA (bDNA) nanostructures have emerged as self-assembled biomaterials and are being considered for biomedical applications. Herein, we report the biophysical interaction between self-assembled bDNA nanostructure with circulating protein bovine serum albumin (BSA) and cellular enzyme bovine liver catalase (BLC). The binding between bDNA and BSA or BLC was confirmed through the decrease in fluorescence spectra. The Stern-Volmer data supports for non-covalent bonding with ~1 binding site in case ...
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: Bineeth Baral Juhi Dutta Umakanta Subudhi Source Type: research

Synthesis of a green bigel using cottonseed oil/cannabis oil/alginate/ferula gum for quercetin release: Synergistic effects for treating infertility in rats
Int J Biol Macromol. 2021 Feb 17:S0141-8130(21)00400-1. doi: 10.1016/j.ijbiomac.2021.02.121. Online ahead of print.ABSTRACTAlthough therapeutic effect of quercetin (Quer) was reported on non-alcoholic fatty liver disease (NAFLD), destructive effects have been shown on male fertility due to its pro-oxidative properties. On the other hand, NAFLD impairs germ cells to produce sperm and leads to male infertility. Herein, a biocompatible and green bigel was designed for Quer delivery to prevent infertility induced by NAFLD as the increasing complications. Bigels were prepared using cottonseed oil/cannabis oil/alginate/ferula gu...
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: Seyedeh Neda Mousavi Elham Hosseini Mir Saeed Seyed Dorraji Shima Sheikh Mohammadi Zeinab Pourmansoori Mohammad Hossein Rasulifard Masumeh Doosti Hossein Chiti Source Type: research

A review of extracellular polysaccharides from extreme niches: An emerging natural source for the biotechnology. From the adverse to diverse!
Int J Biol Macromol. 2021 Feb 17:S0141-8130(21)00380-9. doi: 10.1016/j.ijbiomac.2021.02.101. Online ahead of print.ABSTRACTEvery year, new organisms that survive and colonize adverse environments are discovered and isolated. Those organisms, called extremophiles, are distributed throughout the world, both in aquatic and terrestrial environments, such as sulfurous marsh waters, hydrothermal springs, deep waters, volcanos, terrestrial hot springs, marine saltern, salt lakes, among others. According to the ecosystem inhabiting, extremophiles are categorized as thermophiles, psychrophiles, halophiles, acidophiles, alkalophilic...
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: Mayra Alejandra L ópez-Ortega Norberto Chavarr ía-Hernández Ma Del Roc ío López-Cuellar Adriana In és Rodríguez-Hernández Source Type: research

HPTLC-densitometry quantification of fructooligosaccharides from inulin hydrolysate
The objective of present research was to develop an easy, precise and accurate HPTLC densitometry method for quantification of fructooligosaccharides (FOSs) from inulin hydrolysate. The chromatographic separation of FOSs was performed on pre-coated silica gel (60, F254) TLC plates using a mobile phase (butanol: ethanol:water, 60:24:16), and densitometry evaluation of FOSs was performed at A500. Both kestose and nystose were successfully resolved with Rf value of 0.43 and 0.34, respectively. The accuracy, reliability and reproducibility of developed method was assessed by percent relative standard deviation of kestose and n...
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: R S Singh Taranjeet Singh Dhandeep Singh John F Kennedy Source Type: research

Development of microcapsules using chitosan and alginate via W/O emulsion for the protection of hydrophilic compounds by comparing with hydrogel beads
Int J Biol Macromol. 2021 Feb 17:S0141-8130(21)00368-8. doi: 10.1016/j.ijbiomac.2021.02.089. Online ahead of print.ABSTRACTIt is a critical challenge to protect hydrophilic compounds in food or pharmaceutical applications due to their strong tendency to leak out of the capsules into the external aqueous phase. In this work, we developed an encapsulation system that can protect hydrophilic ingredients using polyelectrolyte complexes prepared with chitosan and alginate via water-in-oil (W/O) emulsion. Unlike the traditional preparation of hydrogel beads, in which one material was added dropwise to another that had an opposit...
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: Yuxiao Wang Chen Tan Seyed Mohammad Davachi Peilong Li Philip Davidowsky Bing Yan Source Type: research

Ferulic acid loaded microspheres reinforced in 3D hybrid scaffold for antimicrobial wound dressing
Int J Biol Macromol. 2021 Feb 17:S0141-8130(21)00403-7. doi: 10.1016/j.ijbiomac.2021.02.124. Online ahead of print.ABSTRACTHere we report the preparation of biomimetic fibrin/chitosan/keratin hybrid scaffolds with a synergistic combination of ferulic acid loaded silica microspheres for antimicrobial wound dressing applications. The infrared and X-ray powder diffraction studies confirm the homogenous nature of the prepared hybrid scaffolds without any major interactions between the constituents. The developed hybrid scaffolds show good thermal, porosity, compression and water uptake properties. Scanning electron microscopic...
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: Singaravelu Sivakumar Ragothaman Murali Deepika Arathanaikotti Arun Gopinath Chinnaiyan Senthilkumar Satheshkumar Kesavan Balaraman Madhan Source Type: research

A platform for functionalization of cellulose, chitin/chitosan, alginate with polydopamine: A review on fundamentals and technical applications
Int J Biol Macromol. 2021 Feb 17:S0141-8130(21)00370-6. doi: 10.1016/j.ijbiomac.2021.02.091. Online ahead of print.ABSTRACTNature provides concepts and materials with interesting functionalities to be implemented in innovative and sustainable materials. In this review, it is illustrated how the combination of biological macromolecules, i.e. polydopamine and polysaccharides (cellulose, chitin/chitosan, alginate), enables to create functional materials with controlled properties. The mussel-adhesive properties rely on the secretion of proteins having 3,4-dihydroxyphenylalanine amino acid with catechol groups. Fundamental und...
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: Pieter Samyn Source Type: research

The mutation of SPI51, a protease inhibitor of silkworm, resulted in the change of antifungal activity during domestication
This study provided new insights into the antifungal mechanisms elicited by protease inhibitors in the cocoons of silkworms.PMID:33609582 | DOI:10.1016/j.ijbiomac.2021.02.076 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: Xiaolu Zhang Yuhui Ni Kaiyu Guo Zhaoming Dong Yuqing Chen Hongtao Zhu Qingyou Xia Ping Zhao Source Type: research

β-Glucans as a panacea for a healthy heart? Their roles in preventing and treating cardiovascular diseases
Int J Biol Macromol. 2021 Feb 17:S0141-8130(21)00366-4. doi: 10.1016/j.ijbiomac.2021.02.087. Online ahead of print.ABSTRACTCardiovascular diseases (CVDs) are the leading cause of death worldwide. Factors increasing the risks for CVD development are related to obesity, diabetes, high blood cholesterol, high blood pressure and lifestyle. CVD risk factors may be treated with appropriate drugs, but prolonged can use cause undesirable side-effects. Among the natural products used in complementary and alternative medicines, are the β-ᴅ-glucans; biopolymers found in foods (cereals, mushrooms), and can easily be produced by...
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: J éssica Wouk Robert F H Dekker Eveline A I F Queiroz Aneli M Barbosa-Dekker Source Type: research

Correlation between the synthesis of pullulan and melanin in Aureobasidium pullulans
Int J Biol Macromol. 2021 Feb 17:S0141-8130(21)00386-X. doi: 10.1016/j.ijbiomac.2021.02.108. Online ahead of print.ABSTRACTThe content of pullulan and melanin in 500 mutants of Aureobasidum pullulans obtained by ultraviolet mutagenesis were examined and statistically analyzed, and a strong positive correlation was found between them. The result was further confirmed by culturing wild type strain As3.3984 in different media. Then we constructed melanin-deletion mutant As-Δalb1 and pullulan-deletion mutant As-Δpul. As-Δalb1 was a melanin-free strain with the yield of pullulan decreased by 41.01%. The supple...
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: Fei Liu Jinhua Zhang Linjun Zhang Mengqi Diao Peixue Ling Fengshan Wang Source Type: research

pH-sensitive and antibacterial films developed by incorporating anthocyanins extracted from purple potato or roselle into chitosan/polyvinyl alcohol/nano-ZnO matrix: Comparative study
Int J Biol Macromol. 2021 Feb 17:S0141-8130(21)00394-9. doi: 10.1016/j.ijbiomac.2021.02.115. Online ahead of print.ABSTRACTpH-sensitive and antibacterial films based on chitosan/polyvinyl alcohol/nano-ZnO (CPZ) containing anthocyanins extracted from purple potato (PPE) or roselle (RE) were developed. When incorporated with PPE or RE, the moisture contents and flexibility of film significantly reduced (P
Source: International Journal of Biological Macromolecules - February 20, 2021 Category: Biochemistry Authors: Jialin Liu Jiayin Huang Yubin Ying Lingping Hu Yaqin Hu Source Type: research

Corrigendum to "Development of antioxidant chitosan film with banana peels extract and its application as coating in maintaining the storage quality of apple" [Int. J. Biol. Macromol. 154(2020) 1205-1214]
Int J Biol Macromol. 2021 Feb 15:S0141-8130(21)00159-8. doi: 10.1016/j.ijbiomac.2021.01.127. Online ahead of print.NO ABSTRACTPMID:33602499 | DOI:10.1016/j.ijbiomac.2021.01.127 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Wanli Zhang Xiangxin Li Weibo Jiang Source Type: research

Improvement of polyhydroxybutyrate (PHB) plate-based screening method for PHB degrading bacteria using cell-grown amorphous PHB and recovered by sodium dodecyl sulfate (SDS)
Int J Biol Macromol. 2021 Feb 16:S0141-8130(21)00377-9. doi: 10.1016/j.ijbiomac.2021.02.098. Online ahead of print.ABSTRACTPoly(3-hydroxybutyrate) (PHB) is a biobased and biodegradable plastic. Considering the environmental issues of petroleum-based plastics, PHB is promising as it can be degraded in a relatively short time by bacteria to water and carbon dioxide. Substantial efforts have been made to identify PHB-degrading bacteria. To identify PHB-degrading bacteria, solid-based growth or clear zone assays using PHB as the sole carbon source are the easiest methods; however, PHB is difficult to dissolve and distribute ev...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Sol Lee Park Jang Yeon Cho Tae-Rim Choi Hun-Suk Song Shashi Kant Bhatia Ranjit Gurav See-Hyung Park Kyungmoon Park Jeong Chan Joo Sung Yeon Hwang Yung-Hun Yang Source Type: research

Heat-induced self-assembling of BSA at the isoelectric point
Int J Biol Macromol. 2021 Feb 16:S0141-8130(21)00391-3. doi: 10.1016/j.ijbiomac.2021.02.112. Online ahead of print.ABSTRACTMaterials based on ordered protein aggregates have recently received a lot of attention for their application as drug carriers, due to their biocompatibility and their ability to sequester many biological fluids. Bovine serum albumin (BSA) is a good candidate for this use due to its high availability and tendency to aggregate and gel under acidic conditions. In the present work, we employ spectroscopic techniques to investigate the heat-induced BSA aggregation at the molecular scale, in the 12-84 °...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Lucia Comez Pier Luigi Gentili Marco Paolantoni Alessandro Paciaroni Paola Sassi Source Type: research

Antimicrobial activities of chitosan/titanium dioxide composites as a biological nanolayer for food preservation: A review
Int J Biol Macromol. 2021 Feb 16;176:530-539. doi: 10.1016/j.ijbiomac.2021.02.099. Online ahead of print.ABSTRACTPackaging is an integral part of food industry that preserves the properties of food during storage. Food spoilage caused by foodborne microorganisms is a public health problem that imposes a significant burden on the healthcare systems. Moreover, packaging based on artificial and chemical materials such as plastic is destructive to the environment. Chitosan can be categorized as an active food packaging material because of its inherent antimicrobial properties and capacity to carry various active components. Co...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Mohammad Mesgari Amir Hossein Aalami Amirhossein Sahebkar Source Type: research

Underscoring the immense potential of chitosan in fighting a wide spectrum of viruses: A plausible molecule against SARS-CoV-2?
Int J Biol Macromol. 2021 Feb 16:S0141-8130(21)00369-X. doi: 10.1016/j.ijbiomac.2021.02.090. Online ahead of print.ABSTRACTChitosan is a deacetylated polycationic polysaccharide derived from chitin. It is structurally constituted of N-acetyl-D-glucosamine and β-(1-4)-linked D-glucosamine where acetyl groups are randomly distributed across the polymer. The parameters of deacetylation and depolymerization process greatly influence various physico-chemical properties of chitosan and thus, offer a great degree of manipulation to synthesize chitosan of interest for various industrial and biomedical applications. Chitosan a...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Nivya Sharma Chandrima Modak Pankaj Kumar Singh Rahul Kumar Dharmender Khatri Shashi Bala Singh Source Type: research

Characterization of undegraded and degraded silk fibroin and its significant impact on the properties of the resulting silk biomaterials
In this study, we prepared undegraded SF (uSF) and degraded SF (dSF) by common regeneration methods and studied their difference in detail. The results demonstrated that the degradation degree of SF peptide chain had little influence on the secondary structure and thermal stability of SF materials. While, uSF solution showed higher viscosity and surface tension than dSF solution. The uSF membrane (uSFM) could be elongated approximately 134%, 1.6 times the degraded SF membrane (dSFM). SEM implied that both uSF and dSF existed in aqueous solution as micelles with a diameter of approximately 30 nm. dSF could directly form SF ...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Hai-Yan Wang Yu-Qing Zhang Zheng-Guo Wei Source Type: research

Polysaccharides-based bio-nanostructures and their potential food applications
Int J Biol Macromol. 2021 Feb 17;176:540-557. doi: 10.1016/j.ijbiomac.2021.02.107. Online ahead of print.ABSTRACTPolysaccharides are omnipresent biomolecules that hold great potential as promising biomaterials for a myriad of applications in various biotechnological and industrial sectors. The presence of diverse functional groups renders them tailorable functionalities for preparing a multitude of novel bio-nanostructures. Further, they are biocompatible and biodegradable, hence, considered as environmentally friendly biopolymers. Application of nanotechnology in food science has shown many advantages in improving food qu...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Muhammad Bilal Ijaz Gul Aneela Basharat Sarmad Ahmad Qamar Source Type: research

Oriented immobilization of antibodies through different surface regions containing amino groups: Selective immobilization through the bottom of the Fc region
Int J Biol Macromol. 2021 Feb 16:S0141-8130(21)00382-2. doi: 10.1016/j.ijbiomac.2021.02.103. Online ahead of print.ABSTRACTAmino groups on the antibody surface (amino terminus and Lys) are very interesting conjugation targets due to their substantial quantities and selectivity toward various reactive groups. Oriented immobilization of antibodies via amino moieties on the Fc region instead of the antigen-binding fragment (Fab) is highly appreciated to conserve antigen-binding capacity. In this paper, targeting amino moieties on distinct regions, three antibody immobilization strategies were compared with the recognition abi...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Shipeng Gao Francisco Rojas-Vega Javier Rocha-Martin Jos é M Guisán Source Type: research

Hydroxy citric acid cross-linked chitosan/guar gum/poly(vinyl alcohol) active films for food packaging applications
Int J Biol Macromol. 2021 Feb 16:S0141-8130(21)00388-3. doi: 10.1016/j.ijbiomac.2021.02.109. Online ahead of print.ABSTRACTThe present work aims to prepare Chitosan (CS)/Guar gum (GG)/Poly(vinyl alcohol) (PVA) cross-linked with Hydroxy citric acid (HCA) (CGPH active film) by solvent casting technique. The influence of HCA on different CS/PVA ratio (1:3, 1:1, 3:1) in presence of the fixed amount of GG (0.2%) was investigated. The analysis of the results showed that the addition of HCA to the different ratio of CS/PVA increased the degradation temperature and improved the mechanical properties of CGPH active films. FTIR spec...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Veena G Bhat Shivayogi S Narasagoudr Saraswati P Masti Ravindra B Chougale Yogesh Shanbhag Source Type: research

Disaggregation mechanism of prion amyloid for tweezer inhibitor
Int J Biol Macromol. 2021 Feb 16;176:510-519. doi: 10.1016/j.ijbiomac.2021.02.094. Online ahead of print.ABSTRACTThe aggregation of amyloid has been an important event in the pathology of amyloidogenicity. A number of small molecules have been designed for Amyloidosis treatment. Molecular tweezer CLR01, a potential drug for misfolded β-amyloids inhibition, was reportedly bind directly to Lysine residues and interrupt oligomerization. However, the disaggregation mechanism of amyloid for this inhibitor is unclear. Here we used long timescale of molecular dynamic simulation to reveal the mechanism of disaggregation for p...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Mueed Ur Rahman Ashfaq Ur Rehman Taaha Arshad Hai-Feng Chen Source Type: research

A robust regenerated cellulose-based dual stimuli-responsive hydrogel as an intelligent switch for controlled drug delivery
Int J Biol Macromol. 2021 Feb 17;176:448-458. doi: 10.1016/j.ijbiomac.2021.02.104. Online ahead of print.ABSTRACTConstructing robust hydrogels with biodegradability and dual stimuli-responsive by utilizing natural polymer as raw materials remains a sustaining challenge. Herein, we proposed an interpenetrating strategy in which N-isopropyl acrylamide (NIPAM) and acrylamide (AM) block copolymers were introduced as the second network into the carboxymethyl cellulose single network gel (CMC gel) to construct a dual-network robust hydrogel (CMC/PNIPAM-co-PAM). The dual-network design strategy effectively improves the mechanical...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Fuyu Song Jingwei Gong Yehan Tao Yi Cheng Jie Lu Haisong Wang Source Type: research

Ninhydrin-functionalized chitosan for selective removal of Pb(II) ions: Characterization and adsorption performance
Int J Biol Macromol. 2021 Feb 16:S0141-8130(21)00389-5. doi: 10.1016/j.ijbiomac.2021.02.110. Online ahead of print.ABSTRACTA chitosan-based adsorbents (CS-Ninhydrin) was prepared by grafting ninhydrin for Pb(II) ions adsorption. SEM-EDS, XRD and FTIR analysis were used to characterize the synthesized CS-Ninhydrin. The static adsorption experiments showed that CS-Ninhydrin had a good removal rate for Pb(II) ions in a wide range of pH 3 to 7, quickly reached equilibrium (120 min) and had a higher adsorption capacity (196 mg/g). Pseudo second-order and Langmuir models showed that the adsorption process of Pb(II) by CS-Ninhydr...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Yingbi Chen Jiali Tang Shixing Wang Libo Zhang Source Type: research

Development of a naftopidil-chitosan-based fumaric acid solid dispersion to improve the dissolution rate and stability of naftopidil
Int J Biol Macromol. 2021 Feb 17;176:520-529. doi: 10.1016/j.ijbiomac.2021.02.096. Online ahead of print.ABSTRACTNaftopidil (NAF), an α1-adrenoceptor antagonist, is administered as a treatment for benign prostatic hyperplasia; however, according to the Biopharmaceutical Classification System (BCS IV), it is a poorly-soluble drug that exhibits poor permeability. We aimed to increase the dissolution (%) of NAF by adding chitosan to a polymer-free formulation. Compared to the formulation prepared using Flivas®, at 60 min, the solid dispersion (SD) formulation containing NAF, fumaric acid, chitosan, and US2® in a...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Jeong Sun Sohn Jae-Seon Kim Jin-Seok Choi Source Type: research

New deep eutectic solvent assisted extraction of highly pure lignin from maritime pine sawdust (Pinus pinaster)
Int J Biol Macromol. 2021 Feb 16:S0141-8130(21)00367-6. doi: 10.1016/j.ijbiomac.2021.02.088. Online ahead of print.ABSTRACTLignocellulosic biomass is a renewable and sustainable feedstock, mainly composed of cellulose, hemicellulose, and lignin. Lignin, as the most abundant natural aromatic polymer occurring on Earth, has great potential to produce value-added products. However, the isolation of highly pure lignin from biomass requires the use of efficient methods during lignocellulose fractionation. Therefore, in this work, novel acidic deep eutectic solvents (DES) were prepared, characterized and screened for lignin extr...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Catarina Fernandes Elodie Melro Solange Magalh ães Lu ís Alves Rita Craveiro Alexandra Filipe Artur J M Valente Gabriela Martins Filipe E Antunes Anabela Romano Bruno Medronho Source Type: research

Protecting thermodynamic stability of protein: The basic paradigm against stress and unfolded protein response by osmolytes
Int J Biol Macromol. 2021 Feb 16:S0141-8130(21)00381-0. doi: 10.1016/j.ijbiomac.2021.02.102. Online ahead of print.ABSTRACTOrganic osmolytes are known to play important role in stress protection by stabilizing macromolecules and suppressing harmful effects on functional activity. There is existence of several reports in the literature regarding their effects on structural, functional and thermodynamic aspects of many enzymes and the interaction parameters with proteins have been explored. Osmolytes are compatible with enzyme function and therefore, can be accumulated up to several millimolar concentrations. From the thermo...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Gurumayum Suraj Sharma Snigdha Krishna Sheeza Khan Tanveer A Dar Khurshid A Khan Laishram Rajendrakumar Singh Source Type: research

Low molecular weight chitosan-cyanocobalamin nanoparticles for controlled delivery of ciprofloxacin: Preparation and evaluation
This study was carried out to project a safe nano-drug carrier composed of chitosan and cyanocobalamin (CNCbl) to improve oral delivery of ciprofloxacin hydrochloride (CIP). CIP is classified in class IV of the biopharmaceutical classification system with low solubility and permeabilityA, so it has some problems if given orally. Novel conjugate of low molecular weight chitosan, as a natural biopolymer, and CNCbl was synthesized, and then drug loading and in-vitro drug release were assessed. The loading of CIP was optimized by the Design-Expert software and the central composite design method, and that the optimal drug load...
Source: International Journal of Biological Macromolecules - February 19, 2021 Category: Biochemistry Authors: Nafiseh Hosseini-Ashtiani Azadeh Tadjarodi Rouholah Zare-Dorabei Source Type: research

Facile synthesis of Zr(IV)-crosslinked carboxymethyl cellulose/carboxymethyl chitosan hydrogel using PEG as pore-forming agent for enhanced phosphate removal.
Abstract Zr(IV)-crosslinked carboxymethyl cellulose/carboxymethyl chitosan hydrogels were prepared using polyethylene glycol (PEG) as pore-forming agent. The hydrogels exhibited porous structure and the pore size increased with the PEG content. The obtained hydrogels were used as adsorbents for removal of phosphate from aqueous solutions. The hydrogel prepared with most amount of PEG displayed largest adsorption amount of phosphate. This result might be attributed to improvement of surface area and pore volume caused by introduction of PEG, which could boost exposure of adsorption sites in the hydrogel and enhance...
Source: International Journal of Biological Macromolecules - February 17, 2021 Category: Biochemistry Authors: Wang J, Wei J Tags: Int J Biol Macromol Source Type: research

Biophysical interaction between self-assembled branched DNA nanostructures with bovine serum albumin and bovine liver catalase.
Abstract Branched DNA (bDNA) nanostructures have emerged as self-assembled biomaterials and are being considered for biomedical applications. Herein, we report the biophysical interaction between self-assembled bDNA nanostructure with circulating protein bovine serum albumin (BSA) and cellular enzyme bovine liver catalase (BLC). The binding between bDNA and BSA or BLC was confirmed through the decrease in fluorescence spectra. The Stern-Volmer data supports for non-covalent bonding with ~1 binding site in case of BSA and BLC thus advocating a static binding. Furthermore, FTIR and ITC study confirmed the binding of...
Source: International Journal of Biological Macromolecules - February 17, 2021 Category: Biochemistry Authors: Baral B, Dutta J, Subudhi U Tags: Int J Biol Macromol Source Type: research