Fractionation of lignin using organic solvents: A combined experimental and theoretical study.
Abstract Refining of industrial lignin to produce homogeneous fractions is essential for high-value applications. However, the understanding of key interactions between a variety of solvents with lignin polymer is still uncertain. In this work, single-step fractionation of industrial hardwood kraft lignin (HKL) using organic solvents of different polarities - ethanol, acetone, diethyl ether and hexane - was investigated by combining an experimental and theoretical approach. Experimental results revealed that higher polarity solvents (ethanol and acetone) exhibited higher solubility yield compared to moderate and l...
Source: International Journal of Biological Macromolecules - November 23, 2020 Category: Biochemistry Authors: Ponnuchamy V, Gordobil O, Diaz RH, Sandak A, Sandak J Tags: Int J Biol Macromol Source Type: research

Characterization of ligno-cellulosic fiber extracted from Atriplex halimus L. plant.
Abstract Lignocellulosic fiber extracted from saltbush (Atriplex halimus L.) is characterized as reinforcement of composite materials. The morphological, physical, thermal and mechanical properties of fibers were addressed for the first time in this paper. The fibers were also subjected to chemical analysis. Stems were boiled in 0.5% sodium hydroxide (NaOH) or 10% sodium bicarbonate (NaHCO3). Optical and scanning electron microscopy images show an abundance of fiber in the form of thick-walled polygonal tubes. NaOH treatment yielded rough-surfaced fibers whereas the NaHCO3 treatment yielded smooth-surfaced fiber. ...
Source: International Journal of Biological Macromolecules - November 23, 2020 Category: Biochemistry Authors: Belouadah Z, Toubal L, Belhaneche-Bensemra N, Ati A Tags: Int J Biol Macromol Source Type: research

Preparation of AAEK-functionalized cellulose film with antibacterial and anti-adhesion activities.
Abstract Bacterial adhesion infection caused by medical materials in clinical application has become a serious threat, and it urgently needs new strategies to deal with these clinical challenges. The purpose of this study is to explore the effectiveness of surface-decorated aryl (β-amino) ethyl ketones (AAEK), a promising sorting enzyme A (SrtA) inhibitor of Staphylococcus aureus, to improve the anti-adhesion ability of biomaterials. AAEK was covalently grafted onto cellulose films (CF) via copper-catalyzed azide-alkyne 1, 3-dipolar cycloaddition click reaction. The data of contact angle measurements, ATR-FTI...
Source: International Journal of Biological Macromolecules - November 23, 2020 Category: Biochemistry Authors: Zhou Z, Wang L, Hu Y, Song R, Mei N, Chen T, Tang S Tags: Int J Biol Macromol Source Type: research

Strategies to expand the therapeutic potential of superoxide dismutase by exploiting delivery approaches.
Abstract The overproduction of free radicals can cause oxidative-stress damage to a range of biomolecules, and thus potentially contribute to several pathologies, from neurodegenerative disorders to cardiovascular diseases and metabolic disorders. Endogenous antioxidant enzymes, such as superoxide dismutase (SOD), play an important role in diminishing oxidative stress. SOD supplementation could therefore be an effective preventive strategy to reduce the risk of free-radical overproduction. However, the efficacy of SOD administration is hampered by its rapid clearance. Several different approaches to improve the bi...
Source: International Journal of Biological Macromolecules - November 23, 2020 Category: Biochemistry Authors: Rosa AC, Bruni N, Meineri G, Corsi D, Calvi N, Gastaldi D, Dosio F Tags: Int J Biol Macromol Source Type: research

Bioengineered chitosan-magnesium nanocomposite: A novel agricultural antimicrobial agent against Acidovorax oryzae and Rhizoctonia solani for sustainable rice production.
In this study, we reported the green synthesis of chitosan-magnesium (CS-Mg) nanocomposite and its antimicrobial activity against two rice pathogens namely Acidovorax oryzae and Rhizoctonia solani for the first time. The green MgO nanoparticles synthesized by using a native Bacillus sp. strain RNT3, were used to fabricate CS-Mg nanocomposite utilizing one-pot synthesis method. The synthesis of CS-Mg nanocomposite was further confirmed by using UV-vis spectroscopy, whereas, FTIR and XRD analysis showed the capping of CS-Mg nanocomposites by different functional groups together with their crystalline structure, respectively....
Source: International Journal of Biological Macromolecules - November 23, 2020 Category: Biochemistry Authors: Ahmed T, Noman M, Luo J, Muhammad S, Shahid M, Ali MA, Zhang M, Li B Tags: Int J Biol Macromol Source Type: research

A comparative study on the leishmanicidal activity of the L-amino acid oxidases BjussuLAAO-II and BmooLAAO-II isolated from Brazilian Bothrops snake venoms.
This study aims to examine whether two L-amino acid oxidases isolated from Bothrops snake venom (SV-LAAOs) were cytotoxic to Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis, two causative agents of leishmaniasis, which is an endemic disease in tropical and subtropical countries. The SV-LAAOs BjussuLAAO-II and BmooLAAO-II were isolated from Bothrops jararacussu and Bothrops moojeni venom, respectively, through a three-step chromatography process that used molecular exclusion, hydrophobic interaction, and affinity columns. BmooLAAO-II is a new SV-LAAO isoform that we isolated in this study. The puri...
Source: International Journal of Biological Macromolecules - November 23, 2020 Category: Biochemistry Authors: Barbosa LG, Costa TR, Borges IP, Costa MS, Carneiro AC, Borges BC, Silva MJB, Amorim FG, Quinton L, Yoneyama KAG, de Melo Rodrigues V, Sampaio SV, Rodrigues RS Tags: Int J Biol Macromol Source Type: research

Development and optimization of Fe3+-crosslinked sodium alginate-methylcellulose semi-interpenetrating polymer network beads for controlled release of ibuprofen.
In this study, ionically crosslinked beads of sodium alginate (NaAlg) and methylcellulose (MC) were prepared as semi-interpenetrating polymer networks (semi-IPN) in the size range of 1.97 ± 0.09-1.22 ± 0.13 mm by crosslinking with FeCl3 and loaded with ibuprofen (IBU), which is a nonsteroidal anti-inflammatory drug. The highest 93.33% entrapment efficiency of IBU was achieved, and the drug release behaviors, mean particle size, and entrapment efficiency of beads were investigated in terms of the polymer composition, a ratio of ibuprofen to polymer, exposure time to crosslinker, and concentration of ...
Source: International Journal of Biological Macromolecules - November 23, 2020 Category: Biochemistry Authors: Bulut E Tags: Int J Biol Macromol Source Type: research

Effect of hydrothermal treatment on physicochemical properties of amorphous granular potato starch (AGPS).
Abstract Using restructuring technology, A- or B-type crystalline granular potato starch was produced from amorphous granular potato starch (AGPS). AGPS was prepared using ethanol-heat processing, and hydrothermal treatments were performed with different moisture contents (18, 29, 200% d.b.) and temperatures (4, 25, 40, 60, 80 °C) for 3 weeks. AGPS showed no endothermic peak in a DSC thermogram, while hydrothermally treated AGPS (HAGPS) revealed endothermic peaks. In X-ray diffraction, AGPS displayed an amorphous pattern, and HAGPS displayed A- or B-type crystalline patterns depending on treatment temperat...
Source: International Journal of Biological Macromolecules - November 23, 2020 Category: Biochemistry Authors: Yeum JH, Choi HW, Kim HY, Oh SM, Bae JE, Ye SJ, Baik MY Tags: Int J Biol Macromol Source Type: research

In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.
Abstract The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota. Results showed that the contents of reducing sugar of LLP slightly increased during the gastrointestinal digestion, and its molecular weight also slightly decreased, suggesting that LLP could be slightly degraded under the gastrointestinal digestion conditions. Moreover, during the fecal fermentation, the molecular weight of the indigestib...
Source: International Journal of Biological Macromolecules - November 21, 2020 Category: Biochemistry Authors: Wu DT, Fu Y, Guo H, Yuan Q, Nie XR, Wang SP, Gan RY Tags: Int J Biol Macromol Source Type: research

Immobilization of enzymes on nanoinorganic support materials: An update.
Abstract Despite the widespread use in various industries, enzyme's instability and non-reusability limit their applications which can be overcome by immobilization. The nature of the enzyme's support material and method of immobilization affect activity, stability, and kinetics properties of enzymes. Here, we report a comparative study of the effects of inorganic support materials on immobilized enzymes. Accordingly, immobilization of enzymes on nanoinorganic support materials significantly improved thermal and pH stability. Furthermore, immobilizations of enzymes on the materials mainly increased Km values while...
Source: International Journal of Biological Macromolecules - November 21, 2020 Category: Biochemistry Authors: Ashkan Z, Hemmati R, Homaei A, Dinari A, Jamlidoost M, Tashakor A Tags: Int J Biol Macromol Source Type: research

Inside-outside Ag nanoparticles-loaded polylactic acid electrospun fiber for long-term antibacterial and bone regeneration.
In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLL...
Source: International Journal of Biological Macromolecules - November 21, 2020 Category: Biochemistry Authors: Liu F, Cheng X, Xiao L, Wang Q, Yan K, Su Z, Wang L, Ma C, Wang Y Tags: Int J Biol Macromol Source Type: research

Xyloglucan-based hybrid nanocomposite with potential for biomedical applications.
NMPS Abstract Natural polymer-based hybrid nanocomposites have been proposed as one of the most promising tools for biomedical applications, including disease treatment and diagnosis procedures. Xyloglucan nanocapsules can simultaneously load magnetic iron oxide nanoparticles and bioactive for a specific tissue, reducing the processes of degradation and metabolic inactivation of molecules with biological activity. In this work, magnetic nanocapsules of xyloglucan loaded with hydrophilic sulfated quercetin (MNXQ_SO3) were successfully synthesized by inverse miniemulsion process through interfacial polymerization. ...
Source: International Journal of Biological Macromolecules - November 21, 2020 Category: Biochemistry Authors: da Silva ACC, de Almeida RR, da Cruz Sousa AC, Martínez FNA, Denardin JC, de Morais SM, Ricardo NMPS Tags: Int J Biol Macromol Source Type: research

Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment.
Abstract A novel, sustainable chitosan polymeric nanocomposite (CS-PVA@CuO) was synthesized and subjected to the removal of acid blue 25 (AB25) from the aqueous environment. The influence of different variables such as pH, contact time, initial dye concentration, temperature, and adsorption kinetics has been examined in the batch adsorption process. The CS-PVA@CuO composite was systematically characterized by XRD, FTIR, SEM, and EDX analysis. The pseudo-first order (PFO), pseudo-second order (PSO), and intra-particle diffusion kinetics equations were used to examine the kinetic data of the adsorption process. The ...
Source: International Journal of Biological Macromolecules - November 21, 2020 Category: Biochemistry Authors: Krishna LS, Soontarapa K, Vinaykumar, Marella RK, Karthik K Tags: Int J Biol Macromol Source Type: research

Fabrication of hybrid chitosan encapsulated magnetic-kaolin beads for adsorption of phosphate and nitrate ions from aqueous solutions.
Abstract Phosphate and nitrate are commonly used industrial and agricultural nutrients that are of great anxiety because of their ubiquitous existence in water and wastewater sources and association with harmful health effects. Herein, we aimed to fabricate a novel and environmental-friendly chitosan encapsulated magnetic kaolin beads for the first time and applied for the adsorption of phosphate and nitrate ions from water. The physico-chemical properties of MK-chitosan beads were established by XRD, FTIR, and TGA-DSC techniques. Surface area (BET) analysis shows that MK-chitosan beads have a specific surface are...
Source: International Journal of Biological Macromolecules - November 21, 2020 Category: Biochemistry Authors: Karthikeyan P, Meenakshi S Tags: Int J Biol Macromol Source Type: research

Structural features and antioxidant activity of a new galactoglucan from edible mushroom Pleurotus djamor.
Abstract A new water soluble galactoglucan with apparent molecular weight ~1.61 × 105 Da, was isolated from the edible mushroom Pleurotus djamor by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography. The sugar analysis showed the presence of glucose and galactose in a molar ratio of nearly 3:1 respectively. The structure of the repeating unit in the polysaccharide was determined through chemical and NMR experiments as: In vitro antioxidant studies showed that the PDPS exhibited hydroxyl radical scavenging activity (EC50 = ...
Source: International Journal of Biological Macromolecules - November 21, 2020 Category: Biochemistry Authors: Maity GN, Maity P, Khatua S, Acharya K, Dalai S, Mondal S Tags: Int J Biol Macromol Source Type: research

Physicochemical properties and in vitro digestibility of proso millet starch after addition of Proanthocyanidins.
Abstract The solubility and swelling power, pasting, structure, and digestibility properties of proso millet starch (PMS) with different concentration of proanthocyanidins (PA) were investigated. The results showed that with PA content increasing, the solubility and swelling power of starch increased, the PA accelerated the water absorption and expansion of starch. The results of pasting properties showed that PA increased the peak viscosity of starch, but decreased the setback and pasting temperature. A significant decrease in enthalpy value was measured by differential scanning calorimetry, the enthalpy value de...
Source: International Journal of Biological Macromolecules - November 20, 2020 Category: Biochemistry Authors: Xiao Y, Zheng M, Yang S, Li Z, Liu M, Yang X, Lin N, Liu J Tags: Int J Biol Macromol Source Type: research

Molecular complexes of calf thymus DNA with various bioactive compounds: Formation and characterization.
Abstract The interaction between biomacromolecules and ligands has attracted great interest because of their biological properties. Calf thymus DNA (ctDNA) can interact with bioactive compounds to form complexes. Here, ctDNA-ligand complexes were studied using fluorescence, absorption, and infrared spectroscopy, circular dichroism, ABTS assay and competitive displacement. The binding constants of bioactive compounds at the intercalative site of ctDNA ranked in order kaempferol> apigenin> quercetin> curcumin> riboflavin, while the binding constants at minor groove sites ranked quercetin> kaempferol&g...
Source: International Journal of Biological Macromolecules - November 20, 2020 Category: Biochemistry Authors: Ji C, Yin X, Duan H, Liang L Tags: Int J Biol Macromol Source Type: research

Triphenylphosphonium-conjugated glycol chitosan microspheres for mitochondria-targeted drug delivery.
Abstract To develop an efficient vector for mitochondria-targeted drug delivery, we synthesized triphenylphosphonium (TPP)-modified glycol chitosan polymeric microspheres that had a unique chemical structure with both lipophilic phenyl groups and cationic phosphonium. Notably, TPP can easily pass through the phospholipid bilayer of mitochondria, thereby resulting in specific accumulation of a combined drug molecule in the mitochondria due to the membrane potential between TPP and its membrane. Therefore, TPP has been widely used as a mitochondria-targeting moiety. Triphenylphosphonium-glycol chitosan derivatives (...
Source: International Journal of Biological Macromolecules - November 20, 2020 Category: Biochemistry Authors: Lee YH, In Park H, Chang WS, Choi JS Tags: Int J Biol Macromol Source Type: research

A novel serine protease with anticoagulant and fibrinolytic activities from the fruiting bodies of mushroom Agrocybe aegerita.
Abstract A novel fibrinolytic enzyme, ACase was isolated from fruiting bodies of a mushroom, Agrocybe aegerita. ACase was purified by using ammonium sulfate precipitation, gel filtration, ion exchange and hydrophobic chromatographies to 237.12 fold with a specific activity of 1716.77 U/mg. ACase was found to be a heterodimer with molecular mass of 31.4 and 21.2 kDa by SDS-PAGE and appeared as a single band on Native-PAGE and fibrin-zymogram. The N-terminal sequence of the two subunits of ACase was AIVTQTNAPWGL (subunit 1) and SNADGNGHGTHV (subunit 2). ACase had maximal activity at 47 °C and pH 7.6. It'...
Source: International Journal of Biological Macromolecules - November 20, 2020 Category: Biochemistry Authors: Li G, Liu X, Cong S, Deng Y, Zheng X Tags: Int J Biol Macromol Source Type: research

Inhibition of HIF-1 α/EP4 axis by hyaluronate-trimethyl chitosan-SPION nanoparticles markedly suppresses the growth and development of cancer cells.
In this study, we used hyaluronate (HA), and trimethyl chitosan (TMC) recoated superparamagnetic iron oxide nanoparticles (SPIONs) loaded with HIF-1α-silencing siRNA and the EP4 antagonist (E7046) to treat cancer cells and assessed the effect of combination therapy on cancer progression. The results showed that optimum physicochemical characteristics of NPs (size 126.9 nm, zeta potential 27 mV, PDI 
Source: International Journal of Biological Macromolecules - November 20, 2020 Category: Biochemistry Authors: Karpisheh V, Afjedi JF, Afjadi MN, Haeri MS, Sough TSA, Asl SH, Edalati M, Atyabi F, Masjedi A, Hajizadeh F, Izadi S, Tekie FSM, Hajiramezanali M, Sojoodi M, Jadidi-Niaragh F Tags: Int J Biol Macromol Source Type: research

Synthesis of chitosan nanocomposites for controlled release applications.
Abstract Chitosan (CS) was modified using hydroxyapatite (HA) and multiwalled carbon nanotubes (MWCNT) followed by crosslinking with glutaraldehyde (GA). The obtained products were characterized and investigated with thermal analysis. The modified CS suffered a slight weight loss % up to 240 °C then extensive weight loss (EWL)% up to 420 °C and a slight weight loss again until the end of measurement at 700 °C. The treatment showed more thermal stability of modified CS over the blank CS. The 20% HA modified CS showed the highest thermal stability among CS/HA composites while adding CNT to the matr...
Source: International Journal of Biological Macromolecules - November 20, 2020 Category: Biochemistry Authors: Al Shanqiti EM, Alfooty KO, Abdelaal MY Tags: Int J Biol Macromol Source Type: research

Polydopamine-assisted grafting of chitosan on porous poly (L-lactic acid) electrospun membranes for adsorption of heavy metal ions.
In this study, a versatile method for the manufacturing of chitosan-grafted porous poly (L-lactic acid) (P-PLLA) nanofibrous membrane by using polydopamine (PDA) as an intermediate layer has been developed. P-PLLA fibres were electrospun and collected as nano/micro fibrous membranes. Highly porous fibres could serve as a substrate for chitosan to adsorb heavy metal ions. Moreover, PDA was used to modify P-PLLA surface to increase the coating uniformity and stability of chitosan. Due to the very high surface area of P-PLLA membranes and abundant amine groups of both PDA and chitosan, the fabricated membranes were utilized a...
Source: International Journal of Biological Macromolecules - November 19, 2020 Category: Biochemistry Authors: Zia Q, Tabassum M, Meng J, Xin Z, Gong H, Li J Tags: Int J Biol Macromol Source Type: research

Development of pullulanase mutants to enhance starch substrate utilization for efficient production of β-CD.
Development of pullulanase mutants to enhance starch substrate utilization for efficient production of β-CD. Int J Biol Macromol. 2020 Nov 18;: Authors: Li X, Ji H, Bai Y, Jin Z Abstract The inhibitory effect of β-CD on pullulanase which hydrolyzes α-1,6 glycosidic bond in starch to release more available linear substrates, limited substrate utilization thus influencing the yield of β-CD. Here, an aspartic acid residue (D465) which interacted with cyclodextrin ligand by hydrogen bond, was mutated to explore its contribution to bind inhibitors and obtain mutants with lower affinity...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Li X, Ji H, Bai Y, Jin Z Tags: Int J Biol Macromol Source Type: research

Influence of moisture and amylose on the physicochemical properties of rice starch during heat treatment.
Abstract Moisture and amylose are important factors affecting the quality of heat-treated starches. The amylose content in heat-treated rice starch increased as moisture content (MC) increased from 8% to 30%, but decreased at MC of 70%. With the increase of MC, the paste transmittance, gelatinization temperature, and digestibility of starch increased, whereas the swelling power and enthalpy decreased. The long- and short-range molecular order and the digestive properties of starch with MC ≤ 30% changed moderately, but high MC (70%) gelatinized the starch and drastically changed the physicochemical propertie...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Yang Z, Hao H, Wu Y, Liu Y, Ouyang J Tags: Int J Biol Macromol Source Type: research

Properties and potential applications of mannuronan C5-epimerase: A biotechnological tool for modifying alginate.
Abstract Given the excellent characteristics of alginate, it is an industrially important polysaccharide. Mannuronan C5-epimerase (MC5E) is an alginate-modifying enzyme that catalyzes the conversion of β-D-mannuronate (M) to its C5 epimer α-L-guluronate (G) in alginate. Both the biological activities and physical properties of alginate are determined by M/G ratios and distribution patterns. Therefore, MC5E is regarded as a biotechnological tool for modifying and processing alginate. Various MC5Es derived from brown algae, Pseudomonas and Azotobacter have been isolated and characterized. With the rapid d...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Ci F, Jiang H, Zhang Z, Mao X Tags: Int J Biol Macromol Source Type: research

Structural studies of immunomodulatory (1  → 3)-, (1 → 4)-α glucan from an edible mushroom Polyporus grammocephalus.
Structural studies of immunomodulatory (1 → 3)-, (1 → 4)-α glucan from an edible mushroom Polyporus grammocephalus. Int J Biol Macromol. 2020 Nov 18;: Authors: Patra S, Maity P, Chakraborty I, Sen IK, Ghosh D, Rout D, Bhanja SK Abstract A water soluble polysaccharide (PGPS) with molecular weight ~ 1.4 × 105 Da was isolated by alkali treatment from an edible mushroom Polyporus grammocephalus and purified by gel chromatography using sepharose-6B column. Monosaccharide analysis revealed that PGPS was made up of glucose only. PGPS contained (1 → 3)-α-...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Patra S, Maity P, Chakraborty I, Sen IK, Ghosh D, Rout D, Bhanja SK Tags: Int J Biol Macromol Source Type: research

Antioxidant and antibacterial polyelectrolyte wound dressing based on chitosan/hyaluronan/phosphatidylcholine dihydroquercetin.
on;oltés L Abstract Antioxidant and antimicrobial wound dressings are the most favorable for acute and chronic wounds treatment. Herein, we formulated a multifunctional polyelectrolyte wound dressing membrane on the basis of chitosan (Ch) and hyaluronan (HA) enhanced by phosphatidylcholine dihydroquercetin (PCDQ). Physicochemical properties and microstructures of fabricated films were investigated adopting Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA) and scanning electron microscope (SEM). Furthermore, water uptakes, wettability profiles, surface roughness, and mechanica...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Hassan MA, Tamer TM, Valachová K, Omer AM, El-Shafeey M, Mohy Eldin MS, Šoltés L Tags: Int J Biol Macromol Source Type: research

A new lectin from the floral capitula of Egletes viscosa (EgviL): Biochemical and biophysical characterization and cytotoxicity to human cancer cells.
This study aimed to purify, characterize, and evaluate the cytotoxicity of a lectin (EgviL) from the floral capitula of E. viscosa. The lectin was isolated from saline extract through precipitation with ammonium sulfate followed by Sephadex G-75 chromatography. The molecular mass and isoelectric point (pI) of EgviL were determined as well as its temperature and pH stability. Physical-chemical parameters of interaction between EgviL and carbohydrates were investigated by fluorescence quenching and 1H nuclear magnetic resonance (NMR). Cytotoxicity was investigated against human peripheral blood mononuclear cells (PBMCs) and ...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Gomes DC, Barros MR, Menezes TM, Neves JL, Paiva PMG, da Silva TG, Napoleão TH, Coriolano MC, Dos Santos Correia MT Tags: Int J Biol Macromol Source Type: research

Carboxymethyl chitosan microspheres loaded hyaluronic acid/gelatin hydrogels for controlled drug delivery and the treatment of inflammatory bowel disease.
This study also provides a reference for the design and preparation of a new oral drug delivery system with controlled release behavior. PMID: 33220374 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Zhang S, Kang L, Hu S, Hu J, Fu Y, Hu Y, Yang X Tags: Int J Biol Macromol Source Type: research

Effect of homogenization-pressure-assisted enzymatic hydrolysis on the structural and physicochemical properties of lotus-seed starch nanoparticles.
Abstract In previous studies, we successfully prepared lotus-seed starch nanoparticles (LS-SNPs) using enzymatic methods. To further improve their performance, we studied the structural, physical and chemical properties of LS-SNPs prepared by high-pressure homogenization (HPH)-assisted enzymatic hydrolysis (EH). HPH treatments at different pressures and frequencies have a significant effect on the particle size and molecular weight of LS-SNPs. Structural analyses showed that LS-SNP and H-LS-SNP both comprised B-type starch crystals. As the homogenization pressure and frequency were increased, the relative crystall...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Wang B, Lin X, Zheng Y, Zeng M, Huang M, Guo Z Tags: Int J Biol Macromol Source Type: research

Low molecular weight fucoidan fraction ameliorates inflammation and deterioration of skin barrier in fine-dust stimulated keratinocytes.
Abstract Recently evidence linking the effects of fine-dust (FD) on skin inflammation is exaggerating. Fucoidan derived from brown algae has great potential for ameliorating oxidative stress and inflammation. Herein, a fucoidan fraction (SHC4-6) was purified from an enzymatic (Celluclast) extract of an invasive seaweed, Sargassum horneri following gradient ethanol precipitation and anion exchange chromatography. Effectiveness of SHC4-6 in ameliorating FD (from Beijing, China)-induced inflammatory responses in HaCaT keratinocytes and recovery of skin barrier dysfunction was evaluated. SHC4-6 was comprising of sulfa...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Fernando IPS, Dias MKHM, Madusanka DMD, Han EJ, Kim MJ, Heo SJ, Lee K, Cheong SH, Ahn G Tags: Int J Biol Macromol Source Type: research

Carboxymethyl cellulose nanocomposite beads as super-efficient catalyst for the catalytic reduction of organic and inorganic pollutants.
Abstract Carboxymethyl cellulose/copper oxide-nickel oxide (CMC/CuO-NiO) nanocomposite beads were prepared by facile, simple and environmentally friendly method. Initially, CuO-NiO was prepared and applied for the catalytic reduction of 4-nitrophenol (4-NP). The results showed that CuO-NiO demonstrated high catalytic activity toward the reduction of 4-NP to 4-aminophenol (4-AP) with a rate constant of 2.97 × 10-2 s-1. Further, CuO-NiO were well-dispersed in the polymeric matrix of carboxymethyl cellulose to prepare CMC/CuO-NiO beads. CMC/CuO-NiO nanocomposite beads were also applied to catalyze the red...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Maslamani N, Khan SB, Danish EY, Bakhsh EM, Zakeeruddin SM, Asiri AM Tags: Int J Biol Macromol Source Type: research

Defective chicken skin collagen molecules, hydrolyzed by actinidain protease, assemble to form loosely packed fibrils that promote cell spheroid formation.
Abstract Cells recognize collagen fibrils as the first step in the process of adherence. Fibrils of chicken skin actinidain-hydrolyzed collagen (low adhesive scaffold collagen, LASCol), in which the telopeptide domains are almost completely removed, cause adhering cells to form spheroids instead of adopting a monolayer morphology. Our goal was to elucidate the ultrastructure of the LASCol fibrils compared with pepsin-hydrolyzed collagen (PepCol) fibrils. At low concentrations of 0.2 mg/mL, the time to reach the maximum increasing rate of turbidity for LASCol was all slower than that for PepCol. Differential scan...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Morimoto K, Kunii S, Tonomura B Tags: Int J Biol Macromol Source Type: research

Fabrication of pea protein-curcumin nanocomplexes via microfluidization for improved solubility, nano-dispersibility and heat stability of curcumin: Insight on interaction mechanisms.
This study indicated that PP exhibiting as an emulsifier and carrier might significantly reduce the surface tension of NS and in turns prolong their storability. PMID: 33220379 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Zhang H, Wang T, He F, Chen G Tags: Int J Biol Macromol Source Type: research

Carboxymethylation of (1  → 6)-α-dextran from Leuconostoc spp.: Effects on microstructural, thermal and antioxidant properties.
Carboxymethylation of (1 → 6)-α-dextran from Leuconostoc spp.: Effects on microstructural, thermal and antioxidant properties. Int J Biol Macromol. 2020 Nov 18;: Authors: Li W, Yun L, Rifky M, Liu R, Wu T, Sui W, Zhang M Abstract The carboxymethylated (1 → 6)-α-dextran (CM-dex) was synthesized by introducing carboxymethyl groups at different degrees of substitution (DS). The resulting dex1-1, dex2-1, dex3-1, and dex4-1 products had degrees of substitution of 0.57, 0.78, 1.13, and 1.25, respectively. The dex3-1 showed the highest glass transition temperature (Tg) of 215.96...
Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Li W, Yun L, Rifky M, Liu R, Wu T, Sui W, Zhang M Tags: Int J Biol Macromol Source Type: research

Sulfated polysaccharides from Undaria pinnatifida improved high fat diet-induced metabolic syndrome, gut microbiota dysbiosis and inflammation in BALB/c mice.
This study showed that sulfated polysaccharides from U. pinnatifida (UPSP) reduced weight gain, fat accumulation and metabolic disorders in mice fed with high fat diet (HFD). UPSP not only alleviated HFD-induced microbiota dysbiosis indicated as increased abundances of some Bacteroidales members that had positive correlations with the improvement of physiological indexes, but also maintained gut barrier integrity and reduced metabolic endotoxemia. A dose-effect relationship was observed between the dose of UPSP and its effect on some physiological indexes, gut microbiota community and nutrient utilization. The in vitro res...
Source: International Journal of Biological Macromolecules - November 17, 2020 Category: Biochemistry Authors: Jiang P, Zheng W, Sun X, Jiang G, Wu S, Xu Y, Song S, Ai C Tags: Int J Biol Macromol Source Type: research

Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.
Abstract A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG). Scanning electron microscopy (SEM) showed that FSIE was successfully compounded with CG. Although the addition of FSIE slightly increased the water vapor permeability (WVP) and O2 permeability (OP) of the film, it also improved its ability to block ultraviolet light significantly. The appropriate amounts of FSIE increased the tensile strength (TS) from 20.9 MPa to 30.2 MPa but reduced the elongation at break (EB) from 38.7% to 27.6%. The films do...
Source: International Journal of Biological Macromolecules - November 17, 2020 Category: Biochemistry Authors: Meng F, Zhang C, Li J, Wang L Tags: Int J Biol Macromol Source Type: research

Optimizing the properties of Zodo gum and examining its potential for amino acid binding by periodate oxidation.
In this study, the Zodo gum exudated by Amygdalus scoparia spach underwent the periodate oxidation process for chemical modification and the formation of dialdehyde groups. Modification of the Zodo gum properties was done using the periodate oxidation method, response surface methodology (RSM) and central composite design (CCD), with 4 factors of sodium periodate volume (6.4-19.2 ml), temperature (35-55 °C), pH (3-5) and time (2-4 h). Dialdehyde Zodo gum (DZG) was produced by controlling test variables and measuring some responses including dialdehyde content and efficacy, in addition to evaluating the rheologica...
Source: International Journal of Biological Macromolecules - November 17, 2020 Category: Biochemistry Authors: Mozafari H, Hojjatoleslamy M, Mohammadizadeh M Tags: Int J Biol Macromol Source Type: research

Anti-acetylcholinesterase and toxicity against Artemia salina of chitosan microparticles loaded with essential oils of Cymbopogon flexuosus, Pelargonium x ssp and Copaifera officinalis.
The objective of this study was to produce, by spray drying, chitosan microparticles (CMs) loaded with EO of Lemongrass (Cymbopogon flexuosus), Geranium (Pelargonium x ssp) and Copaiba (Copaifera officinalis). Physicochemical and biological characterization of these microparticles showed that CMs presented spherical morphology, had an average size range of 2-3 μm with positive zeta potential (ZP) values, and enhanced thermal stability, compared to free EO. The encapsulation efficiency (EE) ranged from 4.8-58.6%, depending on the oil's properties. In vitro EO release from CMs was determined at different pHs, with 94% r...
Source: International Journal of Biological Macromolecules - November 17, 2020 Category: Biochemistry Authors: Lima LR, Andrade FK, Alves DR, de Morais SM, Vieira RS Tags: Int J Biol Macromol Source Type: research

Impact of heating and drying temperatures on the properties of konjac glucomannan/curdlan blend films.
Abstract The impact of preparation conditions including heating temperature (from 60 °C to 90 °C) and drying temperatures (from 25 °C to 90 °C) on the properties of pure curdlan film and konjac glucomannan (KGM) and curdlan blend films were analyzed. Microstructure analysis indicated the KGM addition could significantly improve the relatively poor film-forming property of curdlan. FTIR and X-ray analysis showed that at high heating temperature 90 °C, molecular interaction might be enhanced in the films due to the stretched structure of curdlan and dissociation of curdlan bundles or tr...
Source: International Journal of Biological Macromolecules - November 17, 2020 Category: Biochemistry Authors: Wu K, Wan Y, Li X, Qian H, Xiao M, Ni X, Jiang F, Chen S Tags: Int J Biol Macromol Source Type: research

The interaction of A β42 peptide in monomer, oligomer or fibril forms with sphingomyelin/cholesterol/ganglioside bilayers.
The interaction of Aβ42 peptide in monomer, oligomer or fibril forms with sphingomyelin/cholesterol/ganglioside bilayers. Int J Biol Macromol. 2020 Nov 17;: Authors: Ahyayauch H, Masserini M, Goñi FM, Alonso A Abstract Aβ42 peptide binds neuronal membranes and aggregates into plaques that are characteristic of Alzheimer's disease. Aβ42 peptide has been proposed to be generated in membrane (nano) domains in the liquid-ordered phase, ganglioside GM1 being a major facilitator of peptide binding to the membrane. The peptide exists in solution in various degrees of aggregation, either...
Source: International Journal of Biological Macromolecules - November 17, 2020 Category: Biochemistry Authors: Ahyayauch H, Masserini M, Goñi FM, Alonso A Tags: Int J Biol Macromol Source Type: research

Immobilization of Aspergillus oryzae β-galactosidase in cation functionalized agarose matrix and its application in the synthesis of lactulose.
Immobilization of Aspergillus oryzae β-galactosidase in cation functionalized agarose matrix and its application in the synthesis of lactulose. Int J Biol Macromol. 2020 Nov 17;: Authors: Serey M, Vera C, Guerrero C, Illanes A Abstract Aspergillus oryzae β-galactosidase was immobilized in in-house quaternary ammonium agarose (QAA) and used for the first time in the synthesis of lactulose. A biocatalyst was obtained with a specific activity of 24,690 IUH∙g-1; protein immobilization yield of 97% and enzyme immobilization yield of 76% were obtained at 30 °C in 10 mM phosphate buffer p...
Source: International Journal of Biological Macromolecules - November 17, 2020 Category: Biochemistry Authors: Serey M, Vera C, Guerrero C, Illanes A Tags: Int J Biol Macromol Source Type: research

Enzymatic glycosylation of bioactive acceptors catalyzed by an immobilized fungal β-xylosidase and its multi-glycoligase variant.
Enzymatic glycosylation of bioactive acceptors catalyzed by an immobilized fungal β-xylosidase and its multi-glycoligase variant. Int J Biol Macromol. 2020 Nov 17;: Authors: Murguiondo C, Mestre A, Méndez-Líter JA, Nieto-Domínguez M, de Eugenio LI, Molina-Gutiérrez M, Martínez MJ, Prieto A Abstract A recombinant β-xylosidase (rBxTW1) from the ascomycete Talaromyces amestolkiae and a mutant derived from it, with mostly synthetic activity, have been immobilized as magnetic cross-linked enzyme aggregates (mCLEAs). The mCLEAs of rBxTW1 kept the excellent hydrol...
Source: International Journal of Biological Macromolecules - November 17, 2020 Category: Biochemistry Authors: Murguiondo C, Mestre A, Méndez-Líter JA, Nieto-Domínguez M, de Eugenio LI, Molina-Gutiérrez M, Martínez MJ, Prieto A Tags: Int J Biol Macromol Source Type: research

Lactoferrin coated or conjugated nanomaterials as an active targeting approach in nanomedicine.
Abstract A successful drug delivery to a specific site relies on two essential factors including; efficient entrapment of the drug within the carrier and successful delivery of drug- loaded nanocarrier to the target site without opsonisation or drug release in the circulation before reaching the organ of interest. Lactoferrin (LF) is a glycoprotein belonging to the transferrin (TF) family which can bind to TF receptors (TFRs) and LF membrane internalization receptors (LFRs) highly expressed on the cell surface of both highly proliferating cancer cells and blood brain barrier (BBB), which in turn can facilitate its...
Source: International Journal of Biological Macromolecules - November 17, 2020 Category: Biochemistry Authors: Agwa MM, Sabra S Tags: Int J Biol Macromol Source Type: research

Reinforcement of hydrogel scaffold using oxidized-guar gum incorporated with curcumin-loaded zein nanoparticles to improve biological performance.
Abstract Newly, the use of biocompatible injectable hydrogel with appropriate features for application in the tissue engineering area as a perfect wound dressing has been more attracted. For this purpose, the curcumin loaded Zein nanoparticles/aldehyde-modified guar gum/silk fibroin (Cur-NPs/OGG/SF) hydrogel networks were successfully developed to increase the Cur bioavailability during the wound treatment procedure. Fabricated hydrogels were assessed for their morphological, thermal stability, degradation, and mechanical features. By varying the OGG/SF weight ratios, the physicochemical features of hydrogels with...
Source: International Journal of Biological Macromolecules - November 16, 2020 Category: Biochemistry Authors: Nezhad-Mokhtari P, Ghorbani M, Yazdani N Tags: Int J Biol Macromol Source Type: research

Classifying the superfamily of small heat shock proteins by using g-gap dipeptide compositions.
Abstract Small heat shock protein (sHSP) is a superfamily of molecular chaperone and is found from archaea to human. Recent researches have demonstrated that sHSPs participate in a series of biological processes and are even closely associated with serious diseases. Since sHSP is a very large superfamily and members from different superfamilies exhibit distinct functions, accurate classification of the subfamily of sHSP will be helpful for unrevealing its functions. In the present work, a support vector machine-based method was proposed to classify the subfamily of sHSPs. In the 10-fold cross validation test, an o...
Source: International Journal of Biological Macromolecules - November 16, 2020 Category: Biochemistry Authors: Feng P, Liu W, Huang C, Tang Z Tags: Int J Biol Macromol Source Type: research

Development of delivery system from marine chitosan: Encapsulation and release kinetic study of antioxidant peptides from chitosan microparticle.
ri M Abstract The present work aims to encapsulate goby fish protein hydrolysate (GPH), endowed with antioxidant activity, through ionic gelation process using blue crab chitosan (CH) and tripolyphosphate anions and to evaluate the structural, thermal and antioxidant properties of the elaborated microparticles (MPs). The GPH-loaded MPs present spherical shape as seen by scanning electron microscopy (SEM) images and positive zeta potential. The increase of loaded GPH concentration led to the increase of encapsulation efficiency (EE) and to the reduction of the particle size. In fact, MPs, loaded with 2 and 5 mg/m...
Source: International Journal of Biological Macromolecules - November 16, 2020 Category: Biochemistry Authors: Nasri R, Hamdi M, Touir S, Li S, Karra-Chaâbouni M, Nasri M Tags: Int J Biol Macromol Source Type: research

Chitosan capping of CuO nanoparticles: Facile chemical preparation, biological analysis, and applications in dentistry.
Abstract This investigation is vital contribution to the healthcare system utilizing techniques of nanobiotechnology. It interestingly applies chitosan capped CuO nanoparticles in the field of medicine and restorative dentistry. The CuO nanoparticles and CuO-Chitosan nanoparticles are prepared by co-precipitation, and their characterization is performed using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy dispersive X-ray (EDX). The average crystallite size of these nanoparticles has been found to be in the dimensions of
Source: International Journal of Biological Macromolecules - November 16, 2020 Category: Biochemistry Authors: Javed R, Rais F, Kaleem M, Jamil B, Ahmad MA, Yu T, Qureshi SW, Ao Q Tags: Int J Biol Macromol Source Type: research

Biomimetic poly( γ-glutamic acid) hydrogels based on iron (III) ligand coordination for cartilage tissue engineering.
Biomimetic poly(γ-glutamic acid) hydrogels based on iron (III) ligand coordination for cartilage tissue engineering. Int J Biol Macromol. 2020 Nov 16;: Authors: Wang P, Zhang W, Yang R, Liu S, Ren Y, Liu X, Tan X, Chi B Abstract For the problems in the research on differentiation of mesenchymal stem cells (BMSCs), such as poor differentiation tendency and low differentiation efficiency, a novel photo-crosslinked extracellular matrix (ECM) inspired double network hydrogel that composed of poly(γ-glutamic acid) (γ-PGA) hydrogel and Fe3+ ligand coordination was designed and manufactured...
Source: International Journal of Biological Macromolecules - November 16, 2020 Category: Biochemistry Authors: Wang P, Zhang W, Yang R, Liu S, Ren Y, Liu X, Tan X, Chi B Tags: Int J Biol Macromol Source Type: research

Self-crosslinked chitosan/dialdehyde xanthan gum blended hypromellose hydrogel for the controlled delivery of ampicillin, minocycline and rifampicin.
; Z, Saha P Abstract The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences. In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin. The prepared hydrogel scaffolds were characterized by rheology method, FTIR, SEM, TGA and compression analysis. In addition, gelation kinetics, swelling, in vitro degradation an...
Source: International Journal of Biological Macromolecules - November 16, 2020 Category: Biochemistry Authors: Ngwabebhoh FA, Zandraa O, Patwa R, Saha N, Capáková Z, Saha P Tags: Int J Biol Macromol Source Type: research