High throughput virtual screening reveals SARS-CoV-2 multi-target binding natural compounds to lead instant therapy for COVID-19 treatment.
Abstract The present-day world is severely suffering from the recently emerged SARS-CoV-2. The lack of prescribed drugs for the deadly virus has stressed the likely need to identify novel inhibitors to alleviate and stop the pandemic. In the present high throughput virtual screening study, we used in silico techniques like receptor-ligand docking, Molecular dynamic (MD), and ADME properties to screen natural compounds. It has been documented that many natural compounds display antiviral activities, including anti-SARS-CoV effect. The present study deals with compounds of Natural Product Activity and Species Source...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Naik B, Gupta N, Ojha R, Singh S, Prajapati VK, Prusty D Tags: Int J Biol Macromol Source Type: research

Batch preparation of electrospun polycaprolactone/chitosan/aloe vera blended nanofiber membranes for novel wound dressing.
In this study, a slopeing free surface electrospinning (SFSE) device was presented to prepare large quantities of polycaprolactone/chitosan/aloe vera (PCL/CS/AV) nanofiber membranes (NFMs) for antibacterial wound dressing. And the morphologies of PCL/CS/AV NFMs with varying weight ratios of PCL:CS:AV were studied using SEM, and the optimal weight ratio of 5:3:2 was determined for better wound dressings. Then the structure, wetting property and yield of the PCL/CS/AV NFMs with the optimal weight ratio were investigated, and the effects of the addition of AV on the antibacterial performance and the biocompatibility of NFMs w...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Yin J, Xu L Tags: Int J Biol Macromol Source Type: research

Bio-based Fe3O4/chitosan nanocomposite sensor for response surface methodology and sensitive determination of gallic acid.
In this study, preparation of a novel bio-sensor based on Fe3O4/chitosan nanocomposite is reported for electrochemical studies and determination of gallic acid (GA). Combination of chitosan with Fe3O4 nanoparticles causes to improve oxidation current of the GA. Characterization of the nanocomposite is carried out by different techniques such as X-ray diffraction, transmission electron microscopy, scanning electron microscopy, vibrating sample magnetometer, electrochemical impedance spectroscopy and cyclic voltammetry. Furthermore, multivariate optimization strategy is applied for simultaneous optimization of the chemical a...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Nazari F, Ghoreishi SM, Khoobi A Tags: Int J Biol Macromol Source Type: research

Naja kaouthia venom protein, Nk-CRISP, upregulates inflammatory gene expression in human macrophages.
Abstract Cysteine-Rich Secretory Proteins (CRISP) are widespread in snake venoms and known to target ion channels. More recently, CRISPs have been shown to mediate inflammatory responses. Involvement of potential receptor in CRISP-induced inflammatory reactions, however, remains unknown. A CRISP protein named as Nk-CRISP, was isolated from the venom of Naja kaouthia. The molecular mass of the purified protein was found to be ~25 kDa and the primary sequence was determined by MALDI TOF-TOF. The involvement of this protein in proinflammatory effects were evaluated in THP-1 macrophage-like cells. Nk-CRISP treated c...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Deka A, Sharma M, Mukhopadhyay R, Devi A, Doley R Tags: Int J Biol Macromol Source Type: research

In situ mineralization of nano-hydroxyapatite on bifunctional cellulose nanofiber/polyvinyl alcohol/sodium alginate hydrogel using 3D printing.
This study shows that 3D printed sodium alginate/PVA-grafted CNF hydrogels are promising scaffold materials for bone tissue engineering. PMID: 32470581 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Abouzeid RE, Khiari R, Salama A, Diab M, Beneventi D, Dufresne A Tags: Int J Biol Macromol Source Type: research

A facile strategy towards heterogeneous preparation of thermoplastic cellulose grafted polyurethane from amorphous regenerated cellulose paste.
Abstract Cellulose is an abundant feedstock with renewability and biodegradability. However, it is still challenging to manufacture natural cellulose products by environmentally friendly thermoplastic processing methods. Herein, we proposed a green approach for the heterogeneous preparation of thermoplastic cellulose grafted polyurethane (RCP-g-PU) from amorphous regenerated cellulose paste (RCP) via hydroxyl/isocyanate chemistry. First, amorphous RCP was fabricated through dissolving cellulose in sodium hydroxide aqueous solution and regenerating in polyethylene glycol, resulting in the enhancement of the accessi...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Hou DF, Liu ZY, Zhou L, Tan H, Yang W, Yang MB Tags: Int J Biol Macromol Source Type: research

Production of neoagarobiose from agar through a dual-enzyme and two-stage hydrolysis strategy.
In this study, two novel β-agarases (AgaA and AgaB) were screened and characterized. It was found that the AgaA was an endo-type agarase which could efficiently hydrolyzed agar or agarose to form neoagarobiose (NA2), neoagarotetraose (NA4) and neoagarohexaose (NA6), while the AgaB was an exo-type and bifunctional enzyme that showed activities towards both agarose and porphyran. Based on the properties of the two enzymes, we developed modular strategy for enzymatic production of neoagarobiose through a two-stage hydrolysis reaction. The cheap substrate agar was first liquefied by AgaA at high temperature to form neoaga...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Yan J, Chen P, Zeng Y, Yang J, Men Y, Zhu Y, Sun Y Tags: Int J Biol Macromol Source Type: research

Modeling of the release kinetics of phenolic acids embedded in gelatin/chitosan bioactive-packaging films: Influence of both water activity and viscosity of the food simulant on the film structure and antioxidant activity.
t F Abstract Hydrocolloid-based films containing natural phenolic antioxidants (gallic and trans-cinnamic acids at 5% w/wt of polymers) embedded in a gelatin/chitosan matrix were designed as sustainable active packaging. This work deals with characterizing the release mechanisms of the phenolic acids from the films immersed into food simulants (sugar or polyol solutions) having different water activities and viscosities. The films containing gallic acid exhibited higher antioxidant activities than the trans-cinnamic acid films. The use of sucrose or glycerol to reduce the aw both decreases the iron chelating power...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Benbettaïeb N, Mahfoudh R, Moundanga S, Brachais CH, Chambin O, Debeaufort F Tags: Int J Biol Macromol Source Type: research

New formulations based on salicyl-imine-chitosan hydrogels for prolonged drug release.
The objective of this paper was to investigate the new formulations based on salicyl-imine-chitosan hydrogels as potential controlled drug release systems. They were prepared by in situ hydrogelation of chitosan with salicylaldehyde in the presence of diclofenac sodium salt (DCF) as model drug. FTIR, X-ray Spectroscopy, POM and SEM techniques were used to confirm the structural, supramolecular and morphological particularities of the formulations. Swelling test, in vitro enzymatic biodegradation and release profile were investigated in similar conditions mimicking the in vivo environment, and the release mechanism was asse...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Iftime MM, Tartau LM, Marin L Tags: Int J Biol Macromol Source Type: research

Antibacterial, anticoagulant and cytotoxic evaluation of biocompatible nanocomposite of chitosan loaded green synthesized bioinspired silver nanoparticles.
Abstract Present work reports the green synthesis of chitosan functionalized silver nanoparticles (CS-AgNPs) using ethanolic buds extract of Sygyzium aromaticum. CS-AgNPs were characterized physically, evaluated for antibacterial, anticoagulant and antiplatelet activities, and toxicity profile. The physical characterization of CS-AgNPs was done by UV/vis, SEM, TEM, FTIR and EDX. The sphericity was found uniform. FTIR and EXD showed noninterfering few impurities. The antibacterial activity against VRSA (ZI, 23.2 ± 0.51 mm) and MRSA (ZI, 25.8 ± 0.32 mm) were determined. The rise in bleeding...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Asghar MA, Yousuf RI, Shoaib MH, Asghar MA Tags: Int J Biol Macromol Source Type: research

Preparation and characterization of grass carp collagen-chitosan-lemon essential oil composite films for application as food packaging.
Abstract Encapsulation effectively delays the volatilization of lemon essential oil (LEO). Here, various chitosan (CS)-LEO nanoparticles were prepared by emulsification using different CS and Tween-80 concentrations and CS:TPP and CS:LEO ratios. The CS-LEO nanoparticles were spherical, small in size (58 ± 9 nm), had a low polydispersity index (0.15), and were highly stable under the right conditions. FTIR spectra indicated that they were fully encapsulated in the films. The composite films are characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron mic...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Jiang Y, Lan W, Sameen DE, Ahmed S, Qin W, Zhang Q, Chen H, Dai J, He L, Liu Y Tags: Int J Biol Macromol Source Type: research

Agar/ κ-carrageenan composite hydrogel adsorbent for the removal of Methylene Blue from water.
Agar/κ-carrageenan composite hydrogel adsorbent for the removal of Methylene Blue from water. Int J Biol Macromol. 2020 May 26;: Authors: Duman O, Polat TG, Diker CÖ, Tunç S Abstract Water pollution caused by dyes is a significant global problem. Adsorption technique is successfully applied for the removal of dyes from water. In this technique, the use of effective adsorbents is very important. Here, agar/κ-carrageenan composite hydrogel adsorbent prepared through free radical cross-linking reaction in the presence of tri (ethylene glycol) divinyl ether as a cross-linker and ch...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Duman O, Polat TG, Diker CÖ, Tunç S Tags: Int J Biol Macromol Source Type: research

Antibacterial nanobiocomposite based on halloysite nanotubes and extracted xylan from bagasse pith.
In this study, the antibacterial polyelectrolyte carboxymethyl xylan/chitosan (CMX/CS) films incorporated with halloysite nanotubes (HNTs) and Origanum vulgare essential oil (OEO) were prepared and then characterized. Xylan-rich hemicelluloses (62.23%) were extracted from sugarcane bagasse pith and then subjected to carboxymethylation. FTIR analysis revealed the successful modification of hemicelluloses. The irregular white spots in SEM images of nanobiocomposites revealed the entrance of HNTs into the polymer matrix. EDX maps manifested that the density of Si and Al atoms increased, as the amount of HNT increased. The obs...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Yousefi P, Hamedi S, Garmaroody ER, Koosha M Tags: Int J Biol Macromol Source Type: research

The effects of crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different crosslinking manners were designed and fabricated, and the effects of crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated. The gelation time, mechanical property, swelling and degradability of hydrogels were characterized. Chondrocytes were encapsulated into these hydrogels to detect their effects on cell survival, proliferation, morphology and ECM secretion. Furthermore, the hydrogels were implanted into the back of SD rats to evaluate their biodegradability...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Kong W, Gao Y, Liu Q, Dong L, Guo L, Fan H, Fan Y, Zhang X Tags: Int J Biol Macromol Source Type: research

Utilization of cinnamaldehyde and zinc oxide nanoparticles in a carboxymethylcellulose-based composite coating to improve the postharvest quality of cherry tomatoes.
Abstract Carboxymethylcellulose (CMC)-based film packaging enriched with plant-sourced cinnamaldehyde (CIN) and zinc oxide nanoparticles (ZnONPs) of simple synthesis were prepared in this work. Then, the physico-mechanical and barrier properties and antifungal activities of CMC-based films, including pristine CMC, CIN/CMC, ZnONPs/CMC, ZnONPs/CIN/CMC were investigated. The results indicated that the ZnONPs incorporation produced a nanocomposite film with low transparency, good flexibility and high mechanical resistance. Furthermore, the water barrier capacity and antifungal performance of CMC-based films were signi...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Guo X, Chen B, Wu X, Li J, Sun Q Tags: Int J Biol Macromol Source Type: research

Immobilized TiO2/chitosan beads for photocatalytic degradation of 2,4-dichlorophenoxyacetic acid.
Abstract Advancement in photocatalysis focuses on large-scale commercialization where the immobilization techniques gain attention with an aim to recover and reuse the catalyst for the redemption of pollutants. TiO2 will act as a potential catalyst and chitosan, a natural biopolymer is used to immobilize TiO2. 2,4-Dicholorophenoxyacetic acid, a common broadleaf pesticide found in surface and groundwater is taken as a model pollutant. Thus, the objective is to study TiO2/chitosan beads for the degradation of 2,4-dicholorophenoxyacetic acid. TiO2/chitosan beads were prepared by the phase inversion method and studied...
Source: International Journal of Biological Macromolecules - May 26, 2020 Category: Biochemistry Authors: Balakrishnan A, Sowmya A, Gopalram K Tags: Int J Biol Macromol Source Type: research

Interaction of LysM BON family protein domain with carbapenems: A putative mechanism of carbapenem resistance.
This study further revealed a strong binding of both antibiotics to LysM domain protein. Molecular simulation studies of LysM domain protein with meropenem and imipenem for 80 ns has also showed stable structure. We concluded that overexpressed LysM domain under induced condition interacted with carbapenems, leading to enhanced resistance as proved by high MIC values. Hence, the study proved the proposed hypothesis that the LysM domain plays a significant role in the putative mechanism of antibiotics resistance. PMID: 32464197 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Ali A, Kumar R, Khan A, Khan AU Tags: Int J Biol Macromol Source Type: research

Efficient expression of novel glutamate decarboxylases and high level production of γ-aminobutyric acid catalyzed by engineered Escherichia coli.
Efficient expression of novel glutamate decarboxylases and high level production of γ-aminobutyric acid catalyzed by engineered Escherichia coli. Int J Biol Macromol. 2020 May 25;: Authors: Tang CD, Li X, Shi HL, Jia YY, Dong ZX, Jiao ZJ, Wang LF, Xu JH, Yao LG, Kan YC Abstract Glutamate decarboxylase (GAD) has the potential of converting L-glutamate to gamma-aminobutyric acid (GABA), which is an important non-proteinogenic amino acid that has a potential use as food additive or dietary supplement for its physiological functions. A novel pyridoxal 5'-phosphate (PLP)-dependent glutamate decarboxy...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Tang CD, Li X, Shi HL, Jia YY, Dong ZX, Jiao ZJ, Wang LF, Xu JH, Yao LG, Kan YC Tags: Int J Biol Macromol Source Type: research

Purification and biochemical characterization of Arabian balsam α-amylase and enhancing the retention and reusability via encapsulation onto calcium alginate/Fe2O3 nanocomposite beads.
In this study, Arabian balsam α-amylase was purified using the three-step purification method with 9.8-fold purification and 7% recovery. The purified α-amylase's molecular weight was 85 kDa. Calcium alginate incorporated with iron (III) oxide nanoparticles was applied as an immobilizing support for α-amylase. The immobilized α-amylase was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy. In addition, the optimum conditions for immobilization efficiency, immobilization time, reusability, kinetic parameters, and the effect of pH ...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Al-Harbi SA, Almulaiky YQ Tags: Int J Biol Macromol Source Type: research

Inhibitory effects of four anthraquinones on tyrosinase activity: Insight from spectroscopic analysis and molecular docking.
In this study, the inhibitory effects of four anthraquinones including chrysophanol, emodin, physcione and rhein on tyrosinase were investigated by enzyme inhibition assay. The results indicated that all of anthraquinones could significantly inhibit the activity of tyrosinase in a competitive manner. To gain insight into the inhibitory mechanism of anthraquinones on tyrosinase, spectroscopic analysis combined with molecular docking studies were performed. Fluorescence results showed that anthraquinones interacted with tyrosinase by static quenching in a molecular ratio of 1:1. Circular dichroism and molecular docking sugge...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Zeng HJ, Sun DQ, Chu SH, Zhang JJ, Hu GZ, Yang R Tags: Int J Biol Macromol Source Type: research

Immunoprotection against lethal effects of Crotalus durissus snake venom elicited by synthetic epitopes trapped in liposomes.
Olortegui C Abstract Snakebites caused by Crotalus genus are the second most frequent in Brazil. Crotoxin is a beta-neurotoxin responsible for the main envenomation effects of Crotalus biting, while crotamine immobilizes the animal hind limbs, contributing to prey immobilization and to envenoming symptoms. As crotoxin and crotamine represent about 90% of Crotalus venom dry weight, these toxins are of great importance for antivenom therapy. In this sense, knowledge regarding the antigenicity/immunogenicity at the molecular level of these toxins can provide valuable information for the improvement of specific antive...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Vaz de Melo PD, de Almeida Lima S, Araújo P, Santos RM, Gonzalez E, Belo AA, Machado-de-Ávila RA, Costal-Oliveira F, Soccol VT, Guerra-Duarte C, Rezende L, Chavez-Olortegui C Tags: Int J Biol Macromol Source Type: research

Interactions of fibroblast growth factors with sulfated galactofucan from Saccharina japonica.
Abstract A total 68 types of marine algae oligosaccharides and polysaccharides were prepared and used to study the structure-activity relationship of oligosaccharides and polysaccharides in their interactions with fibroblast growth factors (FGF) 1 and 2. Factors considered include different types of algae, extraction methods, molecular weight, sulfate content and fractions. In the case of low molecular weight polysaccharide (SJ-D) from Saccharina japonica and its fractions eluting from anion exchange column, both 1.0 M NaCl fraction (SJ-D-I) and 2.0 M NaCl fraction (SJ-D-S) had stronger binding affinity than t...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Jin W, Jiang D, Zhang W, Wang C, Xia K, Zhang F, Linhardt RJ Tags: Int J Biol Macromol Source Type: research

Green synthesized and characterized copper nanoparticles using various new plants extracts aggravate microbial cell membrane damage after interaction with lipopolysaccharide.
In conclusion, these obtained CuNPs could be precisely applied in treatment protocols without any covering or core-shell procedures. PMID: 32464203 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Asghar MA, Asghar MA Tags: Int J Biol Macromol Source Type: research

Preparation and characterization of crosslinked porous starch hemostatic.
Abstract In order to develop starch hemostatic materials with excellent hemostatic properties, the preparation of crosslinked porous starch (SPS) with sodium trimetaphosphate (STMP) as cross-linking agent was studied in this paper. When the solid-liquid ratio of porous starch (PS) was 30%, the mass ratio of cross-linking agent to starch was 0.04-1, and the SPS was crosslinked at pH 10.0, 55 °C for 50-60 min, the water absorption ratio and swelling ratio of SPS reach up to 160.5% and 239.1%, respectively. The characterization by infrared spectra, scanning electron microscopy and X-Ray diffraction spectra ...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Qian J, Chen Y, Yang H, Zhao C, Zhao X, Guo H Tags: Int J Biol Macromol Source Type: research

Maximizing the direct recovery and stabilization of cellulolytic enzymes from Trichoderma harzanium BPGF1 fermented broth using carboxymethyl inulin nanoparticles.
Abstract The carboxymethylated inulin (CMI) nanoparticles prepared by the salt out method was demonstrated to harvest cellulolytic enzymes (Ez) directly from the clarified fermented broth of Trichoderma harzanium BPGF1. The formation of CMI nanoparticles and entrapment of Ez in CMI was confirmed by scanning electron microscopy and Fourier transform infrared spectroscopy, respectively. A factorial design was developed to maximize enzymes recovery directly from the fermented broth. A maximum of 71.68 ± 8.61% cellulolytic enzymes was recovered using 20 mg/L inulin, 2 M sodium chloroacetate at 80 °...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Vaidyanath S, Harish BS, Gayathri G, Trilokesh C, Uppuluri KB, Anbazhagan V Tags: Int J Biol Macromol Source Type: research

Cyclocarya paliurus polysaccharide alleviates liver inflammation in mice via beneficial regulation of gut microbiota and TLR4/MAPK signaling pathways.
Abstract The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism. Based on a mouse model of CCl4 induced liver inflammation and pretreatment with CP. Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver. TLR4 and MAPK pathways were down-regulated after CP administration. Furthermore, 16S rDNA showed that CP increased the Firmicutes/Bacteroidetes ratio...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Wu T, Shen M, Guo X, Huang L, Yang J, Yu Q, Chen Y, Xie J Tags: Int J Biol Macromol Source Type: research

In-silico and biophysical investigation of biomolecular interaction between naringin and nsP2 of the chikungunya virus.
In conclusion, this report highlights the potential of naringin as an anti-viral agent against Chikungunya. PMID: 32464207 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Tripathi PK, Singh J, Gaurav N, Garg DK, Patel AK Tags: Int J Biol Macromol Source Type: research

Preparation of porous chitosan/carboxylated carbon nanotube composite aerogels for the efficient removal of uranium(VI) from aqueous solution.
Abstract The porous chitosan/carboxylated carbon nanotubes composite aerogels (CS-CCN) with different CCN contents were prepared for the efficient removal of U(VI) from aqueous solution. The successful formation of CS-CCN aerogels with highly porous structure was confirmed by different characterizations (such as SEM, TEM, XRD, etc.). The sorption capacity of the aerogels depends on CCN content, which has significant impact on the porous structure and the sorption ability of the aerogels. The CS-CCN aerogels were found to be very effective for U(VI) sorption: the maximum mono-layer sorption capacity for CS-CCN2 aer...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Tang X, Zhou L, Le Z, Wang Y, Liu Z, Huang G, Adesina AA Tags: Int J Biol Macromol Source Type: research

Predictive and fluorescent nanosensing experimental methods for evaluating anthrax protective antigen and lethal factor interactions for therapeutic applications.
In this study, theoretical and experimental approaches were used to monitor the interactions of PAD4 and LFD1. CLusPro server and Dimplot software were used to predict the interaction of these domains. Results, revealed interactive sites between PAD4 and LFD1 on loop regions of both C and N terminal of PAD4. In experimental methods, PAD4 and LFD1 were expressed in Escherichia coli and purified for usage in Magnetic Bead (MB) and Multi-Walled Carbon Nanotubes (MWCNTs) based bio-sensing platforms. In the magnetic-based system, the magnetic sedimentation of QD-PAD4 by MBs-LFD1 and the observation of the fluorescence spectrum ...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Sadeghpour SD, Karimi F, Alizadeh H Tags: Int J Biol Macromol Source Type: research

Oligo(lactic acid)-grafted starch: A compatibilizer for poly(lactic acid)/thermoplastic starch blend.
The objective of this study was to improve the compatibility between poly(lactic acid) (PLA) and thermoplastic starch (TPS) in PLA/TPS blown films by incorporating oligo(lactic acid)-grafted starch (OLA-g-starch) as a compatibilizer. OLA-g-starch with a degree of substitution of 1.2 and degree of polymerization of 2.0 was synthesized via a ring-opening polymerization of lactide initiated by hydroxyl groups of starch. The PLA/TPS blends containing OLA-g-starch were prepared using a twin-screw extruder with a constant weight proportion of PLA to TPS of 50:50 and various concentrations of OLA-g-starch, i.e., 1, 2, 3, and 5%. ...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Noivoil N, Yoksan R Tags: Int J Biol Macromol Source Type: research

Formulation of active food packaging by design: Linking composition of the film-forming solution to properties of the chitosan-based film by response surface methodology (RSM) modelling.
vak U Abstract An active chitosan-based film, blended with the hydrolysable tannin-rich extract obtained from fibrous chestnut wood (Castanea sativa Mill.), underwent a simultaneous engineering optimization in terms of measured moisture content (MC), tensile strength (TS), elongation at break (EB), and total phenolic content (TPC). The optimal product formulation for a homogeneous film-forming solution was sought by designing an empirical Box-Behnken model simulation, based on three independent variables: the concentrations of chitosan (1.5-2.0% (w/v)), extracted powder-form chestnut extract (0.5-1.0% (w/v)) and p...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Bajić M, Oberlintner A, Kõrge K, Likozar B, Novak U Tags: Int J Biol Macromol Source Type: research

A systematic study on chitosan-liposome based systems for biomedical applications.
Abstract The advent of liposome technology with its unique features has led researchers to work relentlessly on the successful development of novel drug delivery vehicles based on liposomes. But still there are some limitations of using liposomes for biomedical applications because of their poor stability that is primarily the cause of rapid leakage of drugs incorporated within the said matrices. Therefore, a considerable interest has been paid on modification of surface of liposomes by combining it with several compounds of interest. Although chitosan-liposome based systems are not yet well-documented. Hence, in ...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Kumar S, Dutta J, Dutta PK, Koh J Tags: Int J Biol Macromol Source Type: research

Delivery of polysaccharides from Ophiopogon japonicus (OJPs) using OJPs/chitosan/whey protein co-assembled nanoparticles to treat defective intestinal epithelial tight junction barrier.
In this study, OJPs extracted and fractionated from Ophiopogon japonicus were used to prepare OJPs/chitosan (CS)/whey protein (WP) co-assembled nanoparticles. The OJPs/CS/WP nanoparticles showed high biocompatibility and inhibited the cytotoxicity of RAW264.7 cells induced by nickel. With the assistance of CS and WP, the anti-inflammatory and antioxidant activities of OJPs were enhanced because the nanoparticles improved OJPs uptake by RAW264.7 macrophage cells as evidenced by efficient scavenging of DPPH and ABTS free radicals and effective inhibition of NO production and the gene expressions of iNOS, COX2, TNF-α, C...
Source: International Journal of Biological Macromolecules - May 25, 2020 Category: Biochemistry Authors: Lin C, Kuo TC, Lin JC, Ho YC, Mi FL Tags: Int J Biol Macromol Source Type: research

Simple physical adsorption technique to immobilize Yarrowia lipolytica lipase purified by different methods on magnetic nanoparticles: Adsorption isotherms and thermodynamic approach.
Abstract Magnetic nanoparticles (Fe3O4) were used for physical adsorption of lipase from Yarrowia lipolytica IMUFRJ 50682. The optimal adsorption conditions were obtained as follows: enzyme/support 19.3 mg/g and temperature of 20 °C for standard protein. High immobilization efficiency of 99% was obtained for 4 mL of crude lipase extract (containing 0.315 mg protein/mL) and 0.02 g of magnetic nanoparticles and this biocatalyst was recycled 30 times with 70% of lipase activity in the end. Purified lipase extracts were also efficiently immobilized and ultrafiltered lipase extract (ULE) and aqueous two-p...
Source: International Journal of Biological Macromolecules - May 23, 2020 Category: Biochemistry Authors: Carvalho T, Pereira ADS, Bonomo RCF, Franco M, Finotelli PV, Amaral PFF Tags: Int J Biol Macromol Source Type: research

Comparative analysis of RNA-binding proteomes under Arabidopsis thaliana-Pst DC3000-PAMP interaction by orthogonal organic phase separation.
Abstract RNA-binding proteins (RBPs) are pivotal participants in post-transcriptional gene regulation. They interact with RNA directly to perform several post-transcriptional RNA regulatory functions or direct metabolic processes. Despite the essential importance, the understanding of plant RBPs is elementary, which derives mainly from other kingdoms via bioinformatic extrapolation or mRNA-binding proteins captured through UV crosslinked method. Recently, orthogonal organic phase separation (OOPS) method for RBP identification has been used in mammals and Escherichia coli. And plentiful RBPs were enriched without ...
Source: International Journal of Biological Macromolecules - May 23, 2020 Category: Biochemistry Authors: Liu J, Zhang C, Jia X, Wang W, Yin H Tags: Int J Biol Macromol Source Type: research

Morphological, chemical and thermal analysis of cellulose nanocrystals extracted from bamboo fibre.
In this study, bamboo fibre was subjected to acid hydrolysis for efficient isolation of CNC from bamboo fibre. The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM). The energy Dispersive X-rays (EDX) provided the elemental composition of the prepared CNC's and X-ray diffractometer (XRD) exhibited their crystallinity. The physiochemical analysis was done via Fourier Transform Infrared (FTIR); and their thermal analysis was revealed by Thermogravimetric Analysis (TGA) and Differential scanning calorimetry (DSC)...
Source: International Journal of Biological Macromolecules - May 23, 2020 Category: Biochemistry Authors: Rasheed M, Jawaid M, Parveez B, Zuriyati A, Khan A Tags: Int J Biol Macromol Source Type: research

Transcription-facilitating histone chaperons interact with genomic and synthetic G4 structures.
Abstract Affinity for G-quadruplex (G4) structures may be a common feature of transcription-facilitating histone chaperons (HCs). This assumption is based on previous unmatched studies of HCs FACT, nucleolin (NCL), BRD3, and ATRX. We verified this assumption and considered its implications for the therapeutic applications of synthetic (exogenous) G4s and the biological significance of genomic G4s. First, we questioned whether exogenous G4s that recognize cell-surface NCL and could trap other HCs in the nucleus are usable as anticancer agents. We performed in vitro binding assays and selected leading multi-targeted...
Source: International Journal of Biological Macromolecules - May 23, 2020 Category: Biochemistry Authors: Pavlova II, Tsvetkov VB, Isaakova EA, Severov VV, Khomyakova EA, Lacis IA, Lazarev VN, Lagarkova MA, Pozmogova GE, Varizhuk AM Tags: Int J Biol Macromol Source Type: research

The inefficacy of donepezil on glycated-AChE inhibition: Binding affinity, complex stability and mechanism.
Abstract Donepezil (DPZ) is a well-known drug for Alzheimer's disease that inhibits acetylcholinesterase activity (AChE). In the present study, the inhibitory effect of DPZ on non-enzymatic glycated-AChE (GLY-AChE) was studied by different experimental and simulation techniques. The initial investigation revealed that glycation process could reduce AChE activity approximately 60% in the pure enzyme and 38% in the extracted crude AChE from neural cells cultured in the presence of high glucose (HG) concentration. It is suggested that glycation of lysine residues on the structure of AChE could change the conformation...
Source: International Journal of Biological Macromolecules - May 23, 2020 Category: Biochemistry Authors: Yekta R, Sadeghi L, Dehghan G Tags: Int J Biol Macromol Source Type: research

A novel acid phosphatase from cactus (Opuntia megacantha Salm-Dyck) cladodes: Purification and biochemical characterization of the enzyme.
avi A Abstract Acid phosphatase (ACP) plays an important role in regulating phosphate nutrition in plants. Herein, for the first time, a novel ACP from Opuntia megacantha Salm-Dyck cladodes was purified to homogeneity and biochemically characterized. Specific activity of 8.78 U/mg was obtained with 11.29-fold purification and 15% yield. ACP was purified as monomer with molecular weight of 44 kDa as determined by SDS-PAGE under denaturing and nondenaturing conditions. Optimum pH and temperature for ACP activity was 5.5 and 60 °C, respectively. Thermodynamic parameters (Ea, ΔH, ΔG and ΔS)...
Source: International Journal of Biological Macromolecules - May 23, 2020 Category: Biochemistry Authors: Chafik A, Essamadi A, Çelik SY, Mavi A Tags: Int J Biol Macromol Source Type: research

Chemical structure, anti-inflammatory and antinociceptive activities of a sulfated polysaccharide from Gracilaria intermedia algae.
Freitas ALP Abstract The present work aimed at carrying out the isolation and biochemical characterization of a sulfated polysaccharide fraction (PLS) from the marine algae Gracilaria intermedia and investigating its anti-inflammatory and antinociceptive potential. PLS was obtained through enzymatic digestion with papain and analyzed by means of gel permeation chromatography and Nuclear Magnetic Resonance to 1H and 13C. In order to evaluate the potential of anti-inflammatory action of PLS, we performed paw edema induced by carrageenan, dextran, compound 48/80, histamine and serotonin. In addition, we also measured...
Source: International Journal of Biological Macromolecules - May 22, 2020 Category: Biochemistry Authors: Costa LEC, Brito TV, Silva RO, Sousa WM, Barros FCN, Sombra VG, Júnior JSC, Magalhães DA, Souza MHLP, Medeiros JR, de Paula RCM, Barbosa ALR, Freitas ALP Tags: Int J Biol Macromol Source Type: research

Biological evaluation of BF2-naphthyridine compounds: Tyrosinase and acetylcholinesterase activity, CT-DNA and HSA binding property evaluations.
Abstract The present work reports the biological assays between synthetic BF2-naphtyridine complexes and four proteins: human serum albumin (HSA), calf-thymus DNA (CT-DNA), tyrosinase and acetylcholinesterase enzymes via spectroscopic analysis at physiological conditions, combined with molecular docking simulations. The BF2-complexes presented spontaneous and moderate binding ability to HSA through the ground-state association (static fluorescence quenching mechanism). The main binding site is Sudlow's site I (subdomain IIA) and the binding does not perturb significantly both secondary and surface structure of HSA...
Source: International Journal of Biological Macromolecules - May 22, 2020 Category: Biochemistry Authors: Chaves OA, Calheiro TP, Netto-Ferreira JC, de Oliveira MCC, Franceschini SZ, de Salles CMC, Zanatta N, Frizzo CP, Iglesias BA, Bonacorso HG Tags: Int J Biol Macromol Source Type: research

A combined-modification method of carboxymethyl β-cyclodextrin and lignin for nano-hydroxyapatite to reinforce poly(lactide-co-glycolide) for bone materials.
This study may offer an important guidance to obtain PLGA-based composites reinforced by surface-modified n-HA as bone materials. PMID: 32450324 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 22, 2020 Category: Biochemistry Authors: Chunyan T, Haojie D, Shuo T, Liuyun J, Bingli M, Yue W, Na Z, Liping S, Shengpei S Tags: Int J Biol Macromol Source Type: research

An injectable bioactive magnesium phosphate cement incorporating carboxymethyl chitosan for bone regeneration.
Abstract Magnesium phosphate cement (MPC) can be injected to form an in situ scaffold to repair bone defects. Here we synthesized novel injectable bioactive cements (CMPCs) by incorporating different ratios of carboxymethyl chitosan (CMC, 0-10%) into MPC. The physiochemical properties, compositions, and microstructures of CMPCs were evaluated. The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation were quantified and the underlying cellular mechanisms investigated. CMPCs had longer setting times and lower setting temperatures. CMPC injectabi...
Source: International Journal of Biological Macromolecules - May 22, 2020 Category: Biochemistry Authors: Yu L, Xia K, Gong C, Chen J, Li W, Zhao Y, Guo W, Dai H Tags: Int J Biol Macromol Source Type: research

Xyloglucan as green renewable biopolymer used in drug delivery and tissue engineering.
Abstract Xyloglucan is a mucoadhesive polysaccharide which is extracted from the cell wall of vascular plants. Tamarind seeds are the abundant source of xyloglucan and commercially more popular. It is biocompatible, biodegradable, and nontoxic in nature. It is approved by the FDA for use as a food additive, stabilizing and thickening agent or gelling agent. Recently, many researchers are giving attention to xyloglucan for drug delivery through various routes and regeneration of the number of tissues. However, the findings of tissue regeneration and drug delivery in combination are not collected and represented in ...
Source: International Journal of Biological Macromolecules - May 22, 2020 Category: Biochemistry Authors: Dutta P, Giri S, Giri TK Tags: Int J Biol Macromol Source Type: research

Pre-protective effect of polysaccharides purified from Hericium erinaceus against ethanol-induced gastric mucosal injury in rats.
Abstract The β-glucan H6PC20 (Mw: 2390 kDa) and α-heteropolysaccharide HPB-3 (Mw: 15 kDa) were purified from the fruiting body of Hericium erinaceus according to the previous methods. Their gastroprotective activities and corresponding structure-activity relationship were studied in the ethanol-induced gastric ulcer model of rats. After intragastric administrated with H6PC20 and HPB-3 for 14 days, macroscopic and histological evaluation of gastric mucosa was improved significantly. The defense and repair factors (EGF, bFGF and PGE2) were increased, meanwhile, the inflammatory cytokines (IL-1β...
Source: International Journal of Biological Macromolecules - May 22, 2020 Category: Biochemistry Authors: Chen W, Wu D, Jin Y, Li Q, Liu Y, Qiao X, Zhang J, Dong G, Li Z, Li T, Yang Y Tags: Int J Biol Macromol Source Type: research

New strategy for grafting hydrophobization of lignocellulosic fiber materials with octadecylamine using a laccase/TEMPO system.
In this study, a novel strategy for hydrophobization of lignocelluloses was investigated, which involved the laccase/TEMPO-mediated grafting of octadecylamine (OA) onto both lignin and cellulose components of jute fabrics. The results showed that OA monomers were successfully grafted onto jute fabric surface using the laccase/TEMPO system with the grafting percentage and efficiency values of 0.712% and 10.571%, respectively. The primary hydroxyl groups of cellulose were oxidized by laccase/TEMPO to carbonyl groups, which were then coupled with amino-contained OA monomers via Schiff base reaction. The phenolic hydroxyl grou...
Source: International Journal of Biological Macromolecules - May 22, 2020 Category: Biochemistry Authors: Dong A, Teklu KM, Wang W, Fan X, Wang Q, Ardanuy M, Dong Z Tags: Int J Biol Macromol Source Type: research

Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.
Abstract Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect. Until now, it's still a challenge to control the leakage of MMT and avoid its side-effects. Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control. Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activati...
Source: International Journal of Biological Macromolecules - May 21, 2020 Category: Biochemistry Authors: Liu C, Liu C, Yu S, Wang N, Yao W, Liu X, Sun G, Song Q, Qiao W Tags: Int J Biol Macromol Source Type: research

Dendritic cell-mediated cancer immunotherapy with Ecklonia cava fucoidan.
Abstract Fucoidan is known to exert immunomodulatory effects in animals and humans. Here, we extracted fucoidan from Ecklonia cava (ECF) and evaluated its immunostimulatory and anticancer activities in mice. Treatment with ECF resulted in the activation of bone marrow-derived dendritic cells (BMDCs) in vitro and splenic DCs in vivo. Moreover, the combination of ECF and ovalbumin (OVA) promoted OVA-specific T cell proliferation and cytokine production, which consequently suppressed B16-OVA tumor growth in vivo. The combination treatment with ECF and carcinoma self-antigen resulted in the inhibition of the growth of...
Source: International Journal of Biological Macromolecules - May 21, 2020 Category: Biochemistry Authors: Park HB, Hwang J, Lim SM, Zhang W, Jin JO Tags: Int J Biol Macromol Source Type: research

Extraction optimization, characterization and the antioxidant activities in vitro and in vivo of polysaccharide from Pleurotus ferulae.
PMID: 32446895 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 21, 2020 Category: Biochemistry Authors: Wang W, Li X, Chen K, Yang H, Jialengbieke B, Hu X Tags: Int J Biol Macromol Source Type: research

Fucoidan from acid-processed Hizikia fusiforme attenuates oxidative damage and regulate apoptosis.
In this study, the antioxidant effect of JHCF4 was evaluated. Among the fractions, JHCF4 showed the highest scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), alkyl, and hydroxyl radicals, as well as protective effect against reactive oxygen species (ROS) in 2, 2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-treated Vero cells. Furthermore, JHCF4 showed a protective activity against AAPH-induced apoptosis, as observed by nuclear staining with Hoechst 33342. Our results showed that JHCF4 can up-regulate Bcl-xL, down-regulate Bax and cleave caspase-3 with increased concentrations in AAPH-induced Vero cell...
Source: International Journal of Biological Macromolecules - May 21, 2020 Category: Biochemistry Authors: Dai YL, Jiang YF, Lu YA, Kang MC, Jeon YJ Tags: Int J Biol Macromol Source Type: research