Catalytic properties of a short manganese peroxidase from Irpex lacteus F17 and the role of Glu166 in the Mn2+-independent activity.
Abstract Il-MnP1 (GenBank: AGO86670.2) has been confirmed by sequence analysis as a short manganese peroxidase (MnP) from Irpex lacteus F17 (CCTCC AF 2014020). To investigate the catalytic properties, the oxidation of typical aromatic substrates and the pathways of guaiacol oxidation by Il-MnP1, both in the presence and absence of Mn2+ at either pH 4.0 or pH 7.4, were analyzed. Results showed that Il-MnP1 exhibited higher oxidative activity in the presence of Mn2+ than in the absence of Mn2+ toward the majority of the selected substrates at pH 4.0. Additionally, the similar product compositions suggested tha...
Source: International Journal of Biological Macromolecules - June 18, 2019 Category: Biochemistry Authors: Li L, Liu B, Yang J, Zhang Q, He C, Jia R Tags: Int J Biol Macromol Source Type: research

Identification of a novel UDP-glycosyltransferase gene from Rhodiola rosea and its expression during biotransformation of upstream precursors in callus culture.
In this study, a full length cDNA encoding a UDPG gene of roseroot was identified, cloned and characterized. Its ORF (1425 bp) was transformed into E. coli, where the expression of the recombinant enzyme was confirmed. To monitor the enzyme activity, 3 precursors (tyramine, 4-hydroxyphenylpyruvate & tyrosol) of salidroside biosynthesis pathway were added to roseroot callus cultures and samples were harvested after 1, 6, 12, 24, 48 & 96 h. Along with the controls (without precursor feeding), each sample was subjected to HPLC and qRT-PCR for phytochemical and relative UDP-glycosyltransferase gene expression analy...
Source: International Journal of Biological Macromolecules - June 18, 2019 Category: Biochemistry Authors: Mirmazloum I, Ladányi M, Beinrohr L, Kiss-Bába E, Kiss A, György Z Tags: Int J Biol Macromol Source Type: research

Polysaccharide extracted from Atractylodes macrocephala Koidz (PAMK) induce apoptosis in transplanted H22 cells in mice.
Abstract Polysaccharide of Atractylodes macrocephala Koidz (PAMK) was extracted by alcohol sedimenting ranging from 60% to 90% and purified by ultrafiltration membrane. The high-performance gel permeation chromatography (HPGPC), Fourier-transform infrared spectroscopy (FT-IR), gas chromatography (GC) and Nuclear magnetic resonance (NMR) revealed that PAMK was a 4.1KDa neutral heteropolysaccharide composed of galactose, arabinose and glucose with α-configuration (molar ratio, 1: 1.5: 5). Results of determination of chemical components suggested that PAMK contained 96.47% of polysaccharide and little protein, ...
Source: International Journal of Biological Macromolecules - June 18, 2019 Category: Biochemistry Authors: Feng YY, Ji HY, Dong XD, Yu J, Liu AJ Tags: Int J Biol Macromol Source Type: research

Chlorin e6 decorated doxorubicin encapsulated chitosan nanoparticles for photo-controlled cancer drug delivery.
Abstract In the study we have reported the physico-chemical, photophysical and morphological properties of chlorin e6 (Ce6) decorated doxorubicin (DOX) encapsulated chitosan (CS)-tripolyphosphate (TPP) nanoparticles which prepared by ionotropic gelation method. The Ce6 physically loaded onto the nanoparticles by self-assembly of CS with TPP-DOX under the aqueous conditions. The results from DLS studies highlights the prepared nanoparticles that possess the size in the range of 80-120 nm on with negatively charged -6 mV. The SEM and AFM images showed 80-120 nm size while the average size of the Ce6 decorated ...
Source: International Journal of Biological Macromolecules - June 18, 2019 Category: Biochemistry Authors: Bhatta A, Krishnamoorthy G, Marimuthu N, Dihingia A, Manna P, Biswal HT, Das M, Krishnamoorthy G Tags: Int J Biol Macromol Source Type: research

Characterisation of chitosan molecular weight distribution by multi-detection asymmetric flow-field flow fractionation (AF4) and SEC.
ea FM Abstract A new method for the molecular weight (MW) determination of chitosans by asymmetric flow field flow fractionation (AF-FFF or AF4) coupled with multi-angle light scattering (MALS) and differential refractive index (RI) detectors is proposed. The method allows the separation of polymer from molecular aggregates typically found in chitosan solutions making possible the accurate determination of MW distribution of the polymer. The effect of different experimental conditions on the obtained results has been evaluated and compared with those obtained from SEC-MALS-RI (size exclusion chromatography-MALS-RI...
Source: International Journal of Biological Macromolecules - June 18, 2019 Category: Biochemistry Authors: González-Espinosa Y, Sabagh B, Moldenhauer E, Clarke P, Goycoolea FM Tags: Int J Biol Macromol Source Type: research

Novel natural composite films as packaging materials with enhanced properties.
This study opens the door to use cellulosic composite materials to produce environmentally friendly, low-cost and sustainable packaging materials. PMID: 31226378 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - June 18, 2019 Category: Biochemistry Authors: Mohamed SAA, El-Sakhawy M, Nashy EHA, Othman AM Tags: Int J Biol Macromol Source Type: research

Recycling of viscose yarn waste through one-step extraction of nanocellulose.
In this study, VW was used as raw material to successfully extract nanocellulose (NC) in a facile one-step extraction process, without any pretreatment. Different hydrolysis reaction times (5-60 min) were employed, and the extracted material was characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning and transmission electron microscopies, atomic force microscopy, and thermogravimetric analysis. Interestingly, it was possible to obtain NC in only 5 min of reaction without any pretreatment and with an increase of 50% in the crystallinity, which was attributed to the pretreatments VW undergo...
Source: International Journal of Biological Macromolecules - June 18, 2019 Category: Biochemistry Authors: Prado KS, Gonzales D, Spinacé MAS Tags: Int J Biol Macromol Source Type: research

Assessment of antiherpetic activity of nonsulfated and sulfated polysaccharides from Azadirachta indica.
Abstract Azadirachta indica leaf is used by Indian population for the healing of various diseases including viral infection. Herein, we analyzed the antiherpetic (HSV-1) activity of two polysaccharides (P1 and P2) isolated from the leaf of A. indica and their chemically sulfated derivatives (P1S and P2S). The molecular weights of P1S and P2S are 41 and 11 kDa, respectively. Sulfate groups are located at positions C3 of the Araf and C6 of both Galp and Glcp residues of the most active polysaccharide (P1S). These compounds were not cytotoxic in HEp-2 cells, up to 1000 μg/mL. Both P1S and P2S exhibited antivir...
Source: International Journal of Biological Macromolecules - June 18, 2019 Category: Biochemistry Authors: Faccin-Galhardi LC, Ray S, Lopes N, Ali I, Espada SF, Dos Santos JP, Ray B, Linhares REC, Nozawa C Tags: Int J Biol Macromol Source Type: research

A review on latest innovations in natural gums based hydrogels: Preparations & applications.
A review on latest innovations in natural gums based hydrogels: Preparations & applications. Int J Biol Macromol. 2019 Jun 18;: Authors: Ahmad S, Ahmad M, Manzoor K, Purwar R, Ikram S Abstract The prospective uses of natural gum polysaccharides in various aspects of food, water, energy, biotechnology, environment and medicine industries, have garnered a great deal of attention recently. Natural gums have gained widespread attention due to their availability, low cost, structural diversity and remarkable properties as 'green' bio-based renewable materials. Natural gums are obtainable as natural pol...
Source: International Journal of Biological Macromolecules - June 18, 2019 Category: Biochemistry Authors: Ahmad S, Ahmad M, Manzoor K, Purwar R, Ikram S Tags: Int J Biol Macromol Source Type: research

Enhancement of the properties of biosourced poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by the incorporation of natural orotic acid.
Abstract The aim of this study is to use natural orotic acid (OA) as a sustainable, environmentally friendly additive to improve the crystallization, rheological, thermal, mechanical, and biodegradation properties of bacterially synthesized poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB). OA was found to be an efficient nucleating agent for P34HB, and dramatically enhanced both non-isothermal and isothermal crystallization rates. The incorporation of OA increased nucleation density and decreased spherulite size, but had little effect on the crystalline structure. The rheological properties of the P34HB were g...
Source: International Journal of Biological Macromolecules - June 18, 2019 Category: Biochemistry Authors: Yu Y, Li Y, Han C, Xiao L Tags: Int J Biol Macromol Source Type: research

Improvement of Selenomonas ruminantium β-xylosidase thermal stability by replacing buried free cysteines via site directed mutagenesis.
Improvement of Selenomonas ruminantium β-xylosidase thermal stability by replacing buried free cysteines via site directed mutagenesis. Int J Biol Macromol. 2019 Jun 17;: Authors: Dehnavi E, Moeini S, Akbarzadeh A, Dabirmanesh B, Siadat SOR, Khajeh K Abstract β-xylosidase is an essential enzyme for breakdown of xylan to d-xylose. It has a significant potential application value for medicine, food, paper and pulp, and biofuel industries. Due to the negative consequences caused by buried free cysteine residues, mutational substitution of such residues is often accompanied by a notable increase...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Dehnavi E, Moeini S, Akbarzadeh A, Dabirmanesh B, Siadat SOR, Khajeh K Tags: Int J Biol Macromol Source Type: research

Functionalization of carbon nanotubes with chitosan based on MALI multicomponent reaction for Cu2+ removal.
Abstract In this work, we reported a novel "one-pot" strategy for preparation of chitosan-coated carbon nanotubes (CNTs) composites via a combination of Diels-Alder (DA) reaction and mercaptoacetic acid locking imine (MALI) reaction for the first time. To evaluate the adsorption characteristics, the as-prepared samples were applied to remove copper ions (Cu2+) from aqueous solution. The effects of contact time, solution pH, temperature and initial Cu2+ concentration on the adsorption of Cu2+ onto the as-prepared samples were investigated. The chitosan modified CNTs composites showed high affinity and fas...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Dou J, Gan D, Huang Q, Liu M, Chen J, Deng F, Zhu X, Wen Y, Zhang X, Wei Y Tags: Int J Biol Macromol Source Type: research

Partial characterization and antitumor activity of a polysaccharide isolated from watermelon rinds.
Abstract As a health-beneficial fruit, watermelon is widely consumed by people around the world. However, components responsible for the health benefits are not yet determined. As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits. In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone. The monosaccharide composition revealed that the extracted pol...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Dammak MI, Salem YB, Belaid A, Mansour HB, Hammami S, Le Cerf D, Majdoub H Tags: Int J Biol Macromol Source Type: research

Chicken egg yolk antibody (IgY) as diagnostics and therapeutics in parasitic infections - A review.
Abstract Early diagnosis and treatment of parasitic diseases are indispensable to combat parasites mediated morbidity and mortality in humans and animals. Mammalian sourced antibodies are being successfully used in immunotherapy and immunoassays. However, their increased conservation amongst mammals, involves them in unnecessary interaction and immune mediated pathologies, obstructing their applications in certain approaches in immunoassays. Further, the high production cost and difficulty to achieve high and stable antibody titer hampers their utility for therapeutic purposes. In recent years, chicken egg yolk im...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Diraviyam T, Ambi SV, Vieira-Pires RS, Xiaoying Z, Sekaran S, Krishnan U Tags: Int J Biol Macromol Source Type: research

Comprehensive analysis of lncRNA and mRNA expression profiles in macrophages activated by Actinidia eriantha polysaccharide.
This study further expanded current knowledge on the mechanisms of plant polysaccharides. PMID: 31220493 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Chen X, Du J, Zhu Y, Zhang C, Sun H Tags: Int J Biol Macromol Source Type: research

Efficient hydrolysis of wine and grape juice anthocyanins by Malbranchea pulchella β-glucosidase immobilized on MANAE-agarose and ConA-Sepharose supports.
Efficient hydrolysis of wine and grape juice anthocyanins by Malbranchea pulchella β-glucosidase immobilized on MANAE-agarose and ConA-Sepharose supports. Int J Biol Macromol. 2019 Jun 17;: Authors: Monteiro LMO, Pereira MG, Vici AC, Heinen PR, Buckeridge MS, de Lourdes Teixeira de Moraes Polizeli M Abstract β-glucosidases (BGLs) hydrolyze short-chain cellulooligosaccharides. Some BGLs can hydrolyze anthocyanins and be applied in the clarification process of food industries, especially grape juice and wine. Enzyme immobilization is a valuable tool to increase enzyme stabilization. In this wo...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Monteiro LMO, Pereira MG, Vici AC, Heinen PR, Buckeridge MS, de Lourdes Teixeira de Moraes Polizeli M Tags: Int J Biol Macromol Source Type: research

A porous collagen-carboxymethyl cellulose/hydroxyapatite composite for bone tissue engineering by bi-molecular template method.
Abstract Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules. The morphology, composition and physical properties of Col-CMC/HA composites were characterized systematically. It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm. The porosities of composites were located at the range of 71%-85%. Besides,...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: He X, Tang K, Li X, Wang F, Liu J, Zou F, Yang M, Li M Tags: Int J Biol Macromol Source Type: research

Innovative natural polymer metal nanocomposites and their antimicrobial activity.
Abstract Metal based nanocomposites are gaining popularity for the past few years due to their promising chemical and physical properties. These nanocomposites can be obtained by incorporation of metal nanoparticles with glass, ceramic and polymer. Metal polymer nanoparticles can be formed through direct reduction method, in situ methods like chemical reduction, photoreduction and thermal decomposition of metallic salt inside the polymer, ex-situ by direct insertion of metallic nanoparticles into the polymer, through vapor phase deposition techniques and ion implantation. Natural polymers such as cellulose, starch...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Zahran M, Marei AH Tags: Int J Biol Macromol Source Type: research

Improving physical properties and retrogradation of thermoplastic starch by incorporating agar.
, Móczó J Abstract To develop functional and sustainable packaging materials from starch and to enhance their properties, agar was added to thermoplastic corn starch (TPS) in a wide concentration range and the products were prepared either by casting or melt blending with a high glycerol content. The role of agar in the mechanical and barrier performance of films, as well as the compatibility of TPS and agar was systematically evaluated. In addition, the retrogradiation of starch in various blends after long storage periods was widely characterized. Results proved that the addition of agar to TPS res...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Fekete E, Bella É, Csiszár E, Móczó J Tags: Int J Biol Macromol Source Type: research

Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.
Abstract In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B. Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways. Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 &...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Song S, Wang L, Wang L, Yu Q, Ai C, Fu Y, Yan C, Wen C, Zhu Z Tags: Int J Biol Macromol Source Type: research

Nonmulberry silk protein sericin blend hydrogels for skin tissue regeneration - in vitro and in vivo.
Abstract The damage to the skin is most prominent and evident as it is our first line of defense and unremittingly under attack by biological and environmental factors. The restoration of the skin is dependent on the extent of the injury. To explore the prospects of new biomimetic material, bi-layered skin construct is fabricated in vitro with nonmulberry silk protein sericin and chitosan hydrogels using human dermal fibroblasts and keratinocytes. The in vitro analysis of the hydrogels showed enhanced adhesion, proliferation, and migration of skin cells with coordinated interaction amongst themselves leading to th...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Sapru S, Das S, Mandal M, Ghosh AK, Kundu SC Tags: Int J Biol Macromol Source Type: research

Biologically active novel N, N, O donor tridentate water soluble hydrazide based O-carboxymethyl chitosan Schiff base Cu (II) metal complexes: Synthesis and characterisation.
Abstract Water soluble hydrazide based O-carboxymethyl chitosan Schiff base ligands have been prepared from chitosan and a mixture of benzoic acidhydrazide with β-diketone by carboxymethylation using mono chloroaceticacid (MCA) through in-situ reaction. The degree of deacetylation of chitosan was determined by elemental analysis and potentiometric titration. Schiff base ligands and the functional groups were characterised by FT-IR, 1H, and 13C NMR spectroscopic techniques. The crystallinity of ligands and complexes was evaluated by X-ray powder diffraction (XRD) studies. Thermal stability of all the synthesiz...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Manimohan M, Pugalmani S, Sithique MA Tags: Int J Biol Macromol Source Type: research

Binding and enzymatic properties of Ageritin, a fungal ribotoxin with novel zinc-dependent function.
This report highlights that Ageritin has the structure and function of an RNase but a Mg2+/Zn2+-dependent mechanism of action, a new finding for ribotoxins. As a zinc-dependent toxin, Ageritin can be classified among the arsenal of zinc-binding proteins involved in fungal virulence. PMID: 31220501 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Ruggiero A, García-Ortega L, Moreira M, Ragucci S, Landi N, Di Maro A, Berisio R Tags: Int J Biol Macromol Source Type: research

An alkali-extracted polysaccharide from Zizyphus jujuba cv. Muzao: Structural characterizations and antioxidant activities.
Abstract An acidic polysaccharide (SAZMP3) was isolated from jujube fruit residues by alkaline extraction and purified with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography. The structural characterizations of SAZMP3 were determined by high-performance gelpermeation chromatography, gas chromatography, Fourier transform infrared spectroscopy, methylation analysis, NMR spectroscopy, scanning electron microscopy, and atomic force microscopy. The results determined SAZMP3 was an acidic polysaccharide mainly contained rhamnose, galactose, and galacturonic acid with a molecular weight of 9.37 kDa and...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Lin X, Ji X, Wang M, Yin S, Peng Q Tags: Int J Biol Macromol Source Type: research

Crystallographic structure and molecular dynamics simulations of the major endoglucanase from Xanthomonas campestris pv. campestris shed light on its oligosaccharide products release pattern.
pov I Abstract Cellulases are essential enzymatic components for the transformation of plant biomass into fuels, renewable materials and green chemicals. Here, we determined the crystal structure, pattern of hydrolysis products release, and conducted molecular dynamics simulations of the major endoglucanase from the Xanthomonas campestris pv. campestris (XccCel5A). XccCel5A has a TIM barrel fold with the catalytic site centrally placed in a binding groove surrounded by aromatic side chains. Molecular dynamics simulations show that productive position of the substrate is secured by a network of hydrogen bonds in th...
Source: International Journal of Biological Macromolecules - June 16, 2019 Category: Biochemistry Authors: Puhl AC, Prates ET, Rosseto FR, Manzine LR, Stankovic I, de Araújo SS, Alvarez TM, Squina FM, Skaf MS, Polikarpov I Tags: Int J Biol Macromol Source Type: research

Improved enzymatic hydrolysis of hardwood and cellulase stability by biomass kraft lignin-based polyoxyethylene ether.
Abstract Water-soluble kraft lignin-based polyoxyethylene ether (KL-PEG), synthesized from the black liquor of kraft pulping and PEG, was used to improve the enzymatic hydrolysis efficiency of dilute acid pretreated (DA-pretreated) Eucalyptus hardwood and cellulase stability. The physicochemical properties of KL-PEG polymer such as solubility, surface tension, charge and aggregation behavior in the solution were first studied. KL-PEG could enhance the enzymatic hydrolysis of Avicel and DA-pretreated Eucalyptus from 63.6% and 58.3% to 78.5% and 93.8%, respectively. The enzymatic activity of cellulase after the enzy...
Source: International Journal of Biological Macromolecules - June 16, 2019 Category: Biochemistry Authors: Lin X, Yang Y, Wu L, Wu L, Xu D, Qin Y Tags: Int J Biol Macromol Source Type: research

Adsorption and photocatalysis assisted optimization for drug removal by chitosan-glyoxal/Polyvinylpyrrolidone/MoS2 nanocomposites.
Abstract Chitosan-glyoxal/Polyvinylpyrrolidone/MoS2 (CSG/PVP/MoS2) nanocomposites were synthesized via hydrothermal-ultrasonic method. The CSG/PVP/MoS2 nano catalyst was characterized by XRD, UV-visible spectroscopy, FTIR, SEM and X-ray photoelectron spectroscopy, and diclofenac was used for the adsorption and photo-degradation test. SEM revealed the CSG/PVP/MoS2 nanocomposite has coarse and semi-flake structure and UV-vis absorbance spectra indicated its strong UV light response. Additionally, the CSG/PVP/MoS2 presents enhanced adsorption-photocatalytic activities in comparison to CSG/PVP, and the degradation was...
Source: International Journal of Biological Macromolecules - June 16, 2019 Category: Biochemistry Authors: Li X, Zhang Z, Fakhri A, Gupta VK, Agarwal S Tags: Int J Biol Macromol Source Type: research

Phenolic hydroxyl derived copper alginate microspheres as superior adsorbent for effective adsorption of tetracycline.
In this study, a novel phenolic hydroxyl (bayberry tannin) functionalized copper alginate microspheres adsorbent for the adsorption of tetracycline from contaminated water environment had been synthesized by a facile gelation and solidification process. The structure and chemical properties were characterized by the various analytical methods. The as-prepared adsorbent displayed the excellent adsorption performance with the maximum adsorption capacity of 153.89 mg·g-1, and the equilibrium data were well fitted the Langmuir isotherm model and pseudo-second-order kinetic model. In addition, the thermodynamic values ...
Source: International Journal of Biological Macromolecules - June 15, 2019 Category: Biochemistry Authors: Zhang X, Lin X, He Y, Luo X Tags: Int J Biol Macromol Source Type: research

Chitosan capped ZnO nanoparticles with cell specific apoptosis induction through P53 activation and G2/M arrest in breast cancer cells - In vitro approaches.
Abstract Now a days the well-organized strategy to induce apoptosis in cancer chemotherapy is to produce anti-cancer agent without any side effects is in needy. Hence the present investigation was aimed to explore the anticancer potentials of Amorphophallus paeoniifolius reduced zinc nanoparticles capped with chitosan against MCF 7 cell line (breast cancer cells)and studied for its optical and surface charge properties. The size, shape, dispersion and uniform distribution of biosynthesized zincoxide nanoparticle was examined using Field emission scanning electron microscope (FESEM) and Transmission electron micros...
Source: International Journal of Biological Macromolecules - June 15, 2019 Category: Biochemistry Authors: Anitha J, Selvakumar R, Murugan K Tags: Int J Biol Macromol Source Type: research

Improving stability of biosensor based on covalent immobilization of horseradish peroxidase by γ-aminobutyric acid and application in detection of H2O2.
Improving stability of biosensor based on covalent immobilization of horseradish peroxidase by γ-aminobutyric acid and application in detection of H2O2. Int J Biol Macromol. 2019 Jun 15;: Authors: Feizabadi M, Soleymanpour A, Faridnouri H, Ajloo D Abstract A novel electrochemical biosensor for the determination of hydrogen peroxide (H2O2) has been fabricated through covalently immobilized horseradish peroxidase (HRP) on modified multi walled carbon nanotube (MWCNT) by γ-aminobutyric acid (GABA) on glassy carbon electrode. Using the electrochemical techniques, the electrochemical properties...
Source: International Journal of Biological Macromolecules - June 15, 2019 Category: Biochemistry Authors: Feizabadi M, Soleymanpour A, Faridnouri H, Ajloo D Tags: Int J Biol Macromol Source Type: research

Effect of divalent cations on bovine serum albumin (BSA) and tannic acid interaction and its influence on turbidity and in vitro protein digestibility.
Abstract Tannins, proteins, and divalent cations interactions are important for many processes in the food industry and human and animal nutrition and health. The effect of magnesium, calcium, and manganese on the interaction, turbidity, and in vitro protein digestibility of bovine serum albumin and tannic acid complexes was studied. The divalent cations increase the affinity and influence the enthalpy and entropy changes of the protein and tannin binding. Magnesium maintained the nature of interactions, and calcium and manganese changed the binding mechanism. The factor that most influenced turbidity was the tann...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Kaspchak E, Goedert AC, Mafra LI, Mafra MR Tags: Int J Biol Macromol Source Type: research

Lactoferrin-phenothiazine dye interactions: Thermodynamic and kinetic approach.
In this study, the interactions between methylene blue (MB) or azure A (AZA) dyes and bovine lactoferrin (BLF) were investigated by surface plasmon resonance, fluorescence spectroscopy, and isothermal titration microcalorimetry. Despite the molecular similarities between the dyes, the BLF-AZA binding thermodynamic parameters (ΔGAZAo = -30.50 and ΔHAZAo = 10.8 (kJ·mol-1)) were higher in magnitude than those of the BLF-MB systems (ΔGMBo = -27.3 and ΔHMBo = 5.72 (kJ·mol-1)). To increase the systems' entropy (TΔSAZAo = 41.3 and TΔSMBo = 33.0 (kJ·mol-1)), the hydrophobic ...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Coelho YL, de Paula HMC, Agudelo AJP, Benhame AS, Hudson EA, Pires ACS, Silva LHM Tags: Int J Biol Macromol Source Type: research

Disassociation of β1-α1-β2 from the α2-α3 domain of prion protein (PrP) is a prerequisite for the conformational conversion of PrPC into PrPSc: Driven by the free energy landscape.
Disassociation of β1-α1-β2 from the α2-α3 domain of prion protein (PrP) is a prerequisite for the conformational conversion of PrPC into PrPSc: Driven by the free energy landscape. Int J Biol Macromol. 2019 Jun 14;: Authors: Chandrasekaran P, Santosh Kumar C, Rangachari K, Sekar K Abstract Misfolding of the cellular prion protein (PrPC) into β-sheet-rich scrapie form (PrPSc) is associated with transmissible spongiform encephalopathies. A point mutation F198S is responsible for the development of a rare inherited Gerstmann-Straussler-Scheinker disease caused by the aggr...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Chandrasekaran P, Santosh Kumar C, Rangachari K, Sekar K Tags: Int J Biol Macromol Source Type: research

Chemometrical-electrochemical investigation for comparing inhibitory effects of quercetin and its sulfonamide derivative on human carbonic anhydrase II: Theoretical and experimental evidence.
Abstract This paper reports results of a valuable study on investigation of inhibitory effects of the sulfonamide derivative of quercetin (QD) on human carbonic anhydrase II (CA-II) by electrochemical and chemometrical approaches. To achieve this goal, a glassy carbon electrode (GCE) was chosen as the sensing platform and different electrochemical techniques such as cyclic voltammetry (CV), differential pulse voltammetry (DPV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) were used to investigate and comparing inhibitory effects of quercetin (Q) and QD on CA-II. By the use of EQU...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Khodarahmi R, Khateri S, Adibi H, Nasirian V, Hedayati M, Faramarzi E, Soleimani S, Goicoechea HC, Jalalvand AR Tags: Int J Biol Macromol Source Type: research

Variants at position 603 of the CGTase from Bacillus circulans STB01 for reducing product inhibition.
Abstract Inhibition of cyclodextrin glycosyltransferases (CGTases) by their product cyclodextrins limits the efficiency of cyclodextrin production. In an effort to produce variants with good activity but reduced product inhibition, six mutants were constructed at position 603 of the CGTase from Bacillus circulans STB01, which exhibits mixed-type product inhibition. In a kinetic analysis, N603I showed reduced noncompetitive inhibition while N603K, N603H and N603R showed increased noncompetitive inhibition. Unexpectedly, N603E and N603D exhibited reductions in both competitive and noncompetitive product inhibition. ...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Chen S, Li Z, Gu Z, Hong Y, Cheng L, Li C Tags: Int J Biol Macromol Source Type: research

Pyrimidine biosynthesis in pathogens - Structures and analysis of dihydroorotases from Yersinia pestis and Vibrio cholerae.
Abstract The de novo pyrimidine biosynthesis pathway is essential for the proliferation of many pathogens. One of the pathway enzymes, dihydroorotase (DHO), catalyzes the reversible interconversion of N-carbamoyl-l-aspartate to 4,5-dihydroorotate. The substantial difference between bacterial and mammalian DHOs makes it a promising drug target for disrupting bacterial growth and thus an important candidate to evaluate as a response to antimicrobial resistance on a molecular level. Here, we present two novel three-dimensional structures of DHOs from Yersinia pestis (YpDHO), the plague-causing pathogen, and Vibrio ch...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Lipowska J, Miks CD, Kwon K, Shuvalova L, Zheng H, Lewiński K, Cooper DR, Shabalin IG, Minor W Tags: Int J Biol Macromol Source Type: research

Chondroitin sulfate immobilized PCL nanofibers enhance chondrogenic differentiation of mesenchymal stem cells.
Abstract Cold Atmospheric Plasma (CAP) is used as a promising method in surface modification for immobilization of chondroitin sulfate functional biomacromolecules on PCL nanofibrous substrates for cartilage tissue engineering. The GAG-grafted scaffolds are able to successfully support the attachment and proliferation of mesenchymal stem cells (MSCs). The seeded scaffolds show the chondro-differentiation of MSCs during a 21-days cell culture in a non-differential medium. Expression of SOX9, Collagen10 and Collagen2 proved the chondro-inductive effect of GAG-grafted scaffolds. Besides, no external chondro-genic dif...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Meghdadi M, Pezeshki-Modaress M, Irani S, Atyabi SM, Zandi M Tags: Int J Biol Macromol Source Type: research

The mitochondrial genome of the brown citrus aphid Aphis (Toxoptera) citricidus: Insights into the repeat regions in aphids and phylogenetic implications.
This study provides new insight into the evolution of aphid mt genomes, as well as useful information for resolving aphid phylogenetic questions. PMID: 31207332 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Wei DD, Lang N, Tao Y, He W, Tu YQ, Miao ZQ, Yang L, Wang JJ Tags: Int J Biol Macromol Source Type: research

Low-resolution SAXS and structural dynamics analysis on M. tuberculosis GmhB enzyme involved in GDP-heptose biosynthetic pathway.
Abstract The M. tuberculosis GmhB protein converts the d-glycero-α-d-manno-heptose 1,7-bisphosphate (GMB) intermediate into d-glycero-α-d-manno-heptose 1-phosphate by removing the phosphate group at the C-7 position. To understand the structure and substrate binding mechanism, the MtbGmhB was purified which elutes as monomer on gel filtration column. The small angle x-ray scattering analysis shows that MtbGmhB forms fully folded monomer with shape profile similar to its modeled structure. The circular dichroism analysis shows 38% α-helix, 15% β-sheets and 47% random coil structures in MtbGmh...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Karan S, Pratap B, Ashish, Saxena AK Tags: Int J Biol Macromol Source Type: research

Physicochemical, thermal and mechanical characterization study of perimysial collagen of two bovine muscles.
In this study, chemical, thermal and mechanical characteristics were considered and quantified. However, the mechanical function and development of muscle in-vivo could be the main influence on the extracellular collagen characteristics as well as its interactions with non-collagen compounds. Its formation is essential for muscle function. PMID: 31202843 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Velázquez DE, Latorre ME Tags: Int J Biol Macromol Source Type: research

Structural characterization and immunoregulatory activity of two polysaccharides from the rhizomes of Atractylodes lancea (Thunb.) DC.
Abstract A neutral polysaccharide (ALP-1) and an acidic polysaccharide (ALP-3) were purified from Atractylodes lancea (Thunb.) DC. ALP-1 exhibited a linear backbone composed of (2 → 1)-linked β-d-fructofuranose. The backbone of ALP-3 was elucidated as →4)-GalAp-(1 → 3,4)-Rhap-(1→, and with branch chain substituted at O-3 position of →3,4)-Rhap-(1→. The branch chain consists of →3,5)-Araf-(1→, →5)-Araf-(1→, and Araf-(1→. Particularly, ALP-3 could significantly stimulate macrophage proliferation in a dose-dependent manner, and stimulate phagocytic, ...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Qin J, Wang HY, Zhuang D, Meng FC, Zhang X, Huang H, Lv GP Tags: Int J Biol Macromol Source Type: research

Partial characterization of the edible Spinacia oleracea polysaccharides: Cytoprotective and antioxidant potentials against Cd induced toxicity in HCT116 and HEK293 cells.
Abstract Spinacia is an interesting medicinal halophyte plant that is employed as a food and therapeutic agent in traditional medicine. In this work, water-soluble polysaccharides from Spinacia oleracea were extracted and preliminary characterization was established via FT-IR, UV-vis, 1H NMR and SEC/MALS DRI technics. The extracted polysaccharide, with an average molecular mass of 408 kDa, was composed of arabinose, galactose, mannose, glucose, rhamnose and xylose in the molar percentage of 49.3%, 28.1%, 4.9%, 7.8%, 8.2% and 1.7%, respectively. The polysaccharide showed significant antioxidant activity. Moreover...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Mzoughi Z, Souid G, Timoumi R, Le Cerf D, Majdoub H Tags: Int J Biol Macromol Source Type: research

Calcium and phosphorylation double-regulating caltractin initiating target protein XPC function.
Abstract Human centrin 2 (HsCen2) is a member of the EF-hand protein that plays a critical role in the centrosome duplication and separation during cell division. Reversible protein phosphorylation is an important regulatory mechanism for centrin. To obtain insight into the structural basis for the functional effects of phosphorylation, it was verified that the serine residue at position 170 of HsCen2 can be phosphorylated by protein kinase A (PKA) using 31P NMR spectroscopy. While the protein was phosphorylated, the extents of α-helix and the exposed hydrophobic surfaces on HsCen2 were decreased significant...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Zhao Y, Yang J, Chao J, Yang B Tags: Int J Biol Macromol Source Type: research

Impact of glazing based on chitosan-gelatin incorporated with Persian lime (Citrus latifolia) peel essential oil on quality of rainbow trout fillets stored at superchilled condition.
Abstract In this research, a new ice-glazing based on chitosan-gelatin (Ch-Gl) incorporated with Persian lime peel essential oil (LEO), and superchilled storage was established to assess the microbial, physicochemical, and sensory quality of rainbow trout fillets. The fillets were immersed in distilled water (control), Ch-Gl, Ch-Gl + 1% LEO, and Ch-Gl + 2% LEO to form glazing layer on the surface, then stored at -1.4 °C for 30 days. All treatments delayed the growth of total mesophilic bacteria, total psychrotrophic bacteria, Enterobacteriaceae, and lactic acid bacteria compared to control during t...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Sarmast E, Fallah AA, Dehkordi SH, Rafieian-Kopaei M Tags: Int J Biol Macromol Source Type: research

Thermostability improvement of the glucose oxidase from Aspergillus niger for efficient gluconic acid production via computational design.
In this study, we used in silico design to enhance the stability of glucose oxidase from Aspergillus niger. A combination of the best mutations increased the apparent melting temperature by 8.5 °C and significantly enhanced thermostability and thermoactivation. The variant also showed an increased optimal temperature without compromising the catalytic activity at lower temperatures. Moreover, the combined variant showed higher tolerance at pH 6.0 and 7.0, at which the wild-type enzyme rapidly deactivated. For GA production, an approximate 2-fold higher GA production yield was obtained, in which an almost complete c...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Mu Q, Cui Y, Tian Y, Hu M, Tao Y, Wu B Tags: Int J Biol Macromol Source Type: research

Structure characterization of a novel polysaccharide from Chinese wild fruits (Passiflora foetida) and its immune-enhancing activity.
Abstract A novel polysaccharide (PFP1) with an average molecular weight of 2.02 × 105 g/mol was isolated from Passiflora foetida fruits through hot water extraction, ethanol precipitation and column chromatography. The structure of PFP1 was determined by GPC-MALS-RI, IC, FT-IR, GC-MS and NMR. The structural analysis showed that PFP1 was a heteropolysaccharide and composed of mannose (48.83%), galactose (32.46%), glucose (6.21%), arabinose (5.88%), fructose (2.24%), galacturonic acid (2.20%), xylose (1.17%), fucose (0.17%), ribose (0.05%), and glucuronic acid (0.78%), with a backbone structure of →...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Song Y, Zhu M, Hao H, Deng J, Li M, Sun Y, Yang R, Wang H, Huang R Tags: Int J Biol Macromol Source Type: research

Gamma radiation induced preparation of polyampholyte nanocomposite polymers for removal of Co(II).
Abstract A series of polyampholyte nanocomposite biopolymers, poly(N,N-diallyldimethylammonium chloride-co-acrylamide) grafted on carboxymethylcellulose/iron(III) oxide [P(DADMAC-AAm)CMC/Fe2O3] and poly(N,N-diallyldimethylammonium chloride-co‑sodium acrylate) grafted on carboxymethylcellulose/iron(III) oxide [P(DADMAC-SA)CMC/Fe2O3], was prepared with different molar ratios of anionic groups to cationic groups using gamma irradiation. The grafting properties and swelling behavior were investigated as a function of grafting conditions such as DADMAC, AAm, SA, and CMC concentrations and absorbed dose. Fourier trans...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Elsharma EM, Saleh AS, Abou-Elmagd WSI, Metwally E, Siyam T Tags: Int J Biol Macromol Source Type: research

Overcoming drug-resistance in lung cancer cells by paclitaxel loaded galactoxyloglucan nanoparticles.
This study involves the synthesis of PST-PTX nanoparticles using tamarind seed polysaccharide and Paclitaxel by epichlorohydrin crosslinking. The particles were further characterized by Dynamic Light Scattering (DLS), High-resolution transmission electron microscopy (HR-TEM) Fourier Transform Infrared Spectroscopy (FTIR) and UV-Visible spectroscopy. The cytotoxicity of PST-PTX nanoparticles in cancer cell lines and resistant cancer cell lines were determined by MTT assay. The quantitative analysis of cell death was determined by Annexin V dead cell assay, Caspase 3/7 assay and expression of pro-apoptotic protein Bax. The a...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Reshma PL, Unnikrishnan BS, Preethi GU, Syama HP, Archana MG, Remya K, Shiji R, Sreekutty J, Sreelekha TT Tags: Int J Biol Macromol Source Type: research

Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.
Abstract Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain. The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure. The NMR spectroscopy analysis revealed that EPS was a levan type EPS as a β-(2 → 6)-linked fructan. The FTIR analysis further confirmed the presence of the furanoid rings in the EPS structure. The levan S81 showed high level of thermal stability determined by DSC and TGA analysis. The lyophilised levan S81 showed a sheet-like...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Taylan O, Yilmaz MT, Dertli E Tags: Int J Biol Macromol Source Type: research

Purification, characterization and functional properties of exopolysaccharide from a novel halophilic Natronotalea sambharensis sp. nov.
Abstract An exopolysaccharide producing strain AK103T was isolated from Sambharlake, Rajasthan, India. Based on polyphasic taxonomy, the strain AK103T was found to be a novel species for which the name Natronotalea sambharensis sp. nov. is proposed. The strain was able to secrete (1.2 gL-1) EPS under optimum conditions. The FT-IR spectroscopy revealed that EPS had carboxyl, hydroxyl, carbonyl and sulfate ester groups. The high performance liquid chromatography (HPLC), gel permeation chromatography (GPC) and NMR revealed that the exopolysaccharide was composed of mannose, glucose and glucuronic acid with a molecula...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Singh S, Sran KS, Pinnaka AK, Choudhury AR Tags: Int J Biol Macromol Source Type: research