Chitosan-glyoxal film as a superior adsorbent for two structurally different reactive and acid dyes: Adsorption and mechanism study.
This study reveals that immobilized Chi-Gly film as a good candidate for adsorption of reactive and acid dyes as it does not require any filtration process and adsorbent recovery during and post-adsorption process. PMID: 31150675 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 28, 2019 Category: Biochemistry Authors: Jawad AH, Norrahma SSA, Hameed BH, Ismail K Tags: Int J Biol Macromol Source Type: research

The increased stickiness of non-glutinous rice by alkali soaking and its molecular causes.
This study could broaden the applications of non-glutinous rice by altering its stickiness attribute with alkali soaking. PMID: 31145949 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Zhang N, Wen Y, Yan S, Mao H, Lei N, Li H, Wang J, Chen H, Sun B Tags: Int J Biol Macromol Source Type: research

Study of the cell responses in tantalum carbide nanoparticles-enriched polysaccharide composite hydrogel.
Abstract Salecan is a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp. ZX09. Salecan is suitable for the preparation of hydrogels for biomedical applications, given its prominent physicochemical and biological properties. Herein, we described a novel microwave-assisted polymerized composite hydrogel that was comprised of salecan/poly(diacetone acrylamide) (PDAAm) network and tantalum carbide (TaC) nanoparticles. The incorporation of TaC nanoparticles into the composite hydrogels was confirmed by XRD and EDX analysis, and can be engineered as means for structural reinforcement, th...
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Hu X, Wang Y, Xu M Tags: Int J Biol Macromol Source Type: research

Bacterial cellulose as support for biopolymer stabilized catalytic cobalt nanoparticles.
Abstract In heterogeneous catalysis, ease of separation of the catalyst is considered to be important for its effective re-use. In this paper, we report a synthesis of carboxymethyl cellulose stabilized cobalt nanoparticles (CMC-Co) catalyst and their coating on the bacterial cellulose nanofibers (BCN) as high surface area support to prepare the CMC-Co-BCN dip-catalyst strips. The samples were characterized by scanning electron microscopy for surface morphology, X-ray diffraction spectrometry for crystal structure and thermogravimetric analysis for the thermal behavior and estimation of the Co content in the CMC-C...
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Kamal T, Ahmad I, Khan SB, Asiri AM Tags: Int J Biol Macromol Source Type: research

Effect of soluble soybean polysaccharides on freeze-denaturation and structure of myofibrillar protein of bighead carp surimi with liquid nitrogen freezing.
Abstract This paper investigated the synergistic effect of 3% soluble soybean polysaccharides (SSPS) and liquid nitrogen freezing (-80 °C) on the freezing process and protein denaturation of bighead carp surimi. Freezing curve showed that liquid nitrogen freezing could significantly minimize the elapsed time of maximum-ice-crystal formation zone. Both liquid nitrogen freezing and SSPS were useful in preventing protein denaturation of surimi during 12-week frozen storage. Protein denaturation results indicated that SSPS-LNfreezing surimi1 had the highest protein solubility, Ca2+-ATPase activity and total sulf...
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Gao W, Huang Y, Zeng XA, Brennan MA Tags: Int J Biol Macromol Source Type: research

Experimental and modeling studies of the flow, dynamic and creep recovery properties of pearl millet starch as affected by concentration and cultivar type.
Abstract Starch from pearl millet varieties viz. ProAgro 9444 and HHB 67, selected on the basis of amylose content was studied for steady, dynamic and creep recovery characteristics as affected by concentration (3-15%). ProAgro 9444 and HHB 67 possess amylose content of 20.21% and 15.05%, respectively. Starch gel exhibited shear thinning behavior with flow behavior index  0.969) at all the concentrations. The starch pastes were thixotropic and the extent increased with increasing concentration and amylose content. Dynamic shear properties obtained within the linear viscoelastic region suggested weak gel like beh...
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Bhardwaj M, Sandhu KS, Saxena DC Tags: Int J Biol Macromol Source Type: research

Progressive alterations in crystalline structure of starches during heat-moisture treatment with varying iterations and holding times.
Abstract Corn, tapioca and potato starches after heat-moisture treatment (HMT) at 95-96 °C for 0-60 min with 1-6 iterations were characterized for their gelatinization properties to elucidate the HMT-generated differences in crystalline structure of the starches. The enthalpy changes (ΔH) of all HMT starches were decreased, and the decreases in ΔH of the whole and of the fraction corresponding to well-ordered region of crystalline structure were both in the order of corn 
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Lin CL, Lin JH, Lin JJ, Chang YH Tags: Int J Biol Macromol Source Type: research

Development of resveratrol loaded chitosan-gellan nanofiber as a novel gastrointestinal delivery system.
Abstract Resveratrol loaded chitosan:gellan (CS:Gel) nanofibers (NFs) were prepared for the first time using electrospinning technique. The NFs were prepared with CS (9% w/v) in trifloroacetic acid and Gel (2.5% w/v) at a concentration ratio of 95:5% w/v. Based on the scanning electron microscopy (SEM) images, the diameters of CS-Gel fibers at a ratio of 95:5 and 90:10% w/v were 166 ± 37 and 291 ± 41 nm, respectively. Resveratrol (0.05% w/v) was loaded in NFs of CS-Gel at a ratio of 95:5. Encapsulation efficiency (EE) of resveratrol in NFs of CS-Gel were 86 ± 6%. The amount of re...
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Rostami M, Ghorbani M, Aman-Mohammadi M, Delavar M, Tabibiazar M, Ramezani S Tags: Int J Biol Macromol Source Type: research

Fabrication of magnetic bentonite/carboxymethyl chitosan/sodium alginate hydrogel beads for Cu (II) adsorption.
Abstract In this work, composite hydrogel beads based on magnetic bentonite/carboxymethyl chitosan/sodium alginate (Mag-Ben/CCS/Alg) were fabricated and their ability to adsorb copper ions in water was studied. The structure and properties of the composite were characterized via SEM, FTIR, XPS, TGA, VSM, and Zeta potential tests. The effects of the adsorbent dosage, adsorption time, pH, temperature, and initial concentration of Cu(II) on the adsorption results were studied. The results showed that Mag-Ben/CCS/Alg reached equilibrium after 90 min of adsorption, the removal rate of Cu(II) was 92.62 ± 0....
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Zhang H, Omer AM, Hu Z, Yang LY, Ji C, Ouyang XK Tags: Int J Biol Macromol Source Type: research

Macromolecular pullulan produced by Aureobasidium melanogenum 13-2 isolated from the Taklimakan desert and its crucial roles in resistance to the stress treatments.
Abstract A novel yeast strain Aureobasidium melanogenum 13-2 isolated from the Taklimakan desert was found to be able to produce a high level of extracellular polysaccharide (EPS). Under the optimal conditions, the yeast strain could yield 73.25 ± 2.3 g/L of EPS within 5 days at a flask level. During a 10-liter fermentation, the yeast strain could produce 78.05 ± 3.5 g/L of EPS within 120 h. The FT-IR spectra of the standard pullulan from Sigma and the purified EPS produced by A. melanogenum 13-2 were almost identical and the purified EPS could be actively hydrolyzed by a pullulanase,...
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Jiang H, Chen TJ, Chi Z, Hu Z, Liu GL, Sun Y, Zhang SH, Chi ZM Tags: Int J Biol Macromol Source Type: research

Interface between food grade flavour and water soluble galactan biopolymer to form a stable water-in-oil-in-water emulsion.
Abstract In the present investigation, emulsifying potential of galactan exopolysaccharide (EPS) extracted from Weissella confusa KR780676 has been evaluated with various food grade flavours (vanilla, cardamom and pineapple). Concentration of EPS was optimized as 1% with these flavours, in addition to the effect of salinity (NaCl), monovalent ion (KCl) and temperature on emulsion activity (EA), and emulsion stability (ES) was also inspected. Filter paper wetting test exhibited water-in-oil-in-water (w/o/w) and oil-in-water (o/w) type emulsions. The extent in granule disintegration and the retrogradation process of...
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Kavitake D, Balyan S, Devi PB, Shetty PH Tags: Int J Biol Macromol Source Type: research

Super-swelling lignin-based biopolymer hydrogels for soil water retention from paper industry waste.
Abstract Sources of pulping red liquor, which is produced by acidic sulfite cooking in paper industry, consisted of lignosulphonate and polysaccharide, commonly considered as waste or byproducts and used in low value, such as surfactant and adhesive are wide and its price is low. Here a novel method for high value-added utilization of the pulp and paper industry waste for fabricating super-swelling biopolymer hydrogels is introduced. In contrast to other costly utilizations, which are needed to separate red liquor or purify hemicellulose and lignin before using, it is first time to directly utilize red liquor to p...
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Meng Y, Liu X, Li C, Liu H, Cheng Y, Lu J, Zhang K, Wang H Tags: Int J Biol Macromol Source Type: research

Supramolecular structure and pasting/digestion behaviors of rice starches following concurrent microwave and heat moisture treatment.
Abstract From a supramolecular structure viewpoint, we concern how concurrent microwave and heat moisture treatment (M/HMT) tailors the pasting and digestion features of indica rice starch (IRS) and waxy rice starch (WRS), using combined analytical methods such as scanning electron microscopy and small-angle X-ray scattering. The results confirm that, for the starches (especially IRS), M/HMT led to a concave granule surface, weakened alignment of helices in lamellae, loosened packing of chains in amorphous regions, and somewhat disrupted crystallites. Consequently, the susceptibility of starch structures to hydrot...
Source: International Journal of Biological Macromolecules - May 27, 2019 Category: Biochemistry Authors: Guo Y, Xu T, Li N, Cheng Q, Qiao D, Zhang B, Zhao S, Huang Q, Lin Q Tags: Int J Biol Macromol Source Type: research

Erratum to "Exploring glycogen biosynthesis through Monte Carlo simulation" [Int J Biol Macromol 116 (2018) 264].
Erratum to "Exploring glycogen biosynthesis through Monte Carlo simulation" [Int J Biol Macromol 116 (2018) 264]. Int J Biol Macromol. 2019 May 26;: Authors: Zhang P, Nada SS, Tan X, Deng B, Sullivan MA, Gilbert RG PMID: 31141708 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 26, 2019 Category: Biochemistry Authors: Zhang P, Nada SS, Tan X, Deng B, Sullivan MA, Gilbert RG Tags: Int J Biol Macromol Source Type: research

Antibacterial immunomodulatory and antibiofilm triple effect of Salivaricin LHM against Pseudomonas aeruginosa urinary tract infection model.
Abstract The study sought to purify and characterize a novel bacteriocin from oral L. salivarius and studying the effect of L. salivarius and its bacteriocin against multidrug-resistant (MDR) P. aeruginosa in vivo and in vitro. Saliva Lactobacillus salivarius bacteriocin was prepared and purified. The molecular weight of purified L. salivarius bacteriocin was 13,500 Da protein. The antibacterial activity of purified salivaricin LHM was higher than crude (P 
Source: International Journal of Biological Macromolecules - May 25, 2019 Category: Biochemistry Authors: Mahdi LH, Jabbar HS, Auda IG Tags: Int J Biol Macromol Source Type: research

The structure and properties of natural sheep casing and artificial films prepared from natural collagen with various crosslinking treatments.
Abstract The structure and properties of natural sheep casing and collagen films with various crosslinking treatments have been investigated in detail to develop satisfied artificial casings prepared from collagen. The sheep casing consists of large number of thick collagen fibers oriented at ±45° from longitudinal direction with high-density interwoven network structure. The structural feature of sheep casing gave the special mouthfeel of 'cracking bite' of sausages. Whereas, layered structure filled with fine collagen fibrils and large gaps in collagen film results in poor mechanical properties and hi...
Source: International Journal of Biological Macromolecules - May 25, 2019 Category: Biochemistry Authors: Chen X, Zhou L, Xu H, Yamamoto M, Shinoda M, Tada I, Minami S, Urayama K, Yamane H Tags: Int J Biol Macromol Source Type: research

Effects of polysaccharide from the fruiting bodies of Auricularia auricular on glucose metabolism in 60Co- γ-radiated mice.
This study has focused on the effect of radiation on glucose homeostasis with regard to metabolic function of liver and pancreas and the effect of polysaccharide from the fruiting bodies of Auricularia auricular (SNAAP) on glucose metabolism. The 60Co-γ-radiated mice displayed destroyed redox equilibrium, accompanied by increased blood glucose accumulation, decreased insulin and hepatic glycogen contents, impaired blood glucose tolerance ability, abnormal changes in activities of glucose metabolism-related enzymes and damaged hepatic and pancreatic function, while SNAAP can restore the disordered glucose metabolism t...
Source: International Journal of Biological Macromolecules - May 24, 2019 Category: Biochemistry Authors: Chen Z, Wang J, Fan Z, Qiu J, Rumbani M, Yang X, Zhang H, Wang Z Tags: Int J Biol Macromol Source Type: research

Application of trimethylamine grafted on glyoxal cross-linked chitosan composite for the effective removal of metal ions in batch system.
Abstract This investigation studied the removal of Cu2+, Pb2+, Cd2+, Zn2+, Ni2+ and Cr6+ ions from synthesised wastewater using modified chitosan macromolecules. On this note, chitosan beads (CS) were prepared and cross-linked with glyoxal solution. It was found that cross-linking increased the beads mechanical strength and chemical stability in acid solution and also increased the crystallinity of the beads in the process, which is a shortcoming, as the beads tend to have reduced adsorption capacity. To reduce this shortcoming, the cross-linked chitosan beads (DCS) were grafted with diethylenetriamine. The beads ...
Source: International Journal of Biological Macromolecules - May 24, 2019 Category: Biochemistry Authors: Igberase E, Osifo PO Tags: Int J Biol Macromol Source Type: research

Characterization of disintegrated bacterial cellulose nanofibers/PVA bionanocomposites prepared via ultrasonication.
Abstract With the increasing demand for simple, efficient, environmentally friendly preparation methods to produce cellulose nanofibers for reinforcing a biodegradable film is increased, the role of nanofibers from the pure cellulose produced by bacteria becomes more important. This work characterized bacterial cellulose nanofibers disintegrated using a high shear homogenizer. These nanofibers, in 2.5, 5, and 7.5 mL suspensions, were mixed with PVA gel using ultrasonication. The resulting dried bionanocomposite film was also characterized. Adding nanofiber significantly increases (p ≤ 0.05) on tensile str...
Source: International Journal of Biological Macromolecules - May 24, 2019 Category: Biochemistry Authors: Abral H, Kadriadi, Mahardika M, Handayani D, Sugiarti E Tags: Int J Biol Macromol Source Type: research

Complete mitochondrial genome of Ophichthus brevicaudatus reveals novel gene order and phylogenetic relationships of Anguilliformes.
g L Abstract Generally, a teleostean group possesses only one type or a set of similar mitochondrial gene arrangement. However, two types of gene arrangement have been identified in the mitochondrial genomes (mitogenomes) of Anguilliformes. Here, a newly sequenced mitogenome of Ophichthus brevicaudatus (Anguilliformes; Ophichthidae) was presented. The total length of the O. brevicaudatus mitogenome was 17,773 bp, and it contained 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs), 22 transfer RNA (tRNA) genes, and two identical control regions (CRs). The gene order differed from that of the typical verte...
Source: International Journal of Biological Macromolecules - May 24, 2019 Category: Biochemistry Authors: Lü Z, Zhu K, Jiang H, Lu X, Liu B, Ye Y, Jiang L, Liu L, Gong L Tags: Int J Biol Macromol Source Type: research

Direct immobilization and recovery of recombinant proteins from cell lysates by using EctP1-peptide as a short fusion tag for silica and titania supports.
In this study, EctP1 peptide was explored as a novel short fusion tag for non-covalent adsorption on unmodified solid surfaces, silica and titania. A fusion of EctP1 with bovine carbonic anhydrase (BCA) was employed to investigate the optimal binding conditions that could diminish the nonspecific adsorption of Escherichia coli proteins. The stable binding of BCA-EctP1 on titania was observed in the pH range of 2-9, while the stable binding on silica was in the pH range 6-9. We further demonstrated the merits of the noncovalent immobilization of EctP1 fusion proteins to silica and titania in the recovery of the bound protei...
Source: International Journal of Biological Macromolecules - May 24, 2019 Category: Biochemistry Authors: Kim JK, Abdelhamid MAA, Pack SP Tags: Int J Biol Macromol Source Type: research

Protective effects of mannan/ β-glucans from yeast cell wall on the deoxyniyalenol-induced oxidative stress and autophagy in IPEC-J2 cells.
Protective effects of mannan/β-glucans from yeast cell wall on the deoxyniyalenol-induced oxidative stress and autophagy in IPEC-J2 cells. Int J Biol Macromol. 2019 May 24;: Authors: Guo W, Gu X, Tong Y, Wang X, Wu J, Chang C Abstract The aim of this study was to investigate the effects of biomacromolecules mannan/β-glucans from yeast cell wall (BYCW) to alleviate Deoxynivalenol(DON)-induced injury. Considering that DON has strong oxidizing effect and stimulates autophagy and apoptosis, we examined the effects of BYCW on consequent oxidative stress damage indicators, cells autophagy and apop...
Source: International Journal of Biological Macromolecules - May 24, 2019 Category: Biochemistry Authors: Guo W, Gu X, Tong Y, Wang X, Wu J, Chang C Tags: Int J Biol Macromol Source Type: research

Preparation of pH-sensitive and antioxidant packaging films based on κ-carrageenan and mulberry polyphenolic extract.
In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE). Due to abundant anthocyanin content, MPE showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13. When MPE was incorporated into κ-carrageenan film, the composite films presented blue color. Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carra...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Liu Y, Qin Y, Bai R, Zhang X, Yuan L, Liu J Tags: Int J Biol Macromol Source Type: research

Utility of laccase in pulp and paper industry: A progressive step towards the green technology.
Abstract Laccase has the enormous potential to be implemented as the multitasking biocatalyst in whole process of paper making. The enzyme can utilize effectively for pulping, delignification of pulps as alone or in the combination with other bleaching enzymes. Laccase has been evaluated for the biografting of pulp fibers, decolorize and stabilize the effluent of the paper mills, biotransformation of high molecular weight (HMW) lignins to lower molecular weight (LMW) aromatic compounds. Further this enzyme has the huge possibilities to apply for deinking of old newsprint (ONP) and pitch removal from varieties of t...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Singh G, Arya SK Tags: Int J Biol Macromol Source Type: research

Facile preparation of a collagen-graphene oxide composite: A sensitive and robust electrochemical aptasensor for determining dopamine in biological samples.
Abstract A sensitive and robust electrochemical aptasensor for determining dopamine (DA) was developed using a grass carp skin collagen-graphene oxide (GCSC-GO) composite as a transducer and a label-free aptamer as a biological recognition element for the first time. In order to fabricate this sensor, the GCSC-GO composite was firstly prepared by ultra-sonication method and characterized by atomic force microscope, infrared spectroscopy, Raman spectroscopy, and electrochemical impedance spectroscopy. Subsequently, a label-free DA-binding aptamer was immobilized through strong interaction between collagen and aptam...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Wei B, Zhong H, Wang L, Liu Y, Xu Y, Zhang J, Xu C, He L, Wang H Tags: Int J Biol Macromol Source Type: research

An efficient large-scale refolding technique for recovering biologically active recombinant human FGF-21 from inclusion bodies.
In this study, an efficient method for preparation of FGF-21 was established. This novel process provides an important technical basis for the large-scale production of rhFGF-21. PMID: 31129207 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Ye X, Yu D, Wu Y, Han J, Li S, Wu Q, Li D, Qi J Tags: Int J Biol Macromol Source Type: research

L-amino acid oxidase isolated from Micrurus mipartitus snake venom (MipLAAO) specifically induces apoptosis in acute lymphoblastic leukemia cells mostly via oxidative stress-dependent signaling mechanism.
In conclusion, M. mipartitus venom and MipLAAO selectively induces apoptosis in Jurkat cells through a H2O2-mediated signaling pathway dependent mostly on CASPASE-3 pathway. Our findings support the potential use of M. mipartitus snake venom compounds as a potential treatment for T-ALL. PMID: 31129208 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Bedoya-Medina J, Mendivil-Perez M, Rey-Suarez P, Jimenez-Del-Rio M, Núñez V, Velez-Pardo C Tags: Int J Biol Macromol Source Type: research

The immunotoxin activity of exotoxin A is sensitive to domain modifications.
Abstract Immunotoxins are a class of recombinant proteins which consist of an antibody and a part of a bacterial or herbal toxin. Immunotoxins containing Pseudomonas aeruginosa exotoxin A (PEA) have been found to be very applicable in clinical trials. Many obstacles such as solubility and absorbency reduce their usability in solid tumors. The current study aims to overcome the mentioned barriers by addition and removal of functional and non-functional domains with a structural approach. In the experimental section, we took advantage of molecular dynamics simulations to predict the functionality of candidate immuno...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Tehranizadeh ZA, Sankian M, Bazzaz BSF, Chamani J, Mehri S, Baratian A, Saberi MR Tags: Int J Biol Macromol Source Type: research

Crystal structure of CntK, the cofactor-independent histidine racemase in staphylopine-mediated metal acquisition of Staphylococcus aureus.
Abstract Staphylopine is a newly identified broad-spectrum metallophore for metal acquisition, and it plays important roles in the survival and virulence of Staphylococcus aureus and other pathogens in the metal-scarce environment in hosts. CntK catalyzes the first step of staphylopine synthesis by converting L-histidine to D-histidine to provide an essential building block of staphylopine. Herein, the crystal structures of S. aureus CntK (SaCntK) and its C72S variant are determined at 1.82 and 1.58 Å resolution, respectively. SaCntK forms a homodimer and each subunit contains a two-domain α/β s...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Luo S, Ju Y, Zhou J, Gu Q, Xu J, Zhou H Tags: Int J Biol Macromol Source Type: research

Genomic analysis and adaptive evolution of the RIG-I-like and NOD-like receptors in reptiles.
Abstract Pattern recognition receptors (PRRs) play a crucial role in the host's innate immune system. Among the PPRs, the RIG-I-like (RLRs) and NOD-like receptors (NLRs) are two important subgroups. To understand the role of RLRs (RIG-I, MDA5, LGP2) and NLRs (NOD1, NLRC3, NLRX1, NOD2) in reptilian evolution, we identified six genes from reptilian genomes. A total of 168 putative genes were identified from 28 reptile species, including 141 intact genes, 25 partial genes and two pseudogenes. Interestingly, the NOD2 gene was absent in all reptile species. Phylogenetic results showed that all genes were divided into f...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Chen J, Shang S, Wu X, Zhong H, Zhao C, Wei Q, Zhang H, Xia T, Chen Y, Zhang H, Tang X Tags: Int J Biol Macromol Source Type: research

Ufl1/RCAD, a Ufm1 E3 ligase, has an intricate connection with ER stress.
Abstract Ufmylation is a type of post-translational modification that deals with complex and fine-tuned cellular activities. This modification proceeds mainly through a three-step enzymatic reaction with ubiquitin-fold modifier 1 (Ufm1), ubiquitin-like modifier-activating enzyme 5 (Uba5), Ufm1-conjugating enzyme 1 (Ufc1) and Ufm1-specific ligase 1 (Ufl1). Ufl1 has previously been reported to function as a Ufm1 E3 ligase in the ufmylation system, but knowledge of its physiological functions remains poor. At the subcellular level, Ufl1 is enriched in the endoplasmic reticulum (ER), implying that it may regulate even...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Xie Z, Fang Z, Pan Z Tags: Int J Biol Macromol Source Type: research

Characterization, hypolipidemic and antioxidant activities of degraded polysaccharides from Ganoderma lucidum.
Abstract The aim of this work is to characterize the primary structure and physicochemical properties of natural polysaccharides (GLP) and degraded polysaccharides (GLPUD) from Ganoderma lucidum, and evaluate their hypolipidemic and antioxidant activities. The results of particle size distribution and scanning electron microscopy (SEM) showed that Ganoderma lucidum polysaccharides were effectively degraded by ultrasonic method. GLPUD was composed of the same monosaccharide units as GLP but with different molar ratios. Infrared spectra and NMR showed that the primary structure of polysaccharides had not been change...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Xu Y, Zhang X, Yan XH, Zhang JL, Wang LY, Xue H, Jiang GC, Ma XT, Liu XJ Tags: Int J Biol Macromol Source Type: research

Soft hydrogel based on modified chitosan containing P. granatum peel extract and its nano-forms: Multiparticulate study on chronic wounds treatment.
Abstract Punica granatum peel (PGP) is widely used in traditional medicinal purposes for chronic wounds owing to containing natural phenolics active components. In current study, active wound dressing hydrogel for chronic wound healing was prepared based on P. granatum peel crude extract (PGPC), ethyl acetate fraction (PGPEA) and their silver nanoforms (Ag-NPs). Methacrylated chitosan was synthesized as precursor to hydrogel and crosslinked by divinyl sulfone (DVS) in mild condition. Hydrogel was fully characterized by spectral morphological, mechanical and physical analyses. The integration of PGPEA silver nanofo...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Ragab TIM, Nada AA, Ali EA, Shalaby ASG, Soliman AAF, Emam M, El Raey MA Tags: Int J Biol Macromol Source Type: research

An investigation for recovery of polyhydroxyalkanoates (PHA) from Bacillus sp. BPPI-14 and Bacillus sp. BPPI-19 isolated from plastic waste landfill.
Abstract Bio-plastic synthesis from renewable and cheap agro-based materials is a sustainable solution for replacing conventionally produced plastic with environmental contamination. The current study was aimed at screening and characterization of Polyhydroxyalkanoates (PHA) producing bacterial isolates, evaluation of their potential and recovery of PHA using the isolates. The PHA compounds were characterized using FT-IR. Based on 16SrRNA sequence analyses the isolates were designated as Bacillus sp. BPPI-14 and Bacillus sp. BPPI-19. The isolates were gram-positive, rod-shaped, endospore former, and citrate test p...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Mohammed S, Panda AN, Ray L Tags: Int J Biol Macromol Source Type: research

High-level expression of codon-optimized Thielavia terrestris cutinase suitable for ester biosynthesis and biodegradation.
Abstract A codon-optimized cutinase gene (TtCutopt) from Thielavia terrestris was over-expressed in Pichia pastoris. An extracellular activity reached 10,200 U/mL using high cell density fermentation. The optimal pH and temperature of TtCutopt were 7.0 and 50 °C, respectively. It displayed high stability over a wide range of pH from 3.0 to 11.0 and up to 85 °C. Among tested p-nitrophenyl esters and triglycerides, TtCutopt showed the highest activity towards p-nitrophenyl butyrate and tributyrin, with specificity activity of 2322.4 U/mg and 1152.5 U/mg, respectively. It was extremely stable in org...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Duan X, Jiang Z, Liu Y, Yan Q, Xiang M, Yang S Tags: Int J Biol Macromol Source Type: research

In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.
In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice. Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α). Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut mic...
Source: International Journal of Biological Macromolecules - May 23, 2019 Category: Biochemistry Authors: Zhang N, Wang Y, Kan J, Wu X, Zhang X, Tang S, Sun R, Liu J, Qian C, Jin C Tags: Int J Biol Macromol Source Type: research

The effect of the grafting percent on some of the physico-chemical and thermal properties and the decomposition course of novel starch-g-poly(cinnamyl acrylate) copolymers.
Abstract This paper focuses on the chemical modification of potato starch by the cinnamyl acrylate monomer during the graft copolymerization. The maximum of the grafting percent (G) was found to be 49.2% ± 0.3 at the temperature of 65 °C for 120 min in the presence of 1.0 wt% of potassium persulfate at the starch and monomer ratio 1:2.5. The copolymers exhibited excellent resistance to polar solvents and moisture which was independent of their G values. It was due to the presence of poly(cinnamyl acrylate) chains in their structure and their very compact, non-porous structure. The chemical sta...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Worzakowska M Tags: Int J Biol Macromol Source Type: research

Silylation of TEMPO oxidized nanocellulose from oil palm empty fruit bunch by 3-aminopropyltriethoxysilane.
Abstract Silylated cellulose has been successfully synthesized using TEMPO-oxidized nanocellulose (TEMPO-NC) from oil palm empty fruit bunch and 3-aminopropyltriethoxysilane (APS) in an ethanol/water medium at a low curing temperature of 40 °C as compared to those reported in the literature of above 100 °C. Confirmation of the grafting process can be seen from the new FTIR peaks at 810 cm-1 and 749 cm-1 which are attributed to the SiC stretching and SiC, and new 13C NMR signals at 10.3, 21.7 and 42.7 ppm which are assigned to C7, C8, and C9 of the silylated TEMPO-NC. The decrease in the intensiti...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Indarti E, Marwan, Rohaizu R, Wanrosli WD Tags: Int J Biol Macromol Source Type: research

Chitosan - Whey protein as efficient delivery system for squalene: Characterization and functional food application.
Abstract Squalene, a triterpenoid compound possessing excellent bioactivities, is not being utilized as a functional food ingredient due to its high susceptibility to oxidation. In the present study, the feasibility of chitosan-whey protein as an efficient wall material for squalene encapsulation using spray drying technique was attempted for functional food applications. The encapsulation efficiency of the squalene powder was found to be 75.4 ± 0.22% whereas other physico-chemical properties such as moisture content, flowability, solubility, peroxide value, etc. have shown satisfactory results. The the...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Lekshmi RGK, Rahima M, Chatterjee NS, Tejpal CS, Anas KK, Vishnu KV, Sarika K, Asha KK, Anandan R, Suseela M Tags: Int J Biol Macromol Source Type: research

An antibiotic (sulfamethoxazole) stabilizes polypeptide (human serum albumin) even under extreme condition (elevated temperature).
Abstract Since aggregation of protein result into number of human diseases including diabetes mellitus, Huntington's and Alzheimer's disease, etc. Hence prevention of aggregation of a polypeptide is of great clinical importance. Human serum albumin (HSA) being major transporter serum protein was studied here in order to prevent its aggregation under extreme conditions. Sulfamethoxazole (SMZ) which is an antibiotic, caused significant inhibition of aggregation which was evident by number of biophysical techniques. Molecular docking was performed to elucidate the protein ligand binding site. In the presence of SMZ d...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Furkan M, Sidddiqi K, Khan AN, Khan RH Tags: Int J Biol Macromol Source Type: research

Protein misfolding, aggregation and mechanism of amyloid cytotoxicity: An overview and therapeutic strategies to inhibit aggregation.
Abstract Protein and peptides are converted from their soluble forms into highly ordered fibrillar aggregates under various conditions inside the cell. Such transitions confer diverse neurodegenerative diseases including Alzheimer's disease, Huntington's disease Prion's disease, Parkinson's disease, polyQ and share abnormal folding of potentially cytotoxic protein species linked with degeneration and death of precise neuronal populations. Presently, major advances are made to understand and get detailed insight into the structural basis and mechanism of amyloid formation, cytotoxicity and therapeutic approaches to...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Zaman M, Khan AN, Wahiduzzaman, Zakariya SM, Khan RH Tags: Int J Biol Macromol Source Type: research

Insight into ponatinib resistance mechanisms in rhabdomyosarcoma caused by the mutations in FGFR4 tyrosine kinase using molecular modeling strategies.
Abstract Novel efficacious treatment of Rhabdomyosarcoma (RMS) with less toxicity has yet to emerge. Genomic analysis of RMS has reported that the receptor tyrosine kinase FGFR4 is highly expressed and frequently mutated in the tumor tissue. The V550E/L and N535D/K mutations of FGFR4 in RMS can lead to strong drug resistance to almost all of the type-I inhibitors. Previous report has demonstrated the type-II inhibitor ponatinib is the most potentially effective agent for RMS but still hard to starboard the V550E/L mutants. In this case, an ensemble of molecular modeling strategies was employed to theoretically unc...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Wu C, Chen X, Chen D, Xia Q, Liu Z, Li F, Yan Y, Cai Y Tags: Int J Biol Macromol Source Type: research

Ovalbumin and guar gum foam and its surface properties as influenced by sucrose and sorbitol.
Abstract The surface properties and foaming of ovalbumin and guar gum aqueous solutions was investigated in the presence of sucrose or sorbitol. All solutions had a broad particle size distribution (395.60 at 1137.50 nm). Higher ovalbumin concentrations had lower equilibrium surface tension and higher absolute values of the zeta potential, regardless the presence of sucrose or sorbitol. Mixtures containing ovalbumin and guar gum resulted in a predominantly elastic character of the air-water interface probably due to the formation of a complex (hydrogen bonding and/or hydrophobic interactions) between ovalbumin a...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Simiqueli AA, Vidigal MCTR, Minim VPR, Minim LA Tags: Int J Biol Macromol Source Type: research

Biosynthesis and statistical optimization of polyhydroxyalkanoate (PHA) produced by Bacillus cereus VIT-SSR1 and fabrication of biopolymer films for sustained drug release.
Abstract Polyhydroxyalkanoates are a fascinating group of biological polymers, which have attracted extensive attention. An effectual PHB accumulating Bacillus cereus strain VIT-SSR1 was isolated from industrial effluent contaminated site. Plackett-Burman design was used to screen the critical factors affecting PHA production. Response surface method was employed to evaluate the interactive effects between the independent variables. The Central composite design was performed to optimize the concentrations of Molasses, ammonium sulphate and initial pH. A PHA content of (1.42 ± 0.01) g/L and a maximum PHA...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Evangeline S, Sridharan TB Tags: Int J Biol Macromol Source Type: research

Preparation and antioxidant activity of sodium alginate and carboxymethyl cellulose edible films with epigallocatechin gallate.
Abstract The active edible films were prepared by incorporating epigallocatechin gallate (EGCG) into sodium alginate (SA) and carboxymethyl cellulose (CMC). The effects of EGCG addition on the physical and morphological properties of SA-CMC films were evaluated. Meanwhile, the release characteristics of EGCG from edible films and its antioxidant activity in fat food simulant (95% ethanol) were also detected. The results showed that the addition of EGCG could improve the tensile strength (TS) of edible films and reduce their elongation at break (E). Especially, the color of edible film added with EGCG deepened and ...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Ruan C, Zhang Y, Wang J, Sun Y, Gao X, Xiong G, Liang J Tags: Int J Biol Macromol Source Type: research

Chitosan-TiO2 microparticles LBL immobilized nanofibrous mats via electrospraying for antibacterial applications.
In this study, chitosan (CS) and Titanium dioxide (TiO2) microparticles were well immobilized on polylactic acid (PLA) mats by electrospinning-electrospraying hybrid technique. The surface morphology, chemical composition and characteristic group of the mats were characterized. The results indicated that CS/TiO2 microparticles were successfully immobilized on the surface of PLA mats. In addition, the antibacterial activity and cytotoxicity of the composite mats were investigated to confirm that the layer-by-layer immobilization of CS/TiO2 microparticles via electrospraying could enhance the antibacterial effect and biocomp...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Dai F, Huang J, Liao W, Li D, Wu Y, Huang J, Long Y, Yuan M, Xiang W, Tao F, Cheng Y, Deng H Tags: Int J Biol Macromol Source Type: research

Grafting of chitosan-acrylamide hybrid on the wool: Characterization, reactive dyeing, antioxidant and antibacterial studies.
This study presents a new approach to enhance reactive dye uptake and functional finishing of wool yarns via simple grafting with synthesized chitosan-acrylamide (Ch-Ac) hybrid. To this, Ch-Ac was synthesized and characterized with fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques. Then, Ch-Ac was grafted on wool, characterized with FTIR, SEM, and weight gain analysis and dyeability with two commercial reactive dyes. Results showed that Ch-Ac treated wool could be dyed at lower temperatures (ca. 40 °C), times (ca. 30 min), and amount of reactiv...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Sadeghi-Kiakhani M, Safapour S, Ghanbari-Adivi F Tags: Int J Biol Macromol Source Type: research

Structural characterization and properties of polyols plasticized chitosan films.
In this study, the influence of different polyols such as glycerol, xylitol and maltitol on the crystalline structure and thermal properties of chitosan films were investigated by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The concentration of polyol was fixed at 20 wt%. FTIR result showed that the addition of polyols weakened the hydrogen bonding between chitosan molecules, whereas the electrostatic interactions remained nearly unchanged. Structural analysis revealed that the plasticizer which promoted the crysta...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Ma X, Qiao C, Wang X, Yao J, Xu J Tags: Int J Biol Macromol Source Type: research

Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli.
Abstract Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles. The PAC-CHT NPs were characterized by size, polydispersity, surface charge, morphology, and PAC content. PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC. Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydis...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Alfaro-Viquez E, Esquivel-Alvarado D, Madrigal-Carballo S, Krueger CG, Reed JD Tags: Int J Biol Macromol Source Type: research

Physicochemical characteristics and biological activities of polysaccharides from the leaves of different loquat (Eriobotrya japonica) cultivars.
Abstract In order to explore polysaccharides from loquat leaves (LLPs) as functional food ingredients for prevention and treatment of obesity and type 2 diabetes, the physicochemical characteristics, in vitro binding properties, and inhibitory effects on α-amylase and α-glucosidase of polysaccharides from the leaves of different loquat cultivars, including 'Baiyu', 'Chuannong8', 'Yuanbao', and 'Dawuxing', were investigated and compared. Results showed that the contents of total uronic acids and degree of esterification in LLPs ranged from (27.04 ± 1.76)% to (41.46 ± 1.91)%, and fr...
Source: International Journal of Biological Macromolecules - May 22, 2019 Category: Biochemistry Authors: Fu Y, Yuan Q, Lin S, Liu W, Du G, Zhao L, Zhang Q, Lin DR, Liu YT, Qin W, Li DQ, Wu DT Tags: Int J Biol Macromol Source Type: research