Real time quantification of intracellular nickel using genetically encoded FRET-based nanosensor.
Abstract Nickel (Ni2+) is an essential mineral nutrient that is required in trace quantities, but exposure to excess amounts can be toxic or carcinogenic. Mechanisms underlying Ni2+ imbalance and toxicity are poorly understood because most analytical methods currently used to probe this metal are invasive or also have toxic effects. To address these problems, a genetically encoded FRET-based probe for nickel metal (FProNiM) was constructed for real-time detection of Ni2+ in live cells. FProNiM was constructed by sandwiching the bacterial periplasmic nickel-binding protein NikA between the fluorescent protein varia...
Source: International Journal of Biological Macromolecules - July 19, 2019 Category: Biochemistry Authors: Soleja N, Mohsin M Tags: Int J Biol Macromol Source Type: research

Physicochemical, antioxidant properties of giant croaker (Nibea japonica) swim bladders collagen and wound healing evaluation.
Abstract Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were obtained from Nibea japonica swim bladders. The denaturation temperature (Td) of ASC and PSC was approximately 33.8 °C. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy (FTIR) analyses indicated that ASC and PSC contained triple-helical type I collagen when compared to rat tail collagen type I. Moreover, the microstructure of collagen sponges was uniform and porous. In addition, ASC and PSC exhibited antioxidant properties and in vitro scratch assays showed that ...
Source: International Journal of Biological Macromolecules - July 19, 2019 Category: Biochemistry Authors: Chen Y, Jin H, Yang F, Jin S, Liu C, Zhang L, Huang J, Wang S, Yan Z, Cai X, Zhao R, Yu F, Yang Z, Ding G, Tang Y Tags: Int J Biol Macromol Source Type: research

Characterization of a d-tagatose 3-epimerase from Caballeronia fortuita and its application in rare sugar production.
Abstract Recently, rare sugars have caused extensively attention due to their beneficial physiological functions and potential applications in food systems and medical fields. Ketose 3-epimerase (KEase) can catalyze reversibly the epimerization between ketoses which is the pivotal enzyme in Izumoring strategy and an effective tool for biological production of rare sugars. In this work, a KEase from Caballeronia fortuita was recombined and characterized as a d-tagatose 3-epimerase (DTEase, EC 5.1.3.31). The recombinant DTEase displayed the highest activity at pH 7.5 and 65 °C in the presence of Co2+. The re...
Source: International Journal of Biological Macromolecules - July 19, 2019 Category: Biochemistry Authors: Li S, Chen Z, Zhang W, Guang C, Mu W Tags: Int J Biol Macromol Source Type: research

The enhancing effect of N-acetylcysteine modified hyaluronic acid-octadecylamine micelles on the oral absorption of paclitaxel.
Abstract A micelle system based on hyaluronic acid (HA)-octadecylamine (OA) conjugate (HOA) functionalized with N-acetylcysteine (NAC) was constructed to yield NAC modified HOA conjugate (NHOA) for improving oral paclitaxel (PTX) delivery (PTX-NHOA). The average size of spherical PTX-NHOA micelles was 162.7 nm with a zeta potential of -27.6 mv. The encapsulation efficiency (EE) and drug loading (DL) of PTX-NHOA micelles were 92.64% and 6.96%, respectively. Additionally, NHOA micelles exihibited significantly higher cellular uptake in comparison with HOA micelles by caveolin-mediated and clathrin-mediated endoc...
Source: International Journal of Biological Macromolecules - July 19, 2019 Category: Biochemistry Authors: Zhang M, Asghar S, Jin X, Hu Z, Ping Q, Chen Z, Shao F, Xiao Y Tags: Int J Biol Macromol Source Type: research

Cellular demolition: Proteins as molecular players of programmed cell death.
Abstract Apoptosis, a well-characterized and regulated cell death programme in eukaryotes plays a fundamental role in developing or later-life periods to dispose of unwanted cells to maintain typical tissue architecture, homeostasis in a spatiotemporal manner. This silent cellular death occurs without affecting any neighboring cells/tissue and avoids triggering of immunological response. Furthermore, diminished forms of apoptosis result in cancer and autoimmune diseases, whereas unregulated apoptosis may also lead to the development of a myriad of neurodegenerative diseases. Unraveling the mechanistic events in de...
Source: International Journal of Biological Macromolecules - July 19, 2019 Category: Biochemistry Authors: Dhuriya YK, Sharma D, Naik AA Tags: Int J Biol Macromol Source Type: research

Application of high-pressure homogenization for improving the physicochemical, functional and rheological properties of myofibrillar protein.
Abstract The present work investigated effects of high-pressure homogenization (HPH) pressure (0, 40, 80 and 120 MPa) on physicochemical, functional and rheological properties of clam myofibrillar protein (CMP). Results showed that HPH changed the CMP secondary and tertiary structures. Absolute zeta potential and protein solubility increased but particle size and turbidity of CMP decreased after HPH treatment. Both of emulsifying properties and foaming properties were significantly improved. The shear stress, apparent viscosity and the viscosity coefficients reduced, but flow index increased. Application of HPH ...
Source: International Journal of Biological Macromolecules - July 18, 2019 Category: Biochemistry Authors: Shi X, Zou H, Sun S, Lu Z, Zhang T, Gao J, Yu C Tags: Int J Biol Macromol Source Type: research

Bioassay of ovarian cancer tumor protein CA 125 using novel nano-ink on the flexible paper substrate: A new platform for efficient diagnosis of cancer.
Abstract Ovarian cancer is the first and most important cause of malignancy death in women. Mucin 16 or MUC16 protein also known as carcinoma antigen 125 (CA 125) is the most commonly used glycoprotein for early stage diagnosis of ovarian cancer. In this work, a novel paper-based bio-device through hand writing of Ag/RGO (silver nanoparticles/reduced graphene oxide) nano-ink on the flexible paper substrate using pen-on-paper technology was developed. The prepared interface was used to the recognition of CA 125 protein in human biofluid. For this purpose, Ag/rGO nano-ink was synthesized by deposition of Ag nanopart...
Source: International Journal of Biological Macromolecules - July 18, 2019 Category: Biochemistry Authors: Nami F, Saadati A, Hassanpour S, Hasanzadeh M, Hassanzadeh A Tags: Int J Biol Macromol Source Type: research

Hyaluronic acid coated electrospun chitosan-based nanofibers prepared by simultaneous stabilizing and coating.
Abstract Chitosan (CS) and hyaluronic acid (HA) are two oppositely charged natural polysaccharides used widely for preparation of nanofibrous scaffolds for tissue engineering applications. Here, we prepared composite fibers composed of CS and HA by electrospinning and subsequent coating. In this regard, two approaches were applied for coating CS nanofibers by HA. In the first method, electrospun nanofiner was first neutralized and then coating was done (HA/CS1), while in the second approach, neutralization and coating were carried out simultaneously (HA/CS2). The overall fibrous structure of the mat was preserved ...
Source: International Journal of Biological Macromolecules - July 18, 2019 Category: Biochemistry Authors: Bazmandeh AZ, Mirzaei E, Ghasemi Y, Kouhbanani MAJ Tags: Int J Biol Macromol Source Type: research

Physicochemical mechanisms of different biopolymer's (lysozyme, gum arabic, whey protein, chitosan) adsorption on green tea extract loaded liposomes.
In this study, polyphenol-rich green tea extract was encapsulated into liposomes by means of microfluidization in two different aqueous media (pH = 3.8 acetate buffer and pH = 6.5 distilled water). Antioxidant loaded vesicles were further mixed with anionic biopolymers (gum arabic, whey protein) and cationic biopolymers (lysozyme, chitosan) separately. The physical and chemical interactions between liposomes and biopolymers were rationalized by particle size, zeta potential, transmission electron microscopy, total phenolic content and antioxidant activity measurements during 28-days storage at 4 °C. Experimen...
Source: International Journal of Biological Macromolecules - July 17, 2019 Category: Biochemistry Authors: Dag D, Guner S, Oztop MH Tags: Int J Biol Macromol Source Type: research

Immobilized lignin peroxidase on Fe3O4@SiO2@polydopamine nanoparticles for degradation of organic pollutants.
Abstract Lignin peroxidase (LiP) was obtained from Pichia methanolica through heterologous expression. LiP was extracted, purified, and immobilized on Fe3O4@SiO2@polydopamine (PDA) nanoparticles to acquire immobilized LiP. The optimal preparation conditions for immobilized LiP were investigated. Results showed that the immobilization efficiency of immobilized LiP reached 56.37% when the enzyme amount, PDA concentration, and immobilization time were 12 mg, 1.6 mg/mL, and 12 h, respectively. Compared with free LiP, the immobilized LiP showed good thermal stability and storage stability and improved pH toleranc...
Source: International Journal of Biological Macromolecules - July 17, 2019 Category: Biochemistry Authors: Guo J, Liu X, Zhang X, Wu J, Chai C, Ma D, Chen Q, Xiang D, Ge W Tags: Int J Biol Macromol Source Type: research

Refolding of thermally denatured cholesterol oxidases by magnetic nanoparticles.
Abstract Proteins are prone to unfolding and subsequent denaturation by changes in temperature, pH and other harsh conditions. Nanoparticles act as artificial 'chaperones' due to favourable orientation of the proteins on their scaffold which prevents aggregation and reconfigures denatured proteins into their native functional state. In the present study, thermal denaturation of Cholesterol oxidases (ChOx) from Pseudomonas aeruginosa PseA, Rhodococcus erythropolis MTCC 3951 and Streptomyces sp. were studied at temperatures 50-70 °C. Further these thermally denatured proteins were refolded using functionalized...
Source: International Journal of Biological Macromolecules - July 17, 2019 Category: Biochemistry Authors: Ghosh S, Ahmad R, Khare SK Tags: Int J Biol Macromol Source Type: research

Crystal structure of a maltooligosaccharide-forming amylase from Bacillus stearothermophilus STB04.
Abstract To better understand structure-function relationships, an X-ray crystal structure of the maltooligosaccharide-forming amylase from Bacillus stearothermophilus STB04 (Bst-MFA) with bound acarbose has been determined at 2.2 Å. The structure revealed a classical three-domain fold stabilized by four calcium ions, in which CaI-CaIII form an unprecedented linear metal triad in the interior of domain B. Catalytic residues are deduced to be two aspartic acids and one glutamic acid (Asp234, Glu264, Asp331), and the acarbose is bound to surrounding amino acid residues, mainly through extensive hydrogen bond...
Source: International Journal of Biological Macromolecules - July 17, 2019 Category: Biochemistry Authors: Xie X, Li Y, Ban X, Zhang Z, Gu Z, Li C, Hong Y, Cheng L, Jin T, Li Z Tags: Int J Biol Macromol Source Type: research

N-glycans of bovine submaxillary mucin contain core-fucosylated and sulfated glycans but not sialylated glycans.
This study is the first confirmation of N-glycan attachment to BSM. PMID: 31325506 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 17, 2019 Category: Biochemistry Authors: Kim J, Lee J, Jang Y, Ha J, Kim D, Ji M, Lee YK, Kim W, You S, Do J, Ryu C, Kim HH Tags: Int J Biol Macromol Source Type: research

Normal expression of KCNJ11 is maintained by the G-quadruplex.
In conclusion, our study showed that the G-quadruplex structure affects microRNA binding to its target mRNA. Thus, our study reveals a novel mechanism for G-quadruplex-dependent regulation of microRNA-mRNA interaction, which is essential to maintain normal gene expression. PMID: 31325507 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 17, 2019 Category: Biochemistry Authors: Zhang J, Wang J, Li F, Zhu M, Wang S, Cui Q, Yuan G, Zhou J, Xu M Tags: Int J Biol Macromol Source Type: research

One pot synthesis of α-amylase metal organic framework (MOF)-sponge via dip-coating technique.
One pot synthesis of α-amylase metal organic framework (MOF)-sponge via dip-coating technique. Int J Biol Macromol. 2019 Jul 17;: Authors: Nadar SS, Rathod VK Abstract Enzyme embedded metal-organic frameworks (MOFs) are blooming in the field of enzyme immobilization on account of their superior catalytic efficiency, thermal and chemical stability as compared to native enzyme. However, the separation and recovery of enzyme embedded MOF composite is quite challenging due to its nanometer size, high dispersity and low density which limits the reusability. In this work, α-amylase embedded ZIF-...
Source: International Journal of Biological Macromolecules - July 17, 2019 Category: Biochemistry Authors: Nadar SS, Rathod VK Tags: Int J Biol Macromol Source Type: research

Anticancer altretamine recognition by bovine serum albumin and its role as inhibitor of fibril formation: Biophysical insights.
Abstract Binding of anticancer drug altretamine with bovine serum albumin (BSA) and its inhibitory effect on fibrillation of the protein has been studied by using a combination of spectroscopic and calorimetric methods. Altretamine is observed to bind with BSA with a moderate binding affinity of the order of 105, which is weakly temperature dependent. Circular dichroism, fluorescence spectroscopic and dynamic light scattering methods have been employed to monitor the conformational change in the protein. Time correlated single photon counting measurements have confirmed ground state complexation of the drug with t...
Source: International Journal of Biological Macromolecules - July 16, 2019 Category: Biochemistry Authors: Ghosh R, Bharathkar SK, Kishore N Tags: Int J Biol Macromol Source Type: research

Integrative analysis reveals evolutionary patterns and potential functions of SWEET transporters in Euphorbiaceae.
This study provides direct evidence for the first time for internal duplication in Euphorbiaceae. The large-scale duplication events represented the main driving force for SWEET family expansion in Euphorbiaceae. In addition, we determined the key VfSWEETs for sucrose transport from source to sink tissues in V. fordii and proposed a possible sucrose transport model, which would be helpful for understanding the mechanism of sucrose transport in V. fordii. This study provided a new insight into the evolution, expression and structural variations of SWEETs in V. fordii and four other Euphorbiaceae. PMID: 31323266 [PubMed...
Source: International Journal of Biological Macromolecules - July 16, 2019 Category: Biochemistry Authors: Cao Y, Liu W, Zhao Q, Long H, Li Z, Liu M, Zhou X, Zhang L Tags: Int J Biol Macromol Source Type: research

Comparative study between homo-metallic & hetero-metallic nanostructures based agar in catalytic degradation of dyes.
Comparative study between homo-metallic & hetero-metallic nanostructures based agar in catalytic degradation of dyes. Int J Biol Macromol. 2019 Jul 16;: Authors: Emam HE, Ahmed HB Abstract Herein, comparison between eco-friendly homo-metallic (Ag) and hetero-metallic (AgAu and Ag-Au-Pd) nanostructures in catalysis was studied. The homo-metallic and hetero-metallic nanostructures were greenly synthesized using Agar as nano-generator. The sequential reduction of metal ions to give homo- and hetero-metallic nanostructures was initially confirmed via UV-Visible spectra. Tracking of transmission electr...
Source: International Journal of Biological Macromolecules - July 16, 2019 Category: Biochemistry Authors: Emam HE, Ahmed HB Tags: Int J Biol Macromol Source Type: research

Enhanced properties of injectable chitosan-based thermogelling hydrogels by silk fibroin and longan seed extract for bone tissue engineering.
In this study, the injectable thermogelling hydrogels under physiological temperature were prepared using chitosan (CS) and silk fibroin (SF). These hydrogels were loaded with crude water extract of longan seed (WLS) to improve osteoblast differentiation and stimulate mineralization in bone cells. These hydrogels showed a sol-to-gel transition time at 37 °C approximately 4-10 min and the formation of microstructure with small slender pores. The cumulative released amount of both GA and EA from the CS hydrogels was much lower than that from the CS/SF hydrogels. In addition, these hydrogels showed non-cytotoxic to bo...
Source: International Journal of Biological Macromolecules - July 16, 2019 Category: Biochemistry Authors: Pankongadisak P, Suwantong O Tags: Int J Biol Macromol Source Type: research

Heterologous expression and characterization of a novel subtilisin-like protease from a thermophilic Thermus thermophilus HB8.
Abstract Subtilisins are a family of serine proteases used widely throughout the detergent, leather and food industries, with the identification and development of new enzymes holding much potential value. Thermus thermophilus HB8 was examined for serine proteases and found TTHA0724 gene. Sequence analysis of this putative serine protease placed it within the subtilisin family. To obtain active T. thermophilus HB8 subtilisins, three genes encoding prepro-subtilisin, pro-subtilisin and mature-subtilisin were cloned and expressed in Escherichia coli Transetta (DE3). Although direct expression of the mature-subtilisi...
Source: International Journal of Biological Macromolecules - July 16, 2019 Category: Biochemistry Authors: Xie G, Shao Z, Zong L, Li X, Cong D, Huo R Tags: Int J Biol Macromol Source Type: research

Topoisomerase inhibition and albumin interaction studies of acridine-thiosemicarbazone derivatives.
Abstract In the present study, acridine-thiosemicarbazones (ATD) derivatives were tested for their interaction properties with BSA through UV-Vis absorption and fluorescence spectroscopic studies. Both hyperchromic and hypochromic effects, as well as red or blue shifts were demonstrated after the derivatives were added to the BSA. Values for the binding constant (Kb) ranged from 1.62 × 104 to 8.71 × 105 M-1 and quenching constant (KSV) from 3.46 × 102 to 7.83 × 103 M-1 indicating a good affinity to BSA protein. Complementary, two compounds were selected to assess their i...
Source: International Journal of Biological Macromolecules - July 16, 2019 Category: Biochemistry Authors: da Silva Filho FA, de Freitas Souza T, Ribeiro AG, Alves JEF, de Oliveira JF, de Lima Souza TRC, de Moura RO, do Carmo Alves de Lima M, de Carvalho Junior LB, de Almeida SMV Tags: Int J Biol Macromol Source Type: research

Comparative mitogenomic analysis of species in the subfamily Amphinemurinae (Plecoptera: Nemouridae) reveal conserved mitochondrial genome organization.
Abstract The subfamily Amphinemurinae has five genera in China, with each genus of similar morphology. To gain a better understanding of architecture and evolution of mitogenome in Amphinemurinae, mitogenomes of eight species representing four genera (Amphinemura, Indonemoura, Protonemura and Sphaeronemoura) in the subfamily Amphinemurinae were sequenced, and a comparative mitogenomic analysis of five genera (including a published stonefly genus, Mesonemoura) was carried out. By comparative analysis, we found highly conserved genome organization of ten Amphinemurinae species including genome contents, gene order, ...
Source: International Journal of Biological Macromolecules - July 15, 2019 Category: Biochemistry Authors: Cao J, Wang Y, Li W Tags: Int J Biol Macromol Source Type: research

Recent developments in the detection of bovine serum albumin.
Abstract Albumin is a globular protein which plays a pivotal role in maintaining plasma pressure and the nutritional balance. Different compounds are transported by binding to albumin in the blood. Also, human health is closely related to the serum albumin concentration in blood plasma or other biological fluids. Due to the high structural similarity with human serum albumin (HSA), bovine serum albumin (BSA) has been widely investigated as a model protein in different fields. Importantly, albumin detection has recently gained huge interest, as this protein serves as an important indicator of cow health, and its mi...
Source: International Journal of Biological Macromolecules - July 15, 2019 Category: Biochemistry Authors: Jahanban-Esfahlan A, Ostadrahimi A, Jahanban-Esfahlan R, Roufegarinejad L, Tabibiazar M, Amarowicz R Tags: Int J Biol Macromol Source Type: research

In vivo and in vitro characterization of hydrophilic protein tag-fused Ralstonia eutropha polyhydroxyalkanoate synthase.
This study aimed to improve the solubility of Ralstonia eutropha PHA synthase (PhaCRe) by fusing a hydrophilic tag, glutathione S-transferase (GST), to the protein's N-terminus. In in vivo assays, the GST tag had no obvious effect on solubility and enzymatic activity of PhaCRe. However, an in vitro assay revealed that the surface of GST-fused PhaCRe (GST-PhaCRe) had increased hydrophilicity, and tended to form correct PhaCRe dimers when added to the (R)-3-hydroxybutyryl-CoA substrate. Although GST-PhaCRe displayed a long lag phase at the start of a polymerization reaction, granule-associated GST-PhaCRe showed higher cataly...
Source: International Journal of Biological Macromolecules - July 14, 2019 Category: Biochemistry Authors: Harada K, Nambu Y, Mizuno S, Tsuge T Tags: Int J Biol Macromol Source Type: research

Optimized immobilization of polygalacturonase from Aspergillus niger following different protocols: Improved stability and activity under drastic conditions.
Abstract Polygalacturonase (PG) from Aspergillus niger was immobilized using glyoxyl, vinylsulfone or glutaraldehyde-activated supports. The use of supports pre-activated with glutaraldehyde presented the best results. The immobilization of PG on glutaraldehyde-supports was studied under different conditions: at pH 5 for 24 h; at pH 5, 6.5 or 8 for 3 h and then incubated at pH 8 for 24 h; at pH 8 in the presence of 300 mM NaCl for 24 h, to prevent ion exchange. The immobilization under all conditions showed a significant increase in the enzyme thermal stability under inactivation conditions at pH...
Source: International Journal of Biological Macromolecules - July 14, 2019 Category: Biochemistry Authors: Magro LD, Kornecki JF, Klein MP, Rodrigues RC, Fernandez-Lafuente R Tags: Int J Biol Macromol Source Type: research

Design, development and validation of guar gum based pH sensitive drug delivery carrier via graft copolymerization reaction using microwave irradiations.
Abstract A natural polysaccharide exudate guar gum was modified via graft copolymerization reaction aided by microwave irradiation. The graft copolymerization was carried out by using 2-hydroxyl ethyl methacrylate (PHEMA) as monomer and ceric ammonium nitrate (CAN) as a free radical initiator under microwave irradiation. Different grades of graft copolymers of (GG-g-P(HEMA)) were designed by varying the initiator and monomer concentrations and the best grade was developed with maximum percentage of grafting. The validation of synthesis was done by different characterization techniques such as 13C NMR, FTIR, SEM, C...
Source: International Journal of Biological Macromolecules - July 13, 2019 Category: Biochemistry Authors: Mahto A, Mishra S Tags: Int J Biol Macromol Source Type: research

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) terpolymer production from volatile fatty acids using engineered Ralstonia eutropha.
In this study, we aimed to synthesize the terpolymer P(3HB-co-3HV-co-3HHx) from volatile fatty acids such as propionate and butyrate using the recombinant Ralstonia eutropha strain (Re2133/pCB81), containing deletions in the phaB1, phaB2, and phaB3 genes, and overexpression of synthetic PHA operon (phaC2, phaA, phaJ). This strain produced terpolymers depending on the ratio of two different carbon sources, namely, propionic and butyric acids; however, wild type R. eutropha could not produce the same type of polymer. The incorporation of 3-hydroxyvalerate and 3-hydroxyhexanoate monomers was confirmed by gas chromatography an...
Source: International Journal of Biological Macromolecules - July 13, 2019 Category: Biochemistry Authors: Jung HR, Jeon JM, Yi DH, Song HS, Yang SY, Choi TR, Bhatia SK, Yoon JJ, Kim YG, Brigham CJ, Yang YH Tags: Int J Biol Macromol Source Type: research

Expression and characterisation of feruloyl esterases from Lactobacillus fermentum JN248 and release of ferulic acid from wheat bran.
Abstract Genes encoding six feruloyl esterases (FAEs; lbff0997, lbff0272, lbff1432, lbff1695, lbff1849, lbff0153) from Lactobacillus fermentum JN248 were cloned, overexpressed and characterised. Maximum enzyme activity was observed at 35 °C for recombinant FAEs LFFae0997, LFFae0272 and LFFae0153, at 30 °C for LFFae1695, and at 40 °C for LFFae1432and LFFae1849. For five of the enzymes, optimal activity was observed at pH 7.0 or pH 8.0, and high thermostability was measured up to 55 °C. By contrast, LFFae1432 lost less than 10.0% activity after incubation at 40 °C for 2 h, and pH ...
Source: International Journal of Biological Macromolecules - July 12, 2019 Category: Biochemistry Authors: Deng H, Jia P, Jiang J, Bai Y, Fan TP, Zheng X, Cai Y Tags: Int J Biol Macromol Source Type: research

Evaluating the glycophenotype on breast cancer tissues with quantum dots-Cramoll lectin conjugates.
In conclusion, QDs-Cramoll conjugates can be considered effective, specific, and versatile probes able to be applied to evaluate glycan profiles in normal and transformed tissues, by fluorescence microscopy as well as FMA quantification. Furthermore, FMA showed to be a potential method that can be applied with other fluorescent conjugates. PMID: 31306700 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 12, 2019 Category: Biochemistry Authors: Carvalho MET, Oliveira WF, Cunha CRA, Coelho LCBB, Silva MV, Carvalho Junior LB, Santos BS, Cabral Filho PE, Fontes A, Correia MTS Tags: Int J Biol Macromol Source Type: research

Detection of a prostate cancer cell line using a bioluminescent affiprobe: An attempt to develop a new molecular probe for ex vivo studies.
In this study, we report on a novel bioluminescence affiprobe and evaluate its functionality by monitoring HER2 expression in the prostate cancer cells. The proposed bioluminescence affiprobe consists of a binding domain, an HER2 specific affibody molecule and a bioluminescent domain, recombinant Renilla luciferase. The experiments indicate that bioluminescence affiprobe can serve as a reliable and user-friendly probe for the detection of human HER2 positive prostate cells as well as ex vivo based detection of HER2-positive human prostate cancer specimens using luminometeric-based assays. PMID: 31306701 [PubMed - as s...
Source: International Journal of Biological Macromolecules - July 12, 2019 Category: Biochemistry Authors: Varnosfaderani Z, Emamzadeh R, Nazari M, Zarean M Tags: Int J Biol Macromol Source Type: research

Alkali induced unique partially folded state of bovine serum albumin: qualitative and quantitative insights.
Abstract Qualitative and quantitative understanding of partially folded states of protein is essential in gaining deeper insights into folding pathways. We have observed a partially folded state of bovine serum albumin (BSA) in the presence of 2,2,2-trifluoroethanol at pH 11.2 which does not resembles the properties of the molten globule state. ThT, a frequently used marker for protein fibrils have two order of greater affinity towards the intermediate state at pH 11.2 compared to native BSA at pH 7.4. Surprisingly, the binding of ANS with this partially folded state is weaker than that of native state of BS...
Source: International Journal of Biological Macromolecules - July 12, 2019 Category: Biochemistry Authors: Prasanthan P, Kishore N Tags: Int J Biol Macromol Source Type: research

Fabrication of stable zein nanoparticles by chondroitin sulfate deposition based on antisolvent precipitation method.
Abstract The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles. The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS. Zein/CS composite nanoparticles were fabricated by antisolvent precipitation method at pH 4.0. The optimal mass ratio of zein to CS was 1:1 (w/w). Zein/CS composite nanoparticles showed mean size of 148 nm and PDI 
Source: International Journal of Biological Macromolecules - July 12, 2019 Category: Biochemistry Authors: Yuan Y, Li H, Liu C, Zhu J, Xu Y, Zhang S, Fan M, Zhang D, Zhang Y, Zhang Z, Wang D Tags: Int J Biol Macromol Source Type: research

Impact of acid type for chitosan dissolution on the characteristics and biodegradability of cornstarch/chitosan based films.
Abstract Biopolymers have received considerable attention in recent years given the environmental concern related to the incorrect disposal of materials based on synthetic plastics. Starch and chitosan are good examples of raw materials of interest, and its combination can exhibit some newsworthy barrier and mechanical properties. Therefore, this study aimed to determine the impact of the acid type, acetic and lactic solutions (1% v/v), used for chitosan solubilization and the starch:chitosan proportion on the properties of cornstarch/chitosan-based films. The films were prepared by casting and characterized by an...
Source: International Journal of Biological Macromolecules - July 12, 2019 Category: Biochemistry Authors: Pavoni JMF, Luchese CL, Tessaro IC Tags: Int J Biol Macromol Source Type: research

The antiviral property of Sargassum fusiforme polysaccharide for avian leukosis virus subgroup J in vitro and in vivo.
This study offered a new method for ALV-J treatment and enriched the potential application of SFP. PMID: 31306705 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 12, 2019 Category: Biochemistry Authors: Sun Y, Chen X, Zhang L, Liu H, Liu S, Yu H, Wang X, Qin Y, Li P Tags: Int J Biol Macromol Source Type: research

Strong and thermally insulating polylactic acid/glass fiber composite foam fabricated by supercritical carbon dioxide foaming.
Abstract Environmental friendly and non-toxic polylactic acid (PLA) foam exhibits a promising perspective in many areas. However, PLA shows very poor foaming ability. Herein, silane-modified glass fiber (GF) was compounded with PLA to improve its foaming ability. Thanks to the increased melt viscoelasticity and melt strength, and the enhanced crystallization by adding GF, PLA/GF composites show dramatically improved foaming ability, characterized by widened processing window, increased expansion ratio, and improved cellular uniformity. Compared with pure PLA foam, PLA/GF composite foam shows dramatically enhanced ...
Source: International Journal of Biological Macromolecules - July 12, 2019 Category: Biochemistry Authors: Wang J, Chai J, Wang G, Zhao J, Zhang D, Li B, Zhao H, Zhao G Tags: Int J Biol Macromol Source Type: research

Nanobiocatalyst facilitated aglycosidic quercetin as a potent inhibitor of tau protein aggregation.
Abstract Polyphenols have been suggested as potential therapeutic agents for the treatment of amyloidogenic diseases. In this work, we evaluate quercetin-rich onion extract for its ability to inhibit tau fibrillization. Considering the presence of polyphenols in multiple glycosidic and aglycosidic forms, a nanobiocatalyst-mediated approach has been used to extract quercetin from onion skins. The nanobiocatalysts facilitate greater release of quercetin compared to the use of free enzymes. Atomic force microscopy and fluorescence microscopy show that quercetin possesses a novel inhibitory character on tau-fibril agg...
Source: International Journal of Biological Macromolecules - July 12, 2019 Category: Biochemistry Authors: Kumar S, Krishnakumar VG, Morya V, Gupta S, Datta B Tags: Int J Biol Macromol Source Type: research

Loading paclitaxel into porous starch in the form of nanoparticles to improve its dissolution and bioavailability.
Abstract In this work, paclitaxel was loaded into porous starch in the form of nanoparticles (PNPS), and the properties of PNPS were investigated by using raw paclitaxel and the system of paclitaxel directly loaded into porous starch (PPS) as control groups. According to the tested results, the drug loading (DL) and encapsulation efficiency (EE) of PNPS were 14.13% ± 0.27% and 73.92% ± 0.54%, higher than that of PPS (9.79% ± 0.31% and 71.17% ± 0.67%) respectively. Compared with raw paclitaxel and PPS, PNPS exhibited the more prominent dissolution rate and bioavailability...
Source: International Journal of Biological Macromolecules - July 12, 2019 Category: Biochemistry Authors: Wang L, Zhao X, Yang F, Wu W, Wu M, Li Y, Zhang X Tags: Int J Biol Macromol Source Type: research

Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function.
In this study, we employed multiple spectroscopic methods to analyze the effects of carbon nanoparticles (CNPs) on structure of cytochrome c (Cyt c) and mitochondrial function in plant cells. The tertiary structures of aromatic amino acid in Cyt c were not changed after addition of CNPs. Cyt c was found to be absorbed on the surfaces of CNPs in a non-linear manner and only bound Cyt c can be reduced. In addition, the binding of Cyt c was found to increase the diameter of CNPs at lower concentrations. The redox potential of Cyt c was almost not affected after treatment with CNPs. There were no obvious differences in cellula...
Source: International Journal of Biological Macromolecules - July 11, 2019 Category: Biochemistry Authors: Chen L, Hao J, Xu L, Meng X, Li X, Nie C, Xie F, Liu K, Peng X, Xie J, Liang T, Guo Z Tags: Int J Biol Macromol Source Type: research

Molecular recognition based rapid diagnosis of immunoglobulins via proteomic profiling of protein-nanoparticle complexes.
Abstract Protein-nanoparticle (NP) interaction, which inevitably form protein corona (PC), has been the subject of much debate about its role in modern biomedical research. In this regard, PC associated with two different NPs viz., magneto-fluorescent (MF) and chitosan coated MF (CMF) NPs were thoroughly investigated, to analyze the effect of polymer coating on protein adsorption. Bradford assay, along with the spectroscopic and microscopic studies suggested increase in adsorbed protein quantity, though the results varied significantly on moving from bare to polymeric coating and in vitro to ex vivo conditions. In...
Source: International Journal of Biological Macromolecules - July 11, 2019 Category: Biochemistry Authors: Dar AI, Walia S, Acharya A Tags: Int J Biol Macromol Source Type: research

Osteogenic differentiation of hMSCs on semi-interpenetrating polymer networks of polyurethane/poly(2 ‑hydroxyethyl methacrylate)/cellulose nanowhisker scaffolds.
Osteogenic differentiation of hMSCs on semi-interpenetrating polymer networks of polyurethane/poly(2‑hydroxyethyl methacrylate)/cellulose nanowhisker scaffolds. Int J Biol Macromol. 2019 Jul 11;: Authors: Shahrousvand M, Ghollasi M, Zarchi AAK, Salimi A Abstract Poly (2‑hydroxyethyl methacrylate) (PHEMA) was crosslinked in the presence of biocompatible and biodegradable poly(caprolactone) (PCL) based polyurethanes (PUs) and cellulose nanowhiskers (CNWs). The CNWs were obtained from wastepaper. In order to crosslink PHEMA (10 wt%), a novel acrylic-urethane cross-linker was produced by a condensat...
Source: International Journal of Biological Macromolecules - July 11, 2019 Category: Biochemistry Authors: Shahrousvand M, Ghollasi M, Zarchi AAK, Salimi A Tags: Int J Biol Macromol Source Type: research

Development and physicochemical, thermal and mechanical properties of edible films based on pumpkin, lentil and quinoa starches.
This study demonstrated that starches from non-conventional sources are good alternative to conventional to develop edible films. PMID: 31302126 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 11, 2019 Category: Biochemistry Authors: Pająk P, Przetaczek-Rożnowska I, Juszczak L Tags: Int J Biol Macromol Source Type: research

Enhanced decolorization of malachite green by a magnetic graphene oxide-CotA laccase composite.
In this study, a CotA laccase from Bacillus subtilis cjp3 was successfully immobilized onto magnetic graphene oxide (MGO) nanomaterials via covalent bonding with hydrochloride/N-hydroxysuccinimide (EDC/NHS). The morphology, structure, and properties of the MGO-laccase were then characterized by scanning-electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), X-ray-photoelectron spectroscopy (XPS), and a magnetic-property-measurement system (MPMS). The magnetic composite exhibited an extremely high binding capacity of ~14...
Source: International Journal of Biological Macromolecules - July 11, 2019 Category: Biochemistry Authors: Qiao W, Liu H Tags: Int J Biol Macromol Source Type: research

Optimization of induction parameters, structure quality assessment by ATR-FTIR and in silico characterization of expressed recombinant polcalcin in three different strains of Escherichia coli.
This study was conducted to characterize biophysical differences of recombinant polcalcin come from three different Escherichia coli strains using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), optimize recombinant polcalcin expression, and linear B-cell epitopes identification using in silico methods. ATR-FTIR results of purified proteins showed spectra intensity variance in the amide I region, while there were no changes in pick position and shape of the bands. SHuffle® T7 Express was selected for subsequent optimization due to ability in the correction of the mis-oxidized bonds and ...
Source: International Journal of Biological Macromolecules - July 11, 2019 Category: Biochemistry Authors: Derakhshani A, F KK, Banadkoki SB, Shirazi FH, Barati M, Fereidouni M, Safarpour H Tags: Int J Biol Macromol Source Type: research

A redox cycle meets insulin fibrillation in vitro.
We describes here an unexpected finding that resazurin plays a role in modulating insulin fibrillation, whereas resorufin doesn't. We hypothesize that the positively charged insulin at low pH can combine with the negatively charged resazurin due to electrostatic interaction, through which resazurin inhibited the process of insulin fibrillation. This effect was characterized and verified by using various biochemical, spectroscopic and imaging tools. This inhibition of this biocompatible dye can be achieved at various stages of fibrillation, suggesting the toxicity of the protein fibrils can be eliminated by using resazurin....
Source: International Journal of Biological Macromolecules - July 11, 2019 Category: Biochemistry Authors: Wang H, Ding H, Ma B, Chen Z Tags: Int J Biol Macromol Source Type: research

Botryosphaeran and sulfonated derivatives as novel antiviral agents for herpes simplex and dengue fever.
This study demonstrated for the first time that native botryosphaeran inhibited HSV infection, albeit moderately, while its sulfonated derivatives developed high activity against viral infection. DENV inhibition was weak for botryosphaeran, but remarkably stronger for S1 and S2. All compounds were inactive against PV, as it lacked a viral envelope. The presence of sulfate groups and the DS were confirmed to be important features for antiviral activity. PMID: 31302130 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 11, 2019 Category: Biochemistry Authors: Sacchelli BAL, Faccin-Galhardi LC, Ito VY, Lopes JL, Dekker RFH, Barbosa-Dekker AM, Orsato A Tags: Int J Biol Macromol Source Type: research

Effective adsorption of heavy metal ions by sodium lignosulfonate reformed montmorillonite.
Abstract Sodium lignosulfonate (Na-LS) was used to modified montmorillonite to form lignosulfonate-montmorillonite (LS-MMT). The as-prepared samples were characterized by various equipment to analyze the surface morphology and structure of the pure and reformed samples. The adsorption capability (qe, mg/g) of LS-MMT or Ca/Na-MMT for Pb(II) and Cu(II) at different reaction intervals, the concentration of metal ions, temperature, and pH were studied by batch adsorption experiments. The results revealed that LS-MMT displayed a higher adsorption capacity than Ca/Na-MMT. The pH of solutions had a great influence on the...
Source: International Journal of Biological Macromolecules - July 11, 2019 Category: Biochemistry Authors: Ma J, Khan MA, Xia M, Fu C, Zhu S, Chu Y, Lei W, Wang F Tags: Int J Biol Macromol Source Type: research

Morphological, thermal, rheological and mechanical properties of poly (butylene carbonate) reinforced by stereocomplex polylactide.
This report may open a new avenue to achieve higher-performance biodegradable polymer blend materials. PMID: 31301391 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 10, 2019 Category: Biochemistry Authors: Li Y, Han C, Yu Y, Huang D Tags: Int J Biol Macromol Source Type: research

Surface-modified nanocrystalline cellulose from oil palm empty fruit bunch for effective binding of curcumin.
Abstract Rod-shape particles appeared to have long circulatory time, tumor accumulation and high cellular uptake as drug carrier. Acid hydrolysed nanocrystalline cellulose (NCC) from empty fruit bunch exhibits a width of 13-30 nm and length of 150-360 nm in rod-shape structure, holds good potential for high level of drug loading. Moreover, NCC is particularly superior as a favorable bio-based carrier owing to its biodegradability and biocompatibility. The Fourier-transform infrared spectroscopy results confirmed the binding of curcumin on the modified NCC with notable spectra changes caused by tannic acid (TA)...
Source: International Journal of Biological Macromolecules - July 10, 2019 Category: Biochemistry Authors: Foo M, Tan C, Lim P, Ooi EC, Tan K, Chew IM Tags: Int J Biol Macromol Source Type: research

Green magnetic hydrogels synthesis, characterization and flavourzyme immobilization based on chitin from Hericium erinaceus residue and polyvinyl alcohol.
In this study, a novel magnetic sensitive hydrogel was prepared based on fungal chitin extracted from Hericium erinaceus residue and polyvinyl alcohol (PVA) through a green and facile procedure of repeated freezing-thaw and in situ decoration of Fe3O4. The prepared hydrogels were characterized and applied for flavourzyme immobilization. The results showed that magnetic Fe3O4 was successfully introduced into chitin/PVA matrix due to the porous networks of the hydrogels, and the prepared hydrogels displayed superparamagnetic behavior and improved gel strength due to the presence of Fe3O4. The efficiency for flavourzyme immob...
Source: International Journal of Biological Macromolecules - July 10, 2019 Category: Biochemistry Authors: Liao J, Huang H Tags: Int J Biol Macromol Source Type: research

Heterologous expression of Stlac2, a laccase isozyme of Setosphearia turcica, and the ability of decolorization of malachite green.
CONCLUSIONS: Generally, recombinant protein of fungal laccase Stlac2 was active without glycosylation and decolorize malachite green efficiently, which has potential industrial applications. PMID: 31301394 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - July 10, 2019 Category: Biochemistry Authors: Liu N, Shen S, Jia H, Yang B, Guo X, Si H, Cao Z, Dong J Tags: Int J Biol Macromol Source Type: research