Understanding the digestibility of rice starch-gallic acid complexes formed by high pressure homogenization.
Abstract Rice starch-Gallic acid (GA) complex (RSP-GA) was prepared by high pressure homogenization (HPH), and the effect of GA on the digestibility and multi-structure of rice starch under HPH was investigated. The results showed that, after HPH, the digestibility of starch substantially changed in the reduced rapidly digestible starch (RDS) content, and increased resistant starch (RS) after interacting with GA. In particular, the RS content of RSP-GA ranged from 5.4% to 29.7%, which were much higher than that of rice starch (1.6%). Meanwhile, the results indicated that rice starch and GA were aggregated by hydro...
Source: International Journal of Biological Macromolecules - May 16, 2019 Category: Biochemistry Authors: Liu Y, Chen L, Xu H, Liang Y, Zheng B Tags: Int J Biol Macromol Source Type: research

Lignin and its blends.
PMID: 31103594 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 16, 2019 Category: Biochemistry Authors: Collins MN Tags: Int J Biol Macromol Source Type: research

An adsorbent based on humic acid and carboxymethyl cellulose for efficient dye removal from aqueous solution.
In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH). The as-obtained adsorbent, denoted as HA-CMC/PMH (PCH and PFH were referred to as PMH), was analyzed by infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and thermo gravimetric analysis. When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH. The adsorption mechanisms of MB involved ion ex...
Source: International Journal of Biological Macromolecules - May 16, 2019 Category: Biochemistry Authors: Lu S, Liu W, Wang Y, Zhang Y, Li P, Jiang D, Fang C, Li Y Tags: Int J Biol Macromol Source Type: research

Modified nano microfibrillated cellulose/carboxymethyl chitosan composite hydrogel with giant network structure and quick gelation formability.
Abstract Carboxymethyl chitosan (CMCS) has good biocompatibility, biodegradability and water solubility. This work investigated a nanocellulose fibrils reinforced CMCS composite hydrogel with giant network structure and quick gelation formability. The microfibrillated cellulose (MFC) with diameter in nano scale and length in sub-micron scale was obtained through high-pressure homogenization. The hydroxyl groups on the MFC nanofibrils surface were partly oxidized into aldehyde groups. The dialdehyde MFC (DAMFC), remaining the fibril morphology and fine three-dimensional network structure, was mixed with CMCS. Due t...
Source: International Journal of Biological Macromolecules - May 15, 2019 Category: Biochemistry Authors: Wei D, Liu Q, Liu Z, Liu J, Zheng X, Pei Y, Tang K Tags: Int J Biol Macromol Source Type: research

Incorporation of collagen and PLGA in bioactive glass: in vivo biological evaluation.
; ACM Abstract Bioactive glasses (BG) are known for their unique ability to bond to bone tissue. However, in critical situations, even the osteogenic properties of BG may be not sufficient to produce bone consolidation. The use of composite materials may constitute an optimized therapeutical intervention for bone stimulation. The aim of this study was to characterize BG/collagen/poly (d,l-lactic-co-glycolic) acid (BG/COL/PLGA) composites, in vitro biocompatibility and in vivo biological properties. MC3T3-E1 cells were evaluated by cell proliferation, ALP activity, cell adhesion and morphology. Qualitative histolog...
Source: International Journal of Biological Macromolecules - May 15, 2019 Category: Biochemistry Authors: Magri AMP, Fernandes KR, Assis L, Kido HW, Avanzi IR, da Cruz Medeiros M, Granito RN, Braga FJC, Rennó ACM Tags: Int J Biol Macromol Source Type: research

In-situ fast gelling formulation for oral sustained drug delivery of paracetamol to dysphagic patients.
Abstract Evaluation of the potential for oral sustained drug delivery of formulations with in-situ gelling properties is the main objective of the present investigation. Oral administration of aqueous dispersion of sodium alginate (1.5% w/v) containing calcium ions in complex form resulted in the formation of gel matrix as a consequence of the release of the calcium ions in the acidic environment of stomach fluid. Addition of methylcellulose, sodium chloride and polyethylene glycol improved the drug retention efficacy of the gel. In this investigation, the study on the influence of added excipients on the rheologi...
Source: International Journal of Biological Macromolecules - May 15, 2019 Category: Biochemistry Authors: Sharma S, Sarkar G, Srestha B, Chattopadhyay D, Bhowmik M Tags: Int J Biol Macromol Source Type: research

Exploring structural aspects of the human Golgi matrix protein GRASP55 in solution.
Abstract In mammals, the Golgi apparatus is the central hub for intracellular trafficking, sorting and post-translational modifications of proteins and lipids. Golgi reassembly and stacking proteins (GRASPs) are somehow involved in Golgi stacking, which is relevant for its proper function, and also in unconventional protein secretion. However, the structural details on how GRASPs accomplish those tasks are still elusive. Here, we have explored the biochemical and biophysical properties of human full-length GRASP55 in solution. Sequence-based analyses and circular dichroism spectroscopy suggest that GRASP55 present...
Source: International Journal of Biological Macromolecules - May 15, 2019 Category: Biochemistry Authors: Reddy ST, Mendes LFS, Fontana NA, Costa-Filho AJ Tags: Int J Biol Macromol Source Type: research

Structural and in vitro digestibility properties of esterified maca starch with citric acid and its application as an oil-in-water (O/W) pickering emulsion stabilizer.
The objective of the present study was to determine structural and in-vitro digestibility properties of esterified maca starch with citric acid (EMSC) and explore its application in oil-in-water (O/W) pickering emulsion. EMSC was prepared by esterifying native maca starch (NMS) with different concentrations of citric acid (0, 10, 20, and 30%, w/w). The structural properties were characterized by SEM, FT-IR, XRD, and 13C and 1H NMR spectroscopy, which demonstrated that EMSC exhibited new ester linkages from citric acid in NMS. The in vitro digestibility results showed that EMSC had the significantly lower rapidly digestible...
Source: International Journal of Biological Macromolecules - May 15, 2019 Category: Biochemistry Authors: Lee YK, Chang YH Tags: Int J Biol Macromol Source Type: research

Calcium ion-exchange cross-linked porous starch microparticles with improved hemostatic properties.
Abstract Starch hemostatic agents have been clinically used in surgical hemostasis in recent years. Calcium ion (Ca2+)-exchange cross-linked porous starch microparticles (Ca2+CPSMs) were prepared as a new hemostatic agent to enhance the hemostatic efficacy. A series of Ca2+CPSMs with varying Ca2+ contents were prepared and characterized by Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), scanning electron microscope (SEM), and ion content analysis. The XPS and FT-IR results indicated that the surface of Ca2+CPSMs was modified by Ca2+, which might for...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Zhu J, Sun Y, Sun W, Meng Z, Shi Q, Zhu X, Gan H, Gu R, Wu Z, Dou G Tags: Int J Biol Macromol Source Type: research

Characterization and comparative studies of galactomannans from Bauhinia vahlii, Delonix elata, and Peltophorum pterocarpum.
Abstract The present investigation determines the extraction and characterization of seed galactomannans from the Leguminosae taxa of Bauhinia vahlii, Delonix elata, and Peltophorum pterocarpum. The seed galactomannans presented a total yield of 30.82, 24.01 and 25.25% with a Man/Gal ratios of 4.21:1, 2.55:1 and 3.03:1 for B. vahlii, D. elata, and P. pterocarpum, respectively exhibiting 1-4 mannose linkages with galactose side chains at C6 position. The galactomannans presented an efficient water holding capacity, solubility and emulsion properties. Intrinsic viscosity from combined Huggins and Kraemer extrapolati...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Jamir K, Badithi N, Venumadhav K, Seshagirirao K Tags: Int J Biol Macromol Source Type: research

Covalent immobilization and characterization of penicillin G acylase on amino and GO functionalized magnetic Ni0.5Zn0.5Fe2O4@SiO2 nanocomposite prepared via a novel rapid-combustion process.
Abstract Magnetic Ni0.5Zn0.5Fe2O4@SiO2 nanocomposite was prepared via the rapid combustion process, and its surface was modified to obtain amino-functionalized magnetic Ni0.5Zn0.5Fe2O4@SiO2-NH2 nanocomposite. The modified nanocomposite was loaded on graphene oxide (GO), on which penicillin G acylase (PGA) was covalently immobilized. The structure for docking was visualized between PGA and penicillin G using the PyMol program, which revealed the configuration of the active site. Selections of immobilization conditions including immobilization concentration and time of fixation, were explored. The catalytic performa...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Yu Q, Wang Z, Zhang Y, Liu R Tags: Int J Biol Macromol Source Type: research

Thermo and alkali stable β-mannanase: Characterization and application for removal of food (mannans based) stain.
Thermo and alkali stable β-mannanase: Characterization and application for removal of food (mannans based) stain. Int J Biol Macromol. 2019 May 14;: Authors: Singh S, Singh G, Khatri M, Kaur A, Arya SK Abstract The present work aims to characterize thermo and alkali stable β-mannanase (ManSS11) from newly isolated Klebsiella pneumoniae SS11 and its food stain (mannan based) removal efficiency. The enzyme, ManSS11 was stable over a wide range of pH and temperature. The highest activity of ManSS11 was observed at pH 9.0 and temperature, 70 °C, with t1/2 of 135.91 min at the same temp...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Singh S, Singh G, Khatri M, Kaur A, Arya SK Tags: Int J Biol Macromol Source Type: research

Gelation of hydroxyethyl cellulose aqueous solution induced by addition of colloidal silica nanoparticles.
In this study, a series of hydrogels based on the physical adsorption between hydroxyethyl cellulose (HEC) and colloidal silica nanoparticles (SNPs) were developed, and the effects of SNPs addition on the prepared gels were also investigated. The addition of SNPs into the HEC solution (1.0 wt%) induced gelation, and the gel strength was enhanced by increasing the SNPs concentration from 5 to 20 wt%, which was due to the formation of a denser gel network, as evidenced by SEM observations. Rheological tests suggested that the gelation temperature (Tg) was decreased with the increasing SNPs addition, and the gels exhibite...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Gong T, Hou Y, Yang X, Guo Y Tags: Int J Biol Macromol Source Type: research

The fucoidan from the seaweed Ascophyllum nodosum enhances RCT-related genes expression in hyperlipidemic C57BL/6J mice.
In conclusion, this fucoidan lowers lipid by enhancing RCT-related genes expression, and it can be explored as a potential candidate for prevention or treatment of lipid disorders. PMID: 31100394 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Yang Z, Yin J, Wang Y, Wang J, Xia B, Li T, Yang X, Hu S, Ji C, Guo S Tags: Int J Biol Macromol Source Type: research

Development of bioactive composite films from chitosan and carboxymethyl cellulose using glutaraldehyde, cinnamon essential oil and oleic acid.
Abstract In the present research the effect of incorporating cinnamon essential oil (CEO), oleic acid (OA) and glutaraldehyde (GL) on antimicrobial, antioxidant, mechanical, physical and morphological properties of chitosan-carboxymethyl cellulose composite films were investigated. Cross-linking effect of GL (as determined by FTIR analysis) significantly resulted in higher mechanical strength and lower water vapor permeability (WVP). Cross-linked films incorporated with CEO revealed higher and steady state antioxidant activity as well as in vitro antimicrobial properties against Listeria monocytogenes and Pseudomo...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Valizadeh S, Naseri M, Babaei S, Hosseini SMH, Imani A Tags: Int J Biol Macromol Source Type: research

Effect of ethanol content on rheology of film-forming solutions and properties of zein/chitosan film.
Abstract The paper focused on the role of ethanol on rheological properties of zein/chitosan film-forming solutions, thermo-mechanical and structural characterization of films. The steady rheological results indicated that the film-forming solutions showed non-Newtonian behavior, and ZC-3 solution (zein treated with 90% ethanol and chitosan dissolved with acetic acid) had higher flow index (0.882 ± 0.003). Based on dynamic rheological analysis, ZC-3 solution showed the highest G' value and the crossover point at the lowest frequency, representing the strongest entanglement and hydrogen bonding. ZC-3 fil...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Zhang L, Liu Z, Han X, Sun Y, Wang X Tags: Int J Biol Macromol Source Type: research

Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.
In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs). The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs). The maximum ASE-HDPs yield was obtained using a response surface method (RSM) with optimized ASE conditions comprising 2 cycles at 130 °C for 23 min. The maximum ASE-HDPs yield of 8.62 ± 0.29% is significantly higher than the maximum HWE-HDP yield of 6.89 ± 0.21...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Yang B, Wu Q, Luo Y, Yang Q, Chen G, Wei X, Kan J Tags: Int J Biol Macromol Source Type: research

Structural characterization and immunostimulatory activity of a novel polysaccharide from green alga Caulerpa racemosa var peltata.
Abstract A novel water-soluble polysaccharide (named CRVP-1) was successfully obtained from Caulerpa racemosa var peltata by hot-water extraction, ethanol precipitation, and column chromatography. The structure of CRVP-1 was characterized by HPGPC, HPAEC-PAD, FT-IR, GC-MS and NMR. The structural analysis indicated that CRVP-1 possessed a sulfate content of 25.8% ± 0.7% and was a heteropolysaccharide with an average molecular weight of 29.68 kDa, and composed of mannose, galactose, glucose, galacturonic acid, and glucuronic acid with rates of 92.1%, 2.9%, 1.8%, 1.7% and 1.2%, respectively, owning a bac...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Hao H, Han Y, Yang L, Hu L, Duan X, Yang X, Huang R Tags: Int J Biol Macromol Source Type: research

Dissolution of less-processed wood fibers without bleaching in an ionic liquid: Effect of lignin condensation on wood component dissolution.
This study evaluated the solubility of low-processed wood fibers produced from a low temperature acid hydrotropic fractionation (AHF) in an ionic liquid (IL) 1,5-diazabicyclo[4.3.0]non-5-enium acetate (DBNH[OAc]. Unbleached wood fibers produced from AHF lignin fractionation using p-Toluenesulfonic acid (p-TsOH) with or without post-treatments with lignin and hemicelluloses content up to 15% and 25%, respectively, were dissolved directly into the IL DBNH[OAc]. Post-AHF fractionation treatments using dilute alkaline and glycerol swelled the cellulose and partially removed residual lignin resulting in improved dissolution. Se...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Wang H, Hirth K, Zhu J, Ma Q, Liu C, Zhu JY Tags: Int J Biol Macromol Source Type: research

Biochemical characterization and mutational studies of the thermostable uracil DNA glycosylase from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5.
Abstract Uracil DNA glycosylases (UDGs) play an important role in removing uracil from DNA to initiate DNA base excision repair. Here, we characterized biochemically a thermostable UDG from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5 (Tba UDG), and probed its mechanism by mutational analysis. The recombinant Tba UDG cleaves exclusively uracil-containing ssDNA and dsDNA at 65 °C. The enzyme displays an optimal cleavage activity at 70-75 °C. Tba UDG cleaves DNA over a wide pH spectrum ranging from 4.0 to 11.0 with an optimal pH of 7.0-9.0. In addition, Tba UDG activity is independent o...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Shi H, Gan Q, Jiang D, Wu Y, Yin Y, Hou H, Chen H, Xu Y, Miao L, Yang Z, Oger P, Zhang L Tags: Int J Biol Macromol Source Type: research

Role of Herborn (K240E) and Milano Slow (D375H) human serum albumin variants towards binding of phenylbutazone and ibuprofen.
Abstract Human serum albumin (HSA) is the binding cargo in blood plasma. The binding of drugs to HSA determines the pharmacokinetics and pharmacodynamics of the drugs. There are 67 natural genetic variants of HSA were reported in literature. Studying the effect of albumin modifications on drug binding helps to treat the patients with proper medication. In the present study, we have aimed to understand the effect of two natural variants of HSA, such as Herborn (K240E) and Milano Slow (D375H) on the binding of phenylbutazone and ibuprofen. For this, we have generated K240E and D375H mutants and also double mutant (K...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Nerusu A, Chinthapalli DK, Subramanyam R Tags: Int J Biol Macromol Source Type: research

Antimicrobial and swelling behaviors of novel biodegradable corn starch grafted/poly(4-acrylamidobenzoic acid) co-polymers.
Abstract Novel antimicrobial copolymers resulted from free radical copolymerization of gelatinized corn starch in aqueous solution with different amounts of 4-acrylamidobenzoic acid monomer using ammonium persulfate (APS)/sodium bisulfite (NaHSO3) as a redox initiator. The grafting copolymerization was evidenced by FTIR, 1HNMR, scanning electron microscopy (SEM) and X-ray diffraction. The thermal stability was improved after the grafting reaction as detected from IPDT values calculated from the thermogravimetric analysis. The effect of changing grafting parameters (initiator concentration, monomer concentration, r...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Abd El-Ghany NA, Abdel Aziz MS, Abdel-Aziz MM, Mahmoud Z Tags: Int J Biol Macromol Source Type: research

Cantharellus cibarius branched mannans inhibits colon cancer cells growth by interfering with signals transduction in NF- ĸB pathway.
Cantharellus cibarius branched mannans inhibits colon cancer cells growth by interfering with signals transduction in NF-ĸB pathway. Int J Biol Macromol. 2019 May 14;: Authors: Lemieszek MK, Nunes FM, Marques G, Rzeski W Abstract In order to evaluate the therapeutic potential of polysaccharides obtained from the widely consumed Cantharellus cibarius mushroom on NF-ĸB pathway involved in cancer cells proliferation, survival, and metastasis, their antiproliferative and cytotoxic properties in human colon cancer cells LS180 and human colon epithelial cells CCD841 CoN were studied. BrdU and LDH assays r...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Lemieszek MK, Nunes FM, Marques G, Rzeski W Tags: Int J Biol Macromol Source Type: research

Three-dimensional gelatin/PVA scaffold with nanofibrillated collagen surface for applications in hard-tissue regeneration.
In this study, the surface of gelatin/PVA scaffold (CF-G5P5) coated with fibrillated collagen was fabricated to enhance cell proliferation and osteogenic differentiation for bone tissue regeneration. The physical and biological properties of the fabricated scaffolds were investigated. As a result, the CF-G5P5 scaffold increased surface roughness and increased protein absorption compared to a gelatin/PVA scaffold (G5P5) by 1.6 times from OD value 0.43 to 0.71 after 12 h, cell proliferation increased 1.7 times from OD value 0.57 to 0.96, and differentiation increased by 1.5 times from 100 to 151%. Based on the results, the...
Source: International Journal of Biological Macromolecules - May 14, 2019 Category: Biochemistry Authors: Kim H, Yang GH, Kim G Tags: Int J Biol Macromol Source Type: research

DNA/RNA recognition controlled by the glycine linker and the guanidine moiety of phenanthridine peptides.
ojković MR Abstract The binding of four phenanthridine-guanidine peptides to DNA/RNA was evaluated via spectrophotometric/microcalorimetric methods and computations. The minor structural modifications-the type of the guanidine group (pyrrole guanidine (GCP) and arginine) and the linker length (presence or absence of glycine)-greatly affected the conformation of compounds and consequently the binding to double- (ds-) and single-stranded (ss-) polynucleotides. GCP peptide with shorter linker was able to distinguish between RNA (A-helix) and DNA (B-helix) by different circular dichroism response at 295 nm and thus...
Source: International Journal of Biological Macromolecules - May 10, 2019 Category: Biochemistry Authors: Matić J, Šupljika F, Tandarić T, Dukši M, Piotrowski P, Vianello R, Brozovic A, Piantanida I, Schmuck C, Stojković MR Tags: Int J Biol Macromol Source Type: research

Stimulation of ATF3 interaction with Smad4 via TGF- β1 for matrix metalloproteinase 13 gene activation in human breast cancer cells.
Stimulation of ATF3 interaction with Smad4 via TGF-β1 for matrix metalloproteinase 13 gene activation in human breast cancer cells. Int J Biol Macromol. 2019 May 10;: Authors: Rohini M, Arumugam B, Vairamani M, Selvamurugan N Abstract We previously reported that transforming growth factor-β1 (TGF-β1) stimulated the sustained and prolonged expression of activating transcription factor 3 (ATF3) in highly metastatic and invasive human breast cancer cells (MDA-MB231), in contrast to normal human mammary epithelial cells. However, the mechanism behind the stability of ATF3 expression is not ...
Source: International Journal of Biological Macromolecules - May 10, 2019 Category: Biochemistry Authors: Rohini M, Arumugam B, Vairamani M, Selvamurugan N Tags: Int J Biol Macromol Source Type: research

Functionalized cellulose with hydroxyethyl methacrylate and glycidyl methacrylate for metal ions and dye adsorption applications.
Abstract Biodegradable functionalized cellulose based graft copolymers, Cell-g-HEMA and Cell-g-HEMA-co-GMA have been synthesized through free radical polymerization. The graft copolymers are used as sorbents for the removal of Ni(II), Cu(II) and Pb(II) ions, cationic (malachite green, crystal violet) and anionic (congo red) dyes as a function of adsorption time, temperature, pH of medium and concentration of metal ions and dyes. The sorption capacities of polymeric adsorbents for all the adsorbates decrease in the order Cell-g-HEMA-co-GMA > Cell-g-HEMA> Pure cellulose. Cell-g-HEMA-co-GMA removes 81.4%, 7...
Source: International Journal of Biological Macromolecules - May 10, 2019 Category: Biochemistry Authors: Sharma RK, Kumar R Tags: Int J Biol Macromol Source Type: research

Development of a reliable microRNA based electrochemical genosensor for monitoring of miR-146a, as key regulatory agent of neurodegenerative disease.
Abstract A MicroRNA (miR) based electrochemical method for quantification of miR-146a, a known biomarker for neurodegenerative disease, was developed. In this bioassay, the capture microRNA (C-miR) was self-assembled on the gold surface and used for quantification of target microRNA (T-miR) of miR-146a. For this purpose, an optimized concentration of C-miR was immobilized on the surface of gold electrode and used for capture of target analyte (T-miR). All of preparation steps were characterized by electrochemical techniques (cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)) and atomic force...
Source: International Journal of Biological Macromolecules - May 10, 2019 Category: Biochemistry Authors: Khalilzadeh B, Rashidi M, Soleimanian A, Tajalli H, Kanberoglu GS, Baradaran B, Rashidi MR Tags: Int J Biol Macromol Source Type: research

The retrogradation characteristics of pullulanase debranched field pea starch: Effects of storage time and temperature.
Abstract The structural changes and retrogradation behavior of the processed pea starch stored at 4 °C and 25 °C for different length of time (6, 12, 24, 48, and 72 h) was investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD), solid-state 13C nuclear magnetic resonance spectroscopy (13CNMR), Fourier transform infrared spectroscopy (FT-IR), and small angle X-ray scattering (SAXS), their corresponded physicochemical properties were studied by rapid visco-analyzer (RVA). A mixture of B- and V-type crystalline polymorph was observed by XRD for all processed and retrograded pea starc...
Source: International Journal of Biological Macromolecules - May 10, 2019 Category: Biochemistry Authors: Ma Z, Ma M, Zhou D, Li X, Hu X Tags: Int J Biol Macromol Source Type: research

Biosynthesis of β-d-glucan‑gold nanoparticles, cytotoxicity and oxidative stress in mouse splenocytes.
This study reports biosynthesis of gold-nanoparticles (AuNPs) by using β-d-glucans isolated from the yeast Yarrowia lypolitica D1. β-d-glucans serve as reducing and stabilizing mediators that induce the formation of AuNPs on the outer surface of the own β-d-glucan. The systems were physicochemically characterized by ultraviolet visible (UV-Vis) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), and dynamic light scattering (DLS) analyses. The results revealed the generation of AuNPs with quasi-spherical shap...
Source: International Journal of Biological Macromolecules - May 10, 2019 Category: Biochemistry Authors: Hernández-Adame L, Angulo C, Delgado K, Schiavone M, Castex M, Palestino G, Betancourt-Mendiola L, Reyes-Becerril M Tags: Int J Biol Macromol Source Type: research

A novel metalloprotease from banana peel and its biochemical characterization.
Abstract Protein cleaving enzymes, called proteases are one of the most commercially used enzymes possessing extensive applications in various industries. The present study was focused on screening, extraction, purification and characterization of protease from banana peel. Twelve different varieties of banana peels were screened for the protease activity. The maximum activity of the isolated enzyme was 230.4 CDU/mg from Nendran banana. The crude enzyme was purified up to 8.1 fold with the yield of 61.34%. The molecular weight of the purified fraction was found to be 40 kDa. The optimum conditions for maximum pr...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Gurumallesh P, Ramakrishnan B, Dhurai B Tags: Int J Biol Macromol Source Type: research

Tuned cell attachments by double-network hydrogels consisting of glycol chitosan, carboxylmethyl cellulose and agar bearing robust and self-healing properties.
Abstract Herein we present a tuned cell attachment on self-healable double network hydrogel bearing dynamic covalent bonds and hydrogen bonds. Agar formed first network, while glycol chitosan and oxidized carboxylmethyl cellulose formed second network in the resultant double network hydrogel. Because of the simple one-pot preparation, the hydrogel can be injected by using syringes. The moduli of the hydrogel were improved compared to that of the parent single-network. The hydrogel exhibited self-healing ability without need for heating or cut surface treatment. The incorporation of agar in the double network induc...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Cho IS, Ooya T Tags: Int J Biol Macromol Source Type: research

Polysaccharides from lichen Xanthoria parietina: 1,4/1,6- α-d-glucans and a highly branched galactomannan with macrophage stimulating activity via Dectin-2 activation.
Polysaccharides from lichen Xanthoria parietina: 1,4/1,6-α-d-glucans and a highly branched galactomannan with macrophage stimulating activity via Dectin-2 activation. Int J Biol Macromol. 2019 May 09;: Authors: Rashid ZM, Mormann M, Steckhan K, Peters A, Esch S, Hensel A Abstract Hot-water soluble polysaccharides H-1-3 and H-2-1 were isolated from the thalli of the lichen Xanthoria parietina (L.) Th. Fr. and purified by ion exchange and gel permeation chromatography. Structure elucidation was mainly based on 2D-NMR and nano-ESI-Q-TOF MS/MS experiments. H-1-3 (13.7 kDa) was shown to be linear &...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Rashid ZM, Mormann M, Steckhan K, Peters A, Esch S, Hensel A Tags: Int J Biol Macromol Source Type: research

Genomic dissection and transcriptional profiling of Cysteine-rich receptor-like kinases in five cereals and functional characterization of TaCRK68-A.
Abstract Cysteine-rich receptor-like kinases (CRK) constitute one of the largest subfamily of receptor-like kinases, which play crucial roles in plant development and stress response. In total, 43, 37, 36, 38 and 170 CRK genes including duplicated genes were identified in the genome of Brachypodium distachyon, Hordeum vulgare, Oryza sativa, Sorghum bicolor and Triticum aestivum, respectively. These CRK proteins were tightly clustered into four phylogenetic groups and exhibited close syntenic relationship among orthologous genes. Majority of CRK proteins contain a transmembrane domain for plasma membrane localizati...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Shumayla, Tyagi S, Sharma A, Singh K, Upadhyay SK Tags: Int J Biol Macromol Source Type: research

Purification of lysozyme from chicken egg white using nanofiber membrane immobilized with Reactive Orange 4 dye.
In this study, a dye-affinity nanofiber membrane (P-Chitosan-Dye membrane) was prepared by using polyacrylonitrile nanofiber membrane modified with chitosan molecules and immobilized with dye molecules. Reactive Orange 4, commercially known as Procion Orange MX2R, was found to be the best dye ligand for membrane chromatography. The dyed membrane was then employed to investigate the binding capacity of lysozyme under different operating parameters in batch mode. Furthermore, desorption of lysozyme using the P-Chitosan-Dye membrane was evaluated systematically. Both desorption rate and recovery percentage of lysozyme were fo...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Ng IS, Song CP, Ooi CW, Tey BT, Lee YH, Chang YK Tags: Int J Biol Macromol Source Type: research

Preparation, characterization and evaluation of cellulose nanocrystal/poly(lactic acid) in situ nanocomposite scaffolds for tissue engineering.
Abstract Cellulose nanocrystal (CNC)/poly(lactic acid) (PLA) in situ nanocomposite scaffolds were fabricated by in situ polymerization of lactic acid and CNC which was directly utilized as aqueous suspension, followed by a process of thermally induced phase separation. The CNC/PLA in situ nanocomposite porous scaffolds were characterized by mechanical test, protein adsorption, hemolysis test, in vitro degradation measurement, TEM, FTIR, SEM and WAXD. Compared to the PLA scaffold, the CNC/PLA in situ nanocomposite scaffolds showed a greatly increased compression modulus, an improved hemocompatibility and protein ad...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Luo W, Cheng L, Yuan C, Wu Z, Yuan G, Hou M, Chen JY, Luo C, Li W Tags: Int J Biol Macromol Source Type: research

Physicochemical characterization of Gracilaria chouae sulfated polysaccharides and their antioxidant potential.
Abstract A complete absence of the physicochemical characterization of Gracilaria chouae polysaccharides (GCP) and its corresponding bioactivities urged the need for this study. It was found that GCP is a heteropolysaccharide which exists in linear random coil conformation. It contained a sulfate content of 7.9% in addition to 52.63% total sugar content and 9.62% galacturonic acid. Galactose and 3,6-anhydrogalactose were found in a molar ratio of 1.0:0.6. Its setting and melting points were determined as 41.3 and 71.7 °C, respectively, which makes it a suitable candidate for industrial processing where furth...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Khan BM, Qiu HM, Wang XF, Liu ZY, Zhang JY, Guo YJ, Chen WZ, Liu Y, Cheong KL Tags: Int J Biol Macromol Source Type: research

A straightforward method for quantification of vinyl functionalized water soluble alginates via 13C NMR spectroscopy.
Abstract Alginates are fairly abundant in nature and possess many interesting properties, including their biocompatibility and ability to absorb large amounts of water. Hence, increasing interest in their derivatization has been observed and the determination of the number of newly introduced functionalities has become a key issue. For this purpose, literature generally reports on conventional 1H NMR spectra, typically recorded at elevated temperatures and/or after hydrolysis of the alginate to circumvent line broadening effects resulting from the high viscosity. The present work reports on the modification of alg...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Stubbe B, Graulus GJ, Reekmans G, Courtin T, Martins JC, Van Vlierberghe S, Dubruel P, Adriaensens P Tags: Int J Biol Macromol Source Type: research

Novel combinations of experimental and computational analysis tested on the binding of metalloprotoporphyrins to albumin.
Abstract The evidence that Human Serum Albumin (HSA) binds metal ions and organometallic compounds has generated interest in its physiological role as a metalloprotein and as a vehicle for synthetic biology applications (e.g., synthetic blood and solar energy conversion). HSA has been shown to bind metallo-porphyrins, however, the structural details of such interactions are available only for the HSA:heme complex. A typical challenge for studying the interaction of proteins with metalloporphyrins is the poor solubility of the ligands that affect the characterization the complexes. The manuscript shows that a combi...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Hu J, Soraiz EH, Johnson CN, Demeler B, Brancaleon L Tags: Int J Biol Macromol Source Type: research

Human papilloma virus DNA-biomarker analysis for cervical cancer: Signal enhancement by gold nanoparticle-coupled tetravalent streptavidin-biotin strategy.
Abstract Human papillomavirus (HPV) is a double-standard DNA virus, as well as the source of infection to the mucous membrane. It is a sexually transmitted disease that brings the changes in the cervix cells. Oncogenes, E6 and E7 play a pivotal role in the HPV infection. Identifying these genes to detect HPV strains, especially a prevalent HPV16 strain, will bring a great impact. Among different sensing strategies for pathogens, the dielectric electrochemical biosensor shows the potential due to its higher sensitivity. In this research, HPV16-E7 DNA sequence was detected on the carbodiimidazole-modified interdigit...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Lv Q, Wang Y, Su C, Lakshmipriya T, Gopinath SCB, Pandian K, Perumal V, Liu Y Tags: Int J Biol Macromol Source Type: research

Cellulose nanocrystals derived from Enteromorpha prolifera and their use in developing bionanocomposite films with water-soluble polysaccharides extracted from E. prolifera.
Abstract The biomass from Enteromorpha prolifera is used to exploit and transfer this common green bloom algae into new value-added products. In this work, cellulose nanocrystals (CNCs) were first prepared with the cellulose extracted from the biomass of E. prolifera. Initially, cellulose was treated by alkali and bleaching treatments. Subsequently, CNCs were isolated by acid hydrolysis with different concentrations. Structural changes and crystallinity were characterized by Fourier transform infrared spectroscopy (FTIR) spectroscopy, X-ray diffraction (XRD) analysis and t thermogravimetric analysis. The crystalli...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Kazharskay M, Ding Y, Arif M, Jiang F, Cong Y, Wang H, Zhao C, Liu X, Chi Z, Liu C Tags: Int J Biol Macromol Source Type: research

Redox-responsive blend hydrogel films based on carboxymethyl cellulose/chitosan microspheres as dual delivery carrier.
Abstract A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper. Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, Tetracycline Hydrochloride (TH) were used as model drugs. The prepared composite hydrogel films were characterized using FTIR, SEM, rheology measurement, mechanical and swelling tests. Our results showed that the well dispersed spherical CS microspheres endowed hydrogel film increased mechanic...
Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Wang F, Zhang Q, Li X, Huang K, Shao W, Yao D, Huang C Tags: Int J Biol Macromol Source Type: research

Investigating the effects of different natural molecules on the structure and oligomerization propensity of hen egg-white lysozyme.
Abstract The formation of amyloid aggregates is the hallmark of systemic and neurodegenerative diseases, also known as amyloidosis. Many proteins have been found to aggregate into amyloid-like fibrils and thus process is recognized as general tendency of polypeptides. Inhibition of protein aggregation and fibril formation is thus one of the important strategies in the prevention and treatment of such disease. There is a growing interest of identification of small molecules mainly natural compounds that can prevent or delay amyloid fibril formation. In this work, we report the effect of various compounds from diffe...
Source: International Journal of Biological Macromolecules - May 8, 2019 Category: Biochemistry Authors: Chaari A, Fahy C, Chevillot-Biraud A, Rholam M Tags: Int J Biol Macromol Source Type: research

Physical and functional characterization of succinoglycan exopolysaccharide produced by Rhizobium radiobacter CAS from curd sample.
Abstract Identification and rheological behaviour of succinoglycan exopolysaccharide (EPS) from Rhizobium radiobacter CAS isolated from curd was published in earlier reports. In current investigation physicochemical and functional properties of CAS EPS has been studied. SEC-MALLS revealed molecular weight (Mw), number molecular weight (Mn) and polydispersity index (PDI) of CAS EPS as 4.05 × 106 g·mol-1, 3.82 × 106 g·mol-1 and, 1.06 respectively. Thermogravimetric analysis showed the high thermal stability of CAS polymer where approximately 77% of CAS hydrocolloid maintain phys...
Source: International Journal of Biological Macromolecules - May 8, 2019 Category: Biochemistry Authors: Kavitake D, Delattre C, Devi PB, Pierre G, Michaud P, Shetty PH, Andhare P Tags: Int J Biol Macromol Source Type: research

Fabrication, characterization and drug loading efficiency of citric acid crosslinked NaCMC-HPMC hydrogel films for wound healing drug delivery applications.
In this study, hydrogel films were prepared from sodium carboxymethylcellulose (NaCMC) and hydroxypropylmethylcellulose (HPMC) using citric acid (CA) as crosslinker. The chemical crosslinking (ester bond) between NaCMC and HPMC was confirmed by FTIR analysis. It was found that swelling degree, crystallinity and water contact angle of hydrogel films based on NaCMC-HPMC (2 wt%) decrease with increase in CA from 5% to 20% (by weight). The increase in Tg and decrease in initial decomposition temperature were observed for increasing CA in NaCMC-HPMC films and were confirmed by DSC and TGA analysis, respectively. Tensile stren...
Source: International Journal of Biological Macromolecules - May 8, 2019 Category: Biochemistry Authors: Dharmalingam K, Anandalakshmi R Tags: Int J Biol Macromol Source Type: research

Corrigendum to "Sodium alginate/poly(vinyl alcohol)/nano ZnO composite nanofibers for antibacterial wound dressings" [Int. J. Biol. Macromol., 49 (2011) 247-254].
Corrigendum to "Sodium alginate/poly(vinyl alcohol)/nano ZnO composite nanofibers for antibacterial wound dressings" [Int. J. Biol. Macromol., 49 (2011) 247-254]. Int J Biol Macromol. 2019 May 07;: Authors: Shalumon KT, Anulekha KH, Nair SV, Nair SV, Chennazhi KP, Jayakumar R PMID: 31076181 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Shalumon KT, Anulekha KH, Nair SV, Nair SV, Chennazhi KP, Jayakumar R Tags: Int J Biol Macromol Source Type: research

Corrigendum to "Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels" [Int. J. Biol. Macromol., 67 (2014) 279-288].
Corrigendum to "Multifaceted chitin/poly(lactic-co-glycolic) acid composite nanogels" [Int. J. Biol. Macromol., 67 (2014) 279-288]. Int J Biol Macromol. 2019 May 07;: Authors: Rejinold NS, Biswas R, Chellan G, Jayakumar R PMID: 31076182 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Rejinold NS, Biswas R, Chellan G, Jayakumar R Tags: Int J Biol Macromol Source Type: research

Corrigendum to "Fabrication of chitosan/poly(caprolactone) nanofibrous scaffold for bone and skin tissue engineering" [Int. J. Biol. Macromol. 48 (2011) 571-576].
Corrigendum to "Fabrication of chitosan/poly(caprolactone) nanofibrous scaffold for bone and skin tissue engineering" [Int. J. Biol. Macromol. 48 (2011) 571-576]. Int J Biol Macromol. 2019 May 07;: Authors: Shalumon KT, Anulekha KH, Chennazhi KP, Tamura H, Nair SV, Jayakumar R PMID: 31076183 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Shalumon KT, Anulekha KH, Chennazhi KP, Tamura H, Nair SV, Jayakumar R Tags: Int J Biol Macromol Source Type: research

Corrigendum to "Biocompatible conducting chitosan/polypyrrole-alginate composite scaffold for bone tissue engineering" [Int. J. Biol. Macromol. 62 (2013) 465-471].
Corrigendum to "Biocompatible conducting chitosan/polypyrrole-alginate composite scaffold for bone tissue engineering" [Int. J. Biol. Macromol. 62 (2013) 465-471]. Int J Biol Macromol. 2019 May 07;: Authors: Sajesh KM, Jayakumar R, Nair SV, Chennazhi KP PMID: 31076184 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Sajesh KM, Jayakumar R, Nair SV, Chennazhi KP Tags: Int J Biol Macromol Source Type: research

Corrigendum to "Molecular insight into multiple RpoB clinical mutants of Mycobacterium tuberculosis: An attempt to probe structural variations in rifampicin binding site underlying drug resistance" [Int. J. Biol. Macromol. 120 (2018) 2200-2214].
Corrigendum to "Molecular insight into multiple RpoB clinical mutants of Mycobacterium tuberculosis: An attempt to probe structural variations in rifampicin binding site underlying drug resistance" [Int. J. Biol. Macromol. 120 (2018) 2200-2214]. Int J Biol Macromol. 2019 May 07;: Authors: Srivastava G, Tripathi S, Kumar A, Sharma A PMID: 31076185 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Srivastava G, Tripathi S, Kumar A, Sharma A Tags: Int J Biol Macromol Source Type: research