Improvement of Selenomonas ruminantium β-xylosidase thermal stability by replacing buried free cysteines via site directed mutagenesis.
Improvement of Selenomonas ruminantium β-xylosidase thermal stability by replacing buried free cysteines via site directed mutagenesis. Int J Biol Macromol. 2019 Jun 17;: Authors: Dehnavi E, Moeini S, Akbarzadeh A, Dabirmanesh B, Siadat SOR, Khajeh K Abstract β-xylosidase is an essential enzyme for breakdown of xylan to d-xylose. It has a significant potential application value for medicine, food, paper and pulp, and biofuel industries. Due to the negative consequences caused by buried free cysteine residues, mutational substitution of such residues is often accompanied by a notable increase...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Dehnavi E, Moeini S, Akbarzadeh A, Dabirmanesh B, Siadat SOR, Khajeh K Tags: Int J Biol Macromol Source Type: research

Functionalization of carbon nanotubes with chitosan based on MALI multicomponent reaction for Cu2+ removal.
Abstract In this work, we reported a novel "one-pot" strategy for preparation of chitosan-coated carbon nanotubes (CNTs) composites via a combination of Diels-Alder (DA) reaction and mercaptoacetic acid locking imine (MALI) reaction for the first time. To evaluate the adsorption characteristics, the as-prepared samples were applied to remove copper ions (Cu2+) from aqueous solution. The effects of contact time, solution pH, temperature and initial Cu2+ concentration on the adsorption of Cu2+ onto the as-prepared samples were investigated. The chitosan modified CNTs composites showed high affinity and fas...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Dou J, Gan D, Huang Q, Liu M, Chen J, Deng F, Zhu X, Wen Y, Zhang X, Wei Y Tags: Int J Biol Macromol Source Type: research

Partial characterization and antitumor activity of a polysaccharide isolated from watermelon rinds.
Abstract As a health-beneficial fruit, watermelon is widely consumed by people around the world. However, components responsible for the health benefits are not yet determined. As watermelon contains a large amount of polysaccharides, these carbohydrates might play an important role in the health benefits. In this work, polysaccharide from watermelon rinds (PWR) was extracted by papain digestion, purified and characterized by GC-MS, SEC/MALS/VD/DRI, FTIR and 1D and 2D NMR which revealed the glycosidic linkages, their locations in branches and backbone. The monosaccharide composition revealed that the extracted pol...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Dammak MI, Salem YB, Belaid A, Mansour HB, Hammami S, Le Cerf D, Majdoub H Tags: Int J Biol Macromol Source Type: research

Chicken egg yolk antibody (IgY) as diagnostics and therapeutics in parasitic infections - A review.
Abstract Early diagnosis and treatment of parasitic diseases are indispensable to combat parasites mediated morbidity and mortality in humans and animals. Mammalian sourced antibodies are being successfully used in immunotherapy and immunoassays. However, their increased conservation amongst mammals, involves them in unnecessary interaction and immune mediated pathologies, obstructing their applications in certain approaches in immunoassays. Further, the high production cost and difficulty to achieve high and stable antibody titer hampers their utility for therapeutic purposes. In recent years, chicken egg yolk im...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Diraviyam T, Ambi SV, Vieira-Pires RS, Xiaoying Z, Sekaran S, Krishnan U Tags: Int J Biol Macromol Source Type: research

Comprehensive analysis of lncRNA and mRNA expression profiles in macrophages activated by Actinidia eriantha polysaccharide.
This study further expanded current knowledge on the mechanisms of plant polysaccharides. PMID: 31220493 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Chen X, Du J, Zhu Y, Zhang C, Sun H Tags: Int J Biol Macromol Source Type: research

Efficient hydrolysis of wine and grape juice anthocyanins by Malbranchea pulchella β-glucosidase immobilized on MANAE-agarose and ConA-Sepharose supports.
Efficient hydrolysis of wine and grape juice anthocyanins by Malbranchea pulchella β-glucosidase immobilized on MANAE-agarose and ConA-Sepharose supports. Int J Biol Macromol. 2019 Jun 17;: Authors: Monteiro LMO, Pereira MG, Vici AC, Heinen PR, Buckeridge MS, de Lourdes Teixeira de Moraes Polizeli M Abstract β-glucosidases (BGLs) hydrolyze short-chain cellulooligosaccharides. Some BGLs can hydrolyze anthocyanins and be applied in the clarification process of food industries, especially grape juice and wine. Enzyme immobilization is a valuable tool to increase enzyme stabilization. In this wo...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Monteiro LMO, Pereira MG, Vici AC, Heinen PR, Buckeridge MS, de Lourdes Teixeira de Moraes Polizeli M Tags: Int J Biol Macromol Source Type: research

A porous collagen-carboxymethyl cellulose/hydroxyapatite composite for bone tissue engineering by bi-molecular template method.
Abstract Inspired by the mechanism of bone formation, a porous collagen-carboxymethyl cellulose/hydroxyapatite (Col-CMC/HA) composite was designed and fabricated using a biomimetic template of Col and CMC protein-polysaccharide bi-molecules. The morphology, composition and physical properties of Col-CMC/HA composites were characterized systematically. It was found that the nano-HA homogenously distributed on the surface of Col-CMC bi-templates while the composite presented 3D porous structure with pore size from 100 μm to 300 μm. The porosities of composites were located at the range of 71%-85%. Besides,...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: He X, Tang K, Li X, Wang F, Liu J, Zou F, Yang M, Li M Tags: Int J Biol Macromol Source Type: research

Innovative natural polymer metal nanocomposites and their antimicrobial activity.
Abstract Metal based nanocomposites are gaining popularity for the past few years due to their promising chemical and physical properties. These nanocomposites can be obtained by incorporation of metal nanoparticles with glass, ceramic and polymer. Metal polymer nanoparticles can be formed through direct reduction method, in situ methods like chemical reduction, photoreduction and thermal decomposition of metallic salt inside the polymer, ex-situ by direct insertion of metallic nanoparticles into the polymer, through vapor phase deposition techniques and ion implantation. Natural polymers such as cellulose, starch...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Zahran M, Marei AH Tags: Int J Biol Macromol Source Type: research

Improving physical properties and retrogradation of thermoplastic starch by incorporating agar.
, Móczó J Abstract To develop functional and sustainable packaging materials from starch and to enhance their properties, agar was added to thermoplastic corn starch (TPS) in a wide concentration range and the products were prepared either by casting or melt blending with a high glycerol content. The role of agar in the mechanical and barrier performance of films, as well as the compatibility of TPS and agar was systematically evaluated. In addition, the retrogradiation of starch in various blends after long storage periods was widely characterized. Results proved that the addition of agar to TPS res...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Fekete E, Bella É, Csiszár E, Móczó J Tags: Int J Biol Macromol Source Type: research

Structural characterization and anticoagulant activity of two polysaccharides from Patinopecten yessoensis viscera.
Abstract In the present study, two polysaccharides, SVP2-1 and SVP2-2, were isolated from Patinopecten yessoensis viscera and purified by using DEAE-52 cellulose and Sepharose CL-6B. Both SVP2-1 and SVP2-2 could extend activated partial thromboplastin time (APTT) and thrombin time (TT) and inhibit the transformation of fibrinogen into fibrin (FIB) concentration-dependently, indicating they inhibited clotting and thrombin through intrinsic and common pathways. Of note, SVP2-2 had stronger anticoagulant activity than SVP2-1, and its backbone was determined as →6)-α-Manp (1 → 2)-α-Galp(1 &...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Song S, Wang L, Wang L, Yu Q, Ai C, Fu Y, Yan C, Wen C, Zhu Z Tags: Int J Biol Macromol Source Type: research

Nonmulberry silk protein sericin blend hydrogels for skin tissue regeneration - in vitro and in vivo.
Abstract The damage to the skin is most prominent and evident as it is our first line of defense and unremittingly under attack by biological and environmental factors. The restoration of the skin is dependent on the extent of the injury. To explore the prospects of new biomimetic material, bi-layered skin construct is fabricated in vitro with nonmulberry silk protein sericin and chitosan hydrogels using human dermal fibroblasts and keratinocytes. The in vitro analysis of the hydrogels showed enhanced adhesion, proliferation, and migration of skin cells with coordinated interaction amongst themselves leading to th...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Sapru S, Das S, Mandal M, Ghosh AK, Kundu SC Tags: Int J Biol Macromol Source Type: research

Biologically active novel N, N, O donor tridentate water soluble hydrazide based O-carboxymethyl chitosan Schiff base Cu (II) metal complexes: Synthesis and characterisation.
Abstract Water soluble hydrazide based O-carboxymethyl chitosan Schiff base ligands have been prepared from chitosan and a mixture of benzoic acidhydrazide with β-diketone by carboxymethylation using mono chloroaceticacid (MCA) through in-situ reaction. The degree of deacetylation of chitosan was determined by elemental analysis and potentiometric titration. Schiff base ligands and the functional groups were characterised by FT-IR, 1H, and 13C NMR spectroscopic techniques. The crystallinity of ligands and complexes was evaluated by X-ray powder diffraction (XRD) studies. Thermal stability of all the synthesiz...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Manimohan M, Pugalmani S, Sithique MA Tags: Int J Biol Macromol Source Type: research

Binding and enzymatic properties of Ageritin, a fungal ribotoxin with novel zinc-dependent function.
This report highlights that Ageritin has the structure and function of an RNase but a Mg2+/Zn2+-dependent mechanism of action, a new finding for ribotoxins. As a zinc-dependent toxin, Ageritin can be classified among the arsenal of zinc-binding proteins involved in fungal virulence. PMID: 31220501 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Ruggiero A, García-Ortega L, Moreira M, Ragucci S, Landi N, Di Maro A, Berisio R Tags: Int J Biol Macromol Source Type: research

An alkali-extracted polysaccharide from Zizyphus jujuba cv. Muzao: Structural characterizations and antioxidant activities.
Abstract An acidic polysaccharide (SAZMP3) was isolated from jujube fruit residues by alkaline extraction and purified with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography. The structural characterizations of SAZMP3 were determined by high-performance gelpermeation chromatography, gas chromatography, Fourier transform infrared spectroscopy, methylation analysis, NMR spectroscopy, scanning electron microscopy, and atomic force microscopy. The results determined SAZMP3 was an acidic polysaccharide mainly contained rhamnose, galactose, and galacturonic acid with a molecular weight of 9.37 kDa and...
Source: International Journal of Biological Macromolecules - June 17, 2019 Category: Biochemistry Authors: Lin X, Ji X, Wang M, Yin S, Peng Q Tags: Int J Biol Macromol Source Type: research

Crystallographic structure and molecular dynamics simulations of the major endoglucanase from Xanthomonas campestris pv. campestris shed light on its oligosaccharide products release pattern.
pov I Abstract Cellulases are essential enzymatic components for the transformation of plant biomass into fuels, renewable materials and green chemicals. Here, we determined the crystal structure, pattern of hydrolysis products release, and conducted molecular dynamics simulations of the major endoglucanase from the Xanthomonas campestris pv. campestris (XccCel5A). XccCel5A has a TIM barrel fold with the catalytic site centrally placed in a binding groove surrounded by aromatic side chains. Molecular dynamics simulations show that productive position of the substrate is secured by a network of hydrogen bonds in th...
Source: International Journal of Biological Macromolecules - June 16, 2019 Category: Biochemistry Authors: Puhl AC, Prates ET, Rosseto FR, Manzine LR, Stankovic I, de Araújo SS, Alvarez TM, Squina FM, Skaf MS, Polikarpov I Tags: Int J Biol Macromol Source Type: research

Improved enzymatic hydrolysis of hardwood and cellulase stability by biomass kraft lignin-based polyoxyethylene ether.
Abstract Water-soluble kraft lignin-based polyoxyethylene ether (KL-PEG), synthesized from the black liquor of kraft pulping and PEG, was used to improve the enzymatic hydrolysis efficiency of dilute acid pretreated (DA-pretreated) Eucalyptus hardwood and cellulase stability. The physicochemical properties of KL-PEG polymer such as solubility, surface tension, charge and aggregation behavior in the solution were first studied. KL-PEG could enhance the enzymatic hydrolysis of Avicel and DA-pretreated Eucalyptus from 63.6% and 58.3% to 78.5% and 93.8%, respectively. The enzymatic activity of cellulase after the enzy...
Source: International Journal of Biological Macromolecules - June 16, 2019 Category: Biochemistry Authors: Lin X, Yang Y, Wu L, Wu L, Xu D, Qin Y Tags: Int J Biol Macromol Source Type: research

Adsorption and photocatalysis assisted optimization for drug removal by chitosan-glyoxal/Polyvinylpyrrolidone/MoS2 nanocomposites.
Abstract Chitosan-glyoxal/Polyvinylpyrrolidone/MoS2 (CSG/PVP/MoS2) nanocomposites were synthesized via hydrothermal-ultrasonic method. The CSG/PVP/MoS2 nano catalyst was characterized by XRD, UV-visible spectroscopy, FTIR, SEM and X-ray photoelectron spectroscopy, and diclofenac was used for the adsorption and photo-degradation test. SEM revealed the CSG/PVP/MoS2 nanocomposite has coarse and semi-flake structure and UV-vis absorbance spectra indicated its strong UV light response. Additionally, the CSG/PVP/MoS2 presents enhanced adsorption-photocatalytic activities in comparison to CSG/PVP, and the degradation was...
Source: International Journal of Biological Macromolecules - June 16, 2019 Category: Biochemistry Authors: Li X, Zhang Z, Fakhri A, Gupta VK, Agarwal S Tags: Int J Biol Macromol Source Type: research

Phenolic hydroxyl derived copper alginate microspheres as superior adsorbent for effective adsorption of tetracycline.
In this study, a novel phenolic hydroxyl (bayberry tannin) functionalized copper alginate microspheres adsorbent for the adsorption of tetracycline from contaminated water environment had been synthesized by a facile gelation and solidification process. The structure and chemical properties were characterized by the various analytical methods. The as-prepared adsorbent displayed the excellent adsorption performance with the maximum adsorption capacity of 153.89 mg·g-1, and the equilibrium data were well fitted the Langmuir isotherm model and pseudo-second-order kinetic model. In addition, the thermodynamic values ...
Source: International Journal of Biological Macromolecules - June 15, 2019 Category: Biochemistry Authors: Zhang X, Lin X, He Y, Luo X Tags: Int J Biol Macromol Source Type: research

Chitosan capped ZnO nanoparticles with cell specific apoptosis induction through P53 activation and G2/M arrest in breast cancer cells - In vitro approaches.
Abstract Now a days the well-organized strategy to induce apoptosis in cancer chemotherapy is to produce anti-cancer agent without any side effects is in needy. Hence the present investigation was aimed to explore the anticancer potentials of Amorphophallus paeoniifolius reduced zinc nanoparticles capped with chitosan against MCF 7 cell line (breast cancer cells)and studied for its optical and surface charge properties. The size, shape, dispersion and uniform distribution of biosynthesized zincoxide nanoparticle was examined using Field emission scanning electron microscope (FESEM) and Transmission electron micros...
Source: International Journal of Biological Macromolecules - June 15, 2019 Category: Biochemistry Authors: Anitha J, Selvakumar R, Murugan K Tags: Int J Biol Macromol Source Type: research

Improving stability of biosensor based on covalent immobilization of horseradish peroxidase by γ-aminobutyric acid and application in detection of H2O2.
Improving stability of biosensor based on covalent immobilization of horseradish peroxidase by γ-aminobutyric acid and application in detection of H2O2. Int J Biol Macromol. 2019 Jun 15;: Authors: Feizabadi M, Soleymanpour A, Faridnouri H, Ajloo D Abstract A novel electrochemical biosensor for the determination of hydrogen peroxide (H2O2) has been fabricated through covalently immobilized horseradish peroxidase (HRP) on modified multi walled carbon nanotube (MWCNT) by γ-aminobutyric acid (GABA) on glassy carbon electrode. Using the electrochemical techniques, the electrochemical properties...
Source: International Journal of Biological Macromolecules - June 15, 2019 Category: Biochemistry Authors: Feizabadi M, Soleymanpour A, Faridnouri H, Ajloo D Tags: Int J Biol Macromol Source Type: research

Effect of divalent cations on bovine serum albumin (BSA) and tannic acid interaction and its influence on turbidity and in vitro protein digestibility.
Abstract Tannins, proteins, and divalent cations interactions are important for many processes in the food industry and human and animal nutrition and health. The effect of magnesium, calcium, and manganese on the interaction, turbidity, and in vitro protein digestibility of bovine serum albumin and tannic acid complexes was studied. The divalent cations increase the affinity and influence the enthalpy and entropy changes of the protein and tannin binding. Magnesium maintained the nature of interactions, and calcium and manganese changed the binding mechanism. The factor that most influenced turbidity was the tann...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Kaspchak E, Goedert AC, Mafra LI, Mafra MR Tags: Int J Biol Macromol Source Type: research

Lactoferrin-phenothiazine dye interactions: Thermodynamic and kinetic approach.
In this study, the interactions between methylene blue (MB) or azure A (AZA) dyes and bovine lactoferrin (BLF) were investigated by surface plasmon resonance, fluorescence spectroscopy, and isothermal titration microcalorimetry. Despite the molecular similarities between the dyes, the BLF-AZA binding thermodynamic parameters (ΔGAZAo = -30.50 and ΔHAZAo = 10.8 (kJ·mol-1)) were higher in magnitude than those of the BLF-MB systems (ΔGMBo = -27.3 and ΔHMBo = 5.72 (kJ·mol-1)). To increase the systems' entropy (TΔSAZAo = 41.3 and TΔSMBo = 33.0 (kJ·mol-1)), the hydrophobic ...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Coelho YL, de Paula HMC, Agudelo AJP, Benhame AS, Hudson EA, Pires ACS, Silva LHM Tags: Int J Biol Macromol Source Type: research

Disassociation of β1-α1-β2 from the α2-α3 domain of prion protein (PrP) is a prerequisite for the conformational conversion of PrPC into PrPSc: Driven by the free energy landscape.
Disassociation of β1-α1-β2 from the α2-α3 domain of prion protein (PrP) is a prerequisite for the conformational conversion of PrPC into PrPSc: Driven by the free energy landscape. Int J Biol Macromol. 2019 Jun 14;: Authors: Chandrasekaran P, Santosh Kumar C, Rangachari K, Sekar K Abstract Misfolding of the cellular prion protein (PrPC) into β-sheet-rich scrapie form (PrPSc) is associated with transmissible spongiform encephalopathies. A point mutation F198S is responsible for the development of a rare inherited Gerstmann-Straussler-Scheinker disease caused by the aggr...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Chandrasekaran P, Santosh Kumar C, Rangachari K, Sekar K Tags: Int J Biol Macromol Source Type: research

Chemometrical-electrochemical investigation for comparing inhibitory effects of quercetin and its sulfonamide derivative on human carbonic anhydrase II: Theoretical and experimental evidence.
Abstract This paper reports results of a valuable study on investigation of inhibitory effects of the sulfonamide derivative of quercetin (QD) on human carbonic anhydrase II (CA-II) by electrochemical and chemometrical approaches. To achieve this goal, a glassy carbon electrode (GCE) was chosen as the sensing platform and different electrochemical techniques such as cyclic voltammetry (CV), differential pulse voltammetry (DPV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) were used to investigate and comparing inhibitory effects of quercetin (Q) and QD on CA-II. By the use of EQU...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Khodarahmi R, Khateri S, Adibi H, Nasirian V, Hedayati M, Faramarzi E, Soleimani S, Goicoechea HC, Jalalvand AR Tags: Int J Biol Macromol Source Type: research

Variants at position 603 of the CGTase from Bacillus circulans STB01 for reducing product inhibition.
Abstract Inhibition of cyclodextrin glycosyltransferases (CGTases) by their product cyclodextrins limits the efficiency of cyclodextrin production. In an effort to produce variants with good activity but reduced product inhibition, six mutants were constructed at position 603 of the CGTase from Bacillus circulans STB01, which exhibits mixed-type product inhibition. In a kinetic analysis, N603I showed reduced noncompetitive inhibition while N603K, N603H and N603R showed increased noncompetitive inhibition. Unexpectedly, N603E and N603D exhibited reductions in both competitive and noncompetitive product inhibition. ...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Chen S, Li Z, Gu Z, Hong Y, Cheng L, Li C Tags: Int J Biol Macromol Source Type: research

Pyrimidine biosynthesis in pathogens - Structures and analysis of dihydroorotases from Yersinia pestis and Vibrio cholerae.
Abstract The de novo pyrimidine biosynthesis pathway is essential for the proliferation of many pathogens. One of the pathway enzymes, dihydroorotase (DHO), catalyzes the reversible interconversion of N-carbamoyl-l-aspartate to 4,5-dihydroorotate. The substantial difference between bacterial and mammalian DHOs makes it a promising drug target for disrupting bacterial growth and thus an important candidate to evaluate as a response to antimicrobial resistance on a molecular level. Here, we present two novel three-dimensional structures of DHOs from Yersinia pestis (YpDHO), the plague-causing pathogen, and Vibrio ch...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Lipowska J, Miks CD, Kwon K, Shuvalova L, Zheng H, Lewiński K, Cooper DR, Shabalin IG, Minor W Tags: Int J Biol Macromol Source Type: research

Chondroitin sulfate immobilized PCL nanofibers enhance chondrogenic differentiation of mesenchymal stem cells.
Abstract Cold Atmospheric Plasma (CAP) is used as a promising method in surface modification for immobilization of chondroitin sulfate functional biomacromolecules on PCL nanofibrous substrates for cartilage tissue engineering. The GAG-grafted scaffolds are able to successfully support the attachment and proliferation of mesenchymal stem cells (MSCs). The seeded scaffolds show the chondro-differentiation of MSCs during a 21-days cell culture in a non-differential medium. Expression of SOX9, Collagen10 and Collagen2 proved the chondro-inductive effect of GAG-grafted scaffolds. Besides, no external chondro-genic dif...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Meghdadi M, Pezeshki-Modaress M, Irani S, Atyabi SM, Zandi M Tags: Int J Biol Macromol Source Type: research

The mitochondrial genome of the brown citrus aphid Aphis (Toxoptera) citricidus: Insights into the repeat regions in aphids and phylogenetic implications.
This study provides new insight into the evolution of aphid mt genomes, as well as useful information for resolving aphid phylogenetic questions. PMID: 31207332 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Wei DD, Lang N, Tao Y, He W, Tu YQ, Miao ZQ, Yang L, Wang JJ Tags: Int J Biol Macromol Source Type: research

Low-resolution SAXS and structural dynamics analysis on M. tuberculosis GmhB enzyme involved in GDP-heptose biosynthetic pathway.
Abstract The M. tuberculosis GmhB protein converts the d-glycero-α-d-manno-heptose 1,7-bisphosphate (GMB) intermediate into d-glycero-α-d-manno-heptose 1-phosphate by removing the phosphate group at the C-7 position. To understand the structure and substrate binding mechanism, the MtbGmhB was purified which elutes as monomer on gel filtration column. The small angle x-ray scattering analysis shows that MtbGmhB forms fully folded monomer with shape profile similar to its modeled structure. The circular dichroism analysis shows 38% α-helix, 15% β-sheets and 47% random coil structures in MtbGmh...
Source: International Journal of Biological Macromolecules - June 14, 2019 Category: Biochemistry Authors: Karan S, Pratap B, Ashish, Saxena AK Tags: Int J Biol Macromol Source Type: research

Physicochemical, thermal and mechanical characterization study of perimysial collagen of two bovine muscles.
In this study, chemical, thermal and mechanical characteristics were considered and quantified. However, the mechanical function and development of muscle in-vivo could be the main influence on the extracellular collagen characteristics as well as its interactions with non-collagen compounds. Its formation is essential for muscle function. PMID: 31202843 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Velázquez DE, Latorre ME Tags: Int J Biol Macromol Source Type: research

Structural characterization and immunoregulatory activity of two polysaccharides from the rhizomes of Atractylodes lancea (Thunb.) DC.
Abstract A neutral polysaccharide (ALP-1) and an acidic polysaccharide (ALP-3) were purified from Atractylodes lancea (Thunb.) DC. ALP-1 exhibited a linear backbone composed of (2 → 1)-linked β-d-fructofuranose. The backbone of ALP-3 was elucidated as →4)-GalAp-(1 → 3,4)-Rhap-(1→, and with branch chain substituted at O-3 position of →3,4)-Rhap-(1→. The branch chain consists of →3,5)-Araf-(1→, →5)-Araf-(1→, and Araf-(1→. Particularly, ALP-3 could significantly stimulate macrophage proliferation in a dose-dependent manner, and stimulate phagocytic, ...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Qin J, Wang HY, Zhuang D, Meng FC, Zhang X, Huang H, Lv GP Tags: Int J Biol Macromol Source Type: research

Partial characterization of the edible Spinacia oleracea polysaccharides: Cytoprotective and antioxidant potentials against Cd induced toxicity in HCT116 and HEK293 cells.
Abstract Spinacia is an interesting medicinal halophyte plant that is employed as a food and therapeutic agent in traditional medicine. In this work, water-soluble polysaccharides from Spinacia oleracea were extracted and preliminary characterization was established via FT-IR, UV-vis, 1H NMR and SEC/MALS DRI technics. The extracted polysaccharide, with an average molecular mass of 408 kDa, was composed of arabinose, galactose, mannose, glucose, rhamnose and xylose in the molar percentage of 49.3%, 28.1%, 4.9%, 7.8%, 8.2% and 1.7%, respectively. The polysaccharide showed significant antioxidant activity. Moreover...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Mzoughi Z, Souid G, Timoumi R, Le Cerf D, Majdoub H Tags: Int J Biol Macromol Source Type: research

Calcium and phosphorylation double-regulating caltractin initiating target protein XPC function.
Abstract Human centrin 2 (HsCen2) is a member of the EF-hand protein that plays a critical role in the centrosome duplication and separation during cell division. Reversible protein phosphorylation is an important regulatory mechanism for centrin. To obtain insight into the structural basis for the functional effects of phosphorylation, it was verified that the serine residue at position 170 of HsCen2 can be phosphorylated by protein kinase A (PKA) using 31P NMR spectroscopy. While the protein was phosphorylated, the extents of α-helix and the exposed hydrophobic surfaces on HsCen2 were decreased significant...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Zhao Y, Yang J, Chao J, Yang B Tags: Int J Biol Macromol Source Type: research

Impact of glazing based on chitosan-gelatin incorporated with Persian lime (Citrus latifolia) peel essential oil on quality of rainbow trout fillets stored at superchilled condition.
Abstract In this research, a new ice-glazing based on chitosan-gelatin (Ch-Gl) incorporated with Persian lime peel essential oil (LEO), and superchilled storage was established to assess the microbial, physicochemical, and sensory quality of rainbow trout fillets. The fillets were immersed in distilled water (control), Ch-Gl, Ch-Gl + 1% LEO, and Ch-Gl + 2% LEO to form glazing layer on the surface, then stored at -1.4 °C for 30 days. All treatments delayed the growth of total mesophilic bacteria, total psychrotrophic bacteria, Enterobacteriaceae, and lactic acid bacteria compared to control during t...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Sarmast E, Fallah AA, Dehkordi SH, Rafieian-Kopaei M Tags: Int J Biol Macromol Source Type: research

Thermostability improvement of the glucose oxidase from Aspergillus niger for efficient gluconic acid production via computational design.
In this study, we used in silico design to enhance the stability of glucose oxidase from Aspergillus niger. A combination of the best mutations increased the apparent melting temperature by 8.5 °C and significantly enhanced thermostability and thermoactivation. The variant also showed an increased optimal temperature without compromising the catalytic activity at lower temperatures. Moreover, the combined variant showed higher tolerance at pH 6.0 and 7.0, at which the wild-type enzyme rapidly deactivated. For GA production, an approximate 2-fold higher GA production yield was obtained, in which an almost complete c...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Mu Q, Cui Y, Tian Y, Hu M, Tao Y, Wu B Tags: Int J Biol Macromol Source Type: research

Structure characterization of a novel polysaccharide from Chinese wild fruits (Passiflora foetida) and its immune-enhancing activity.
Abstract A novel polysaccharide (PFP1) with an average molecular weight of 2.02 × 105 g/mol was isolated from Passiflora foetida fruits through hot water extraction, ethanol precipitation and column chromatography. The structure of PFP1 was determined by GPC-MALS-RI, IC, FT-IR, GC-MS and NMR. The structural analysis showed that PFP1 was a heteropolysaccharide and composed of mannose (48.83%), galactose (32.46%), glucose (6.21%), arabinose (5.88%), fructose (2.24%), galacturonic acid (2.20%), xylose (1.17%), fucose (0.17%), ribose (0.05%), and glucuronic acid (0.78%), with a backbone structure of →...
Source: International Journal of Biological Macromolecules - June 13, 2019 Category: Biochemistry Authors: Song Y, Zhu M, Hao H, Deng J, Li M, Sun Y, Yang R, Wang H, Huang R Tags: Int J Biol Macromol Source Type: research

Gamma radiation induced preparation of polyampholyte nanocomposite polymers for removal of Co(II).
Abstract A series of polyampholyte nanocomposite biopolymers, poly(N,N-diallyldimethylammonium chloride-co-acrylamide) grafted on carboxymethylcellulose/iron(III) oxide [P(DADMAC-AAm)CMC/Fe2O3] and poly(N,N-diallyldimethylammonium chloride-co‑sodium acrylate) grafted on carboxymethylcellulose/iron(III) oxide [P(DADMAC-SA)CMC/Fe2O3], was prepared with different molar ratios of anionic groups to cationic groups using gamma irradiation. The grafting properties and swelling behavior were investigated as a function of grafting conditions such as DADMAC, AAm, SA, and CMC concentrations and absorbed dose. Fourier trans...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Elsharma EM, Saleh AS, Abou-Elmagd WSI, Metwally E, Siyam T Tags: Int J Biol Macromol Source Type: research

Overcoming drug-resistance in lung cancer cells by paclitaxel loaded galactoxyloglucan nanoparticles.
This study involves the synthesis of PST-PTX nanoparticles using tamarind seed polysaccharide and Paclitaxel by epichlorohydrin crosslinking. The particles were further characterized by Dynamic Light Scattering (DLS), High-resolution transmission electron microscopy (HR-TEM) Fourier Transform Infrared Spectroscopy (FTIR) and UV-Visible spectroscopy. The cytotoxicity of PST-PTX nanoparticles in cancer cell lines and resistant cancer cell lines were determined by MTT assay. The quantitative analysis of cell death was determined by Annexin V dead cell assay, Caspase 3/7 assay and expression of pro-apoptotic protein Bax. The a...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Reshma PL, Unnikrishnan BS, Preethi GU, Syama HP, Archana MG, Remya K, Shiji R, Sreekutty J, Sreelekha TT Tags: Int J Biol Macromol Source Type: research

Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.
Abstract Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain. The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure. The NMR spectroscopy analysis revealed that EPS was a levan type EPS as a β-(2 → 6)-linked fructan. The FTIR analysis further confirmed the presence of the furanoid rings in the EPS structure. The levan S81 showed high level of thermal stability determined by DSC and TGA analysis. The lyophilised levan S81 showed a sheet-like...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Taylan O, Yilmaz MT, Dertli E Tags: Int J Biol Macromol Source Type: research

Purification, characterization and functional properties of exopolysaccharide from a novel halophilic Natronotalea sambharensis sp. nov.
Abstract An exopolysaccharide producing strain AK103T was isolated from Sambharlake, Rajasthan, India. Based on polyphasic taxonomy, the strain AK103T was found to be a novel species for which the name Natronotalea sambharensis sp. nov. is proposed. The strain was able to secrete (1.2 gL-1) EPS under optimum conditions. The FT-IR spectroscopy revealed that EPS had carboxyl, hydroxyl, carbonyl and sulfate ester groups. The high performance liquid chromatography (HPLC), gel permeation chromatography (GPC) and NMR revealed that the exopolysaccharide was composed of mannose, glucose and glucuronic acid with a molecula...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Singh S, Sran KS, Pinnaka AK, Choudhury AR Tags: Int J Biol Macromol Source Type: research

Adsorption of Cu(II) ions onto crosslinked Chitosan/Waste Active Sludge Char (WASC) beads: Kinetic, equilibrium, and thermodynamic study.
In this study, crosslinked chitosan/Waste Active Sludge Char (WASC) beads were developed as a novel composite adsorbent for the removal of Cu(II) ions from aqueous solution. The surface morphology and elemental composition of chitosan/WASC beads were characterized using Scanning Electron Microscopy (SEM). Adsorption isotherms of Cu(II) ions on adsorbents were correlated with the Langmuir and Freundlich models, and the data fitted better to the Freundlich isotherm model. The kinetic data were evaluated to calculate the theoretical metal uptake as a function of time. The highest correlation coefficient (R2) was calculated wi...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Dandil S, Sahbaz DA, Acikgoz C Tags: Int J Biol Macromol Source Type: research

Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification.
Abstract Enzymes can be very useful on exopolysaccharides (EPS) research, can be used at elucidation and also to modify the polysaccharides' structure in order to alter their physical properties. Thus, the reduction of the molecular mass could increase applications of these biopolymers. Therefore, the EPS production of different rhizobia and the action of xanthan lyase on its structures were evaluated. The strains produced significant amounts of EPS, and it was noticed that are heteropolysaccharides, composed galactose and glucose. Both EPS and xanthan were modified on β-glycosidic bonds, the mannose was remo...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: de Sousa BFS, Castellane TCL, Campanharo JC, de Macedo Lemos EG Tags: Int J Biol Macromol Source Type: research

New method for hydrogel synthesis from diphenylcarbazide chitosan for selective copper removal.
Abstract In this work, a simple method was developed for hydrogel preparation from 1,5-Diphenylcarbazide (DPC) and chitosan (CS) through Diazotization reaction of CS as a selective adsorbent (DPCCS) for Cu(II) ions. CS was treated with sodium nitrate and subsequent crosslinking reaction with DPC for the preparation of DPCCS. Different techniques were used for characterization of DPCCS. Various parameters such as, temperature, pH, and concentration of Cu(II) were used for adsorption studies. Kinetics of Cu(II) ion on DPCCS follows the Pseudo second order and equilibrium of adsorption occurs in short time. The equil...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Ahmad M, Zhang B, Wang J, Xu J, Manzoor K, Ahmad S, Ikram S Tags: Int J Biol Macromol Source Type: research

Modification of different molecular weights of chitosan by p-Coumaric acid: Preparation, characterization and effect of molecular weight on its water solubility and antioxidant property.
In this study, we modified three different molecular weights of chitosan by using p-Coumaric acid (p-CA) for enhancing their water solubility and antioxidant property. The chemical and physical properties of all native chitosan and its modified products were determined by Fourier transform spectroscopy (FTIR), ninhydrin assay, Folin-Ciocalteu reagent procedure, thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), high performance of liquid chromatography (HPLC), X-ray diffraction (XRD), water solubility and antioxidant property (both DPPH assay and reducing power assay). Results showed that the wate...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Panda PK, Yang JM, Chang YH, Su WW Tags: Int J Biol Macromol Source Type: research

A flaxseed heteropolysaccharide stimulates immune responses and inhibits hepatitis B virus.
Abstract A novel heteropolysaccharide defined as FP-1 was enriched from flaxseed hull through hot water extraction (boiling water, 2 h, twice) followed by ion-exchange and size exclusion chromatography. FP-1 is a highly purified heteropolysaccharide with an average molecular weight of 2626 kDa. Its monosaccharide composition includes xylose (35.2%), rhamnose (23.8%), galactose (23.4%), arabinose (10.6%), fucose (4.0%), glucose (2.7%) and mannose (0.3%). Main linkage type of FP-1 backbone includes →2)-α-L-Rhap-(1→, →4)-α-D-GalpA-(1→, →4)-β-D-Xylp-(1→, →3,5)-&a...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Liang S, Li X, Ma X, Li A, Wang Y, Reaney MJT, Shim YY Tags: Int J Biol Macromol Source Type: research

Application of cress seed musilage magnetic nanocomposites for removal of methylene blue dye from water.
Abstract A magnetic nanocomposite adsorbent based on cress seed mucilage (CSM) was synthesized for removing methylene blue (MB) cationic dye from aqueous solutions. These adsorbent nanoparticles were prepared by in situ formation of magnetic iron oxide nanoparticles (MIONs) coupled with CSM mucilage and benefited from the advantages of both CSM and MIONs. The CSM-MIONs adsorbent exhibited a great dye adsorption capacity along with a strong magnetic character. The effect of various experimental parameters were studied on the adsorption performance of CSM-MIONs including pH, ionic strength, initial dye concentration...
Source: International Journal of Biological Macromolecules - June 12, 2019 Category: Biochemistry Authors: Allafchian A, Mousavi ZS, Hosseini SS Tags: Int J Biol Macromol Source Type: research

Improving the cellobiose-hydrolysis activity and glucose-tolerance of a thermostable β-glucosidase through rational design.
This study provides insights into enzymatic properties of Bgl1317 for engineering it into a powerful catalyst and β-glucosidases in general. PMID: 31199970 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - June 11, 2019 Category: Biochemistry Authors: Liu X, Cao L, Zeng J, Liu Y, Xie W Tags: Int J Biol Macromol Source Type: research

Naja atra cardiotoxins enhance the protease activity of chymotrypsin.
Abstract Snake venom cardiotoxins (CTXs) present diverse pharmacological functions. Previous studies have reported that CTXs affect the activity of some serine proteases, namely, chymotrypsin, subtilisin, trypsin, and acetylcholinesterase. To elucidate the mode of action of CTXs, the interaction of CTXs with chymotrypsin was thus investigated. It was found that Naja atra CTX isotoxins concentration-dependently enhanced chymotrypsin activity. The capability of CTX1 and CTX5 in increasing chymotrypsin activity was higher than that of CTX2, CTX3, and CTX4. Removal of the molecular beacon-bound CTXs by chymotrypsin, c...
Source: International Journal of Biological Macromolecules - June 11, 2019 Category: Biochemistry Authors: Shi YJ, Huang CH, Lee YC, Wang LJ, Chiou JT, Chang LS Tags: Int J Biol Macromol Source Type: research

Disruption of R867 and Y613 interaction plays key roles in JAK2 R867Q mutation caused acute leukemia.
In this study, homology modeling analysis showed that loss of interaction between R867 and Y613 disrupted the JAK2 JH1/JH2 domain's interactions was responsible for its activation. JAK2 R867Q and mutations (R867A and R867G) abolished this interaction caused JAK2 constitutive activation. While, mutations (R867K, Y613E, R867K/Y613E) repairing this interaction reduced JAK2 R867Q mutation's activity. Furthermore, our studies showed that abolished R867 and Y613 interaction disrupted JH1/JH2 domains' interactions and led to JAK2 constitutive activation. More importantly, mutations (R867Q, R867A and R867G) disrupted this interact...
Source: International Journal of Biological Macromolecules - June 11, 2019 Category: Biochemistry Authors: Wu QY, Ma MM, Zhang S, Cao J, Yan ZL, Chen C, Li ZY, Zeng LY, Wang XY, Li F, Xu KL Tags: Int J Biol Macromol Source Type: research

Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity.
Abstract Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant. Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.28% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50. The extraction yields of the poly...
Source: International Journal of Biological Macromolecules - June 11, 2019 Category: Biochemistry Authors: Lin Y, Zeng H, Wang K, Lin H, Li P, Huang Y, Zhou S, Zhang W, Chen C, Fan H Tags: Int J Biol Macromol Source Type: research