Expression of a recombinant Lentinula edodes cellobiohydrolase by Pichia pastoris and its effects on in vitro ruminal fermentation of agricultural straws.
This study demonstrates that LeCel7A could enhance the hydrolysis and in vitro ruminal fermentation of agricultural straws, showing the potential of LeCel7A for improving the utilization of agricultural straws in ruminants. PMID: 31077694 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Li L, Qu M, Liu C, Pan K, Xu L, OuYang K, Song X, Li Y, Zhao X Tags: Int J Biol Macromol Source Type: research

Effects of macromolecular humic/fulvic acid on Cd(II) adsorption onto reed-derived biochar as compared with tannic acid.
Abstract In this work, the adsorption of Cd(II) on reed-derived biochar (BC) as affected by dissolved organic matter (DOM), i.e., tannic acid (TA), macromolecular fulvic acid (FA) and humic acid (HA), was investigated through batch experiments. Results showed that a promotive effect of DOM on Cd(II) removal was demonstrated at pH  6. The maximum Cd(II) adsorption capacities were found to be 206.18 mg/g based on the Sips model. The order of addition sequences was found to exert a significant influence on Cd(II) adsorption. Addition of DOM (100 mg/L) to a Cd(II)-BC pre-equilibrated system led to 1.3%-7.4% en...
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Wang Y, Li Y, Zhang Y, Wei W Tags: Int J Biol Macromol Source Type: research

Involvement of a TNF homologue in balancing the host immune system of Macrobrachium nipponense.
Abstract In vertebrates, tumor necrosis factors (TNFs) are well-known cytokines involved in a diversity of physiological and pathological events. In the present work we identified a novel TNF-like gene (MnTNF) from Macrobrachium nipponense, which codes for a protein sharing detectable sequence identify (26-27%) to the mammalian TNFSF members, TWEAK and EDA. Tissue distribution analysis indicated that MnTNF was predominantly expressed in nervous tissue, and at relatively high level in haemocytes, gill, intestine and muscle, whereas was almost undetectable in hepatopancreas. In gill, MnTNF was significantly up-regul...
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Qin N, Tang T, Liu X, Xie S, Liu F Tags: Int J Biol Macromol Source Type: research

Preparation and characterization of active and intelligent packaging films based on cassava starch and anthocyanins from Lycium ruthenicum Murr.
Abstract Lycium ruthenicum Murr. is a functional food with abundant anthocyanins. Since anthocyanins can change colors under different pH conditions, pH-sensitive packaging films were developed based on cassava starch and L. ruthenicum anthocyanins (LRA). Effect of LRA content on the physical, structural, antioxidant and pH-sensitive properties of starch-LRA films were evaluated. In addition, starch-LRA films were applied to monitor the freshness of pork. Spectroscopic analysis showed LRA contained six kinds of anthocyanins. The incorporation of LRA significantly enhanced the water vapor and ultraviolet-visible li...
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Qin Y, Liu Y, Yong H, Liu J, Zhang X, Liu J Tags: Int J Biol Macromol Source Type: research

Physicochemical and in vitro digestion of millet starch: Effect of moisture content in microwave.
In conclusion, high moisture content can enhance the effect of the microwave to millet starch. PMID: 31075330 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Li Y, Hu A, Wang X, Zheng J Tags: Int J Biol Macromol Source Type: research

Eco-compatible cassava starch films for fertilizer controlled-release.
;a MA Abstract As an attempt to tackle the environmental issues derived from the use of non-degradable plastic and fertilizers overuse, such as irreversible soil pollution, this work focuses on the development of a fertilizer controlled-release, bio-based and biodegradable composite films for agricultural applications. Cassava starch and bagasse composite materials containing urea as active compound and plasticizer were prepared to achieve this purpose, analyzing their properties and stability during storage. Moreover, their biodegradation rate and urea release in soil were also assessed. Films were based on cassa...
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Versino F, Urriza M, García MA Tags: Int J Biol Macromol Source Type: research

Enhanced catalytic activity of nanosilver with lignin/polyacrylamide hydrogel for reducing p-nitrophenol.
Abstract Lignin, as the second largest natural polymer in nature, has great practical application value. Three-dimensional silver/lignin/PAM hydrogels have been successfully prepared via a rapid and convenient assembly process, showing good catalytic hydrogenation ability and stability in batch and dynamic catalytic processes of p-nitrophenol. It can be seen from the characterization results that abundant amino groups in the catalyst carrier can disperse silver ions homogeneously and limit the growth of silver nanoparticles in the reduction process with sodium borohydride. At room temperature, the catalytic proces...
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Gao C, Wang X, Zhai S, An Q Tags: Int J Biol Macromol Source Type: research

The protective effects of a novel polysaccharide from Lentinus edodes mycelia on islet β (INS-1) cells damaged by glucose and its transportation mechanism with human serum albumin.
The protective effects of a novel polysaccharide from Lentinus edodes mycelia on islet β (INS-1) cells damaged by glucose and its transportation mechanism with human serum albumin. Int J Biol Macromol. 2019 May 07;: Authors: Cao XY, Liu D, Bi RC, He YL, He Y, Liu JL Abstract High glucose can lead to toxicity on islet β cells. The protective effects of a novel Lentinus edodes mycelia polysaccharide (LMP) on INS-1 cells damaged by glucose were investigated. Cell viability, lactate dehydrogenase (LDH) release, cell apoptosis, intracellular reactive oxygen species (ROS), superoxide dismutase (SO...
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Cao XY, Liu D, Bi RC, He YL, He Y, Liu JL Tags: Int J Biol Macromol Source Type: research

Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.
Abstract Hydroxyapatite was successfully synthesized using eggshell waste as a raw material. Eggshell waste and orthophosphoric acid were co-precipitated for 2 h at an ambient temperature. The pH of the solution was adjusted to 10 using ammonium hydroxide. Then, 10-30 wt% of hydroxyapatite was loaded into the chitosan film. Scanning electron microscopy and energy dispersive analyses confirmed the morphological properties and dispersion. A thermogravimetric analysis showed a significant enhancement in the thermal stability of the composite. The existence of hydroxyapatite resulted in a higher thermal stability....
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Trakoolwannachai V, Kheolamai P, Ummartyotin S Tags: Int J Biol Macromol Source Type: research

Fabrication of MnFe2O4 nanoparticles embedded chitosan-diphenylureaformaldehyde resin for the removal of tetracycline from aqueous solution.
In this study, a magnetic composite was synthesized using chitosan, diphenylurea and formaldehyde with magnetic nanoparticles, (MnFe2O4) via condensation polymerization. The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc. To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several fact...
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Ahamad T, Ruksana, Chaudhary AA, Naushad M, Alshehri SM Tags: Int J Biol Macromol Source Type: research

Cloning, purification, structural, and functional characterization of methicillin-resistant Staphylococcus aureus (MRSA252) RsbV protein.
Abstract The MRSA252 (Methicillin-resistant Staphylococcus aureus) σB factor dependent transcription is carried out in the presence of activated anti-σB factor antagonist (RsbV) with anti-σB factor (RsbW). The RsbV protein is expressed under stress to help bacterial cell survival. Since in-silico structure-based drug design and computational studies limit the search of inhibitors as protein flexibility, molecular conformation and promiscuity are bottle neck for prediction tools. Therefore, there was a need to clone, and purify RsbV protein to carry out in-vitro experiments. The recombinant RsbV p...
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Durr-E-Shahwar S, Atia-Tul-Wahab, Choudhary MI, Jabeen A Tags: Int J Biol Macromol Source Type: research

Antioxidant activities of Sagittaria sagittifolia L. polysaccharides with subcritical water extraction.
Abstract In the present study, orthogonal experiment (L9 (3)4) was used to optimize the extraction process of Sagittaria sagittifolia L. polysaccharides (SSP) with subcritical water, and the antioxidant activities of polysaccharides were also investigated. The results showed that the optimum extraction conditions were obtained when pH, extraction temperature, extraction time, and liquid to solid ratio were 7, 170 °C, 16 min and 30:1 (mL/g), respectively. In addition, DPPH/ABTS radical scavenging rate and Fe3+ reducing power of polysaccharides exhibited a certain dose-effect relationship. Furthermore, both ...
Source: International Journal of Biological Macromolecules - May 7, 2019 Category: Biochemistry Authors: Zhang J, Wen C, Chen M, Gu J, Zhou J, Duan Y, Zhang H, Ma H Tags: Int J Biol Macromol Source Type: research

Preparation of nano-alkalinecellulose carboxylates (NACCs) as the methylene blue sorbent and as the catalyst for the large-scale nifedipine synthesis.
Abstract In this work, various new nano-alkalinecellulose carboxylates (NACCs) were prepared by oxidative hydrolysis of microalkalinecellulose (MACp) given from prewashed cotton. After the optimization of the reaction time, temperature, oxidant system, and oxidant loading, the new flake-shape anionic NACC24 with base capacity 10.8 mmol HO-/g was obtained from MACp at 50 °C. The characterized NACC24 and MACp were comparatively used as bio-sorbent for removal of methylene blue (MB) from wastewater and as the catalyst in the large-scale synthesis of nifedipine. With the Langmuir model and monolayer pseudo-sec...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Tamaddon F, Arab D Tags: Int J Biol Macromol Source Type: research

Preparation and characterization of antioxidant and antimicrobial packaging films based on chitosan and proanthocyanidins.
Abstract The antioxidant and antimicrobial food packaging films were prepared by using chitosan (CS) and proanthocyanidins (PA). Effect of PA content (0, 5, 10, 15 and 20 wt% of PA on CS basis) on the physical, antioxidant and antimicrobial properties of CS-based films were determined. The prepared CS-PA films showed a brown color. PA incorporation remarkably increased the thickness, water solubility, water vapor permeability, opacity, tensile strength and thermal stability of CS film; whereas significantly reduced the moisture content, oxygen permeability, UV-vis light transmittance, elongation at break and cry...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Bi F, Zhang X, Bai R, Liu Y, Liu J, Liu J Tags: Int J Biol Macromol Source Type: research

Mitochondrial genomes of three kissing bugs (Reduviidae: Triatominae) and their phylogenetic implications.
Abstract The reduviid subfamily Triatominae, also called kissing bugs, are vectors of Chagas disease, which is one of the most seriously neglected tropical parasitic diseases. Only three complete mitochondrial genomes of kissing bugs from the genus Triatoma have been sequenced to date. To better understand the diversity of mitochondrial genomes and the evolution of kissing bugs, mitochondrial genomes of three kissing bugs, Triatoma migrans, Panstrongylus rufotuberculatus, and Rhodnius pictipes, were sequenced using next-generation sequencing and a comparative mitochondrial genomic analysis of three genera and two ...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Zhao Y, Jiang M, Wu Y, Song F, Cai W, Li H Tags: Int J Biol Macromol Source Type: research

The utilization of low molecular weight heparin-poloxamer associated Laponite nanoplatform for safe and efficient tumor therapy.
Abstract In the present investigation, we have synthesized the dalteparin which is a kind of low molecular weight heparin (LMWH) and possess the antitumor and antiangiogenic efficacy with carboxylates poloxamer 407 to form a heparin-poloxamer nanogel (HP copolymer). The complex HP is capable of enhancing the efficacies, minimizing the side effects of dalteparin and exhibiting a good thermosensitivity. Therewith, the synthetic Laponite RDS (LR) nanosilicate embarked with doxorubicin (DOX) at a satisfactory high drug entrapment efficiency was integrated with the complex HP and thus constituted a newfangled biocompat...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Li J, Pan H, Qiao S, Li Y, Wang J, Liu W, Pan W Tags: Int J Biol Macromol Source Type: research

Manufacturing and physical characterization of absorbable oxidized regenerated cellulose braided surgical sutures.
The objective of this article was to prepare novel braided sutures by TEMPO-mediated oxidation regenerated cellulose (TORC) to achieve a suturable material with biodegradability and ideal mechanical properties. Regenerated cellulose (RC) strands were made into sutures on a circular braiding machine, and TEMPO-mediated oxidation treatment was introduced alternatively after braiding. The RC sutures under different oxidation time were characterized by ATR-FTIR, electrical conductivity, XRD analysis, physical properties and in vitro degradation property. We further demonstrate that the RC sutures were oxidized and formed the c...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Li H, Cheng F, Chávez-Madero C, Choi J, Wei X, Yi X, Zheng T, He J Tags: Int J Biol Macromol Source Type: research

The role of TNF- α and TLR4 polymorphisms in the placenta of pregnant women complicated by preeclampsia and in silico analysis.
CONCLUSION: Our study for the first time presented evidence on the association of the placental TNF-α -308GA genotype and TNF-α -238A allele with decreased risk of PE. PMID: 31071395 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Mohammadpour-Gharehbagh A, Jahantigh D, Eskandari M, Eskandari F, Rezaei M, Moghaddam SZ, Teimoori B, Salimi S Tags: Int J Biol Macromol Source Type: research

Macrophage immunomodulatory activity of the cationic polymer modified PLGA nanoparticles encapsulating Alhagi honey polysaccharide.
Abstract In previous researches, the results showed that Alhagi honey polysaccharide-loaded poly(D,L-lactic-co-glycolic) acid nanoparticles (AHPP) as immune adjuvant enhanced Th1 immune responses. In order to further enhance the immune adjuvant activity and phagocytosis of the nanoparticles, three kinds of Alhagi honey polysaccharide-loaded cationic polymer modified PLGA nanoparticles were prepared to investigate the effects on macrophages in vitro. After treatment with the nanoparticles, the effects of phagocytosis, co-stimulatory molecules expression, nitric oxide (NO), inducible nitric oxide synthase (iNOS), an...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Wusiman A, He J, Zhu T, Liu Z, Gu P, Hu Y, Liu J, Wang D Tags: Int J Biol Macromol Source Type: research

An assessment of antibacterial mode of action of chitosan on Listeria innocua cells using real-time HATR-FTIR spectroscopy.
Abstract The antibacterial mode of action of chitosan using real-time, horizontal attenuated total reflectance, Fourier-transform infrared (HATR-FTIR) spectroscopy and transmission electron microscopy (TEM) was investigated. Listeria innocua was treated with chitosan solution. HATR-FTIR revealed an increased lethality and substantially metabolomics response on cell components. The main changes in FTIR and their 2nd derivative spectra were at 1045 cm-1 (carbohydrates in cell wall). Principal component analysis clearly segregated untreated and treated cells. Loadings plot revealed the functional groups in cell wal...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Tantala J, Thumanu K, Rachtanapun C Tags: Int J Biol Macromol Source Type: research

Antioxidation, hepatic- and renal-protection of water-extractable polysaccharides by Dictyophora indusiata on obese mice.
Abstract The present work aimed to investigate the antioxidant, hepatic and renal protective effects of water-extractable polysaccharides (WPS) by Dictyophora indusiata fruiting body on high-fat emulsion-induced obese mice. The structural analysis indicated that WPS was the α-configurational heteropolysaccharide with the major monosaccharides of mannose and glucose, and the polydispersity of 1.77. The in vivo results showed that WPS administration could improve obesity-associated hepatic and renal metabolic impairment, reduce body weight and ameliorate oxidative stress of liver and kidney by down-regulating ...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Wang W, Song X, Zhang J, Li H, Liu M, Gao Z, Wang X, Jia L Tags: Int J Biol Macromol Source Type: research

Low cost hydrogels based on gum Tragacanth and TiO2 nanoparticles: production and RBFNN modelling of methylene blue dye removal.
In this study, low-cost and high adsorption capacity hydrogels based on gum tragacanth biopolymer (GT) and TiO2 nanoparticles were produced by using glutaraldehyde as the crosslinking agent. These hydrogels were applied in photocatalytic process to remove methylene blue from simulated colored solution. TiO2- Gum Tragacanth hydrogels (TGTH) were characterized by FESEM and FTIR to investigate the surface morphology and functional group of the synthesized hydrogel. Contact angle measurements showed that, the hydrophilicity nature of crosslinked TGTH decreased compared to GT films. The effect of particle size, initial dye conc...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Ranjbar-Mohammadi M, Rahimdokht M, Pajootan E Tags: Int J Biol Macromol Source Type: research

Inhibiting c-Jun N-terminal kinase (JNK)-mediated apoptotic signaling pathway in PC12 cells by a polysaccharide (CCP) from Coptis chinensis against Amyloid- β (Aβ)-induced neurotoxicity.
In this study, we investigated the protective effect and possible mechanism of a polysaccharide (CCP) from Coptis chinensis against Amyloid-β protein (Aβ)-induced toxicity in PC12 cells. The results showed pretreatment with CCP significantly protected PC12 cells from Aβ25-35 induced cell death, lactate dehydrogenase (LDH) release, nuclear fragmentation, mitochondrial dysfunction and cytochrome c release from mitochondria. Furthermore, CCP (100 μg/ml) significantly inhibited Aβ25-35 induced c-Jun N-terminal kinase (JNK) phosphorylation, but not influence signal-regulated kinase (ERK) and P38 mitogen...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Li Y, Liu C, Zhu X, Zhang P, Yu H, Li Y, Li Z, Li M Tags: Int J Biol Macromol Source Type: research

Effects of crotoxin, a neurotoxin from Crotalus durissus terrificus snake venom, on human endothelial cells.
Abstract Vascular endothelium plays an important modulatory role due to the production of molecules that mediate vasomotricity, inflammation, and leukocyte adhesion and rolling. Here we addressed whether crotoxin (25-200 μg/mL) - the main component of Crotalus durissus terrificus snake venom - interferes with cell viability, apotosis/necrosis, and cell response to oxidative stress in human umbilical vein endothelial cells (HUVEC) in vitro. We also examined whether crotoxin alters the levels of interleukins, adhesion molecules, and endothelial vasoactive factors in HUVEC cells treated or not with lipopolysacch...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: de Andrade CM, Rey FM, Cintra ACO, Sampaio SV, Torqueti MR Tags: Int J Biol Macromol Source Type: research

Encapsulation and release behavior of curcumin based on nanoemulsions-filled alginate hydrogel beads.
Abstract To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation. The encapsulation and release behavior of curcumin (Cur) were further investigated. The results indicated that Cur packaged nanoemulsions were with the size of 24.26 ± 0.22 nm. The nanoemulsions filled SA hydrogel beads were spherical shell with the diameter of 0.46 ± 0.02 mm. For Cur, the EE and LC of emulsion filled SA hydrogel beads were 99.15 &p...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Xu W, Huang L, Jin W, Ge P, Shah BR, Zhu D, Jing J Tags: Int J Biol Macromol Source Type: research

Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra).
Abstract Chondroitin sulfate/dermatan sulfate (CS/DS) were isolated and purified for the first time from the bone of corb (Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed. Infrared spectrum and agarose-gel electrophoresis for extracted CS/DS were also investigated. The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa. Disaccharide analysis indicated that CBG was composed of monosulfated disaccharides in positions 6 and 4 of the N-acetylgalactosamine (8.6% and 40.0%, respectively)...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Bougatef H, Krichen F, Capitani F, Amor IB, Gargouri J, Maccari F, Mantovani V, Galeotti F, Volpi N, Bougatef A, Sila A Tags: Int J Biol Macromol Source Type: research

Physicochemical, thermal and rheological properties of synthesized carboxymethyl tara gum (Caesalpinia spinosa).
Abstract Carboxymethyl tara gum (CMTG) was synthesized from the reaction between tara gum (TG) and monochloroacetic acid (MCA) in the presence of sodium hydroxide. The modification reaction was optimized in terms of the MCA/NaOH ratio, reaction time and temperature evaluated for degree of substitution (DS). The etherification was confirmed by FTIR and 13C NMR spectroscopy, and it was characterized by different analyses. After carboxymethylation, CMTG showed new bonds at 1592, 1413 and 1320 cm-1 by FTIR and a new peak at δ = 178 ppm by 13C NMR in response to the insertion of the carbonyl group. The mi...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Santos MB, Dos Santos CHC, de Carvalho MG, de Carvalho CWP, Garcia-Rojas EE Tags: Int J Biol Macromol Source Type: research

Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing.
Abstract To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique. The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG). FTIR and H-NMR studies indicated ester reactions between carboxyl...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Azarniya A, Tamjid E, Eslahi N, Simchi A Tags: Int J Biol Macromol Source Type: research

Efficient removal of lead and copper ions from water by enhanced strength-toughness alginate composite fibers.
In this study, we designed and synthesized an enhanced strength-toughness alginate composite fiber by using graphene oxide as reinforcing filler for removing heavy metal ions from water media. The as-prepared alginate composite fiber exhibits high affinity to Pb2+ ion, and the maximum adsorption capacity for Pb2+ reached 386.2 mg/g, which is higher than the reported Pb2+-sorbent. The prepared round-shaped nanofibers have relatively uniform distribution with a diameter of 400 nm, and the interlaced fibers form porous structure that conducive to the rapid transport of heavy metal ions. Adsorption mechanism analysis shows...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Pan L, Wang Z, Zhao X, He H Tags: Int J Biol Macromol Source Type: research

Crotoxin, a neurotoxin from Crotalus durissus terrificus snake venom, as a potential tool against thrombosis development.
Abstract Endothelium plays an important modulatory role due to the synthesis and secretion of molecules that act on hemostasis and fibrinolysis. As there are no literature data about the effect of crotoxin (CTX) - the main component of Crotalus durissus terrificus snake venom - on human endothelial cells, the present study examined how CTX (25-200 μg/mL) affects the endothelial production of the hemostatic factors antithrombin III, protein C, protein S, plasminogen activator inhibitor-1 (PAI-1), tissue plasminogen activator (t-PA), and vWF in human umbilical vein endothelial cells (HUVEC) in vitro. Production...
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: De Andrade CM, Rey FM, Bianchini FJ, Sampaio SV, Torqueti MR Tags: Int J Biol Macromol Source Type: research

Fabrication of antimicrobial composite films based on xylan from pulping process for food packaging.
In conclusion, the XL/HEC antimicrobial film has great potential in the field of sustainable food packing materials. PMID: 31071408 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 6, 2019 Category: Biochemistry Authors: Yang YC, Mei XW, Hu YJ, Su LY, Bian J, Li MF, Peng F, Sun RC Tags: Int J Biol Macromol Source Type: research

A natural cordycepin/chitosan complex hydrogel with outstanding self-healable and wound healing properties.
Abstract The chitosan-based hydrogel dressings have been intensively engaged in wound healing. In respect to the relatively unsatisfied anti-bacterial performance of chitosan, we developed a self-healable cordycepin (abbreviated to CY)/chitosan (abbreviated to CS) hydrogel dressing cross linking by non-covalent bonds (hydrogen bond and electrostatic interaction) for wound healing through one-step 'freeze-thaw' method without adding any cross-linking agent. The as-prepared hybrid hydrogel exhibited excellent biocompatibility, suitable swelling ratio and desired mechanical strength, providing remarkable antimicrobia...
Source: International Journal of Biological Macromolecules - May 5, 2019 Category: Biochemistry Authors: Song R, Zheng J, Liu Y, Tan Y, Yang Z, Song X, Yang S, Fan R, Zhang Y, Wang Y Tags: Int J Biol Macromol Source Type: research

Statistical optimization, physio-chemical and bio-functional attributes of a novel exopolysaccharide from probiotic Streptomyces griseorubens GD5.
In this study, we assessed the bio-functional attributes of an exopolysaccharide (EPS) produced by the actinobacterial probiont Streptomyces griseorubens GD5. The EPS production was doubled to 9.50 g/L by varying the concentration of media components using Response surface methodology. The physio-chemical properties of GD5EPS were examined by various analytical techniques. Several functional groups were detected in FT-IR spectra, including β-glycosidic linkages, hydroxyl, carboxyl, and amide groups. Glucose, galactose, and mannose were detected from NMR spectra whilst the occurrence of arabinose, glucose, galactose,...
Source: International Journal of Biological Macromolecules - May 5, 2019 Category: Biochemistry Authors: Vinothini G, Latha S, Arulmozhi M, Dhanasekaran D Tags: Int J Biol Macromol Source Type: research

Purification and characterization of an exopolysaccharide produced by Lactobacillus plantarum HY isolated from home-made Sichuan Pickle.
Abstract In the present study, the exopolysaccharide (EPS) producing strain HY was isolated from home-made Sichuan Pickle and identified as Lactobacillus plantarum. The EPS was purified by ethanol precipitation, and fractionation by column chromatography, and the yield of purified HY EPS was 1.423 g/L. The molecular weight of the purified HY EPS was 9.549 × 104 Da and composed of mannose, galactose, glucuronic acid and glucose in a mass percentage of 72.99%, 17.27%, 6.99% and 2.75%, respectively. FT-IR spectrum exhibited the existence of carboxyl and hydroxyl group. Partial sugar residues and glycosi...
Source: International Journal of Biological Macromolecules - May 4, 2019 Category: Biochemistry Authors: Liu T, Zhou K, Yin S, Liu S, Zhu Y, Yang Y, Wang C Tags: Int J Biol Macromol Source Type: research

Characterization and comparison of lignin derived from corncob residues to better understand its potential applications.
Abstract Lignocellulosic biomass is regarded as an ideal renewable resource for replacing traditional fossil fuels. Corncob residues (CRs) generated after hemicelluloses pre-extraction of corncobs were potential feedstocks to produce lignin-based products. Three lignin fractions, i.e., dioxane lignin (DL), milled-wood lignin (MWL), and enzymatic mild acidolysis lignin (EMAL), were isolated from CRs and characterized by HPLC, GPC, FT-IR, NMR, and TGA to reveal characteristics of CRs lignin to better understand its potential applications. The results showed that the yield of DL, MWL and EMAL were 12.8%, 14.7% and 15...
Source: International Journal of Biological Macromolecules - May 4, 2019 Category: Biochemistry Authors: Wang Y, Liu W, Zhang L, Hou Q Tags: Int J Biol Macromol Source Type: research

Natural polysaccharide-based smart (temperature sensing) and active (antibacterial, antioxidant and photoprotective) nanoparticles with potential application in biocompatible food coatings.
Abstract Smart and active nanoparticles are of increasing interest in food films and coatings application. In the current study, we purpose novel nanoparticles NPs-4(1:5) and NPs-4(1:5.5), which possess simultaneously both smart (temperature sensitive) and active (antibacterial, light absorbing and antioxidant) properties. The obtained nanoparticles are based on PEG/MC core with anthocyanidin and sodium acetate, and chitosan/gallotannin-based shell. The nanoparticles have hydrodynamic diameter ca. 450 nm and are positively charged (ζ-potential is 21 mV for NPs-4(1:5) and +23 mV for NPs-4(1:5.5). NPs-4(1...
Source: International Journal of Biological Macromolecules - May 4, 2019 Category: Biochemistry Authors: Kritchenkov AS, Egorov AR, Dubashynskaya NV, Volkova OV, Zabodalova LA, Suchkova EP, Kurliuk AV, Shakola TV, Dysin AP Tags: Int J Biol Macromol Source Type: research

Mesenchymal stem cells-curcumin loaded chitosan nanoparticles hybrid vectors for tumor-tropic therapy.
In this study, cell-nanoparticle hybrid vector was constructed using mesenchymal stem cells as the targeting cellular carrier and biotinylated chitosan polymer nanoparticles as the drug depot. Drug-loaded nanoparticles (hydrodynamic size =377.0 ± 14.6 nm and zeta potential = 9.6 ± 1.9 mV) were prepared by encapsulating hydrophobic model drug curcumin into biotinylated chitosan polymer. The biotin-modified nanoparticles were anchored on biotinylated mesenchymal stem cells surface by biotin-avidin binding, achieving an upload of 54.73 ± 3.95 pg/cell. The anchorage of nanoparticles o...
Source: International Journal of Biological Macromolecules - May 4, 2019 Category: Biochemistry Authors: Xu M, Asghar S, Dai S, Wang Y, Feng S, Jin L, Shao F, Xiao Y Tags: Int J Biol Macromol Source Type: research

Characterization of a chitin-glucan complex from the fruiting body of Termitomyces albuminosus (Berk.) Heim.
This study suggested that CGC from T. albuminosus was promising to be an alternative source of crustacean chitinous products in the industry of food, medicine, waste water treatment and so on in the future. PMID: 31063786 [PubMed - as supplied by publisher] (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 4, 2019 Category: Biochemistry Authors: Hong Y, Ying T Tags: Int J Biol Macromol Source Type: research

Structure, thermal stability and suspension rheological properties of alcoholic-alkaline-treated waxy rice starch.
Abstract In this work, the effect of alcoholic-alkaline treatment on the characteristics of waxy rice starch (WRS) and its aqueous suspensions was studied for the first time. Microscopy shows that the alcoholic-alkaline-treated WRS (AAT-WRS) was in the form of particles of lengths between 10 and 50 μm. Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) analyses indicate that the original A-type crystallites of WRS had been completely eliminated by the alcoholic-alkaline treatment and no new crystalline structure was formed. Fourier-transform infrared (FTIR) spectroscopy confirms the absence o...
Source: International Journal of Biological Macromolecules - May 4, 2019 Category: Biochemistry Authors: Chen F, Xie F, Liu P, Chen P Tags: Int J Biol Macromol Source Type: research

Evaluating the effect of aminoglycosides on the interaction between bovine serum albumins by atomic force microscopy.
In this study, the effect of aminoglycosides (AGs: streptomycin, gentamycin, lincomycin and clindamycin) on interactions between bovine serum albumin (BSA) was evaluated employing imaging and probing adhesion event by AFM. Multi-spectroscopy and molecular docking were supplementary to investigate the acting forces of the effect. AFM measurements revealed the aggregation of BSA grains and changes of adhesion forces at single molecule level. With adhesion forces between BSA pairs decomposed by Poisson method, specific forces in streptomycin, gentamycin, lincomycin and climdamycin were obviously decreased with the rate of 33....
Source: International Journal of Biological Macromolecules - May 4, 2019 Category: Biochemistry Authors: Wang Y, Wang J, Huang S, Liu C, Fu Y Tags: Int J Biol Macromol Source Type: research

A molecular investigation into the interaction of SiO2 nanoparticles with elastase by multispectroscopic techniques and kinetic studies.
Abstract The increasing use of nanoparticles in various industries has triggered the need to study their interconnection with biological macromolecules. The goal of this study was to survey the SiO2-nanoparticles efficacy on the thermal denaturation, conformation and activity of elastase at three temperatures of 303, 313 and 323 K in the Tris buffer at pH of 8.5. In this work, distinct techniques such as UV-vis Spectrophotometry, Spectrofluorometry, and circular dichroism were employed. The fluorescence studies showed that SiO2 nanoparticles extremely reduced the intensity of elastase with the static mechanism. ...
Source: International Journal of Biological Macromolecules - May 3, 2019 Category: Biochemistry Authors: Sadeghi-Kaji S, Shareghi B, Saboury AA, Farhadian S, Hemmati R Tags: Int J Biol Macromol Source Type: research

Hylocereus polyrhizus peel's high-methoxyl pectin: A potential source of hypolipidemic agent.
Abstract In the present study, high-methoxyl pectin (HMP) was extracted from Hylocereus polyrhizus peel's using physico-chemical process. In addition, the hypolipidemic activity of HMP was investigated at different concentration and time corresponding to its adsorption ability. FTIR and contact angle analysis were used to determine the sorbent characterization. A high degree of esterification (63.8%) and the contact angle (95.5°) confirmed hydrophobic nature and resulting bad wetting of the HMP extract, respectively. The methoxyl content in the pectin acted as an affinity-precursor of the pectin towards choles...
Source: International Journal of Biological Macromolecules - May 3, 2019 Category: Biochemistry Authors: Zaid RM, Mishra P, Wahid ZA, Sakinah AMM Tags: Int J Biol Macromol Source Type: research

The transcriptome analysis of the bamboo grasshopper provides insights into hypothermic stress acclimation.
In this study, high-throughput sequencing was used to analyze the transcriptome of C. kiangsu. Approximately 129,314,084 reads were generated using an Illumina sequencing. De novo assembly yielded 39,013 unigenes with an average length of 987 bp. Based on sequence similarity searches with known proteins, a total of 19,769 (50.67%) unigenes were identified. Of these annotated unigenes, 2114 and 11,412 unigenes were assigned to clusters of orthologous groups and gene ontology, respectively. Furthermore, 2128 simple sequence repeats (SSRs) were identified in the unigenes Differences were observed in gene expression after hy...
Source: International Journal of Biological Macromolecules - May 3, 2019 Category: Biochemistry Authors: Li R, Wang YT, Jiang GF Tags: Int J Biol Macromol Source Type: research

Therapeutic potential of cyanobacterial pigment protein phycoerythrin: in silico and in vitro study of BACE1 interaction and in vivo A β reduction.
Therapeutic potential of cyanobacterial pigment protein phycoerythrin: in silico and in vitro study of BACE1 interaction and in vivo Aβ reduction. Int J Biol Macromol. 2019 May 03;: Authors: Chaubey MG, Patel SNK, Rastogi RP, Srivastava PL, Singh AK, Madamwar D, Singh NK Abstract Cyanobacteria are an immense source of innovative classes of pharmacologically active compounds exhibiting various biological activities ranging from antioxidants, antibiotics, anticancer, anti-inflammatory to anti-Alzheimer's disease. In the present study, we primarily targeted the inhibition of Beta-site amyloid precur...
Source: International Journal of Biological Macromolecules - May 3, 2019 Category: Biochemistry Authors: Chaubey MG, Patel SNK, Rastogi RP, Srivastava PL, Singh AK, Madamwar D, Singh NK Tags: Int J Biol Macromol Source Type: research

Whole genome sequence of moderate halophilic marine bacterium Marinobacter litoralis SW-45: Abundance of non-coding RNAs.
Abstract A report on the de novo Whole Genome Sequence (WGS) of Marinobacter litoralis SW-45, a moderately salt-tolerant bacterium isolated from the seawater in Malaysia is presented. The strain has a genome size of 3.45 Mb and is capable of producing halophilic lipase, protease and esterase enzymes. Computational prediction of non-coding RNA (ncRNA) genes in M. litoralis SW-45 was performed using standalone software known as the non-coding RNA characterization (nocoRNAc). In addition, a phylogenetic tree showing the evolutionary relationship between the strain and other members of the genus Marinobacter was con...
Source: International Journal of Biological Macromolecules - May 2, 2019 Category: Biochemistry Authors: Musa H, Kasim FH, Gunny AAN, Gopinath SCB, Chinni SV, Ahmad MA Tags: Int J Biol Macromol Source Type: research

Salt-containing aqueous two-phase system shows predictable partition of proteins with surface amino acids residues.
In this study, we demonstrate that the partition of proteins in salt-containing ATPS (SATPS) depends only on the relation between the protein-surface amino acids and the type of salt using SATPS. The partition coefficients of four proteins changed depending on the kind of salt, according to the Hofmeister series. Interestingly, the partition coefficients of the proteins correlated to those of the combination of the amino acids in the surface of the protein with the correlation coefficients of>0.9. The results suggest that the interaction between the protein surface and aqueous ions plays an indispensable role for the pa...
Source: International Journal of Biological Macromolecules - May 2, 2019 Category: Biochemistry Authors: Shibata C, Iwashita K, Shiraki K Tags: Int J Biol Macromol Source Type: research

Chitosan sponges as a sustained release carrier system for the prophylaxis of orthopedic implant-associated infections.
Abstract The present investigation aims to study the chitosan sponge as a carrier matrix for the sustained antibiotic release system for the prophylaxis of orthopedic implant-associated infections (OIAIs). We have prepared sponges of three broad-spectrum antibiotics, namely vancomycin, ciprofloxacin and cefuroxime possessing different physicochemical properties. The blank, vancomycin, ciprofloxacin and cefuroxime loaded chitosan sponges were denoted as Blank-CH, CH-VAN, CH-CIP, and CH-CEF sponges. Chitosan sponges were assessed for morphology, drug-release, antibacterial potential, and preclinical evaluation using...
Source: International Journal of Biological Macromolecules - May 2, 2019 Category: Biochemistry Authors: Pawar V, Bulbake U, Khan W, Srivastava R Tags: Int J Biol Macromol Source Type: research

A promising enzyme anchoring probe for selective ethanol sensing in beverages.
Abstract A newly designed amperometric biosensor for the determination of ethanol through one-step electrochemical coating of (4,7-di(thiophen-2-yl)benzo[c][1,2,5]selenadiazole-co-1H-pyrrole-3-carboxylic acid) (TBeSe-co-P3CA) on a graphite electrode is presented. It was aimed to propose a newly synthesized copolymer with enhanced biosensing properties as a novel sensor for the quantification of ethanol. The conjugated copolymer (TBeSe-co-P3CA) was prepared through electrochemical polymerization by potential cycling. After polymer modification, alcohol oxidase (AOx) was immobilized on a modified electrode surface f...
Source: International Journal of Biological Macromolecules - May 2, 2019 Category: Biochemistry Authors: Soylemez S, Goker S, Toppare L Tags: Int J Biol Macromol Source Type: research

Polysaccharide from Spirulina platensis ameliorates diphenoxylate-induced constipation symptoms in mice.
This study found that the average molecular weight of PSP was 29, 600 Da, and mainly monosaccharides of PSP were rhamnose (24.7%), glucose (16.15%) and galactose (13.32%). The beneficial effects of PSP treatment include defecation improvement, increase of AchE activity, reduction of NO concentration, renovation of the damaged intestinal villus and affection on the expression of some related genes in the constipated mice. In addition, PSP had significant effects on the gut microbiota, showing the enhancement in abundance of beneficial bacteria including Akkermansia, Lactobacillus, Butyricimonas, Candidatus Arthromitus and...
Source: International Journal of Biological Macromolecules - May 1, 2019 Category: Biochemistry Authors: Ma H, Xiong H, Zhu X, Ji C, Xue J, Li R, Ge B, Cui H Tags: Int J Biol Macromol Source Type: research

Lectin from Dioclea violacea induces autophagy in U87 glioma cells.
Abstract The antitumor activity of DVL, a lectin purified from Dioclea violacea seeds, on the U87 human glioma cell line was evaluated and compared with Canavalia ensiformis lectin (ConA). Treatment with DVL (10-100 μg/mL; 24-96 h) induced alterations in cell morphology, decreased cell numbers and clonogenic survival in a time- and concentration-dependent manner. DVL caused significant decreases in cell viability and impaired cell migration. Mechanistically, DVL treatment (12 h) disrupted mitochondrial electrochemical gradient, without ROS accumulation or caspase activation. In the absence of apoptosis, D...
Source: International Journal of Biological Macromolecules - May 1, 2019 Category: Biochemistry Authors: Nascimento APM, Wolin IAV, Welter PG, Heinrich IA, Zanotto-Filho A, Osterne VJS, Lossio CF, Silva MTL, Nascimento KS, Cavada BS, Leal RB Tags: Int J Biol Macromol Source Type: research