C-terminal region of Rv1039c (PPE15) protein of Mycobacterium tuberculosis targets host mitochondria to induce macrophage apoptosis
This study investigated the role of C-terminal region of Rv1039c (PPE15) in inducing mitochondrial perturbations and macrophage apoptosis. Ourin-silico studies revealed the disordered, coiled, and hydrophobic C-terminal region in Rv1039c has similarity with C-terminal of mitochondria-targeting pro-apoptotic host proteins. Wild type Rv1039c and C-terminal deleted Rv1039c (Rv1039c-/-Cterm) recombinant proteins were purified and theirM. smegmatis knock-in strains were constructed which were used forin-vitro experiments. Confocal microscopy showed localization of Rv1039c to mitochondria of PMA-differentiated THP1 macrophages; ...
Source: Apoptosis - April 14, 2024 Category: Molecular Biology Source Type: research
Effects of Rifampicin Plus Levofloxacin or Isoniazid on Immune Function of Patients with Pulmonary Tuberculosis
CONCLUSIONS: Compared with rifampicin + isoniazid, rifampicin + levofloxacin had a better clinical effect in the treatment of pulmonary TB and could effectively regulate patients' immune functions and inhibit inflammatory reactions. The current research team recommends that rifampicin + levofloxacin become the preferred treatment for pulmonary TB in the absence of a drug allergy.PMID:38607224 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - April 12, 2024 Category: Complementary Medicine Authors: Feng Lv Nan Tang Source Type: research
Effects of Rifampicin Plus Levofloxacin or Isoniazid on Immune Function of Patients with Pulmonary Tuberculosis
CONCLUSIONS: Compared with rifampicin + isoniazid, rifampicin + levofloxacin had a better clinical effect in the treatment of pulmonary TB and could effectively regulate patients' immune functions and inhibit inflammatory reactions. The current research team recommends that rifampicin + levofloxacin become the preferred treatment for pulmonary TB in the absence of a drug allergy.PMID:38607224 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - April 12, 2024 Category: Complementary Medicine Authors: Feng Lv Nan Tang Source Type: research
Relapse in ocular tuberculosis: relapse rate, risk factors and clinical management in a non-endemic country
CONCLUSIONS: Our results suggest that approximately one-third of patients with OTB will experience relapse after achieving clinical inactivity. The initial disease course and poor response to treatment predict the likelihood of relapse in the long-term follow-up. Topical corticosteroids were particularly effective in relapse presenting as anterior uveitis.PMID:38609164 | DOI:10.1136/bjo-2024-325207 (Source: The British Journal of Ophthalmology)
Source: The British Journal of Ophthalmology - April 12, 2024 Category: Opthalmology Authors: Ikhwanuliman Putera Josianne C E M Ten Berge Alberta A H J Thiadens Willem A Dik Rupesh Agrawal P Martin van Hagen Rina La Distia Nora Saskia M Rombach Source Type: research
Effects of Rifampicin Plus Levofloxacin or Isoniazid on Immune Function of Patients with Pulmonary Tuberculosis
CONCLUSIONS: Compared with rifampicin + isoniazid, rifampicin + levofloxacin had a better clinical effect in the treatment of pulmonary TB and could effectively regulate patients' immune functions and inhibit inflammatory reactions. The current research team recommends that rifampicin + levofloxacin become the preferred treatment for pulmonary TB in the absence of a drug allergy.PMID:38607224 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - April 12, 2024 Category: Complementary Medicine Authors: Feng Lv Nan Tang Source Type: research
Design of a tunable bacterial gene expression system using engineered σ factors
Appl Environ Microbiol. 2024 Apr 12:e0002124. doi: 10.1128/aem.00021-24. Online ahead of print.ABSTRACTExtracytoplasmic function (ECF) σ factors selectively upregulate expression of specific genes in bacteria. These σ factors, belonging to the σ70 family, are much smaller than the primary, housekeeping σ factor with two helical domains that interact with the Pribnow box and the -35 element of the promoter DNA. Structural studies reveal that promoter specificity in a σ factor is determined by the interactions between a loop (L3) and the Pribnow box element. Similarly, the efficiency of transcription initiation is gover...
Source: Applied and Environmental Microbiology - April 12, 2024 Category: Microbiology Authors: Twinkal Patel Amit Dinda Sankar Mahesh Savitha Nadig Nishank Reddy Balasubramanian Gopal Source Type: research
Endobronchial growth: Tumor or tuberculosis
J Family Med Prim Care. 2024 Feb;13(2):792-796. doi: 10.4103/jfmpc.jfmpc_1204_23. Epub 2024 Mar 6.ABSTRACTEndobronchial tuberculosis (EBTB) is characterized by tuberculosis infection of the tracheobronchial tree. It has variable presentation but tumorous growth-like presentation in bronchus is very rare. The clinical and radiological features are non-specific, which creates a diagnostic dilemma. Bronchoscopy and biopsy of the lesion are mandatory to confirm the diagnosis. In this case series, we are presenting three unique cases of endobronchial growth diagnosed as EBTB after biopsy and evaluation of bronchoalveolar lavage...
Source: Primary Care - April 12, 2024 Category: Primary Care Authors: Zuber Ahmad Imrana Masood Ummul Baneen Saima Ejaz Suhailur Rehman Source Type: research
Community-based Advocacy Communication Social Mobilization (ACSM) intervention by empowering key community leaders: Evaluation using the RE-AIM framework
CONCLUSION: This study was community led and involved leaders from diverse backgrounds, optimizing the chance of success. For sustainability, we have initiated TB support group.PMID:38605780 | PMC:PMC11006061 | DOI:10.4103/jfmpc.jfmpc_772_23 (Source: Primary Care)
Source: Primary Care - April 12, 2024 Category: Primary Care Authors: Reenaa Mohan Ganapathy Kalaiselvan Source Type: research
Antitubercular drug-induced lichen planus: A case study with a mini literature review
CONCLUSION: Lichenoid drug eruptions are characterized by type IV hypersensitivity reactions, and rechallenge is required to ensure safer treatment since the risk of disseminated and multi-drug-resistant tuberculosis increases with the cessation of antitubercular therapy.PMID:38605770 | PMC:PMC11006073 | DOI:10.4103/jfmpc.jfmpc_499_23 (Source: Primary Care)
Source: Primary Care - April 12, 2024 Category: Primary Care Authors: Sanjana C Shanmukhappa Winny John Vineeta Kevalramani Srivatsa Lokeshwaran Shireen Furtado Mahesh Nosenoor Source Type: research
Relapse in ocular tuberculosis: relapse rate, risk factors and clinical management in a non-endemic country
CONCLUSIONS: Our results suggest that approximately one-third of patients with OTB will experience relapse after achieving clinical inactivity. The initial disease course and poor response to treatment predict the likelihood of relapse in the long-term follow-up. Topical corticosteroids were particularly effective in relapse presenting as anterior uveitis.PMID:38609164 | DOI:10.1136/bjo-2024-325207 (Source: The British Journal of Ophthalmology)
Source: The British Journal of Ophthalmology - April 12, 2024 Category: Opthalmology Authors: Ikhwanuliman Putera Josianne C E M Ten Berge Alberta A H J Thiadens Willem A Dik Rupesh Agrawal P Martin van Hagen Rina La Distia Nora Saskia M Rombach Source Type: research
Design of a tunable bacterial gene expression system using engineered σ factors
Appl Environ Microbiol. 2024 Apr 12:e0002124. doi: 10.1128/aem.00021-24. Online ahead of print.ABSTRACTExtracytoplasmic function (ECF) σ factors selectively upregulate expression of specific genes in bacteria. These σ factors, belonging to the σ70 family, are much smaller than the primary, housekeeping σ factor with two helical domains that interact with the Pribnow box and the -35 element of the promoter DNA. Structural studies reveal that promoter specificity in a σ factor is determined by the interactions between a loop (L3) and the Pribnow box element. Similarly, the efficiency of transcription initiation is gover...
Source: Applied and Environmental Microbiology - April 12, 2024 Category: Microbiology Authors: Twinkal Patel Amit Dinda Sankar Mahesh Savitha Nadig Nishank Reddy Balasubramanian Gopal Source Type: research
Management of cutaneous tuberculosis in hand - Rare and disabling: A case report
CONCLUSION: Long-term complications of untreated CTB encompass significant contractures, scarring, and the onset of carcinomas and sarcomas. Prompt diagnosis facilitates timely and effective treatment, averting these sequelae and yielding high patient satisfaction.PMID:38608519 | DOI:10.1016/j.ijscr.2024.109631 (Source: Hand Surgery)
Source: Hand Surgery - April 12, 2024 Category: Surgery Authors: Hardisiswo Soedjana Betha Egih Riestiano Lisa Y Hasibuan Selvy Harianti Source Type: research
Antitubercular drug-induced lichen planus: A case study with a mini literature review
CONCLUSION: Lichenoid drug eruptions are characterized by type IV hypersensitivity reactions, and rechallenge is required to ensure safer treatment since the risk of disseminated and multi-drug-resistant tuberculosis increases with the cessation of antitubercular therapy.PMID:38605770 | PMC:PMC11006073 | DOI:10.4103/jfmpc.jfmpc_499_23 (Source: Primary Care)
Source: Primary Care - April 12, 2024 Category: Primary Care Authors: Sanjana C Shanmukhappa Winny John Vineeta Kevalramani Srivatsa Lokeshwaran Shireen Furtado Mahesh Nosenoor Source Type: research
Community-based Advocacy Communication Social Mobilization (ACSM) intervention by empowering key community leaders: Evaluation using the RE-AIM framework
CONCLUSION: This study was community led and involved leaders from diverse backgrounds, optimizing the chance of success. For sustainability, we have initiated TB support group.PMID:38605780 | PMC:PMC11006061 | DOI:10.4103/jfmpc.jfmpc_772_23 (Source: Primary Care)
Source: Primary Care - April 12, 2024 Category: Primary Care Authors: Reenaa Mohan Ganapathy Kalaiselvan Source Type: research
