Tobacco smoking and acute exercise on immune-inflammatory responses among relative short and longer smoking histories.
This study examined the acute effects of combined tobacco smoking and exercise on immune-inflammatory responses in smoker populations with shorter or longer smoking history. The cohort comprised 14 young male adult (YSM) and 12 middle-aged (MSM) male active cigarette smokers matched for aerobic fitness and smoking behavior. Following an initial familiarization and baseline testing session, participants completed a smoking and exercise protocol. This protocol involved the inhalation of two cigarettes (12 mg tar, 1 mg nicotine) within 15 min, and following a 10 min recovery period, 40 min of cycling at 50% peak aer...
Source: Cytokine - June 19, 2019 Category: Molecular Biology Authors: Hartmann T, Marino F, Duffield R Tags: Cytokine Source Type: research
Statins influence biomarkers of low grade inflammation in apparently healthy people or patients with chronic diseases: A systematic review and meta-analysis of randomized clinical trials.
CONCLUSIONS: In conclusion, we found that statins administration in apparently healthy people or those with chronic diseases help reducing serum CRP concentrations. In addition, Atorvastatin administration resulted in reduced serum IL-6 concentrations in these people. PMID: 31228727 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - June 19, 2019 Category: Molecular Biology Authors: Milajerdi A, Larijani B, Esmaillzadeh A Tags: Cytokine Source Type: research
Anti-inflammatory cytokines in autism spectrum disorders: A systematic review and meta-analysis.
CONCLUSIONS: This meta-analysis provides evidence for the lower concentration of anti-inflammatory cytokines IL-10 and IL-1Ra in autistic patients compared with control subjects. Also, meta-regression analyses point to the interaction of latitude, age, and gender with peripheral alterations of associated anti-inflammatory cytokines. PMID: 31228728 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - June 19, 2019 Category: Molecular Biology Authors: Saghazadeh A, Ataeinia B, Keynejad K, Abdolalizadeh A, Hirbod-Mobarakeh A, Rezaei N Tags: Cytokine Source Type: research
Taurine supplementation increases irisin levels after high intensity physical training in obese women.
Abstract BACKGROUND: Irisin is a myokine/adipokine that under stimulus of physical exercise is able to improve thermogenic capacity in adipose tissue. Likewise, taurine supplementation has demonstrated similar effects on energy metabolism. Therefore, we hypothesized that taurine supplementation combined with physical training may induce an increase in irisin concentrations, optimizing energy metabolism in obese individuals. OBJECTIVE: To evaluate if taurine supplementation associated with a high intensity physical training program increases irisin levels in obese women. METHODS: double-blind study with 2...
Source: Cytokine - June 18, 2019 Category: Molecular Biology Authors: Batitucci G, Brandao CFC, De Carvalho FG, Marchini JS, Pfrimer K, Ferrioli E, Cunha F, Papoti M, Terrazas SIBM, Junqueira-Franco MVM, da Silva ASR, Freitas EC Tags: Cytokine Source Type: research
Changes in inflammatory protein and lipid mediator profiles persist after antitubercular treatment of pulmonary and extrapulmonary tuberculosis: A prospective cohort study.
CONCLUSION: Our findings describe systemic and durable changes in the concentrations of inflammatory cytokines and lipid mediators in both PTB and EPTB and emphasize the role of M. tuberculosis bacterial burden and site of disease in modulating patient immune biomarkers. PMID: 31226436 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - June 18, 2019 Category: Molecular Biology Authors: Vinhaes CL, Oliveira-de-Souza D, Silveira-Mattos PS, Nogueira B, Shi R, Wei W, Yuan X, Zhang G, Cai Y, Barry CE, Via LE, Fukutani KF, Andrade BB, Mayer-Barber KD Tags: Cytokine Source Type: research
An underestimated pathogen: Staphylococcus epidermidis induces pro-inflammatory responses in human alveolar epithelial cells.
This study addressed the pro-inflammatory capacity of different S. epidermidis strains on human alveolar epithelial cells. METHODS: A549 cell monolayers were stimulated by live bacteria of S. epidermidis RP62A strain (biofilm-positive) and ATCC 12228 strain (biofilm-negative) at a multiplicity of infection ratio of 10 for 24 h. LPS (100 ng/ml) and Pam3CSK4 (1 µg/ml) were used for comparisons. Cell viability was measured by MTT method. The mRNA and protein expression of inflammatory mediators and toll-like receptor (TLR)-2 were assessed using RT-PCR, immunoassays and immunofluorescence. RESULTS: Both S...
Source: Cytokine - June 18, 2019 Category: Molecular Biology Authors: Dong Y, Glaser K, Schlegel N, Claus H, Speer CP Tags: Cytokine Source Type: research
Short-term RANKL exposure initiates a neoplastic transcriptional program in the basal epithelium of the murine salivary gland.
Abstract Although salivary gland cancers comprise only ∼3-6% of head and neck cancers, treatment options for patients with advanced-stage disease are limited. Because of their rarity, salivary gland malignancies are understudied compared to other exocrine tissue cancers. The comparative lack of progress in this cancer field is particularly evident when it comes to our incomplete understanding of the key molecular signals that are causal for the development and/or progression of salivary gland cancers. Using a novel conditional transgenic mouse (K5:RANKL), we demonstrate that Receptor Activator of NFkB Ligand (...
Source: Cytokine - June 18, 2019 Category: Molecular Biology Authors: Hai L, Szwarc MM, Lonard DM, Rajapakshe K, Perera D, Coarfa C, Ittmann M, Fernandez-Valdivia R, Lydon JP Tags: Cytokine Source Type: research
In vivo and in vitro anti-inflammatory and pro-osteogenic effects of citrus cystatin CsinCPI-2.
li JA Abstract Cystatins are natural inhibitors of cysteine peptidases. Recently, cystatins derived from plants, named phytocystatins, have been extensively studied. Among them, CsinCPI-2 proteins from Citrus sinensis were identified and recombinantly produced by our group. Thus, this study described the recombinant expression, purification, and inhibitory activity of this new phytocystatin against human cathepsins K and B and assessed the anti-inflammatory effect of CsinCPI-2 in vitro in mouse and in vivo in rats. In addition, the pro-osteogenic effect of CsinCPI-2 was investigated in vitro. The inflammatory resp...
Source: Cytokine - June 18, 2019 Category: Molecular Biology Authors: Leguizamón NDP, Rodrigues EM, de Campos ML, Nogueira AVB, Viola KS, Schneider VK, Neo-Justino DM, Tanomaru-Filho M, Zambuzzi WF, Henrique-Silva F, Soares-Costa A, Faria G, Cirelli JA Tags: Cytokine Source Type: research
Does Prolactin treatment trigger imunoendocrine alterations during experimental T. cruzi infection?
In this study, we demonstrated that PRL can influence many aspects of the immune response during the experimental Chagas' disease, and this substance could be used as a supporting trial along with the conventional drug treatment. PMID: 31163343 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - June 1, 2019 Category: Molecular Biology Authors: Del Vecchio Filipin M, Brazão V, Santello FH, da Costa CMB, Paula Alonso Toldo M, Rossetto de Morais F, do Prado Júnior JC Tags: Cytokine Source Type: research
Induced and spontaneous colitis mouse models reveal complex interactions between IL-10 and IL-12/IL-23 pathways.
Abstract Crohn's disease (CD) and ulcerative colitis (UC) are the two major forms of inflammatory bowel disease (IBD). These idiopathic and chronic diseases result from inflammation of the gastrointestinal tract and are mainly mediated by the immune system. Genome wide association studies link genes of the IL-12 and IL-23 biology to both CD and UC susceptibility. IL-12 and IL-23 cytokines share a functional subunit, p40, and their respective receptors also share a functional subunit, IL-12Rβ1. However, clinical trials targeting p40, and thus inhibiting both IL-12 and IL-23 pathways, provided mitigated effects...
Source: Cytokine - May 31, 2019 Category: Molecular Biology Authors: Hurtubise R, Audiger C, Dominguez-Punaro MC, Chabot-Roy G, Chognard G, Raymond-Marchand L, Coderre L, Chemtob S, Michnick SW, Rioux JD, Lesage S Tags: Cytokine Source Type: research
Ureaplasma species modulate cell adhesion molecules and growth factors in human brain microvascular endothelial cells.
This study addressed mRNA and protein expression of intercellular and vascular cell adhesion molecules (ICAM-1, VCAM-1), granulocyte-colony stimulating factor (G-CSF), and vascular endothelial growth factor (VEGF) in native or lipopolysaccharide (LPS) co-stimulated human brain microvascular endothelial cells (HBMEC) exposed to Ureaplasma (U.) urealyticum or U. parvum. Ureaplasma spp. reduced G-CSF mRNA (p
Source: Cytokine - May 31, 2019 Category: Molecular Biology Authors: Silwedel C, Speer CP, Haarmann A, Fehrholz M, Claus H, Schlegel N, Glaser K Tags: Cytokine Source Type: research
Leptin as a predictive marker for metabolic syndrome.
Abstract Metabolic syndrome poses a major threat on human health affecting the quality of life. Adipose tissue is an important organ which plays a crucial role in the pathogenesis of metabolic syndrome. Adipocytokines secreted by the adipose tissue plays a critical role in storage, food intake, energy expenditure, lipid and glucose metabolism. Leptin is primarily involved in regulating food intake, body weight and energy homeostasis through neuroendocrine functions. Contemporary research suggests that leptin also influences insulin sensitivity and lipid metabolism. High leptin concentrations are directly associate...
Source: Cytokine - May 30, 2019 Category: Molecular Biology Authors: Ghadge AA, Khaire AA Tags: Cytokine Source Type: research
Serum soluble ST2 as a marker of renal scar in pediatric upper urinary tract infection.
CONCLUSION: These results suggest that serum soluble ST2 levels on admission could be a useful biomarker of subsequent renal scarring in pediatric patients with upper urinary tract infection. PMID: 31153005 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - May 29, 2019 Category: Molecular Biology Authors: Ohta N, Yasudo H, Mizutani M, Matsushige T, Fukano R, Kittaka S, Maehara K, Ichihara K, Ohga S, Hasegawa S Tags: Cytokine Source Type: research
TGF- β induces PML SUMOylation, degradation and PML nuclear body disruption.
TGF-β induces PML SUMOylation, degradation and PML nuclear body disruption. Cytokine. 2019 May 29;120:264-272 Authors: El-Asmi F, El-Mchichi B, Maroui MA, Dianoux L, Chelbi-Alix MK Abstract ProMyelocytic Leukemia (PML) protein is essential for the formation of nuclear matrix-associated organelles named PML nuclear bodies (NBs) that act as a platform for post-translational modifications and protein degradation. PML NBs harbor transiently and permanently localized proteins and are associated with the regulation of several cellular functions including apoptosis. There are seven PML isoforms, six nuc...
Source: Cytokine - May 29, 2019 Category: Molecular Biology Authors: El-Asmi F, El-Mchichi B, Maroui MA, Dianoux L, Chelbi-Alix MK Tags: Cytokine Source Type: research
Rab7b participation on the TLR4 (Toll-like receptor) endocytic pathway in Shiga toxin-associated Hemolytic Uremic Syndrome (HUS).
CONCLUSION: The colocalization of TLR4 and Rab7b allows us to suggest Rab7b participation in the control of the TLR4 endocytic pathway in HUS patient monocytes. A consequential fall in cytokine production throughout the early follow up of HUS is demonstrated. PMID: 31153054 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - May 29, 2019 Category: Molecular Biology Authors: Lafalla Manzano AF, Gil Lorenzo AF, Bocanegra V, Costantino VV, Cacciamani V, Benardon ME, Vallés PG Tags: Cytokine Source Type: research
Endothelial injury is closely related to osteopontin and TNF receptor-mediated inflammation in end-stage renal disease.
CONCLUSION: Among novel renal and cardiovascular biomarkers, OPN and TNFR2 are closely related to sTM. This may link endothelial damage, vascular remodeling and inflammation. Progression of RAC parallels a presumed compensatory rise in sTM, reflecting endothelial injury. sTM has an intricate role in endothelial function and potential clinical and prognostic applications. PMID: 31153055 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - May 29, 2019 Category: Molecular Biology Authors: Batko K, Krzanowski M, Gajda M, Dumnicka P, Fedak D, Woziwodzka K, Sułowicz W, Kuźniewski M, Litwin JA, Krzanowska K Tags: Cytokine Source Type: research
Induction effects of Faecalibacterium prausnitzii and its extracellular vesicles on toll-like receptor signaling pathway gene expression and cytokine level in human intestinal epithelial cells.
In this study, Caco-2 cell line was treated with F. prausitzii and its EVs. Using the protein levels of 12 cytokines were also evaluated by ELISA assay. F. prausnitzii induced upregulation in FOS, JUN, TNF-α, NFKB1, TLR3, IKBKB and CD86 genes. Furthermore, stimulation of Caco-2 cells with EVs derived from F. prausnitzii induced upregulation of CXCL8, CCL2, FOS, MAP2K4, TLR7, TLR3, IRF1, NFKBIA and TNF-α genes. Based on ELISA assay, Caco-2 cells treated with F. prausnitzii and its EVs showed a significant increase in TNF-α, IL-4, IL-8, and IL-10 expression and significant decreased in IL-1, IL-2, IL-6, IL-...
Source: Cytokine - May 29, 2019 Category: Molecular Biology Authors: Rabiei N, Ahmadi Badi S, Ettehad Marvasti F, Nejad Sattari T, Vaziri F, Siadat SD Tags: Cytokine Source Type: research
Bone marrow mesenchymal stem cells from patients with SLE maintain an interferon signature during in vitro culture.
CONCLUSIONS: BMSCs from SLE patients are heterogeneous. A subgroup of SLE BMSC is distinguished from other SLE BMSC and from controls by increased levels of mRNAs induced by type I interferons. This subgroup of SLE patients had increased levels of IFNβ in vivo. PMID: 31153744 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - May 29, 2019 Category: Molecular Biology Authors: Gao L, OConnell M, Allen M, Liesveld J, McDavid A, Anolik JH, Looney RJ Tags: Cytokine Source Type: research
Hepatocyte growth control by SOCS1 and SOCS3.
Abstract The extraordinary capacity of the liver to regenerate following injury is dependent on coordinated and regulated actions of cytokines and growth factors. Whereas hepatocyte growth factor (HGF) and epidermal growth factor (EGF) are direct mitogens to hepatocytes, inflammatory cytokines such as TNFα and IL-6 also play essential roles in the liver regeneration process. These cytokines and growth factors activate different signaling pathways in a sequential manner to elicit hepatocyte proliferation. The kinetics and magnitude of these hepatocyte-activating stimuli are tightly regulated to ensure restora...
Source: Cytokine - May 29, 2019 Category: Molecular Biology Authors: Khan MGM, Ghosh A, Variya B, Santharam MA, Kandhi R, Ramanathan S, Ilangumaran S Tags: Cytokine Source Type: research
Chronic ethanol consumption increases reactive oxygen species generation and the synthesis of pro-inflammatory proteins in the heart through TNFR1-dependent mechanisms.
Abstract We evaluated the role of tumor necrosis factor (TNF)-α receptor 1 (TNFR1) on ethanol-induced cardiac dysfunction. Male C57BL/6J wild-type (WT) or TNFR1-deficient mice (TNFR1-/-) were treated with ethanol (20% v/v) for 10 weeks. Increased protein expression of TNFR1 and NFκB p65 was detected in the left ventricle (LV) of WT mice chronically treated with ethanol. Echocardiographic analysis showed that ethanol consumption increased left ventricular posterior wall end-diastolic diameter and left ventricular posterior wall end-systolic diameter in WT, but not TNFR1-/- mice. Increased levels of TN...
Source: Cytokine - May 28, 2019 Category: Molecular Biology Authors: Nakashima MA, Silva CBP, Gonzaga NA, Simplicio JA, Omoto ACM, Tirapelli LF, Tanus-Santos JE, Tirapelli CR Tags: Cytokine Source Type: research
Interleukin-12p40 variant form reduces Interleukin-12p80 secretion.
In this study, we found that activated mouse bone marrow-derived DCs (BMDCs) produced a p40 splice variant form mRNA in addition to the conventional p40 mRNA. This p40 variant mRNA was produced by alternative splicing in exon 5, and possessed a premature stop codon. As a result, the p40 variant protein contained 157 amino acids of the N-terminal part of p40 and an additional 10 novel amino acids. When the p40 variant was expressed in HEK-293T cells, it was not secreted from the cells. To investigate the function of the p40 variant, it was co-expressed with p40 and/or p35. The p40 variant did not affect the secretion of IL-...
Source: Cytokine - May 27, 2019 Category: Molecular Biology Authors: Oshikiri Y, Nara H, Takeda Y, Araki A, Nemoto N, Gazi MY, Saito S, Saitoh S, Nakajima O, Asao H Tags: Cytokine Source Type: research
IL-33/IL-33R in various types of carotid artery atherosclerotic lesions.
This study included 191 consecutive patients submitted for carotid endarterectomy (CEA). Preoperative serum levels of IL-33 and soluble ST2 (sST2) were measured. Atherosclerotic plaques obtained during surgery were initially histologically classified and immunohistochemical analyzes of IL-33, IL-33R, CD68 and alpha-SMA expression was performed. Ultrasound assessment of the level of carotid stenosis in each patient was performed prior to carotid surgery. Demographic and clinical data such as gender, age, smoking status, blood pressure, glycaemia, hemoglobin and creatinine levels, and comorbidities were collected and the com...
Source: Cytokine - May 24, 2019 Category: Molecular Biology Authors: Stankovic M, Ljujic B, Babic S, Maravic-Stojkovic V, Mitrovic S, Arsenijevic N, Radak D, Pejnovic N, Lukic ML Tags: Cytokine Source Type: research
Evaluation of IVIG response in relation to Th1/Th2 cytokines in pediatricm immune thrombocytopenia.
Abstract Immune thrombocytopenia (ITP) is a common immune-mediated bleeding disorder in children, and intravenous immunoglobulin (IVIG) is widely used as the initial therapy of ITP. Effective predictive factors of response to IVIG in ITP are important for guiding the treatment decisions. A retrospective study was performed on 197 Chinese ITP patients, and the data of their serum interleukin (IL)-2, IL-4, IL-6, IL-10, tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) levels, age at onset, duration of disease, white blood cell count (WBC), platelet count, and gender ratio were collected. ...
Source: Cytokine - May 23, 2019 Category: Molecular Biology Authors: Chen YY, Zhou YQ, Zhao N, Zhang Y, Xu WQ, Tang YM Tags: Cytokine Source Type: research
Coordination between innate immune cells, type I IFNs and IRF5 drives SLE pathogenesis.
Abstract Systemic lupus erythematosus (SLE) is a complex autoimmune disease which affects multiple organs. The type I interferon (IFN) gene signature and circulating autoantibodies are hallmarks of SLE. Plasmacytoid dendritic cells (pDCs) are considered the main producers of type I IFN and production is modulated by multiple other immune cell types. In SLE, essentially every immune cell type is dysregulated and aberrant deregulation is thought to be due, in part, to direct or indirect exposure to IFN. Genetic variants within or around the transcription factor interferon regulatory factor 5 (IRF5) associate with SL...
Source: Cytokine - May 23, 2019 Category: Molecular Biology Authors: Matta B, Barnes BJ Tags: Cytokine Source Type: research
Interleukin-10 and interleukin-1 receptor antagonist distinguish between patients with sepsis and the systemic inflammatory response syndrome (SIRS).
Abstract The current study evaluated the potential of clinical parameters and circulating biomarkers to distinguish sepsis from SIRS in patients admitted with systemic inflammation. Clinical parameters, leukocyte counts and platelets were measured on admission. Circulating C-reactive protein (CRP), procalcitonin (PCT) and cytokine concentrations were quantified using laser immunonephelometry, immunoluminescence and a Bio-Plex suspension bead array system respectively. Blood, sputum, urine, peritoneal and cerebrospinal fluid were sent for microscopy and culture. Based on clinical information and the results of micr...
Source: Cytokine - May 21, 2019 Category: Molecular Biology Authors: Potjo M, Theron AJ, Cockeran R, Sipholi NN, Steel HC, Bale TV, Meyer PWA, Anderson R, Tintinger GR Tags: Cytokine Source Type: research
Cytokine expression profiles in cervical mucus from patients with cervical cancer and its precursor lesions.
Abstract Human papillomavirus (HPV) infection can persist in the cervical epithelium without provoking a strong host immune response, leading to the development of cervical cancer. Cytokines, which mediate innate and adaptive immune activities, are secreted in the cervical mucus; however, there is currently no appropriate method for assessing cytokine levels in mucus specimens. Here, we employed multiplexed bead-based immunoassays to examine cytokine levels in cervical mucus using both weighted-volume and total protein concentration methods to adjust for different specimen volumes in individual patients. Out of 18...
Source: Cytokine - May 20, 2019 Category: Molecular Biology Authors: Otani S, Fujii T, Kukimoto I, Yamamoto N, Tsukamoto T, Ichikawa R, Nishio E, Iwata A Tags: Cytokine Source Type: research
Amino acid residues involved in the heparin-binding activity of murine IL-12 in the context of an antibody-cytokine fusion protein.
Penichet ML Abstract An antibody-cytokine fusion protein, composed of the murine single-chain cytokine interleukin-12 (IL-12) genetically fused to a human IgG3 specific for the human tumor-associated antigen HER2/neu maintains antigen binding, cytokine bioactivity, and IL-12 heparin-binding activity. This latter property is responsible for the binding of the cytokine to glycosaminoglycans (GAGs) on the cell surface and the extracellular matrix and has been implicated in modulating IL-12 bioactivity. Previous studies indicate that the p40 subunit of human and murine IL-12 is responsible for the heparin-binding acti...
Source: Cytokine - May 20, 2019 Category: Molecular Biology Authors: Luria-Pérez R, Candelaria PV, Daniels-Wells TR, Rodríguez JA, Helguera G, Penichet ML Tags: Cytokine Source Type: research
Restoring thymic function: Then and now.
Abstract Thymic vulnerability, a leading cause of defective immunity, was discovered decades ago. To date, several strategies have been investigated to unveil any immunorestorative capacities they might confer. Studies exploiting castration, transplantation, adoptive cell therapies, hormones/growth factors, and cytokines have demonstrated enhanced in vitro and in vivo thymopoiesis, albeit with clinical restrictions. In this review, we will dissect the thymus on a physiological and pathological level and discuss the pros and cons of several strategies esteemed thymotrophic from a pre-clinical perspective. Finally, ...
Source: Cytokine - May 17, 2019 Category: Molecular Biology Authors: El-Kadiry AE, Rafei M Tags: Cytokine Source Type: research
Inflammatory, oxidative stress and anti-oxidative markers in patients with endometrial carcinoma and diabetes.
In this study, we aimed to investigate inflammatory and oxidative stress markers in patients with diabetes (DM) and endometrial carcinoma (EC) separately and in combination. METHODS: In a case-control study design, a total of 88 participants were enrolled including: 37 patients with EC (19 with DM and 18 without DM), 29 with type2 diabetes and 22 healthy controls. Cancer patients were sampled before treatment. Serum oxidative stress markers including: oxidized low density lipoprotein (ox-LDL,) nitric oxide (NO), advanced glycation end-products (AGEs) and advanced oxidation protein products (AOPP), malondialdehyde (MDA...
Source: Cytokine - May 14, 2019 Category: Molecular Biology Authors: Heidari F, Rabizadeh S, Ali Mansournia M, Mirmiranpoor H, Salehi SS, Akhavan S, Esteghamati A, Nakhjavani M Tags: Cytokine Source Type: research
Is Granulocyte colony-stimulating factor associated with development of aortitis?
Abstract The aim of this study was to mention some methodological issues in a study which investigate the effect of Granulocyte colony-stimulating factor on developing of aortitis. PMID: 31100683 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - May 14, 2019 Category: Molecular Biology Authors: Bidhendi Yarandi R, Panahi MH Tags: Cytokine Source Type: research
Effects of continuous high-dose G-CSF administration on hematopoietic stem cell mobilization and telomere length in patients with amyotrophic lateral sclerosis - a pilot study.
In conclusion, our data show that continuous G-CSF treatment fails to increase circulating CD34+ HSPC in ALS patients. Cytokine profiles revealed G-CSF-mediated immunomodulatory and proteolytic effects. Interestingly, despite intense G-CSF stimulation, telomere length was not significantly shortened. PMID: 31100684 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - May 14, 2019 Category: Molecular Biology Authors: Iberl S, Meyer AL, Müller G, Peters S, Johannesen S, Kobor I, Beier F, Brümmendorf TH, Hart C, Schelker R, Herr W, Bogdahn U, Grassinger J Tags: Cytokine Source Type: research
Resistin induces breast cancer cells epithelial to mesenchymal transition (EMT) and stemness through both adenylyl cyclase-associated protein 1 (CAP1)-dependent and CAP1-independent mechanisms.
Abstract Breast cancer incidence and metastasis in postmenopausal women are known to associate with obesity, but the molecular mechanisms behind this association are largely unknown. We investigated the effect of adipokine resistin on epithelial to mesenchymal transition (EMT) and stemness in breast cancer cells in vitro. Previous reports demonstrated that the inflammatory actions of resistin are mediated by the adenylyl cyclase-associated protein 1 (CAP1), which serves as its receptor. As a model for our study, we used MCF-7 and MDA-MB-231 breast cancer and MCF-10A breast epithelial cells. We showed that in MCF-7...
Source: Cytokine - May 11, 2019 Category: Molecular Biology Authors: Avtanski D, Garcia A, Caraballo B, Thangeswaran P, Marin S, Bianco J, Lavi A, Poretsky L Tags: Cytokine Source Type: research
Effects of LncRNA Lnc-LIF-AS on cell proliferation, migration and invasion in a human cervical cancer cell line.
This study explored the effect of LncRNA Lnc-LIF-AS on cell proliferation, migration and invasion in the human cervical cancer (HCC) cell line SiHa. SiHa cells had the lowest expression of Lnc-LIF-AS in the 4 human cervical cancer cell lines (SiHa, ME-180, C-33A and HeLa) and were transfected and divided into the SiHa/con (transfected with pMIGRI) cell group, SiHa/Lnc-LIF-AS (transfected with pMIGRI-Lnc-LIF-AS) cell group, and SiHa/Lnc-LIF-AS-DN (transfected with pMIGRI-Lnc-LIF-AS-DN, in which the sequences overlapping with LIF mRNA was deleted) cell group. Overexpression of Lnc-LIF-AS could promote the proliferation, colo...
Source: Cytokine - May 11, 2019 Category: Molecular Biology Authors: Song W, Wang J, Liu H, Zhu C, Xu F, Qian L, Shen Z, Zhu J, Yin S, Qin J, Chen L, Wu D, Nashan B, Shan G, Xiao W, Zhou Y Tags: Cytokine Source Type: research
Mono- and poly-functional T cells in nontuberculous mycobacteria lung disease patients: Implications in analyzing risk of disease progression.
CONCLUSIONS: The patients with MAC/MAB-LD had attenuated poly-functional T cells. The triple-positive CD4+ cells could be useful in diagnosing disease from colonization. MAC-specific mono-IFN-γ+ CD4+ cells and triple positive-CD4+ might predict radiographic progression, which could be useful in making treatment decisions. PMID: 31085455 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - May 11, 2019 Category: Molecular Biology Authors: Shu CC, Wu LS, Wu MF, Lai HC, Wang PH, Cheng SL, Wang JY, Yu CJ Tags: Cytokine Source Type: research
Tunicamycin-induced endoplasmic reticulum stress up-regulates tumour-promoting cytokines in oral squamous cell carcinoma.
CONCLUSIONS: The gene and protein regulation patterns show that ERS plays a role in modifying the tumour microenvironment in OSCC by up-regulating tumour-promoting cytokines. PMID: 31071674 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - May 6, 2019 Category: Molecular Biology Authors: Yakin M, Seo B, Rich A Tags: Cytokine Source Type: research
Survival and immunomodulation of stem cells from human extracted deciduous teeth expanded in pooled human and foetal bovine sera.
In this study stem cells from human extracted deciduous teeth (SHED) were cultured in Knockout™ DMEM supplemented with either pooled human serum (pHS) or foetal bovine serum (FBS) to compare their suitability in maintaining immunomodulatory properties of cells during in vitro expansion. No significant difference in cell survival of SHED grown in pHS (pHS-SHED) or FBS (FBS-SHED) was observed when co-cultured with complement, monocytes or lymphocytes. However, significant changes in the expression of sixteen paracrine factors involved in immunomodulation were observed in the supernatants of FBS-SHED co-cultures with mo...
Source: Cytokine - May 6, 2019 Category: Molecular Biology Authors: Haque N, Khan IM, Abu Kasim NH Tags: Cytokine Source Type: research
Comparison of the chemokine profiles in the bronchoalveolar lavage fluid between IgG4-related respiratory disease and sarcoidosis: CC-chemokine ligand 1 might be involved in the pathogenesis of sarcoidosis.
We examined 12 chemokines using a MILLIPLEX® MAP Kit (Millipore, Darmstadt, Germany) in the same BALF samples of the same 44 patients (IgG4-RRD, n = 11; sarcoidosis, n = 33) in which we had previously evaluated the cytokines. RESULTS: The levels of CC-chemokine ligand (CCL)26 in the BALF of IgG4-RRD patients (median 24.5, range 3.1-401.1 pg/mL) were significantly higher than those in the BALF of sarcoidosis patients (median 3.1, range 3.1-155.6 pg/mL, p
Source: Cytokine - May 4, 2019 Category: Molecular Biology Authors: Yamamoto H, Yasuo M, Komatsu M, Ushiki A, Hamano H, Hori A, Nakajima T, Uehara T, Fujinaga Y, Matsui S, Hanaoka M Tags: Cytokine Source Type: research
Hyperactivation of the NLRP3 inflammasome protects mice against influenza A virus infection via IL-1 β mediated neutrophil recruitment.
Hyperactivation of the NLRP3 inflammasome protects mice against influenza A virus infection via IL-1β mediated neutrophil recruitment. Cytokine. 2019 May 02;120:115-124 Authors: Niu J, Wu S, Chen M, Xu K, Guo Q, Lu A, Zhao L, Sun B, Meng G Abstract Host innate immune system is critical for combating invading microbes including Influenza A virus (IAV). As an important arm of the innate immunity, the NLRP3 inflammasome has been found essential for protecting host against IAV challenge, while the mechanism remained elusive. Here we found that mice carrying a gain-of-function mutation in the Nlrp3 ge...
Source: Cytokine - May 2, 2019 Category: Molecular Biology Authors: Niu J, Wu S, Chen M, Xu K, Guo Q, Lu A, Zhao L, Sun B, Meng G Tags: Cytokine Source Type: research
Associations between intra-pancreatic fat deposition and circulating levels of cytokines.
Abstract While a plethora of studies have been conducted to investigate the associations between pro-inflammatory cytokines and obesity, the inter-relationship between pro-inflammatory cytokines and intra-pancreatic fat deposition (IPFD) has been poorly investigated. In the present study, circulating levels of C-C motif chemokine ligand 2 (CCL2), interleukin-6 (IL-6), leptin, and tumor necrosis factor-alpha (TNFα) were measured in 90 individuals after acute pancreatitis (AP) as well as 21 healthy non-obese individuals. Magnetic resonance imaging was used to quantify IPFD and visceral-to-subcutaneous fat volu...
Source: Cytokine - May 1, 2019 Category: Molecular Biology Authors: Singh RG, Nguyen NN, Cervantes A, Alarcon Ramos GC, Cho J, Petrov MS Tags: Cytokine Source Type: research
Hypocholesterolemia and dysregulated production of angiopoietin-like proteins in sickle cell anemia patients.
Abstract Angiopoietin-like proteins (ANGPTL) are responsible for inhibiting lipoprotein lipase activity, and ANGPTL3 and ANGPTL4 deficiencies have been shown to lower lipoprotein levels in animal models and in humans carrying loss-of-function mutations. Sickle cell anemia (SCA) is a hereditary hemolytic anemia characterized by vaso-occlusive crises and end-organ damage, which is curiously associated with hypocholesterolemia and a low incidence of atherosclerosis, whose underlying mechanisms are unclear. We hypothesized that ANGPTL3 and ANGPTL4 dysregulation is responsible for the hypolipidemic phenotype in SCA. We...
Source: Cytokine - May 1, 2019 Category: Molecular Biology Authors: Vendrame F, Olops L, Saad STO, Costa FF, Fertrin KY Tags: Cytokine Source Type: research
Interleukin-17 expression in the serum and exfoliated cervical cells of patients infected with high-risk oncogenic human papillomavirus.
Abstract Persistent infection by high-risk oncogenic human papillomavirus (HR-HPV) is the main cause of cervical cancer and its precursor lesions, and both the systemic and local immunological responses play an important role in eliminating or maintenance this infection. Th17 cells, as well as interleukin (IL)-17, are related to tumor growth and persistence of viral infection. Thus, this study aimed to quantify IL-17 in the serum and exfoliated cervical cells of HR-HPV-infected patients and healthy patients as well as identify CD4+IL17+ cells and IL-17 production in uterine cervix biopsies to better understand the...
Source: Cytokine - May 1, 2019 Category: Molecular Biology Authors: Mareti Bonin C, Zatorre Almeida-Lugo L, Rodrigues Dos Santos A, Tezelli Junqueira Padovani C, Silva Pina AF, Teixeira Ferreira AM, Dos Santos Fernandes CE, Possati Resende JC, Bovo AC, Tozetti IA Tags: Cytokine Source Type: research
Myostatin alters with exercise training in diabetic rats; possible interaction with glycosylated hemoglobin and inflammatory cytokines.
In conclusion, this study indicated that aerobic exercise training by a decrease of HbA1c and plasma IL-6, TNF-α and IL-1β could decrease MSTN levels in plasma and skeletal muscle in T1DM. Furthermore, the effective influence of exercise may be reflected by changes of MSTN in diabetic rats. PMID: 31054482 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - May 1, 2019 Category: Molecular Biology Authors: Kazemi F Tags: Cytokine Source Type: research
FLT3 ligand plasma levels have no impact on outcomes after allotransplant in acute leukemia.
CONCLUSION: At variance with induction results for AML (Peterlin et al., 2019) endogenous sFLc do not appear to be a prognostic marker at the time of or after allo-HSCT. Even though the results are negatives, this is, to the best of our knowledge, the only prospective series specifically addressing the question of sFLc impact after allo-HSCT in acute leukemias. PMID: 31035172 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - April 26, 2019 Category: Molecular Biology Authors: Peterlin P, Gaschet J, Guillaume T, Garnier A, Eveillard M, Le Bourgeois A, Cherel M, Debord C, Le Bris Y, Theisen O, Mahé B, Dubruille V, Godon C, Robillard N, Wuilleme S, Touzeau C, Gastinne T, Blin N, Lok A, Bonnet A, Le Gouill S, Moreau P, Béné MC, Tags: Cytokine Source Type: research
IL-23 and dendritic cells: What are the roles of their mutual attachment in immune response and immunotherapy?
Abstract Interleukin-23 (IL-23) is a cytokine that is composed of the subunits p19 and p40, while its receptor (IL-23R) consists of two subunits, that is, IL-23Rα and IL-12Rβ1. The interaction between IL-23 and IL-23R is necessary for exerting cardinal biological effects upon certain cell types, including promotion of memory T cell proliferation and Th17 cell-mediated IL-17 secretion. Accordingly, dendritic cells (DCs) are one of the main sources for IL-23 secretion. Interestingly, IL-23R is also present on the DC plasma membrane, suggesting that IL-23 potentially acts on DCs via an autocrine manner. In...
Source: Cytokine - April 24, 2019 Category: Molecular Biology Authors: Hua S, Yu X, Ma Y, Li Y Tags: Cytokine Source Type: research
Serum peptide profiling for potential biomarkers in early diagnosis of Escherichia coli bloodstream infection.
CONCLUSION: MALDI-TOF MS could be a new method to find the changes of serum peptides after infection, C3 and SAA1 could be new biomarkers in diagnosing BSI. PMID: 31026733 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - April 23, 2019 Category: Molecular Biology Authors: Ma Y, Chen C, Yang M, He S, Zhang K, Wang C Tags: Cytokine Source Type: research
Variations of salivary concentration of cytokines and chemokines in presence of oral squamous cell carcinoma. A case-crossover longitudinal prospective study.
Abstract Salivary biomarkers represent a promising diagnostic tool. Non-invasive and stress-free sampling results in objective parameters able to overcome the need of clinical expertise required for a correct interpretation of early oral squamous cell carcinoma (OSCC). The present study aims at evaluating potential variations in the salivary concentration of cytokines in presence of OSCC. Twenty patients who underwent surgical resection of OSCC were enrolled in order to assess the variation of the salivary concentration of cytokines and chemokines in a longitudinal prospective case-crossover setting. The salivary ...
Source: Cytokine - April 18, 2019 Category: Molecular Biology Authors: Val M, Sidoti Pinto GA, Manini L, Gandolfo S, Pentenero M Tags: Cytokine Source Type: research
Exercise-induced anti-inflammatory effects in overweight/obese women with polycystic ovary syndrome.
This study aimed to examine the potential effects of acute exercise on inflammatory responses in obese/overweight PCOS women and their controls. Participants underwent a single bout of moderate-intensity aerobic exercise (30 min at ∼65% of VO2peak). Blood and muscle samples were collected immediately before (PRE) and 60 min after the exercise session. Cytokines (i.e., IL-1β, IL-6, IL-4, IL-10, TNF-α) were measured both in plasma and in skeletal muscle, and proteins related to inflammatory signaling (IKKα/β and JNK) were assessed in skeletal muscle. At PRE, PCOS showed elevated muscle TNF-&alph...
Source: Cytokine - April 18, 2019 Category: Molecular Biology Authors: Dantas WS, Neves WD, Gil S, Barcellos CRG, Rocha MP, de Sá-Pinto AL, Roschel H, Gualano B Tags: Cytokine Source Type: research
Chemokines CCL2 and CCL7, but not CCL12, play a significant role in the development of pain-related behavior and opioid-induced analgesia.
Abstract The complex neuroimmunological interactions mediated by chemokines are suggested to be responsible for the development of neuropathic pain. The lack of knowledge regarding the detailed pathomechanism of neuropathy is one reason for the lack of optimally efficient therapies. Recently, several lines of evidence indicated that expression of CCR2 is increased in spinal cord neurons and microglial cells after peripheral nerve injury. It was previously shown that administration of CCR2 antagonists induces analgesic effects; however, the role of CCR2 ligands in neuropathic pain still needs to be explained. Thus,...
Source: Cytokine - April 16, 2019 Category: Molecular Biology Authors: Kwiatkowski K, Popiolek-Barczyk K, Piotrowska A, Rojewska E, Ciapała K, Makuch W, Mika J Tags: Cytokine Source Type: research
Interleukin-18 serum levels in sepsis: Correlation with disease severity and inflammatory markers.
CONCLUSION: The PCT, CRP and WBC levels revealed no significant differences between any analyzed subgroups in all time points during study. According to our results the IL-18 is a biomarker better differentiating sepsis and septic shock status than PCT, CRP and WBC but with no prognostic impact. PMID: 31003186 [PubMed - as supplied by publisher] (Source: Cytokine)
Source: Cytokine - April 16, 2019 Category: Molecular Biology Authors: Mierzchala-Pasierb M, Krzystek-Korpacka M, Lesnik P, Adamik B, Placzkowska S, Serek P, Gamian A, Lipinska-Gediga M Tags: Cytokine Source Type: research
Chicken viperin inhibits Newcastle disease virus infection in vitro: A possible interaction with the viral matrix protein.
Abstract Viperin is an interferon-inducible protein that helps in protecting mammals against various virus infections. Viperin is a highly conserved member of the interferon-stimulated genes (ISG) family in many species. Viperin has been shown to play a pivotal role in the innate immunity of chicken; however, its role has not been explored in its antiviral potential. Newcastle disease virus (NDV) is the causative agent of an infectious disease in poultry. In the present study, we have shown the anti-NDV effect of chicken viperin (cViperin). The impact of cViperin upon NDV infection was investigated in chicken embr...
Source: Cytokine - April 16, 2019 Category: Molecular Biology Authors: Shah M, Bharadwaj MSK, Gupta A, Kumar R, Kumar S Tags: Cytokine Source Type: research