Growth of Bacillus amyloliquefaciens as influence by Si nutrition
This study is the first to demonstrate the direct influence of active Si forms on bacteria growth. The research showed that monosilicic acid or Si-rich solid substances with high solubility on Si can be recommended to increase B. amyloliquefaciens growth in soil, water or reactors.PMID:34114085 | DOI:10.1007/s00203-021-02421-4 (Source: Archives of Microbiology)
Source: Archives of Microbiology - June 11, 2021 Category: Microbiology Authors: Vladimir Matichenkov Elena Bocharnikova Anastasia Romanova Phillipe Doullet Source Type: research

New insight into antimicrobial activities of Linaria ventricosa essential oil and its synergetic effect with conventional antibiotics
Arch Microbiol. 2021 Jun 11. doi: 10.1007/s00203-021-02391-7. Online ahead of print.ABSTRACTThe purpose of the present study was to determine for the first time the volatile constituents, the antioxidant and antimicrobial activities of the essential oil (EO) of the endemic Moroccan Linaria ventricosa, alone or in combination with four known antibiotics. The major constituents were 2-methoxy-4-vinylphenol (17.4%), α-terpinene (13.64%) and 3,5-dimethylphenyl isocyanate (12.21%). The EO had moderate antioxidant potency, as measured by DPPH free radical scavenging (1.233 ± 0.031 mg/mL), ferric reducing antioxidant...
Source: Archives of Microbiology - June 11, 2021 Category: Microbiology Authors: Ahmed Nafis Fatima Ezzahraa Saad Fatima El Khalloufi Ayoub Kasrati Abdelaziz Abbad Noureddine Mezrioui Brahim Oudra Vitor Vasconcelos Lahcen Hassani Source Type: research

Red fluorescent ultra-small gold nanoclusters functionalized with signal molecules to probe specificity in quorum sensing receptors in gram-negative bacteria
Arch Microbiol. 2021 Jun 9. doi: 10.1007/s00203-021-02338-y. Online ahead of print.ABSTRACTUltra-small (size
Source: Archives of Microbiology - June 10, 2021 Category: Microbiology Authors: Anurag M Chahande Disha Lathigara Asmita A Prabhune R Nandini Devi Source Type: research

Characterization of multidrug-resistant and virulent Klebsiella pneumoniae strains belonging to the high-risk clonal group 258 (CG258) isolated from inpatients in northeastern Brazil
This study aimed to characterize the antimicrobial resistance profile, virulence factors, and the clonal relationships among 13 K. pneumoniae strains belonging to CG258 from patients admitted to a tertiary hospital in Teresina, in the state of Piauí, northeastern Brazil. Ten strains were classified as MDR and three as extensively drug-resistant (XDR). Three different β-lactamase-encoding genes (blaKPC, blaOXA-1-like, and blaCTX-M-Gp1) and six virulence genes (fimH, ycfM, mrkD, entB, ybtS, and kfu) were detected. Moreover, two hypermucoviscous K. pneumoniae strains and one capsular K-type 2 were found. Multilocu...
Source: Archives of Microbiology - June 10, 2021 Category: Microbiology Authors: Rafael Nakamura-Silva Mariana Oliveira-Silva Jo ão Pedro Rueda Furlan Eliana Guedes Stehling Carlos Eduardo Saraiva Miranda Andr é Pitondo-Silva Source Type: research

Evaluation of the antioxidant, antibacterial, and antibiofilm activity of the sesquiterpene nerolidol
Arch Microbiol. 2021 Jun 10. doi: 10.1007/s00203-021-02377-5. Online ahead of print.ABSTRACTThe aim of this study was to evaluate the antioxidant, antibacterial, and antibiofilm activities of nerolidol. The antioxidant activity of nerolidol was determined using the total antioxidant activity method. Antibacterial activity was performed using the microdilution method to determine the minimum inhibitory concentration (MIC) against seven standard strains of the ATCC and four bacterial clinical isolates with a resistance profile, following the Clinical and Laboratory Standards Institute (CLSI). The antibiofilm activity of nero...
Source: Archives of Microbiology - June 10, 2021 Category: Microbiology Authors: Danielle Feij ó de Moura Tamiris Alves Rocha Dayane de Melo Barros Marllyn Marques da Silva Marcielle Dos Santos Santana Beatriz Mendes Neta Isabella Mac ário Ferro Cavalcanti Ren é Duarte Martins M árcia Vanusa da Silva Source Type: research

Optimization of (-)-cubebin biotransformation to (-)-hinokinin by the marine fungus Absidia coerulea 3A9
This study evaluates the ability of the Absidia coerulea 3A9 marine derived strain isolated from the ascidian Distaplia stilyfera to perform biotransformations by conducting assays with (-)-cubebin, as substrate. The experiment was optimized using the experimental design proposed by Plackett-Burman for seven factors and eight experiments, to establish the biotransformation conditions that would allow maximum production of biotransformed dibenzylbutyrolactone (-)-hinokinin. An analytical method based on Reverse-Phase-High Performance Liquid Chromatography (RP-HPLC) was developed to quantify the fungal biotransformation prod...
Source: Archives of Microbiology - June 10, 2021 Category: Microbiology Authors: Jonathan Messias de Souza Mario Ferreira Concei ção Santos Rita Cassia Nascimento Pedroso Leticia Pereira Pimenta K átia Aparecida Siqueira Marcos Antonio Soares Gustavo Muniz Dias Rosemeire Cristina Linhari Rodrigues Pietro Henrique Pereira Ramos Marc Source Type: research

Production, structural and biochemical characterization relevant to antitumor property of acidic exopolysaccharide produced from Bacillus sp. NRC5
This study targeted the production of exopolysaccharide from Bacillus sp. NRC5 grown in Egyptian seawater to use it as natural antitumor therapy. The biological activities of selected exopolysaccharide (BEPS) as an antioxidant, anti-inflammatory and anticancer have been studied. BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3). In addition, BEPS antitumor activity was tested against the Ehrlich Ascites Carcinoma (EAC) model. The BEPS presented potent antioxidant activities, consisted of ...
Source: Archives of Microbiology - June 10, 2021 Category: Microbiology Authors: Sahar S Mohamed Abeer Y Ibrahim Mohsen S Asker Manal G Mahmoud Samah A El-Newary Source Type: research

Screening and evaluation of laccase produced by different Trichoderma species along with their phylogenetic relationship
In this study, 10 Trichoderma species were screened for laccase enzyme production by submerged cultivation. The studied species were identified by internal transcribed spacer (ITS) gene sequences. Guaiacol (0.04%) as an enzyme substrate in plate medium was used for the selection of maximum laccase-enriched Trichoderma species by formation of visual color halo intensity. This activity was evaluated by liquid submersion (flask medium) also. The absorbance of laccase contained broth was measured by a spectrophotometer (450 nm). The highest laccase production was obtained by T. atroviride (2.62 U/mL). Trichoderma cremeum and T...
Source: Archives of Microbiology - June 10, 2021 Category: Microbiology Authors: Aisha Umar Source Type: research

Effect of pyrene and phenanthrene in shaping bacterial communities in seagrass meadows sediments
This study investigated the impact of two model PAHs (pyrene and phenanthrene) on bacterial community succession in the seagrass meadows sediment in a lab-scale microcosm. Halophila ovalis sediment slurry microcosms were established, one group was placed as a control, and the other two were treated with pyrene and phenanthrene. Bacterial community succession in response to respective PAHs was investigated by 16S rRNA amplicon sequencing. The results demonstrated that bacterial diversity decrease in each microcosm during the incubation process; however, the composition of bacterial communities in each microcosm was signific...
Source: Archives of Microbiology - June 8, 2021 Category: Microbiology Authors: Manzoor Ahmad Juan Ling Yanying Zhang Wasim Sajjad Qingsong Yang Weiguo Zhou Junde Dong Source Type: research

Effect of co-application of Trichoderma spp. with organic composts on plant growth enhancement, soil enzymes and fungal community in soil
In conclusion, Trichoderma sp. RW309 could be considered for use as a bioorganic fertilizer in combination with organic compost to minimize the use of mineral fertilizers.PMID:34100101 | DOI:10.1007/s00203-021-02413-4 (Source: Archives of Microbiology)
Source: Archives of Microbiology - June 8, 2021 Category: Microbiology Authors: Waleed Asghar Ryota Kataoka Source Type: research

Effect of pyrene and phenanthrene in shaping bacterial communities in seagrass meadows sediments
This study investigated the impact of two model PAHs (pyrene and phenanthrene) on bacterial community succession in the seagrass meadows sediment in a lab-scale microcosm. Halophila ovalis sediment slurry microcosms were established, one group was placed as a control, and the other two were treated with pyrene and phenanthrene. Bacterial community succession in response to respective PAHs was investigated by 16S rRNA amplicon sequencing. The results demonstrated that bacterial diversity decrease in each microcosm during the incubation process; however, the composition of bacterial communities in each microcosm was signific...
Source: Archives of Microbiology - June 8, 2021 Category: Microbiology Authors: Manzoor Ahmad Juan Ling Yanying Zhang Wasim Sajjad Qingsong Yang Weiguo Zhou Junde Dong Source Type: research

Effect of co-application of Trichoderma spp. with organic composts on plant growth enhancement, soil enzymes and fungal community in soil
In conclusion, Trichoderma sp. RW309 could be considered for use as a bioorganic fertilizer in combination with organic compost to minimize the use of mineral fertilizers.PMID:34100101 | DOI:10.1007/s00203-021-02413-4 (Source: Archives of Microbiology)
Source: Archives of Microbiology - June 8, 2021 Category: Microbiology Authors: Waleed Asghar Ryota Kataoka Source Type: research

Effect of pyrene and phenanthrene in shaping bacterial communities in seagrass meadows sediments
This study investigated the impact of two model PAHs (pyrene and phenanthrene) on bacterial community succession in the seagrass meadows sediment in a lab-scale microcosm. Halophila ovalis sediment slurry microcosms were established, one group was placed as a control, and the other two were treated with pyrene and phenanthrene. Bacterial community succession in response to respective PAHs was investigated by 16S rRNA amplicon sequencing. The results demonstrated that bacterial diversity decrease in each microcosm during the incubation process; however, the composition of bacterial communities in each microcosm was signific...
Source: Archives of Microbiology - June 8, 2021 Category: Microbiology Authors: Manzoor Ahmad Juan Ling Yanying Zhang Wasim Sajjad Qingsong Yang Weiguo Zhou Junde Dong Source Type: research

Effect of co-application of Trichoderma spp. with organic composts on plant growth enhancement, soil enzymes and fungal community in soil
In conclusion, Trichoderma sp. RW309 could be considered for use as a bioorganic fertilizer in combination with organic compost to minimize the use of mineral fertilizers.PMID:34100101 | DOI:10.1007/s00203-021-02413-4 (Source: Archives of Microbiology)
Source: Archives of Microbiology - June 8, 2021 Category: Microbiology Authors: Waleed Asghar Ryota Kataoka Source Type: research

Molecular docking study of bio-inhibitors extracted from marine macro-alga Ulva fasciata against hemolysin protein of luminescence disease-causing Vibrio harveyi
In this study, marine macro-alga Ulva fasciata was aimed at developing inhibitors against luminescence disease-causing pathogenic bacteria Vibrio harveyi. U. fasciata was collected from Thoothukudi, Tamil Nadu, India. Extract of U. fasciata was tested against growth and virulence factors of V. harveyi during Penaeus monodon larviculture. Further U. fasciata extract was subjected to GC-MS analysis to identify the biomolecules. The homology modeling of virulent protein, hemolysin of V. harveyi was designed in this study. Hence, it was aimed for molecular docking against the biomolecules identified from U. fasciata extract. D...
Source: Archives of Microbiology - June 7, 2021 Category: Microbiology Authors: Krishnamoorthy Sivakumar Sudalayandi Kannappan Balakrishnan Vijayakumar Karingalakkandy Poochirian Jithendran Sivamani Balasubramaniam Akshaya Panigrahi Source Type: research

Survival of the bla < sub > NDM < /sub > -harbouring Escherichia coli in tropical seawater and conjugative transfer of resistance markers
This study was conducted to understand the survivability of a multidrug-resistant, the New Delhi Metallo-β-lactamase-producing E. coli (AS-EC121) in tropical seawater at room temperature (28-32 °C) compared to E. coli K12 strain. The experimental and control strains were inoculated at 6 log CFU/ml level into seawater. After an initial sharp decline in counts, AS-EC121 and K12 strains showed a gradual loss of viability after week-1 of inoculation. AS-EC121 was undetectable after day-56, while K12 colonies disappeared a week later, from day-63. The conjugation experiment revealed that pNDM was transferable to a reci...
Source: Archives of Microbiology - June 7, 2021 Category: Microbiology Authors: Susmita Mukherjee Manjusha Lekshmi Parvathi Ammini Binaya Bhusan Nayak Sanath H Kumar Source Type: research

Molecular docking study of bio-inhibitors extracted from marine macro-alga Ulva fasciata against hemolysin protein of luminescence disease-causing Vibrio harveyi
In this study, marine macro-alga Ulva fasciata was aimed at developing inhibitors against luminescence disease-causing pathogenic bacteria Vibrio harveyi. U. fasciata was collected from Thoothukudi, Tamil Nadu, India. Extract of U. fasciata was tested against growth and virulence factors of V. harveyi during Penaeus monodon larviculture. Further U. fasciata extract was subjected to GC-MS analysis to identify the biomolecules. The homology modeling of virulent protein, hemolysin of V. harveyi was designed in this study. Hence, it was aimed for molecular docking against the biomolecules identified from U. fasciata extract. D...
Source: Archives of Microbiology - June 7, 2021 Category: Microbiology Authors: Krishnamoorthy Sivakumar Sudalayandi Kannappan Balakrishnan Vijayakumar Karingalakkandy Poochirian Jithendran Sivamani Balasubramaniam Akshaya Panigrahi Source Type: research

Survival of the bla < sub > NDM < /sub > -harbouring Escherichia coli in tropical seawater and conjugative transfer of resistance markers
This study was conducted to understand the survivability of a multidrug-resistant, the New Delhi Metallo-β-lactamase-producing E. coli (AS-EC121) in tropical seawater at room temperature (28-32 °C) compared to E. coli K12 strain. The experimental and control strains were inoculated at 6 log CFU/ml level into seawater. After an initial sharp decline in counts, AS-EC121 and K12 strains showed a gradual loss of viability after week-1 of inoculation. AS-EC121 was undetectable after day-56, while K12 colonies disappeared a week later, from day-63. The conjugation experiment revealed that pNDM was transferable to a reci...
Source: Archives of Microbiology - June 7, 2021 Category: Microbiology Authors: Susmita Mukherjee Manjusha Lekshmi Parvathi Ammini Binaya Bhusan Nayak Sanath H Kumar Source Type: research

A new Streptomyces scabies-infecting bacteriophage from Egypt with promising biocontrol traits
In this study, a novel bacteriophage with high lytic efficacy against S. scabies was isolated from a potato field at El-Minya, Egypt, and was designated SscP1EGY. The phage has an icosahedral head of 55 nm and a short tail of 7.5 nm, typical of a podovirus. Its infection cycle was 90 min, including 50 min of latent time and 40 min of rise period with a burst size of approximately 200 PFU per infected cell. The genome of SscP1EGY consists 51,751 nucleotides with 76 predicted genes. SscP1EGY infected and completely lysed seven tested S. scabies strains but showed no lytic activity against three beneficial Streptomyces specie...
Source: Archives of Microbiology - June 6, 2021 Category: Microbiology Authors: Abdelrazek S Abdelrhim Abdelmonim Ali Ahmad Maha O A Omar Adel M M Hammad Qi Huang Source Type: research

Mechanism of nitrite transporter NirC in motility, biofilm formation, and adhesion of avian pathogenic Escherichia coli
Arch Microbiol. 2021 Jun 5. doi: 10.1007/s00203-021-02412-5. Online ahead of print.ABSTRACTThe Escherichia coli (E. coli) nirC gene encodes a nitrite transporter, which involved in transporting toxic nitrite (NO2-) from the environment into the bacteria. Although the deletion of nirC gene could cause changes in motility, adhesion in the previous study, and the virulence involved in the specified mechanism for pathogenic E. coli remains to be known. In the present work, we aimed to evaluate the role of NirC in a serotype O2:K1:H7 avian pathogenic Escherichia coli (APEC) strain. For this purpose, we generated a NirC-deficien...
Source: Archives of Microbiology - June 6, 2021 Category: Microbiology Authors: Jiaqi Liu Dong Zhang Siqi Lian Xuanqiang Gu Qianxi Hou Pengpeng Xia Guoqiang Zhu Source Type: research

Flavobacterium agrisoli sp. nov., a novel bacterium isolated from soil
Arch Microbiol. 2021 Jun 3. doi: 10.1007/s00203-021-02376-6. Online ahead of print.ABSTRACTA Gram-stain negative, rod shaped, motile by gliding, yellow-pigmented, aerobic bacterium, designated SE-1-eT, was isolated from a soil sample collected on Dokdo Island, South Korea. The isolate was characterized taxonomically using a polyphasic approach based on the phenotypic and genomic analyses. The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SE-1-eT belonged to the genus Flavobacterium in the family Flavobacteriaceae and had the highest sequence similarity with Flavobacterium cheongpyeongense IMCC3...
Source: Archives of Microbiology - June 4, 2021 Category: Microbiology Authors: Weilan Li Leonid N Ten Myung Kyum Kim Seung-Yeol Lee Hee-Young Jung Source Type: research

Recent advances in gold nanoparticle-based lateral flow immunoassay for the detection of bacterial infection
Arch Microbiol. 2021 Jun 4. doi: 10.1007/s00203-021-02357-9. Online ahead of print.ABSTRACTDiagnosis of bacterial infections (BI) is becoming an increasingly difficult task in clinical practice due to their high prevalence and frequency, as well as the growth of antibiotic resistance worldwide. World Health Organization (WHO) reported antibiotic resistance is a major public health problem. BI becomes difficult or impossible to treat when the bacteria acquire immunity against antibiotics. Thus, there is a need for a quick and accurate technique to detect infection. Lateral flow immunoassay (LFIA) is an ideal technique for p...
Source: Archives of Microbiology - June 4, 2021 Category: Microbiology Authors: Yachana Gupta Aditya Sharma Ghrera Source Type: research

Flavobacterium agrisoli sp. nov., a novel bacterium isolated from soil
Arch Microbiol. 2021 Jun 3. doi: 10.1007/s00203-021-02376-6. Online ahead of print.ABSTRACTA Gram-stain negative, rod shaped, motile by gliding, yellow-pigmented, aerobic bacterium, designated SE-1-eT, was isolated from a soil sample collected on Dokdo Island, South Korea. The isolate was characterized taxonomically using a polyphasic approach based on the phenotypic and genomic analyses. The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SE-1-eT belonged to the genus Flavobacterium in the family Flavobacteriaceae and had the highest sequence similarity with Flavobacterium cheongpyeongense IMCC3...
Source: Archives of Microbiology - June 4, 2021 Category: Microbiology Authors: Weilan Li Leonid N Ten Myung Kyum Kim Seung-Yeol Lee Hee-Young Jung Source Type: research

Recent advances in gold nanoparticle-based lateral flow immunoassay for the detection of bacterial infection
Arch Microbiol. 2021 Jun 4. doi: 10.1007/s00203-021-02357-9. Online ahead of print.ABSTRACTDiagnosis of bacterial infections (BI) is becoming an increasingly difficult task in clinical practice due to their high prevalence and frequency, as well as the growth of antibiotic resistance worldwide. World Health Organization (WHO) reported antibiotic resistance is a major public health problem. BI becomes difficult or impossible to treat when the bacteria acquire immunity against antibiotics. Thus, there is a need for a quick and accurate technique to detect infection. Lateral flow immunoassay (LFIA) is an ideal technique for p...
Source: Archives of Microbiology - June 4, 2021 Category: Microbiology Authors: Yachana Gupta Aditya Sharma Ghrera Source Type: research

Flavobacterium agrisoli sp. nov., a novel bacterium isolated from soil
Arch Microbiol. 2021 Jun 3. doi: 10.1007/s00203-021-02376-6. Online ahead of print.ABSTRACTA Gram-stain negative, rod shaped, motile by gliding, yellow-pigmented, aerobic bacterium, designated SE-1-eT, was isolated from a soil sample collected on Dokdo Island, South Korea. The isolate was characterized taxonomically using a polyphasic approach based on the phenotypic and genomic analyses. The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SE-1-eT belonged to the genus Flavobacterium in the family Flavobacteriaceae and had the highest sequence similarity with Flavobacterium cheongpyeongense IMCC3...
Source: Archives of Microbiology - June 4, 2021 Category: Microbiology Authors: Weilan Li Leonid N Ten Myung Kyum Kim Seung-Yeol Lee Hee-Young Jung Source Type: research

Recent advances in gold nanoparticle-based lateral flow immunoassay for the detection of bacterial infection
Arch Microbiol. 2021 Jun 4. doi: 10.1007/s00203-021-02357-9. Online ahead of print.ABSTRACTDiagnosis of bacterial infections (BI) is becoming an increasingly difficult task in clinical practice due to their high prevalence and frequency, as well as the growth of antibiotic resistance worldwide. World Health Organization (WHO) reported antibiotic resistance is a major public health problem. BI becomes difficult or impossible to treat when the bacteria acquire immunity against antibiotics. Thus, there is a need for a quick and accurate technique to detect infection. Lateral flow immunoassay (LFIA) is an ideal technique for p...
Source: Archives of Microbiology - June 4, 2021 Category: Microbiology Authors: Yachana Gupta Aditya Sharma Ghrera Source Type: research

Broad diversity of bacteria degrading 17 ß-estradiol-3-sulfate isolated from river sediment and biofilm at a wastewater treatment plant discharge
Arch Microbiol. 2021 Jun 3. doi: 10.1007/s00203-021-02409-0. Online ahead of print.ABSTRACTConjugated estrogens, such as 17β-estradiol-3-sulfate (E2-3S), can be released into aquatic environments through wastewater treatment plants (WWTP). There, they are microbiologically degraded into free estrogens, which can have harmful effects on aquatic wildlife. Here, the degradation of E2-3S in environmental samples taken upstream, downstream and at the effluent of a WWTP was assessed. Sediment and biofilm samples were enriched for E2-3S-degrading microorganisms, yielding a broad diversity of bacterial isolates, including kno...
Source: Archives of Microbiology - June 3, 2021 Category: Microbiology Authors: Tamara Mainetti Marilena Palmisano Fabio Rezzonico Bla ž Stres Susanne Kern Theo H M Smits Source Type: research

Lysinibacillus agricola sp. nov., isolated from soil
Arch Microbiol. 2021 Jun 1. doi: 10.1007/s00203-021-02394-4. Online ahead of print.ABSTRACTA gram-staining-positive, rod-shaped bacterium, designed strain FJAT-51161T was isolated from farmland soil collected from Fujian Province, China. Growth was observed at 25-40 °C (optimum 30 °C), pH 7.0-9.0 (optimum 7.0), and NaCl tolerance in the range of 0-7% (w/v), respectively. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain FJAT-51161T belonged to the genus Lysinibacillus, and had the closest relationship with Lysinibacillus xylanilyticus XDB9T (99.0% 16S rRNA sequence similarity). Th...
Source: Archives of Microbiology - June 2, 2021 Category: Microbiology Authors: Jia-Rui Lu Guo-Hong Liu Source Type: research

Diversity and biopotential of Bacillus velezensis strains A6 and P42 against rice blast and bacterial blight of pomegranate
Arch Microbiol. 2021 Jun 2. doi: 10.1007/s00203-021-02400-9. Online ahead of print.ABSTRACTBacillus velezensis is widely known for its inherent biosynthetic potential to produce a wide range of bio-macromolecules and secondary metabolites, including polyketides (PKs) and siderophores, as well as ribosomally and non-ribosomally synthesized peptides. In the present study, we aimed to investigate the bio-macromolecules, such as proteins and peptides of Bacillus velezensis strains, namely A6 and P42 by whole-cell sequencing and highlighted the potential application in controlling phytopathogens. The bioactive compounds, specif...
Source: Archives of Microbiology - June 2, 2021 Category: Microbiology Authors: Siddulakshmi Prasanna M K Prasannakumar H B Mahesh Gopal Venkatesh Babu P Kirnaymayee M E Puneeth Karthik S Narayan D Pramesh Source Type: research

Bioactive potential evaluation and purification of compounds from an endophytic fungus Diaporthe longicolla, a resident of Saraca asoca (Roxb.) Willd
Arch Microbiol. 2021 Jun 2. doi: 10.1007/s00203-021-02390-8. Online ahead of print.ABSTRACTAn endophytic fungus (L3), isolated from the leaf tissues of Saraca asoca was identified as D. longicolla by microscopic and molecular methods. The crude extracts of D. longicolla revealed to harbor seven compounds in GC-MS analysis which was subjected to a thin layer chromatography (TLC) for purification and separation of bioactive ingredients. The partially purified fraction from TLC displayed the presence of 2-tridecene (Z) (RT-14.50), 5-tridecene (E) (RT-16.65) and 2,4-di-tert-butylphenol (RT-13.92) in GC-MS. High-performance liq...
Source: Archives of Microbiology - June 2, 2021 Category: Microbiology Authors: Jay Hind Nishad Arti Singh Veer Singh Gautam Puja Kumari Jitendra Kumar Monika Yadav Ravindra Nath Kharwar Source Type: research

Genomic analysis of high copy-number sequences for the targeted detection of Listeria species using a flow-through surveillance system
Arch Microbiol. 2021 Jun 2. doi: 10.1007/s00203-021-02388-2. Online ahead of print.ABSTRACTThe bacterial foodborne pathogen Listeria monocytogenes has been implicated in fresh produce outbreaks with a significant economic impact. Given that L. monocytogenes is widespread in the environment, food production facilities constantly monitor for the presence of Listeria species. To develop a surveillance platform for food processing facilities, this study conducted a comparative genomic analysis for the identification of conserved high copy sequences in the ribosomal RNA of Listeria species. Simulated folding was performed to as...
Source: Archives of Microbiology - June 2, 2021 Category: Microbiology Authors: Beatriz Qui ñones Jaszemyn C Yambao Veronica S De Guzman Bertram G Lee David L Medin Source Type: research

Lysinibacillus agricola sp. nov., isolated from soil
Arch Microbiol. 2021 Jun 1. doi: 10.1007/s00203-021-02394-4. Online ahead of print.ABSTRACTA gram-staining-positive, rod-shaped bacterium, designed strain FJAT-51161T was isolated from farmland soil collected from Fujian Province, China. Growth was observed at 25-40 °C (optimum 30 °C), pH 7.0-9.0 (optimum 7.0), and NaCl tolerance in the range of 0-7% (w/v), respectively. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain FJAT-51161T belonged to the genus Lysinibacillus, and had the closest relationship with Lysinibacillus xylanilyticus XDB9T (99.0% 16S rRNA sequence similarity). Th...
Source: Archives of Microbiology - June 2, 2021 Category: Microbiology Authors: Jia-Rui Lu Guo-Hong Liu Source Type: research

Diversity and biopotential of Bacillus velezensis strains A6 and P42 against rice blast and bacterial blight of pomegranate
Arch Microbiol. 2021 Jun 2. doi: 10.1007/s00203-021-02400-9. Online ahead of print.ABSTRACTBacillus velezensis is widely known for its inherent biosynthetic potential to produce a wide range of bio-macromolecules and secondary metabolites, including polyketides (PKs) and siderophores, as well as ribosomally and non-ribosomally synthesized peptides. In the present study, we aimed to investigate the bio-macromolecules, such as proteins and peptides of Bacillus velezensis strains, namely A6 and P42 by whole-cell sequencing and highlighted the potential application in controlling phytopathogens. The bioactive compounds, specif...
Source: Archives of Microbiology - June 2, 2021 Category: Microbiology Authors: Siddulakshmi Prasanna M K Prasannakumar H B Mahesh Gopal Venkatesh Babu P Kirnaymayee M E Puneeth Karthik S Narayan D Pramesh Source Type: research

Bioactive potential evaluation and purification of compounds from an endophytic fungus Diaporthe longicolla, a resident of Saraca asoca (Roxb.) Willd
Arch Microbiol. 2021 Jun 2. doi: 10.1007/s00203-021-02390-8. Online ahead of print.ABSTRACTAn endophytic fungus (L3), isolated from the leaf tissues of Saraca asoca was identified as D. longicolla by microscopic and molecular methods. The crude extracts of D. longicolla revealed to harbor seven compounds in GC-MS analysis which was subjected to a thin layer chromatography (TLC) for purification and separation of bioactive ingredients. The partially purified fraction from TLC displayed the presence of 2-tridecene (Z) (RT-14.50), 5-tridecene (E) (RT-16.65) and 2,4-di-tert-butylphenol (RT-13.92) in GC-MS. High-performance liq...
Source: Archives of Microbiology - June 2, 2021 Category: Microbiology Authors: Jay Hind Nishad Arti Singh Veer Singh Gautam Puja Kumari Jitendra Kumar Monika Yadav Ravindra Nath Kharwar Source Type: research

Genomic analysis of high copy-number sequences for the targeted detection of Listeria species using a flow-through surveillance system
Arch Microbiol. 2021 Jun 2. doi: 10.1007/s00203-021-02388-2. Online ahead of print.ABSTRACTThe bacterial foodborne pathogen Listeria monocytogenes has been implicated in fresh produce outbreaks with a significant economic impact. Given that L. monocytogenes is widespread in the environment, food production facilities constantly monitor for the presence of Listeria species. To develop a surveillance platform for food processing facilities, this study conducted a comparative genomic analysis for the identification of conserved high copy sequences in the ribosomal RNA of Listeria species. Simulated folding was performed to as...
Source: Archives of Microbiology - June 2, 2021 Category: Microbiology Authors: Beatriz Qui ñones Jaszemyn C Yambao Veronica S De Guzman Bertram G Lee David L Medin Source Type: research

Effect of a sugarcane cystatin on the profile and viability of microcosm biofilm and on dentin demineralization
Arch Microbiol. 2021 May 31. doi: 10.1007/s00203-021-02403-6. Online ahead of print.ABSTRACTTo analyze the effect of a sugarcane cystatin (CaneCPI-5) on the microbial profile and viability, as well as on the prevention of dentin demineralization using a microcosm biofilm model. Ninety bovine dentine specimens were divided into five experimental groups according with the solution they were treated for 60 s: (1) PBS (negative control), (2) 0.12% chlorhexidine (positive control), (3) Fluoride (500 ppm F, as NaF), (4) 0.025 mg/ml CaneCPI-5, and (5) 0.05 mg/ml CaneCPI-5. Specimens were incubated with inoculum (McBain's saliva p...
Source: Archives of Microbiology - June 1, 2021 Category: Microbiology Authors: Jo ão Victor Frazão Câmara Tamara Teodoro Araujo Daniela Alejandra Cusicanqui Mendez Natara Dias Gomes da Silva Felipe F ávaro de Medeiros Lethycia Almeida Santos Thamyris de Souza Carvalho Fabiana Navas Reis Tatiana Martini Samanta Mascarenhas Moraes Source Type: research

Benzylmalonyl-CoA dehydrogenase, an enzyme involved in bacterial auxin degradation
Arch Microbiol. 2021 May 31. doi: 10.1007/s00203-021-02406-3. Online ahead of print.ABSTRACTA novel acyl-CoA dehydrogenase involved in degradation of the auxin indoleacetate by Aromatoleum aromaticum was identified as a decarboxylating benzylmalonyl-CoA dehydrogenase (IaaF). It is encoded within the iaa operon coding for enzymes of indoleacetate catabolism. Using enzymatically produced benzylmalonyl-CoA, the reaction was characterized as simultaneous oxidation and decarboxylation of benzylmalonyl-CoA to cinnamoyl-CoA and CO2. Oxygen served as electron acceptor and was reduced to H2O2, whereas electron transfer flavoprotein...
Source: Archives of Microbiology - June 1, 2021 Category: Microbiology Authors: Karola Sch ühle Martin Saft Bastian V ögeli Tobias J Erb Johann Heider Source Type: research

In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice
The objective of this work was to determine the antibacterial effect of Lactobacillus plantarum strains of pork origin against Campylobacter coli strains, and to conduct experimental colonization pilot models in mice for both microorganisms. Inhibition assays allowed evaluation and selection of L. plantarum LP5 as the strain with the highest antagonistic activity against C. coli and with the best potential to be used in in vivo study. Adult 6-week-old female Balb/cCmedc mice were lodged in two groups. The treated group was administered with 9.4 log10CFU/2 times/wk of L. plantarum LP5. L. plantarum LP5 was recovered from th...
Source: Archives of Microbiology - June 1, 2021 Category: Microbiology Authors: M J Ruiz M V Zbrun M L Signorini J A Zimmermann L P Soto M R Rosmini L S Frizzo Source Type: research

Effect of a sugarcane cystatin on the profile and viability of microcosm biofilm and on dentin demineralization
Arch Microbiol. 2021 May 31. doi: 10.1007/s00203-021-02403-6. Online ahead of print.ABSTRACTTo analyze the effect of a sugarcane cystatin (CaneCPI-5) on the microbial profile and viability, as well as on the prevention of dentin demineralization using a microcosm biofilm model. Ninety bovine dentine specimens were divided into five experimental groups according with the solution they were treated for 60 s: (1) PBS (negative control), (2) 0.12% chlorhexidine (positive control), (3) Fluoride (500 ppm F, as NaF), (4) 0.025 mg/ml CaneCPI-5, and (5) 0.05 mg/ml CaneCPI-5. Specimens were incubated with inoculum (McBain's saliva p...
Source: Archives of Microbiology - June 1, 2021 Category: Microbiology Authors: Jo ão Victor Frazão Câmara Tamara Teodoro Araujo Daniela Alejandra Cusicanqui Mendez Natara Dias Gomes da Silva Felipe F ávaro de Medeiros Lethycia Almeida Santos Thamyris de Souza Carvalho Fabiana Navas Reis Tatiana Martini Samanta Mascarenhas Moraes Source Type: research

Benzylmalonyl-CoA dehydrogenase, an enzyme involved in bacterial auxin degradation
Arch Microbiol. 2021 May 31. doi: 10.1007/s00203-021-02406-3. Online ahead of print.ABSTRACTA novel acyl-CoA dehydrogenase involved in degradation of the auxin indoleacetate by Aromatoleum aromaticum was identified as a decarboxylating benzylmalonyl-CoA dehydrogenase (IaaF). It is encoded within the iaa operon coding for enzymes of indoleacetate catabolism. Using enzymatically produced benzylmalonyl-CoA, the reaction was characterized as simultaneous oxidation and decarboxylation of benzylmalonyl-CoA to cinnamoyl-CoA and CO2. Oxygen served as electron acceptor and was reduced to H2O2, whereas electron transfer flavoprotein...
Source: Archives of Microbiology - June 1, 2021 Category: Microbiology Authors: Karola Sch ühle Martin Saft Bastian V ögeli Tobias J Erb Johann Heider Source Type: research

LuxS quorum sensing system mediating Lactobacillus plantarum probiotic characteristics
Arch Microbiol. 2021 May 31. doi: 10.1007/s00203-021-02404-5. Online ahead of print.ABSTRACTLactobacillus plantarum is one of common probiotics in fermented foods. Quorum sensing (QS) is a common communication way within bacteria. It is not clear whether the probiotic properties of L. plantarum mediated by QS. Here, Lb. plantarum YM-4-3 was examined for resistance of pH, bile, antimicrobial and luxS gene expression pattern. The study found that: (1) the supernatant of YM-4-3 had bacteriostatic effect to Escherichia coli O157:H7, Listeria monocytogenes and Staphylococcus aureus; (2) Lb. plantarum YM-4-3 shown tolerance prop...
Source: Archives of Microbiology - May 31, 2021 Category: Microbiology Authors: Liming Jiang Yiyong Luo Xuebin Cao Wen Liu Gang Song Zhizhen Zhang Source Type: research

High expression of ring-hydroxylating dioxygenase genes ensure efficient degradation of p-toluate, phthalate, and terephthalate by Comamonas testosteroni strain 3a2
This study showed that the isolate 3a2 can produce terephthalate and phthalate during the para-toluic acid biodegradation, which were also degraded after 24 h.PMID:34057546 | DOI:10.1007/s00203-021-02395-3 (Source: Archives of Microbiology)
Source: Archives of Microbiology - May 31, 2021 Category: Microbiology Authors: Didem Aksu Mamadou Malick Diallo Umut Şahar Tayyibe Alpay Uyaniker Guven Ozdemir Source Type: research

Bacillus fonticola sp. nov., isolated from deep sea cold seep sediment
In this study, we report a novel Gram-positive bacterium, designated as strain CS13T, isolated from deep-sea sediment collected in the cold seep area of the South China Sea. Growth of strain CS13T occurred at 16-37 °C (optimum 25-28 °C), pH 7.0-9.0 (optimum, 7.0), and 0-8% (w/v) NaCl (optimum, 2-3%). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CS13T belonged to the genus Bacillus. The closest phylogenetic neighbors of strain CS13T are Bacillus carboniphilus JCM 9731T (96.0%), Bacillus pakistanensis NCCP-168T (95.7%) and Bacillus acidicola 105-2T (95.6%). The genomic DNA G + C content...
Source: Archives of Microbiology - May 31, 2021 Category: Microbiology Authors: Yuan-Yuan Sun Hai-Zhen Zhou Qing-Lei Sun Source Type: research

Identification and characterization of a novel carboxylesterase EstQ7 from a soil metagenomic library
Arch Microbiol. 2021 May 31. doi: 10.1007/s00203-021-02398-0. Online ahead of print.ABSTRACTA novel lipolytic gene, estq7, was identified from a fosmid metagenomic library. The recombinant enzyme EstQ7 consists of 370 amino acids with an anticipated molecular mass of 42 kDa. Multiple sequence alignments showed that EstQ7 contained a pentapeptide motif GHSMG, and a putative catalytic triad Ser174-Asp306-His344. Interestingly, EstQ7 was found to have very little similarity to the characterized lipolytic enzymes. Phylogenetic analysis revealed that EstQ7 may be a member of a novel family of lipolytic enzymes. Biochemical char...
Source: Archives of Microbiology - May 31, 2021 Category: Microbiology Authors: Zhenzhen Yan Liping Ding Dandan Zou Luyao Wang Yuzhi Tan Shuting Guo Yingchen Zhang Zhihong Xin Source Type: research

Chemical composition and potentiating action of Norfloxacin mediated by the essential oil of Piper caldense C.D.C. against Staphylococcus aureus strains overexpressing efflux pump genes
This study had as objectives characterize the chemical composition of the essential oil from Piper caldense (EOPC), evaluate its potential antimicrobial activity, and investigate the synergistic effect with Norfloxacin against multidrug-resistant S. aureus overproducing efflux pumps, as well as, verify the EOPC ability to inhibit the Candida albicans filamentation. EOPC was extracted by hydrodistillation, and the chemical constituents were identified by gas chromatography, allowing the identification of 24 compounds (91.9%) classified as hydrocarbon sesquiterpenes (49.6%) and oxygenated sesquiterpenes (39.5%). Antimicrobia...
Source: Archives of Microbiology - May 30, 2021 Category: Microbiology Authors: Antonio Linkoln Alves Borges Leal Camila Fonseca Bezerra Camila Confortin Luiz Everson da Silva Emanuelle Machado Marinho M árcia Machado Marinho Mayron Alves Vasconcelos Terezinha Gon çalves da Silva Emmanuel Silva Marinho Alexandre Magno Rodrigues Tei Source Type: research

Enhanced production of prodigiosin by Serratia marcescens UCP 1549 using agrosubstrates in solid-state fermentation
Arch Microbiol. 2021 May 30. doi: 10.1007/s00203-021-02399-z. Online ahead of print.ABSTRACTThis work aimed to investigate the production of prodigiosin by S. marcescens UCP 1549 in solid-state fermentation (SSF), as a sustainable alternative for reducing the production costs and environmental impact. Thus, different agro-industrial substrates were used in the formulation of the prodigiosin production medium, obtaining the maximum yield of pigment (119.8 g/kg dry substrate) in medium consisting of 5 g wheat bran, 5% waste soybean oil and saline solution. The pigment was confirmed as prodigiosin by the maximum absorbance pe...
Source: Archives of Microbiology - May 30, 2021 Category: Microbiology Authors: Renata Andreia Dos Santos Dayana Montero Rodr íguez Lucas Albuquerque Rosendo da Silva S érgio Mendonça de Almeida Galba Maria de Campos-Takaki Marcos Ant ônio Barbosa de Lima Source Type: research

Metallococcus carri gen. nov., sp. nov., a novel member of the family Dermacoccaceae isolated from an automotive air conditioning system
Arch Microbiol. 2021 May 30. doi: 10.1007/s00203-021-02386-4. Online ahead of print.ABSTRACTStrain designated DB0510T was isolated from an automobile evaporator core collected in Korea. Cells are gram-stain-positive, aerobic, and coccoid. The strain grew at 15-45 ℃, pH 5.0-8.5 and 0-8.0% (w/v) NaCl. Growth occurs on R2A, trypticase soy agar, Luria-Bertani agar, and nutrient agar. Phylogenetic analysis showed that the strain belongs to the family Dermacoccaceae and strain DB0510T was distinctly separated from validly named genera of this family. Signature nucleotides in 16S rRNA gene sequence revealed that the strain cont...
Source: Archives of Microbiology - May 30, 2021 Category: Microbiology Authors: Jong Sung Lim Hyosun Lee Jigwan Son Song-Gun Kim Dong-Uk Kim Jong-Ok Ka Source Type: research

Efficiency of arsenic remediation from growth medium through Bacillus licheniformis modulated by phosphate (PO < sub > 4 < /sub > ) < sup > 3- < /sup > and nitrate (NO < sub > 3 < /sub > ) < sup > - < /sup > enrichment
Arch Microbiol. 2021 May 30. doi: 10.1007/s00203-021-02392-6. Online ahead of print.ABSTRACTBacillus licheniformis DAS-1 and DAS-2 were found as potent tool for removal/uptake of arsenic [As(V) and As(III)] and reduction [(As(V) to As(III)] of arsenic from the liquid growth medium in our previous studies. Present work gives light on modulation of arsenic remediation (in terms of uptake and reduction) by two selected essential soil nutrients, phosphate (PO4)3- and nitrate (NO3)-. PO43- has structural analogy with arsenate [AsO43-/As(V)] that compete with cell uptake of As(V). It was found that enrichment of 0.75 mM of PO43-...
Source: Archives of Microbiology - May 30, 2021 Category: Microbiology Authors: Kumari Tripti Shardendu Shardendu Source Type: research

Chemical composition and potentiating action of Norfloxacin mediated by the essential oil of Piper caldense C.D.C. against Staphylococcus aureus strains overexpressing efflux pump genes
This study had as objectives characterize the chemical composition of the essential oil from Piper caldense (EOPC), evaluate its potential antimicrobial activity, and investigate the synergistic effect with Norfloxacin against multidrug-resistant S. aureus overproducing efflux pumps, as well as, verify the EOPC ability to inhibit the Candida albicans filamentation. EOPC was extracted by hydrodistillation, and the chemical constituents were identified by gas chromatography, allowing the identification of 24 compounds (91.9%) classified as hydrocarbon sesquiterpenes (49.6%) and oxygenated sesquiterpenes (39.5%). Antimicrobia...
Source: Archives of Microbiology - May 30, 2021 Category: Microbiology Authors: Antonio Linkoln Alves Borges Leal Camila Fonseca Bezerra Camila Confortin Luiz Everson da Silva Emanuelle Machado Marinho M árcia Machado Marinho Mayron Alves Vasconcelos Terezinha Gon çalves da Silva Emmanuel Silva Marinho Alexandre Magno Rodrigues Tei Source Type: research

Enhanced production of prodigiosin by Serratia marcescens UCP 1549 using agrosubstrates in solid-state fermentation
Arch Microbiol. 2021 May 30. doi: 10.1007/s00203-021-02399-z. Online ahead of print.ABSTRACTThis work aimed to investigate the production of prodigiosin by S. marcescens UCP 1549 in solid-state fermentation (SSF), as a sustainable alternative for reducing the production costs and environmental impact. Thus, different agro-industrial substrates were used in the formulation of the prodigiosin production medium, obtaining the maximum yield of pigment (119.8 g/kg dry substrate) in medium consisting of 5 g wheat bran, 5% waste soybean oil and saline solution. The pigment was confirmed as prodigiosin by the maximum absorbance pe...
Source: Archives of Microbiology - May 30, 2021 Category: Microbiology Authors: Renata Andreia Dos Santos Dayana Montero Rodr íguez Lucas Albuquerque Rosendo da Silva S érgio Mendonça de Almeida Galba Maria de Campos-Takaki Marcos Ant ônio Barbosa de Lima Source Type: research