Marine macroalga-associated heterotroph Bacillus velezensis as prospective therapeutic agent.
Abstract Marine macroalgae and their accompanying microbial flora were proved to be the reservoir of potential bioactive compounds with promising pharmacological applications. Heterotrophic bacteria concomitant with the marine algae were isolated and screened for their antibacterial potential against clinically recognized pathogens. The bacterial isolate with greater bioactive properties was identified as Bacillus velezensis MBTDLP1 (phylum Firmicutes), which was isolated from the marine macroalga Laurencia papillosa, by integrated morphological, biochemical and molecular characterization. B. velezensis ...
Source: Archives of Microbiology - January 13, 2021 Category: Microbiology Authors: Francis A, Chakraborty K Tags: Arch Microbiol Source Type: research

Response of intercropped barley and fenugreek to mono- and co-inoculation with Sinorhizobium meliloti F42 and Variovorax paradoxus F310 under contrasting agroclimatic regions.
Abstract In the present research, we aimed to select efficient rhizobia and plant growth-promoting rhizobacteria (PGPR) from fenugreek nodules and assess their performance as bio-inoculum for intercropped fenugreek and barley. Inoculation effects with selected bacteria were investigated firstly on fenugreek plants under greenhouse experiment and secondly on intercropped fenugreek and barley under three different agro-environmental conditions for two consecutive years. Sinorhizobium meliloti F42 was selected due to its ability to nodulate fenugreek and effectively improve plant growth. Among non-nodulating endophyt...
Source: Archives of Microbiology - January 12, 2021 Category: Microbiology Authors: Toukabri W, Ferchichi N, Hlel D, Jadlaoui M, Kheriji O, Mhamdi R, Trabelsi D Tags: Arch Microbiol Source Type: research

Use of RNAi as a preliminary tool for screening putative receptors of nematicidal toxins from Bacillus thuringiensis.
tro MC, Ibarra JE Abstract Bacillus thuringiensis is a potential control agent for plant-parasitic nematodes. Nematode intestinal receptors for Cry21-type toxins are poorly known. Therefore, a strategy was tested as a primary screening tool to find possible Cry toxin receptors, using a nematicidal Bt strain and the RNAi technique on Caenorhabditis elegans. Six genes encoding intestinal membrane proteins were selected (abt-4, bre-1, bre-2, bre-3, asps-1, abl-1) as possible targets for Cry proteins. Fractions of each selected gene were amplified by PCR. Amplicons were cloned into the L4440 vector to transform the E....
Source: Archives of Microbiology - January 11, 2021 Category: Microbiology Authors: García-Montelongo M, González-Villarreal SE, Del Rincón-Castro MC, Ibarra JE Tags: Arch Microbiol Source Type: research

Comparative proteomic analysis of outer membrane vesicles from Brucella suis, Brucella ovis, Brucella canis and Brucella neotomae.
In this study, OMVs of Brucella suis, B. ovis, B. canis, and B. neotomae were purified and analyzed by SDS-PAGE, transmission electron microscopy and liquid chromatography coupled to mass spectrometry to determine the pan-proteome of these vesicles. In addition, antigenic proteins were detected by western blot with anti-Brucella sera. The in silico analysis of the pan-proteome revealed many homologous proteins, such as Omp16, Omp25, Omp31, SodC, Omp2a, and BhuA. Proteins contained in the vesicles from different Brucella species were detected by anti-Brucella sera. The occurrence of previously described immunogenic proteins...
Source: Archives of Microbiology - January 11, 2021 Category: Microbiology Authors: Socorro Ruiz-Palma MD, Avila-Calderón ED, Aguilera-Arreola MG, López-Merino A, Ruiz EA, Morales-García MDR, López-Villegas EO, Gomez-Lunar Z, Arellano-Reynoso B, Contreras-Rodríguez A Tags: Arch Microbiol Source Type: research

Distribution of sigma factors delineates segregation of virulent and avirulent Mycobacterium.
Abstract The genus Mycobacterium includes a wide range of species of both slow and rapid growth under major pathogens, opportunists, and saprophytes. The number and combination of sigma factors are extremely diversified among various species of Mycobacterium. The comparative genome analysis illustrates that SigC, SigD, SigG, SigH, SigK and SigI are dominant among the pathogens. Evolutionary analysis using Bayesian inference on 16S rRNA and MLST-based phylogeny using 14 housekeeping genes distinctly differentiate the slow-growing Mycobacterium from fast growers and segregate pathogens from opportunists and saprophy...
Source: Archives of Microbiology - January 11, 2021 Category: Microbiology Authors: Mallick Gupta A, Mandal S Tags: Arch Microbiol Source Type: research

Cabbage defense response provoked by Trichoderma Th-LAAO.
Abstract To investigate the molecular mechanism of Trichoderma L-amino acid oxidase (Th-LAAO) in protecting and in promoting growth of cabbage infected with Botrytis cinerea, a three-way interaction system was established. Cabbage leaves treated with purified Th-LAAO significantly constrained damaged leaf area caused by B. cinerea infection. In response to Th-LAAO treatment, the expression levels of genes involved in photosynthesis, such as ribulose-1,5-bisphosphate carboxylase oxygenase, Rubisco activase, and ATP synthase increased 2.54, 2.18, and 1.41 folds, respectively. The transcription levels of sucrose tran...
Source: Archives of Microbiology - January 11, 2021 Category: Microbiology Authors: Liu CM, Liu SY, Liao CK, Lo CT, Lin KC, Peng KC Tags: Arch Microbiol Source Type: research

Streptomyces cupreus sp. nov., an antimicrobial producing actinobacterium isolated from Himalayan soil.
Abstract A novel actinobacterium, designated Streptomyces sp strain PSKA01T, was isolated from a soil sample of Kashmir Himalaya, India (latitude:34˚-01'N; longitude:74˚-47'E; altitude: 5328 ft). The taxonomic position of this strain was revealed by a polyphasic approach. Comparative analysis of the 16S rRNA gene sequences indicated that PSKA01T was closely related to the members of the genus Streptomyces, with the highest similarity to Streptomyces venetus CMU-AB225T (99.3%), Streptomyces levis NBRC 15423 T (99.3%), Streptomyces misionensis DSM 40306 T (99.2%), Streptomyces carpinensis NRRL B-16921&nb...
Source: Archives of Microbiology - January 7, 2021 Category: Microbiology Authors: Maiti PK, Mandal S Tags: Arch Microbiol Source Type: research

Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants (PPR) virus in cell culture.
Abstract Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titration in Vero cells and the viral presence was confirmed by real-time RT-PCR. The virus was first detected in both the supernatants and cell pellets at 12 hpi. The virus titre reached its plateau at 72 hpi. Maximum virus titre of CAV was 6.2 log10 TCID50/ml and that of CFV was 5.2 log10 TCID50/...
Source: Archives of Microbiology - January 5, 2021 Category: Microbiology Authors: Siddiqui MSI, Islam MR, Chowdhury EH Tags: Arch Microbiol Source Type: research

Antibacterial, anti-biofilm and anti-quorum sensing activities of Artemisia dracunculus essential oil (EO): a study against Salmonella enterica serovar Typhimurium and Staphylococcus aureus.
Abstract The study evaluates the effect of Artemisia dracunculus essential oil (EO) on two pathogenic bacteria Salmonella enterica serovar Typhimurium and Staphylococcus aureus and Vero cell line. To evaluating the anti-biofilm potential of the EO, a microtiter-plate test (MtP) and scanning electron microscopy (SEM) were performed. The quorum-sensing inhibitory properties were examined by QS-related gene expression at sub-MIC concentrations of Artemisia dracunculus EO. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) test was used to determine the cytotoxicity potential of the EO aga...
Source: Archives of Microbiology - January 5, 2021 Category: Microbiology Authors: Mohammadi Pelarti S, Karimi Zarehshuran L, Babaeekhou L, Ghane M Tags: Arch Microbiol Source Type: research

Genome sequencing and functional annotation of Bacillus sp. strain BS-Z15 isolated from cotton rhizosphere soil having antagonistic activity against Verticillium dahliae.
Abstract In the present study, antagonistic activity of bacterial strain BS-Z15, was evaluated against Verticillium dahlia. The fermented broth of BS-Z15 inhibited the growth of Verticillium dahliae. The genome of strain BS-Z15 had a total size of 4,068,702 base pairs and contained 4318 genes, of which 4196 are coding sequences and 122 are non-coding RNA. Among these genes, nine genomic islands, 86 tRNAs, 13 sRNAs, and one prophage was determined. With the help of annotation databases, most unigene functions were identified. At the same time, genomic comparison between BS-Z15 and 12 Bacillus members showed that th...
Source: Archives of Microbiology - January 5, 2021 Category: Microbiology Authors: Chen ZY, Abuduaini X, Mamat N, Yang QL, Wu MJ, Lin XR, Wang R, Lin RR, Zeng WJ, Ning HC, Zhao HP, Li JY, Zhao HX Tags: Arch Microbiol Source Type: research

Biochemical changes in microalga Porphyridium cruentum associated with silver nanoparticles biosynthesis.
Abstract For the first time, the microalga Porphyridium cruentum was tested for its ability to produce silver nanoparticles. To characterize formed silver nanoparticles UV-vis Spectrometry, Scanning Electron Microscopy, Energy-dispersive analysis of X-rays and X-ray diffraction were used. It was shown that after biomass exposure to silver nitrate solution the extracellular formation of spherical-like nanoparticles took place. Functional groups responsible for metal binding were determined by Fourier-transform infrared spectroscopy. The complex of biochemical tests was used for biomass characterization and assessme...
Source: Archives of Microbiology - January 5, 2021 Category: Microbiology Authors: Cepoi L, Rudi L, Zinicovscaia I, Chiriac T, Miscu V, Rudic V Tags: Arch Microbiol Source Type: research

Assessment of the anti-pathogenic effects of condensed tannin extracts using scanning electron microscopy.
Abstract Two different types of condensed tannins (CTs), which were extracted and purified from tilia (Tilia L.) and black locust (Robinia pseudoacacia), were studied and tested against two kinds of bacteria, including Gram-negative and Gram-positive, avian pathogenic E. coli (APEC) and Staphylococcus epidermidis (S. epidermidis) respectively, by minimal bactericidal concentrations (MBCs) and scanning electron microscopy (SEM). Both CT extracts were significantly effective (p ≤ 0.05) at MBCs of 5-10 mg CT/ml against APEC (Gram-negative), and at 1.25-5 mg CT/ml on S. epidermidis (Gram-pos...
Source: Archives of Microbiology - January 5, 2021 Category: Microbiology Authors: Dakheel MM, Kaur A, Al-Mnaser AA, Mueller-Harvey I, Woodward MJ, Rymer C Tags: Arch Microbiol Source Type: research

Investigation on mycelial growth requirements of Cantharellus cibarius under laboratory conditions.
This study is the first report on the optimisation of in vitro growth requirements of C. cibarius. PMID: 33399895 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - January 5, 2021 Category: Microbiology Authors: Deshaware S, Marathe SJ, Bedade D, Deska J, Shamekh S Tags: Arch Microbiol Source Type: research

Myceligenerans indicum sp. nov., an actinobacterium isolated from mangrove sediment of Sundarbans, India.
Abstract A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem. The isolate has been characterized based on polyphasic approaches. The isolate exhibit well-developed substrate mycelia along with the presence of cocci- and rod-shaped elements. The organism can grow across a wide range of temperature, salinity, and pH as well as on different carbon sources. Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage). Presence of ketos...
Source: Archives of Microbiology - January 5, 2021 Category: Microbiology Authors: Asha K, Bhadury P Tags: Arch Microbiol Source Type: research

Reclassification of Sphaerotilus natans subsp. sulfidivorans Gridneva et al. 2011 as Sphaerotilus sulfidivorans sp. nov. and comparative genome analysis of the genus Sphaerotilus.
Abstract Filamentous iron oxides accumulating bacteria Sphaerotilus natans subsp. natans and S. natans subsp. sulfidivorans were described as subspecies based on 99.7% identity of their 16S rRNA sequences, in spite of important physiological difference. The ANI between their genomes was 94.7%, which indicate their assignment to different species. S. natans subsp. sulfidivorans and S. montanus possess genes for a complete SOX system, while S. natans subsp. natans encode only SoxYZ. There are genes for the Calvin cycle in the genomes of S. hippei DSM 566T, S. natans subsp. sulfidivorans D-501T, and S. montanus HST. ...
Source: Archives of Microbiology - January 5, 2021 Category: Microbiology Authors: Grabovich MY, Smolyakov DD, Beletsky AV, Mardanov AV, Gureeva MV, Markov ND, Rudenko TS, Ravin NV Tags: Arch Microbiol Source Type: research

Mesobacillus aurantius sp. nov., isolated from an orange-colored pond near a solar saltern.
Abstract An endospore producing, strict aerobic, Gram-stain-positive, orange-colored colony forming bacterium designated as strain JC1013T was isolated from an orange pond near a solar saltern of Tamil Nadu, India. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain was affiliated to the family Bacillaceae of the phylum Firmicutes. Strain showed highest 16S rRNA gene sequence identity of 98.7% with Mesobacillus selenatarsenatis SF-1 T and below 98.3% with other members of the genus Mesobacillus. Strain JC1013T produced carotenoid pigments and indole compounds. Major cellular fatty acids...
Source: Archives of Microbiology - January 4, 2021 Category: Microbiology Authors: Rai A, Smita N, Shabbir A, Jagadeeshwari U, Keertana T, Sasikala C, Ramana CV Tags: Arch Microbiol Source Type: research

Effect of fermentation bed on bacterial growth in the fermentation mattress material and cecum of ducks.
In this study, 16S rDNA sequencing technology was adopted to investigate the influence of fermentation bed on the cecum microorganisms of ducks. Two feeding density treatment groups were set up, including group A (n = 4brids/m2) and group B (n = 6brids/m2). Samples were collected from the intermediate core fermentation layer (10-20 cm) of the fermented mattress materials and from the intestinal contents of ducks at 4, 6 and 8 weeks, respectively. Results showed that Bacteroidetes (20.12-27.17%) and Ruminococcaceae UCG-014 (2.97-10.1%) were the predominant microorganisms in duck cecum, ...
Source: Archives of Microbiology - January 4, 2021 Category: Microbiology Authors: Wang JM, Gan XM, Pu FJ, Wang WX, Ma M, Sun LL, Hu JW, Hu B, Zhang RP, Bai LL, Li L, Liu HH Tags: Arch Microbiol Source Type: research

Puerhibacterium puerhi gen. nov., sp. nov., a novel member of the family Promicromonosporaceae, isolated from Pu-erh tea pile-fermentation.
Abstract A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China. Strain TLY-12T grew at 15-37 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 9.0) and 0-9.0% (w/v) NaCl (optimum, 3.0%). The major cellular fatty acids were anteiso-C15:0, C16:0 and iso-C16:0. The respiratory quinone were menaquinones MK-9 (H2) and MK-9 (H4). The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylinositol (PI), phosphoglycolipid (PGL), glycolipid (GL) and an unidentified phospholipid (PL...
Source: Archives of Microbiology - January 4, 2021 Category: Microbiology Authors: Yang RJ, Zhou, Wang QM, Han L, Peng WS, Liu L, Wang XJ, Yan L, Lv J, Sheng J Tags: Arch Microbiol Source Type: research

Quorum sensing systems, related virulence factors, and biofilm formation in Pseudomonas aeruginosa isolated from fish.
In conclusion, the high number of P. aeruginosa isolates from fish tested have two QS systems and related virulence factors. There was also correlation between them. PMID: 33398400 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - January 4, 2021 Category: Microbiology Authors: Sırıken B, Öz V, Erol İ Tags: Arch Microbiol Source Type: research

Phenotypic and genotypic detection of antibiotic-resistant bacteria in fresh fruit juices from a public hospital in Rio de Janeiro.
This study aimed to detect the presence of phenotypic and genotypic resistance in eight samples of fresh fruit juices served to patients admitted to a hospital in Rio de Janeiro. The growth of microorganisms on MacConkey and XLD agar was carried out to obtain a "pool" of Gram-negative bacteria. The disk diffusion test and the polymerase chain reaction were performed to detect the phenotypic and genotypic resistance of Gram-negative bacteria to the tested antibiotics. The multidrug resistance was detected in all samples and the shv, tem, ctx, tetA, tetB and oxa- 48 genes were found in the samples, including the pr...
Source: Archives of Microbiology - January 4, 2021 Category: Microbiology Authors: Trocado ND, de Moraes MS, Aveleda L, Silva CR, Marin VA Tags: Arch Microbiol Source Type: research

Genome analysis of Streptococcus salivarius subsp. thermophilus type strain ATCC 19258 and its comparison to equivalent strain NCTC 12958.
Abstract Streptococcus thermophilus is one of the lactic acid bacteria applied as the main starter for dairy foods. A type strain of Streptococcus salivarius subsp. thermophilus ATCC 19258 has been used in the genetic and biochemical characterization of their genes or gene products. While the genome sequence of NCTC 12958 as an equivalent to ATCC 19258 is available, characterization of whether both collections are identical remains to be validated. Here, we report the complete genome sequence of ATCC 19258, which contains one 2.1 Mb chromosome with a 39.0% of G + C content, and includes 2255 pro...
Source: Archives of Microbiology - January 4, 2021 Category: Microbiology Authors: Cho H, Park KE, Kim KS Tags: Arch Microbiol Source Type: research

Effect of Senecio scandens ethanol extract on gut microbiota composition in mice.
Abstract The gut microbiota inhabits the animal intestinal tract, and dysbiosis of the gut microbiota may result in disease. Senecio scandens has pharmaceutical antibacterial activities and is regarded as a broad-spectrum antibiotic in traditional Chinese medicine. Extracts of S. scandens are reported to show strong antimicrobial activity, and quercetin significantly decreases some species in the caecal microflora. However, the bactericidal effects of the extracts on the gut microbiota remain obscure. Here, we supplied ethanol extract of S. scandens, which might possibly be used as an alternative for chemical anti...
Source: Archives of Microbiology - January 4, 2021 Category: Microbiology Authors: Yao G, Zhang H, Luo G, Wang Z, Zhao H, Zhang J, Dong Y, Gao Y, Wu S Tags: Arch Microbiol Source Type: research

Genetic diversity and distribution of rhizobia associated with soybean in red soil in Hunan Province.
Abstract To explore the genetic diversity and distribution of rhizobia in the rhizosphere of soybean grown in red soil, we have collected 21 soil samples from soybean fields across seven counties in Hunan province, China. MiSeq sequencing of rpoB gene was used to determine the intra-species diversity of rhizobia existing in soybean rhizospheres. Soil chemical properties were determined by routine methods. The Principal Coordinates Analysis (PCoA) plot indicated a clear biogeographical pattern characterizing the soybean rhizosphere across different sites. The Mantel test demonstrated that biogeographical pattern wa...
Source: Archives of Microbiology - January 4, 2021 Category: Microbiology Authors: Liu L, Chen X, Hu S, Zhan Q, Peng W Tags: Arch Microbiol Source Type: research

Photoautotrophic cultivation of Chlamydomonas reinhardtii in open ponds of greenhouse.
In this study, the photoautotrophic growth of C. reinhardtii was evaluated in 1-m2 open ponds placed in greenhouse. Sodium bicarbonate (NaHCO3) was evaluated as an alternative buffering agent to tris. The effect of buffer and pH was tested. The growth was studied in the presence of various nitrogen [urea and ammonium bicarbonate (NH4HCO3)] sources. In the study, it was found that 125-ppm NaHCO3 as an optimum concentration. The buffering agent in the media was found to have major impact on growth. Without buffering agent, culture did not grow, and pH drop was observed. The sodium bicarbonate-buffered media reported to ...
Source: Archives of Microbiology - January 3, 2021 Category: Microbiology Authors: Chandrashekharaiah PS, Paul V, Kodgire S, Chawada H, Thorat R, Kushwaha S, Sanyal D, Dasgupta S Tags: Arch Microbiol Source Type: research

Citral and its derivatives inhibit quorum sensing and biofilm formation in Chromobacterium violaceum.
Abstract With an upsurge in multidrug resistant bacteria backed by biofilm defence armours, there is a desperate need of new antibiotics with a non-traditional mechanism of action. Targeting bacteria by misguiding them or halting their communication is a new approach that could offer a new way to combat the multidrug resistance problem. Quorum sensing is considered to be the achilles heel of bacteria that has a lot to offer. Since, both quorum sensing and biofilm formation have been related to drug resistance and pathogenicity, in this study we synthesised new derivatives of citral with antiquorum sensing and biof...
Source: Archives of Microbiology - January 3, 2021 Category: Microbiology Authors: Batohi N, Lone SA, Marimani M, Wani MY, Al-Bogami AS, Ahmad A Tags: Arch Microbiol Source Type: research

Evaluation of interaction among indigenous rhizobacteria and Vigna unguiculata on remediation of metal-containing abandoned magnesite mine tailing.
Abstract Abandoned magnesite mine heap causing pollution to nearby farmland and water reservoir. Thus the intention of this research was to screening metal mobilizing and absorbing bacteria from the rhizosphere section of V. unguiculata from farmland nearby to magnesite mine. Further, studied their stimulus effect on growth, biomass, and phytoextraction prospective of V unguiculata in mine tailing. The results of the physicochemical properties of mine tailing shows that four metals (Pb, Mn, Cd, and Zn) were crossing the permissible limit. Out of 27 isolates, 2 isolates (MMS15 and MMS17) were identified with maximu...
Source: Archives of Microbiology - January 3, 2021 Category: Microbiology Authors: Narayanan M, Ranganathan M, Kandasamy G, Kumarasamy S Tags: Arch Microbiol Source Type: research

Investigation of the effects of various stress factors on biofilms and planktonic bacteria in cooling tower model system.
In this study, 14 week-old biofilms were treated with temperatures of 4 °C, 65 °C; pH of 3, 11; 2 and 10 mg/l chlorine, 2 and 10 mg/l monochloramine; hypotonic salt (0.01% NaCl) and hypertonic salt (3% NaCl) solution. For enumeration, number of aerobic heterotrophic bacteria was determined by conventional culture method, number of live bacteria was determined by LIVE/DEAD viability kit, CTC-DAPI and Alamar blue staining methods. Temperature of 65 °C, pH of 3, 10 mg/l monochloramine and hypertonic salt solution were the most effective parameters for decontamination of biofilm a...
Source: Archives of Microbiology - January 3, 2021 Category: Microbiology Authors: Vatansever C, Turetgen I Tags: Arch Microbiol Source Type: research

Mechanism and application of Sesbania root-nodulating bacteria: an alternative for chemical fertilizers and sustainable development.
Abstract Chemical fertilizers are used in large-scale throughout the globe to satisfy the food and feed requirement of the world. Demanding cropping with the enhanced application of chemical fertilizers, linked with a decline in the recycling of natural or other waste materials, has led to a decrease in the organic carbon levels in soils, impaired soil physical properties and shrinking soil microbial biodiversity. Sustenance and improvement of soil fertility are fundamental for comprehensive food security and ecological sustainability. To feed the large-scale growing population, the role of biofertilizers and thei...
Source: Archives of Microbiology - January 3, 2021 Category: Microbiology Authors: Singh K, Gera R, Sharma R, Maithani D, Chandra D, Bhat MA, Kumar R, Bhatt P Tags: Arch Microbiol Source Type: research

Paenibacillus puerhi sp. nov., isolated from the rhizosphere soil of Pu-erh tea plants (Camellia sinensis var. assamica).
Abstract An aerobic, Gram-staining-positive, rod-shaped, endospore-forming and motile bacterial strain, designated SJY2T, was isolated from the rhizosphere soil of tea plants (Camellia sinensis var. assamica) collected in the organic tea garden of the Jingmai Pu-erh tea district in Pu'er city, Yunnan, southwest China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Paenibacillus. The closest phylogenetic relative was Paenibacillus filicis DSM 23916T (98.1% similarity). The major fatty acids (> 10% of the total fatty acids) were anteiso-C15:0 and isoC16...
Source: Archives of Microbiology - January 2, 2021 Category: Microbiology Authors: Yang RJ, Zhou, Wang QM, Wang XH, Zhang WJ, Zhuang L, Wang XJ, Yan L, Lv J, Sheng J Tags: Arch Microbiol Source Type: research

Hongsoonwoonella zoysiae gen. nov., sp. nov., a new member of the family Stappiaceae isolated from a tidal mudflat.
Abstract A Gram stain-negative bacterial strain, designated SY4-7T, was isolated from rhizosphere mudflat of a halophyte (Zoysia sinica) collected around Seonyu Island, Republic of Korea. Cells of the organism were strictly aerobic, non-sporulating, non-motile rods and grew at 20-42 °C, pH 6-8 and 1-6% (w/v) NaCl. The 16S rRNA gene-based phylogenetic analyses revealed that strain SY4-7T formed an independent cluster separated from the recognized genera of the family Stappiaceae, which was also supported by phylogenomic analysis-based 92-core gene sequences. The type stains of the phylogenetically closest ...
Source: Archives of Microbiology - January 2, 2021 Category: Microbiology Authors: Lee SD, Kim SM, Yang HL, Byeon YS, Kim IS Tags: Arch Microbiol Source Type: research

Phytoactinopolyspora limicola  sp. nov., an alkaliphilic actinomycete isolated from a soda alkali-saline soil.
Phytoactinopolyspora limicola sp. nov., an alkaliphilic actinomycete isolated from a soda alkali-saline soil. Arch Microbiol. 2021 Jan 02;: Authors: Wei W, Wang K, Hu X, Yang S, Zeng C, Hou Z, Liu S, Cui H, Zhu L Abstract An alkaliphilic actinomycete, designated HAJB-30 T, was isolated from a soda alkali-saline soil in Heilongjiang, Northeast China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HAJB-30 T was most closely related to type strains of the genus Phytoactinopolyspora with sequence similarities ranging between 93.5 and 98.9%. Strain HAJB-30 T g...
Source: Archives of Microbiology - January 2, 2021 Category: Microbiology Authors: Wei W, Wang K, Hu X, Yang S, Zeng C, Hou Z, Liu S, Cui H, Zhu L Tags: Arch Microbiol Source Type: research

Volatile organic compounds profile synthesized and released by endophytes of tomato (Solanum lycopersici L.) and their antagonistic role.
ti PA Abstract The endophytic microbiome uses mechanisms such as the secretion of diffusible antibiotic molecules, synthesis and release of volatile organic compounds, and/or toxins to protect plants. The aim of this research was to study the volatile organic compounds (VOCs) profile as well as the diffusible secondary metabolites produced and released by endophytic bacteria isolated from tomato plants that in in-vitro assays prevented growth of pathogenic fungi. Bacteria belonging to seven genera (Acinetobacter, Arthrobacter, Bacillus, Microbacterium, Pantoea, Pseudomonas, and Stenotrophomonas) were isolated from...
Source: Archives of Microbiology - January 2, 2021 Category: Microbiology Authors: López SMY, Pastorino GN, Balatti PA Tags: Arch Microbiol Source Type: research

Coniochaeta fungus benefits from its intracellular bacteria to form biofilm and defend against other fungi.
In this study, the likelihood of intracellular origin of bacteria as well as interaction of two microorganisms was assessed. Fluorescence and electron microscopy showed occurrence of several bacterial cells in fungal cytoplasm. A thick biofilm was observed on the surface of co-culture compared with thin one on bacterial and none on fungal monocultures. Field emission scanning electron microscopy (FESEM) micrographs of co-culture showed a dense network of fungal and bacterial cells embedded in a slime-like layer. Dual cultures revealed antagonistic activity of both fungus and bacteria against three Candida species. These fi...
Source: Archives of Microbiology - January 2, 2021 Category: Microbiology Authors: Heydari S, Siavoshi F, Sarrafnejad A, Malekzadeh R Tags: Arch Microbiol Source Type: research

Genome analysis of Salmonella phage vB_SalM_8-19 (genus Rosemountvirus).
Abstract This work describes the characterization and genome annotation of Salmonella phage vB_SalM_8-19 (referred to as 8-19) isolated from sewage samples collected in a pig farm in Jilin, China. This phage was capable of infecting 60% Salmonella strains in our lab stock. The genome of phage 8-19 is composed of linear double-stranded DNA that is 52,648 bp in length with a G + C content of 46.02%; containing 74 ORFs and no tRNA genes. In October 2019, phylogenetic analyses indicated that phage 8-19 might belong to a novel cluster among the other similar phages which have not been specifically cl...
Source: Archives of Microbiology - January 2, 2021 Category: Microbiology Authors: Cong C, Bing Dong W, Hui Jing C, Yu Yu Y, Yong Ping X, Li Li W, Shu Ying L, Ji Bin L, Mu X, Xiao Yu L Tags: Arch Microbiol Source Type: research

Assessment of the bioprotective potential of lactic acid bacteria against Listeria monocytogenes in ground beef.
In this study, the antilisterial compounds produced by Enterococcus isolates from the Patagonian environment and their effectiveness for the control of Listeria monocytogenes in a food model were studied. Enterococcus isolates whose cell-free supernatant presented activity against Listeria monocytogenes were identified and evaluated for their virulence factors. The activity of the antimicrobial compounds produced by Enterococcus sp. against Listeria monocytogenes Scott A in meat gravy and ground beef during refrigerated storage was tested. The results indicated that ten Enterococcus isolates presented activity against List...
Source: Archives of Microbiology - January 2, 2021 Category: Microbiology Authors: Gomez JS, Parada RB, Vallejo M, Marguet ER, Bellomio A, Perotti N, de Carvalho KG Tags: Arch Microbiol Source Type: research

In vitro evaluation of lysozyme activity and antimicrobial effect of extracts from four Tunisian lichens: Diploschistes ocellatus, Flavoparmelia caperata, Squamarina cartilaginea and Xanthoria parietina.
This study demonstrated that the investigated species are a potential source of bioactive compounds which are potentially important antimicrobial agents. PMID: 33388791 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - January 2, 2021 Category: Microbiology Authors: Mendili M, Essghaier B, Seaward MRD, Khadhri A Tags: Arch Microbiol Source Type: research

Thermoplasmata and Nitrososphaeria as dominant archaeal members in acid mine drainage sediment of Malanjkhand Copper Project, India.
Abstract Acid mine drainage (AMD) harbors all three life forms in spite of its toxic and hazardous nature. In comparison to bacterial diversity, an in-depth understanding of the archaeal diversity in AMD and their ecological significance remain less explored. Archaeal populations are known to play significant roles in various biogeochemical cycles within the AMD ecosystem, and it is imperative to have a deeper understanding of archaeal diversity and their functional potential in AMD system. The present study is aimed to understand the archaeal diversity of an AMD sediment of Malanjkhand Copper Project, India ...
Source: Archives of Microbiology - January 2, 2021 Category: Microbiology Authors: Gupta A, Saha A, Sar P Tags: Arch Microbiol Source Type: research

Detection and characterization of Enterobacteriaceae family members carried by commensal Rattus norvegicus from Tehran, Iran.
Abstract Wild rats are known to carry different microorganisms and are considered a reservoir of zoonotic pathogens worldwide. The urban rats were collected from five districts of Tehran and Gram-negative bacteria (GNB) were isolated from fecal samples and were identified using classical biochemical tests. The antibiotic susceptibility patterns of isolated bacteria were determined by Kirby-Bauer disk diffusion method, the results of which were interpreted in line with CLSI guideline. The frequency of antibiotic-resistant genes was identified using multiplex-PCR. Moreover, PCR method was used to identify the freque...
Source: Archives of Microbiology - January 1, 2021 Category: Microbiology Authors: Azimi T, Azimi L, Fallah F, Pourmand MR, Ostadtaghizadeh A, Abai MR, Rahimi Foroushani A Tags: Arch Microbiol Source Type: research

Correction to: Pontibacter fetidus sp. nov. and Pontibacter burrus sp. nov., isolated from the soil.
PMID: 33367946 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - December 26, 2020 Category: Microbiology Authors: Park Y, Maeng S, Han JH, Lee SE, Chang Y, Kim MK Tags: Arch Microbiol Source Type: research

Alkaline lipase production by novel meso-tolerant psychrophilic Exiguobacterium sp. strain (AMBL-20) isolated from glacier of northeastern Pakistan.
This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The isolated bacterium was identified as Exiguobaterium sp. strain AMBL-20T on the basis of morphological, biochemical, and phylogenetic analysis of 16S rRNA sequences with GenBank accession number MW229267. The bacterial strain was further screened for its lipolytic activity, biosynthesis, and characterization by different parameters with the aim of maximizing lipase activity. Results showed that 2% Olive oi...
Source: Archives of Microbiology - December 15, 2020 Category: Microbiology Authors: Yasin MT, Ali Y, Ahmad K, Ghani A, Amanat K, Basheir MM, Faheem M, Hussain S, Ahmad B, Hussain A, Bokhari SAI Tags: Arch Microbiol Source Type: research

Questioning the radiation limits of life: Ignicoccus hospitalis between replication and VBNC.
Abstract Radiation of ionizing or non-ionizing nature has harmful effects on cellular components like DNA as radiation can compromise its proper integrity. To cope with damages caused by external stimuli including radiation, within living cells, several fast and efficient repair mechanisms have evolved. Previous studies addressing organismic radiation tolerance have shown that radiotolerance is a predominant property among extremophilic microorganisms including (hyper-) thermophilic archaea. The analysis of the ionizing radiation tolerance of the chemolithoautotrophic, obligate anaerobic, hyperthermophilic Crenarc...
Source: Archives of Microbiology - December 15, 2020 Category: Microbiology Authors: Koschnitzki D, Moeller R, Leuko S, Przybyla B, Beblo-Vranesevic K, Wirth R, Huber H, Rachel R, Rettberg P Tags: Arch Microbiol Source Type: research

Pseudidiomarina piscicola sp. nov., isolated from cultured European seabass, Dicenthrarchus labrax.
te MJ Abstract Strain CECT 9734 T, a Gram-negative, aerobic, chemoorganotrophic bacterium, motile by polar flagella, was isolated from cultured European seabass, Dicenthrarchus labrax, in Spain. It grows from 5 to 42 ºC, 6-9 pH and 1-12% total salinity. Major cellular fatty acids are C15:0 iso, summed feature 9 (C17:1 iso w9c/C16:0 10-methyl) and C17:0 iso. The genome size is 2.5 Mbp and G + C content is 49.5 mol%. Comparative analysis of the 16S rRNA gene sequence shows that the strain is a member of Pseudidiomarina, with highest similarities with Pseudidiomarina halophila (97.0%) and...
Source: Archives of Microbiology - December 7, 2020 Category: Microbiology Authors: Macián MC, Lucena T, Arahal DR, Ruvira MA, Aznar R, Pujalte MJ Tags: Arch Microbiol Source Type: research

Siderophore production by Bacillus subtilis MF497446 and Pseudomonas koreensis MG209738 and their efficacy in controlling Cephalosporium maydis in maize plant.
Abstract Late wilt disease, caused by Cephalosporium maydis in maize plant, is one of the main economical diseases in Egypt. Therefore, to cope with this problem, we investigated the potentiality of plant growth promoting rhizobacteria in controlling this disease. Six strains (Bacillus subtilis, B. circulance, B. coagulanse, B. licheniformis, Pseudomonas fluroscence and P. koreensis) were screened for siderophore production, and using dual plate culture method and greenhouse experiment, antagonistic activity against C. maydis was studied. Using two superior strains, single and dual inoculation treatments in maize ...
Source: Archives of Microbiology - November 24, 2020 Category: Microbiology Authors: Ghazy N, El-Nahrawy S Tags: Arch Microbiol Source Type: research

Assessment of fungal diversity in soil rhizosphere associated with Rhazya stricta and some desert plants using metagenomics.
This study aimed to compare the fungal rhizosphere communities of Rhazya stricta, Enneapogon desvauxii, Citrullus colocynthis, Senna italica, and Zygophyllum simplex, and the gut mycobiota of Poekilocerus bufonius (Orthoptera, Pyrgomorphidae, "Usherhopper"). A total of 164,485 fungal reads were observed from the five plant rhizospheres and Usherhopper gut. The highest reads were in S. italica rhizosphere (29,883 reads). Species richness in the P. bufonius gut was the highest among the six samples. Ascomycota was dominant in all samples, with the highest reads in E. desvauxii (26,734 reads) rhizosphere. Sordariomy...
Source: Archives of Microbiology - November 24, 2020 Category: Microbiology Authors: Noor SO, Al-Zahrani DA, Hussein RM, Baeshen MN, Moussa TAA, Abo-Aba SM, Al-Hejin AM, Baeshen NA, Huelsenbeck JP Tags: Arch Microbiol Source Type: research

Soil bacterial community composition and diversity respond to soil environment in the Ebinur Lake Wetland.
Abstract To understand the relationship between the community structure of bacteria and soil environment, the diversity and composition of soil bacterial communities were investigated, based on 16S rRNA gene clone library, in three different sampling sites (SP1, SP2 and SP3) in the Bortala and Jinghe River basins of Ebinur Lake Wetland. The results showed that the diversity of bacteria among plots was SP2 > SP3 > SP1, and the richness was SP3 > SP2 > SP1. Community composition analysis of bacteria showed that Proteobacteria and Bacteroides account...
Source: Archives of Microbiology - November 23, 2020 Category: Microbiology Authors: He S, Hu W, Jin X, Han J Tags: Arch Microbiol Source Type: research

Identification of genes involved in phosphate solubilization and drought stress tolerance in chickpea symbiont Mesorhizobium ciceri Ca181.
Abstract Chickpea plant root colonizing bacteria Mesorhizobium ciceri Ca181 promotes plant growth and development through symbiotic association with root nodules. The potentially beneficial effects on plants generated due to this bacterium are mineral nutrient solubilization, abiotic stress tolerance, and nitrogen-fixation, though the molecular mechanisms underlying these probiotic capacities are still largely unknown. Hence, this study aims to describe the molecular mechanism of M. ciceri Ca181 in drought stress tolerance and phosphorus solubilization. Here we have developed the transposon inserted mutant library...
Source: Archives of Microbiology - November 23, 2020 Category: Microbiology Authors: Yadav A, Singh RP, Singh AL, Singh M Tags: Arch Microbiol Source Type: research

1,4-Naphthoquinone accumulates reactive oxygen species in Staphylococcus aureus: a promising approach towards effective management of biofilm threat.
Abstract Staphylococcus aureus, a Gram-positive opportunistic microorganism, promotes pathogenicity in the human host through biofilm formation. Microorganisms associated with biofilm often exhibit drug-resistance property that poses a major threat to public healthcare. Thus, the exploration of new therapeutic approaches is the need of the hour to manage biofilm-borne infections. In the present study, efforts are put together to test the antimicrobial as well as antibiofilm activity of 1,4-naphthoquinone against Staphylococcus aureus. The result showed that the minimum bactericidal concentration (MBC) of this comp...
Source: Archives of Microbiology - November 23, 2020 Category: Microbiology Authors: Paul P, Chakraborty P, Chatterjee A, Sarker RK, Dastidar DG, Kundu T, Sarkar N, Das A, Tribedi P Tags: Arch Microbiol Source Type: research

Comparison of different hypervariable regions of 16S rRNA for taxonomic profiling of vaginal microbiota using next-generation sequencing.
Abstract The exploration of vaginal microbiota by using next-generation sequencing (NGS) of 16S ribosomal RNA (rRNA) gene is widely used. Up to now, different hypervariable regions have been selected to study vaginal microbiota by NGS and there is no standard method for analysis. The study aimed to characterize vaginal microbiota from clinical samples using NGS targeting the 16S rRNA gene and to determine the performance of individual and concatenated hypervariable region sequences to generate the taxonomic profiles of the vaginal microbiota. Fifty-one vaginal DNA samples were subjected to 16S rRNA gene NGS based ...
Source: Archives of Microbiology - November 22, 2020 Category: Microbiology Authors: Sirichoat A, Sankuntaw N, Engchanil C, Buppasiri P, Faksri K, Namwat W, Chantratita W, Lulitanond V Tags: Arch Microbiol Source Type: research

Multilocus sequence typing of pathogenic Mycoplasma mycoides subsp. capri reveals the predominance of a novel clonal complex among isolates from goats in India.
Abstract Mycoplasma mycoides subsp. capri (Mmc) typically causes pneumonia, mastitis, arthritis, keratitis and septicaemia in goats. Mortality associated with Mmc in goat flocks is lower compared to Mycoplasma capricolum subsp. capripneumoniae-associated respiratory infections. Case fatality rates associated with Mmc ranged from 9.8 to 26.8% among several states in India. Molecular epidemiology approaches aimed at genotyping help to identify the diversity of isolates involved in a disease. Ten clinical pathogenic Mmc isolates were analysed by multilocus sequence typing (MLST) for studying genotypic relat...
Source: Archives of Microbiology - November 19, 2020 Category: Microbiology Authors: Subbaiyan A, Thomas P, Sankar M, Rana R, Chaudhuri P Tags: Arch Microbiol Source Type: research

Zinc- and cadmium-tolerant endophytic bacteria from Murdannia spectabilis (Kurz) Faden. studied for plant growth-promoting properties, in vitro inoculation, and antagonism.
Abstract This research aims to isolate and identify Zn- and Cd-tolerant endophytic bacteria from Murdannia spectabilis, identify their properties with and without Zn and Cd stress, and to investigate the effect of bacterial inoculation in an in vitro system. Twenty-four isolates could survive on trypticase soya agar (TSA) supplemented with Zn (250-500 mg L-1) and/or Cd (20-50 mg L-1) that belonged to the genera Bacillus, Pantoea, Microbacterium, Curtobacterium, Chryseobacterium, Cupriavidus, Siphonobacter, and Pseudomonas. Each strain had different indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carbox...
Source: Archives of Microbiology - November 18, 2020 Category: Microbiology Authors: Rattanapolsan L, Nakbanpote W, Sangdee A Tags: Arch Microbiol Source Type: research