The gut bacterial flora associated with brown planthopper is affected by host rice varieties.
In this study, we investigated the gut bacterial communities in two BPH populations feeding on susceptible and resistant rice varieties by high-throughput amplicon sequencing. Our results revealed that the gut bacterial communities in BPH were species diverse. A total of 29 phyla and 367 genera were captured, with Proteobacteria and Acinetobacter being the most prominent phylum and genus, respectively. Comparative analysis showed that significant differences in the profile of gut bacterial communities existed between the two BPH populations. The species richness detected in the population feeding on the resistant rice vari...
Source: Archives of Microbiology - September 17, 2020 Category: Microbiology Authors: Wang ZL, Pan HB, Wu W, Li MY, Yu XP Tags: Arch Microbiol Source Type: research
Shigella sonnei: virulence and antibiotic resistance.
Abstract Shigella sonnei is the emerging pathogen globally, as it is the second common infectious species of shigellosis (bloody diarrhoea) in low- and middle-income countries (LMICs) and the leading one in developed world. The multifactorial processes and novel mechanisms have been identified in S. sonnei, that are collectively playing apart a substantial role in increasing its prevalence, while replacing the S. flexneri and other Gram-negative gut pathogens niche occupancy. Recently, studies suggest that due to improvement in sanitation S. sonnei has reduced cross-immunization from Plesiomonas shigelliodes (havi...
Source: Archives of Microbiology - September 14, 2020 Category: Microbiology Authors: Shad AA, Shad WA Tags: Arch Microbiol Source Type: research
Draft genome sequence of Lactobacillus plantarum strain DMR17 isolated from homemade cow dahi of Sikkim Himalayan region: an evaluation of lactate fermentation and secondary metabolism.
Abstract Lactobacillus plantarum DMR17 was isolated from homemade cow dahi of Sikkim Himalayan region of India. Here, we report the draft genome sequence of this strain. A total of 21,176,638 paired-end reads were obtained which were assembled into 65 contigs. The reference genome used was L. plantarum WCFS1. The genome size of DMR17 was 3,228,341 bp with G + C content of 46.25%. 3302 coding sequences were predicted including 68 tRNA and 67 rRNA genes. More than 88% of the total pre-processed reads from the sample were mapped to the reference genome. The identified coding proteins were classifie...
Source: Archives of Microbiology - September 14, 2020 Category: Microbiology Authors: Tirwa RK, Najar IN, Thakur N, Chaurasia LK, Tamang B Tags: Arch Microbiol Source Type: research
Sulfurimicrobium lacus gen. nov., sp. nov., a sulfur oxidizer isolated from lake water, and review of the family Sulfuricellaceae to show that it is not a later synonym of Gallionellaceae.
Abstract A facultatively anaerobic sulfur-oxidizing bacterium, strain skT11T, was isolated from anoxic lake water of a stratified freshwater lake. As electron donor for chemolithoautotrophic growth, strain skT11T oxidized thiosulfate, tetrathionate, and elemental sulfur under nitrate-reducing conditions. Oxygen-dependent growth was observed under microoxic conditions, but not under fully oxygenated conditions. Growth was observed at a temperature range of 5-37 °C, with optimum growth at 28 °C. Strain skT11T grew at a pH range of 5.1-7.5, with optimum growth at pH 6.5-6.9. Heterotrophic growth was...
Source: Archives of Microbiology - September 14, 2020 Category: Microbiology Authors: Kojima H, Kanda M, Umezawa K, Fukui M Tags: Arch Microbiol Source Type: research
Pollution shapes the microbial communities in river water and sediments from the Olifants River catchment, South Africa.
wan D Abstract Human activities such as agriculture and mining are leading causes of water pollution worldwide. Individual contaminants are known to negatively affect microbial communities. However, the effect of multifaceted pollution on these communities is less well understood. We investigated, using next-generation sequencing of the 16S rRNA genes, the effects of multisource (i.e., fertilizer industry and mining) chronic pollution on bacterial and archaeal communities in water and sediments from the Olifants River catchment, South Africa. Water samples showed less microbial species diversity than sediments and...
Source: Archives of Microbiology - September 13, 2020 Category: Microbiology Authors: Valverde A, Cason ED, Gómez-Arias A, Bozkale D, Govender D, Riddell E, Cowan D Tags: Arch Microbiol Source Type: research
Lysobacter telluris sp. nov., isolated from Korean rhizosphere soil.
Abstract A Gram-stain-negative, aerobic, non-motile, non-spore-forming light-yellow-coloured rod-shaped bacterial strain, designated YJ15T, was isolated from soil at Bigeum island in Korea. Growth was observed at 10-37 °C (optimum, 28 °C), at pH 6.0-7.5 (optimum, pH 7.0) and in the absence of NaCl. Based on 16S rRNA gene sequence analysis, strain YJ15T was closely related to 'Lysobacter tongrenensis' YS037T (97.8%), Lysobacter pocheonensis Gsoil193T (96.5%) and Lysobacter daecheongensis Dae08T (95.8%) and phylogenetically grouped together with 'Lysobacter tongrenensis' YS037T, Lysobacter dokdonen...
Source: Archives of Microbiology - September 12, 2020 Category: Microbiology Authors: Lee JC, Whang KS Tags: Arch Microbiol Source Type: research
Visualization and characterization of Enterococcus faecalis biofilm structure in bovine dentin using 2D and 3D microscopic techniques.
This study showed that bacterial biofilm on dentin could be characterized by micro-CT after barium sulfate staining without causing any significant side effect on viable and total cell densities. PMID: 32918096 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - September 11, 2020 Category: Microbiology Authors: Keleş A, Keskin C, Kalkan M, Yakupoğulları Y, Gül M, Aydemir H, Şahin F Tags: Arch Microbiol Source Type: research
Genomic analysis of facultatively oligotrophic haloarchaea of the genera Halarchaeum, Halorubrum, and Halolamina, isolated from solar salt.
In this study, Halarchaeum sp. CBA1220, Halorubrum sp. CBA1229, and Halolamina sp. CBA1230, which are facultatively oligotrophic haloarchaea, were isolated from solar salt by culture under oligotrophic culture conditions. The complete genomes of strains CBA1220, CBA1229, and CBA1230 were sequenced and were found to contain 3,175,875, 3,582,278, and 3,465,332 bp, with a G + C content of 68.25, 67.66, and 66.75 mol %, respectively. In total, 60, 36, and 33 carbohydrate-active enzyme genes were determined in the respective strains. The strains harbored various genes encoding stress-tolerance prote...
Source: Archives of Microbiology - September 11, 2020 Category: Microbiology Authors: Lee C, Song HS, Lee SH, Kim JY, Rhee JK, Roh SW Tags: Arch Microbiol Source Type: research
Gene expressing analysis indicates the role of Pyrogallol as a novel antibiofilm and antivirulence agent against Acinetobacter baumannii.
Abstract Acinetobacter baumannii has emerged worldwide as a leading cause of hospital-acquired infections. Although A. baumannii was initially regarded to as a low-grade pathogen, evidence has been accumulated suggesting that A. baumannii infections are associated with increased mortality in critically ill patients. Here, we describe the efficacy of pyrogallol, a polyphenolic organic compound found in the galls and barks of various trees, which shows anti-biofilm and anti-virulence potential against A. baumannii. Pyrogallol shows concentration-based biofilm inhibition, as evidenced through...
Source: Archives of Microbiology - September 11, 2020 Category: Microbiology Authors: Abirami G, Durgadevi R, Velmurugan P, Ravi AV Tags: Arch Microbiol Source Type: research
Streptomyces boncukensis sp. nov., isolated from saltern soil.
Abstract A novel Gram-stain positive, aerobic, non-motile Actinobacterium, designated strain SB3404T, was isolated from saltern soil collected from Boncuk Saltern, Sungurlu-Çorum, Turkey, and subjected to a polyphasic taxonomic approach. The organism has shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis of strain SB3404T showed that it is closely related to Streptomyces albus NBRC 13014T (97.2% sequence similarity), Streptomyces xishensis YIM M 10378T (96.7%) and Streptomyces abyssal...
Source: Archives of Microbiology - September 11, 2020 Category: Microbiology Authors: Tatar D, Veyisoglu A, Saygin H, Sahin N Tags: Arch Microbiol Source Type: research
The dsRNA mycovirus ChNRV1 causes mild hypervirulence in the fungal phytopathogen Colletotrichum higginsianum.
mpo S Abstract The genus Colletotrichum comprises a large number of filamentous fungi responsible for anthracnose diseases in many tropical and subtropical fruits and vegetables. In particular, Colletotrichum higginsianum infects Brassicaceae species, including Arabidopsis. The C. higginsianum strain IMI349063A is naturally infected with a dsRNA virus, named Colletorichum higginsianum non-segmented virus (ChNRV1). Here, we investigated the biological effect of ChNRV1 in C. higginsianum by comparing strains with and without the virus. ChNRV1 does not have an effect on C. higginsianum growth under salt and cell-wall...
Source: Archives of Microbiology - September 10, 2020 Category: Microbiology Authors: Olivé M, Campo S Tags: Arch Microbiol Source Type: research
Bradyrhizobium campsiandrae sp. nov., a nitrogen-fixing bacterial strain isolated from a native leguminous tree from the Amazon adapted to flooded conditions.
ra FM Abstract The nitrogen-fixing bacterial strain UFLA 01-1174T was isolated from nodules of Campsiandra laurilifolia Benth. originating from the Amazon region, Brazil. Its taxonomic position was defined using a polyphasic approach. Analysis of the 16S rRNA gene placed the strain in the Bradyrhizobium genus, the closest species being B. guangdongense CCBAU 51649T and B. guangzhouense CCBAU 51670T, both with 99.8% similarity. Multilocus sequence analysis (MLSA) of recA, gyrB, glnII, rpoB, atpD, and dnaK indicated that UFLA 01-1174T is a new species, most closely related to B. stylosanthis BR 446T (94.4%) and...
Source: Archives of Microbiology - August 28, 2020 Category: Microbiology Authors: Cabral Michel D, Martins da Costa E, Azarias Guimarães A, Soares de Carvalho T, Santos de Castro Caputo P, Willems A, de Souza Moreira FM Tags: Arch Microbiol Source Type: research
Vibrio tetraodonis sp. nov.: genomic insights on the secondary metabolites repertoire.
on CC Abstract Description of a Gram-negative, motile, circular-shaped bacterial strain, designated A511T obtained from the skin of the pufferfish Sphoeroides spengleri (Family Tetraodontidae), collected in Arraial do Cabo, Brazil. Optimum growth occurs at 20-28 °C in the presence of 3% NaCl. The genome sequence of the novel isolate consisted of 4.36 Mb, 3,976 coding genes and G + C content of 42.5%. Genomic taxonomy analyses based on average amino acid (AAI), genome-to-genome-distance (GGDH) and phylogenetic reconstruction placed A511T (= CBAS 712T = CAIM 1939T) in...
Source: Archives of Microbiology - August 25, 2020 Category: Microbiology Authors: Azevedo GPR, Mattsson HK, Lopes GR, Vidal L, Campeão M, Tonon LAC, Garcia GD, Tschoeke DA, Silva BS, Otsuki K, Gomez-Gil B, Swings J, Thompson FL, Thompson CC Tags: Arch Microbiol Source Type: research
Composition and predicted functions of the bacterial community in spouting pool sediments from the El Tatio Geyser field in Chile.
This study provides fundamentally important information on the structure and function predictive functions of microbiota communities in spouting pools. Moreover, since the ETGF is intensively visited and impacted by tens of thousands of tourists every year, our results can be used to help guide the design of sustainable conservation strategies. PMID: 32816051 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - August 20, 2020 Category: Microbiology Authors: Zhang Q, Campos M, Larama G, Acuña JJ, Valenzuela B, Solis F, Zamorano P, Araya R, Sadowsky MJ, Jorquera MA Tags: Arch Microbiol Source Type: research
In silico analyses of the genomes of three new bacteriocin-producing bacteria isolated from animal's faeces.
Abstract Here, we have analysed and explored the genome sequences of three newly isolated bacteria that were recently characterised for their probiotic activities and ability to produce bacteriocins. These strains, isolated from faeces of animals living in captivity at the zoological garden of Lille (France), are Escherichia coli ICVB443, Enterococcus faecalis ICVB501 and Pediococcus pentosaceus ICVB491. Their genomes have been analysed and compared to those of their pathogenic or probiotic counterparts. The genome analyses of E. coli ICVB443 and Ent. faecalis ICVB501 displayed similarities to those of probiotics ...
Source: Archives of Microbiology - August 17, 2020 Category: Microbiology Authors: Eveno M, Belguesmia Y, Bazinet L, Gancel F, Fliss I, Drider D Tags: Arch Microbiol Source Type: research
Antibiotic-resistant Enterobacteriaceae from diseased freshwater goldfish.
This study is the first of its kind to investigate the antimicrobial resistance profile of enterobacteriaceae recovered from goldfish, before and after plasmid curing. PMID: 32803348 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - August 17, 2020 Category: Microbiology Authors: Preena PG, Dharmaratnam A, Raj NS, Raja SA, Nair RR, Swaminathan TR Tags: Arch Microbiol Source Type: research
Plasmid stability of potential probiotic Lactobacillus plantarum strains in artificial gastric juice, at elevated temperature, and in the presence of novobiocin and acriflavine.
In this study, the presence of plasmids responsible for carbohydrate fermentation and antibiotic resistance and the stability of these plasmids in artificial gastric juice were investigated in 20 Lactobacillus plantarum strains with probiotic properties. Plasmid curing was performed with novobiocin, acriflavine and elevated incubation temperature to identify plasmids encoded with carbohydrate fermentation and antibiotic resistance genes and to compare them with artificial gastric juice. Plasmid profiling of the strains revealed that 100% of the strains were harbouring plasmids in varying sizes and numbers. The plasmid numb...
Source: Archives of Microbiology - August 16, 2020 Category: Microbiology Authors: Sağlam H, Karahan AG Tags: Arch Microbiol Source Type: research
Wine produced from date palm (Phoenix dactylifera L.) fruits using Saccharomyces cerevisiae X01 isolated from Nigerian locally fermented beverages.
This study revealed that the Nigerian locally sourced S. cerevisiae X01 can be used as an alternative substrate for industrial scale production of wine with a mild alcoholic content. PMID: 32803346 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - August 16, 2020 Category: Microbiology Authors: Oladoja EO, Oyewole OA, Okeke SK, Azuh VO, Oladoja OI, Jagaba A Tags: Arch Microbiol Source Type: research
Harmful Cyanobacterial Blooms (HCBs): innovative green bioremediation process based on anti-cyanobacteria bioactive natural products.
Abstract Over the last decades, Harmful Cyanobacterial Blooms (HCBs) represent one of the most conspicuous hazards to human health in freshwater ecosystems, due to the uses of the water for drinking, recreation and aquaculture. Cyanobacteria are one of the main biological components in freshwater ecosystems and they may proliferate in nutrients rich ecosystems causing severe impacts at different levels. Therefore, several methods have been applied to control cyanobacterial proliferation, including physical, chemical and biological strategies. However, the application of those methods is generally not very efficien...
Source: Archives of Microbiology - August 14, 2020 Category: Microbiology Authors: Zerrifi SEA, Mugani R, Redouane EM, El Khalloufi F, Campos A, Vasconcelos V, Oudra B Tags: Arch Microbiol Source Type: research
Adhaeribacter soli sp. nov., a bacterium isolated from soil in Korea.
Abstract Strain MA2T was isolated from a soil sample from Gijang-gun, Busan in Korea. The strain, a Gram-stain-negative aerobic bacterium, is non-motile, ovoid- or rod-shaped, catalase- and oxidase-positive, and grows at NaCl concentrations 1% (w/v), at 15-30 °C (optimum 25 °C) and at pH 6-8.5 (optimum pH 7.5). The 16S rRNA gene sequence indicates that it belongs to the genus Adhaeribacter in the family Hymenobacteraceae. Phylogenetically, its closest relatives are Adhaeribacter terrae HY02T and Adhaeribacter terreus DNG6T, to which the strain shows 16S rRNA gene sequence similarity values of 96....
Source: Archives of Microbiology - August 13, 2020 Category: Microbiology Authors: Han JH, Kang S, Kim MK Tags: Arch Microbiol Source Type: research
Maximizing growth and productivity of onion (Allium cepa L.) by Spirulina platensis extract and nitrogen-fixing endophyte Pseudomonas stutzeri.
Abstract The study focuses on the impact of foliar spraying cyanobacterium Spirulina platensis extract and the inoculation with the endophyte N2-fixing Pseudomonas stutzeri, and their mixture in the presence of different nitrogen doses on growth and yield of onion under field conditions. Bioactive compounds of Spirulina and Pseudomonas were analyzed by GC-MC and amino acid production of Spirulina by the amino acid analyzer. Hydrogen cyanide (HCN), indole acetic acid (IAA), ammonia (NH3), pectinase activity, and N2-fixation of Pseudomonas were measured. Plant height (cm), leaf length (cm), number of green leaves, b...
Source: Archives of Microbiology - August 13, 2020 Category: Microbiology Authors: Geries LSM, Elsadany AY Tags: Arch Microbiol Source Type: research
A system to study the expression of phytopathogenic genes encoded by Burkholderia glumae.
Abstract Rice is often infected by bacterial panicle blight disease caused by Burkholderia glumae. Since most studies have assessed the transcriptome of the plant when it is exposed to bacteria, the gene expression of the phytopathogenic bacteria have not been well elaborated during the infection process or in the host cell. Recently, a few researches were conducted to evaluate the in vivo transcriptome of bacteria during the infective process. Most bacterial cells do not express genes involved in pathogenicity in culture medium making it difficult to investigate gene expression of bacterial cells in plant cells. ...
Source: Archives of Microbiology - August 12, 2020 Category: Microbiology Authors: Mirghasempour A, Glick BR, Hou Y, Huang S Tags: Arch Microbiol Source Type: research
Microbial biofilm ecology, in silico study of quorum sensing receptor-ligand interactions and biofilm mediated bioremediation.
Abstract Biofilms are structured microbial communities of single or multiple populations in which microbial cells adhere to a surface and get embedded in extracellular polymeric substances (EPS). This review attempts to explain biofilm architecture, development phases, and forces that drive bacteria to promote biofilm mode of growth. Bacterial chemical communication, also known as Quorum sensing (QS), which involves the production, detection, and response to small molecules called autoinducers, is highlighted. The review also provides a brief outline of interspecies and intraspecies cell-cell communication. Additi...
Source: Archives of Microbiology - August 12, 2020 Category: Microbiology Authors: Balan B, Dhaulaniya AS, Varma DA, Sodhi KK, Kumar M, Tiwari M, Singh DK Tags: Arch Microbiol Source Type: research
Dysbiosis of salivary microbiome and cytokines influence oral squamous cell carcinoma through inflammation.
Abstract Advanced combinatorial treatments of surgery, chemotherapy, and radiotherapy do not have any effect on the enhancement of a 5-year survival rate of oral squamous cell carcinoma (OSCC). The discovery of early diagnostic non-invasive biomarkers is required to improve the survival rate of OSCC patients. Recently, it has been reported that oral microbiome has a significant contribution to the development of OSCC. Oral microbiome induces inflammatory response through the production of cytokines and chemokines that enhances tumor cell proliferation and survival. The study aims to develop saliva-based oral micro...
Source: Archives of Microbiology - August 11, 2020 Category: Microbiology Authors: Rai AK, Panda M, Das AK, Rahman T, Das R, Das K, Sarma A, Kataki AC, Chattopadhyay I Tags: Arch Microbiol Source Type: research
Biodiversity of yeasts isolated during spontaneous fermentation of cool climate grape musts.
Abstract Biodiversity of native yeasts, especially in winemaking, has hidden potential. In order to use the value of non-Saccharomyces strains in wine production and to minimise the possibility of its deterioration, it is necessary to thoroughly study the yeast cultures present on grape fruits and in grape must, as well as their metabolic properties. The aim of the study was to characterise the yeast microbiota found during spontaneous fermentation of grape musts obtained from grape varieties 'Rondo', 'Regent' and 'Johanniter'. Grapes from two vineyards (Srebrna Góra and Zadora) located in southern Poland w...
Source: Archives of Microbiology - August 11, 2020 Category: Microbiology Authors: Cioch-Skoneczny M, Satora P, Skoneczny S, Skotniczny M Tags: Arch Microbiol Source Type: research
Antibiofilm activities of the cinnamon extract against Vibrio parahaemolyticus and Escherichia coli.
This study elucidated that the cinnamon extract was effective to prevent biofilm formation and eradicate mature biofilms of V. parahaemolyticus and E. coli. PMID: 32772125 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - August 9, 2020 Category: Microbiology Authors: Lu C, Liu H, Shangguan W, Chen S, Zhong Q Tags: Arch Microbiol Source Type: research
Unraveling the camel rumen microbiome through metaculturomics approach for agriculture waste hydrolytic potential.
Abstract Cellulose is the most abundant natural polymer present on Earth in the form of agriculture waste. Hydrolysis of agriculture waste for simple fermentable reducing sugars is the bottleneck in the area of biofuel generation and other value-added products. The present study aims to utilize the camel rumen as a bioreactor for potent cellulolytic and hemicellulolytic bacteria by altering the feed types with varying cellulosic concentrations. A total of 6716 bacterial cultures were subjected to three layers of screening, where plate zymography and chromophoric substrate screening served as primary screening ...
Source: Archives of Microbiology - August 8, 2020 Category: Microbiology Authors: Srivastava S, Dafale NA, Jakhesara SJ, Joshi CG, Patil NV, Purohit HJ Tags: Arch Microbiol Source Type: research
Lysobacter gilvus sp. nov., isolated from activated sludge.
Abstract Strain HX-5-24T was isolated from the sludge collected from the outlet of the biochemical treatment facility of an agricultural chemical plant in Maanshan city, Anhui province, PR China (118° 28' N, 31° 47' E). Cells were observed to be Gram-reaction-negative, rod-shaped, non-motile and aerobic. Strain HX-5-24T shared 99.1% 16S rRNA gene sequence similarity with Lysobacter dokdonensis DS-58T and less than 97% similarities with other type strains. The phylogenetic analysis based on 16S rRNA indicated that strain HX-5-24T belonged to the genus Lysobacter and formed a subclade with L. dokdonensis DS-...
Source: Archives of Microbiology - August 7, 2020 Category: Microbiology Authors: Xu J, Sheng M, Yang Z, Qiu J, Zhang J, Zhang L, He J Tags: Arch Microbiol Source Type: research
The genome sequence of the giant phototrophic gammaproteobacterium Thiospirillum jenense gives insight into its physiological properties and phylogenetic relationships.
Abstract In a conserved culture of the purple sulfur bacterium Thiospirillum jenense DSM216T, cells of this species were easily recognized by cell morphology, large-size spirilla and visible flagellar tuft. The Tsp. jenense genome is 3.22 Mb in size and has a GC content of 48.7 mol%. It was readily identified as a member of the Chromatiaceae by the complement of proteins in its genome. A whole genome comparison clearly placed Tsp. jenense near Thiorhodovibrio and Rhabdochromatium species and somewhat more distant from Thiohalocapsa and Halochromatium species. This relationship was also found with the seq...
Source: Archives of Microbiology - August 5, 2020 Category: Microbiology Authors: Imhoff JF, Meyer TE, Kyndt JA Tags: Arch Microbiol Source Type: research
Novel sulphur-oxidizing bacteria consummate sulphur deficiency in oil seed crop.
Abstract Plants absorb sulphate, the oxidized form of elemental sulphur (S°), from soil. Sulphur-oxidizing bacteria play a key role in transformation of sulphur in soil. Oil seed crops require high amount of sulphur and it plays an important role in the formation of proteins, vitamins and enzymes. It increases yield, oil content and protein content in oil seed crops. Sulphur is the important constituent of amino acids, viz. methionine, cystine, and cysteine. It necessitates various enzymatic, metabolic processes such as photosynthesis and nitrogen fixation. In the last few years, the prominence of sulphur in o...
Source: Archives of Microbiology - August 5, 2020 Category: Microbiology Authors: Joshi N, Gothalwal R, Singh M, Dave K Tags: Arch Microbiol Source Type: research
Paenibacillus turpanensis sp. nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China.
Abstract Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach. The optimum growth temperature was 37 °C and the optimum pH was 7.5-8.0. The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids. The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid. The phospholipids were phosphatidylethanolamine, diph...
Source: Archives of Microbiology - August 4, 2020 Category: Microbiology Authors: Yang L, Huang HW, Wang Y, Kou YR, Yin M, Li Y, Wang XQ, Zhao GF, Zhu WY, Tang SK Tags: Arch Microbiol Source Type: research
Pharmacological properties of some mangrove sediment-associated bacillus isolates.
Abstract Mangrove sediment-associated bacteria are of significantly important in the field of medicine and pharmaceuticals as new promising sources of biologically active pharmacophores due to the extreme conditions, such as high salt concentration and soil anoxia. The sediment bacteria associated with Acanthus ilicifolius and Avicennia officinalis collected from the Mangalavanam mangrove ecosystem of the Kerala State of India were evaluated using various in vitro models for the assessment of their pharmacological properties. The bacteria exhibiting significant antioxidant and antimicrobial activities were isolate...
Source: Archives of Microbiology - August 4, 2020 Category: Microbiology Authors: Rajan L, Chakraborty K, Chakraborty RD Tags: Arch Microbiol Source Type: research
Comparative analysis of the gut microbiota composition in the Cln1R151X and Cln2R207X mouse models of Batten disease and in three wild-type mouse strains.
In this study, we analyzed the gut microbiota in Cln1R151X and Cln2R207X mice, models of the childhood neurodegenerative disorders, infantile CLN1 and late infantile CLN2 Batten diseases. Significant alterations were found in the overall gut microbiota composition and also at the individual taxonomic ranks as compared to wild-type mice. The disease-specific alterations in the gut microbiota of Cln1R151X and Cln2R207X mice may contribute to the disease phenotypes observed in these mouse models. We also compared the gut microbiota composition of three wild-type mouse strains frequently used in transgenic studies: 129S6/SvEv,...
Source: Archives of Microbiology - August 4, 2020 Category: Microbiology Authors: Parker C, Zhao J, Pearce DA, Kovács AD Tags: Arch Microbiol Source Type: research
SARS-CoV-2 nucleocapsid and Nsp3 binding: an in silico study.
Abstract Severe acute respiratory syndrome virus 2 (SARS-CoV-2) belongs to the single-stranded positive-sense RNA family. The virus contains a large genome that encodes four structural proteins, small envelope (E), matrix (M), nucleocapsid phosphoprotein (N), spike (S), and 16 nonstructural proteins (nsp1-16) that together, ensure replication of the virus in the host cell. Among these proteins, the interactions of N and Nsp3 are essential that links the viral genome for processing. The N proteins reside at CoV RNA synthesis sites known as the replication-transcription complexes (RTCs). The N-terminal of N has RNA-...
Source: Archives of Microbiology - August 4, 2020 Category: Microbiology Authors: Khan MT, Zeb MT, Ahsan H, Ahmed A, Ali A, Akhtar K, Malik SI, Cui Z, Ali S, Khan AS, Ahmad M, Wei DQ, Irfan M Tags: Arch Microbiol Source Type: research
Polyhydroxyalkanoate synthesis by bacteria isolated from landfill and ETP with pomegranate peels as carbon source.
Abstract Many bacteria produce polyhydroxyalkanoates (PHAs) when exposed to stressful conditions. It is a known fact that PHAs have the potential to replace petrochemical-based plastics as they are biodegradable, biocompatible, and thermoprocessible materials. The study focusses on producing PHA from microbes isolated from polluted environments and pomegranate peels were utilized as a unique carbon source. This was done to ensure reduction in the cost of the substrate that has not yet been reported as a substrate for PHA production. A comparative study was also done with Cupriavidus necator, the reference strain. ...
Source: Archives of Microbiology - August 3, 2020 Category: Microbiology Authors: Rayasam V, Chavan P, Kumar T Tags: Arch Microbiol Source Type: research
Cell-free supernatant of Streptococcus salivarius M18 impairs the pathogenic properties of Pseudomonas aeruginosa and Klebsiella pneumonia.
ccedil;am S Abstract M18 strain of Streptococcus salivarius is a bacterial replacement probiotic that has been suggested for use in the oral cavity. Here, we have shown that S. salivarius M18 cell-free supernatant reduced the growth of the two most common human pathogens Pseudomonas aeruginosa and Klebsiella pneumonia and sensitized the pathogenic bacteria to antibiotic. Besides, the supernatant inhibited biofilm formation of P. aeruginosa drastically. For pinpointing the biomolecular changes that occurred in P. aeruginosa incubated with the probiotic supernatant, attenuated total reflectance-Fourier transform inf...
Source: Archives of Microbiology - August 3, 2020 Category: Microbiology Authors: Tunçer S, Karaçam S Tags: Arch Microbiol Source Type: research
Soil microbial community, enzyme activity, C and N stocks and soil aggregation as affected by land use and soil depth in a tropical climate region of Brazil.
Abstract The impact of agricultural land-use on soil microbial community composition and enzyme activity has not been extensively investigated in Ultisols. We investigated soil health parameters by analyzing phospholipid fatty acids (PLFAs), extracellular enzyme activity, C and N stocks, and soil structure. Four land uses were established in a tropical climate region of Brazil: native Cerrado (savanna), monoculture pasture [Urochloa brizantha (Hochst. Ex A. Rich.) R. Webster 'Marandu'], an integrated crop-livestock system (ICLS), and maize (Zea mays)-fallow in a no-tillage system. Soil microbial biomass was 40% hi...
Source: Archives of Microbiology - August 3, 2020 Category: Microbiology Authors: Sarto MVM, Borges WLB, Bassegio D, Pires CAB, Rice CW, Rosolem CA Tags: Arch Microbiol Source Type: research
Endophytic fungi from Passiflora incarnata: an antioxidant compound source.
In this study, from 315 endophytic fungi isolated from P. incarnata leaves, 60 were selected to determinate presence of chemical constituents related with antioxidant activity, based on their production of soluble pigments. The promising fungi were evaluated specifically on their potential to produce phenolic compounds, flavonoids and for antioxidant activity. Five isolates significantly produced flavonoids and phenolic compounds in the ethyl acetate and n-Butanol extracts, also saponins and high antioxidant activity against the DPPH (2.2-diphenyl-1-picrylhydrazyl) free radical. A strain of Aspergillus nidulans var. dentat...
Source: Archives of Microbiology - August 2, 2020 Category: Microbiology Authors: da Silva MHR, Cueva-Yesquén LG, Júnior SB, Garcia VL, Sartoratto A, de Angelis DF, de Angelis DA Tags: Arch Microbiol Source Type: research
Antimicrobial effect of spore-forming probiotics Bacillus laterosporus and Bacillus megaterium against Listeria monocytogenes.
Abstract There are several reports on the detection of Listeria monocytogenes in Iran, which has been identified as a major public health problem. In addition, due to drug resistance and strong biofilm formation of this pathogen, new approaches such as using probiotics are needed to combat this bacterium. The aim of this study was to investigate the effect of spore-forming probiotics on the expression of virulence genes hly, plc, inlA, and invasion of L. monocytogenes. Spore-forming probiotics were co-cultured with L. monocytogenes at different time points and following cell count, the expression level of virulenc...
Source: Archives of Microbiology - August 2, 2020 Category: Microbiology Authors: Rajabi S, Darban D, Tabatabaei RR, Hosseini F Tags: Arch Microbiol Source Type: research
Non-rhizobial endophytic (NRE) yeasts assist nodulation of Rhizobium in root nodules of blackgram (Vigna mungo L.).
This study focused on unraveling the presence of yeast in the root nodules and their possible function in either nodulation or signal exchange. From the root nodules of blackgram, two yeast strains were isolated and identified as Candida glabrata VYP1 and Candida tropicalis VYW1 based on 18S rRNA gene sequencing and phylogeny. These strains possessed plant growth-promoting traits viz., IAA, ACC deaminase, siderophore, ammonia, and polyamine production. The functional capacity of endophytic yeast strains, and their interaction with Rhizobium sp. was further unveiled via profiling volatile organic compounds (VOC). Among the ...
Source: Archives of Microbiology - July 31, 2020 Category: Microbiology Authors: Geetha Thanuja K, Annadurai B, Thankappan S, Uthandi S Tags: Arch Microbiol Source Type: research
Diclofenac mitigates virulence of multidrug-resistant Staphylococcus aureus.
In conclusion, diclofenac can be used in combination with antibiotics as anti-virulence agent against MDR-MRSA which will enhance the ability of immune system to eradicate infection. PMID: 32737541 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - July 31, 2020 Category: Microbiology Authors: Abbas HA, Atallah H, El-Sayed MA, El-Ganiny AM Tags: Arch Microbiol Source Type: research
Sphingobacterium endophyticum sp. nov., a novel endophyte isolated from halophyte.
Abstract A bacterial strain designated NYYP31T was isolated from the leaves of an annual halophytes, Suaeda corniculata Bunge, collected from the southern edge of the Gurbantunggut desert, north-west China. Strain NYYP31T was Gram-staining negative, strictly aerobic, rod-shaped, non-motile, and non-spore-forming. Growth was observed at 4-42 °C, at pH 5.0-10.0, in the presence of up to 8% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences and coding sequences of 92 protein clusters showed that strain NYYP31T should be assigned to the genus Sphingobacterium. 16S rRNA gene sequence similarity...
Source: Archives of Microbiology - July 31, 2020 Category: Microbiology Authors: Liu YY, Liu F, Li YQ, Lei RF, Ma Q, Narsing Rao MP, Abudourousuli D, Rouzi Z, Aosiman M, An DD, Li WJ Tags: Arch Microbiol Source Type: research
Composition and functional characterization of the gut microbiome of freshwater pufferfish (Tetraodon cutcutia).
This study describes the community composition and functions of the gut microbiome of the freshwater omnivorous pufferfish based on metagenomic approach. Metagenome sequence data showed a dominance of the class Gammaproteobacteria followed by Fusobacteria, Actinobacteria, Anerolineae, Betaproteobacteria, Deinococci, Clostridia and Deltaproteobacteria. At the order level, the most abundant groups were Aeromonadales, Fusobacteriales, Enterobacterales, Synechococcales. The genus Aeromonas was the most predominant followed by Plesiomonas and Cetobacterium. Additionally, within the domain Arch...
Source: Archives of Microbiology - July 31, 2020 Category: Microbiology Authors: Deb S, Das L, Das SK Tags: Arch Microbiol Source Type: research
Identification of bacteria and fungi inhabiting fruiting bodies of Burgundy truffle (Tuber aestivum Vittad.).
Abstract Tuber species may be regarded as complex microhabitats hosting diverse microorganisms inside their fruiting bodies. Here, we investigated the structure of microbial communities inhabiting the gleba of wild growing (in stands) T. aestivum, using Illumina sequencing and culture-based methods. The two methods used in combination allowed to extract more information on complex microbiota of Tuber aestivum gleba. Analysis of the V3-V4 region of 16S rDNA identified nine phyla of bacteria present in the gleba of T. aestivum ascomata, mostly Proteobacteria from the family Bradyrhizobiaceae. Our results ideally mat...
Source: Archives of Microbiology - July 30, 2020 Category: Microbiology Authors: Perlińska-Lenart U, Piłsyk S, Gryz E, Turło J, Hilszczańska D, Kruszewska JS Tags: Arch Microbiol Source Type: research
Evaluation of mtr cluster expression in Shewanella RCRI7 during uranium removal.
In this study, quantitative gene expressions (Real-time RT-PCR) of mtr gene cluster (mtrA, mtrB, mtrC, mtrD, mtrE, mtrF and omcA) in five different uranium concentrations (0.1, 0.25, 0.5, 1 and 2 mM) were performed with ICP and microscopic live cell counting analysis under anaerobic condition, by Shewanella RCRI7 as a native bacterium. The results indicated that the amount of uranium removal and live-cell counting were decreased in the higher uranium concentrations (1 and 2 mM), due to the uranium toxicity, suggesting 0.5 mM as the optimum uranium concentration for Shewanella RCRI7 resistance. The expression...
Source: Archives of Microbiology - July 29, 2020 Category: Microbiology Authors: Ghasemi R, Fatemi F, Mir-Derikvand M, Zarei M Tags: Arch Microbiol Source Type: research
Depression and Toxoplasma gondii infection: assess the possible relationship through a seromolecular case-control study.
In this study, after selecting the patients with depression and control groups under the supervision of a psychologist, the blood samples were collected and the serum samples and buffy coat were separated. The specific anti-Toxoplasma IgG antibodies in serum samples were evaluated using the commercial ELISA kit. Then the desired region of the Toxoplasma B1 gene was amplified using the specific primers. To confirm the specificity of primers to amplify the B1 gene of Toxoplasma, the extracted PCR product was sequenced. The overall prevalence of toxoplasmosis in patients with depression was 59.8 and 60.19% by ELISA and PCR, r...
Source: Archives of Microbiology - July 28, 2020 Category: Microbiology Authors: Nasirpour S, Kheirandish F, Fallahi S Tags: Arch Microbiol Source Type: research
Metagenomic profiling of bacterial diversity and community structure in termite mounds and surrounding soils.
This study also revealed a lot of unclassified groups of bacteria and this could point to the presence of potentially novel species. The differences observed in the bacterial structure and diversity from this study may be ascribed to variances in the physicochemical nature existing between the two environments. Mapping out schemes to culture these unclassified groups of bacteria discovered from this study would possibly set the platform for the discovery of novel bacteria for biotechnological applications. PMID: 32725600 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - July 28, 2020 Category: Microbiology Authors: Enagbonma BJ, Ajilogba CF, Babalola OO Tags: Arch Microbiol Source Type: research
Plant-bacterial endophyte secondary metabolite matching: a case study.
Abstract Some studies focused on metabolic relationships between plants and their endophytic bacterial associates, and more research is required to generate critical evidence for these relationships. In the current interest, we tried to confirm the relationship between the traditional medicinal plant, Calotropis procera (Aiton) W.T. Aiton, and its associated endophytes, Bacillus siamensis and Bacillus amyloliquefaciens, as the first matching study regarding the production of bioactive secondary metabolites from the plant vis-a-vis its bacterial endophytes.Secondary metabolites of both the plant and its endophytic ...
Source: Archives of Microbiology - July 28, 2020 Category: Microbiology Authors: Hagaggi NSA, Mohamed AAA Tags: Arch Microbiol Source Type: research
Characterization and expression pattern analysis of pheromone receptor-like genes in Winter Mushroom Flammulina filiformis.
This study suggests that PRLGs could be vital genes involving in fruiting body development in F. filiformis. However, further studies could be performed to reveal their specific functional pathways in the fruiting body development. PMID: 32719947 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - July 27, 2020 Category: Microbiology Authors: Meng L, Chou T, Jiang S, Wang L, Zhu M, Mukhtar I, Xie B, Wang W Tags: Arch Microbiol Source Type: research
Flaviaesturariibacter flavus sp. nov., isolated from soil in Jeju Island.
Abstract A Gram-stain-negative, non-motile and yellow-colored bacterium, designated 17J68-12T, was isolated from soil in Jeju Island, Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 17J68-12T formed a distinct lineage within the family Chitinophagaceae and was mostly related to members of Flaviaesturariibacter luteus (97.5%), Flaviaesturariibacter amylovorans (96.8%) and Flaviaesturariibacter terrae (96.8%). Growth was observed at 18-42 °C (optimum 30 °C) in R2A broth at pH 7.0. The major cellular fatty acids of the strain 17J68-12 T were summed feature 3 (...
Source: Archives of Microbiology - July 27, 2020 Category: Microbiology Authors: Jang JH, Maeng SH, Jung HY, Kim MK, Subramani G Tags: Arch Microbiol Source Type: research