Biocharacteristics and draft genome sequence of Halomonas hydrothermalis C22, a pyruvate-producing halophilic bacterium isolated from a commercial Spirulina culture in Vietnam.
Abstract Halophilic bacteria are receiving increasing attention for industrial chemical production processes due to their unique properties. Herein, an alkaliphilic and halophilic bacterium was isolated from a commercial Spirulina culture at Nghe An province in Vietnam and found to secrete pyruvate. Pyruvate is widely used as a starting material in the industrial biosynthesis of pharmaceuticals, and is employed for production of crop protection agents, polymers, cosmetics, and food additives. Phenotypic and chemotaxonomic characterization, and the 16S rRNA gene sequence homology with Halomonas hydrothermalis strai...
Source: Archives of Microbiology - January 23, 2020 Category: Microbiology Authors: Anh HTL, Kawata Y, Tam LT, Thom LT, Ha NC, Hien HTM, Thu NTH, Huy PQ, Hong DD Tags: Arch Microbiol Source Type: research

Anoxic cell rupture of Prevotella bryantii by high-pressure homogenization protects the Na+-translocating NADH:quinone oxidoreductase from oxidative damage.
Abstract Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na+-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQR activity and protein yield after oxic and anoxic cell disruption by the EmulsiFlex, by ultrasonication, and by glass beads treatment. With an overall membrane protein yield of 50 mg L-1 culture and a NADH oxidation activity of 0.8 µmol min-1 mg-1, the EmulsiFlex was the most effic...
Source: Archives of Microbiology - January 18, 2020 Category: Microbiology Authors: Schleicher L, Fritz G, Seifert J, Steuber J Tags: Arch Microbiol Source Type: research

Vesicular transport and secretion of penicillin G in Penicillium rubens P2-32-T.
rnández FJ Abstract The compartmentalization of penicillin G biosynthesis in Penicillium rubens has been extensively studied. However, how this compound is secreted has not been completely elucidated, although its transport could be of the vesicular type. This work was aimed at observing vesicles and penicillin secretion and proposing a hypothetical model for their compartmentalization and secretion. For this purpose, a high-penicillin-producing strain (P. rubens P2-32-T) was compared by transmission electron microscopy (TEM) and atomic force microscopy (AFM) with a null-producing strain (P. rubens npe10) i...
Source: Archives of Microbiology - January 17, 2020 Category: Microbiology Authors: Campos C, Lázaro-Rodríguez TG, Fragoso-Soriano R, Fernández FJ Tags: Arch Microbiol Source Type: research

Description of Gemmobacter aestuarii sp. nov., isolated from estuarine surface water and reclassification of Cereibacter changlensis as Gemmobacter changlensis Chen et al. 2013.
Abstract A Gram-stain-negative, tyrosine-metabolizing, non-motile, strictly aerobic, non-spore-forming, rod-shaped marine bacterium, designated strain CC-PW-75T, was isolated from the estuarine water off Pintung, Taiwan. Strain CC-PW-75T formed a distinct phyletic lineage associated with Gemmobacter species, sharing the highest 16S rRNA gene sequence similarity with G. megaterium CF17T and G. straminiformis CAM-8T (96.0% each) followed by G. aquatilis IFAM 1031T and G. nectariphilus AST4T (95.8% each). Analysis of the draft genome (3.76 Mbp) revealed the presence of genes encoding light-harvesting complexes, ...
Source: Archives of Microbiology - January 16, 2020 Category: Microbiology Authors: Hameed A, Shahina M, Lin SY, Chen WM, Hsu YH, Lai WA, Young CC Tags: Arch Microbiol Source Type: research

Immunomagnetic separation-based nanogold enhanced surface plasmon resonance and colloidal gold test strips for rapid detection of Vibrio parahaemolyticus.
Abstract Nanogold enhanced surface plasmon resonance (SPR), colloidal gold immunochromatographic test strips (ICTS), and polymerase chain reaction (PCR), combined with immunomagnetic separation (IMS) were established in this study for the rapid detection of Vibrio parahaemolyticus (VP). The sensitivities of SPR, ICTS, and PCR was determined to be 101, 103, and 103 CFU/mL for VP, respectively. After separation and enrichment by IMS, the sensitivities of SPR, ICTS, and PCR were 100, 101, and 102 CFU/mL for VP, respectively, which were improved by 10-, 100-, and 10-fold compared to the direct detection by S...
Source: Archives of Microbiology - January 14, 2020 Category: Microbiology Authors: Zhou J, Zhang C, Zhang X, Lu C, Ming T, Li Y, Su X Tags: Arch Microbiol Source Type: research

Archives of microbiology: screening of pectinase-producing bacteria from citrus peel and characterization of a recombinant pectate lyase with applied potential.
Abstract Pectinase is widely used in numerous industrial fields, including the food, wine, and paper industries. In this work, seven bacteria were isolated from orange peel and their pectinase production activity was assayed. One bacterium (OR-B2) identified as a Bacillus sp. showed the highest enzyme activity towards others. A gene encoding a pectate lyase designed as PelB-B2 in this work was amplified and heterogeneous expressed in E.coli. PelB-B2 was defined as a member of the PelB pectate lyase family after phylogenic tree analysis. 3D model of PelB-B2 was constructed by SWISS-MODEL and PelB-B2 showed conserve...
Source: Archives of Microbiology - January 13, 2020 Category: Microbiology Authors: Guan Y, Wang D, Lv C, Zhang Y, Gelbic I, Ye X Tags: Arch Microbiol Source Type: research

Inoculation of plant growth-promoting bacteria attenuates the negative effects of drought on sorghum.
ute;nior PI Abstract Microbial inoculants are suitable cost-effective technology to help plants endure drought. For the development of commercial inoculants, screening of efficient plant growth-promoting bacteria (PGPB) is a crucial step. The aim of this study was to evaluate the performance of PGPB to modulate drought resistance in Sorghum bicolor. A pot experiment with sorghum was conducted to access the role of previously selected PGPB strains. In addition, two non-inoculated control treatments (with and without urea fertilization) were also evaluated. For comparison, a fully irrigated treatment (FIT) was also ...
Source: Archives of Microbiology - January 13, 2020 Category: Microbiology Authors: Santana SRA, Voltolini TV, Antunes GDR, da Silva VM, Simões WL, Morgante CV, de Freitas ADS, Chaves ARM, Aidar ST, Fernandes-Júnior PI Tags: Arch Microbiol Source Type: research

Current approaches and trends in the production of microbial cellulases using residual lignocellulosic biomass: a bibliometric analysis of the last 10  years.
Current approaches and trends in the production of microbial cellulases using residual lignocellulosic biomass: a bibliometric analysis of the last 10 years. Arch Microbiol. 2020 Jan 11;: Authors: Roth JCG, Hoeltz M, Benitez LB Abstract Considering the potential use of lignocellulosic biomass residues in microbial cultures to produce cellulases, the objective of this research was to investigate trends and discussions regarding scientific research conducted in this field through a bibliometric and scientometric analysis. Using the Elsevier Scopus database and VOSviewer software, scientific papers ...
Source: Archives of Microbiology - January 11, 2020 Category: Microbiology Authors: Roth JCG, Hoeltz M, Benitez LB Tags: Arch Microbiol Source Type: research

Metal ions and graphene-based compounds as alternative treatment options for burn wounds infected by antibiotic-resistant Pseudomonas aeruginosa.
This study aimed to determine the antimicrobial effect of metal ions, graphene (Gr), and graphene oxide (GO), individually and in combination, against the planktonic and biofilm states of two antimicrobially resistant clinical strains of P. aeruginosa each with different antibiotic resistance profiles. Minimum inhibitory, minimum bactericidal, and fractional inhibitory concentrations were performed to determine the efficacy of the metal ions and graphene composites individually and their synergy in combination. Crystal violet biofilm and XTT assays measured the biofilm inhibition and metabolic activity, respectively. Molyb...
Source: Archives of Microbiology - January 8, 2020 Category: Microbiology Authors: Karaky N, Kirby A, McBain AJ, Butler JA, El Mohtadi M, Banks CE, Whitehead KA Tags: Arch Microbiol Source Type: research

Characterization of the gut microbiome of black-necked cranes (Grus nigricollis) in six wintering areas in China.
Abstract The black-necked crane (Grus nigricollis) is a vulnerable species, breeding exclusively on the high-altitude wetlands of the Qinghai-Tibet Plateau. Bird species harbor diverse communities of microorganisms within their gastrointestinal tracts, which have important roles in the health, nutrition, and physiology of birds. Hitherto, virtually nothing was known about the gut microbial communities associated with wild black-necked cranes. For the first time, this study characterized the gut microbial community compositions, diversity, and functions of black-necked cranes from six wintering areas in China using...
Source: Archives of Microbiology - January 4, 2020 Category: Microbiology Authors: Wang W, Wang F, Li L, Wang A, Sharshov K, Druzyaka A, Lancuo Z, Wang S, Shi Y Tags: Arch Microbiol Source Type: research

Marine macroalgae-associated heterotrophic Firmicutes and Gamma-proteobacteria: prospective anti-infective agents against multidrug resistant pathogens.
Abstract The development of drug-resistant bacteria and the necessity for unique antimicrobial agents, directed to the search of new habitats to screen the production of anti-infective substances. Culture-dependent studies of heterotrophic bacteria from the intertidal macroalgae thriving along the Southern coast of India resulted in the isolation of 148 strains, which were assayed for antibacterial activities against wide spectrum of pathogens including drug-resistant pathogens, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VRE). Two of the most active strains w...
Source: Archives of Microbiology - January 2, 2020 Category: Microbiology Authors: Kizhakkekalam VK, Chakraborty K Tags: Arch Microbiol Source Type: research

Alleviation of tetrabromobisphenol A toxicity in soybean seedlings by Rhodopseudomonas palustris RP1n1.
This study investigated the regulatory role of Rhodopseudomonas palustris RP11 in alleviating TBBPA-induced harmful effects in soybean seedlings. In this study, the characteristics of growth promotion by strain RP11 were studied by analysing 5-aminolevulinic acid (ALA) and indole-3-acetic acid (IAA) production, as well as phosphorus-solubilizing and potassium-solubilizing ability. In the pot culture conditions, we tested whether strain RP11 improved soybean seedlings tolerance against TBBPA by measuring the root length and physiological parameters of the seedlings treated with strain RP11 and different concentration of TBB...
Source: Archives of Microbiology - January 2, 2020 Category: Microbiology Authors: Ge H, Liu Z Tags: Arch Microbiol Source Type: research

Microbial inoculants: potential tool for sustainability of agricultural production systems.
Abstract Microbial inoculants are gaining importance for attaining sustainable agricultural production systems. Nutrient supply capacity of soil is diminishing continuously owing to soil erosions, degradation, deposition of salts, undesirable elements and metals, water scarcity or excess and imbalanced nutrient supply system. Numerous complementary microbial inoculation combinations are contributing immensely in the management of plant nutrients by way of fixation, solubilization or transformation in soil. Thus, biological wastes and microbial inoculants are alternatives for nutrient demands to bridge future gaps ...
Source: Archives of Microbiology - January 2, 2020 Category: Microbiology Authors: Sammauria R, Kumawat S, Kumawat P, Singh J, Jatwa TK Tags: Arch Microbiol Source Type: research

Amelioration effect of chromium-tolerant bacteria on growth, physiological properties and chromium mobilization in chickpea (Cicer arietinum) under chromium stress.
In this study, chromium (Cr)-tolerant bacteria were test for their efficiency in alleviating Cr stress in Cicer arietinum plants. On the basis of 16S rRNA gene analysis, the isolates were identified belonging to genus Stenotrophomonas maltophilia, Bacillus thuringiensis B. cereus, and B. subtilis. The strains produced a considerable amount of indole-3-acetic acid in a medium supplemented with tryptophan. The strains also showed siderophore production (S2VWR5 and S3VKR17), phosphorus production (S1VKR11, S3VKR2, S3VKR16, and S2VWR5), and potassium solubilization (S3VKR2, S2VWR5, and S3VKR17). Furthermore, the strains were e...
Source: Archives of Microbiology - January 1, 2020 Category: Microbiology Authors: Shreya D, Jinal HN, Kartik VP, Amaresan N Tags: Arch Microbiol Source Type: research

Composition and functional diversity of fecal bacterial community of wild boar, commercial pig and domestic native pig as revealed by 16S rRNA gene sequencing.
This study aimed to investigate the fecal bacterial diversity of wild boar and compare with commercial pig and domestic native pig. The diet composition showed that the diets of wild boar, commercial pig and domestic native pig were different from each other. More than 1,760,000 quality-filtered sequences were obtained, and the results revealed distinct compositions and diversity of fecal microbiota in three groups. PCoA and NMDS analyses showed that fecal bacterial communities of wild boar, commercial pig and domestic native pig formed distinctly different clusters. Although the three groups shared a large size of OTUs co...
Source: Archives of Microbiology - January 1, 2020 Category: Microbiology Authors: Huang J, Zhang W, Fan R, Liu Z, Huang T, Li J, Du T, Xiong T Tags: Arch Microbiol Source Type: research

Influence of different culture conditions on exopolysaccharide production by indigenous lactic acid bacteria isolated from pickles.
The objective of this study was to assess the effects of some culture conditions [temperature (20, 30, 37 °C), incubation time (48, 72, 120 h), pH (5.0, 6.0, 7.0), NaCl concentration (0, 3, 6%), carbon (glucose, fructose, lactose), nitrogen (sodium nitrate, ammonium sulfate, bacto-peptone), and mineral sources (calcium carbonate, ferric chloride)] on the exopolysaccharide (EPS) production by lactic acid bacteria (LAB) strains (belonging to Lactobacillus (L.) plantarum, L. namurensis, and Pediococcus (P.) ethanolidurans species) isolated from naturally fermented pickles. The maximum EPS production was determin...
Source: Archives of Microbiology - January 1, 2020 Category: Microbiology Authors: Mıdık F, Tokatlı M, Bağder Elmacı S, Özçelik F Tags: Arch Microbiol Source Type: research

Illumina MiSeq sequencing and network analysis the distribution and co-occurrence of bacterioplankton in Danjiangkou Reservoir, China.
This study investigated the bacterioplankton community composition in the surface water of the Danjiangkou Reservoir using the Illumina MiSeq sequencing platform. We observed that the bacterioplankton community primarily consisted of 27 phyla and 336 genera, including Actinobacteria, Proteobacteria, and Bacteroidetes, demonstrating the richness of the community composition. Redundancy analysis of the bacterioplankton communities and environmental variables showed that the total nitrogen (TN), pH, chemical oxygen demand (COD), and permanganate index (CODMn) were important factors affecting the bacterioplankton distribution....
Source: Archives of Microbiology - January 1, 2020 Category: Microbiology Authors: Chen ZJ, Xu G, Ding CY, Zheng BH, Chen Y, Han H, Li YY, Shi JW, Hu LQ Tags: Arch Microbiol Source Type: research

Fish borne Edwardsiella tardaeha involved in the bacterial biofilm formation, hemolytic activity, adhesion capability and pathogenicity.
Abstract Edwardsiella tarda (E. tarda) is distributed widely in a variety of hosts including humans, other mammals and fish, and it is worthwhile to notice that E. tarda -caused fish infections lead to the most important bacterial disease in fish. Considering Eha acting as a transcriptional regulator in E. tarda strain ET13 have been reported previously, to better understand its pathogenesis due to this, a type of cell of epithelial cell line (Caco-2) infection model for the pathogen was established in the laboratory. We focused on studying various parameters such as lactate dehydrogenase release (to measure cytot...
Source: Archives of Microbiology - December 21, 2019 Category: Microbiology Authors: Hassan HA, Ding X, Zhang X, Zhu G Tags: Arch Microbiol Source Type: research

Dissecting microbial community structure in sewage treatment plant for pathogens' detection using metagenomic sequencing technology.
Abstract Continuous observation of wastewater treatment plants is very crucial to keep them safe for proper use and protection from pathogenic contamination. Illumina sequencing technology was used for microbiome structuring from various samples taken from different portions of the wastewater treatment plant, including influent, activated, return sludge and effluent, where different microbial compositions were found. The effluent section was found to have pathogenic microbes such as viruses, Alpha- and deltaproteobacteria, Actinobacteria, Bacteroidetes, clostridia, and bacilli in various concentrations. The presen...
Source: Archives of Microbiology - December 17, 2019 Category: Microbiology Authors: Giwa AS, Ali N, Athar MA, Wang K Tags: Arch Microbiol Source Type: research

Identification and genetic analysis of a totivirus isolated from the Culex tritaeniorhynchus in northern China.
Abstract Totiviridae, a viral family of double-stranded RNA (dsRNA) viruses, contain a single dsRNA genome 4.6-7.0 kb in length. Totiviridae were initially only known to infect fungi and other eukaryotes as well as plants, but an increase in totiviruses has been detected in insects, mosquitoes, and bats. Here, we describe the isolation and characterization of a strain belonging to the family Totiviridae isolated from Culex tritaeniorhynchus in Kenli, China, in 2016. We isolated a totivirus from field-collected mosquitoes in China by cell culture in Aedes albopictus C6/36 cells, identified the virus by morphol...
Source: Archives of Microbiology - December 16, 2019 Category: Microbiology Authors: Li F, Du J, Wu Z, Zhang W, Fu S, Song J, Wang Q, He Y, Lei W, Xu S, Xu A, Zhao L, Liang G, Wang H Tags: Arch Microbiol Source Type: research

Genome analysis of the marine bacterium Kiloniella laminariae and first insights into comparative genomics with related Kiloniella species.
We report on the analysis of the draft genome of this alphaproteobacterium and describe some selected features. The 4.4 Mb genome has a G + C content of 51.4%, contains 4213 coding sequences including 51 RNA genes as well as 4162 protein-coding genes, and is a part of the Genomic Encyclopaedia of Bacteria and Archaea (GEBA) project. The genome provides insights into a number of metabolic properties, such as carbon and sulfur metabolism, and indicates the potential for denitrification and the biosynthesis of secondary metabolites. Comparative genome analysis was performed with K. laminariae LD81T and the ...
Source: Archives of Microbiology - December 16, 2019 Category: Microbiology Authors: Wiese J, Imhoff JF, Horn H, Borchert E, Kyrpides NC, Göker M, Klenk HP, Woyke T, Hentschel U Tags: Arch Microbiol Source Type: research

Efficacy of voriconazole in vitro and in invertebrate model of cryptococcosis.
Abstract Cryptococcosis is a common opportunistic infection in patients with advanced HIV infection and may also affect immunocompetent patients. The available antifungal agents are few and other options are needed for the cryptococcosis treatment. In this work, we first analyzed the virulence of twelve C. neoformans and C. gattii strains assessing capsule thickness, biofilms formation, and survival and morbidity in the invertebrate model of Galleria mellonella and then we evaluated the antifungal activity of voriconazole (VRC) in vitro and in vivo also using G. mellonella. Our results showed that all Cryptococcus...
Source: Archives of Microbiology - December 12, 2019 Category: Microbiology Authors: de Castro Spadari C, da Silva de Bastiani FWM, Pisani PBB, de Azevedo Melo AS, Ishida K Tags: Arch Microbiol Source Type: research

Alterations of salivary microbial community associated with oropharyngeal and hypopharyngeal squamous cell carcinoma patients.
Abstract The highest number (35.1% of global incident cases) of new oropharyngeal (OP) and hypopharyngeal (HP) cancer cases was reported in South-Central Asia. The highest incidence of HP cancer in India was reported in East Khasi Hills District of Meghalaya, Aizawl District of Mizoram, and Kamrup Urban District of Assam. HP and OP cancer showed the highest mortality rate, worst prognoses and the highest rate of nodal metastases and distant metastases. Thus, research is required to detect specific biomarkers for early prevention and diagnosis for these cancers. Oral microbiome signatures in saliva are considered a...
Source: Archives of Microbiology - December 12, 2019 Category: Microbiology Authors: Panda M, Rai AK, Rahman T, Das A, Das R, Sarma A, Kataki AC, Chattopadhyay I Tags: Arch Microbiol Source Type: research

Acholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor).
We describe two novel species of Acholeplasma sp. strain N93 and Mycoplasma sp. strain LR5794 which were isolated from the nasopharynx of a horse from the United Kingdom and from the oral cavity of a North American raccoon from Canada, respectively. These strains were phenotypically and genetically characterized and compared to other established Mycoplasma and Acholeplasma species. Both strains are facultative anaerobes, resistant to penicillin, and produce acid from glucose but do not hydrolyze arginine and urea. Both strains grew well in microaerophilic and anaerobic atmospheric conditions at 35-37 °C using PPLO...
Source: Archives of Microbiology - December 11, 2019 Category: Microbiology Authors: Volokhov DV, Gao Y, Davidson MK, Chizhikov VE Tags: Arch Microbiol Source Type: research

Comparative analysis of Enterococcus faecalis and Enterococcus faecium strains isolated from clinical samples and traditional cheese types in the Northwest of Iran: antimicrobial susceptibility and virulence traits.
Abstract This research was conducted using 50 samples of popular traditional cheeses and 160 enterococcal clinical isolates. Phenotypic and genotypic methods used for identification of enterococci. Then, the incidences of antibacterial resistance and virulence traits were investigated. In total, 165 E. faecalis and 43 E. faecium obtained from traditional cheeses and different clinical isolates were analyzed in the study. Antibiotic susceptibility testing revealed 175(84.1%) isolates with multi-drug resistance (MDR) patterns, which was more common among clinical sources. The predominant virulence profile, including...
Source: Archives of Microbiology - December 10, 2019 Category: Microbiology Authors: Jahansepas A, Sharifi Y, Aghazadeh M, Ahangarzadeh Rezaee M Tags: Arch Microbiol Source Type: research

Dominant mutants of the calcineurin catalytic subunit (CNA-1) showed developmental defects, increased sensitivity to stress conditions, and CNA-1 interacts with CaM and CRZ-1 in Neurospora crassa.
Abstract We studied a dominant mutant of the Neurospora crassa calcineurin catalytic (cna-1) subunit generated using the repeat-induced point mutation (RIP). The Cna-1RIP mutants showed defects in morphology, aerial hyphae development, carotenoid accumulation, and fertility. The Cna-1RIP mutants also showed sensitivity to osmotic stress and a reduction in acquisition of thermotolerance on exposure to lethal heat shock temperature. The Cna-1RIP allele was dominant over the wild type cna-1 allele, suggesting that the CNA-1RIP mutant protein acts in a dominant-negative manner. In addition, we studied calcineurin regu...
Source: Archives of Microbiology - December 5, 2019 Category: Microbiology Authors: Kumar A, Roy A, Deshmukh MV, Tamuli R Tags: Arch Microbiol Source Type: research

Identification and partial purification of thuricin 4AJ1 produced by Bacillus thuringiensis.
Abstract Thuricin 4AJ1, produced by Bacillus thuringiensis strain 4AJ1, showed inhibition activity against Bacillus cereus 0938 and ATCC 10987. It began to appear in the stationary phase and reached its maximum activity level of 209.958 U at 18 h against B. cereus 0938 and 285.689 U at 24 h against B. cereus ATCC 10987. Tricine-SDS-PAGE results showed that the partly purified thuricin 4AJ1 was about 6.5 kDa. The molecular weights of the known B. thuringiensis bacteriocins and the ones obtained by the two mainstream websites for predicting bacteriocins were inconsistent with the size of the thuricin ...
Source: Archives of Microbiology - December 5, 2019 Category: Microbiology Authors: Su X, Li L, Pan J, Fan X, Ma S, Guo Y, Idris AL, Zhang L, Pan X, Gelbič I, Huang T, Guan X Tags: Arch Microbiol Source Type: research

Methylobacterium segetis sp. nov., a novel member of the family Methylobacteriaceae isolated from soil on Jeju Island.
Abstract A bacterial strain, 17J42-1T, was isolated from a soil sample collected on Jeju Island, Republic of Korea. Colonies grown on R2A agar were pink in color, and cells were Gram-stain negative, short and rod-shaped. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Methylobacterium in the family Methylobacteriaceae, with high levels of 16S rRNA sequence similarity shared with Methylobacterium oxalidis 35aT (98.6%), Methylobacterium jeotgali S2R03-9T (97.5%), and Methylobacterium soli YIM 48816T (97.3%). Cells grew at 15-35 °C, pH 5-9, and in the presence of 0-1.0% Na...
Source: Archives of Microbiology - December 3, 2019 Category: Microbiology Authors: Ten LN, Li W, Elderiny NS, Kim MK, Lee SY, Rooney AP, Jung HY Tags: Arch Microbiol Source Type: research

Glutamicibacter mishrai sp. nov., isolated from the coral Favia veroni from Andaman Sea.
Abstract A novel aerobic marine actinobacterium (strain S5-52T) belonging to the genus Glutamicibacter was isolated from the coral Favia veroni sampled from the Andaman Sea, India. Cells are Gram stain positive and rod shaped. The DNA G+C content was 58.7 mol%. The major quinones were MK-8 and MK-9. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid, trimannosyldiacylglycerol, phospholipid and dimannosylglyceride. The peptidoglycan type was A4α. Strain S5-52T showed a maximum 16S rRNA similarity of 99.36% with Glutamicibacter halophytocola DSM 101718T. The genome of strain S5...
Source: Archives of Microbiology - December 3, 2019 Category: Microbiology Authors: Das L, Deb S, Das SK Tags: Arch Microbiol Source Type: research

Acinetobacter pullicarnis sp. nov. isolated from chicken meat.
Abstract A novel bacterial strain, named S23T, was isolated from chicken meat of local market in Korea. Cells were Gram-negative, milky-yellow colored, non-motile and coccobacillus. The strain was obligate aerobic and catalase-positive, oxidase-negative, optimum growth temperature and pH were 25 °C and pH 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain S23T belongs to the genus Acinetobacter and is most closely related to Acinetobacter defluvii KCTC 52503 T (97.40%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) value between strain S23T and...
Source: Archives of Microbiology - December 2, 2019 Category: Microbiology Authors: Han RH, Lee JE, Yoon SH, Kim GB Tags: Arch Microbiol Source Type: research

Inhibition of ergosterol synthesis in Candida albicans by novel eugenol tosylate congeners targeting sterol 14 α-demethylase (CYP51) enzyme.
This study is a continuation and extension of our previous study in which we synthesized seven novel eugenol tosylate congeners (ETC-1 to ETC-7) from a natural compound eugenol and checked their antifungal activity against different isolates of Candida albicans. All these ETCs showed potent antifungal activity to varying degrees. In this study, the aim is to evaluate the effect of most active compounds (ETC-5, ETC-6 and ETC-7) on ergosterol biosynthesis pathway and cellular viability in C. albicans by applying combined approach of in silico and in vitro methodologies. In silico studies were done through all atom molecular ...
Source: Archives of Microbiology - November 30, 2019 Category: Microbiology Authors: Lone SA, Khan S, Ahmad A Tags: Arch Microbiol Source Type: research

Characterization and comparison of CRISPR Loci in Streptococcus thermophilus.
Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) consists of a series of regular repeat-spacer sequences. It can not only act as a natural immune system in most prokaryotes, but also be utilized as the tool of newly developed genome modification and evolutionary researches. Streptococcus thermophilus is an important model organism for the study and application of CRISPR systems. In present study, the occurrence and diversity of CRISPR-Cas systems in the genomes of S. thermophilus were investigated including 4 new sequenced strains CS5, CS9, CS18, CS20, and other 23 strains downloaded fro...
Source: Archives of Microbiology - November 28, 2019 Category: Microbiology Authors: Hu T, Cui Y, Qu X Tags: Arch Microbiol Source Type: research

Revisiting the plant growth-promoting rhizobacteria: lessons from the past and objectives for the future.
This report provides comprehensive reviews and discusses beneficial effects of PBR on plant and soil health. Considering their multitude of functions to improve plant and soil health, we propose to call the plant growth-promoting bacteria (PGPR) as PBR. PMID: 31781809 [PubMed - as supplied by publisher] (Source: Archives of Microbiology)
Source: Archives of Microbiology - November 28, 2019 Category: Microbiology Authors: Aeron A, Khare E, Jha CK, Meena VS, Aziz SMA, Islam MT, Kim K, Meena SK, Pattanayak A, Rajashekara H, Dubey RC, Maurya BR, Maheshwari DK, Saraf M, Choudhary M, Verma R, Meena HN, Subbanna ARNS, Parihar M, Shukla S, Muthusamy G, Bana RS, Bajpai VK, Han YK, Tags: Arch Microbiol Source Type: research

Bradyrhizobium sp. enhance ureide metabolism increasing peanuts yield.
This study aimed to evaluate the effects of seed inoculation with Bradyrhizobium sp. and co-inoculation with Azospirillum brasilense. The seed treatments were as follows: control (without inoculation); A. brasilense (2 mL per kg-1 of seed); A. brasilense (4 mL per kg-1 of seed); Bradyrhizobium sp. (2 mL per kg-1 of seed); Bradyrhizobium sp. (4 mL per kg-1 of seed); A. brasilense + Bradyrhizobium sp. (2 mL of each strain per kg-1 of seed); and A. brasilense + Bradyrhizobium sp. (4 mL of each strain per kg-1 of seed). Peanut plants from seeds inoculated with Bradyrhiz...
Source: Archives of Microbiology - November 27, 2019 Category: Microbiology Authors: Gericó TG, Tavanti RFR, de Oliveira SC, Lourenzani AEBS, de Lima JP, Ribeiro RP, Dos Santos LCC, Dos Reis AR Tags: Arch Microbiol Source Type: research

Lysobacter terrigena sp. nov., isolated from a Korean soil sample.
Abstract A bacterial strain isolated from a soil collected in Jeju Island, designated as 17J7-1T, was Gram-negative, rod-shaped, yellow colored, and motile by gliding. This strain was able to grow at temperature range from 10 to 42 °C, pH 7-9, and tolerated up to 1% NaCl. Analysis of 16S rRNA sequence identified strain 17J7-1T as a member of the genus Lysobacter with close sequence similarity with Lysobacter mobilis 9NM-14T (97.4%), Lysobacter xinjiangensis RCML-52T (97.0%), and Lysobacter humi FJY8T (96.9%). The genomic DNA G + C content of the isolate was 67.9 mol%. DNA-DNA relatednes...
Source: Archives of Microbiology - November 27, 2019 Category: Microbiology Authors: Li W, Elderiny NS, Ten LN, Lee SY, Kim MK, Jung HY Tags: Arch Microbiol Source Type: research

Inhibition of biofilm formation of Pseudomonas aeruginosa by caffeine: a potential approach for sustainable management of biofilm.
Abstract Pseudomonas aeruginosa is a potent biofilm forming organism causing several diseases on host involving biofilm. Several natural and synthetic molecules have been explored towards inhibiting the biofilm formation of Pseudomonas aeruginosa. In the current report, the role of a natural molecule namely caffeine was examined against the biofilm forming ability of P. aeruginosa. We have observed that caffeine shows substantial antimicrobial activity against P. aeruginosa wherein the minimum inhibitory concentration (MIC) of caffeine was found to be 200 μg/mL. The antibiofilm activity of caffeine was det...
Source: Archives of Microbiology - November 27, 2019 Category: Microbiology Authors: Chakraborty P, Dastidar DG, Paul P, Dutta S, Basu D, Sharma SR, Basu S, Sarker RK, Sen A, Sarkar A, Tribedi P Tags: Arch Microbiol Source Type: research

Bacillus subtilis, an ideal probiotic bacterium to shrimp and fish aquaculture that increase feed digestibility, prevent microbial diseases, and avoid water pollution.
Abstract Beneficial microorganisms maintain the ecosystems, plants, animals and humans working in healthy conditions. In nature, around 95% of all microorganisms produce beneficial effects by increasing nutrients digestion and assimilation, preventing pathogens development and by improving environmental parameters. However, increase in human population and indiscriminate uses of antibiotics have been exerting a great pressure on agriculture, livestock, aquaculture, and also to the environment. This pressure has induced the decomposition of environmental parameters and the development of pathogenic strains res...
Source: Archives of Microbiology - November 26, 2019 Category: Microbiology Authors: Olmos J, Acosta M, Mendoza G, Pitones V Tags: Arch Microbiol Source Type: research

Characterization of gallium resistance induced in a Pseudomonas aeruginosa cystic fibrosis isolate.
Maeda T, García-Contreras R Abstract The repurposing of gallium nitrate as an antibacterial, a drug used previously for the treatment of hypercalcemia, is a plausible alternative to combat infections by Pseudomonas aeruginosa, since it has antipseudomonal properties in vitro and in vivo in animal models and in human lung infections. Furthermore, gallium nitrate tolerance in clinical isolates is very rare. Nevertheless, studies on the reference strains PA14 and PAO1 show that resistance against gallium nitrate is achieved by decreasing gallium intracellular levels by increasing the production of pyocyanin. ...
Source: Archives of Microbiology - November 26, 2019 Category: Microbiology Authors: Tovar-García A, Angarita-Zapata V, Cazares A, Jasso-Chávez R, Belmont-Díaz J, Sanchez-Torres V, López-Jacome LE, Coria-Jiménez R, Maeda T, García-Contreras R Tags: Arch Microbiol Source Type: research

Neorhizobium lilium sp. nov., an endophytic bacterium isolated from Lilium pumilum bulbs in Hebei province.
Abstract A novel gram-negative, aerobic, non-spore-forming, rod-shaped and non-nitrogen fixing bacterium named strain 24NRT was isolated from wild Lilium pumilum bulbs in Fuping, Baoding City, Hebei province, PR China. The 16S rRNA gene sequences of strains 24NRT showed the highest similarity to Neorhizobium alkalisoli DSM 21826T (98.5%) and N. galegae HAMBI 540T (98.1%). Phylogenetic analysis based on 16S rRNA genes and multilocus sequence analysis (MLSA) based on the partial sequences of atpD-glnII-glnA-recA-ropD-thrC housekeeping genes both indicated that strain 24NRT is a member of the genus Neorhizobium. The ...
Source: Archives of Microbiology - November 25, 2019 Category: Microbiology Authors: Liu L, Shi S, Liang L, Xu L, Chi M, Zhang X, Li L Tags: Arch Microbiol Source Type: research

Marinobacter nauticus (Baumann et al. 1972) comb. nov. arising from instances of synonymy and the incorrect interpretation of the International Code of Nomenclature of Prokaryotes.
Abstract Studies on Pseudomonas nautica Baumann et al. 1972 (Approved Lists 1980) and Marinobacter hydrocarbonoclasticus Gauthier et al. 1992 have shown that they should be treated as heterotypic synonyms. As a consequence, they have been treated as belonging to a single species, Marinobacter hydrocarbonoclasticus Gauthier et al. 1992. This interpretation of the International Code of Nomenclature of Bacteria/Prokaryotes is, however, based on a fundamental flaw in the interpretation of the wording of Rule 15 as documented in the 1975 and 1990 revisions where the wording has been partially corrected in the 2008 revi...
Source: Archives of Microbiology - November 23, 2019 Category: Microbiology Authors: Tindall BJ Tags: Arch Microbiol Source Type: research

Flavobacterium nackdongense sp. nov., a cellulose-degrading bacterium isolated from sediment.
Abstract A Gram-negative, non-motile, rod-shaped bacterial strain, designated GS13T, was isolated from sediments in a branch of the Nackdong River in Sangju, Korea. Optimal growth occurred at pH 7.0, 20 °C, and 0% NaCl. Phylogenetic analyses using 16S rRNA showed that strain GS13T is a member of the genus Flavobacterium, with highest similarity to Flavobacterium soyangense IMCC26223T (97.0%). The DNA G+C content of strain GS13T was 36.2 mol%. The dominant fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and iso-C15:0. The major polar lipids were phosphatidylethanolamine, three...
Source: Archives of Microbiology - November 19, 2019 Category: Microbiology Authors: Kim H, Yu SM Tags: Arch Microbiol Source Type: research

The clpX gene plays an important role in bacterial attachment, stress tolerance, and virulence in Xanthomonas campestris pv. campestris.
In this study, a clpX mutant was obtained by EZ-Tn5 transposon mutagenesis of the X. campestris pv. campestris. The clpX gene was annotated to encode ClpX, the ATP-binding subunit of ATP-dependent Clp protease. The clpX mutant exhibited reduced bacterial attachment, extracellular enzyme production and virulence. Mutation of clpX also resulted in increased sensitivity to a myriad of stresses, including heat, puromycin, and sodium dodecyl sulfate. These altered phenotypes of the clpX mutant could be restored to wild-type levels by in trans expression of the intact clpX gene. Proteomic analysis revealed that the expression of...
Source: Archives of Microbiology - November 19, 2019 Category: Microbiology Authors: Lo HH, Liao CT, Li CE, Chiang YC, Hsiao YM Tags: Arch Microbiol Source Type: research

Study of the sRNA RsmY involved in the genetic regulation of the synthesis of alginate and alkyl resorcinols in Azotobacter vinelandii.
e;ñez CE, Castañeda M Abstract Azotobacter vineladii is a Gram-negative bacterium that produces alginate and poly-hydroxybutyrate (PHB), two polymers of biotechnological interest. This bacterium has the ability to form desiccation-resistant cysts. In the cyst the membrane phospholipids are replaced with a family of phenolic lipids called alkylresorcinols (ARs). The alginate, PHB, and ARs are controlled by the GacS/A two-component system and the small regulatory RNA (sRNA) RsmZ1, belonging to the Rsm (Csr) regulatory system. The Rsm (Csr) systems usually possess two or more sRNAs, in this regard A. vi...
Source: Archives of Microbiology - November 19, 2019 Category: Microbiology Authors: López-Pliego L, Mena-Muñoz G, Terán-Melo JL, Fuentes LE, Núñez CE, Castañeda M Tags: Arch Microbiol Source Type: research

Screening of plant growth promoting attributes and arsenic remediation efficacy of bacteria isolated from agricultural soils of Chhattisgarh.
Abstract Arsenic (As) resistant indigenous bacteria with discrete minimum inhibitory concentration values for arsenate [As(V)] and arsenite [As(III)] were isolated from the paddy fields of different regions of Chhattisgarh, India, following enrichment culture technique. Evaluation of the plant growth promoting (PGP) properties of the isolates revealed that two rod-shaped Gram-positive bacteria viz., ARP2 and ART2 acquired various PGP traits, including phosphate solubilization, production of siderophore, indole acetic acid, ammonia, and exopolysaccharide. Both the isolates significantly increased (40-80%) the root ...
Source: Archives of Microbiology - November 18, 2019 Category: Microbiology Authors: Pandey N, Manjunath K, Sahu K Tags: Arch Microbiol Source Type: research

An innovative role for tenoxicam as a quorum sensing inhibitor in Pseudomonas aeruginosa.
In this study, the influence of tenoxicam, a non-steroidal anti-inflammatory drug, on quorum sensing in P. aeruginosa was characterized. Treatment of P. aeruginosa with tenoxicam decreased production of many virulence factors such as pyoverdin, rhamnolipids, pyocyanin, elastase, proteases, and hemolysins. Moreover, qRT-PCR revealed a significant reduction in expression of quorum sensing genes in tenoxicam-treated P. aeruginosa in comparison with untreated bacteria. Tenoxicam markedly reduced the capacity of P. aeruginosa to kill mice infection model. Mice injected with tenoxicam-treated P. aeruginosa exhibited higher survi...
Source: Archives of Microbiology - November 15, 2019 Category: Microbiology Authors: Askoura M, Saleh M, Abbas H Tags: Arch Microbiol Source Type: research

Bacterial communities in digestive and excretory organs of cicadas.
Abstract Bacteriocyte-associated symbionts are essential for the health of many sap-sucking insects, such as cicadas, leafhoppers and treehoppers, etc., but little is known about the bacterial community in the gut and other related organs in these insects. We characterized the bacterial communities in the salivary glands, alimentary canal and the Malpighian tubules of two populations of the cicada Subpsaltria yangi occurring in different habitats and feeding on different hosts. A high degree of similarity of core microbiota was revealed between the two populations, both with the top three bacteria belonging to Mei...
Source: Archives of Microbiology - November 13, 2019 Category: Microbiology Authors: Wang D, Wei C Tags: Arch Microbiol Source Type: research

The effect of temperature fluctuation on the microbial diversity and community structure of rural household biogas digesters at Qinghai Plateau.
Abstract Seasonal temperature-fluctuation has been regarded as a key environmental factor affecting rural biogas fermentation yields. The present study investigated the impact of seasonal temperature-fluctuation on operating-temperatures and biogas production in rural household digesters at Qinghai Plateau and revealed the related changes in microbial diversity and community structure by 16S rRNA gene high-throughput sequencing (HTS) analysis. Our results showed closely positive correlation between operating-temperatures and biogas production. HTS analysis indicated the highest diversity for bacteria community in ...
Source: Archives of Microbiology - November 11, 2019 Category: Microbiology Authors: Han R, Zhu D, Xing J, Li Q, Li Y, Chen L Tags: Arch Microbiol Source Type: research

Rototrichous: a new type of bacterial flagellation.
Abstract A rod-shaped microorganism with unknown type of flagellation has been accidentally discovered during phase-contrast microscopy of a sample of contaminated human donor blood. The flagellum consists of three fragments that form a complex locomotor device attached to bacterial body. The device provides bacterial motility by rotating around longitudinal axis of bacterial body and so this type of flagellation has been named "rototrichous." This newly discovered bacterial flagellation should be included in the classification of bacterial flagellations. PMID: 31712863 [PubMed - as supplied by publ...
Source: Archives of Microbiology - November 11, 2019 Category: Microbiology Authors: Minasyan H Tags: Arch Microbiol Source Type: research

Phylogenetic characteristics, virulence properties and antibiogram profile of motile Aeromonas spp. isolated from ornamental guppy (Poecilia reticulata).
Abstract Aeromonas spp. are opportunistic pathogenic bacteria related to an assembly of infectious diseases in ornamental fish. In the present study, virulence properties and antibiotic susceptibility of 52 guppy-borne Aeromonas spp. were investigated. The isolates were identified as A. veronii (n = 34), A. dhakensis (n = 10), A. hydrophila (n = 3), A. caviae (n = 3) and A. enteropelogenes (n = 2) by gyrB gene sequencing. The gyrB sequence deviation within and among the species ranged from 0 to 2.6% and 2.7-9.2%. Each species formed a distinct g...
Source: Archives of Microbiology - November 9, 2019 Category: Microbiology Authors: Hossain S, De Silva BCJ, Dahanayake PS, De Zoysa M, Heo GJ Tags: Arch Microbiol Source Type: research

Distinct patterns of microbial metabolic fingerprints in sows and their offspring: a pilot study.
Abstract Microbial metabolism and growth in the intestinal tract depend on the composition of substrates present in the digesta and their ability to be metabolised by the microorganisms. The aim of this pilot study was to characterise potential hindgut microbial activity during perinatal period in sows and their offspring. Rectal samples from three sows (1-3 weeks before and after birth) and three of their piglets (1-5 weeks after birth), were subjected to assays using BIOLOG GEN III microplates to produce metabolic fingerprints for each animal. The number of metabolised substrates of the sow hindgut mic...
Source: Archives of Microbiology - November 9, 2019 Category: Microbiology Authors: Grześkowiak Ł, Teske J, Zentek J, Vahjen W Tags: Arch Microbiol Source Type: research