Structure and biological activity in vitro of Flagellin and its mutants from Escherichia coli Nissle 1917
This study utilized AlphaFold2, SERS (Surface-enhanced Raman spectroscopy), and CD (circular dichroism) techniques to analyze the structural characteristics of FliCEcN and its mutants, FliCΔ174-506 and FliCΔ274-406, and tested their immune effects by stimulating Caco-2 cells in vitro. The results indicate that the D2 and D3 domains of FliCEcN have more complex interactions compared to the D1-D2 domain pair., and these domains also play a role in molecular docking with TLR5 (Toll-like receptor 5). Furthermore, FliCΔ274-406 has more missing side chain and characteristic amino acid peaks than FliCΔ174-506. The FliCEcN gro...
Source: Archives of Microbiology - April 18, 2024 Category: Microbiology Authors: Shuang Li Ming Wen Guilan Wen Ying Yang Source Type: research
Expression profiles and characterization of microRNAs responding to chitin in Arthrobotrys oligospora
Arch Microbiol. 2024 Apr 17;206(5):220. doi: 10.1007/s00203-024-03949-x.ABSTRACTExtracellular proteases, such as chitinases secreted by Arthrobotrys oligospora (A. oligospora), play a crucial role in the process of nematode infection. However, post-transcriptional regulation of gene expression involving microRNAs (miRNAs) in A. oligospora remains scarcely described. Hereto, transcriptome sequencing was carried out to analyze the expression profiles of chitin-responsive miRNAs in A. oligospora. Based on the RNA-seq data, the differential expression of miRNAs (DEmiRNAs) in response to chitin was screened, identified and char...
Source: Archives of Microbiology - April 17, 2024 Category: Microbiology Authors: Ningxing Li Yansen Sun Yucheng Liu Lixiang Wei Jiahua Zhang Nengxiu Li Dianming Sun Jian Jiao Yufei Zuo Ruobing Li Xuepeng Cai Jun Qiao Qingling Meng Source Type: research
Expression profiles and characterization of microRNAs responding to chitin in Arthrobotrys oligospora
Arch Microbiol. 2024 Apr 17;206(5):220. doi: 10.1007/s00203-024-03949-x.ABSTRACTExtracellular proteases, such as chitinases secreted by Arthrobotrys oligospora (A. oligospora), play a crucial role in the process of nematode infection. However, post-transcriptional regulation of gene expression involving microRNAs (miRNAs) in A. oligospora remains scarcely described. Hereto, transcriptome sequencing was carried out to analyze the expression profiles of chitin-responsive miRNAs in A. oligospora. Based on the RNA-seq data, the differential expression of miRNAs (DEmiRNAs) in response to chitin was screened, identified and char...
Source: Archives of Microbiology - April 17, 2024 Category: Microbiology Authors: Ningxing Li Yansen Sun Yucheng Liu Lixiang Wei Jiahua Zhang Nengxiu Li Dianming Sun Jian Jiao Yufei Zuo Ruobing Li Xuepeng Cai Jun Qiao Qingling Meng Source Type: research
A sulfated exopolysaccharide derived from Chlorella sp. exhibiting in vitro anti- α-D-Glucosidase activity
This study aimed to isolate a polysaccharide-producing microalga from an extreme ecosystem and to evaluate its capacity to inhibit the α-D-glucosidase enzyme. Chlorella strain is isolated from hypersaline Lake in the Algerian desert. The exopolysaccharide extraction was performed by the concentration of free-cell supernatant in a rotary evaporator. The infrared analysis showed a characteristic footprint of carbohydrates with particular functional groups, such as sulfate. Gas chromatography-mass spectrometry has revealed a hetero-exopolysaccharide composed of galactose 35.75%, glucose 21.13%, xylose 16.81%, fructose 6.96%,...
Source: Archives of Microbiology - April 16, 2024 Category: Microbiology Authors: Karima Guehaz Zakaria Boual Alia Telli Hicham Meskher Hakim Belkhalfa Guillaume Pierre Philippe Michaud Alessandra Adessi Source Type: research
Oral feed-based administration of phage cocktail protects rohu fish (Labeo rohita) against Aeromonas hydrophila infection
Arch Microbiol. 2024 Apr 16;206(5):219. doi: 10.1007/s00203-024-03951-3.ABSTRACTAeromonas hydrophila is one of the major freshwater fish pathogens. In the current study, a cocktail of D6 and CF7 phages was given orally to Labeo rohita to assess phage survival in fish organs as well as to determine the therapeutic efficacy of phage treatment against fish mortality caused by A. hydrophila. In the phage-coated feed, prepared by simple spraying method, phage counts were quite stable for up to 2 months with a decline of ≤ 0.23 log10 and ≤ 1.66 log10 PFU/g feed during 4 oC and room temperature storage. Throughout the experim...
Source: Archives of Microbiology - April 16, 2024 Category: Microbiology Authors: Sumeet Rai Anuj Tyagi Naveen Kumar B T Source Type: research
Molecular characterization of the nonstructural 5A (NS5A) region of hepatitis C virus in Thai blood donors
In conclusion, NS5A RASs at amino acid positions 30, 62, 54, 58, and 98 are present within HCV genotypes 3a and 1. While keeping in mind that additional information was not available on the anonymous blood donors tested in this study, these findings can contribute to understand the NS5A mutation. Further study with known patients under drug treatment is recommended.PMID:38619622 | DOI:10.1007/s00203-024-03950-4 (Source: Archives of Microbiology)
Source: Archives of Microbiology - April 15, 2024 Category: Microbiology Authors: Anchalee Sistayanarain Duangkamol Kunthalert Source Type: research
Exploring the potential of Aspergillus wentii: secondary metabolites and biological properties
Arch Microbiol. 2024 Apr 15;206(5):216. doi: 10.1007/s00203-024-03934-4.ABSTRACTFungi are of considerable importance due to their capacity to biosynthesize various secondary metabolites with bioactive properties that draw high attention in new drug discovery with beneficial uses for improving human well-being and life quality. Aspergillus genus members are widespread and cosmopolitan species with varying economic significance in the fields of industry, medicine, and agriculture. Its species are renowned for their biosynthesis of secondary metabolites, characterized by both potent biological activity and structural novelty,...
Source: Archives of Microbiology - April 15, 2024 Category: Microbiology Authors: Bayan E Ainousah Sabrin R M Ibrahim Abdulrahim A Alzain Shaimaa G A Mohamed Hazem G A Hussein Ahmed Ashour Hossam M Abdallah Gamal A Mohamed Source Type: research
Peptide based vaccine designing against endemic causing mammarenavirus using reverse vaccinology approach
This study of designing a peptide vaccine targeting the glycoprotein of mammarenavirues may help develop novel therapeutics in near future.PMID:38619666 | DOI:10.1007/s00203-024-03942-4 (Source: Archives of Microbiology)
Source: Archives of Microbiology - April 15, 2024 Category: Microbiology Authors: Dwaipayan Chaudhuri Joyeeta Datta Satyabrata Majumder Kalyan Giri Source Type: research
Molecular characterization of the nonstructural 5A (NS5A) region of hepatitis C virus in Thai blood donors
In conclusion, NS5A RASs at amino acid positions 30, 62, 54, 58, and 98 are present within HCV genotypes 3a and 1. While keeping in mind that additional information was not available on the anonymous blood donors tested in this study, these findings can contribute to understand the NS5A mutation. Further study with known patients under drug treatment is recommended.PMID:38619622 | DOI:10.1007/s00203-024-03950-4 (Source: Archives of Microbiology)
Source: Archives of Microbiology - April 15, 2024 Category: Microbiology Authors: Anchalee Sistayanarain Duangkamol Kunthalert Source Type: research
Exploring the potential of Aspergillus wentii: secondary metabolites and biological properties
Arch Microbiol. 2024 Apr 15;206(5):216. doi: 10.1007/s00203-024-03934-4.ABSTRACTFungi are of considerable importance due to their capacity to biosynthesize various secondary metabolites with bioactive properties that draw high attention in new drug discovery with beneficial uses for improving human well-being and life quality. Aspergillus genus members are widespread and cosmopolitan species with varying economic significance in the fields of industry, medicine, and agriculture. Its species are renowned for their biosynthesis of secondary metabolites, characterized by both potent biological activity and structural novelty,...
Source: Archives of Microbiology - April 15, 2024 Category: Microbiology Authors: Bayan E Ainousah Sabrin R M Ibrahim Abdulrahim A Alzain Shaimaa G A Mohamed Hazem G A Hussein Ahmed Ashour Hossam M Abdallah Gamal A Mohamed Source Type: research
Peptide based vaccine designing against endemic causing mammarenavirus using reverse vaccinology approach
This study of designing a peptide vaccine targeting the glycoprotein of mammarenavirues may help develop novel therapeutics in near future.PMID:38619666 | DOI:10.1007/s00203-024-03942-4 (Source: Archives of Microbiology)
Source: Archives of Microbiology - April 15, 2024 Category: Microbiology Authors: Dwaipayan Chaudhuri Joyeeta Datta Satyabrata Majumder Kalyan Giri Source Type: research
The biocontrol potentials of rhizospheric bacterium Bacillus velezensis K0T24 against mulberry bacterial wilt disease
In this study, through high-throughput sequencing, we compared the rhizosphere bacterial composition of the mulberry-resistant cultivar (K10) and susceptible cultivar (G12), confirming Bacillus as a genus-level biomarker for K10. Next, twelve Bacillus spp. isolates, derived from the rhizosphere of K10, were screened for their antagonistic activity against R. pseudosolanacearum. The isolate showing strong antagonism was identified as B. velezensis K0T24 and selected for further analysis. The fermentation supernatant of B. velezensis K0T24 significantly inhibited the growth of R. pseudosolanacearum (82.47%) and the expressio...
Source: Archives of Microbiology - April 14, 2024 Category: Microbiology Authors: Wenlian Jiao Ju Wen Na Li Ting Ou Changyu Qiu Yutong Ji Kai Lin Xiaojiao Liu Jie Xie Source Type: research
Biofilm inhibition/eradication: exploring strategies and confronting challenges in combatting biofilm
Arch Microbiol. 2024 Apr 14;206(5):212. doi: 10.1007/s00203-024-03938-0.ABSTRACTBiofilms are complex communities of microorganisms enclosed in a self-produced extracellular matrix, posing a significant threat to different sectors, including healthcare and industry. This review provides an overview of the challenges faced due to biofilm formation and different novel strategies that can combat biofilm formation. Bacteria inside the biofilm exhibit increased resistance against different antimicrobial agents, including conventional antibiotics, which can lead to severe problems in livestock and animals, including humans. In ad...
Source: Archives of Microbiology - April 14, 2024 Category: Microbiology Authors: Anmol Srivastava Nidhi Verma Vivek Kumar Pragati Apoorva Vishnu Agarwal Source Type: research
Role of auto-antibodies in the mechanisms of dengue pathogenesis and its progression: a comprehensive review
Arch Microbiol. 2024 Apr 15;206(5):214. doi: 10.1007/s00203-024-03954-0.ABSTRACTA complex interaction among virulence factors, host-genes and host immune system is considered to be responsible for dengue virus (DENV) infection and disease progression. Generation of auto-antibodies during DENV infection is a major phenomenon that plays a role in the pathophysiology of dengue hemorrhagic fever and dengue shock syndrome. Hemostasis, thrombocytopenia, hepatic endothelial dysfunction, and autoimmune blistering skin disease (pemphigus) are different clinical manifestations of dengue pathogenesis; produced due to the molecular mi...
Source: Archives of Microbiology - April 14, 2024 Category: Microbiology Authors: Tanusree Ghorai Avipsha Sarkar Anirban Roy Bijita Bhowmick Debadatta Nayak Satadal Das Source Type: research
Exploring laccase: a sustainable enzymatic solution for the paper recycling domain
Arch Microbiol. 2024 Apr 11;206(5):211. doi: 10.1007/s00203-024-03927-3.ABSTRACTThe global advocacy of resource conservation and waste management emphasizes the significance of sustainable practices, particularly in sectors such as paper manufacturing and recycling. Currently, conventional chemical methods are predominant for paper production, necessitating the use of substantial amount of toxic chemicals. This chemical-intensive approach compromises the recycled fiber quality, generates hazardous effluent causing serious ecological threats which triggers regulatory complexities for the mills. To address these challenges m...
Source: Archives of Microbiology - April 11, 2024 Category: Microbiology Authors: Sheetal Pandey Sarika Gupta Source Type: research
