Carbon substrates promotes stress resistance and drug tolerance in clinical isolates of Candida tropicalis
In this study, we determined the impact of glucose, fructose, and sucrose as the sole carbon source on the fluconazole resistance and osmotic (NaCl), oxidative (H2O2) stress adaptation in C. tropicalis clinical isolates. We confirmed that the abundance of carbon substrates influences or increases drug resistance and osmotic and oxidative stress tolerance in C. tropicalis. Additionally, both azole-resistant and susceptible isolates showed similar stress adaptation phenotypes, confirming the equal efficiency of becoming successful pathogens irrespective of drug susceptibility profile. To the best of our knowledge, our study ...
Source: Archives of Microbiology - May 20, 2024 Category: Microbiology Authors: Arpita Khamrai Saikat Paul Shivaprakash M Rudramurthy Anup K Ghosh Source Type: research

Secreted in Xylem (SIX) genes in Fusarium oxysporum f.sp. cubense (Foc) unravels the potential biomarkers for early detection of Fusarium wilt disease
Arch Microbiol. 2024 May 20;206(6):271. doi: 10.1007/s00203-024-03996-4.ABSTRACTSecreted in Xylem (SIX) are small effector proteins released by Fusarium oxysporum f.sp. cubense (Foc) into the plant's xylem sap disrupting the host's defence responses causing Fusarium wilt disease resulting in a significant decline in banana crop yields and economic losses. Notably, different races of Foc possess unique sets of SIX genes responsible for their virulence, however, these genes remain underutilized, despite their potential as biomarkers for early disease detection. Herein, we identified seven SIX genes i.e. SIX1, SIX2, SIX4, SIX...
Source: Archives of Microbiology - May 20, 2024 Category: Microbiology Authors: Kausalyaa Kaliapan Siti Nur Akmar Mazlin Kah Ooi Chua Nur Ardiyana Rejab Yusmin Mohd-Yusuf Source Type: research

Enterocin DD14 can inhibit the infection of eukaryotic cells with enveloped viruses
Arch Microbiol. 2024 May 20;206(6):269. doi: 10.1007/s00203-024-04002-7.ABSTRACTBacteriocins are ribosomally synthesized bacterial peptides endowed with antibacterial, antiprotozoal, anticancer and antiviral activities. In the present study, we evaluated the antiviral activities of two bacteriocins, enterocin DD14 (EntDD14) and lacticaseicin 30, against herpes simplex virus type 1 (HSV-1), human coronavirus 229E (HCoV-229E) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Vero, Huh7 and Vero E6 cells, respectively. In addition, the interactions of these bacteriocins with the envelope glycoprotein D of HS...
Source: Archives of Microbiology - May 20, 2024 Category: Microbiology Authors: Radja Teiar Famara Sane Ismail Erol Magloire Pandoua Nekoua Didier Lecouturier Rabah Boukherroub Serdar Durda ğı Didier Hober Djamel Drider Source Type: research

Carbon substrates promotes stress resistance and drug tolerance in clinical isolates of Candida tropicalis
In this study, we determined the impact of glucose, fructose, and sucrose as the sole carbon source on the fluconazole resistance and osmotic (NaCl), oxidative (H2O2) stress adaptation in C. tropicalis clinical isolates. We confirmed that the abundance of carbon substrates influences or increases drug resistance and osmotic and oxidative stress tolerance in C. tropicalis. Additionally, both azole-resistant and susceptible isolates showed similar stress adaptation phenotypes, confirming the equal efficiency of becoming successful pathogens irrespective of drug susceptibility profile. To the best of our knowledge, our study ...
Source: Archives of Microbiology - May 20, 2024 Category: Microbiology Authors: Arpita Khamrai Saikat Paul Shivaprakash M Rudramurthy Anup K Ghosh Source Type: research

Secreted in Xylem (SIX) genes in Fusarium oxysporum f.sp. cubense (Foc) unravels the potential biomarkers for early detection of Fusarium wilt disease
Arch Microbiol. 2024 May 20;206(6):271. doi: 10.1007/s00203-024-03996-4.ABSTRACTSecreted in Xylem (SIX) are small effector proteins released by Fusarium oxysporum f.sp. cubense (Foc) into the plant's xylem sap disrupting the host's defence responses causing Fusarium wilt disease resulting in a significant decline in banana crop yields and economic losses. Notably, different races of Foc possess unique sets of SIX genes responsible for their virulence, however, these genes remain underutilized, despite their potential as biomarkers for early disease detection. Herein, we identified seven SIX genes i.e. SIX1, SIX2, SIX4, SIX...
Source: Archives of Microbiology - May 20, 2024 Category: Microbiology Authors: Kausalyaa Kaliapan Siti Nur Akmar Mazlin Kah Ooi Chua Nur Ardiyana Rejab Yusmin Mohd-Yusuf Source Type: research

Enterocin DD14 can inhibit the infection of eukaryotic cells with enveloped viruses
Arch Microbiol. 2024 May 20;206(6):269. doi: 10.1007/s00203-024-04002-7.ABSTRACTBacteriocins are ribosomally synthesized bacterial peptides endowed with antibacterial, antiprotozoal, anticancer and antiviral activities. In the present study, we evaluated the antiviral activities of two bacteriocins, enterocin DD14 (EntDD14) and lacticaseicin 30, against herpes simplex virus type 1 (HSV-1), human coronavirus 229E (HCoV-229E) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Vero, Huh7 and Vero E6 cells, respectively. In addition, the interactions of these bacteriocins with the envelope glycoprotein D of HS...
Source: Archives of Microbiology - May 20, 2024 Category: Microbiology Authors: Radja Teiar Famara Sane Ismail Erol Magloire Pandoua Nekoua Didier Lecouturier Rabah Boukherroub Serdar Durda ğı Didier Hober Djamel Drider Source Type: research

Actinomycetes are a natural resource for sustainable pest control and safeguarding agriculture
Arch Microbiol. 2024 May 19;206(6):268. doi: 10.1007/s00203-024-03975-9.ABSTRACTActinomycetes, a diverse group of bacteria with filamentous growth characteristics, have long captivated researchers and biochemists for their prolific production of secondary metabolites. Among the myriad roles played by actinomycete secondary metabolites, their historical significance in the field of biocontrol stands out prominently. The fascinating journey begins with the discovery of antibiotics, where renowned compounds like streptomycin, tetracycline, and erythromycin revolutionized medicine and agriculture. The history of biocontrol tra...
Source: Archives of Microbiology - May 19, 2024 Category: Microbiology Authors: Mohamed Khaled Diab Hala Mohamed Mead Mohamad M Ahmad Khedr Abdelghafar Mohamed Abu-Elsaoud Sahar Ahmed El-Shatoury Source Type: research

Clostridium butyricum upregulates GPR109A/AMPK/PGC-1 α and ameliorates acute pancreatitis-associated intestinal barrier injury in mice
In conclusion, Clostridium butyricum could improve pancreatic and intestinal inflammation and pancreatic injury, and relieve acute pancreatitis-induced intestinal barrier damage in the small intestine and colon, which may be associated with GPR109A/AMPK/PGC-1α.PMID:38761195 | DOI:10.1007/s00203-024-04001-8 (Source: Archives of Microbiology)
Source: Archives of Microbiology - May 18, 2024 Category: Microbiology Authors: Guiqing Deng Biyan Wen Lin Jia Jiaxin Liu Qingqing Yan Source Type: research

A novel type IIb L-asparaginase from Latilactobacillus sakei LK-145: characterization and application
Arch Microbiol. 2024 May 18;206(6):266. doi: 10.1007/s00203-024-03979-5.ABSTRACTWe succeeded in homogeneously expressing and purifying L-asparaginase from Latilactobacillus sakei LK-145 (Ls-Asn1) and its mutated enzymes C196S, C264S, C290S, C196S/C264S, C196S/C290S, C264S/C290S, and C196S/C264S/C290S-Ls-Asn1. Enzymological studies using purified enzymes revealed that all cysteine residues of Ls-Asn1 were found to affect the catalytic activity of Ls-Asn1 to varying degrees. The mutation of Cys196 did not affect the specific activity, but the mutation of Cys264, even a single mutation, significantly decreased the specific ac...
Source: Archives of Microbiology - May 18, 2024 Category: Microbiology Authors: Shiro Kato Kazuya Tamura Yuki Masuda Morichika Konishi Kazuya Yamanaka Tadao Oikawa Source Type: research

Exploring the evolutionary and pathogenic role of Acinetobacter baumannii biofilm-associated protein (Bap) through in silico structural modeling
Arch Microbiol. 2024 May 18;206(6):267. doi: 10.1007/s00203-024-03992-8.ABSTRACTAcinetobacter species encode for extracellularly secreted Biofilm-associated protein (Bap), a multi-domain protein with variable molecular weights reaching several hundred kilodaltons. Bap is crucial for the development of multi-dimensional structures of mature biofilms. In our investigation, we analyzed 7338 sequences of A. baumannii from the NCBI database and found that Bap or Bap-like protein (BLP) was present in 6422 (87.52%) isolates. Further classification revealed that 12.12% carried Type-1 Bap, 68.44% had Type-2, 6.91% had Type-3, 0.05%...
Source: Archives of Microbiology - May 18, 2024 Category: Microbiology Authors: Kirti Upmanyu Rakesh Kumar Qazi Mohd Rizwanul Haque Ruchi Singh Source Type: research

Clostridium butyricum upregulates GPR109A/AMPK/PGC-1 α and ameliorates acute pancreatitis-associated intestinal barrier injury in mice
In conclusion, Clostridium butyricum could improve pancreatic and intestinal inflammation and pancreatic injury, and relieve acute pancreatitis-induced intestinal barrier damage in the small intestine and colon, which may be associated with GPR109A/AMPK/PGC-1α.PMID:38761195 | DOI:10.1007/s00203-024-04001-8 (Source: Archives of Microbiology)
Source: Archives of Microbiology - May 18, 2024 Category: Microbiology Authors: Guiqing Deng Biyan Wen Lin Jia Jiaxin Liu Qingqing Yan Source Type: research

A novel type IIb L-asparaginase from Latilactobacillus sakei LK-145: characterization and application
Arch Microbiol. 2024 May 18;206(6):266. doi: 10.1007/s00203-024-03979-5.ABSTRACTWe succeeded in homogeneously expressing and purifying L-asparaginase from Latilactobacillus sakei LK-145 (Ls-Asn1) and its mutated enzymes C196S, C264S, C290S, C196S/C264S, C196S/C290S, C264S/C290S, and C196S/C264S/C290S-Ls-Asn1. Enzymological studies using purified enzymes revealed that all cysteine residues of Ls-Asn1 were found to affect the catalytic activity of Ls-Asn1 to varying degrees. The mutation of Cys196 did not affect the specific activity, but the mutation of Cys264, even a single mutation, significantly decreased the specific ac...
Source: Archives of Microbiology - May 18, 2024 Category: Microbiology Authors: Shiro Kato Kazuya Tamura Yuki Masuda Morichika Konishi Kazuya Yamanaka Tadao Oikawa Source Type: research

Exploring the evolutionary and pathogenic role of Acinetobacter baumannii biofilm-associated protein (Bap) through in silico structural modeling
Arch Microbiol. 2024 May 18;206(6):267. doi: 10.1007/s00203-024-03992-8.ABSTRACTAcinetobacter species encode for extracellularly secreted Biofilm-associated protein (Bap), a multi-domain protein with variable molecular weights reaching several hundred kilodaltons. Bap is crucial for the development of multi-dimensional structures of mature biofilms. In our investigation, we analyzed 7338 sequences of A. baumannii from the NCBI database and found that Bap or Bap-like protein (BLP) was present in 6422 (87.52%) isolates. Further classification revealed that 12.12% carried Type-1 Bap, 68.44% had Type-2, 6.91% had Type-3, 0.05%...
Source: Archives of Microbiology - May 18, 2024 Category: Microbiology Authors: Kirti Upmanyu Rakesh Kumar Qazi Mohd Rizwanul Haque Ruchi Singh Source Type: research

Alkaliphilic and thermostable lipase production by leaf litter fungus Leptosphaerulina trifolii A SMR-2011
Arch Microbiol. 2024 May 18;206(6):264. doi: 10.1007/s00203-024-03997-3.ABSTRACTFungi that inhabit fire-prone forests have to be adapted to harsh conditions and fungi affiliated to Ascomycota recovered from foliar litter samples were used for bioprospecting of molecules such as enzymes. Agni's fungi isolated from leaf litter, whose spores are capable of tolerating 110 oC were screened for thermostable lipases. One of the isolates, Leptosphaerulina trifolii A SMR-2011 exhibited high positive lipase activity than other isolates while screening through agar plate assay using Tween 20 in the medium. Maximum lipase activity (17...
Source: Archives of Microbiology - May 17, 2024 Category: Microbiology Authors: Mondem Vasundhara Kirti Singh Trichur S Suryanarayanan Mondem Sudhakara Reddy Source Type: research

Heteroxylan hydrolysis by a recombinant cellulase-free GH10 xylanase from the alkaliphilic bacterium Halalkalibacterium halodurans C-125
In this study, a recombinant GH10 xylanase (Xyn10-HB) from the extremely alkaliphilic bacterium Halalkalibacterium halodurans C-125 cultivated at pH 10 was cloned and expressed in E. coli BL21(DE3). Removal of the signal peptide improved the expression, and an overall activity of 8 U/mL was obtained in the cell-free supernatant. The molecular weight of purified Xyn10-HB was estimated to be 42.6 kDa by SDS-PAGE. The enzyme was active across a wide pH range (5-10) with optimal activity recorded at pH 8.5 and 60 °C. It also presented good stability with a half-life of 3 h under these conditions. Substrate specificity studies...
Source: Archives of Microbiology - May 16, 2024 Category: Microbiology Authors: Jihene Maati Duarte Miguel Prazeres Marcin Gr ąz Adrian Wiater Anna Jarosz-Wilko łazka Issam Smaali Source Type: research