Comparison of utilizing GeneXpert MTB/RIF and other conventional methods on bronchoalveolar lavage in the diagnosis of Mycobacterium tuberculosis: A real-life retrospective study
This study is to evaluate the performance of GeneXpert MTB/RIF (GXP) assay versus Real-time PCR MTBC/NTM (Lytestar) and other conventional methods in the diagnosis of Mycobacterium tuberculosis (TB). We conducted a retrospective cross-sectional analysis of patients who were investigated for TB via bronchoalveolar lavage between March to December 2018 in a single tertiary pulmonary centre in Malaysia. Results of GXP were analyzed base on patient’s demographic characteristics and other diagnostic methods. 33 of total 72 patients were diagnosed with pulmonary tuberculosis either through rapid DNA testing via GXP or real...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Soo, C. I., Lim, K. Y., Rajah, R., Ng, B. H., Abdul Hamid, F., Ban, A. Y.-L. Tags: Tuberculosis Source Type: research

Indeterminate IFN-? release assay test results predict poor prognosis of active pulmonary tuberculosis
Conclusions: Indeterminate IGRA test results predict poor prognosis of active pulmonary tuberculosis.Adjusted odds ratio95% CIP-valueAge1.081.04 to 1.12
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Akamatsu, T., Shirai, T., Tamura, K., Masuda, T., Takahashi, S., Tanaka, Y., Kishimoto, Y., Oishi, K., Endo, Y., Saigusa, M., Yamamoto, A., Morita, S., Asada, K. Tags: Tuberculosis Source Type: research

The Pleural and blood gene expression level of GBP5 and STAT1 were associated with pleural tuberculosis: TB pleura diagnostic biomarkers
This study was aimed to investigate the TB-specific genes expression in pleural fluid and blood samples in settings of lymphocytic exudative pleural effusion.Methods: Each patient with lymphocytic exudative pleural effusion was asked to provide 3 ml of pleural fluid and 3 ml of blood samples, separately collected into the RNA collection tube, processed through the RNA isolation kit, used in the quantitative real-time PCR to measure the expression levels of 10 TB-specific genes (FCGR1B1, FCGR1B2, FCGR1A, MAFB, GBP5, APOL1, KCNJ15, STAT1, KAZN and CD53) and compared the level of each gene between TB pleura and MPE groups.Res...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Luekitinun, N., Mahasirimongkol, S., Satproedprai, N., Miyahara, R., Kawkitinarong, K. Tags: Tuberculosis Source Type: research

The change of PD-1 and CTLA-4 during LTBI and TB reactivation
Conclusions: We preliminarily found that the PD-1 expression were increasing during LTBI status and by contrast, CTLA-4 on CD4 lymphocytes as well as regulatory T cells elevated in TB reactivation. We suggest that the immune modulation pathways of PD-1 and CTLA-4 as well as Treg cells influence different stage of M. tuberculosis infection and needs further investigation for the application in treatment. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Shu, C.-C. Tags: Tuberculosis Source Type: research

Detection of NTM in surgery samples from patients hospitalized with presumed TB
TB and mycobacteriosis have similar clinical and radiological manifestations, which can result in a diagnostic error. Introduction of modern methods for diagnosing mycobacterial infections has allowed rapid and precise differentiation between TB and mycobacterioses. It is especially important for surgery patients.The aim of our study was to assess detection of NTM in surgery samples from patients on treatment in hospital at the Central TB Research Institute and assess a spectrum of NTM species.We studied 4440 surgery samples from 3423 patients in 2011-2018. Cultural diagnosis was performed in BACTEC MGIT 960, culture speci...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Smirnova, T., Ergeshov, A., Andreevskaya, S., Andrievskaya, I., Larionova, E., Chernousova, L. Tags: Tuberculosis Source Type: research

Clinical characteristics of pulmonary tuberculosis in elderly Japanese patients
Background: Japan is an aging society, and pulmonary tuberculosis (PTB) is likely to become an increasing problem in elderly patients.Aims and Objectives: The aim was to elucidate the current status and challenges of PTB in elderly patients.Methods: Elderly patients diagnosed with active PTB by culture from April 2006 to March 2017 were retrospectively reviewed. The patients were divided into those aged ≥75 years (elderly) and those aged
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Marukawa, M., Matsuoka, K., Higo, H., Suzaki, N., Nagata, T. Tags: Tuberculosis Source Type: research

Cough- and sputum-related quality of life is associated with the features of cavitation in pulmonary tuberculosis
Conclusions: Cough- and sputum-related QOL was associated with the features of cavitation in pulmonary TB.Table 1.Spearman’s correlation coefficients between cough- and sputum-related QOL and CT findings (*p
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Oishi, K., Shirai, T., Akamatsu, T., Suzuki, T., Nakayasu, H., Tamura, K., Masuda, T., Takahashi, S., Tanaka, Y., Kishimoto, Y., Endo, Y., Saigusa, M., Yamamoto, A., Morita, S., Asada, K. Tags: Tuberculosis Source Type: research

Side effects of tuberculosis chemotherapy in women of reproductive age: vaginal dysbacteriosis
Conclusions: aerobic anaerobic imbalance of vaginal microbiota is formed in patients of reproductive age on the background of TB chemotherapy. Therefore, women receiving intensive TB therapy should be included in the group of high risk on vaginal dysbacteriosis. They should receive preventive treatment with probiotics timely. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Kayukova, S., Lulueva, Z., Romanov, V., Ergeshov, A. Tags: Tuberculosis Source Type: research

Regional study on the management and coutcomes of Tuberculous Uveitis
In conclusion: Collaboration between physicians and opthalmologists positively influenced outcome of uveitis. 6 months of ATT may be sufficient to control TB uveitis. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Alderazi, S., Akpenyi, O., Hagan, G., Nathani, N. Tags: Tuberculosis Source Type: research

A Case Series of Pericardial TB in a large European Centre
Introduction: Pericardial Tuberculosis (TB) is a rare manifestation of TB disease in high-income countries (
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Sundaralingam, A., Kilic, Y., Burman, M., Rahman, A., White, V., Jayasekera, N., Darmalingam, M., Tiberi, S., Kunst, H. Tags: Tuberculosis Source Type: research

Antituberculosis drug- induced hepatitis, an unpredictable phenotype
Conclusion: Antituberculosis drug-induced hepatotoxicity is a complex phenotype, for which age, female gender and variants of NAT2 and ABCB11 genes, have a modest contribution. Moreover, different risk factors determine susceptibility to mild and severe liver damage. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: da Silva Alcobia, M. C., M Nogueira, L., Oliveiros, B., Robalo Cordeiro, C., C Silva, H. Tags: Tuberculosis Source Type: research

Utility of Xpert(R) MTB/RIF assay for extrapulmonary tuberculosis, a two year study
Conclusion: Xpert® MTB/RIF assay is an efficient, reliable and fast technique with good potential in rapid diagnosis of extrapulmonary tuberculosis in pyogenic body fluids. But in others (pleural fluid, ascitic fluid, CSF, gastric aspirate) it is not sensitive, efficient and cost-effective to be recommended for diagnosis of extrapulmonary tuberculosis.However, it significantly reduces the time compared to culture methods in making diagnosis and initiation of treatment. Therefore it can be used routinely in diagnosing extrapulmonary tuberculosis but there is still a scope of further research for more reliable, sensitive...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Agrawal, R., Agarwal, R., Gupta, U. Tags: Tuberculosis Source Type: research

The utility of Xpert MTB/RIF in the diagnosis of Tubercular Mediastinal Lymphadenopathy by Endobronchial Ultrasound
Conclusions: Xpert MTB/RIF using EBUS-TBNA samples is a useful laboratory test with good specificity in the diagnosis of tuberculosis. In high prevalence countries of Tuberculosis, Xpert MTB/RIF can decrease the diagnosis and treatment initiation time when combined with the conventional microscopy and histopathology (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Gupta, R., Bhatia, A., Joshi, S., Tayal, N., Pandey, P., Gupta, A. Tags: Tuberculosis Source Type: research

Sensitivity and Specificity of Cartridge Based Nucleic Acid Amplification Test in pleural biopsy obtained by thoracoscopy
Cartridge based nucleic acid amplification test (CBNAAT) is used widely to detect Mycobacterium Tuberculosis infection. It detects DNA sequences specific for Mycobacterium Tuberculosis and rifampicin resistance by polymerase chain reaction. The reults are available in two hours, making it the fastest method to detect Tuberculosis and rifampicin resistance. It has shown to have high sensitivity and specificty in smear positive pulmonary tuberculosis (98%and98% respectively) and a resonable result in smear negative cases (67%and99% respectively).Tubercular pleural effusion is the second most common extrapulmonary tuberculosi...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Muthreja, D., Swarnakar, R., Sontakke, A. Tags: Tuberculosis Source Type: research

Safety of bedaquiline for treatment of MDR/XDR tuberculosis in HIV co-infected patients
Conclusion: bedaquiline-containing regimens appear to show reassuring safety profile with low frequency of serious adverse reactions. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Galstyan, A., Borisov, S., Sinitsyn, M., Ivanova, D., Slogodskay, L. Tags: Tuberculosis Source Type: research

Mycobacterium tuberculosis detection rates by molecular genetic methods among patients with tuberculosis in the Russian Federation
Introduction: Molecular genetic methods (MGM) provide rapid detection of Mycobacterium tuberculosis (MTB) DNA and DST. It is critical important for TB and HIV co-infected patients due to acute and severe TB manifestation.Aims: To study efficiency of MTB DNA detection (MTBD) by MGM among new TB cases with HIV positive (HIV+) and HIV negative (HIV-) status.Methods: 9 TB hospitals from different Russian regions were randomly selected and 433 new TB cases, detected in 2018, were included in study: 115 HIV+, 318 HIV- patients. We used real-time PCR (Amplitub-qPCR) for MTBD. Detection rate was defined as the proportion of cases,...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Veselova, E., Panova, A., Kaminskiy, G., Testov, V., Samoilova, A., Vasilyeva, I. Tags: Tuberculosis Source Type: research

Drug resistance and HIV rates are high in Eastern European patients with Tuberculosis in a South East London Hospital
Conclusion: Young, male Eastern Europeans form a significant proportion of pulmonary INH-R/MDRTB/XDRTB, with high HIV and transfer of care rates. This may be representative of a working migrant population with a high risk of both initial and acquired TB resistance. Whole genome sequencing would help define this group further.Eastern European cohort (N=44)England 2016Europe 2016Male: Female2.31.41.5Site: Pulmonary36 (82%)54%76.3%HIV positive4/40 (10%)3.8%4.5%Positive microbiology/histology35 (80%)71%71%Drug susceptibility testing (n= 31)- INH- R- MDR/RR- XDR2 (6.4%)*1 (3.2%)*1 (3.2%)*5.5%1.7%0.2%-3.7%(20.1% of MDR were XDRC...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Ratnakumar, S., Choon, X. Y., Belton, M., Palchaudhuri, P., Cotton, L. Tags: Tuberculosis Source Type: research

The peculiarities of drug susceptibility of M. tuberculosis isolated from patients with late-stage HIV infection
Conclusions: Patients with late-stage HIV infection associated with newly diagnosed TB are predominantly infected with drug resistant M. tuberculosis (82.8%). The severest TB forms, which are extremely difficult to treat, are MDR, pre-XDR, and XDR TB. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Karpina, N., Dudchenko, A., Larionova, E., Ergeshov, A. Tags: Tuberculosis Source Type: research

Impact of seasons on tuberculosis mortality for HIV+ and HIV- TB patients
Background: Assessing the difference in the seasonality of death from TB can provide an answer to the question of whether the nature of the incidence of TB death cases in the presence or absence of HIV infection is different.Aims: Assessment of the presence of seasonal differences in mortality depending on the presence of HIV infectionMethods: TB associated monthly deaths of TB/HIV+ (926) and TB/HIV- (1050) patients in 2013-2018 were analyzed.The monthly data for 6 years were summed up for every month, and then compared with a theoretical uniform distribution of TB death frequency (TBDF).Results: For the TB/HIV- death case...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Chizhova, O., Belilovskiy, E., Borisov, S., Sinitsyn, M. Tags: Tuberculosis Source Type: research

Hospitalized tuberculosis: HIV versus non-HIV patients
Introduction: Tuberculosis (TB) clinical and epidemiologic characteristics can vary between HIV and non-HIV patients (NHIV).Aim: To analyze differences between HIV and NHIV patients in a hospital whose reference area has high TB incidence and high migrant population.Methods: Retrospective analysis of clinical and laboratorial data from hospitalized with TB patients for 5 years.Results: The study included 67 NHIV patients and 57 HIV patients. The majority was male (69% on NHIV and 81% on HIV) with a mean age of 44 in both groups. In HIV patients, 67% had CD4+ lymphocytes
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Martins de Matos Navarro Guia, M. F., Caixeiro, M., Boleo-Tome, J. P., Pacheco, P., Rodrigues, F. Tags: Tuberculosis Source Type: research

Burden of the primary drug resistance of Mycobacterium tuberculosis in HIV-positive patients
Conclusions: MDR-TB tuberculosis in comparison with HIV-negative pts. MDR TB was found 1.8 times more often among HIV-positive new TB pts. The causes of this phenomenon are to be investigated. We continue our research in the direction of estimation bacterial density in HIV+ and HIV- and MDR-TB clonal distribution in HIV affected areas. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Panova, A., Testov, V., Kaminskiy, G., Samoilova, A., Veselova, E., Vasilyeva, I. Tags: Tuberculosis Source Type: research

Clinical, immunological and epidemiological correlations of Tuberculosis / HIV Coinfection in patients Infected with HIV in Olt County in Romania between 2005-2015
Background: Global increase of people infected with HIV (PIH) is causing changes in tuberculosis (TB) clinical and epidemiological data. HIV infection increases up to 20 times the chance of progression from latent infection to active forms of TB.Purpose: The paper aims to assess patients with TB-HIV coinfection in Olt county of Romania registered in Regional Center for Monitoring and Evaluation of HIV/AIDS of Craiova (demographics, symptoms, immunologic evaluation, TB type, TB related number of treatments) in order to identify risk factors for progression to active TB in this subset of patients.Material and Method: We perf...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Calarasu, C., Nitu, F. M., Dumitrescu, F., Grecu, V. Tags: Tuberculosis Source Type: research

Treatment tactics in patients with tuberculous pleurisy
Conclusion: Use of laser therapy and phonophoresis with hydrocortisone in patients with tuberculous pleurisy accelerated recovery and minimized residual changes. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Chernyh, N., Tikhonova, M., Polyakova, A., Romanov, V., Amansahedov, R. Tags: Tuberculosis Source Type: research

Anti-tubercular drug concentrations in Pulmonary Tuberculosis patients - Diabetic vs Non-Diabetic groups
Conclusion: Pharmacokinetics of H and R between both the groups showed no significant difference. PTB-DM group showed trend towards poor clinical response, despite adequate plasma concentrations. Future studies may address higher ATT drug doses in Diabetics with a hope of improving clinical response. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Christopher, D. J., Jedidiah, D., Isaac, B., Mathew, B., Winston, B. Tags: Tuberculosis Source Type: research

Effectiveness of mesodiencephalic modulation in treatment of XDR TB patients with concomitant type II diabetes mellitus
Background: The progressive course of extensively drug resistant (XDR) TB accompanied by type II diabetes mellitus (DM) is associated with development of a chronic pathology with gradual degradation of neuroendocrine centres and reduced function of the hypothalamic-pituitary system.Aim: To evaluate effectiveness of mesodiencephalic modulation (MDM) in treatment of XDR TB/DM patients.Material and Methods: A prospective study of 68 XDR TB/DM patients in 2017-2019. Group 1 included 48 patients with progressive pulmonary TB (more than two lung lobes affected, destruction, massive bacterioexcretion). They received standard TB t...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Chernyh, N., Tikhonova, M., Romanov, V., Polyakova, A. Tags: Tuberculosis Source Type: research

Efficacy of treatment in MDR and XDR lung TB patients in the follow-up period with the use of new anti-tuberculosis agent
Introduction: Patients with XDR and MDR lung tuberculosis (TB) is the most severe contingent requiring the new drugs. Treatment efficacy of XDR and MDR TB is 28% - 58% (WHO, 2018).Aim: To compare treatment efficacy in XDR, MDR TB with the thioureidoiminomethylpyridinii perchlorate (Tpp) during the follow-up.Materials: 84 patients (18-70 years) with MDR and XDR TB were treated at TB department. I group (n = 37) with MDR and II group (n=47) with XDR TB were received Tpp in combination with Z + Cm + Lfx/ Ofl/ Mfx + Cs + E/Pto/PAS/Amox-Clv + Lzd/ Mpm to all patients according to the drug sensitivity for 6-8 months. Control exa...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Starshinova, A., Beliaeva, E., Nazarenko, M., Pavlova, M., Chernochaeva, I., Yablonskiy, P. Tags: Tuberculosis Source Type: research

Clinical relevance of differentiation between Mycobacterium avium and M. intracellulare in M. avium complex pulmonary disease in Croatia
Conclusions: M. avium showed a higher percentage of isolates meeting the MAC-PD criteria. Still, M.intracellulare disease was more severe in its presentation, which partly explains the higher rate and adequacy of treatment. Although one-year mortality of M. avium cases was doubled compared to M. intracellulare, there were no differences in long term survival between the two groups. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Glodic, G., Sabol, I., Zmak, L., Sola, A. M., Marusic, A., Hecimovic, A., Dzubur, F., Jakopovic, M., Kardum Bulat, L., Pavlisa, G., Samarzija, M., Civljak, A., Dakic, A., Jankovic Makek, M. Tags: Tuberculosis Source Type: research

The role of genexpert in the diagnosis of mycobacterium tuberculosis
Conclusion: GXP results are superior to smear microscopy and comparable to culture with shorter turn-around time. We recommend using it in routine TB diagnosis as this will expedite management of patients with presumptive TB. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Sasikumar, C., Utpat, K., Desai, U., Joshi, J. Tags: Tuberculosis Source Type: research

The significance of IP-10 level in blood serum for TB diagnosis in patients with late-stage HIV infection
Conclusion: The level of chemokine IP-10 in blood serum of more than 1236.64 pg/ml witnesses TB in patients with late-stage HIV infection. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Karpina, N., Dudchenko, A., Averbakh, M., Ergeshov, A. Tags: Tuberculosis Source Type: research

Interferon-gamma response by years after tuberculosis infection, measured with QuantiFERON-TB Plus
Conclusion: These results suggested that IFN- response might wane over 3 years after TB infection. The median of TB2-TB1 values in group 2 were zero, suggesting CD8+ T cells response might be weaker over 3 years after TB infection. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Takeda, K., Nagai, H., Suzukawa, M., Higa, K., Akashi, S., Kawashima, M., Ohshima, N., Yamane, A., Tamura, A., Matsui, H., Tohma, S. Tags: Tuberculosis Source Type: research

Efficacy of Matrix Metalloproteinase-9 in Differentiating Tuberculous from Non-Tuberculous Pleural Effusion
Conclusion: The matrix metalloproteinase-9 is a diagnostic test with good sensitivity and specificity. The mean level of matrix metalloproteinase-9 was lower in this study than other articles, which can affect the predictive value of this test.Keywords: pleural effusion, tuberculosis, matrix metalloproteinase-9 (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Moulaei, N. A., Niazi, A. A., Nouralahzadeh, M., Shahriyar, M. Tags: Tuberculosis Source Type: research

Role of CBNAAT in Extrapulmonary Tuberculosis- An ongoing pilot study
We examined 191 samples during the period. Pleural fluid was the most common sample(30.9%) collected followed by lymph node aspirate(24.6%). The overall sensitivity and specificity of CBNAAT was determined to be 26.77%(95% CI 19.31%-35.35%) and 100%(95% CI 94.40%-100%). The low sensitivity could be due to the low sample size and majority of the sample being pleural fluid. The sensitivity and specificity of CBNAAT in relation to mycobacterial culture, however, was 76.47%(95% CI 50.1%-93.19%) and 87.93%(95% CI 82.14%-92.37%) respectively.The sensitivity and specificity of CBNAAT was at par in comparison to mycobacterial cult...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Alwani, H., Subhankar, S., Rao, C. M., Dash, D. P. Tags: Tuberculosis Source Type: research

Prevention of adverse reactions due to pharmacotherapy in MRTB considering polymorphism of glutathione-S-transferase M1 and T1genes
Relevance: Genetics of widespread human diseases is a branch of research which is actively developing. The study of polymorphisms of known candidate genes and their prognostic impact on the disease, is one of the important tasks in the study of tuberculosis.Objective: To prevent the development of adverse reactions due to pharmacotherapy in multi-drug resistant tuberculosis considering deletion polymorphism of the genes in the detoxification system of glutathione-S-transferase xenobiotics.Subject: We have examined 100 patients with newly diagnosed multidrug-resistant uberculosis (MDR-TB).Methods: Polymorphism GSTM1 and GST...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Semianiv, I., Todoriko, L., Sprynsian, T., Yeremenchuk, I. Tags: Tuberculosis Source Type: research

Predicting the efficiency of the treatment of drug-resistant tuberculosis depending on the polymorphism variation of glutathione-S-transferase genes
Relevance: One of the main factors of the epidemic situation control regarding drug-resistant tuberculosis is effective treatment of patients because, in addition to curing a particular case.Objective: to determine the prognostic impact of the polymorphism of glutathione-S-transferase genes on the efficiency of treatment of drug-resistant tuberculosis.Methods: Polymorphism GSTM1 and GSTM1 areas were isolated using complex multi PCR for M. Arana et all (1996).Results: Patients with pulmonary XDR-TB who are carriers of the wild alleles (GSTM1 + / GSTT1 +) experienced the bacterioexcretion termination more frequently with the...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Semianiv, I., Todoriko, L., Sprynsian, T., Semianiv, M. Tags: Tuberculosis Source Type: research

Experimental justification choise to prevent hepatotoxic reactions to anti-TB drugs based on gene-phenotypic characteristics
To prevent the progression of hepatotoxic reaction in newly diagnosed patients with tuberculosis, various drugs of many groups are used.A benchmark assessment of the hepatoprotective effect of ropren, essentiale forte, ursosan, and heptral for rats with different acetylation phenotypes with liver damage caused by anti-tuberculosis drugs (ATD).Rats with a different acetylation phenotype were given the isoniazid, rifampicin, pyrazinamide, ethambutol daily for 2 weeks administered simultaneously with one of the hepatoprotectors: ropren, essentiale, ursodeoxycholic acid, ademetionin.The administration of the ATD led to the inc...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Kazakov, A., Mozhokina, G., Zyuzya, Y., Petrova, L. Tags: Tuberculosis Source Type: research

The value of immunological methods in the complex diagnostics of TB in chronic renal failure patients in the terminal stage
Conclusions: Immunological tests can be used in complex diagnostics of TB in patients with terminal stage of CRF, but their limitations should be taken into account. Low sensitivity of IGRAs can be explained by insufficient counts of Mtb-specific IFN--producingCD4 lymphocytes. Low specificity of the Mtb-CD27 can be attributed to pronounced inflammation associated with CRF (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Gordeeva, O., Telepnev, M., Nikitina, I., Shabalina, I., Chernousova, L., Karpina, N., Lyadova, I. Tags: Tuberculosis Source Type: research

Lung microRNAs as biomarkers of post primary TB variants
Introduction: A precondition for the development of post primary tuberculosis (TB) is the fault of natural resistance and specific immunity. Tuberculoma is a clinical form of pulmonary TB, combining various encapsulated caseous foci with a long and often low-symptom course. Fibrouscavernous pulmonary TB (FCT) is a chronic form characterized by the presence of a fibrous cavity, the development of fibrotic changes in the lung tissue and foci of bronchogenic dropout. Considering their role as regulators of immune response, microRNAs might play major part in the outcome of TB.Aim: We investigated expression of miRNAs in lung t...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Shepelkova, G., Evstifeev, V., Ergesheva, A., Guskov, A., Bagirov, M., Yeremeev, V. Tags: Tuberculosis Source Type: research

Diagnostic performance of antigen induced IFN-? production in pleural fluid in tuberculous pleurisy
Conclusion: Assessment of IFN- is more reliable for the diagnosis of tuberculosis pleurisy than standard tests. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Kosareva, O., Skornyakov, S., Tsvirenko, S., Fadina, O., Karskanova, S., Melyakh, S., Lugovkina, T. Tags: Tuberculosis Source Type: research

Tuberculosis Antigen Stimulated CXCR3 Ligand Assay for the Diagnosis of Tuberculous Lymphadenitis
Conclusion: TB antigen stimulated assays of I-TAC and MIG may be useful surrogate markers in the diagnosis of tuberculous lymphadenopathy (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Chung, W. Y., Jung, Y. J., Park, J. E., Sheen, S. S., Lee, K. S., Park, K. J. Tags: Tuberculosis Source Type: research

Autoantibodies to vimentin different modifications in patients with tuberculosis and different granulomatous lung diseases
Conclusions: High concentration of anti-MCV is more common for patients with TB, while anti-CCP has no significance for all studied lung diseases. Absence of anti-CCP can be evidence, that citrullination of vimentin is not necessary for development of autoimmune reaction in tuberculosis patients. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Malkova, A., Starshinova, A., Lapin, S., Surkova, E., Zinchenko, J., Basantsova, N., Gildburd, B., Yablonskiy, P. Tags: Tuberculosis Source Type: research

Relationship of interleukin 10 1082 G/A gene polymorphism with the duration of nephrotoxic onset due to anti tuberculosis drugs in multi drug resistant tuberculosis patients
Conclusion: IL-10 1082 G/A gene polymorphism was associated with long onset of nephrotoxic therapy with kanamycin in the management of MDR-TB.Keywords: MDR-TB, IL-10 1082 G/A, nephrotoxic gene polymorphism. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Harsini, H., Umarudin, U., Aphridasari, J. Tags: Tuberculosis Source Type: research

Contribution of invasive methods in the diagnosis of pulmonary tuberculosis
Introduction: The diagnosis of pulmonary tuberculosis(PT) is based on clinical and radiological arguments but a microbiologic or histologic proof is a gold standard. Bronchoscopic procedures, biopsy techniques and conventional surgery could play a primary role in PT diagnosis, particularly in patients with scarce sputum or negative sputum smearAim: Emphasize contribution of invasive techniques in the diagnosis of PTMethods: Retrospective study(2011-2018)including patients with PT and treated according to WHO recommendations in pulmonary department C(Mami hospital in Tunisia). Population was divided into two groups:G1(n=112...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Slim, A., Daghfous, H., Ben Mansour, A., Ezzaouia, A., Kchouk, H., Ben Saad, S., Tritar, F. Tags: Tuberculosis Source Type: research

Genetic Determinants of Drug Resistance in Mycobacterium tuberculosis Beijing B0/W148 isolates
Conclusion: We identified the high level of MDR pre-XDR and XDR associated mutations among Beijing B0/W148 clone that underlines the epidemiological and clinical danger of this genetic variant of MTB. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Umpeleva, T., Belousova, K., Golubeva, L., Eremeeva, N., Vakhrusheva, D. Tags: Tuberculosis Source Type: research

Estimation of resistance of M.tuberculosis different genotypes to disinfectants
To prevent the speeding on the nosocomial TB this are widely used chemical disinfectants.Aim: Estimation of resistance of Mtb different genotypes to disinfectants.Material and Methods: 30 multidrug-resistant cultures of Mtb: 10 isolates each of genotypes Beijing, LAM, Ural, isolated from patients. Disinfectants: solutions of quaternary ammonium compounds (QACs) with a concentration of active substance: 0.075-0.26%; solutions of hydrogen peroxide with peracid with a concentration of active substance: 0.05-1.1%. Mtb resistance to disinfectants was estimated following the UNE-EN 14348 standard and tissue test-object method (e...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Eremeeva, N., Umpeleva, T., Belousova, K., Vakhrusheva, D., Kanichev, V., Lavrechuk, L. Tags: Tuberculosis Source Type: research

Benchtop NMR-based metabolomic analysis as a diagnostic tool for tuberculosis in clinical urine samples
This study aimed at developing a Nuclear Magnetic Resonance (NMR)-based metabolomic approach for the differential diagnosis of TB from urine samples. Translational potential has been also proved by the use of an affordable and portable low-field (LF) NMR spectrometer.Methods: Urine samples from adult patients diagnosed of TB (n=19), other respiratory infection (n=25) and healthy controls (n=29) were examined using a Bruker Avance high-field (HF) (16.4T) spectrometer and a Magritek Spinsolve LF (1.4T) spectrometer. Principal Component Analysis (PCA) was applied to identify metabolic differences between groups. Classificator...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Izquierdo-Garcia, J. L., Comella-Del-Barrio, P., Campos-Olivas, R., Casanova, F., Dominguez, J., Ruiz-Cabello, J. Tags: Tuberculosis Source Type: research

Predicted factors of radiographic sequelae in tuberculosis treated patients
Background: Despite an efficient cure, tuberculosis (TB) is a global cause of mortality and morbidity. Patients with treated TB may suffer from sequelae, impairing their quality of life.Aim: Assess the predictive factors linked with the occurrence of sequelae among TB treated patients.Methods: A retrospective (2010-2017) study that included patients with pulmonary tuberculosis (TB) in Pulmonology department C of Abderrahmen Mami Hospital in Tunisia. Sequelae were defined by the persistance of radiographic abnormalities in the chest X-ray after a well conducted treatment. Patients were divided in 2 groups. G1 : patients wit...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Ezzaouia, A., Daghfous, H., Ben Mansour, A., Slim, A., Kchouk, H., Ben Saad, S., Tritar, F. Tags: Tuberculosis Source Type: research

Cumulative incidence and associated risk factor of chronic pulmonary infection after lung resection surgery for lung cancer
This study is to analyzed Cumulative incidence and associated risk factor of chronic pulmonary infection after lung resection surgery for lung cancerWe retrospectively analyze cumulative incidence and associated risk factor of chronic pulmonary infection for total of 3491 patients who was operated lung resection surgery for lung cancer from January 2010 to December 2014Of the 3,491 patients who underwent surgery for lung cancer, 123 developed chronic pulmonary infection (29 pulmonary tuberculosis, 47 non-tuberculous mycobacterial lung disease, 56 chronic pulmonary aspergillosis, 1 pulmonary actinomycosis). The cumulative i...
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Kang, J., Lee, K., Um, S.-W., Kim, H., Kown, O. J., Choi, Y. S., Jeong, B.-H. Tags: Lung cancer Source Type: research

Etiology of mediastinal lymph node enlargement in patients who underwent EBUS-TBNA
Conclusions: The most common cause of ML were malignant diseases. Sarcoidosis and HF accounted for the majority of benign ML causes, while infections only occasionally were diagnosed as the cause of ML. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Dabrowska, M., Faber, K., Tandejko-Burdyna, M., Korczynski, P., Krenke, R. Tags: Pleural and mediastinal malignancies Source Type: research

Clinical performance of a 6 mm mini rigid thoracoscope for diagnostic and therapeutic medical thoracoscopy
Conclusion: MRT is safe and effective with procedural yield of 100% and diagnostic yield of 90.78%. MRT Can also be used for therapeutic ahdesiolysis and talc poudrage. (Source: European Respiratory Journal)
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Vaddepally, C., Peddi, S., Maturu, N. Tags: Interventional pulmonology Source Type: research

Efficacy evaluation, time estimates for method of bronchial valve closure (BVC) in the treatment of patients with double infections of immunodeficiency virus / tuberculosis (HI virus/TB)
Results: All patients with HI virus according to the indicator of CD4+ cell lymphosytes were divided into 3 groups:≤100-52%,
Source: European Respiratory Journal - November 21, 2019 Category: Respiratory Medicine Authors: Iurev, D., Smerdin, S., Toschevikov, M., Akimova, D. Tags: Interventional pulmonology Source Type: research