Introducing noncanonical amino acids for studying and engineering bacterial microcompartments
Curr Opin Microbiol. 2021 Apr 1;61:67-72. doi: 10.1016/j.mib.2021.03.004. Online ahead of print.ABSTRACTBacterial microcompartments (BMCs) with selectively permeable shells and encapsulated enzyme cores are well-suited candidates for nano-bioreactors because of their advantages of enhancing pathway flux and protection against toxic products. To better study and engineer protein-based BMCs, a series of protein chemistry approaches are adopted. As one of the most advanced techniques, genetic code expansion can introduce various noncanonical amino acids (ncAAs) with diverse functional groups into target proteins, thus providi...
Source: Current Opinion in Microbiology - April 4, 2021 Category: Microbiology Authors: Hao Chen Jessica Wilson Sara Ottinger Qinglei Gan Chenguang Fan Source Type: research

Introducing noncanonical amino acids for studying and engineering bacterial microcompartments
Curr Opin Microbiol. 2021 Apr 1;61:67-72. doi: 10.1016/j.mib.2021.03.004. Online ahead of print.ABSTRACTBacterial microcompartments (BMCs) with selectively permeable shells and encapsulated enzyme cores are well-suited candidates for nano-bioreactors because of their advantages of enhancing pathway flux and protection against toxic products. To better study and engineer protein-based BMCs, a series of protein chemistry approaches are adopted. As one of the most advanced techniques, genetic code expansion can introduce various noncanonical amino acids (ncAAs) with diverse functional groups into target proteins, thus providi...
Source: Current Opinion in Microbiology - April 4, 2021 Category: Microbiology Authors: Hao Chen Jessica Wilson Sara Ottinger Qinglei Gan Chenguang Fan Source Type: research

Introducing noncanonical amino acids for studying and engineering bacterial microcompartments
Curr Opin Microbiol. 2021 Apr 1;61:67-72. doi: 10.1016/j.mib.2021.03.004. Online ahead of print.ABSTRACTBacterial microcompartments (BMCs) with selectively permeable shells and encapsulated enzyme cores are well-suited candidates for nano-bioreactors because of their advantages of enhancing pathway flux and protection against toxic products. To better study and engineer protein-based BMCs, a series of protein chemistry approaches are adopted. As one of the most advanced techniques, genetic code expansion can introduce various noncanonical amino acids (ncAAs) with diverse functional groups into target proteins, thus providi...
Source: Current Opinion in Microbiology - April 4, 2021 Category: Microbiology Authors: Hao Chen Jessica Wilson Sara Ottinger Qinglei Gan Chenguang Fan Source Type: research

Introducing noncanonical amino acids for studying and engineering bacterial microcompartments
Curr Opin Microbiol. 2021 Apr 1;61:67-72. doi: 10.1016/j.mib.2021.03.004. Online ahead of print.ABSTRACTBacterial microcompartments (BMCs) with selectively permeable shells and encapsulated enzyme cores are well-suited candidates for nano-bioreactors because of their advantages of enhancing pathway flux and protection against toxic products. To better study and engineer protein-based BMCs, a series of protein chemistry approaches are adopted. As one of the most advanced techniques, genetic code expansion can introduce various noncanonical amino acids (ncAAs) with diverse functional groups into target proteins, thus providi...
Source: Current Opinion in Microbiology - April 4, 2021 Category: Microbiology Authors: Hao Chen Jessica Wilson Sara Ottinger Qinglei Gan Chenguang Fan Source Type: research

Introducing noncanonical amino acids for studying and engineering bacterial microcompartments
Curr Opin Microbiol. 2021 Apr 1;61:67-72. doi: 10.1016/j.mib.2021.03.004. Online ahead of print.ABSTRACTBacterial microcompartments (BMCs) with selectively permeable shells and encapsulated enzyme cores are well-suited candidates for nano-bioreactors because of their advantages of enhancing pathway flux and protection against toxic products. To better study and engineer protein-based BMCs, a series of protein chemistry approaches are adopted. As one of the most advanced techniques, genetic code expansion can introduce various noncanonical amino acids (ncAAs) with diverse functional groups into target proteins, thus providi...
Source: Current Opinion in Microbiology - April 4, 2021 Category: Microbiology Authors: Hao Chen Jessica Wilson Sara Ottinger Qinglei Gan Chenguang Fan Source Type: research

New discoveries expand possibilities for carboxysome engineering
Curr Opin Microbiol. 2021 Mar 30;61:58-66. doi: 10.1016/j.mib.2021.03.002. Online ahead of print.ABSTRACTCarboxysomes are CO2-fixing protein compartments present in all cyanobacteria and some proteobacteria. These structures are attractive candidates for carbon assimilation bioengineering because they concentrate carbon, allowing the fixation reaction to occur near its maximum rate, and because they self-assemble in diverse organisms with a set of standard biological parts. Recent discoveries have expanded our understanding of how the carboxysome assembles, distributes itself, and sustains its metabolism. These studies hav...
Source: Current Opinion in Microbiology - April 2, 2021 Category: Microbiology Authors: Julia S Borden David F Savage Source Type: research

New discoveries expand possibilities for carboxysome engineering
Curr Opin Microbiol. 2021 Mar 30;61:58-66. doi: 10.1016/j.mib.2021.03.002. Online ahead of print.ABSTRACTCarboxysomes are CO2-fixing protein compartments present in all cyanobacteria and some proteobacteria. These structures are attractive candidates for carbon assimilation bioengineering because they concentrate carbon, allowing the fixation reaction to occur near its maximum rate, and because they self-assemble in diverse organisms with a set of standard biological parts. Recent discoveries have expanded our understanding of how the carboxysome assembles, distributes itself, and sustains its metabolism. These studies hav...
Source: Current Opinion in Microbiology - April 2, 2021 Category: Microbiology Authors: Julia S Borden David F Savage Source Type: research

Structure-based design of novel polyhedral protein nanomaterials
Curr Opin Microbiol. 2021 Mar 27;61:51-57. doi: 10.1016/j.mib.2021.03.003. Online ahead of print.ABSTRACTOrganizing matter at the atomic scale is a central goal of nanotechnology. Bottom-up approaches, in which molecular building blocks are programmed to assemble via supramolecular interactions, are a proven and versatile route to new and useful nanomaterials. Although a wide variety of molecules have been used as building blocks, proteins have several intrinsic features that present unique opportunities for designing nanomaterials with sophisticated functions. There has been tremendous recent progress in designing protein...
Source: Current Opinion in Microbiology - March 30, 2021 Category: Microbiology Authors: Alena Khmelinskaia Adam Wargacki Neil P King Source Type: research

Structure-based design of novel polyhedral protein nanomaterials
Curr Opin Microbiol. 2021 Mar 27;61:51-57. doi: 10.1016/j.mib.2021.03.003. Online ahead of print.ABSTRACTOrganizing matter at the atomic scale is a central goal of nanotechnology. Bottom-up approaches, in which molecular building blocks are programmed to assemble via supramolecular interactions, are a proven and versatile route to new and useful nanomaterials. Although a wide variety of molecules have been used as building blocks, proteins have several intrinsic features that present unique opportunities for designing nanomaterials with sophisticated functions. There has been tremendous recent progress in designing protein...
Source: Current Opinion in Microbiology - March 30, 2021 Category: Microbiology Authors: Alena Khmelinskaia Adam Wargacki Neil P King Source Type: research

Structure-based design of novel polyhedral protein nanomaterials
Curr Opin Microbiol. 2021 Mar 27;61:51-57. doi: 10.1016/j.mib.2021.03.003. Online ahead of print.ABSTRACTOrganizing matter at the atomic scale is a central goal of nanotechnology. Bottom-up approaches, in which molecular building blocks are programmed to assemble via supramolecular interactions, are a proven and versatile route to new and useful nanomaterials. Although a wide variety of molecules have been used as building blocks, proteins have several intrinsic features that present unique opportunities for designing nanomaterials with sophisticated functions. There has been tremendous recent progress in designing protein...
Source: Current Opinion in Microbiology - March 30, 2021 Category: Microbiology Authors: Alena Khmelinskaia Adam Wargacki Neil P King Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

Diversity of bacterial chemosensory systems
Curr Opin Microbiol. 2021 Mar 5;61:42-50. doi: 10.1016/j.mib.2021.01.016. Online ahead of print.ABSTRACTChemosensory system is the most complex, specialized mode of signal transduction in bacteria and archaea. It is composed of several core and auxiliary protein components that are highly organized in order to deliver a fast response to changing environmental conditions. Chemosensory pathways were studied in-depth in a handful of model organisms and experimentally characterized at least to some degree in approximately thirty other species. However, genome-wide analyses have revealed their presence in thousands of sequenced...
Source: Current Opinion in Microbiology - March 8, 2021 Category: Microbiology Authors: Vadim M Gumerov Ekaterina P Andrianova Igor B Zhulin Source Type: research

When the metabolism meets the cell cycle in bacteria
Curr Opin Microbiol. 2021 Mar 4;60:104-113. doi: 10.1016/j.mib.2021.02.006. Online ahead of print.ABSTRACTNutrients availability is the sinews of the war for single microbial cells, driving growth and cell cycle progression. Therefore, coordinating cellular processes with nutrients availability is crucial, not only to survive upon famine or fluctuating conditions but also to rapidly thrive and colonize plentiful environments. While metabolism is traditionally seen as a set of chemical reactions taking place in cells to extract energy and produce building blocks from available nutrients, numerous connections between metabol...
Source: Current Opinion in Microbiology - March 7, 2021 Category: Microbiology Authors: Fran çois Beaufay J érôme Coppine R égis Hallez Source Type: research

When the metabolism meets the cell cycle in bacteria
Curr Opin Microbiol. 2021 Mar 4;60:104-113. doi: 10.1016/j.mib.2021.02.006. Online ahead of print.ABSTRACTNutrients availability is the sinews of the war for single microbial cells, driving growth and cell cycle progression. Therefore, coordinating cellular processes with nutrients availability is crucial, not only to survive upon famine or fluctuating conditions but also to rapidly thrive and colonize plentiful environments. While metabolism is traditionally seen as a set of chemical reactions taking place in cells to extract energy and produce building blocks from available nutrients, numerous connections between metabol...
Source: Current Opinion in Microbiology - March 7, 2021 Category: Microbiology Authors: Fran çois Beaufay J érôme Coppine R égis Hallez Source Type: research

Cell-cell communication through septal junctions in filamentous cyanobacteria
Curr Opin Microbiol. 2021 Mar 3;61:35-41. doi: 10.1016/j.mib.2021.02.002. Online ahead of print.ABSTRACTSeptal junctions are cell-cell connections that mediate intercellular communication in filamentous cyanobacteria. The septal peptidoglycan is perforated by dozens of 20 nm-wide nanopores, through which these proteinaceous structures traverse, physically connecting adjacent cells. On each cytoplasmic side, every septal junction contains a flexible cap structure that closes the connection in a reversible manner upon stress. This gating mechanism reminds of the gap junctions from metazoans and represents a primordial contro...
Source: Current Opinion in Microbiology - March 6, 2021 Category: Microbiology Authors: Ann-Katrin Kieninger Iris Maldener Source Type: research

Lipoproteins in Gram-negative bacteria: new insights into their biogenesis, subcellular targeting and functional roles
Curr Opin Microbiol. 2021 Mar 2;61:25-34. doi: 10.1016/j.mib.2021.02.003. Online ahead of print.ABSTRACTBacterial lipoproteins are globular proteins anchored to a membrane by a lipid moiety. By discovering new functions carried out by lipoproteins, recent research has highlighted the crucial roles played by these proteins in the cell envelope of Gram-negative bacteria. Here, after discussing the wide range of activities carried out by lipoproteins in the model bacterium Escherichia coli, we review new insights into the essential mechanisms involved in lipoprotein maturation, sorting and targeting to their final destination...
Source: Current Opinion in Microbiology - March 5, 2021 Category: Microbiology Authors: Jessica El Rayes Raquel Rodr íguez-Alonso Jean-Fran çois Collet Source Type: research

Lipoproteins in Gram-negative bacteria: new insights into their biogenesis, subcellular targeting and functional roles
Curr Opin Microbiol. 2021 Mar 2;61:25-34. doi: 10.1016/j.mib.2021.02.003. Online ahead of print.ABSTRACTBacterial lipoproteins are globular proteins anchored to a membrane by a lipid moiety. By discovering new functions carried out by lipoproteins, recent research has highlighted the crucial roles played by these proteins in the cell envelope of Gram-negative bacteria. Here, after discussing the wide range of activities carried out by lipoproteins in the model bacterium Escherichia coli, we review new insights into the essential mechanisms involved in lipoprotein maturation, sorting and targeting to their final destination...
Source: Current Opinion in Microbiology - March 5, 2021 Category: Microbiology Authors: Jessica El Rayes Raquel Rodr íguez-Alonso Jean-Fran çois Collet Source Type: research

Natural products that target the cell envelope
Curr Opin Microbiol. 2021 Mar 1;61:16-24. doi: 10.1016/j.mib.2021.02.001. Online ahead of print.ABSTRACTThe inexorable spread of resistance to clinically used drugs demands that we maintain a full pipeline of antibiotic candidates. As organisms have struggled to survive and compete over evolutionary history, they have developed the capacity to make a remarkably diverse array of natural products that target the cell envelope. A few have been developed for use in the clinic but most have not, and there are still an enormous number of opportunities to investigate. Substrate-binding antibiotics for Gram-positive organisms, pha...
Source: Current Opinion in Microbiology - March 4, 2021 Category: Microbiology Authors: Julia E Page Suzanne Walker Source Type: research

Natural products that target the cell envelope
Curr Opin Microbiol. 2021 Mar 1;61:16-24. doi: 10.1016/j.mib.2021.02.001. Online ahead of print.ABSTRACTThe inexorable spread of resistance to clinically used drugs demands that we maintain a full pipeline of antibiotic candidates. As organisms have struggled to survive and compete over evolutionary history, they have developed the capacity to make a remarkably diverse array of natural products that target the cell envelope. A few have been developed for use in the clinic but most have not, and there are still an enormous number of opportunities to investigate. Substrate-binding antibiotics for Gram-positive organisms, pha...
Source: Current Opinion in Microbiology - March 4, 2021 Category: Microbiology Authors: Julia E Page Suzanne Walker Source Type: research

PAS domains in bacterial signal transduction
Curr Opin Microbiol. 2021 Feb 26;61:8-15. doi: 10.1016/j.mib.2021.01.004. Online ahead of print.ABSTRACTPAS domains are widespread, versatile domains found in proteins from all kingdoms of life. The PAS fold is composed of an antiparallel β-sheet with several flanking α-helices, and contains a conserved cleft for cofactor or ligand binding. The last few years have seen a prodigious increase in identified PAS domains and resolved PAS structures, including structures with effector and other domains. New bacterial PAS ligands have been discovered, and structure-function studies have improved our understanding of PA...
Source: Current Opinion in Microbiology - March 1, 2021 Category: Microbiology Authors: Erwin C Stuffle Mark S Johnson Kylie J Watts Source Type: research

PAS domains in bacterial signal transduction
Curr Opin Microbiol. 2021 Feb 26;61:8-15. doi: 10.1016/j.mib.2021.01.004. Online ahead of print.ABSTRACTPAS domains are widespread, versatile domains found in proteins from all kingdoms of life. The PAS fold is composed of an antiparallel β-sheet with several flanking α-helices, and contains a conserved cleft for cofactor or ligand binding. The last few years have seen a prodigious increase in identified PAS domains and resolved PAS structures, including structures with effector and other domains. New bacterial PAS ligands have been discovered, and structure-function studies have improved our understanding of PA...
Source: Current Opinion in Microbiology - March 1, 2021 Category: Microbiology Authors: Erwin C Stuffle Mark S Johnson Kylie J Watts Source Type: research

PAS domains in bacterial signal transduction
Curr Opin Microbiol. 2021 Feb 26;61:8-15. doi: 10.1016/j.mib.2021.01.004. Online ahead of print.ABSTRACTPAS domains are widespread, versatile domains found in proteins from all kingdoms of life. The PAS fold is composed of an antiparallel β-sheet with several flanking α-helices, and contains a conserved cleft for cofactor or ligand binding. The last few years have seen a prodigious increase in identified PAS domains and resolved PAS structures, including structures with effector and other domains. New bacterial PAS ligands have been discovered, and structure-function studies have improved our understanding of PA...
Source: Current Opinion in Microbiology - March 1, 2021 Category: Microbiology Authors: Erwin C Stuffle Mark S Johnson Kylie J Watts Source Type: research

Integrated control of surface adaptation by the bacterial flagellum
Curr Opin Microbiol. 2021 Feb 25;61:1-7. doi: 10.1016/j.mib.2021.02.004. Online ahead of print.ABSTRACTMany bacteria can alternate between motile and sessile lifestyles, and wide-ranging sets of environmental stimuli regulate the transition from a free-swimming to a surface-attached state. A transenvelope machine called the flagellum, known primarily for its role in promoting cellular motility, stimulates the motile-sessile transition by detecting contact with solid substrates. Recent work has revealed a striking level of sophistication within the regulatory circuits that link flagellar function to surface colonization. I ...
Source: Current Opinion in Microbiology - February 28, 2021 Category: Microbiology Authors: David M Hershey Source Type: research

The ever-expanding world of bacterial cyclic oligonucleotide second messengers
Curr Opin Microbiol. 2021 Feb 25;60:96-103. doi: 10.1016/j.mib.2021.01.017. Online ahead of print.ABSTRACTCyclic dinucleotide (cdN) second messengers are essential for bacteria to sense and adapt to their environment. These signals were first discovered with the identification of 3'-5', 3'-5' cyclic di-GMP (c-di-GMP) in 1987, a second messenger that is now known to be the linchpin signaling pathway modulating bacterial motility and biofilm formation. In the past 15 years, three more cdNs were uncovered: 3'-5', 3'-5' cyclic di-AMP (c-di-AMP) and 3'-5', 3'-5' cyclic GMP-AMP (3',3' cGAMP) in bacteria and 2'-5', 3'-5' cyclic G...
Source: Current Opinion in Microbiology - February 28, 2021 Category: Microbiology Authors: Soo Hun Yoon Christopher M Waters Source Type: research

Integrated control of surface adaptation by the bacterial flagellum
Curr Opin Microbiol. 2021 Feb 25;61:1-7. doi: 10.1016/j.mib.2021.02.004. Online ahead of print.ABSTRACTMany bacteria can alternate between motile and sessile lifestyles, and wide-ranging sets of environmental stimuli regulate the transition from a free-swimming to a surface-attached state. A transenvelope machine called the flagellum, known primarily for its role in promoting cellular motility, stimulates the motile-sessile transition by detecting contact with solid substrates. Recent work has revealed a striking level of sophistication within the regulatory circuits that link flagellar function to surface colonization. I ...
Source: Current Opinion in Microbiology - February 28, 2021 Category: Microbiology Authors: David M Hershey Source Type: research

The ever-expanding world of bacterial cyclic oligonucleotide second messengers
Curr Opin Microbiol. 2021 Feb 25;60:96-103. doi: 10.1016/j.mib.2021.01.017. Online ahead of print.ABSTRACTCyclic dinucleotide (cdN) second messengers are essential for bacteria to sense and adapt to their environment. These signals were first discovered with the identification of 3'-5', 3'-5' cyclic di-GMP (c-di-GMP) in 1987, a second messenger that is now known to be the linchpin signaling pathway modulating bacterial motility and biofilm formation. In the past 15 years, three more cdNs were uncovered: 3'-5', 3'-5' cyclic di-AMP (c-di-AMP) and 3'-5', 3'-5' cyclic GMP-AMP (3',3' cGAMP) in bacteria and 2'-5', 3'-5' cyclic G...
Source: Current Opinion in Microbiology - February 28, 2021 Category: Microbiology Authors: Soo Hun Yoon Christopher M Waters Source Type: research

New approaches and techniques for bacterial cell wall analysis
Curr Opin Microbiol. 2021 Feb 22;60:88-95. doi: 10.1016/j.mib.2021.01.010. Online ahead of print.ABSTRACTPeptidoglycan (PG) has remained for decades in the spotlight of the never-ending battle against pathogenic bacteria as this essential bacterial structure is one of the most successful targets for antibiotics. Most of our current understanding about the composition, architecture, and dynamics of the PG relies on techniques which have experienced great technological and methodological improvements in the past years. Here we summarize recent advances in these methods with the intention to furnish a valuable resource for bo...
Source: Current Opinion in Microbiology - February 25, 2021 Category: Microbiology Authors: Sara B Hern ández Felipe Cava Source Type: research

New approaches and techniques for bacterial cell wall analysis
Curr Opin Microbiol. 2021 Feb 22;60:88-95. doi: 10.1016/j.mib.2021.01.010. Online ahead of print.ABSTRACTPeptidoglycan (PG) has remained for decades in the spotlight of the never-ending battle against pathogenic bacteria as this essential bacterial structure is one of the most successful targets for antibiotics. Most of our current understanding about the composition, architecture, and dynamics of the PG relies on techniques which have experienced great technological and methodological improvements in the past years. Here we summarize recent advances in these methods with the intention to furnish a valuable resource for bo...
Source: Current Opinion in Microbiology - February 25, 2021 Category: Microbiology Authors: Sara B Hern ández Felipe Cava Source Type: research

New approaches and techniques for bacterial cell wall analysis
Curr Opin Microbiol. 2021 Feb 22;60:88-95. doi: 10.1016/j.mib.2021.01.010. Online ahead of print.ABSTRACTPeptidoglycan (PG) has remained for decades in the spotlight of the never-ending battle against pathogenic bacteria as this essential bacterial structure is one of the most successful targets for antibiotics. Most of our current understanding about the composition, architecture, and dynamics of the PG relies on techniques which have experienced great technological and methodological improvements in the past years. Here we summarize recent advances in these methods with the intention to furnish a valuable resource for bo...
Source: Current Opinion in Microbiology - February 25, 2021 Category: Microbiology Authors: Sara B Hern ández Felipe Cava Source Type: research

Manipulating microcompartment operons to study mechanism and function
Curr Opin Microbiol. 2021 Feb 18;60:66-72. doi: 10.1016/j.mib.2021.01.014. Online ahead of print.ABSTRACTThe gene systems that encode functional bacterial microcompartments (BMCs) are typically comprised of between 10-23 genes, often in a contiguous operon. BMC genes can be studied as whole native operons or as subsets of genes that form structures for specific applications. Recent examples of such studies highlight the flexible modular nature of BMC operons/genes and the options that exist to harness their functions via manipulation at the DNA level. This work also demonstrates the transfer and functional expression of BM...
Source: Current Opinion in Microbiology - February 21, 2021 Category: Microbiology Authors: James W Wilson Source Type: research

Anchoring surface proteins to the bacterial cell wall by sortase enzymes: how it started and what we know now
Curr Opin Microbiol. 2021 Feb 18;60:73-79. doi: 10.1016/j.mib.2021.01.013. Online ahead of print.ABSTRACTIn Gram-positive bacteria, the peptidoglycan serves as a placeholder for surface display of a unique class of monomeric and polymeric proteins, or pili - the precursors of which harbor a cell wall sorting signal with LPXTG motif that is recognized by a conserved transpeptidase enzyme called sortase. Since this original discovery over two decades ago, extensive genetic, biochemical and structural studies have illuminated the basic mechanisms of sortase-mediated cell wall anchoring of surface proteins and pili. We now kno...
Source: Current Opinion in Microbiology - February 21, 2021 Category: Microbiology Authors: Aadil H Bhat Minh Tan Nguyen Asis Das Hung Ton-That Source Type: research

Regulation and function of class A Penicillin-binding proteins
Curr Opin Microbiol. 2021 Feb 18;60:80-87. doi: 10.1016/j.mib.2021.01.008. Online ahead of print.NO ABSTRACTPMID:33611146 | DOI:10.1016/j.mib.2021.01.008 (Source: Current Opinion in Microbiology)
Source: Current Opinion in Microbiology - February 21, 2021 Category: Microbiology Authors: Manuel Pazos Waldemar Vollmer Source Type: research

Manipulating microcompartment operons to study mechanism and function
Curr Opin Microbiol. 2021 Feb 18;60:66-72. doi: 10.1016/j.mib.2021.01.014. Online ahead of print.ABSTRACTThe gene systems that encode functional bacterial microcompartments (BMCs) are typically comprised of between 10-23 genes, often in a contiguous operon. BMC genes can be studied as whole native operons or as subsets of genes that form structures for specific applications. Recent examples of such studies highlight the flexible modular nature of BMC operons/genes and the options that exist to harness their functions via manipulation at the DNA level. This work also demonstrates the transfer and functional expression of BM...
Source: Current Opinion in Microbiology - February 21, 2021 Category: Microbiology Authors: James W Wilson Source Type: research

Anchoring surface proteins to the bacterial cell wall by sortase enzymes: how it started and what we know now
Curr Opin Microbiol. 2021 Feb 18;60:73-79. doi: 10.1016/j.mib.2021.01.013. Online ahead of print.ABSTRACTIn Gram-positive bacteria, the peptidoglycan serves as a placeholder for surface display of a unique class of monomeric and polymeric proteins, or pili - the precursors of which harbor a cell wall sorting signal with LPXTG motif that is recognized by a conserved transpeptidase enzyme called sortase. Since this original discovery over two decades ago, extensive genetic, biochemical and structural studies have illuminated the basic mechanisms of sortase-mediated cell wall anchoring of surface proteins and pili. We now kno...
Source: Current Opinion in Microbiology - February 21, 2021 Category: Microbiology Authors: Aadil H Bhat Minh Tan Nguyen Asis Das Hung Ton-That Source Type: research

Regulation and function of class A Penicillin-binding proteins
Curr Opin Microbiol. 2021 Feb 18;60:80-87. doi: 10.1016/j.mib.2021.01.008. Online ahead of print.NO ABSTRACTPMID:33611146 | DOI:10.1016/j.mib.2021.01.008 (Source: Current Opinion in Microbiology)
Source: Current Opinion in Microbiology - February 21, 2021 Category: Microbiology Authors: Manuel Pazos Waldemar Vollmer Source Type: research