Increasing Upstream Chromatin Long –Range Interactions May Favor Induction of Circular RNAs in LysoPC-Activated Human Aortic Endothelial Cells

We examined the sponging potential of all significantly changed circRNAs using the CircInteractome database (Montefiori et al., 2018), recording two miRNAs with four or more predicted binding sites in a single circRNA transcript, a threshold above which meaningful sponging activity is likely to occur Memczak et al. (2013). Another four significantly changed circRNAs are experimentally shown to sponge miRNAs (Dudekula et al., 2016; Chen et al., 2017; Yan et al., 2017; Wang et al., 2018), for six total circRNAs with miRNA sponging activity including miR125, miR143, miR1272, miR153, miR515-5p, and miR196a-5p (Table 4). In Figure 5A, to demonstrate the proof of principle, we showed how circRNA has_circ_30156 could inhibit type 2 diabetes mellitus (T2DM) by sponging miR143 and preventing miR143 from binding to oxysterol-binding protein-related protein 8 (ORP8) 3′untranslated region (3′UTR). TABLE 4 Table 4. Six circRNAs demonstrate miRNA sponging activities. FIGURE 5 Figure 5. (A) CircRNA has the potential role to inhibit type 2 diabetes mellitus (T2DM)by sponging miRl43 and preventing miRl43 from binding to oxysterol-binding protein-related protein 8 (ORP8) ˆuntranslated region (3′UTR). (B) Upregulated circRNAs sponge miRNAs involved in both pro- and anti-inflammatory pathways. (C) Downregulated circRNAs sponge miRNAs are involved with both pro-inflammatory and anti-inflammatory pathways. (D) One pathway is overlapped in the spo...
Source: Frontiers in Physiology - Category: Physiology Source Type: research