The Development of a Vaccine Against Meningococcus B Using Reverse Vaccinology

The Development of a Vaccine Against Meningococcus B Using Reverse Vaccinology Vega Masignani1, Mariagrazia Pizza1 and E. Richard Moxon2* 1GSK Vaccines, Siena, Italy 2Department of Pediatrics, Oxford University, Oxford, United Kingdom The discovery of vaccine antigens through whole genome sequencing (WGS) contrasts with the classical hypothesis-driven laboratory-based analysis of microbes to identify components to elicit protective immunity. This radical change in scientific direction and action in vaccine research is captured in the term reverse vaccinology. The complete genome sequence of an isolate of Neisseria meningitidis serogroup B (MenB) was systematically analyzed to identify proteins predicted to be secreted or exported to the outer membrane. This identified hundreds of genes coding for potential surface-exposed antigens. These were amplified, cloned in expression vectors and used to immunize mice. Antisera against 350 recombinant antigens were obtained and analyzed in a panel of immunological assays from which 28 were selected as potentially protective based on the -antibody dependent, complement mediated- serum bactericidal activity assay. Testing of these candidate vaccine antigens, using a large globally representative strain collection of Neisseria species isolated from cases of disease and carriage, indicated that no single component would be sufficient to induce broad coverage and that a “universal” vaccine should contain multiple antige...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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Source: Scandinavian Journal of Gastroenterology - Category: Gastroenterology Tags: Scand J Gastroenterol Source Type: research
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Source: Nutrition, Metabolism and Cardiovascular Diseases - Category: Nutrition Source Type: research
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Source: Archives of Medical Science - Category: General Medicine Tags: Arch Med Sci Source Type: research
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Source: Archives of Medical Science - Category: General Medicine Tags: Arch Med Sci Source Type: research
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Source: Archives of Medical Science - Category: General Medicine Tags: Arch Med Sci Source Type: research
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