Endometrial Tumor Microenvironment Alters Human NK Cell Recruitment, and Resident NK Cell Phenotype and Function

This study has been accepted by the national ethics committee (ANSM, Agence Nationale de Sécurité du Médicament, n°130995B-12 and CPP, Comité de Protection des Personnes, n° CPP 13 62). The registration number of the study is ID-RCB: 2013-A00992-43. Our cohort includes patients with endometrial cancer prior to any treatment. The lymph nodes invasion assessment was performed using imaging (MRI, CT scan or PET scan) and, when required, pelvic and/or para-aortic lymphadenectomy. Patient blood samples were collected before the initial surgery. A tumoral sample, along with an adjacent non-invaded endometrial tissue sample (assessed by the pathologist macroscopically), were resected during the initial surgery and before any other treatment. The healthy blood was obtained from French Blood Bank (Etablissement Français du Sang-EFS). Cell Isolation The tissue samples were weighted, and dissociated manually using scalpels in RPMI 1640 (Gibco). The cells were isolated after filtration on 70 μm then 30 μm filters and centrifuged (300 × g for 5 min at 4°C). Tissue supernatants were kept to quantify the cytokines released in the tissue microenvironment. PBMCs were isolated using a Ficoll gradient (Eurobio). Briefly, whole blood was diluted by adding an equal volume of PBS, deposited slowly onto Ficoll media and centrifuged at 800 × g for 30 min at room temperature with no break or acceleratio...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research