Extracellular Vesicles From KSHV-Infected Cells Stimulate Antiviral Immune Response Through Mitochondrial DNA

In this study, we showed that KSHV-infected cells induce interferon-stimulated genes (ISGs) response but not type I interferon in uninfected bystander cells using EVs. mRNA microarray analysis showed that ISGs and IRF-activating genes were prominently activated in EVs from KSHV-infected cells (KSHV EVs)-treated human endothelial cells, which were validated by RT-qPCR and western blot analysis. We also found that this response was not associated with cell death or apoptosis by virus infection. Mechanistically, the cGAS-STING pathway was linked with these KSHV EVs-mediated ISGs expressions, and mitochondrial DNA on the surface of KSHV EVs was one of the causative factors. Besides, KSHV EVs-treated cells showed lower infectivity for KSHV and viral replication activity than mock EVs-treated cells. Our results indicate that EVs from KSHV-infected cells could be an initiating factor for the innate immune response against viral infection, which may be critical to understanding the microenvironment of virus-infected cells. Introduction Cells release vesicles of varying sizes both via the endosomal pathway and by budding from the plasma membrane. These vesicles are referred to by various names, such as exosomes, microvesicles, microparticles, and apoptotic bodies, collectively termed extracellular vesicles (EVs) (1). EVs are a heterogeneous collection of membrane-bound carriers with complex cargoes including proteins, lipids, and nucleic acids, which work as crucial players in...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

Related Links:

In this study we explored the use of a natural compound rubusoside (RUB) as a solubilizer to enhance the solubility of a fluorescence-labeled C6-Ceramide (NBD C6-Ceramide) and to characterize its pharmacokinetics and tissue distribution in an animal model. RUB significantly enhanced the solubility of NBD C6-Ceramide by forming nanomicelles, and efficiently delivered NBD C6-Ceramide in rats by oral and intravenous administration. RUB loaded 1.96% of NBD C6-Ceramide in the nanomicelles and solubilized it to a concentration of 3.6 mg/mL in water. NBD C6-Ceramide in nanomicelles remained stable in aqueous solutions, all...
Source: Prostaglandins and Other Lipid Mediators - Category: Lipidology Source Type: research
Publication date: Available online 10 December 2019Source: Trends in Food Science &TechnologyAuthor(s): Roberto Castro-Muñoz, Grzegorz Boczkaj, Emilia Gontarek, Alfredo Cassano, Vlastimil FílaAbstractBackgroundNowadays, membrane-based technologies (e.g. microfiltration, ultrafiltration, nanofiltration, membrane distillation, and pervaporation) have demonstrated to meet the requirements to be involved in different food and bioproduct processes.Scope and approachSeveral applications have been developed, including either separation, recovery or concentration of bioactive molecules from agro-food products and...
Source: Trends in Food Science and Technology - Category: Food Science Source Type: research
Publication date: Available online 11 December 2019Source: LWTAuthor(s): He Gao, Bo Yang, Catherine Stanton, R. Paul Ross, Hao Zhang, Zhenmin Liu, Haiqin Chen, Wei ChenAbstractThis is the first study to elucidate bifidobacterial conjugated fatty acid and hydroxy fatty acid production as well as its potential in fermented milk. One hundred and fifty-two bifidobacteria belonging to seven species were assayed in this study. The results showed that only Bifidobacterium breve (B. breve), Bifidobacterium longum (B. longum), Bifidobacterium pesudocatenulatum (B. pseudocatenulatum) and Bifidobacterium dentium (B. dentium) generate...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: Available online 10 December 2019Source: Journal of Food Composition and AnalysisAuthor(s): Afsaneh Mohajer, Abbas Norouzian Baghani, Parisa Sadighara, Kiandokht Ghanati, Shahrokh NazmaraAbstractIn this current study, the contents of heavy metals including Pb, As, Cr, Cd, Zn, Cu, and Mn in imported rice bran oil in Iran were investigated with microwave digestion method based on an inductively coupled plasma optical emission spectrometry system (ICP-OES). The concentrations range of Pb, Cr, Cd, As, Zn, and Cu in rice bran oil samples were measured as 0.066 - 0.30, 0.008 - 0.014, 0.005 - 0.01, 0.047- 0.01, ...
Source: Journal of Food Composition and Analysis - Category: Food Science Source Type: research
Publication date: April 2020Source: Food Control, Volume 110Author(s): Sira Sansuk, Paramust Juntarakod, Wimonrat Tongphoothorn, Atchara Sirimungkala, Titikan SomboonAbstractA simple chemo-chronometric method utilizing the Belousov-Zhabotinsky (BZ) oscillating reaction for the efficient detection of ethanol in alcoholic beverages has been developed. This visual detecting strategy only required a smartphone, without implemented instrumental detection systems, to video a unique colorimetric alternating (red-to-blue) feature of the BZ solution system containing 0.50 M bromate, 0.12 M malonic acid, 0.03 M bromid...
Source: Food Control - Category: Food Science Source Type: research
In this study, the effects of the addition of various polysaccharides (konjac gum, gellan gum, and curdlan gum) on the rheological and textural properties of calcium sulfate-induced soy protein isolate gels were investigated. The incorporation of konjac gum and curdlan gum at 0.3 and 0.5% (w/v) concentrations and gellan gum at 0.5% concentration significantly enhanced (P 
Source: Food Chemistry - Category: Food Science Source Type: research
CONCLUSION: Meaningful studies about differential indication for patients with mental disorders are needed. Perspectives for systematic treatment allocation are discussed. PMID: 31822032 [PubMed - as supplied by publisher]
Source: PPmP Psychotherapie Psychosomatik Medizinische Psychologie - Category: Psychiatry & Psychology Tags: Psychother Psychosom Med Psychol Source Type: research
Authors: Wietersheim JV, Knoblauch JD, Rottler E, Weiß H, Hartmann A, Rochlitz P, Völker A, Scheidt CE, Beutel ME, Eckhardt-Henn A, Zeeck A Abstract Several therapeutic treatments like individual psychotherapy, group therapy, creative therapies and talks with the nursing staff are components in an inpatient psychosomatic (psychotherapeutic) treatment or a psychosomatic day hospital treatment. In Germany, these therapies have to be documented by the administration with so called OPS-Codes. These codes are reported to the Institute for Reimbursement in Hospitals (InEK). With the data of the INDDEP-study it...
Source: PPmP Psychotherapie Psychosomatik Medizinische Psychologie - Category: Psychiatry & Psychology Tags: Psychother Psychosom Med Psychol Source Type: research
CONCLUSIONS: The new German short version, the TPV-4R, is an economic, reliable and valid questionnaire to measure spiritual attitudes. PMID: 31822028 [PubMed - as supplied by publisher]
Source: PPmP Psychotherapie Psychosomatik Medizinische Psychologie - Category: Psychiatry & Psychology Tags: Psychother Psychosom Med Psychol Source Type: research
Authors: Volpini L, Mazza C, Mallia L, Guglielmino N, Rossi Berluti F, Fernandes M, Violani C Abstract Background: Reliable Fertility-specific QoL measures can be obtained through the FertiQoL, a questionnaire with six-subscales that consider different core aspects of the person's wellbeing and way of behaving during treatment.Objective: Examine the psychometric properties of all six-subscales of the Italian FertiQoL in a sample of infertile women and explore the effects of the ART treatment phases.Method: 323 women, in three different treatment stages (Diagnostic, Stimulation, Transfer), completed the FertiQoL. Ra...
Source: Journal of Reproductive and Infant Psychology - Category: Psychiatry & Psychology Tags: J Reprod Infant Psychol Source Type: research
More News: Academia | Alcoholism | Allergy & Immunology | Antiviral Therapy | Autoimmune Disease | Biotechnology | Bone Cancers | Cancer | Cancer & Oncology | Chemotherapy | Cold Sores | Cytomegalovirus | Dengue Fever | Doxycycline | Funding | Genetics | Germany Health | Guava | Hepatitis | Hepatitis B | Hepatitis C | Herpes | Insect Bites & Stings | Italy Health | Japan Health | Laboratory Medicine | Learning | Lymphoma | Merck | Mexico Health | Microbiology | Milk | Mitochondrial Disease | Molecular Biology | Nanotechnology | National Institutes of Health (NIH) | Osteosarcoma | PET Scan | Reproduction Medicine | Respiratory Medicine | Sarcomas | Science | Sodium | South Korea Health | Statistics | Stem Cell Therapy | Stem Cells | Students | Study | Universities & Medical Training | USA Health | Vitamin B7