Easy Immunostaining Staining
10X the Signal at a Fraction of the CostTime is money. This is especially true of routine immunostaining assays.We are pleased to introduce you toEZ-HRPTM Polymer Detection. Features include:10-15 times more sensitive than conventional avidin-biotin detection Allows for entire staining procedure to be completed in just under 2 hours Requires less primary antibody (3-4 times less) Does not produce non-specific background staining Can be used on unfixed or chemical fixed (formalin, formaldehyde, alcohol, etc), paraffin-embedded and frozen tissue sections.Comparison of IHC images using EZ-HRP ™ Polymer vs. a c...
Source: Neuromics - July 31, 2018 Category: Neuroscience Tags: HRP-Staining. Immunofluorescence Immunonostaining Source Type: news
Case Shows Biotin Can Interfere With Multiple Endocrine Tests
MONDAY, June 4, 2018 -- Biotin, which is included in many hair, skin, and nail growth over-the-counter products, can affect multiple hormone levels, according to a case study published online May 10 in the Journal of the Endocrine Society. Heather... (Source: Drugs.com - Pharma News)
Source: Drugs.com - Pharma News - June 4, 2018 Category: Pharmaceuticals Source Type: news
Biotin supplements caused misleading test results, almost led to unnecessary procedure
(University of North Carolina Health Care) A new case report led by Maya Styner, M.D., of the University of North Carolina School of Medicine describes how a patient's use of a common over-the-counter biotin supplement caused clinically misleading test results and almost resulted in an unnecessary, invasive medical procedure. (Source: EurekAlert! - Medicine and Health)
Source: EurekAlert! - Medicine and Health - May 18, 2018 Category: International Medicine & Public Health Source Type: news
Vitamin B7 (biotin) is essential for hair, skin and nails – and also supports metabolism, gut health, immunity and more
(Natural News) If you’re having hair and skin problems, try to eat more foods that are rich in vitamin B7 or biotin. Biotin is a B vitamin, and this group of vitamins helps with optimal bodily function. There are many “delicate, intricate pathways” like methylation, or your body’s biochemical superhighway fueled by B vitamins. Methylation... (Source: NaturalNews.com)
Source: NaturalNews.com - March 21, 2018 Category: Consumer Health News Source Type: news
Patient Taking Biotin? Be Sure to Ask if Ordering Tests Patient Taking Biotin? Be Sure to Ask if Ordering Tests
A recent safety report from the FDA warns about this vitamin's possible interference with lab tests.Medscape Internal Medicine (Source: Medscape Internal Medicine Headlines)
Source: Medscape Internal Medicine Headlines - January 4, 2018 Category: Internal Medicine Tags: Internal Medicine Commentary Source Type: news
Medical News Today: All you should know about biotin deficiency
A look at deficiency in biotin, the B vitamin that helps the body convert carbohydrates. Included is detail on how it affects pregnancy and treatment. (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - December 29, 2017 Category: Consumer Health News Tags: Nutrition / Diet Source Type: news
Medical News Today: What you should know about biotin-rich foods
A look at biotin-rich foods that can be added to the diet for a range of health benefits. Included is detail on types of fish, meat, and vegetables. (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - December 4, 2017 Category: Consumer Health News Tags: Nutrition / Diet Source Type: news
FDA Warns Biotin Can Distort Lab Tests
The FDA is warning that high doses of the vitamin B7, or biotin, in dietary supplements can interfere with hundreds of common lab tests—including some relied on by ER doctors to diagnose a heart attack. (Source: WebMD Health)
Source: WebMD Health - November 29, 2017 Category: Consumer Health News Source Type: news
Are Supplements Messing Up Your Blood Work?
Today, the U.S. Food and Drug Administration (FDA) issued a Safety Communication titled, “The FDA Warns that Biotin May Interfere with Laboratory Tests.” The FDA is alerting the public, health care providers and laboratory personnel that biotin can interfere with certain lab tests and cause incorrect lab test results. Some supplements, particularly those labeled for hair, skin, and nail benefits, may have high doses of biotin, which may not be clear from the label. Biotin in patient samples can cause falsely high or falsely low results, depending on the test. Clinical decisions made based on...
Source: MDDI - November 29, 2017 Category: Medical Devices Authors: Amanda Pedersen Tags: IVD Source Type: news
Biotin (Vitamin B7) Supplementation Could Affect Lab Results
The FDA is reporting an increase in adverse events and one death tied to inaccurate lab results due to interference of the supplement biotin (vitamin... (Source: Physician's First Watch current issue)
Source: Physician's First Watch current issue - November 29, 2017 Category: Primary Care Source Type: news
FDA Warns Biotin May Interfere With Lab Tests, Including Troponin FDA Warns Biotin May Interfere With Lab Tests, Including Troponin
Supplementation with high doses of biotin, vitamin B7, may interfere with lab test results, including troponin testing for MI.News Alerts (Source: Medscape Medical News Headlines)
Source: Medscape Medical News Headlines - November 28, 2017 Category: Consumer Health News Tags: Cardiology News Alert Source Type: news
The FDA Warns that Biotin May Interfere with Lab Tests: FDA Safety Communication https://go.usa.gov/xn8YY
The FDA Warns that Biotin May Interfere with Lab Tests: FDA Safety Communication https://go.usa.gov/xn8YY (Source: Food and Drug Adminstration (FDA): CDRHNew)
Source: Food and Drug Adminstration (FDA): CDRHNew - November 28, 2017 Category: Medical Devices Authors: ( at FDADeviceInfo) Source Type: news
Biotin Interference With Routine Clinical Immunoassays Biotin Interference With Routine Clinical Immunoassays
The use of biotin supplements may impact the results of immunoassays used to evaluate endocrine function. Find out what there is to know.Endocrine Practice (Source: Medscape Today Headlines)
Source: Medscape Today Headlines - November 13, 2017 Category: Consumer Health News Tags: Diabetes & Endocrinology Journal Article Source Type: news
Taking Biotin May Make Biotinylated Immunoassay Results Inaccurate Taking Biotin May Make Biotinylated Immunoassay Results Inaccurate
Taking biotin supplements may interfere with immunoassays that use biotinylation in their design, yielding inaccurate test results, a new study suggests.Reuters Health Information (Source: Medscape Pathology Headlines)
Source: Medscape Pathology Headlines - September 27, 2017 Category: Pathology Tags: Pathology & Lab Medicine News Source Type: news
Biotin Supplements May Interfere with Blood Tests (CME/CE)
(MedPage Today) -- Supplements linked to false results in biotinylated assays (Source: MedPage Today Endocrinology)
Source: MedPage Today Endocrinology - September 26, 2017 Category: Endocrinology Source Type: news
Medical News Today: Does biotin for hair growth work? Dosage and side effects
Biotin, also known as vitamin B7, is important for a healthy body. We look at the use of biotin to support hair growth, and the science behind it. (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - September 16, 2017 Category: Consumer Health News Tags: Dermatology Source Type: news
Medical News Today: Seven health benefits of biotin
Biotin is a B-vitamin that has benefits for hair, nails and skin, to name a few. Learn more about the benefits and side effects of biotin in this article. (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - July 31, 2017 Category: Consumer Health News Tags: Complementary Medicine / Alternative Medicine Source Type: news
A Bizarre Case of Graves Disease Due to Vitamin Treatment A Bizarre Case of Graves Disease Due to Vitamin Treatment
This unusual case highlights how biotin intake may influence thyroid function immunoassays, leading to misdiagnosis.Journal of the Endocrine Society (Source: Medscape Today Headlines)
Source: Medscape Today Headlines - July 5, 2017 Category: Consumer Health News Tags: Diabetes & Endocrinology Journal Article Source Type: news
Medical News Today: Can Biotin Help Treat Multiple Sclerosis?
What is biotin, can it help with MS, and how might it be used? Learn about current research on biotin and other possible health benefits of this vitamin. (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - January 14, 2017 Category: Consumer Health News Tags: Multiple Sclerosis Source Type: news
Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains
A series of inhibitors have been designed based on 5′-O-sulfamoyl adenosine (AMS) that display tight binding characteristics towards the inhibition of adenylation (A) domains in nonribosomal peptide synthetases (NRPSs). We recently developed an affinity probe for A domains that could be used to facilitate the specific isolation and identification of NRPS modules. Our synthetic probe, which is a biotinylated variant of l-Phe-AMS (l-Phe-AMS-biotin), selectively targets the A domains in NRPS modules that recognize and convert l-Phe to an aminoacyl adenylate in whole proteomes. In this chapter, we describe the design and...
Source: Springer protocols feed by Protein Science - May 8, 2016 Category: Biochemistry Source Type: news
Engineered monomeric streptavidin
A novel streptavidin variant with improved biotin binding characteristics allows stable monovalent detection of biotinylated targets for imaging applications and can be recombinantly fused to introduce a biotin binding tag, report researchers. (Source: ScienceDaily Headlines)
Source: ScienceDaily Headlines - April 1, 2016 Category: Science Source Type: news
Phosphopeptide Detection with Biotin-Labeled Phos-tag
Protein kinases are widely considered to be invaluable target enzymes for drug discovery and for diagnosing diseases and assessing their prognosis. Effective analytical techniques for measuring the activities of cellular protein kinases are therefore required for studies in the field of phosphoproteomics. We have recently developed a highly sensitive microarray-based technique for tracing the activities of protein kinases. A series of peptides that are specific substrates of various protein kinases are immobilized on a glass slide and subjected to phosphorylation by cell lysates. The resulting phosphorylated forms of the v...
Source: Springer protocols feed by Protein Science - November 19, 2015 Category: Biochemistry Source Type: news
MD1003 (Biotin) encouraging for progressive MS
A phase III trial shows evidence that MD1003 (Biotin) may be a safe and effective way of treating progressive MS. After a year on treatment, 1 in 6 of the treatment group showed a decrease of at least 0.5 on the EDSS measure of disability compared to none in the placebo group. The application process to license the drug is expected to begin by the end of the year. Presented at the American Academy of Neurology (AAN) meeting. Neurology Advisor MedPage Today Progressive forms of MS - A to Z of MS (Source: Multiple Sclerosis Trust)
Source: Multiple Sclerosis Trust - April 23, 2015 Category: Neurology Source Type: news
Biotin (MD1003) study
Pre-publicity for a presentation at AAN conference says that a study of the drug found it was associated with improvements in people with progressive MS. Results will be announced later in the week. MedPage Today MD1003 - Biotin - drugs in development (Source: Multiple Sclerosis Trust)
Source: Multiple Sclerosis Trust - April 16, 2015 Category: Neurology Source Type: news
The Strep-tag System for One-Step Affinity Purification of Proteins from Mammalian Cell Culture
The Strep-tag—or its improved version Strep-tagII—is an eight amino acid sequence that can be easily fused or conjugated to any protein or peptide of interest and that was engineered for high affinity toward streptavidin, which otherwise is widely known as a tight biotin-binding reagent. Especially in combination with immobilized Strep-Tactin, a mutant streptavidin specifically optimized toward the Strep-tagII, this system enables the facile one-step affinity purification of various biomolecules, including oligomeric and even membrane proteins. The Strep-tagII/Strep-Tactin interaction shows exquisite specificit...
Source: Springer protocols feed by Biochemistry - March 9, 2015 Category: Biochemistry Source Type: news
Cell Surface Biotinylation of Receptor Tyrosine Kinases to Investigate Intracellular Trafficking
Cell surface biotinylation is a biochemical approach to covalently bind membrane-impermeable biotin to the extracellular domain of membrane proteins, such as receptor tyrosine kinases (RTKs). Subsequent to ligand incubation periods, activated biotinylated receptors may internalize from the cell surface into early endosomes and then travel through intracellular compartments to either recycle back to the membrane or degrade in lysosomes. The biotin-labeled proteins may be detected through affinity purification with streptavidin agarose resins. This chapter describes methods for cell surface biotinylation to assess RTK traffi...
Source: Springer protocols feed by Protein Science - October 17, 2014 Category: Biochemistry Source Type: news
Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium Membranes Designed for Combination Bacteriotherapy Against Tumors
Nanoparticle technology is an emerging approach to resolve difficult-to-manage internal diseases. It is highly regarded, in particular, for medical use in treatment of cancer due to the innate ability of certain nanoparticles to accumulate in the porous environment of tumors and to be toxic to cancer cells. However, the therapeutic success of nanoparticles is limited by the technical difficulty of fully penetrating and thus attacking the tumor. Additionally, while nanoparticles possess seeming-specificity due to the unique physiological properties of tumors themselves, it is difficult to tailor the delivery of nanoparticle...
Source: Springer protocols feed by Infectious Diseases - September 25, 2014 Category: Infectious Diseases Source Type: news
Genetics Home Reference: biotin-thiamine-responsive basal ganglia disease
http://ghr.nlm.nih.gov/condition/biotin-thiamine-responsive-basal-ganglia-disease (Source: NLM General Announcements)
Source: NLM General Announcements - January 23, 2014 Category: Databases & Libraries Source Type: news
Phage-Displayed Single Domain Antibodies as Recognition Elements
The variable domains of antibodies can be expressed as a fusion with pIII, a minor coat protein of the bateriophage M13, for the generation of phage-display antibody reagents. The phage-display system is routinely used to enrich for recombinant antibodies against a specific target antigen from highly diverse naïve and immune libraries. Often once binders are selected, they are expressed as soluble proteins; however, it can be advantageous to use the phage-displayed antibody fragment as a reagent in binding assays. The repeating subunits of the viral capsid allows for significant signal amplification of binding events ...
Source: Springer protocols feed by Biotechnology - November 20, 2013 Category: Biotechnology Source Type: news
Transcriptomic Analysis of Staphylococcus aureus Using Microarray and Advanced Next-Generation RNA-seq Technologies
The transcriptome has shown tremendous potential for the comprehensive investigation of gene expression profiles and transcriptional levels in comparative biology, the identification of regulatory mechanism of transcriptional regulators, and the evaluation of target gene for developing new chemotherapeutic agents, vaccine, and diagnostic methods. The traditional microarray and advanced next-generation RNA sequencing technologies (RNA-seq) provide powerful and effective tools for the determination of the transcriptome of bacterial cells. In this chapter, we provide a detailed protocol for scientists who want to investigate ...
Source: Springer protocols feed by Microbiology - October 6, 2013 Category: Microbiology Source Type: news
Nutrition 101: Biotin
(HealthCastle.com) Biotin is one of 8 B vitamins. All of the B vitamins are water soluble, meaning that the body does not store them but excretes them in the urine. Recommended Intakes The Dietary Reference Intakes (DRI) for biotin are shown below: read more (Source: HealthCastle.com Nutrition Tips - written by Registered Dietitians)
Source: HealthCastle.com Nutrition Tips - written by Registered Dietitians - September 30, 2013 Category: Nutrition Source Type: news
Proud Distributor of RayBiotech's Antibody Arrays and ELISAs
Pioneer and Market LeaderI am pleased to announce we are a distribution partner for RayBiotech. The solutions we offer include Antibody Arrays and ELISA Kits. With these additions, you now have many options for the either the qualitative, semi-quantitative or quantitative measurement of protein expression. From single antibody pair ELISAs to multiplexed arrays. The arrays enable you to simultaneously analyze up to 80 cytokine, inflammatory response, growth factor or angiogenesis related proteins.In this posting, I focus on our new C-Series Antibody Array Kits. The kits utilize the sandwich immunoassay principle, wherein a ...
Source: Neuromics - August 20, 2013 Category: Neuroscience Tags: sandwich ELISA Cytokine signaling Cytokine Antibody Arrays Inflammatory Response Antibody Arrays Angiogenesis Antibody Arrays. Quantibody Arrays C-Series Antibody Arrays Source Type: news
Potential New Antibiotic Operates In A Completely Different Way
University of Adelaide research has produced a potential new antibiotic which could help in the battle against bacterial resistance to antibiotics. The potential new antibiotic targets a bacterial enzyme critical to metabolic processes. The compound is a protein inhibitor which binds to the enzyme (called biotin protein ligase), stopping its action and interrupting the life cycle of the bacteria... (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - July 5, 2013 Category: Consumer Health News Tags: MRSA / Drug Resistance Source Type: news
Detection of Cell Surface Dopamine Receptors
Dopamine receptors are a class of metabotropic G protein-coupled receptors. Plasma membrane expression is a key determinant of receptor signaling, and one that is regulated both by extra and intracellular cues. Abnormal dopamine receptor signaling is implicated in several neuropsychiatric disorders, including schizophrenia and attention deficit hyperactivity disorder, as well as drug abuse. Here, we describe in detail the application of two complementary applications of protein biotinylation and enzyme-linked immunoabsorbent assay (ELISA) for detecting and quantifying levels of dopamine receptors expressed on the cell surf...
Source: Springer protocols feed by Neuroscience - January 11, 2013 Category: Neuroscience Source Type: news