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Medical News Today: Seven health benefits of biotin
Biotin is a B-vitamin that has benefits for hair, nails and skin, to name a few. Learn more about the benefits and side effects of biotin in this article. (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - July 31, 2017 Category: Consumer Health News Tags: Complementary Medicine / Alternative Medicine Source Type: news

A Bizarre Case of Graves Disease Due to Vitamin Treatment A Bizarre Case of Graves Disease Due to Vitamin Treatment
This unusual case highlights how biotin intake may influence thyroid function immunoassays, leading to misdiagnosis.Journal of the Endocrine Society (Source: Medscape Today Headlines)
Source: Medscape Today Headlines - July 5, 2017 Category: Consumer Health News Tags: Diabetes & Endocrinology Journal Article Source Type: news

Medical News Today: Can Biotin Help Treat Multiple Sclerosis?
What is biotin, can it help with MS, and how might it be used? Learn about current research on biotin and other possible health benefits of this vitamin. (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - January 14, 2017 Category: Consumer Health News Tags: Multiple Sclerosis Source Type: news

Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains
A series of inhibitors have been designed based on 5′-O-sulfamoyl adenosine (AMS) that display tight binding characteristics towards the inhibition of adenylation (A) domains in nonribosomal peptide synthetases (NRPSs). We recently developed an affinity probe for A domains that could be used to facilitate the specific isolation and identification of NRPS modules. Our synthetic probe, which is a biotinylated variant of l-Phe-AMS (l-Phe-AMS-biotin), selectively targets the A domains in NRPS modules that recognize and convert l-Phe to an aminoacyl adenylate in whole proteomes. In this chapter, we describe the design and...
Source: Springer protocols feed by Protein Science - May 8, 2016 Category: Biochemistry Source Type: news

Engineered monomeric streptavidin
A novel streptavidin variant with improved biotin binding characteristics allows stable monovalent detection of biotinylated targets for imaging applications and can be recombinantly fused to introduce a biotin binding tag, report researchers. (Source: ScienceDaily Headlines)
Source: ScienceDaily Headlines - April 1, 2016 Category: Science Source Type: news

Phosphopeptide Detection with Biotin-Labeled Phos-tag
Protein kinases are widely considered to be invaluable target enzymes for drug discovery and for diagnosing diseases and assessing their prognosis. Effective analytical techniques for measuring the activities of cellular protein kinases are therefore required for studies in the field of phosphoproteomics. We have recently developed a highly sensitive microarray-based technique for tracing the activities of protein kinases. A series of peptides that are specific substrates of various protein kinases are immobilized on a glass slide and subjected to phosphorylation by cell lysates. The resulting phosphorylated forms of the v...
Source: Springer protocols feed by Protein Science - November 19, 2015 Category: Biochemistry Source Type: news

MD1003 (Biotin) encouraging for progressive MS
A phase III trial shows evidence that MD1003 (Biotin) may be a safe and effective way of treating progressive MS. After a year on treatment, 1 in 6 of the treatment group showed a decrease of at least 0.5 on the EDSS measure of disability compared to none in the placebo group. The application process to license the drug is expected to begin by the end of the year. Presented at the American Academy of Neurology (AAN) meeting. Neurology Advisor MedPage Today Progressive forms of MS - A to Z of MS (Source: Multiple Sclerosis Trust)
Source: Multiple Sclerosis Trust - April 23, 2015 Category: Neurology Source Type: news

Biotin (MD1003) study
Pre-publicity for a presentation at AAN conference says that a study of the drug found it was associated with improvements in people with progressive MS. Results will be announced later in the week. MedPage Today MD1003 - Biotin - drugs in development (Source: Multiple Sclerosis Trust)
Source: Multiple Sclerosis Trust - April 16, 2015 Category: Neurology Source Type: news

The Strep-tag System for One-Step Affinity Purification of Proteins from Mammalian Cell Culture
The Strep-tag—or its improved version Strep-tagII—is an eight amino acid sequence that can be easily fused or conjugated to any protein or peptide of interest and that was engineered for high affinity toward streptavidin, which otherwise is widely known as a tight biotin-binding reagent. Especially in combination with immobilized Strep-Tactin, a mutant streptavidin specifically optimized toward the Strep-tagII, this system enables the facile one-step affinity purification of various biomolecules, including oligomeric and even membrane proteins. The Strep-tagII/Strep-Tactin interaction shows exquisite specificit...
Source: Springer protocols feed by Biochemistry - March 9, 2015 Category: Biochemistry Source Type: news

Cell Surface Biotinylation of Receptor Tyrosine Kinases to Investigate Intracellular Trafficking
Cell surface biotinylation is a biochemical approach to covalently bind membrane-impermeable biotin to the extracellular domain of membrane proteins, such as receptor tyrosine kinases (RTKs). Subsequent to ligand incubation periods, activated biotinylated receptors may internalize from the cell surface into early endosomes and then travel through intracellular compartments to either recycle back to the membrane or degrade in lysosomes. The biotin-labeled proteins may be detected through affinity purification with streptavidin agarose resins. This chapter describes methods for cell surface biotinylation to assess RTK traffi...
Source: Springer protocols feed by Protein Science - October 17, 2014 Category: Biochemistry Source Type: news

Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium Membranes Designed for Combination Bacteriotherapy Against Tumors
Nanoparticle technology is an emerging approach to resolve difficult-to-manage internal diseases. It is highly regarded, in particular, for medical use in treatment of cancer due to the innate ability of certain nanoparticles to accumulate in the porous environment of tumors and to be toxic to cancer cells. However, the therapeutic success of nanoparticles is limited by the technical difficulty of fully penetrating and thus attacking the tumor. Additionally, while nanoparticles possess seeming-specificity due to the unique physiological properties of tumors themselves, it is difficult to tailor the delivery of nanoparticle...
Source: Springer protocols feed by Infectious Diseases - September 25, 2014 Category: Infectious Diseases Source Type: news

Genetics Home Reference: biotin-thiamine-responsive basal ganglia disease
http://ghr.nlm.nih.gov/condition/biotin-thiamine-responsive-basal-ganglia-disease (Source: NLM General Announcements)
Source: NLM General Announcements - January 23, 2014 Category: Databases & Libraries Source Type: news

Phage-Displayed Single Domain Antibodies as Recognition Elements
The variable domains of antibodies can be expressed as a fusion with pIII, a minor coat protein of the bateriophage M13, for the generation of phage-display antibody reagents. The phage-display system is routinely used to enrich for recombinant antibodies against a specific target antigen from highly diverse naïve and immune libraries. Often once binders are selected, they are expressed as soluble proteins; however, it can be advantageous to use the phage-displayed antibody fragment as a reagent in binding assays. The repeating subunits of the viral capsid allows for significant signal amplification of binding events ...
Source: Springer protocols feed by Biotechnology - November 20, 2013 Category: Biotechnology Source Type: news

Transcriptomic Analysis of Staphylococcus aureus Using Microarray and Advanced Next-Generation RNA-seq Technologies
The transcriptome has shown tremendous potential for the comprehensive investigation of gene expression profiles and transcriptional levels in comparative biology, the identification of regulatory mechanism of transcriptional regulators, and the evaluation of target gene for developing new chemotherapeutic agents, vaccine, and diagnostic methods. The traditional microarray and advanced next-generation RNA sequencing technologies (RNA-seq) provide powerful and effective tools for the determination of the transcriptome of bacterial cells. In this chapter, we provide a detailed protocol for scientists who want to investigate ...
Source: Springer protocols feed by Microbiology - October 6, 2013 Category: Microbiology Source Type: news

Nutrition 101: Biotin
(HealthCastle.com) Biotin is one of 8 B vitamins. All of the B vitamins are water soluble, meaning that the body does not store them but excretes them in the urine.  Recommended Intakes The Dietary Reference Intakes (DRI) for biotin are shown below: read more (Source: HealthCastle.com Nutrition Tips - written by Registered Dietitians)
Source: HealthCastle.com Nutrition Tips - written by Registered Dietitians - September 30, 2013 Category: Nutrition Source Type: news

Proud Distributor of RayBiotech's Antibody Arrays and ELISAs
Pioneer and Market LeaderI am pleased to announce we are a distribution partner for RayBiotech. The solutions we offer include Antibody Arrays and ELISA Kits. With these additions, you now have many options for the either the qualitative, semi-quantitative or quantitative measurement of protein expression. From single antibody pair ELISAs to multiplexed arrays. The arrays enable you to simultaneously analyze up to 80 cytokine, inflammatory response, growth factor or angiogenesis related proteins.In this posting, I focus on our new C-Series Antibody Array Kits. The kits utilize the sandwich immunoassay principle, wherein a ...
Source: Neuromics - August 20, 2013 Category: Neuroscience Tags: sandwich ELISA Cytokine signaling Cytokine Antibody Arrays Inflammatory Response Antibody Arrays Angiogenesis Antibody Arrays. Quantibody Arrays C-Series Antibody Arrays Source Type: news

Potential New Antibiotic Operates In A Completely Different Way
University of Adelaide research has produced a potential new antibiotic which could help in the battle against bacterial resistance to antibiotics. The potential new antibiotic targets a bacterial enzyme critical to metabolic processes. The compound is a protein inhibitor which binds to the enzyme (called biotin protein ligase), stopping its action and interrupting the life cycle of the bacteria... (Source: Health News from Medical News Today)
Source: Health News from Medical News Today - July 5, 2013 Category: Consumer Health News Tags: MRSA / Drug Resistance Source Type: news

Detection of Cell Surface Dopamine Receptors
Dopamine receptors are a class of metabotropic G protein-coupled receptors. Plasma membrane expression is a key determinant of receptor signaling, and one that is regulated both by extra and intracellular cues. Abnormal dopamine receptor signaling is implicated in several neuropsychiatric disorders, including schizophrenia and attention deficit hyperactivity disorder, as well as drug abuse. Here, we describe in detail the application of two complementary applications of protein biotinylation and enzyme-linked immunoabsorbent assay (ELISA) for detecting and quantifying levels of dopamine receptors expressed on the cell surf...
Source: Springer protocols feed by Neuroscience - January 11, 2013 Category: Neuroscience Source Type: news