Query: siRNA

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Hepatitis C Virus Entry into Macrophages/Monocytes Mainly Depends on the Phagocytosis of Macrophages
ConclusionsHCV entry into macrophages mainly depends on phagocytosis of macrophages.
Source: Digestive Diseases and Sciences - December 10, 2018 Category: Gastroenterology Source Type: research

Inhibition of HOXB7 suppresses p27-mediated acute lymphoblastic leukemia by regulating basic fibroblast growth factor and ERK1/2
In this study, we explored the molecular mechanism of HOXB7 in cell viability and cell cycle in ALL cell lines.Materials and methodsPeripheral blood lymphocytes was isolated by Isopycnic Ficoll-Hypaque solution; Relative mRNA expression of HOXB7 was measured by RT-qPCR; Relative protein expressions of HOXB7, p27, bFGF, pERK1/2 were tested by Western blot assay; Cell viability was tested by MTT; Cell proliferation was detected by BrdU assay; 2.8. Cell cycle was analyzed by flow cytometry.Key findingsHOXB7 was significantly elevated in peripheral blood lymphocytes of patients with ALL. HOXB7 was inhibited by HOXB7 siRNA tran...
Source: Life Sciences - December 9, 2018 Category: Biology Source Type: research

Tropomyosin Tpm 2.1 loss induces glioblastoma spreading in soft brain-like environments
ConclusionsWe propose that Tpm 2.1 down-regulation facilitates GBM colonisation of the soft brain environment. This specialisation of the GBM actin cytoskeleton organisation that is highly suited to the soft brain-like environment may provide novel therapeutic targets for arresting GBM invasion.
Source: Journal of Neuro-Oncology - December 9, 2018 Category: Cancer & Oncology Source Type: research

High-Throughput Sequencing Strategy for miR-146b-regulated circRNA Expression in Hepatic Stellate Cells.
CONCLUSIONS miR-146b could regulate the expression of circRNAs in HSCs, which might take part in the formation and development of hepatic fibrosis. PMID: 30504757 [PubMed - in process]
Source: Medical Science Monitor - December 8, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Recent Advances in Oligonucleotide-Based Therapy for Transthyretin Amyloidosis: Clinical Impact and Future Prospects.
Abstract Transthyretin (TTR) amyloidosis, also known as transthyretin-related familial amyloidotic polyneuropathy (ATTR-FAP), is a fatal hereditary systemic amyloidosis caused by mutant forms of TTR. Although conventional treatments for ATTR-FAP, such as liver transplantation (LT) and TTR tetramer stabilizer, reportedly halt the progression of clinical manifestation, these therapies have several limitations. Oligonucleotide-based therapy, e.g. small interfering RNA (siRNA)- and antisense oligonucleotides (ASOs)-based therapy, hold enormous potential for the treatment of intractable diseases such as ATTR-FAP, by sp...
Source: Biological and Pharmaceutical Bulletin - December 8, 2018 Category: Drugs & Pharmacology Authors: Hayashi Y, Jono H Tags: Biol Pharm Bull Source Type: research

Interaction of ovarian steroidogenesis and clock gene expression modulated by bone morphogenetic protein-7 in human granulosa cells.
Authors: Nagao S, Iwata N, Soejima Y, Takiguchi T, Aokage T, Kozato Y, Nakano Y, Nada T, Hasegawa T, Otsuka F Abstract A functional link between clock gene expression and ovarian steroidogenesis was studied using human granulosa KGN cells. Similarities between changes in the mRNA and protein expression levels of Bmal1 and Clock and those of Per2 and Cry1 were found in KGN cells after treatment with forskolin. Among the interrelationships between the expression levels of clock and steroidogenic factors, Clock mRNA had a strongly positive correlation with P450arom and a negative correlation with 3βHSD. Knockdown of ...
Source: Endocrine Journal - December 8, 2018 Category: Endocrinology Tags: Endocr J Source Type: research

Cancers, Vol. 10, Pages 499: Mitochondrial VDAC1 Silencing Leads to Metabolic Rewiring and the Reprogramming of Tumour Cells into Advanced Differentiated States
an-Barmatz Oncogenic properties, along with the metabolic reprogramming necessary for tumour growth and motility, are acquired by cancer cells. Thus, tumour metabolism is becoming a target for cancer therapy. Here, cancer cell metabolism was tackled by silencing the expression of voltage-dependent anion channel 1 (VDAC1), a mitochondrial protein that controls cell energy, as well as metabolic and survival pathways and that is often over-expressed in many cancers. We demonstrated that silencing VDAC1 expression using human-specific siRNA (si-hVDAC1) inhibited cancer cell growth, both in vitro and in mouse xenograft mode...
Source: Cancers - December 8, 2018 Category: Cancer & Oncology Authors: Arif Paul Krelin Shteinfer-Kuzmine Shoshan-Barmatz Tags: Article Source Type: research

Nuclear RXRα and RXRβ receptors exert distinct and opposite effects on RA-mediated neuroblastoma differentiation
Publication date: Available online 7 December 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Carolina Saibro Girardi, Diana Carolina Rostirolla, Fernanda Janini Mota Lini, Pedro Ozorio Brum, Jeferson Delgado, Camila Tiefensee Ribeiro, Alexsander Alves Teixeira, Daniel Oppermann Peixoto, Luana Heimfarth, Alice Kunzler, José Cláudio Fonseca Moreira, Daniel Pens GelainAbstractRetinoic acid (RA) promotes differentiation in multiple neurogenic cell types by promoting gene reprogramming through retinoid receptors and also by inducing cytosolic signaling events. The nuclear RXR receptors are...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 7, 2018 Category: Molecular Biology Source Type: research

Molecular cloning and functional characterization of a short peptidoglycan recognition protein from triangle-shell pearl mussel (Hyriopsis cumingii)
In this study, we cloned and functionally analyzed a short PGRP (HcPGRP2) from the triangle-shell pearl mussel Hyriopsis cumingii. The full-length cDNA sequence of HcPGRP2 gene was 1185 bp containing an open reading frame of 882 bp encoding a 293 amino acid protein. HcPGRP2 was predicted to have two SH3b domains and a conserved C-terminal PGRP domain. Quantitative real-time RT-PCR showed that HcPGRP2 was expressed in all examined tissues and its expression was induced most significantly by Staphylococcus aureus and Vibrio parahaemolyticus in the hepatopancreas and gills. RNA interference by siRNA results revealed that HcPG...
Source: Fish and Shellfish Immunology - December 7, 2018 Category: Biology Source Type: research

The transcription factor STAT6 plays a critical role in promoting beta cell viability and is depleted in islets of individuals with type 1 diabetes
Conclusions/interpretationSelective loss of STAT6 may contribute to beta cell demise during the progression of type 1 diabetes.
Source: Diabetologia - December 7, 2018 Category: Endocrinology Source Type: research

Naringenin targets on astroglial Nrf2 to support dopaminergic neurons
Publication date: Available online 6 December 2018Source: Pharmacological ResearchAuthor(s): Guo-Qing Wang, Bei Zhang, Xue-Mei He, Dai-Di Li, Jing-Shan Shi, Feng ZhangAbstractAstroglia serve as a critical role in metabolic and neurotrophic support to neurons. The loss of astroglia-derived neurotrophic effects could be a primary contributor to Parkinson’s disease (PD). Thus, understanding astroglia functions is an important strategy for enhancing neuronal survival. Nuclear factor erythroid 2-related factor 2 (Nrf2) plays a key role in neuronal resistance to oxidative stress and glutamate-induced excitotoxicity. Balancing ...
Source: Pharmacological Research - December 7, 2018 Category: Drugs & Pharmacology Source Type: research

Mammalian STE20-Like Kinase 1 Deletion Alleviates Renal Ischaemia-Reperfusion Injury via Modulating Mitophagy and the AMPK-YAP Signalling Pathway
Conclusion: Overall, our results demonstrate that the pathogenesis of renal I/R injury is closely associated with an increase in Mst1 expression and the inactive AMPK-YAP-OPA1 signalling pathway. Based on this, strategies to repress Mst1 expression and activate mitophagy could serve as therapeutic targets to treat kidney ischaemia-reperfusion injury.Cell Physiol Biochem 2018;51:2359 –2376
Source: Cellular Physiology and Biochemistry - December 7, 2018 Category: Cytology Source Type: research

GSE123410 RNA-seq data for non-targeting siRNA and CDK11 siRNA transfection in SHhES8 cells
Contributors : Jianyi Ding ; Ying JinSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe found that CDK11 KD in hESCs caused hESC differentiation. To get the detailed mechanism why its KD caused differentiation, we applied RNA-seq analysis, aiming to find out some clues about the mechanism. Firstly, we obtained the differentially expressed genes (DEGs) from NC and CDK11 KD hESC cells, replicated NC and 2 sets of CDK11 siRNA were transfected into SHhES8 cells for 4 days. Later, we analyzed the data and used GO analysis to enrich GO terms which might be helpful for our research.
Source: GEO: Gene Expression Omnibus - December 7, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Sonodynamic therapy-induced foam cells apoptosis activates the phagocytic PPAR γ -LXR α -ABCA1/ABCG1 pathway and promotes cholesterol efflux in advanced plaque
Conclusions: Activation of PPARγ-LXRα-ABCA1/ABCG1 pathway induced by ALA-SDT treatment engages a virtuous cycle that enhances efferocytosis, cholesterol efflux and anti-inflammatory reactions in advanced plaque in vivo and in phagocytes in vitro.
Source: Theranostics - December 7, 2018 Category: Molecular Biology Authors: Huan Wang, Yang Yang, Xin Sun, Fang Tian, Shuyuan Guo, Wei Wang, Zhen Tian, Hong Jin, Zhiguo Zhang, Ye Tian Tags: Research Paper Source Type: research

Mesenchymal glioblastoma constitutes a major ceRNA signature in the TGF- β pathway
Conclusions: This study suggests that the micNETs may have translational significance in the diagnosis of mesenchymal GBM and may be novel therapeutic targets.
Source: Theranostics - December 7, 2018 Category: Molecular Biology Authors: Qixue Wang, Jinquan Cai, Chuan Fang, Chao Yang, Junhu Zhou, Yanli Tan, Yunfei Wang, Yansheng Li, Xiangqi Meng, Kai Zhao, Kaikai Yi, Sijing Zhang, Jianning Zhang, Chuanlu Jiang, Jing Zhang, Chunsheng Kang Tags: Research Paper Source Type: research

ATP-activated decrosslinking and charge-reversal vectors for siRNA delivery and cancer therapy
Conclusion: These results indicated that the ATP-triggered disassembly and charge reversal strategy provided a new way for developing stimuli-responsive siRNA carriers and showed potential for nucleic acid delivery in the treatment of cancer.
Source: Theranostics - December 7, 2018 Category: Molecular Biology Authors: Zhanwei Zhou, Qingyan Zhang, Minghua Zhang, Huipeng Li, Gang Chen, Chenggen Qian, David Oupicky, Minjie Sun Tags: Research Paper Source Type: research

Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19
Conclusion: CAFs promote the stemness and chemoresistance of CRC by transferring exosomal H19. H19 activated the β-catenin pathway via acting as a competing endogenous RNA sponge for miR-141, while miR-141 inhibited the stemness of CRC cells. Our findings indicate that H19 expressed by CAFs of the colorectal tumor stroma contributes to tumor development and chemoresistance.
Source: Theranostics - December 7, 2018 Category: Molecular Biology Authors: Jing Ren, Liang Ding, Dongya Zhang, Guoping Shi, Qianyun Xu, Sunan Shen, Yaping Wang, Tingting Wang, Yayi Hou Tags: Research Paper Source Type: research

CDC20 regulates cardiac hypertrophy via targeting LC3-dependent autophagy
Conclusions: Our study reveals a novel cardiac hypertrophy regulatory mechanism that involves CDC20, LC3 and autophagy, and suggests that CDC20 could be a new therapeutic target for patients with hypertrophic heart diseases.
Source: Theranostics - December 7, 2018 Category: Molecular Biology Authors: Yun-Peng Xie, Song Lai, Qiu-Yue Lin, Xin Xie, Jia-Wei Liao, Hong-Xia Wang, Cui Tian, Hui-Hua Li Tags: Research Paper Source Type: research

BPI-9016M, a c-Met inhibitor, suppresses tumor cell growth, migration and invasion of lung adenocarcinoma via miR203-DKK1
Conclusion: Our data indicated that BPI-9016M is an effective agent against lung adenocarcinoma, particularly in tumors with c-Met activation, and likely functions through upregulation of miR203 leading to reduced DKK1 expression.
Source: Theranostics - December 7, 2018 Category: Molecular Biology Authors: Panpan Zhang, Shaolei Li, Chao Lv, Jiahui Si, Ying Xiong, Lieming Ding, Yuanyuan Ma, Yue Yang Tags: Research Paper Source Type: research

RE1-silencing transcription factor controls the acute-to-chronic neuropathic pain transition and Chrm2 receptor gene expression in primary sensory neurons Neurobiology
Neuropathic pain is associated with persistent changes in gene expression in primary sensory neurons, but the underlying epigenetic mechanisms that cause these changes remain unclear. The muscarinic cholinergic receptors (mAChRs), particularly the M2 subtype (encoded by the cholinergic receptor muscarinic 2 (Chrm2) gene), are critically involved in the regulation of spinal nociceptive transmission. However, little is known about how Chrm2 expression is transcriptionally regulated. Here we show that nerve injury persistently increased the expression of RE1-silencing transcription factor (REST, also known as neuron-restricti...
Source: Journal of Biological Chemistry - December 7, 2018 Category: Chemistry Authors: Jixiang Zhang, Shao-Rui Chen, Hong Chen, Hui-Lin Pan Tags: Molecular Bases of Disease Source Type: research

Leucine regulates slow ‐twitch muscle fibers expression and mitochondrial function by Sirt1/AMPK signaling in porcine skeletal muscle satellite cells
In this study, porcine skeletal muscle satellite cells cultured in differentiation medium were treated with 2 mM leucine for 3 days. Sirt1 inhibitor EX52 7, AMPK inhibitor compound C, and AMPKα1 siRNA were used to examine its underlying mechanism. Here we showed that leucine increased slow‐twitch muscle fibers and mitochondrial function‐related gene expression, as well as increased succinic dehydrogenase (SDH) and malate dehydrogenase (MDH) acti vities. Moreover, leucine increased the protein levels of Sirt1 and phospho‐AMPK. We also found that AMPKα1 siRNA, AMPK inhibitor compound C, or Sirt1 inhibitor EX527 atte...
Source: Animal Science Journal - December 6, 2018 Category: Zoology Authors: Xiaoling Chen, Lu Xiang, Gang Jia, Guangmang Liu, Hua Zhao, Zhiqing Huang Tags: ORIGINAL ARTICLE Source Type: research

First identification of PODXL nonsense mutations in autosomal dominant focal segmental glomerulosclerosis
In this study, we report the identification of a heterozygous nonsense PODXL mutations (Arg326X) in a Chinese pedigree featured by proteinuria and renal insufficiency with AD inheritance by whole exome sequencing (WES). Total mRNA and PODXL protein abundance were decreased in available peripheral blood cell samples of two affected patients undergoing hemodialysis, compared to those in healthy controls and hemodialysis controls without PODXL mutation. We identified another novel PODXL heterozygous nonsense mutation (c.C1133G; p.Ser378X) in a British-Indian pedigree of AD-FSGS by WES. In vitro study showed that, human embryo...
Source: Clinical Science - December 6, 2018 Category: Biomedical Science Authors: Lin, F.-J., Yao, L., Hu, X.-Q., Bian, F., Ji, G., Jiang, G.-R., Gale, D. P., Ren, H.-Q. Tags: PublishAheadOfPrint Source Type: research

Viruses, Vol. 10, Pages 694: Antiviral RNA Interference Activity in Cells of the Predatory Mosquito, Toxorhynchites amboinensis
In this study, we investigated the presence of an RNAi response in Toxorhynchites amboinensis, which is a non-blood feeding species proposed as a biological control agent against pest mosquitoes. Using a derived cell line (TRA-171), we demonstrate that these mosquitoes possess a functional RNAi response that is active against a mosquito-borne alphavirus, Semliki Forest virus. As observed in vector mosquito species, small RNAs are produced that target viral sequences. The size and characteristics of these small RNAs indicate that both the siRNA and piRNA pathways are induced in response to infection. Taken together, this da...
Source: Viruses - December 6, 2018 Category: Virology Authors: Claire L. Donald Margus Varjak Eric Roberto Guimar ães Rocha Aguiar Jo ão T. Marques Vattipally B. Sreenu Esther Schnettler Alain Kohl Tags: Article Source Type: research

Highly Active Enzymes Immobilized in Large Pore Colloidal Mesoporous Silica Nanoparticles
New J. Chem., 2018, Accepted Manuscript DOI: 10.1039/C8NJ04585B, PaperDoroth ée Gößl, Helena Singer, Hsin-Yi Chiu, Alexandra Schmidt, Martina Lichtnecker, Hanna Engelke, Thomas Bein Various bio-applications of mesoporous materials (e.g., the immobilization of enzymes or the delivery of biomolecules such as siRNA) require large pores for the successful adsorption of the rather large molecules... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - New J. Chem. latest articles - December 5, 2018 Category: Chemistry Authors: Doroth ée Gößl Source Type: research

ATP-responsive low-molecular-weight polyethylenimine-based supramolecular assembly via host-guest interaction for gene delivery.
Abstract In this work, we report on an ATP-responsive low-molecular-weight polyethylenimine (LMW-PEI)-based supramolecular assembly. It formed via host-guest interaction between PEI (MW=1.8 kDa)-α-cyclodextrin (α-CD) conjugates and PEI1.8k-phenylboronic acid (PBA) conjugates. The host-guest interaction between PEI1.8k-α-CD and PEI1.8k-PBA was confirmed by the 2D-NOESY chromatogram experiment and competition test. The ATP-responsive property of the supramolecular assembly was evaluated by a series of ATP-triggered degradation and siRNA release studies in terms of fluorescence resonance energy transfer, agarose g...
Source: Biomacromolecules - December 5, 2018 Category: Biochemistry Authors: Jiang C, Qi Z, Jia H, Huang Y, Wang Y, Zhang W, Wu Z, Yang H, Liu J Tags: Biomacromolecules Source Type: research

Honokiol induces apoptosis of lung squamous cell carcinoma by targeting FGF2 ‐FGFR1 autocrine loop
In conclusion, honokiol induced cell apoptosis in lung SCC by targeting the FGF2‐FGFR1 autocrine loop.
Source: Cancer Medicine - December 5, 2018 Category: Cancer & Oncology Authors: Mengyuan Cen, Yinan Yao, Luyun Cui, Guangdie Yang, Guohua Lu, Liangjie Fang, Zhang Bao, Jianying Zhou Tags: ORIGINAL RESEARCH Source Type: research

Macrophage stimulating protein preserves blood brain barrier integrity after intracerebral hemorrhage through recepteur d'origine nantais dependent GAB1/Src/ β‐catenin pathway activation in a mouse model
This study aimed to evaluate the role of MSP and RON activated signaling pathway in preserving BBB integrity after collagenase‐induced ICH. ICH mice received recombinant human MSP (rhMSP) or rhMSP combined with siRNA knockdown of RON or GAB1. rhMSP was administered by intranasal route 1 h after ICH. Brain edema, neurobehavior, BBB tight junction protein exp ression, and BBB permeability were evaluated. The expression of endogenous MSP and p‐RON was decreased after ICH. Exogenous rhMSP administration reduced brain edema, neurological deficits, BBB permeability, and increased the expression of tight junction proteins in...
Source: Journal of Neurochemistry - December 5, 2018 Category: Neuroscience Authors: Tai Lu, Zhong Wang, Sherchan Prativa, Yang Xu, Tian Wang, Yiting Zhang, Lingyan Yu, Ningbo Xu, Jiping Tang, Wanchun You, Gang Chen, John H. Zhang Tags: Original Article Source Type: research

Molecules, Vol. 23, Pages 3209: Aquaporin 11-Dependent Inhibition of Proliferation by Deuterium Oxide in Activated Hepatic Stellate Cells
Pyo Kim Deuterium oxide (D2O) has been reported to be active toward various in vitro cell lines in combination with phytochemicals. Our objective was to describe, for the first time, the effect of D2O on the proliferation of hepatic stellate cells (HSCs). After D2O treatment, the p53-cyclin-dependent kinase (CDK) pathway was stimulated, leading to inhibition of the proliferation of HSCs and an increase in the [ATP]/[ADP] ratio. We also evaluated the role of aquaporin (AQP) 11 in activated HSCs. We found that D2O treatment decreased AQP11 expression levels. Of note, AQP11 levels elevated by a genetic approach counteract...
Source: Molecules - December 5, 2018 Category: Chemistry Authors: Phil Jun Lee Hye-Jin Park Namki Cho Hong Pyo Kim Tags: Article Source Type: research

Simvastatin Protects Dopaminergic Neurons Against MPP < sup > + < /sup > -Induced Oxidative Stress and Regulates the Endogenous Anti-Oxidant System Through ERK
Conclusion: Our results provide strong evidence that ERK1/2-mediated modulation of the anti-oxidant system after simvastatin treatment may partially explain the anti-oxidant activity in experimental parkinsonian models. These findings contribute to a better understanding of the critical roles of simvastatin via the ERK1/2-mediated modulation of the anti-oxidant system, which may be relevant for treating PD.Cell Physiol Biochem 2018;51:1957 –1968
Source: Cellular Physiology and Biochemistry - December 4, 2018 Category: Cytology Source Type: research

Extracellular FABP4 uptake by endothelial cells is dependent on cytokeratin 1 expression
ConclusionsWe demonstrated that CK1 facilitates eFABP4 cellular uptake in endothelial cells. Therefore, the CK1-targeted inhibition of exogenous FABP4 cellular uptake might be a potential therapeutic strategy to protect endothelial cells against FABP4-induced activation of inflammation and oxidative stress.
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - December 4, 2018 Category: Lipidology Source Type: research

Cai's Neiyi Prescription promotes apoptosis and inhibits inflammation in endometrial stromal cells with endometriosis through inhibiting USP10
AbstractTo observe the effect of Cai's Neiyi Prescription (CNYP) on the apoptosis and inflammation in endometrial stromal cells with endometriosis (EM) bothin vivo andin vitro, EM model rats and endometrial stromal cells were treated with CNYP and the level of USP10, p ‐ERK1/2, ERK1/2, and apoptosis‐related protein as well as the levels of proinflammatory factors were measured by Western blotting and ELISA, respectively. Rats with surgically induced EM showed increased USP10 expression and ERK/2 activation. Intragastric administration of CNYP granule significa ntly inhibited EM‐induced ERK1/2 activation and expressio...
Source: Biotechnology and Applied Biochemistry - December 4, 2018 Category: Biochemistry Authors: Shuangdi Li, Yaping Zhu, Tingting Zhang, Yuanyuan Hang, Qiong Chen, Yuli Jin Tags: Original Article Source Type: research

TGF- β1 Induces Human Tenon's Fibroblasts Fibrosis via miR-200b and Its Suppression of PTEN Signaling.
CONCLUSION: TGF-β1-mediated miR-200b induces HTFs fibrosis through suppressing PTEN signaling pathway. PMID: 30512998 [PubMed - as supplied by publisher]
Source: Current Eye Research - December 4, 2018 Category: Opthalmology Authors: Tong J, Chen F, Du W, Zhu J, Xie Z Tags: Curr Eye Res Source Type: research

Effects of JUN and NFE2L2 knockdown on oxidative status and NFE2L2/AP-1 targets expression in HeLa cells in basal conditions and upon sub-lethal hydrogen peroxide treatment.
Abstract Although NFE2L2 transcription factor is considered to make the most significant contribution to the NFE2L2/AP-1-pathway-dependent antioxidants regulation in the human cell, AP-1 has the potential to provide significant backup and even play an equal role in the cell. Considering this, the present study is focused on revealing how JUN, an AP-1 component, and NFE2L2 contribute to regulation of four target genes containing AREs with embedded TREs-SQSTM1, FTH1, HMOX1 and CBR3 and to cellular oxidative status in general in basal conditions and under pro-oxidative influence. NFE2L2 and JUN were down-regulated in...
Source: Molecular Biology Reports - December 4, 2018 Category: Molecular Biology Authors: Belanova AA, Chmykhalo VK, Makarenko MS, Lyangasova OV, Belousova MM, Aleksandrova AA, Zolotukhin PV Tags: Mol Biol Rep Source Type: research

EDG5 plays an important role in induction and maintenance of pluripotency
Graphical abstract summarizing the main findings generated from this study. AbstractDirect reprogramming of human somatic cells toward induced pluripotent stem cells holds great promise for regenerative medicine and basic biology. We used a high ‐throughput siRNA screening assay in the initiation phase of reprogramming for 784 genes belonging to kinase and phosphatase families and identified 68 repressors and 22 effectors. Six new candidates belonging to the family of the G protein–coupled receptors (GPCRs) were identified suggesting an important role for this key signalling pathway during somatic cell induced reprogra...
Source: Stem Cells - December 4, 2018 Category: Stem Cells Authors: Irina Neganova, Lewis Cotts, Peter Banks, Katja Gassner, Lyle Armstrong, Graham Ladds, Majlinda Lako Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research

Cai's Neiyi Prescription promotes apoptosis and inhibits inflammation in endometrial stromal cells with endometriosis through inhibiting USP10
AbstractTo observe the effect of Cai's Neiyi Prescription (CNYP) on the apoptosis and inflammation in endometrial stromal cells with endometriosis (EM) bothin vivo andin vitro, EM model rats and endometrial stromal cells were treated with CNYP and the level of USP10, p ‐ERK1/2, ERK1/2, and apoptosis‐related protein as well as the levels of proinflammatory factors were measured by Western blotting and ELISA, respectively. Rats with surgically induced EM showed increased USP10 expression and ERK/2 activation. Intragastric administration of CNYP granule significa ntly inhibited EM‐induced ERK1/2 activation and expressio...
Source: Biotechnology and Applied Biochemistry - December 4, 2018 Category: Biochemistry Authors: Shuangdi Li, Yaping Zhu, Tingting Zhang, Yuanyuan Hang, Qiong Chen, Yuli Jin Tags: Original Article Source Type: research

EDG5 plays an important role in induction and maintenance of pluripotency
Graphical abstract summarizing the main findings generated from this study. AbstractDirect reprogramming of human somatic cells toward induced pluripotent stem cells holds great promise for regenerative medicine and basic biology. We used a high ‐throughput siRNA screening assay in the initiation phase of reprogramming for 784 genes belonging to kinase and phosphatase families and identified 68 repressors and 22 effectors. Six new candidates belonging to the family of the G protein–coupled receptors (GPCRs) were identified suggesting an important role for this key signalling pathway during somatic cell induced reprogra...
Source: Stem Cells - December 4, 2018 Category: Stem Cells Authors: Irina Neganova, Lewis Cotts, Peter Banks, Katja Gassner, Lyle Armstrong, Graham Ladds, Majlinda Lako Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research

Involvement of the STAT5-CyclinD/CDK4-pRb pathway in beta cell proliferation stimulated by prolactin during pregnancy.
Abstract During pregnancy, maternal pancreatic beta cells undergo a compensatory expansion in response to the state of insulin resistance, where prolactin (PRL) plays a major role. Retinoblastoma protein (Rb) has been shown to critically regulate islet proliferation and function. The aim of the study was to explore the role of Rb in beta cell mass expansion during pregnancy. During pregnancy, expression of Rb, phospho-Rb (p-Rb), p107 and E2F1 increased, while p130 decreased in maternal islets. With PRL stimulation, induction of Rb expression occurred mainly in the nucleus while p-Rb was predominantly in the cytopl...
Source: Am J Physiol Endocri... - December 4, 2018 Category: Endocrinology Authors: Zhao X, Xu Y, Wu Y, Zhang H, Shi H, Zhu H, Woo M, Wu X Tags: Am J Physiol Endocrinol Metab Source Type: research

B7H6 is a functional ligand for NKp30 in rat and cattle and determines NKp30 reactivity towards human cancer cell lines.
This article is protected by copyright. All rights reserved. PMID: 30512185 [PubMed - as supplied by publisher]
Source: European Journal of Immunology - December 4, 2018 Category: Allergy & Immunology Authors: Bjørnsen EG, Thiruchelvam-Kyle L, Hoelsbrekken SE, Henden C, Saether PC, Boysen P, Daws MR, Dissen E Tags: Eur J Immunol Source Type: research

Self-targeted knockdown of CD44 improves cisplatin sensitivity of chemoresistant non-small cell lung cancer cells
ConclusionsThese results suggest that self-targeted downregulation of chemoresistance-associated cell surface proteins during chemotherapy is an effective therapeutic strategy for overcoming the chemoresistance of NSCLC cells.
Source: Cancer Chemotherapy and Pharmacology - December 4, 2018 Category: Cancer & Oncology Source Type: research

GSE115385 Transcriptomal analysis of the impact of ASAH1 inhibition on skin metastatic melanoma cells
Contributors : J Leclerc ; N Nottet ; C Bertolotto ; R BallottiSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo assess the impact of ASAH1 inhibition skin metastatic melanoma, we transfected three different human skin metastatic melanoma cell lines (501Mel, WM3912 and WM8) with a siRNA designed against ASAH1 (50nM) or a siRNA control (50nM) for 48h.
Source: GEO: Gene Expression Omnibus - December 3, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Initial evidence of functional siRNA machinery in dinoflagellates
This study was aimed to adopt the RNA interference (RNAi) gene-silencing tool for dinoflagellate research, and to investigate the potential effects of RNAi-based silencing of proton-pump rhodopsin and CO2-fixing enzyme Rubisco encoding genes in dinoflagellates. It was found that RNAi treatment caused a significant decrease in growth rate in both species. Compared with the non- endogenous target (GFP-siRNA) and the blank control, RNAi treatments also suppressed the expression of the target genes. These results constitute the first experimental evidence of the existence and operation of siRNA in two species of dinoflagellate...
Source: Harmful Algae - December 2, 2018 Category: Environmental Health Source Type: research

Effective Lipidoid Nanoparticle Delivery In Vivo of siRNA Targeting Kappa Light Chain Production in a Murine Xenograft Model
CONCLUSIONS: We have previously shown that siIGKC, a pool of siRNA directed at consensus sequences in the LC constant region gene, can significantly reduce LC production in clonal plasma cells from patients, in human myeloma cell lines, and in vivo in a flank plasmacytoma xenograft model. In this work, we show that 8B-3 is a promising LPN for delivery of siRNA to human plasma cells and, when loaded with siIGKC, can with relative safety significantly reduce circulating LC in the NSG JJN3 IP model after 3 daily IP injections despite rapid tumor growth. We also show the utility of the NSG JJN3 IP model for the study of LC dir...
Source: Blood - November 21, 2018 Category: Hematology Authors: Ma, X., Zhou, P., Kugelmass, A., Toskic, D., Warner, M., Lee, L. X., Fogaren, T., Wang, M., Li, Y., Yang, L., Xu, Q., Comenzo, R. Tags: 652. Myeloma: Pathophysiology and Pre-Clinical Studies, excluding Therapy: Poster II Source Type: research

SLN124, a Galnac-siRNA Conjugate Targeting TMPRSS6, for the Treatment of Iron Overload and Ineffective Erythropoiesis Such As in Beta-Thalassemia
Accumulation of excess iron in tissues causes organ damage and dysfunction and may lead to serious clinical consequences including liver cirrhosis, diabetes, growth retardation and heart failure. Iron overload is a major health threat in iron loading anemias, like beta-thalassemia, myelodysplastic syndrome and in hereditary hemochromatosis. In patients with beta-thalassemia major, iron overload develops due to frequent blood transfusions to control the severe anemia. In addition, iron overload also occurs in patients with beta thalassemia intermedia (non-transfusion dependent beta-thalassemia). In the later cases, iron ove...
Source: Blood - November 21, 2018 Category: Hematology Authors: Altamura, S., Altamura, S., Muckenthaler, M. U., Dames, S., Frauendorf, C., Schubert, S., Aleku, M., Vadolas, J., Grigoriadis, G., Zugel, U. Tags: 102. Regulation of Iron Metabolism: Poster II Source Type: research

Inhibition of YAP with siRNA prevents cartilage degradation and ameliorates osteoarthritis development
AbstractThe Hippo/YAP signaling pathway is important for mediating organ size and tissue homeostasis, but its role in osteoarthritis (OA) remains unclear. We aimed to investigate the role of Hippo/YAP signaling pathway in OA development. YAP expression in OA cartilage was assessed by immunohistochemistry, RT-qPCR, and Western blotting. The effects of YAP overexpression or knockdown on gene expression related to chondrocyte hypertrophy induced by IL-1 β were examined. The in vivo effects of YAP inhibition were studied. Subchondral bone was analyzed by micro-CT. YAP was increased in mice and human OA articular cartilage and...
Source: Journal of Molecular Medicine - November 21, 2018 Category: Molecular Biology Source Type: research

GSE118228 Gene expression data from human endometrial cancer cells treated with siRNA targeting Efp
Contributors : Wataru Sato ; Kazuhiro Ikeda ; Kuniko Horie-Inoue ; Satoshi InoueSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo examine the role of Efp in endometrial cancer cells, Ishikawa and HEC-1A cells were treated with siRNA targeting Efp (siEfp) or control siRNA (siControl). Microarray analysis showed that Efp knockdown represses NF- κB-signaling pathway-associated genes.
Source: GEO: Gene Expression Omnibus - November 20, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

A DR4 capturer with AKT siRNA for the synergetic enhancement of death receptor-mediated apoptosis
Chem. Commun., 2018, Advance Article DOI: 10.1039/C8CC06796A, CommunicationWei Pan, Shaohua Gong, Yanhua Li, Huiwen Zhang, Na Li, Bo Tang A DR4 capturer with AKT siRNA was developed for the synergetic enhancement of death receptor-mediated apoptosis. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - November 15, 2018 Category: Chemistry Authors: Wei Pan Source Type: research

5'-triphosphate siRNA targeting HBx elicits a potent anti-HBV immune response in pAAV-HBV transfected mice.
Abstract RNA with 5'-triphosphate (3p-RNA) is recognized by RNA sensor RIG-I (retinoic acid-inducible gene I protein). Previously, we reported that small interfering RNA targeting HBx (3p-siHBx) could confer potent anti-hepatitis B virus (HBV) efficacy via HBx silencing and RIG-I activation. However, the characteristics of innate and adaptive immunity especially exhaustion profiles in the liver microenvironment in response to 3p-siHBx therapy have not been fully elucidated. Here, we observed that 3p-siHBx more significantly inhibited HBV replication in vivo. 3p-siHBx enhanced natural killer (NK) cell activation wi...
Source: Antiviral Research - November 15, 2018 Category: Virology Authors: Han Q, Hou Z, Yin C, Zhang C, Zhang J Tags: Antiviral Res Source Type: research

A DR4 capturer with AKT siRNA for the synergetic enhancement of death receptor-mediated apoptosis.
Abstract A graphene-based nanocarrier modified with death receptor 4 (DR4) antibody and AKT siRNA has been developed, which can synergistically strengthen death receptor-mediated apoptosis and enhance the cancer therapeutic effect in vivo. PMID: 30430148 [PubMed - as supplied by publisher]
Source: Chemical Communications - November 15, 2018 Category: Chemistry Authors: Pan W, Gong S, Li Y, Zhang H, Li N, Tang B Tags: Chem Commun (Camb) Source Type: research

Local Silencing of Connective Tissue Growth Factor by siRNA/Peptide Improves Dermal Collagen Arrangements
Conclusion:CTGF expression was down-regulated to 40% of control by CTGF siRNA/KALA (1:24) complexes (p <  0.01) and collagen lattice contraction was inhibited. However, down-regulated of CTGF by CTGF siRNA/MPG∆NLS complexes was not statistically significant. CTGF KALA-treated wound appeared with well formed-basket weave pattern of collagen fibrils with mean diameter of 128  ± 22 nm (n = 821). Mismatch siRNA/KALA-treated wound showed a high frequency of parallel small diameter fibrils (mean 90 ± 20 nm, n = 563).Conclusion:Controlling over-expression of CTGF by peptide-mediated siRNA delivery cou...
Source: Tissue Engineering and Regenerative Medicine - November 14, 2018 Category: Biotechnology Source Type: research

Controlling HCV infection by targeting its translation initiation site in PBMCs using siRNA; In Vitro.
Abstract Hepatitis C virus infection and its complications are among the leading public health challenges, the emergence of drug-resistant variants is expected to be a major problem. A combinatorial small interfering RNA (siRNA) could be a novel triple therapy that could be suitable for genotype 4. HCV believed to be hepatotropic, but there is liable evidence about its replication in peripheral blood mononuclear cells (PBMC) of chronic HCV infected patients. These cells act as an extra-hepatic reservoir for viral recurrence and persistence, patients with HCV-RNA in PBMC showed a significantly lower response to the...
Source: Infectious Disorders Drug Targets - November 14, 2018 Category: Infectious Diseases Authors: Youssef SS, Elemeery MN, Eldein SS, Ghareeb DA Tags: Infect Disord Drug Targets Source Type: research