Query: siRNA

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Total 16423 results found since Jan 2013.

Suppression of trophoblast cell surface antigen 2 enhances proliferation and migration in liver fluke-associated cholangiocarcinoma.
CONCLUSION: We provide new evidence that TROP2 is epigenetically down-regulated and operates as a negative regulator of cell proliferation and migration in liver fluke-associated CCA. PMID: 31320172 [PubMed]
Source: Annals of Hepatology - July 22, 2019 Category: Gastroenterology Tags: Ann Hepatol Source Type: research

Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro.
CONCLUSIONS Our results confirm that OMT alleviates transdifferentiation of cardiac fibroblasts to myofibroblasts induced by aldosterone via activating the Nrf2/Keap1 pathway in vitro. PMID: 31325292 [PubMed - in process]
Source: Medical Science Monitor - July 21, 2019 Category: Research Tags: Med Sci Monit Source Type: research

Role of B-Cell Lymphoma 2 Ovarian Killer (BOK) in Acute Toxicity of Human Lung Epithelial Cells Caused by Cadmium Chloride.
CONCLUSIONS These findings support a role for BOK in CdCl₂-induced DNA damage and cell death. PMID: 31323016 [PubMed - in process]
Source: Medical Science Monitor - July 21, 2019 Category: Research Tags: Med Sci Monit Source Type: research

Susceptibility of Enterovirus-D68 to RNAi-mediated antiviral knockdown
Publication date: Available online 20 July 2019Source: Antiviral ResearchAuthor(s): Nicholas Klaiber, Michael A. McVoy, Wei ZhaoAbstractEnterovirus D68 (EV-D68) represents an emerging pathogen which has demonstrated a capacity for causing epidemic illness in pediatric and immunocompromised patients. With no effective antiviral treatment available, therapeutic interventions are currently limited to supportive care. Utilizing available genomic sequences from the 2014 B3 Epidemic EV-D68 clade and the 1962 Fermon EV-D68 strains, we performed in silico comparative genomic analysis, identifying several islands of phylogenetic co...
Source: Antiviral Therapy - July 21, 2019 Category: Virology Source Type: research

Lysosomal cysteine protease cathepsin S is involved in cancer cell motility by regulating store-operated Ca2+ entry
In conclusion, we have demonstrated for the first time that the lysosomal cysteine protease, CTSS, plays an important role in mediating Ca2+ homeostasis by regulating STIM1 trafficking, which leads to the suppression of cell migration and invasion.
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - July 21, 2019 Category: Molecular Biology Source Type: research

Inhibition of ubiquitin ‑specific protease 14 promotes connexin 32 internalization and counteracts cisplatin cytotoxicity in human ovarian cancer cells.
Inhibition of ubiquitin‑specific protease 14 promotes connexin 32 internalization and counteracts cisplatin cytotoxicity in human ovarian cancer cells. Oncol Rep. 2019 Jul 15;: Authors: Luo H, Wang X, Ge H, Zheng N, Peng F, Fu Y, Tao L, Wang Q Abstract Although cisplatin is one of the most accepted therapies for ovarian cancer, recurrence and drug resistance remain problematic. Both the ubiquitin‑proteasome system (UPS) and connexin (Cx) are closely related to tumor progression. However, the role of ubiquitin‑specific protease 14 (USP14) and Cx in mediating drug resistance remains unclear. In...
Source: Oncology Reports - July 21, 2019 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Tetramethylpyrazine downregulates transcription of the CXC receptor  4 (CXCR4) via nuclear respiratory factor‑1 (Nrf‑1) in WERI‑Rb1 retinoblastoma cells.
Tetramethylpyrazine downregulates transcription of the CXC receptor 4 (CXCR4) via nuclear respiratory factor‑1 (Nrf‑1) in WERI‑Rb1 retinoblastoma cells. Oncol Rep. 2019 Jul 15;: Authors: Wu N, Yang Y, Yu N, Wu Y, Han X, Chen S, Zhang J, Chen X, Wu C, Yang M, Qiu J, Ge J, Yu K, Zhuang J Abstract Tetramethylpyrazine (TMP; an extract of the Chinese herbal medicine, Chuanxiong) has been shown to exert remarkable antiretinoblastoma (RB) effects. Based on our previous study, the target gene was found to be C‑X‑C chemokine receptor type 4 (CXCR4). CXCR4 is a prognostic marker in various types of c...
Source: Oncology Reports - July 21, 2019 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MicroRNA ‑let‑7a inhibition inhibits LPS‑induced inflammatory injury of chondrocytes by targeting IL6R.
MicroRNA‑let‑7a inhibition inhibits LPS‑induced inflammatory injury of chondrocytes by targeting IL6R. Mol Med Rep. 2019 Jul 12;: Authors: Sui C, Zhang L, Hu Y Abstract Osteoarthritis (OA) is a type of degenerative joint disease that affects the health of the elderly. OA is characterized by articular cartilage degradation and joint inflammation. The present study aimed to investigate the role and mechanism of microRNA‑let‑7a (Let‑7a) in OA by examining its role in lipopolysaccharide (LPS)‑induced cartilage inflammatory injury in ATDC5 cells. ATDC5 cells were treated with various concentr...
Source: Molecular Medicine Reports - July 21, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

MPZL1 is highly expressed in advanced gallbladder carcinoma and promotes the aggressive behavior of human gallbladder carcinoma GBC ‑SD cells.
In conclusion, the present study showed that the expression and protein levels of MPZL1 were significantly higher in gallbladder carcinoma tissues, especially in patients diagnosed with advanced tumor stages. Overexpression of MPZL1 may have promoted the invasion, metastasis, proliferation and survival of GBC‑SD cells. PMID: 31322261 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - July 21, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

ATRA protects skin fibroblasts against UV ‑induced oxidative damage through inhibition of E3 ligase Hrd1.
In conclusion, the present data demonstrated that ATRA protected skin fibroblasts against UV‑induced oxidative damage through inhibition of E3 ligase Hrd1. PMID: 31322186 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - July 21, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

MicroRNA-191 targets CCAAT/enhanced binding protein β and functions as an oncogenic molecule in human non-small cell lung carcinoma cells.
Authors: Li F, Wen J, Shi J, Wang Y, Yang F, Liu C Abstract The aberrant expression of microRNAs (miRs) may be involved in tumor growth and progression in human non-small cell lung carcinoma (NSCLC). The present study aimed to investigate the potential roles of miR-191 in NSCLC. Western blotting and reverse transcription-quantitative polymerase chain reaction were performed to assess protein and/or mRNA levels. Scratch wound healing and transwell assays were performed to determine the NSCLC cell migration and invasion. A luciferase demonstrated that CCAAT/enhanced binding protein β (C/EBPβ) was a target of miR-19...
Source: Experimental and Therapeutic Medicine - July 20, 2019 Category: General Medicine Tags: Exp Ther Med Source Type: research

GSE117415 RNA-seq in SUNE-1 cells after downregulation of DANCR expression
Contributors : Xin Wen ; Jun Ma ; Na LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo explore whether DANCR plays a role in nasopharyngeal carcinoma tumorigenesis, a RNA-seq analysis was performed to compare the gene expression profiles of DANCR siRNA and control groups.
Source: GEO: Gene Expression Omnibus - July 20, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Intranasal Delivery of lincRNA-Cox2 siRNA Loaded Extracellular Vesicles Decreases Lipopolysaccharide-Induced Microglial Proliferation in Mice
In this study, we found thatlincRNA-Cox2 controls the expression of a set of cell cycle genes in lipopolysaccharide (LPS)-stimulated microglial cells. Our in vitro study suggested that knocking downlincRNA-Cox2 reversed LPS-induced microglial proliferation. In addition, our in vivo study demonstrated that intranasally delivered lincRNA-Cox2-siRNA loaded EVs could reach the brain resulting in a significant decrease in the expression oflincRNA-Cox2 in the microglia. Importantly, lincRNA-Cox2-siRNA loaded EVs also decreased LPS-induced microglial proliferation in mice. These findings indicate that intranasal delivery of EV-lo...
Source: Journal of NeuroImmune Pharmacology - July 20, 2019 Category: Drugs & Pharmacology Source Type: research

Triptolide enhances the sensitivity of pancreatic cancer PANC-1 cells to gemcitabine by inhibiting TLR4/NF- κB signaling.
CONCLUSIONS: TPL enhances the sensitivity of pancreatic cancer PANC-1 cells to GEM by inhibiting TLR4/NF-κB signaling. PMID: 31312385 [PubMed]
Source: American Journal of Translational Research - July 19, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Inhibition of miR-9 attenuates fibroblast proliferation in human hyperplastic scar by regulating TGF- β1.
In conclusion, this study showed that miR-9 plays an important role in the proliferation of fibroblasts by regulating TGF-β1 expression in HS tissue. PMID: 31312375 [PubMed]
Source: American Journal of Translational Research - July 19, 2019 Category: Research Tags: Am J Transl Res Source Type: research

NADPH oxidase 4 is correlated with gastric cancer progression and predicts a poor prognosis.
In conclusion, NOX4 is related to gastric cancer development and predicts a poor prognosis. NOX4 may play an essential role in the progression of gastric cancer, and is a promising target for the prevention and treatment of gastric cancer. PMID: 31312363 [PubMed]
Source: American Journal of Translational Research - July 19, 2019 Category: Research Tags: Am J Transl Res Source Type: research

Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development.
Abstract Cellular models for siRNA and small molecule high-throughput screening have been widely used in the last decade to identify targets for drug discovery. As an example, we present a twofold readout approach based on cell viability and multipolar phenotype. To maximize the discovery of potential targets and at the same time reduce the number of false positives in our dataset, we have combined focused and rationally designed custom siRNA libraries with small molecule inhibitor libraries. Here we describe a cellular model for centrosome amplification as an example of how to design and perform a multiple readou...
Source: Mol Biol Cell - July 19, 2019 Category: Molecular Biology Authors: Drosopoulos K, Linardopoulos S Tags: Methods Mol Biol Source Type: research

Ca2+/Calmodulin-dependent kinase II delta B is essential for cardiomyocyte hypertrophy and complement gene expression after LPS and HSP60 stimulation in vitro.
This study evaluated mechanisms underlying inflammatory response and cardiomyocyte hypertrophy associated with bacterial lipopolysaccharide (LPS)- or heat shock protein 60 (HSP60)-induced Toll-like receptor (TLR) stimulation and the effect of a small interfering RNA (siRNA) against Ca2+/calmodulin-dependent kinase II delta B (CaMKIIδB) on these outcomes. Our results showed that treatment with HSP60 or LPS (TLR agonists) induced cardiomyocyte hypertrophy and complement system C3 and factor B gene expression. In vitro silencing of CaMKIIδB prevented complement gene transcription and cardiomyocyte hypertrophy associated wit...
Source: Braz J Med Biol Res - July 19, 2019 Category: Research Authors: Cruz Junho CV, Trentin-Sonoda M, Alvim JM, Gaisler-Silva F, Carneiro-Ramos MS Tags: Braz J Med Biol Res Source Type: research

{beta}-Arrestin-1 inhibits glucocorticoid receptor turnover and alters glucocorticoid signaling Signal Transduction
In this study, we discovered a novel protein interaction between the glucocorticoid receptor and β-arrestin-1, a scaffold protein with a well-established role in G protein–coupled receptor signaling. Using co-immunoprecipitation and in situ proximity ligation assays in A549 cells, we observed that β-arrestin-1 and unliganded GR interact in the cytoplasm and that, following glucocorticoid binding, the protein complex is found in the nucleus. We show that siRNA-mediated β-arrestin-1 knockdown alters GR protein turnover by up-regulating the E3 ubiquitin ligase Pellino-1, which catalyzes GR ubiquitination and thereby mark...
Source: Journal of Biological Chemistry - July 19, 2019 Category: Chemistry Authors: Maria G. Petrillo, Robert H. Oakley, John A. Cidlowski Tags: Signal Transduction Source Type: research

PSMA5 promotes the tumorigenic process of prostate cancer and is related to bortezomib resistance
In this study, we investigated whether PSMA5 is associated with the tumorigenic progression and the interaction of PSMA5 with bortezomib in PCa. We knocked down PSMA5 with siRNA and studied the changes in cell viability and motility with Cell Counting Kit-8, quantitative PCR, fluorescence-activated cell sorting, scratch, and invasion assays. We also investigated the effect of PSMA5 in PCa cells treated with bortezomib and in those that are resistant to bortezomib. We found that silencing PSMA5 inhibited cell proliferation, induced apoptosis, restricted cell migration and invasion, and demonstrated a coordinated effect with...
Source: Anti-Cancer Drugs - July 18, 2019 Category: Cancer & Oncology Tags: Preclinical Reports Source Type: research

Apigenin Protects Against Renal Tubular Epithelial Cell Injury and Oxidative Stress by High Glucose via Regulation of NF-E2-Related Factor 2 (Nrf2) Pathway.
CONCLUSIONS Apigenin protects renal tubular epithelial cells against high glucose-induced injury through suppression of oxidative stress and inflammation via activation of the Nrf2 pathway. PMID: 31309931 [PubMed - in process]
Source: Medical Science Monitor - July 18, 2019 Category: Research Tags: Med Sci Monit Source Type: research

Flavonoids from Livistona chinensis fruit ameliorates LPS/D-GalN-induced acute liver injury by inhibiting oxidative stress and inflammation
This study investigated the hepatoprotective effect and mechanism of flavonoids from Livistona chinensis fruit (FLCF). Mice received FLCF pre-treatment for 7 days and induced liver injury with LPS+D-GalN. RAW264.7 cells were triggered inflammation by LPS and received FLCF for 24 h. The result showed that FLCF ameliorated the liver pathology in mice, decreased the levels of AST, ALT, MDA, but increased SOD and GSH. Moreover, FLCF inhibited TLR4/NF-κB signaling, while activated Nrf2/HO-1 signaling in LPS+D-GalN mice. These effects were consistent with the in vitro data revealing that FLCF suppressed TLR4/NF-κB signalin...
Source: Journal of Functional Foods - July 18, 2019 Category: Nutrition Source Type: research

Identification of TMEM206 proteins as pore of PAORAC/ASOR acid-sensitive chloride channels
Acid-sensing ion channels have important functions in physiology and pathology, but the molecular composition of acid-activated chloride channels had remained unclear. We now used a genome-wide siRNA screen to molecularly identify the widely expressed acid-sensitive outwardly-rectifying anion channel PAORAC/ASOR. ASOR is formed by TMEM206 proteins which display two transmembrane domains (TMs) and are expressed at the plasma membrane. Ion permeation-changing mutations along the length of TM2 and at the end of TM1 suggest that these segments line ASOR ’s pore. While not belonging to a gene family, TMEM206 has orthologs in ...
Source: eLife - July 18, 2019 Category: Biomedical Science Tags: Cell Biology Structural Biology and Molecular Biophysics Source Type: research

Endogenous retrovirus-encoded Syncytin-2 contributes to exosome-mediated immunosuppression of T cells.
Abstract Modulation of the activation status of immune cell populations during pregnancy depends on placental villous cytotrophoblast (VCT) cells and the syncytiotrophoblast (STB) layer. Failure in the establishment of this immunoregulatory function leads to pregnancy complications. Our laboratory has been studying Syncytin-2 (Syn-2), an endogenous retroviral protein expressed in placenta and on the surface of placental exosomes. This protein plays an important role in STB formation through its fusogenic properties, but also possesses an active immunosuppressive domain (ISD). Considering that Syn-2 expression is i...
Source: Biology of Reproduction - July 18, 2019 Category: Reproduction Medicine Authors: Lokossou AG, Toudic C, Nguyen PT, Elisseeff X, Vargas A, Rassart É, Lafond J, Leduc L, Bourgault S, Gilbert C, Scorza T, Tolosa J, Barbeau B Tags: Biol Reprod Source Type: research

Glycoproteomic Analysis of the Aortic Extracellular Matrix in Marfan Patients.
CONCLUSIONS: Our glycoproteomics analysis revealed that MFAP4 glycosylation is enhanced, as well as its expression during the advanced, aneurysmal stages of MFS compared with control aneurysms from patients without MFS. PMID: 31315432 [PubMed - as supplied by publisher]
Source: Arteriosclerosis, Thrombosis and Vascular Biology - July 18, 2019 Category: Cardiology Authors: Yin X, Wanga S, Fellows A, Barallobre-Barreiro J, Lu R, Davaapil H, Franken R, Fava M, Baig F, Skroblin P, Xing Q, Koolbergen DR, Groenink M, Zwinderman AH, Balm R, de Vries CJM, Mulder BJM, Viner R, Jahangiri M, Reinhardt DP, Sinha S, de Waard V, Mayr M Tags: Arterioscler Thromb Vasc Biol Source Type: research

Autophagy induction by the pathogen receptor NECTIN4 and sustained autophagy contribute to peste des petits ruminants virus infectivity.
Abstract Macroautophagy/autophagy is an essential cellular response in the fight against intracellular pathogens. Although some viruses can escape from or utilize autophagy to ensure their own replication, the responses of autophagy pathways to viral invasion remain poorly documented. Here, we show that peste des petits ruminants virus (PPRV) infection induces successive autophagic signalling in host cells via distinct and uncoupled molecular pathways. Immediately upon invasion, PPRV induced a first transient wave of autophagy via a mechanism involving the cellular pathogen receptor NECTIN4 and an AKT-MTOR-depende...
Source: Autophagy - July 18, 2019 Category: Cytology Authors: Yang B, Xue Q, Guo J, Wang X, Zhang Y, Guo K, Li W, Chen S, Xue T, Qi X, Wang J Tags: Autophagy Source Type: research

Long noncoding RNA IGF2AS regulates high ‐glucose induced apoptosis in human retinal pigment epithelial cells
AbstractHigh ‐glucose‐induced retinal tissue impairment is the major pathological phenotype of diabetic retinopathy. In an in vitro diabetic apoptosis cell model, we evaluated the function of long noncoding RNA, insulin growth factor 2 antisense (IGF2‐AS) in high‐glucose‐injured human retinal pigment e pithelial cells. A human retinal pigment epithelial cell line, ARPE‐19 was incubated with high‐glucose in vitro to induce apoptosis. SiRNA‐mediated IGF2‐AS downregulation was conducted in ARPE‐19 cells to evaluate its effect on high‐glucose induced apoptosis, assessed by a TUNEL assay. q RT‐PCR and we...
Source: IUBMB Life - July 18, 2019 Category: Research Authors: Xiaoyi Yu, Yingzi Luo, Gangyi Chen, Hong Liu, Ni Tian, Xiaoting Zen, Qiuhong Liu Tags: Research Communication Source Type: research

MicroRNA ‐155 inhibition up‐regulates LEPR to inhibit osteoclast activation and bone resorption via activation of AMPK in alendronate‐treated osteoporotic mice
AbstractOsteoporosis is characterized by a progressive increase in bone fragility, leading to low bone mass and structural deterioration of bone tissue. MicroRNA ‐155 (miR‐155) is highly expressed in osteoporosis. Thus, the current study aimed to investigate the effect of miR‐155 on the inhibition of osteoclast activation and bone resorption by targeting leptin receptor (LEPR) through the adenosine monophosphate activated protein kinase (AMPK) pathway in alendronate‐treated osteoporotic mice. An osteoporosis mouse model was established to examine the bone tension and bone density and the expression of miR‐155 in ...
Source: IUBMB Life - July 18, 2019 Category: Research Authors: Zhongxuan Mao, Yihai Zhu, Weimin Hao, Chao Chu, Hongmin Su Tags: RESEARCH COMMUNICATION Source Type: research

Nanotechnology-based siRNA Delivery Strategies for Metastatic Colorectal Cancer Therapy
Publication date: Available online 16 July 2019Source: International Journal of PharmaceuticsAuthor(s): Ana Rita Sousa, Ana Vanessa Oliveira, Maria José Oliveira, Bruno SarmentoAbstractColorectal cancer (CRC) is the second most common cause of cancer-related death in the world, mainly due to distant metastases events. The metastatic CRC (mCRC) stages are resistant to the therapeutic recommended. Therefore, it urges the development of more efficient strategies to impair the disease. Small interfering RNA (siRNA) is a well-known silencing tool with impact on targeted cancer therapy, even though in vivo challenges difficult ...
Source: International Journal of Pharmaceutics - July 17, 2019 Category: Drugs & Pharmacology Source Type: research

Nanowire-array-based gene electro-transfection system driven by human-motion operated triboelectric nanogenerator
Publication date: Available online 15 July 2019Source: Nano EnergyAuthor(s): Chengbin Yang, Guang Yang, Qingling Ouyang, Shuangyang Kuang, Peiyi Song, Gaixia Xu, Daniel Puiu Poenar, Guang Zhu, Ken-Tye Yong, Zhong Lin WangAbstractElectro-transfection serves as a promising non-viral gene delivery approach, but its performance faces several drawbacks associated with the high-voltage electrical pulses involved, which may adversely affect the cell viability. Here we developed a novel TENG (triboelectric nanogenerator)-driven nanowire electrode array (T-NEA) platform to facilitate the electroporation-based siRNA delivery into bo...
Source: Nano Energy - July 17, 2019 Category: Nanotechnology Source Type: research

GSE129736 siRNA in cer7, rst1 and ripr mutants
Contributors : Heike Lange ; Dominique GagliardiSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Arabidopsis thalianaidentify and compare siRNAs in wildtype and mutants of the RNA exosome
Source: GEO: Gene Expression Omnibus - July 17, 2019 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

MicroRNA-145 suppresses osteogenic differentiation of human jaw bone marrow mesenchymal stem cells partially via targeting semaphorin 3A.
Conclusions: Our data show that miR-145 directly targets SEMA3A, and also suggest miR-145 as a suppressor, plays an important role in the osteogenic differentiation of h-JBMMSCs. PMID: 31305177 [PubMed - as supplied by publisher]
Source: Connective Tissue Research - July 17, 2019 Category: Research Tags: Connect Tissue Res Source Type: research

IL-19 Up-Regulates Mucin 5AC Production in Patients With Chronic Rhinosinusitis via STAT3 Pathway
This study was conducted to determine the roles and mechanisms of IL-19, a member of the IL-20 subfamily, in regulating MUC5AC production in chronic rhinosinusitis (CRS). We analyzed the expression of mucin and MUC5AC in the nasal mucosa of patients with CRS by periodic acid Schiff (PAS) staining and immunohistochemical examination. Real-time quantitative PCR, ELISA, confocal microscopy and western blotting were used to measure MUC5AC expression in primary human nasal epithelium cells (PHNECs) stimulated with recombinant human IL-19 (rhIL-19), IL-19 receptor siRNA transfection or control. The involvement of the STAT3 signa...
Source: Frontiers in Immunology - July 17, 2019 Category: Allergy & Immunology Source Type: research

Nrf2/HO-1 signaling pathway participated in the protection of hydrogen sulfide on neuropathic pain in rats.
Abstract Neuropathic pain is evoked by aberrant sensory processing in the peripheral or central nervous system, which is characterized by persistent pain, tactile allodynia, or hyperalgesia. Neuroinflammation is associated with the initiation and maintenance of persistent pain in both the peripheral and central nervous systems. Hydrogen sulfide plays important regulatory roles in different physiological and pathological conditions. Therefore, we investigated the effect of hydrogen sulfide on allodynia, hyperalgesia and cytokine release in rats with neuropathic pain and the related regulatory mechanism. Neuropathic...
Source: International Immunopharmacology - July 17, 2019 Category: Allergy & Immunology Authors: Chen H, Xie K, Chen Y, Wang Y, Wang Y, Lian N, Zhang K, Yu Y Tags: Int Immunopharmacol Source Type: research

Viruses, Vol. 11, Pages 651: Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication
We report that MOV10 down-regulation, using siRNA, shRNA, and CRISPR/Cas9 gene editing technology, resulted in increased levels of HBV DNA, HBV pre-genomic RNA, and HBV core protein. In contrast, MOV10 over-expression reduced HBV DNA, HBV pre-genomic RNA, and HBV core protein. These effects were consistent in all tested cell lines, providing strong evidence for the involvement of MOV10 in the HBV life cycle. We demonstrated that MOV10 does not interact with HBV-core. However, MOV10 binds HBV pgRNA and this interaction does not affect HBV pgRNA decay rate. We conclude that the restriction of HBV by MOV10 is mediated through...
Source: Viruses - July 17, 2019 Category: Virology Authors: Maritza N. Puray-Chavez Mahmoud H. Farghali Vincent Yapo Andrew D. Huber Dandan Liu Tanyaradzwa P. Ndongwe Mary C. Casey Thomas G. Laughlin Mark Hannink Philip R. Tedbury Stefan G. Sarafianos Tags: Article Source Type: research

Long non-coding RNA H19 is responsible for the progression of lung adenocarcinoma by mediating methylation-dependent repression of CDH1 promoter.
Abstract Lung adenocarcinoma is a common histologic type of lung cancer with a high death rate globally. Increasing evidence shows that long non-coding RNA H19 (lncRNA H19) and CDH1 methylation are involved in multiple tumours. Here, we tried to investigate whether lncRNA H19 or CDH1 methylation could affect the development of lung adenocarcinoma. First, lung adenocarcinoma tissues were collected to detect CDH1 methylation. Then, the regulatory mechanisms of lncRNA H19 were detected mainly in concert with the treatment of overexpression of lncRNA H19, siRNA against lncRNA H19, overexpression of CDH1 and demethylat...
Source: J Cell Mol Med - July 17, 2019 Category: Molecular Biology Authors: Gao LM, Xu SF, Zheng Y, Wang P, Zhang L, Shi SS, Wu T, Li Y, Zhao J, Tian Q, Yin XB, Zheng L Tags: J Cell Mol Med Source Type: research

Research Articles Calpain 9 as a therapeutic target in TGF{beta}-induced mesenchymal transition and fibrosis
Fibrosis is a common pathologic outcome of chronic disease resulting in the replacement of normal tissue parenchyma with a collagen-rich extracellular matrix produced by myofibroblasts. Although the progenitor cell types and cellular programs giving rise to myofibroblasts through mesenchymal transition can vary between tissues and diseases, their contribution to fibrosis initiation, maintenance, and progression is thought to be pervasive. Here, we showed that the ability of transforming growth factor–β (TGFβ) to efficiently induce myofibroblast differentiation of cultured epithelial cells, endothelial cells...
Source: Science Translational Medicine - July 17, 2019 Category: Biomedical Science Authors: Kim, D. H., Beckett, J. D., Nagpal, V., Seman-Senderos, M. A., Gould, R. A., Creamer, T. J., MacFarlane, E. G., Chen, Y., Bedja, D., Butcher, J. T., Mitzner, W., Rouf, R., Hata, S., Warren, D. S., Dietz, H. C. Tags: Research Articles Source Type: research

ZYZ-803, a novel hydrogen sulfide-nitric oxide conjugated donor, promotes angiogenesis via cross-talk between STAT3 and CaMKII.
In this study, we investigated whether the signal transducer and activator of transcription 3 (STAT3) and Ca2+/CaM-dependent protein kinase II (CaMKII) signaling was involved in ZYZ-803-induced angiogenesis. Treatment with ZYZ-803 (1 μM) significantly increased the phosphorylation of STAT3 (Tyr705) and CaMKII (Thr286) in human umbilical vein endothelial cells (HUVECs), these two effects had a similar time course. Pretreatment with WP1066 (STAT3 inhibitor) or KN93 (CAMKII inhibitor) blocked ZYZ-803-induced STAT3/CAMKII activation and significantly suppressed the proliferation and migration of HUVECs. In addition, pretrea...
Source: Acta Pharmacologica Sinica - July 17, 2019 Category: Drugs & Pharmacology Authors: Xiong Y, Chang LL, Tran B, Dai T, Zhong R, Mao YC, Zhu YZ Tags: Acta Pharmacol Sin Source Type: research

EPHA2 feedback activation limits the response to PDE δ inhibition in KRAS-dependent cancer cells.
In this study, we explored how PDEδ inhibition was responded in KRAS mutant cancer cells, and identified KRAS mutant subset responsive to PDEδ inhibition. We first performed siRNA screen of KRAS growth dependency of a small panel of human cancer lines, and identified a subset of KRAS mutant cancer cells that were highly dependent on KRAS signaling. Among these cells, only a fraction of KRAS-dependent cells responded to PDEδ depletion, though KRAS plasma membrane association was effectively impaired. We revealed that the persistent RAF/MEK/ERK signaling seemed responsible for the lack of response to PDEδ depletion. A ki...
Source: Acta Pharmacologica Sinica - July 17, 2019 Category: Drugs & Pharmacology Authors: Chen YH, Lv H, Shen N, Wang XM, Tang S, Xiong B, Ding J, Geng MY, Huang M Tags: Acta Pharmacol Sin Source Type: research

PPAR γ participates in the acquisition of brain ischemic tolerance induced by ischemic preconditioning via GLT‐1 in vivo and in vitro
In conclusion, PPARγ participates in regulating GLT‐1 during the acquisition of BIT induced by IPC.This article is protected by copyright. All rights reserved.
Source: Journal of Neurochemistry - July 17, 2019 Category: Neuroscience Authors: Cong ‐Cong Zhao, Meng‐Yang Jiang, Ling‐Yan Zhang, Yu‐Yan Hu, Zhen‐Jie Hu, Meng‐Yue Zhang, Jie Qi, A‐Chou Su, Nan Lou, Xiao‐Hui Xian, Jing‐Ge Zhang, Wen‐Bin Li, Min Zhang Tags: Original Article Source Type: research

MiR-153 inhibits the resistance of lung cancer to gefitinib via modulating expression of ABCE1.
CONCLUSIONS: MiR-153 regulates gefitinib resistance by modulating expression of ABCE1 in lung cancers. Our findings may provide a worthwhile therapeutic target to reverse gefitinib resistance in lung cancers in the future. PMID: 31306106 [PubMed - as supplied by publisher]
Source: Cancer Biomarkers - July 16, 2019 Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research

Long non-coding RNA UCA1 regulates tumor growth by impairing let-7e-dependent HMGA2 repression in bladder cancer.
CONCLUSION: Our results demonstrate a lncRNA-UCA1/let-7e/HMGA2-dependent mechanism in bladder cancer and support lncRNA-UCA1 as a promising therapeutic target for bladder cancer. PMID: 31306103 [PubMed - as supplied by publisher]
Source: Cancer Biomarkers - July 16, 2019 Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research

Enhanced glycolytic metabolism supports transmigration of brain-infiltrating macrophages in multiple sclerosis
The migration of leukocytes into the CNS drives the neuropathology of multiple sclerosis (MS). It is likely that this penetration utilizes energy resources that remain to be defined. Using the experimental autoimmune encephalomyelitis (EAE) model of MS, we determined that macrophages within the perivascular cuff of postcapillary venules are highly glycolytic, as manifested by strong expression of lactate dehydrogenase A (LDHA), which converts pyruvate to lactate. These macrophages expressed prominent levels of monocarboxylate transporter-4 (MCT-4), which is specialized in the secretion of lactate from glycolytic cells. The...
Source: Journal of Clinical Investigation - July 16, 2019 Category: Biomedical Science Authors: Deepak Kumar Kaushik, Anindita Bhattacharya, Reza Mirzaei, Khalil S. Rawji, Younghee Ahn, Jong M. Rho, V. Wee Yong Source Type: research

gH625 cell-penetrating peptide promotes the endosomal escape of nanovectorized siRNA in a triple negative breast cancer cell line.
Abstract The use of small interfering RNA (siRNA) to regulate oncogenes appears as a promising strategy in the context of cancer therapy, especially if they are vectorized by a smart delivery system. In the present study, we investigated the cellular trafficking of a siRNA nanovector (called CS-MSN) functionalized with the cell-penetrating peptides (CPP) gH625 in a triple negative breast cancer model. With complementary techniques, we showed that siRNA nanovectors were internalized by both clathrin- and caveolae-mediated endocytosis. The presence of gH625 at the surface of the siRNA nanovector did not modify the e...
Source: Biomacromolecules - July 15, 2019 Category: Biochemistry Authors: Ben Djemaa S, Herve-Aubert K, Lajoie L, Falanga A, Galdiero S, Nedellec S, Soucé M, Munnier E, Chourpa I, David S, Allard-Vannier E Tags: Biomacromolecules Source Type: research

Molecules, Vol. 24, Pages 2570: Natural Polysaccharides for siRNA Delivery: Nanocarriers Based on Chitosan, Hyaluronic Acid, and Their Derivatives
Fuente Natural polysaccharides are frequently used in the design of drug delivery systems due to their biocompatibility, biodegradability, and low toxicity. Moreover, they are diverse in structure, size, and charge, and their chemical functional groups can be easily modified to match the needs of the final application and mode of administration. This review focuses on polysaccharidic nanocarriers based on chitosan and hyaluronic acid for small interfering RNA (siRNA) delivery, which are highly positively and negatively charged, respectively. The key properties, strengths, and drawbacks of each polysaccharide are discu...
Source: Molecules - July 15, 2019 Category: Chemistry Authors: In és Serrano-Sevilla Álvaro Artiga Scott G. Mitchell Laura De Matteis Jes ús M. de la Fuente Tags: Review Source Type: research

N6-methyl adenosine in siRNA evades immune response without reducing RNAi activity.
Authors: Imaeda A, Tomoike F, Hayakawa M, Nakamoto K, Kimura Y, Abe N, Abe H Abstract siRNA is a powerful method to suppress specific gene expression and has recently been utilized for molecular biology as well as medicine. However, introduction of dsRNA stimulates immune-responses as side-effects. In the present study, we utilized N6-methyl adenosine, one of the natural modified nucleosides, instead of adenosine in siRNA. When adenosine in the passenger or guide strand of siRNA was completely replaced with N6-methyl adenosine, the immune response against siRNA was evaded without any reduction in RNAi activity. Thi...
Source: Nucleosides, Nucleotides and Nucleic Acids - July 14, 2019 Category: Biochemistry Tags: Nucleosides Nucleotides Nucleic Acids Source Type: research

AAV-mediated siRNA against TRPV1 reduces nociception in a rat model of bone cancer pain.
Conclusions: Knockdown of TRPV1 effectively ameliorated mechanical allodynia and thermal hyperalgesia induced by TCI. Our data demonstrated that modulate the expression of TRPV1 in the spinal cord could be a potential therapeutic approach for bone cancer pain. PMID: 31296147 [PubMed - as supplied by publisher]
Source: Neurological Research - July 13, 2019 Category: Neurology Tags: Neurol Res Source Type: research

4'-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading.
Abstract We designed novel 4'-C-guanidinocarbohydrazidomethyl-5-methyl uridine (GMU) modified small interfering RNA (siRNA) and evaluated its biophysical and biochemical properties. Incorporation of GMU units significantly increased the thermodynamic stability as well as the enzymatic stability against nucleases in human serum. A gene silencing experiment indicated that GMU modfied siRNA (siRNA6) resulted in ≈4.9-fold more efficient knockdown than unmodified siRNA. PMID: 31298670 [PubMed - as supplied by publisher]
Source: Chemical Communications - July 12, 2019 Category: Chemistry Authors: Nawale GN, Bahadorikhalili S, Sengupta P, Kadekar S, Chatterjee S, Varghese OP Tags: Chem Commun (Camb) Source Type: research

Overcoming multiple drug resistance in lung cancer using siRNA targeted therapy.
Abstract Among cancers, lung cancer is the most morbidity and mortality disease that is remaining the fatalist. Generally, there are multiple treatment procedures for lung cancer, such as surgery, immunotherapy, radiotherapy and chemotherapy. There is, therefore, an urgent need for more specified and efficient methods for treatment of lung cancer such as RNAi, which in combination with traditional therapies could silence genes that are involved in the drug resistance. These genes may either be motivators of apoptosis inhibition, EMT and DNA repair system promoters or a member of intracellular signaling pathways, s...
Source: Gene - July 10, 2019 Category: Genetics & Stem Cells Authors: Naghizadeh S, Mohammadi A, Baradaran B, Mansoori B Tags: Gene Source Type: research

Targeting KRAS Mutant Lung Cancer Cells with siRNA-Loaded Bovine Serum Albumin Nanoparticles
ConclusionsBSA nanoparticles loaded with mutant specific siRNA are a promising therapeutic approach for KRAS-mutant cancers.
Source: Pharmaceutical Research - July 9, 2019 Category: Drugs & Pharmacology Source Type: research