Query: siRNA

This page shows you your search results in order of date.

Order by Relevance | Date

Total 18974 results found since Jan 2013.

Predisposition of Neonatal Maternal Separation to Visceral Hypersensitivity via Downregulation of Small-Conductance Calcium-Activated Potassium Channel Subtype 2 (SK2) in Mice.
Conclusions: ELS-induced visceral pain and visceral hypersensitivity are associated with the underfunction of SK2 channels in the spinal DH. PMID: 33029124 [PubMed - in process]
Source: Neural Plasticity - October 10, 2020 Category: Neurology Authors: Wu K, Gao JH, Hua R, Peng XH, Wang H, Zhang YM Tags: Neural Plast Source Type: research

Aptamer-Protamine-siRNA nanoparticles in targeted therapy of ErbB3 positive breast cancer cells
Publication date: Available online 9 October 2020Source: International Journal of PharmaceuticsAuthor(s): Xiangshang Xu, Li Li, Xiaolan Li, Deding Tao, Peng Zhang, Jianping Gong
Source: International Journal of Pharmaceutics - October 9, 2020 Category: Drugs & Pharmacology Source Type: research

Involvement of clock gene expression, bone morphogenetic protein and activin in adrenocortical steroidogenesis by human H295R cells.
Authors: Soejima Y, Iwata N, Nakano Y, Yamamoto K, Suyama A, Nada T, Ogawa H, Otsuka F Abstract Functional interactions between the levels of clock gene expression and adrenal steroidogenesis were studied in human adrenocortical H295R cells. Fluctuations of Bmal1, Clock, Per2 and Cry1 mRNA levels were found in H295R cells treated with forskolin (FSK) in a serum-free condition. The changes of clock gene expression levels were diverged, with Clock mRNA level being significantly higher than Cry1 and Per2 mRNA levels after 12-h stimulation with FSK. After FSK induction, mRNA levels of StAR and CYP11B2 were highest at 1...
Source: Endocrine Journal - October 9, 2020 Category: Endocrinology Tags: Endocr J Source Type: research

Down-regulation of Xist and Mir-7a-5p improves LPS-induced myocardial injury.
Conclusions: In this study, we established that down-regulation of xist and mir-7a-5p reduces apoptosis in response to LPS. PMID: 33029099 [PubMed - in process]
Source: International Journal of Medical Sciences - October 9, 2020 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Cancers, Vol. 12, Pages 2901: Dexamethasone Inhibits TRAIL-Induced Apoptosis through c-FLIP(L) Upregulation and DR5 Downregulation by GSK3 β Activation in Cancer Cells
This study was undertaken to investigate the effects of DEX on the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in cancer cells. We found that upregulation of c-FLIP(L) and downregulation of death receptor 5 (DR5; receptor for TRAIL ligand) contribute to the anti-apoptotic effect of DEX on TRAIL-induced apoptosis. DEX increased c-FLIP(L) expression at the transcriptional levels through the GSK-3β signaling pathway. The pharmacological inhibitor and catalytic mutant of GSK-3β suppressed DEX-induced upregulation of c-FLIP(L) expression. Furthermore, GSK-3&amp...
Source: Cancers - October 9, 2020 Category: Cancer & Oncology Authors: Mi-Yeon Jeon Seon Min Woo Seung Un Seo Sang Hyun Kim Ju-Ock Nam Shin Kim Jong-Wook Park Peter Kubatka Kyoung-jin Min Taeg Kyu Kwon Tags: Article Source Type: research

Molecules, Vol. 25, Pages 4591: Magnolol Enhances the Therapeutic Effects of TRAIL through DR5 Upregulation and Downregulation of c-FLIP and Mcl-1 Proteins in Cancer Cells
Kwon Magnolol is a biologically active compound, isolated from the Chinese herb Magnolia, that regulates antiproliferative, anticancer, antiangiogenic and antimetastatic activities. We found that magnolol sensitizes TRAIL-induced apoptotic cell death via upregulation of DR5 and downregulation of cellular FLICE-inhibitory protein (c-FLIP) and Mcl-1 in cancer cells, but not in normal cells. Mechanistically, magnolol increased ATF4-dependent DR5 expression at the transcription level, and knockdown of ATF4 markedly inhibited magnolol-induced DR5 upregulation. Silencing DR5 with siRNA prevented combined treatment with magn...
Source: Molecules - October 8, 2020 Category: Chemistry Authors: Seon Min Woo Kyoung-jin Min Taeg Kyu Kwon Tags: Article Source Type: research

Apoptosis in Cancer Cells Is Induced by Alternative Splicing of hnRNPA2/B1 Through Splicing of Bcl-x, a Mechanism that Can Be Stimulated by an Extract of the South African Medicinal Plant, Cotyledon orbiculata
Alternative splicing is deregulated in cancer and alternatively spliced products can be linked to cancer hallmarks. Targeting alternative splicing could offer novel effective cancer treatments. We investigated the effects of the crude extract of a South African medicinal plant, Cotyledon orbiculata, on cell survival of colon (HCT116) and esophageal (OE33 and KYSE70) cancer cell lines. Using RNASeq, we discovered that the extract interfered with mRNA regulatory pathways. The extract caused hnRNPA2B1 to splice from the hnRNPB1 to the hnRNPA2 isoform, resulting in a switch in the BCL2L1 gene from Bcl-xL to Bcl-xS causing acti...
Source: Frontiers in Oncology - October 8, 2020 Category: Cancer & Oncology Source Type: research

Abnormal expression of Rap2A as a prognostic marker for human breast cancer.
CONCLUSIONS: Rap2A acted as a promotor in the development of BC. Our findings suggested that Rap2A might be a new potential therapeutic target marker for BC treatment. PMID: 33015796 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - October 7, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

OTX1 is a novel regulator of proliferation, migration, invasion and apoptosis in lung adenocarcinoma.
CONCLUSIONS: Silencing the OTX1 gene suppressed the proliferation, migration and invasion of NCI-H292 and XWLC cells, impeded the cell cycle transition from G2 to M phase, and accelerated apoptosis, revealing OTX1, a regulator of NSCLC, as a potential new therapeutic target. PMID: 33015792 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - October 7, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Effect of lncRNA-BLACAT1 on drug resistance of non-small cell lung cancer cells in DDP chemotherapy by regulating cyclin D1 expression.
CONCLUSIONS: LncRNA-BLACAT1 regulates the expression of Cyclin D1, reduces the malignant phenotype of drug-resistant cells, and increases the sensitivity of lung cancer cells to DDP. PMID: 33015788 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - October 7, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Significance of TRPS1 in the development and clinicopathologic of hepatocellular carcinoma.
CONCLUSIONS: TRPS1 plays a key role in the development of HCC and is closely associated with the clinicopathological parameters of HCC patients. Hence, it is proposed that TRPS1 may serve as a novel prognostic marker and a potential therapeutic target for HCC treatment. PMID: 33015773 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - October 7, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Sirt7 protects chondrocytes degeneration in osteoarthritis via autophagy activation.
CONCLUSIONS: Overall, these findings suggested that Sirt7 was suppressed in the degenerated cartilage. Sirt7 deficiency does harm to the autophagy level, affecting CHs metabolism, while the upregulation of Sirt7 activated autophagy and protected CHs degeneration. An appropriate increase in autophagy can protect CHs but has no effect on Sirt7 expression. PMID: 33015765 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - October 7, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Zi Shen Huo Luo Formula Prevents Aldosterone-Induced Cardiomyocyte Hypertrophy and Cardiac Fibroblast Proliferation by Regulating the Striatin-Mediated MR/EGFR/ERK Signaling Pathway.
In conclusion, ZSHLF inhibits the downstream EGFR/ERK signaling pathway by blocking the striatin-mediated membrane localization of MR, which may be an important molecular mechanism by which ZSHLF improves aldosterone-induced myocardial remodeling. PMID: 33014117 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - October 7, 2020 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

Modulatory effect of aquaporin 5 on estrogen-induced epithelial-mesenchymal transition in prostate epithelial cells.
CONCLUSION: Our findings suggest that estrogen induces BPH possibly by promoting AQP5 expression. Hence, AQP5 might be a novel target for modulating EMT in prostate epithelial cells. PMID: 33031138 [PubMed - as supplied by publisher]
Source: Chinese Medical Journal - October 7, 2020 Category: General Medicine Authors: Fan Y, Song TR, Wei Q, Yang L, Lin T, Feng XB, Wang XD, Huang ZL, Feng SJ Tags: Chin Med J (Engl) Source Type: research

TMEM16A Mediated Mucus Production in Human Airway Epithelial Cells.
We examined this question using BCi-NS1 cells, a human airway basal cell line that maintains multipotent differentiation capacity, and the two human airway epithelial cell lines, Calu-3 and CFBE. The data demonstrate that exposure of airway epithelial cells to IL-8 and IL-13, two cytokines known to be enhanced in CF and asthma, respectively, leads to increase in mucus production. Expression of MUC5AC was fully dependent on expression of TMEM16A, as shown by siRNA-knockdown of TMEM16A. In addition, different inhibitors of TMEM16A attenuated IL-13 induced mucus production. Interestingly, in CFBE cells expressing F508delCFTR,...
Source: American Journal of Respiratory Cell and Molecular Biology - October 7, 2020 Category: Molecular Biology Authors: Cabrita I, Benedetto R, Wanitchakool P, Lerias J, Centeio R, Ousingsawat J, Schreiber R, Kunzelmann K Tags: Am J Respir Cell Mol Biol Source Type: research

MicroRNA-1297 downregulation inhibits breast cancer cell epithelial-mesenchymal transition and proliferation in a FA2H-dependent manner.
In conclusion, the present study indicated that miR-1297/FA2H might serve as a novel potential biomarker and therapeutic target for BC. PMID: 33014155 [PubMed]
Source: Oncology Letters - October 6, 2020 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Porphyric Neuropathy: Pathophysiology, Diagnosis, and Updated Management
AbstractPurpose of ReviewTo review the peripheral neurological complications of the acute hepatic porphyrias, as well as the latest advances in their pathophysiology and management.Recent FindingsThe diagnosis of porphyric neuropathy remains challenging as varying neuropathic patterns are encountered depending on disease stage, including a non-length-dependent distribution pattern. The major pathophysiologic mechanism is δ-aminolevulinic acid (ALA)–induced neurotoxicity. The less restrictive blood-nerve barrier in the autonomic ganglia and myenteric plexus may explain the frequency of dysautonomic manifestations. Recent...
Source: Current Neurology and Neuroscience Reports - October 6, 2020 Category: Neuroscience Source Type: research

The small molecule inhibitor PR-619 protects retinal ganglion cells against glutamate excitotoxicity
This study was designed to investigate the role of PR-619 in regulating mitophagy of RGCs under glutamate excitotoxicity. Primary cultured RGCs were incubated with PR-619 or vehicle control in the excitotoxicity model of 100 µM glutamate treatment. Mitochondrial membrane potential was assessed by JC-1 assay. Cytotoxicity of RGCs was measured by LDH activity. Proteins levels of parkin, optineurin, LAMP1, Bax, Bcl-2 and the LC3-II/I ratio were analyzed by western blot. The distribution and morphology of mitochondria in RGCs was stained by MitoTracker and antibody against mitochondria membrane protein, and examined by confoc...
Source: NeuroReport - October 6, 2020 Category: Neurology Tags: Degeneration and Repair Source Type: research

Distinct roles for Notch1 and Notch3 in human adipose-derived stem/stromal cell adipogenesis.
This study deepens the understanding of the Notch pathway by clarifying the distinct roles of Notch1 and Notch3 during adipogenesis. We showed that Notch3 is involved in early adipogenic differentiation, while Notch1 functions later in the process. In addition, we begin to uncover the interaction between the Notch and Wnt signaling pathways that may offer novel therapeutic targets aimed at obesity and diabetes. PMID: 33021719 [PubMed - as supplied by publisher]
Source: Molecular Biology Reports - October 5, 2020 Category: Molecular Biology Authors: Liu MC, Logan H, Newman JJ Tags: Mol Biol Rep Source Type: research

SCAP Mediated GDF15-Induced Invasion and EMT of Esophageal Cancer
Conclusions: We demonstrated that SCAP mediated GDF15-induced the invasion and metastasis of EC by regulating cholesterol metabolism. In addition, GDF15 was shown to be a direct target of miR-1324.
Source: Frontiers in Oncology - October 5, 2020 Category: Cancer & Oncology Source Type: research

Clinacanthus nutans Mitigates Neuronal Death and Reduces Ischemic Brain Injury: Role of NF- κB-driven IL-1β Transcription
AbstractNeuroinflammation has been shown to exacerbate ischemic brain injury, and is considered as a prime target for the development of stroke therapies.Clinacanthus nutans Lindau (C. nutans) is widely used in traditional medicine for treating insect bites, viral infection and cancer, due largely to its anti-oxidative and anti-inflammatory properties. Recently, we reported that an ethanol extract from the leaf ofC. nutans could protect the brain against ischemia-triggered neuronal death and infarction. In order to further understand the molecular mechanism(s) for its beneficial effects, two experimental paradigms, namely,...
Source: NeuroMolecular Medicine - October 5, 2020 Category: Neurology Source Type: research

Thrombospondin-2 stimulates MMP-9 production and promotes osteosarcoma metastasis via the PLC, PKC, c-Src and NF- κB activation.
Thrombospondin-2 stimulates MMP-9 production and promotes osteosarcoma metastasis via the PLC, PKC, c-Src and NF-κB activation. J Cell Mol Med. 2020 Oct 06;: Authors: Liu JF, Chen PC, Chang TM, Hou CH Abstract Osteosarcoma is an extremely common primary bone malignancy that is highly metastatic, with most deaths resulting from pulmonary metastases. The extracellular matrix protein thrombospondin-2 (TSP-2) is key to many biological processes, such as inflammation, wound repair and tissue remodelling. However, it is unclear as to what biological role TSP-2 plays in human metastatic osteosarcoma. The im...
Source: J Cell Mol Med - October 5, 2020 Category: Molecular Biology Authors: Liu JF, Chen PC, Chang TM, Hou CH Tags: J Cell Mol Med Source Type: research

AQP4-siRNA alleviates traumatic brain edema by altering post-traumatic AQP4 polarity reversal in TBI rats
Publication date: November 2020Source: Journal of Clinical Neuroscience, Volume 81Author(s): Hong Lu, Yuefu Zhan, Li Ai, Haixia Chen, Jianqiang Chen
Source: Journal of Clinical Neuroscience - October 5, 2020 Category: Neuroscience Source Type: research

Cloning short DNA into plasmids by one ‐step PCR
ConclusionsFree from the limitations of restriction enzyme sites and omitting the ligation process, our method offers a flexible and economical option of plasmid construction.Key pointsSignificant findings of the studyA method to clone short DNA into plasmids was found.What this study addsOur study provides a flexible and economical option to clone short DNA into plasmids.
Source: Thoracic Cancer - October 4, 2020 Category: Cancer & Oncology Authors: Cheng ‐Cheng Tao, Ying Yang, Fang Li, Ling Qiao, Yue Wu, Xiao‐Dong Sun, Yuan‐Yuan Zhang, Chang‐Long Li Tags: TECHNOLOGICAL NOTE Source Type: research

GSK-3 β mediates the effects of HNF-1β overexpression in ovarian clear cell carcinoma.
In conclusion and to the best of our knowledge, the current study was the first to determine that GSK-3β is a target gene of HNF-1β. In addition, the results of the present study revealed the novel HNF-1β-GSK-3β-p-NFκB pathway, occurring in response to DNA damage. Targeting this pathway may therefore represent a putative, novel, anticancer strategy in patients with OCCC. PMID: 33005248 [PubMed]
Source: Experimental and Therapeutic Medicine - October 4, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

Role of TLR4/MyD88/NF- κB signaling in the contrast-induced injury of renal tubular epithelial cells.
Role of TLR4/MyD88/NF-κB signaling in the contrast-induced injury of renal tubular epithelial cells. Exp Ther Med. 2020 Nov;20(5):115 Authors: Wang X, Zhou J, Yang J, Wang S, Yang L Abstract The aim of the present study was to explore the role of toll-like receptor 4 (TLR4)/myeloid differentiation primary response 88 (MyD88)/nuclear factor (NF)-κB signaling in the contrast-induced injury of renal tubular epithelial cells, and to investigate the potential mechanisms. HK-2 cells cultured in vitro were randomly divided into six groups as follows: i) The blank group; ii) the iohexol group; iii) the NF-κ...
Source: Experimental and Therapeutic Medicine - October 4, 2020 Category: General Medicine Tags: Exp Ther Med Source Type: research

M2 ‑TAM subsets altered by lactic acid promote T‑cell apoptosis through the PD‑L1/PD‑1 pathway.
M2‑TAM subsets altered by lactic acid promote T‑cell apoptosis through the PD‑L1/PD‑1 pathway. Oncol Rep. 2020 Nov;44(5):1885-1894 Authors: Shan T, Chen S, Chen X, Wu T, Yang Y, Li S, Ma J, Zhao J, Lin W, Li W, Cui X, Kang Y Abstract The aim of the study was to investigate the effects of lactic acid on the phenotypic polarization and immune function of macrophages. The human monocyte/macrophage cell line, THP‑1, was selected and treated with lactic acid. Immunofluorescence staining, laser confocal microscopy, reverse‑transcription polymerase chain reaction (RT‑PCR), western blot, siRNA, a...
Source: Oncology Reports - October 3, 2020 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

SPAG6 promotes cell proliferation and inhibits apoptosis through the PTEN/PI3K/AKT pathway in Burkitt lymphoma.
Authors: Zhang R, Zhu H, Yuan Y, Wang Y, Tian Z Abstract The main purpose of the present study was to elucidate the role of sperm‑associated antigen 6 (SPAG6) in the occurrence and development of Burkitt lymphoma (BL) and explore the underlying molecular mechanisms. A correlation was observed between the expression of SPAG6 and the prognosis of patients with lymphoma using The Cancer Genome Atlas (TCGA) database analysis. It was demonstrated that the levels of SPAG6 in BL cells were higher compared with that in IM‑9 cells by reverse transcription‑PCR and western blot assays. Moreover, silencing of SPAG6 sig...
Source: Oncology Reports - October 3, 2020 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Pharmacologic Inhibition of Ezrin-Radixin-Moesin Phosphorylation is a Novel Therapeutic Strategy in Rhabdomyosarcoma.
This study investigates the inhibition of ERM phosphorylation using a small molecule pharmacophore NSC668394 as a potential strategy against RMS. Upon in vitro treatment with NSC668394, RMS cells exhibit a dose-dependent decrease in cell viability and proliferation, with induction of caspase-3 cleavage and apoptosis. siRNA-mediated knockdown of individual ERM protein expression revealed that each regulates RMS survival to a different degree. In vivo administration of NSC668394 in RMS xenografts causes significant decrease in tumor growth, with no adverse effect on body weight. Collectively, this study highlights the import...
Source: Sarcoma - October 3, 2020 Category: Cancer & Oncology Tags: Sarcoma Source Type: research

Protective effects of valsartan administration on doxorubicin ‑induced myocardial injury in rats and the role of oxidative stress and NOX2/NOX4 signaling.
Protective effects of valsartan administration on doxorubicin‑induced myocardial injury in rats and the role of oxidative stress and NOX2/NOX4 signaling. Mol Med Rep. 2020 Nov;22(5):4151-4162 Authors: Cheng D, Chen L, Tu W, Wang H, Wang Q, Meng L, Li Z, Yu Q Abstract Clinical application of doxorubicin (DOX) is hampered by its potential cardiotoxicity, however angiotensin receptor blockers could attenuate DOX‑induced cardiomyopathy. The present study tested the hypothesis that simultaneous administration of valsartan (Val) with DOX could prevent DOX‑induced myocardial injury by modulating myoca...
Source: Molecular Medicine Reports - October 3, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Neuropeptide  Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism.
Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism. Mol Med Rep. 2020 Nov;22(5):4376-4382 Authors: Zhang B, Zhang X, Xiao J, Zhou X, Chen Y, Gao C Abstract The neuropeptide Y (NPY) system is considered one of the primary neural signaling pathways. NPY, produced by osteoblasts and other peripheral tissues, is known to inhibit biological functions of osteoblasts. However, until recently, little was known of the autocrine mechanism by which NPY is regulated. To investigate this mechanism, overexpression plasmids and small interferi...
Source: Molecular Medicine Reports - October 3, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Effects of PEG anchors in PEGylated siRNA lipoplexes on in  vitro gene‑silencing effects and siRNA biodistribution in mice.
Effects of PEG anchors in PEGylated siRNA lipoplexes on in vitro gene‑silencing effects and siRNA biodistribution in mice. Mol Med Rep. 2020 Nov;22(5):4183-4196 Authors: Hattori Y, Tamaki K, Sakasai S, Ozaki KI, Onishi H Abstract Polyethylene glycol (PEG)‑modifications (PEGylations) of cationic liposome/small interfering RNA complexes (siRNA lipoplexes) can enhance their systemic stability. The present study determined the effects of PEG anchors in PEGylated siRNA lipoplexes on in vitro gene‑silencing effects and siRNA biodistribution after intravenous injection. Three types of dialkyl or trial...
Source: Molecular Medicine Reports - October 3, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

USP7 targeting modulates anti-tumor immune response by reprogramming Tumor-associated Macrophages in Lung Cancer
Conclusions: Taken together, these results provide evidence to suggest that therapeutic approaches targeting USP7, in combination with immunotherapy, should be considered for lung cancer treatment.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Xiaomeng Dai, Lisen Lu, Suke Deng, Jingshu Meng, Chao Wan, Jing Huang, Yajie Sun, Yan Hu, Bian Wu, Gang Wu, Jonathan F. Lovell, Honglin Jin, Kunyu Yang Tags: Research Paper Source Type: research

Cardiomyocyte-derived small extracellular vesicles can signal eNOS activation in cardiac microvascular endothelial cells to protect against Ischemia/Reperfusion injury
Conclusions: These data uncover a mechanism by which the crosstalk between CMs and CMECs leads to the increased survival of the heart after I/R injury and point to a new therapeutic target for the blunting of myocardial I/R injury.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Guihao Chen, Chuansheng Xu, Thomas G. Gillette, Tongyi Huang, Peisen Huang, Qing Li, Xiangdong Li, Qinfeng Li, Yu Ning, Ruijie Tang, Cunrong Huang, Yuyan Xiong, Xiaqiu Tian, Jun Xu, Junyan Xu, Liping Chang, Cong Wei, Chen Jin, Joseph A. Hill, Yuejin Yang Tags: Research Paper Source Type: research

Nanoscale microenvironment engineering based on layer-by-layer self-assembly to regulate hair follicle stem cell fate for regenerative medicine
Conclusion: This study provides novel methods for the construction of stem cell microenvironments and experimental models of HF regeneration for the treatment of hair loss.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Peng Chen, Yong Miao, Feifei Zhang, Junfei Huang, Yuxin Chen, Zhexiang Fan, Lunan Yang, Jin Wang, Zhiqi Hu Tags: Research Paper Source Type: research

CXCR4 induces cell autophagy and maintains EBV latent infection in EBVaGC
Conclusions: Our data suggested new roles for CXCR4 in autophagy and EBV replication in EBVaGC, which further promoted cell survival and persistent latent infection. These new findings can lead to further CXCR4-based anticancer therapy.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Weiwen Wang, Yan Zhang, Wen Liu, Xiangyan Zhang, Hua Xiao, Menghe Zhao, Bing Luo Tags: Research Paper Source Type: research

Increased intracellular Cl- concentration improves airway epithelial migration by activating the RhoA/ROCK Pathway
Conclusion: Taken together, these results suggested that Cl- accumulation in airway epithelial cells could activate the RhoA/ROCK/LIMK cascade to induce F-actin reorganization, down-regulate cell stiffness, and improve epithelial migration.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Wenjie Huang, Meiling Tan, Yue Wang, Lei Liu, Yan Pan, Jingjing Li, Mingxing Ouyang, Chunjiao Long, Xiangping Qu, Huijun Liu, Chi Liu, Jia Wang, Linhong Deng, Yang Xiang, Xiaoqun Qin Tags: Research Paper Source Type: research

pH-responsive DNA nanomicelles for chemo-gene synergetic therapy of anaplastic large cell lymphoma
Conclusion: DNMs co-loaded with Dox and ALK-specific siRNA exhibited significantly enhanced apoptosis of ALCL K299 cells in vitro and effectively inhibited tumor growth in vivo without obvious toxicity, providing a potential strategy in the development of nanomedicines for synergetic cancer therapy.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Yuwei Li, Shuzhen Yue, Jingyu Cao, Chengzhan Zhu, Yixiu Wang, Xin Hai, Weiling Song, Sai Bi Tags: Research Paper Source Type: research

Flubendazole elicits anti-cancer effects via targeting EVA1A-modulated autophagy and apoptosis in Triple-negative Breast Cancer
Conclusion: Our results provide novel insights towards the putative anti-cancer efficacy of flubendazole. Furthermore, here we show that flubendazole could serve as a potential therapeutic drug in TNBC. Altogether, this study highlights the possibility of this repurposed autophagic inducer for future cancer treatments.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Yongqi Zhen, Rongyan Zhao, Minjuan Wang, Xing Jiang, Feng Gao, Leilei Fu, Lan Zhang, Xian-Li Zhou Tags: Research Paper Source Type: research

circ-PRKCB acts as a ceRNA to regulate p66Shc-mediated oxidative stress in intestinal ischemia/reperfusion
Conclusions: Our findings reveal a crucial role for the circ-PRKCB/miR-339-5p/p66Shc signaling pathway in regulating oxidative stress in the I/R intestine. This pathway may be a potential therapeutic target for intestinal I/R injury.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Dongcheng Feng, Zhecheng Wang, Yan Zhao, Yang Li, Deshun Liu, Zhao Chen, Shili Ning, Yan Hu, Jihong Yao, Xiaofeng Tian Tags: Research Paper Source Type: research

Cadherin-11 cooperates with inflammatory factors to promote the migration and invasion of fibroblast-like synoviocytes in pigmented villonodular synovitis
Conclusions: Cadherin-11 plays an important role in the pathogenesis of PVNS and forms a positive feedback loop with inflammatory factors, which further activates the NF-κB and MAPK pathways to trigger an inflammatory cascade. Cadherin-11-mediated inflammation results in PVNS with high recurrence, invasiveness, and strong cartilage destruction ability, and eventually promotes the transformation of PVNS from the initial inflammatory disease to neoplastic disease. Thus, inhibition of cadherin-11 together with its related inflammatory reaction, represents a new therapeutic strategy for PVNS.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Chenxi Cao, Fei Wu, Xingyue Niu, Xiaoqing Hu, Jin Cheng, Yan Zhang, Chuanyun Li, Xiaoning Duan, Xin Fu, Jiying Zhang, Xin Zhang, Yingfang Ao Tags: Research Paper Source Type: research

Low-intensity Pulsed Ultrasound regulates alveolar bone homeostasis in experimental Periodontitis by diminishing Oxidative Stress
Conclusions: These results demonstrated that LIPUS regulates alveolar bone homeostasis in periodontitis by attenuating oxidative stress via the regulation of PI3K-Akt/Nrf2 signaling. Thus, Nrf2 plays a pivotal role in the protective effect exerted by LIPUS against ligature-induced experimental periodontitis.
Source: Theranostics - October 3, 2020 Category: Molecular Biology Authors: Siqi Ying, Minmin Tan, Ge Feng, Yunchun Kuang, Duanjing Chen, Jie Li, Jinlin Song Tags: Research Paper Source Type: research

Role of ARID1A in epithelial ‑mesenchymal transition in breast cancer and its effect on cell sensitivity to 5‑FU.
Role of ARID1A in epithelial‑mesenchymal transition in breast cancer and its effect on cell sensitivity to 5‑FU. Int J Mol Med. 2020 Nov;46(5):1683-1694 Authors: Wang T, Gao X, Zhou K, Jiang T, Gao S, Liu P, Zuo X, Shi X Abstract The loss of function mutation of AT‑rich interactive domain 1A (ARID1A) often occurs in patients with breast cancer. It has been found that ARID1A knockout can enhance both the migratory activity of renal carcinoma cells and their sensitivity to therapeutic drugs by promoting epithelial-mesenchymal transition (EMT); however, its mechanisms of action in breast cancer r...
Source: International Journal of Molecular Medicine - October 3, 2020 Category: Molecular Biology Authors: Wang T, Gao X, Zhou K, Jiang T, Gao S, Liu P, Zuo X, Shi X Tags: Int J Mol Med Source Type: research

Loss of lncRNA MIAT ameliorates proliferation and fibrosis of diabetic nephropathy through reducing E2F3 expression.
In this study, we tested expression level of MIAT in DN patients and mesangial cells treated by high glucose (HG). Up-regulation of MIAT was observed in DN. Then, functional assays displayed that silence of MIAT by siRNA significantly repressed the proliferation and cycle progression in mesangial cells induced by HG. Meanwhile, we found that collagen IV, fibronectin and TGF-β1 protein expression was obviously triggered by HG, which could be rescued by loss of MIAT. Then, further assessment indicated that MIAT served as sponge harbouring miR-147a. Moreover, miR-147a was decreased in DN, which exhibited an antagonistic effe...
Source: J Cell Mol Med - October 2, 2020 Category: Molecular Biology Authors: Ji TT, Qi YH, Li XY, Tang B, Wang YK, Zheng PX, Li W, Qu X, Feng L, Bai SJ Tags: J Cell Mol Med Source Type: research

Cancers, Vol. 12, Pages 2857: The Oncoprotein SKI Acts as A Suppressor of NK Cell-Mediated Immunosurveillance in PDAC
on Strandmann Drugs targeting epigenetic mechanisms such as histone deacetylase inhibitors (HDACi) suppress tumor growth. HDACi also induce the expression of ligands for the cytotoxicity receptor NKG2D rendering tumors more susceptible to natural killer (NK) cell-dependent killing. The major acetylases responsible for the expression of NKG2D ligands (NKG2D-L) are CBP and p300. The role of the oncogene and transcriptional repressor SKI, an essential part of an HDAC-recruiting co-repressor complex, which competes with CBP/p300 for binding to SMAD3 in TGFβ signaling, is unknown. Here we show that the siRNA-me...
Source: Cancers - October 2, 2020 Category: Cancer & Oncology Authors: Viviane Ponath Miriam Frech Mathis Bittermann Reem Al Khayer Andreas Neubauer Cornelia Brendel Elke Pogge von Strandmann Tags: Article Source Type: research

Lyophilization and nebulization of pulmonary surfactant-coated nanogels for siRNA inhalation therapy.
Abstract RNA interference (RNAi) enables highly specific silencing of potential target genes for treatment of pulmonary pathologies. The intracellular RNAi pathway can be activated by cytosolic delivery of small interfering RNA (siRNA), inducing sequence-specific gene knockdown on the post-transcriptional level. Although siRNA drugs hold many advantages over currently applied therapies, their clinical translation is hampered by inefficient delivery across cellular membranes. We previously developed hybrid nanoparticles consisting of an siRNA-loaded nanosized hydrogel core (nanogel) coated with Curosurf®, a clinic...
Source: European Journal of Pharmaceutics and Biopharmaceutics - October 2, 2020 Category: Drugs & Pharmacology Authors: Merckx P, Lammens J, Nuytten G, Bogaert B, Guagliardo R, Maes T, Vervaet C, De Beer T, De Smedt SC, Raemdonck K Tags: Eur J Pharm Biopharm Source Type: research

Chemical Approaches for RNAi Drug Development.
This article focuses on our two original approaches. The first involves the groove modification of siRNA duplexes to understand siRNA-protein interactions using 7-bromo-7-deazaadenosine and 3-bromo-3-deazaadenosine as chemical probes, while the second involves the generation of RNAi medicine using chemically modified DNA, known as an intelligent shRNA expression device (iRed). PMID: 32999205 [PubMed - in process]
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - October 2, 2020 Category: Drugs & Pharmacology Authors: Saito-Tarashima N Tags: Yakugaku Zasshi Source Type: research

Patient-specific genomics and cross-species functional analysis implicate LRP2 in hypoplastic left heart syndrome
Congenital heart diseases (CHDs), including hypoplastic left heart syndrome (HLHS), are genetically complex and poorly understood. Here, a multi-disciplinary platform was established to functionally evaluate novel CHD gene candidates, based on whole genome and iPSC RNA sequencing of a HLHS family-trio. Filtering for rare variants and altered expression in proband iPSCs prioritized 10 candidates. siRNA/RNAi-mediated knockdown in generic human iPSC-derived cardiomyocytes (hiPSC-CM) and in developingDrosophilaand zebrafish hearts revealed that LDL receptor-related proteinLRP2 is required for cardiomyocyte proliferation and di...
Source: eLife - October 2, 2020 Category: Biomedical Science Tags: Genetics and Genomics Medicine Source Type: research

LINC00294 induced by GRP78 promotes cervical cancer development by promoting cell cycle transition.
Authors: Qiu J, Zhou S, Cheng W, Luo C Abstract Cervical cancer is one of the most common gynecological malignancies, and it has become a crucial public health problem. In the present study, the expression profiles of cervical cancer and normal cervical tissues were downloaded from the Gene Expression Omnibus and The Cancer Genome Atlas databases. Subsequently, the dysregulated long non-coding RNAs (lncRNAs) in cervical cancer were identified using R software Differentially expressed lncRNAs in cervical cancer that were associated with glucose-regulated protein 78 (GRP78) were screened out and the results demonstra...
Source: Oncology Letters - October 1, 2020 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Contrasting activities of estrogen receptor beta isoforms in triple negative breast cancer
ConclusionsER β2/ERβ5 and ERβ1 exhibit sharply contrasting activities in TNBC cells. Our findings imply that delineating the absolute amounts and relative ratios of the different ERβ isoforms might have prognostic and therapeutic relevance, and could enable better selection of optimal approaches for treatment of this often aggressive form of breast cancer.
Source: Breast Cancer Research and Treatment - September 30, 2020 Category: Cancer & Oncology Source Type: research