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Total 40893 results found since Jan 2013.
ABCA siRNA Transfection of Neurons & Astrocytes
In this study, Dr. Marina Guizzetti and his team use our i-Fect Kit and several other commercially available kits to knockdown ABCG1 and ABCG4: Jing Chen, Xiaolu Zhang, Handojo Kusumo, Lucio G. Costa, c, Marina Guizzettia. Cholesterol efflux is differentially regulated in neurons and astrocytes: Implications for brain cholesterol homeostasis. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1831 (2), p.263-275, Feb 2013. doi:10.1016/j.bbalip.2012.09.007. Abstract: Disruption of cholesterol homeostasis in the central nervous system (CNS) has been associated with neurological, neurodegenerative...
Source: siRNA and DsiRNA Transfection Efficiency - November 17, 2012 Category: Neuroscience Tags: ABCA1 i-Fect siRNA Delivery in vitro siRNA Transfection Efficiency siRNA transfection of neurons siRNA trasfection of astrocytes Source Type: news
i-Fect ™ Delivers you siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence K V Receptors. Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons . In these 2 latest expamples, researchers use i-Fect to deliver siRNA to K V Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570 –579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal...
Source: siRNA and DsiRNA Transfection Efficiency - March 16, 2013 Category: Neuroscience Tags: Delivering siRNA to the CNS Dorsal root ganglia i-Fect in vivo siRNA intrathecal delivery of siRNA KV Receptors kv1.1 Potassium Channel Subunit Kv9.1 Potassium Channel Subunit Transfection Kits Source Type: news
i-Fect™ Delivers you siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence KV Receptors.Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons. In these 2 latest expamples, researchers use i-Fect to deliver siRNA to KV Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570–579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal treatment...
Source: siRNA and DsiRNA Transfection Efficiency - March 16, 2013 Category: Neuroscience Tags: Kv9.1 Potassium Channel Subunit kv1.1 Potassium Channel Subunit Delivering siRNA to the CNS Transfection Kits KV Receptors i-Fect Dorsal root ganglia intrathecal delivery of siRNA in vivo siRNA Source Type: news
i-Fect Delivers IGF1 siRNA to the Mouse Brain
Intraventricular Injections Used to Study Neuronal Survival During Post Natal DevelopmentNeuromics' i-FectTM Transfection Kit continue to be successfully used to deliver siRNA, shRNA and miRNA to cell cultures and the CNS in vivo (via intrathecal, epidural and intraventricular injection). Genes studied include: ABCA, ASIC, β-arrestin, CAV1.2, IGF1, CX3CR1, DOR, ELOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 ,The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK,SNSR1, hTertTRPV1 NOV, Survivin, TLR4, Troy and TRPV1. Related Publications.It is always an honor for one our products to be referenced in one of the...
Source: siRNA and DsiRNA Transfection Efficiency - April 25, 2013 Category: Neuroscience Tags: siRNA delivery in-vivo siRNA delivery to mouse brain i-Fect Gene Silencing intraventicular injections IGF1 gene expression in vivo siRNA Source Type: news
i-Fect siRNA Transfection Successes
Meeting your Gene Expression Analysis Requirements.I have posted many success stories resulting from use of Neuromics' Neuromics' i-FectTM siRNA Transfection Kit. It has been 3 months since my last posting so here I would like to post some recent highlights. Here I feature customer publication demonstrating:Down regulation of Neuroligin 2-data showed unexpected upregulation and pronociceptive effects of the “inhibitory” NL2 in neuropathic pain, suggesting a functional shift of NL2 from inhibition to excitation that changed the synaptic ratio toward higher excitation.Role of IGFPB5 for survival of neurons in...
Source: siRNA and DsiRNA Transfection Efficiency - July 29, 2013 Category: Neuroscience Tags: Neuroligin 2 Gene berrant Histone Deacetylase2 gene IGFBP5 gene NL2 intraventicular delivery of siRNA ADAC2 i-Fect PSD95 intrathecal delivery of siRNA in vivo siRNA Source Type: news
i-Fect Delivers siRNA to Study Angiogenesis in Brain Injury
This study revealed that LDHA, which is a known damage marker, promotes CNS-specific angiogenesis. LDHA-mediated angiogenesis depends on vimentin on the surface of vascular endothelial cells. The work described here proposes a novel mechanism by which neurodegeneration drives angiogenesis in the CNS.A mixture of ouri-FectTM and LDHA siRNA, in this study, were directly injected into mice cortexes: Hsiaoyun Lin, Rieko Muramatsu, Noriko Maedera, Hiroto Tsunematsu, Machika Hamaguchi, Yoshihisa Koyama, Mariko Kuroda, Kenji Ono, Makoto Sawada, Toshihide Yamashita.Extracellular Lactate Dehydrogenase A Release ...
Source: siRNA and DsiRNA Transfection Efficiency - February 19, 2018 Category: Neuroscience Tags: Angiogenesis Delivering siRNA to the CNS i-Fect Lactate Dehydrogenase A LDHA siRNA delivery to mouse brain Source Type: news
Delivery of siRNA, miRNA, shRNA and Plasmids Guaranteed
Potent and Frequently Published Transfection Solutions We have proven and frequently published transfection solutions. Powerful punch and versatility are now needed more than ever with the hyper growth in gene manipulation technologies like Sleeping BeautyTM and CRISPR-Cas9.We have published examples of the use of our solutions for delivering siRNA, miRNA, shRNA and plasmids both in vitro and in vivo. Here are some of your options. i-Fect ™ -A novel cationic lipid formulation specifically designed for efficient delivery of 27mer DsiRNAs(dicer substrate small Interfering RNAs)& 21mer siRNAs (small interfering RNA...
Source: siRNA and DsiRNA Transfection Efficiency - January 20, 2016 Category: Neuroscience Tags: DNA transfection i-Fect miRNA Delivery Methods p-fect Plasmid Transfection plasmids pn-Fect shRNA siRNA Source Type: news
Delivering siRNA in vivo
New Publications Ouri-Fect transfection kit was one of the first products. This has resulted in it being widely used andfrequently published.We kick off 2018 with some new publications. Here we feature a study where researchers use our kit to deliver G-protein-coupled receptor C (MrgprC) siRNA in vivo. This receptor is part of the TRPV1 pathway.Ying-jun Liu, Xiao-xi Lin, Jian-qiao Fang, and Fang Fang. Involvement of MrgprC in electroacupuncture analgesia for attenuating CFA-induced thermal hyperalgesia by suppressing the TRPV1 pathway. Pre-pub-full text PDFImage: Expression of TRPV1 in rats treated with MrgrprC s...
Source: siRNA and DsiRNA Transfection Efficiency - January 13, 2018 Category: Neuroscience Tags: inflammatory pain intrathecal delivery of siRNA Mas-related G-protein-coupled receptor C MrgrpfC TRPV1 Source Type: news
High Fat and Diet Induced Obesity
i-FectTM Delivers Again!Research shows that rats and humans on a high-fat diet (HFD) are less sensitive to satiety signals known to act via vagal afferent pathways. Impaired vagal afferent responsiveness to both gastric satiety hormones (CCK and leptin) and mechanical stimulation raises the possibility that changes in electrophysiological properties may be the underlying mechanism responsible for impaired vagal responsiveness to a wide variety of satiety signals.Potassium channels play a central role. To demonstrate this researchers used ouri-Fect siRNA Transfection Kit to silence TRESK and TASK1 to understand their impact...
Source: siRNA and DsiRNA Transfection Efficiency - September 16, 2019 Category: Neuroscience Tags: iFect in vivo siRNA intrathecal delivery of siRNA RNAi TRESK Source Type: news
i-Fect™ Delivers Your siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence KV Receptors.Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons. In these 2 latest expamples, researchers use i-Fect to deliver siRNA to KV Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570–579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal treatment...
Source: Neuromics - March 16, 2013 Category: Neuroscience Tags: siRNA delivery in-vivo Delivering siRNA to the CNS KV receptors. i-Fect Kv9.1 Potassium Channel Source Type: news
Epigenetics and Pain Research
i-Fect Used to Study ImpactsOur i-Fect siRNA, miRNA and shRNA Trasfection Kit was recently used to study the impact of G9a-specific siRNA (AGUAACGGGCAUCAAUGC) on Mu Opioid Receptors: Yuhao Zhang, Shao-Rui Chen, Geoffroy Laumet, Hong Chen and Hui-Lin Pan. Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-Mediated Transcriptional Repression of µ-Opioid Receptors in Primary Sensory Neurons. First Published on February 25, 2016, doi: 10.1074/jbc.M115.711812... In some SNL rats, G9a-specific siRNA (4 µg) or the negative control siRNA was administered intrathecally. G9a-specific siRNA(AGUAACGGGCAUC...
Source: siRNA and DsiRNA Transfection Efficiency - March 24, 2016 Category: Neuroscience Tags: epigenetics Ga9 Mu Opioid Receptor inhibitor i-Fect iFect intrathecal delivery of siRNA Morphine neuropathic pain Source Type: news
Silencing Cytokines in-vivo with i-Fect
Knocking Down Cytokine to Study Pain ResponseWe have many unique applications published from researchers using our Transfection Kits in vitro and in vivo. Here researchers simultaneously 3 immune/inflammatory response cytokines in vivo: Byung Moon Choi, Soo Han Lee, Sang Mee An, Do Yang Park, Gwan Woo Lee, and Gyu-Jeong Noh. The time-course and RNA interference of TNF-α, IL-6, and IL-1β expression on neuropathic pain induced by L5 spinal nerve transection in rats. Korean J Anesthesiol. 2015 Apr;68(2):159-169. English. Published online March 30, 2015. http://dx.doi.org/10.4097/kjae.2015.68.2.159.Protocol: RNAs w...
Source: siRNA and DsiRNA Transfection Efficiency - April 7, 2015 Category: Neuroscience Tags: allodynia analgesia Gene Expression Analysis Gene Silencing i-Fect IL-1B IL-6 inflammatory pain intrathecal delivery of siRNA TNF-alpha Transfection Kits Source Type: news
Gene Expression Analysis For Neuroscientists
In Vitro and In Vivo StudiesNeuromics has a strong line up of Transfection Tools designed specifically for Neuroscientists. Neurons, Glia and Astrocytes are notoriously hard to transfect. We are proud of our track record.This is an excellent study for Neuroscience Researchers interested in using best methods: http://emboj.embopress.org/content/embojnl/31/15/3239/DC1/embed/inline-supplementary-material-1.pdf?download=true. Here researchers delivered 14-3-3 siRNA sub units + i-Fect intrathecally. Here's specific knockdown results:Figure: A. Dose-dependent inhibition of 14-3-3-zeta (z) expression with ant...
Source: siRNA and DsiRNA Transfection Efficiency - June 25, 2014 Category: Neuroscience Tags: 14-3-3 zeta i-Fect in vivo RNAi intrathecal delivery of siRNA intravenous delivery of siRNA siRNA in vivo Transfection Source Type: news
i-Fect in Action
This study is the first to demonstrate that the stabilization of HIF1A expression underpins the development of bortezomib-induced neuropathic pain. Crucially, these findings reveal that the initiation and maintenance of bortezomib-induced neuropathic pain are regulated by distinct mechanisms.Looking to up your odds for high percentage siRNA Transfection? Try i-Fect.
Source: siRNA and DsiRNA Transfection Efficiency - May 9, 2019 Category: Neuroscience Tags: Chemotherapy i-Fect in vivo RNAi neuropathic pain siRNA delivery in-vivo Source Type: news
Small molecule ligands for enhanced intracellular delivery of lipid nanoparticle formulations of siRNA
This study aimed to identify small molecules that enhance cellular uptake of LNP siRNA systems, then use them as LNP-associated ligands to improve gene silencing potency. Screening the Canadian Chemical Biology Network molecules for effects on LNP uptake into HeLa cells found that cardiac glycosides like ouabain and strophanthidin caused the highest uptake. Cardiac glycosides stimulate endocytosis on binding to plasma membrane Na+/K+ ATPase found in all mammalian cells, offering the potential to stimulate LNP uptake into various cell types. A PEG-lipid containing strophanthidin at the end of PEG (STR-PEG-lipid) was synthes...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - December 10, 2012 Category: Nanotechnology Authors: Yuen Yi C. Tam, Sam Chen, Josh Zaifman, Ying K. Tam, Paulo J.C. Lin, Steven Ansell, Michel Roberge, Marco A. Ciufolini, Pieter R. Cullis Tags: Pharmacology, Pharmaceutics, Drug Delivery, siRNA, Lipid NPs, Cardiac Glycosides Source Type: research
