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Total 13806 results found since Jan 2013.

i-Fect™ Delivers you siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence KV Receptors.Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons. In these 2 latest expamples, researchers use i-Fect to deliver siRNA to KV Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570–579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal treatment...
Source: siRNA and DsiRNA Transfection Efficiency - March 16, 2013 Category: Neuroscience Tags: Kv9.1 Potassium Channel Subunit kv1.1 Potassium Channel Subunit Delivering siRNA to the CNS Transfection Kits KV Receptors i-Fect Dorsal root ganglia intrathecal delivery of siRNA in vivo siRNA Source Type: news

i-Fect Delivers IGF1 siRNA to the Mouse Brain
Intraventricular Injections Used to Study Neuronal Survival During Post Natal DevelopmentNeuromics' i-FectTM Transfection Kit continue to be successfully used to deliver siRNA, shRNA and miRNA to cell cultures and the CNS in vivo (via intrathecal, epidural and intraventricular injection). Genes studied include: ABCA, ASIC, β-arrestin, CAV1.2, IGF1, CX3CR1, DOR, ELOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 ,The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK,SNSR1, hTertTRPV1 NOV, Survivin, TLR4, Troy and TRPV1. Related Publications.It is always an honor for one our products to be referenced in one of the...
Source: siRNA and DsiRNA Transfection Efficiency - April 25, 2013 Category: Neuroscience Tags: siRNA delivery in-vivo siRNA delivery to mouse brain i-Fect Gene Silencing intraventicular injections IGF1 gene expression in vivo siRNA Source Type: news

i-Fect siRNA Transfection Successes
Meeting your Gene Expression Analysis Requirements.I have posted many success stories resulting from use of  Neuromics'  Neuromics' i-FectTM siRNA Transfection Kit. It has been 3 months since my last posting so here I would like to post some recent highlights. Here I feature customer publication demonstrating:Down regulation of Neuroligin 2-data showed unexpected upregulation and pronociceptive effects of the “inhibitory” NL2 in neuropathic pain, suggesting a functional shift of NL2 from inhibition to excitation that changed the synaptic ratio toward higher excitation.Role of IGFPB5 for survival of neurons in...
Source: siRNA and DsiRNA Transfection Efficiency - July 29, 2013 Category: Neuroscience Tags: Neuroligin 2 Gene berrant Histone Deacetylase2 gene IGFBP5 gene NL2 intraventicular delivery of siRNA ADAC2 i-Fect PSD95 intrathecal delivery of siRNA in vivo siRNA Source Type: news

Delivery of siRNA, miRNA, shRNA and Plasmids Guaranteed
Potent and Frequently Published Transfection Solutions We have proven and frequently published transfection solutions. Powerful punch and versatility are now needed more than ever with the hyper growth in gene manipulation technologies like Sleeping BeautyTM and CRISPR-Cas9.We have published examples of the use of our solutions for delivering siRNA, miRNA, shRNA and plasmids both in vitro and in vivo. Here are some of your options. i-Fect ™ -A novel cationic lipid formulation specifically designed for efficient delivery of 27mer DsiRNAs(dicer substrate small Interfering RNAs)& 21mer siRNAs (small interfering RNA...
Source: siRNA and DsiRNA Transfection Efficiency - January 20, 2016 Category: Neuroscience Tags: DNA transfection i-Fect miRNA Delivery Methods p-fect Plasmid Transfection plasmids pn-Fect shRNA siRNA Source Type: news

i-Fect ™ Delivers you siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence K V Receptors. Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons . In these 2 latest expamples, researchers use i-Fect to deliver siRNA to K V Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570 –579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal...
Source: siRNA and DsiRNA Transfection Efficiency - March 16, 2013 Category: Neuroscience Tags: Delivering siRNA to the CNS Dorsal root ganglia i-Fect in vivo siRNA intrathecal delivery of siRNA KV Receptors kv1.1 Potassium Channel Subunit Kv9.1 Potassium Channel Subunit Transfection Kits Source Type: news

ABCA siRNA Transfection of Neurons & Astrocytes
In this study, Dr. Marina Guizzetti and his team use our i-Fect Kit and several other commercially available kits to knockdown ABCG1 and ABCG4: Jing Chen, Xiaolu Zhang, Handojo Kusumo, Lucio G. Costa, c, Marina Guizzettia. Cholesterol efflux is differentially regulated in neurons and astrocytes: Implications for brain cholesterol homeostasis. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1831 (2), p.263-275, Feb 2013. doi:10.1016/j.bbalip.2012.09.007. Abstract: Disruption of cholesterol homeostasis in the central nervous system (CNS) has been associated with neurological, neurodegenerative...
Source: siRNA and DsiRNA Transfection Efficiency - November 17, 2012 Category: Neuroscience Tags: ABCA1 i-Fect siRNA Delivery in vitro siRNA Transfection Efficiency siRNA transfection of neurons siRNA trasfection of astrocytes Source Type: news

Delivering siRNA in vivo
New Publications Ouri-Fect transfection kit was one of the first products. This has resulted in it being widely used andfrequently published.We kick off 2018 with some new publications. Here we feature a study where researchers use our kit to deliver G-protein-coupled receptor C (MrgprC) siRNA in vivo. This receptor is part of the TRPV1 pathway.Ying-jun Liu, Xiao-xi Lin, Jian-qiao Fang, and Fang Fang. Involvement of MrgprC in electroacupuncture analgesia for attenuating CFA-induced thermal hyperalgesia by suppressing the TRPV1 pathway. Pre-pub-full text PDFImage: Expression of TRPV1 in rats treated with MrgrprC s...
Source: siRNA and DsiRNA Transfection Efficiency - January 13, 2018 Category: Neuroscience Tags: inflammatory pain intrathecal delivery of siRNA Mas-related G-protein-coupled receptor C MrgrpfC TRPV1 Source Type: news

i-Fect Delivers siRNA to Study Angiogenesis in Brain Injury
This study revealed that LDHA, which is a known damage marker, promotes CNS-specific angiogenesis. LDHA-mediated angiogenesis depends on vimentin on the surface of vascular endothelial cells. The work described here proposes a novel mechanism by which neurodegeneration drives angiogenesis in the CNS.A mixture of ouri-FectTM and LDHA siRNA, in this study, were directly injected into mice cortexes: Hsiaoyun Lin, Rieko Muramatsu, Noriko Maedera, Hiroto Tsunematsu, Machika Hamaguchi, Yoshihisa Koyama, Mariko Kuroda, Kenji Ono, Makoto Sawada, Toshihide Yamashita.Extracellular Lactate Dehydrogenase A Release ...
Source: siRNA and DsiRNA Transfection Efficiency - February 19, 2018 Category: Neuroscience Tags: Angiogenesis Delivering siRNA to the CNS i-Fect Lactate Dehydrogenase A LDHA siRNA delivery to mouse brain Source Type: news

i-Fect™ Delivers Your siRNA Payload
Delivering siRNA to Dorsal Root Ganglia to Silence KV Receptors.Our i-Fect transfection kits continue to be used to optimize delivery in vivo and into hard to transfect cells like primary neurons. In these 2 latest expamples, researchers use i-Fect to deliver siRNA to KV Receptors in Rat DRGs. Knocking down these receptors enable the study of their role in pain modulation: John H. Winston, Sushil K. Sarna. Developmental Origins of Functional Dyspepsia-Like Gastric Hypersensitivity in Rats. Gastroenterology. Volume 144, Issue 3, March 2013, Pages 570–579.e3. dx.doi.org/10.1053/j.gastro.2012.11.001....intrathecal treatment...
Source: Neuromics - March 16, 2013 Category: Neuroscience Tags: siRNA delivery in-vivo Delivering siRNA to the CNS KV receptors. i-Fect Kv9.1 Potassium Channel Source Type: news

Using i-Fect to Knock Down GLT-1 Gene in vivo
Pain Researchers continue to use our i-FectTM Transfection Kit for Gene Expression AnalysisValproate Prevents Dysregulation of Spinal Glutamate and Reduces the Development of Hypersensitivity in Rats After Peripheral Nerve Injury. The Journal of Pain, Volume 14, Issue 11, November 2013, Pages 1485–1491 http://dx.doi.org/10.1016/j.jpain.2013.07.007.Researchers use the kit to deliver Glutamate Receptor 1 siRNA in vivo.The present study examined whether the histone deacetylase inhibitor valproate prevents downregulation of glutamate transporters in the primary cultured astrocytes and in the spinal cord after L5-L6 spinal ne...
Source: siRNA and DsiRNA Transfection Efficiency - November 6, 2013 Category: Neuroscience Tags: Delivering siRNA to the CNS GLT-1 Gene Glutamate Receptor 1 siRNA in vivo i-Fect intrathecal delivery of siRNA Source Type: news

i-Fect, BDNF and Irritable Bowel Syndrome (IBS)
Conclusion: Chronic prenatal stress followed by a second exposure to HeICS in adult offspring exacerbated visceral hypersensitivity (VHS) greater in female offspring that persisted longer than in male offspring. Chronic prenatal stress up-regulated BDNF expression in the lumbar-sacral dorsal horn that correlated with the exacerbation of VHS in female, but not in male offspring by increasing RNA Pol II binding and histone H3 acetylation, and decreasing histone deacetylase 1 association with the core promoter of BDNF in female offspring.
Source: siRNA and DsiRNA Transfection Efficiency - March 11, 2014 Category: Neuroscience Tags: BDNF;chronic stress;functional bowel disorders;iritable bowel syndrome;visceral hypersnsitivity i-Fect intrathecal delivery of siRNA Source Type: news

Preclinical development of siRNA therapeutics: Towards the match between fundamental science and engineered systems
Abstract: The evolution of synthetic RNAi faces the paradox of interfering with the human biological environment. Due to the fact that all cell physiological processes can be target candidates, silencing a precise biological pathway could be challenging if target selectivity is not properly addressed. Molecular biology has provided scientific tools to suppress some of the most critical issues in gene therapy, while setting the standards for siRNA clinical application. However, the protein down-regulation through the mRNA silencing is intimately related to the sequence-specific siRNA ability to interact accurately with the ...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - December 11, 2013 Category: Nanotechnology Authors: M. Videira, A. Arranja, D. Rafael, R. Gaspar Tags: Cell Biology and Genetics, Amino Acids, Nucleic Acids, siRNA Therapy, Basic Science vs. Engineering Source Type: research

Gene Expression Analysis For Neuroscientists
In Vitro and In Vivo StudiesNeuromics has a strong line up of Transfection Tools designed specifically for Neuroscientists. Neurons, Glia and Astrocytes are notoriously hard to transfect. We are proud of our track record.This is an excellent study for Neuroscience Researchers interested in using best methods: http://emboj.embopress.org/content/embojnl/31/15/3239/DC1/embed/inline-supplementary-material-1.pdf?download=true.  Here researchers delivered 14-3-3 siRNA sub units + i-Fect intrathecally. Here's specific knockdown results:Figure: A. Dose-dependent inhibition of 14-3-3-zeta (z) expression with ant...
Source: siRNA and DsiRNA Transfection Efficiency - June 25, 2014 Category: Neuroscience Tags: 14-3-3 zeta i-Fect in vivo RNAi intrathecal delivery of siRNA intravenous delivery of siRNA siRNA in vivo Transfection Source Type: news

Hybrid lipid–polymer nanoparticles for sustained siRNA delivery and gene silencing
Abstract: The development of controlled-release nanoparticle (NP) technologies has great potential to further improve the therapeutic efficacy of RNA interference (RNAi), by prolonging the release of small interfering RNA (siRNA) for sustained, long-term gene silencing. Herein, we present an NP platform with sustained siRNA-release properties, which can be self-assembled using biodegradable and biocompatible polymers and lipids. The hybrid lipid–polymer NPs showed excellent silencing efficacy, and the temporal release of siRNA from the NPs continued for over one month. When tested on luciferase-expressed HeLa cells and A...
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - March 19, 2014 Category: Nanotechnology Authors: Jinjun Shi, Yingjie Xu, Xiaoyang Xu, Xi Zhu, Eric Pridgen, Jun Wu, Alexander R. Votruba, Archana Swami, Bruce R. Zetter, Omid C. Farokhzad Tags: Pharmacology, Nano-Enabled Drug Delivery, Controlled Release, Polymer-siRNA Hybrid Source Type: research

Neuromics' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors for Gene Expression Analysis.I have shared the many genes researchers have studied using our Transfection Kits. These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More!We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of Neuropath...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news

EPO Protects Against Cerebral Ischemia
In this study data suggest the protective effects of EPO on NUV injuries are highly associated with the increase of p-Cx43, which improves GJIC to reduce neurotoxic substances: Ziyi Zhoua, Xiaobai Weib, Jun Xiang, Junpeng Gao, Lixin Wang, Jinsong You, Yefeng Cai , Dingfang Caid. Protection of erythropoietin against ischemic neurovascular unit injuries through the effects of connexin43. Biochemical and Biophysical Research Communications. doi:10.1016/j.bbrc.2015.02...The strands were incubated at 90°C for 5 min and then at 37°C for 1 h. SiRNA was prepared immediately before administration by mixing the RNA solution (1 μg...
Source: siRNA and DsiRNA Transfection Efficiency - February 18, 2015 Category: Neuroscience Tags: cerebral ischemia Connexin 43 Delivering siRNA to the CNS i-Fect iFect siRNA transfection of neurons Source Type: news

i-Fect Delivers siRNA in vitro and in vivo
In this study data suggest the protective effects of EPO on NUV injuries are highly associated with the increase of p-Cx43, which improves GJIC to reduce neurotoxic substances: Ziyi Zhoua, Xiaobai Weib, Jun Xiang, Junpeng Gao, Lixin Wang, Jinsong You, Yefeng Cai , Dingfang Caid. Protection of erythropoietin against ischemic neurovascular unit injuries through the effects of connexin43. Biochemical and Biophysical Research Communications. doi:10.1016/j.bbrc.2015.02...The strands were incubated at 90°C for 5 min and then at 37°C for 1 h. SiRNA was prepared immediately before administration by mixing the RNA solution (1 μg...
Source: Neuromics - February 21, 2015 Category: Neuroscience Tags: Cerebral Ischemia Connexin 43 Delivering siRNA to the CNS EPO HDAC2 i-Fect Stroke Source Type: news

Silencing Cytokines in-vivo with i-Fect
Knocking Down Cytokine to Study Pain ResponseWe have many unique applications published from researchers using our Transfection Kits in vitro and in vivo. Here researchers simultaneously 3 immune/inflammatory response cytokines in vivo: Byung Moon Choi, Soo Han Lee, Sang Mee An, Do Yang Park, Gwan Woo Lee, and Gyu-Jeong Noh. The time-course and RNA interference of TNF-α, IL-6, and IL-1β expression on neuropathic pain induced by L5 spinal nerve transection in rats. Korean J Anesthesiol. 2015 Apr;68(2):159-169. English. Published online March 30, 2015. http://dx.doi.org/10.4097/kjae.2015.68.2.159.Protocol: RNAs w...
Source: siRNA and DsiRNA Transfection Efficiency - April 7, 2015 Category: Neuroscience Tags: allodynia analgesia Gene Expression Analysis Gene Silencing i-Fect IL-1B IL-6 inflammatory pain intrathecal delivery of siRNA TNF-alpha Transfection Kits Source Type: news

Epigenetics and Pain Research
i-Fect Used to Study ImpactsOur i-Fect siRNA, miRNA and shRNA Trasfection Kit was recently used to study the impact of G9a-specific siRNA (AGUAACGGGCAUCAAUGC) on Mu Opioid Receptors: Yuhao Zhang, Shao-Rui Chen, Geoffroy Laumet, Hong Chen and Hui-Lin Pan. Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-Mediated Transcriptional Repression of µ-Opioid Receptors in Primary Sensory Neurons. First Published on February 25, 2016, doi: 10.1074/jbc.M115.711812... In some SNL rats, G9a-specific siRNA (4 µg) or the negative control siRNA was administered intrathecally. G9a-specific siRNA(AGUAACGGGCAUC...
Source: siRNA and DsiRNA Transfection Efficiency - March 24, 2016 Category: Neuroscience Tags: epigenetics Ga9 Mu Opioid Receptor inhibitor i-Fect iFect intrathecal delivery of siRNA Morphine neuropathic pain Source Type: news

Neuromics ' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors  for Gene Expression Analysis . I have shared the many genes researchers have studied using our Transfection Kits . These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More! We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of N...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news

siRNA Design Principles and Off-Target Effects
Short interfering RNAs (siRNAs) are a major research tool that allows for knock-down of target genes via selective mRNA destruction in almost all eukaryotic organisms. siRNAs typically consist of a synthetic ∼21 nucleotide (nt) RNA-duplex where one strand is designed with perfect complementarity to the target mRNA. Although siRNAs were initially thought to be very target-specific because of their design, it turned out during the last years that all siRNAs have a more or less pronounced intrinsic off-target activity which can make the interpretation of data from siRNA experiments difficult. Here we describe essential ru...
Source: Springer protocols feed by Pharmacology/Toxicology - February 26, 2013 Category: Drugs & Pharmacology Source Type: news

Western Blot Evaluation of siRNA Delivery by pH-Responsive Peptides
Gene silencing, via RNA interference (RNAi) technologies using small interfering RNA (siRNA), has been developed as an important tool for target identification and validation in drug discovery and has huge therapeutic potential. However, effective delivery into cells presents a major challenge to the use of siRNA. pH-responsive cell-penetrating peptides have attracted considerable attention in recent years as delivery vectors due to their ability to transport their cargos across the biological membrane and/or to promote endosomal escape and prevent lysosomal degradation. To evaluate the in vitro transfection efficiency of ...
Source: Springer protocols feed by Pharmacology/Toxicology - February 26, 2013 Category: Drugs & Pharmacology Source Type: news

BDNF and Recovery of Motor Function After Brian Injury
Researchers use i-FectTM to Study BDNF Silencing in MiceBrain injury that results in an initial behavioural deficit is frequently followed by spontaneous recovery. The intrinsic mechanism of this functional recovery has never been fully understood. Here, we show that reorganization of the corticospinal tract induced by target-derived brain-derived neurotrophic factor is crucial for spontaneous recovery of motor function following brain injury:  Masaki Ueno, Yasufumi Hayano1, Hiroshi Nakagawa and Toshihide Yamashita. Intraspinal rewiring of the corticospinal tract requires target-derived brain-derived neurotrophic...
Source: siRNA and DsiRNA Transfection Efficiency - February 27, 2013 Category: Neuroscience Tags: siRNA Delivery in vitro BDNF i-Fect Gene Silencing Brain Derived Neurotrophic Factor Gene in vivo siRNA Source Type: news

Therapeutic Potential Of SiRNA Drugs Boosted By Nanoparticles
New classes of drugs that can silence specific genes, such as small interfering RNAs (siRNAs), offer great therapeutic potential. But the specific delivery of siRNAs to target cells to exert their effects remains a significant challenge. A novel nanoparticle-based approach that enables more efficient delivery of siRNA drugs is presented in Nucleic Acid Therapeutics, a peer-reviewed journal from Mary Ann Liebert, Inc. publishers The article is available on the Nucleic Acid Therapeutics website...
Source: Health News from Medical News Today - April 15, 2013 Category: Consumer Health News Tags: Genetics Source Type: news

Alnylam buys Merck's Sirna Therapeutics
Alnylam Pharmaceuticals has acquired Merck's subsidiary Sirna Therapeutics, comprising intellectual property and RNAi assets, including pre-clinical therapeutic candidates, chemistry, siRNA-conjugate and other delivery technologies.
Source: Pharmaceutical Technology - January 13, 2014 Category: Pharmaceuticals Source Type: news

Merck Dumps Sirna RNAi Assets for $175M
Merck has agreed to sell its Sirna Therapeutics assets to Alnylam Pharmaceuticals, a leading RNAi therapeutics company, for an upfront payment of $175 million in cash and equity.
Source: PharmaManufacturing.com - January 14, 2014 Category: Pharmaceuticals Source Type: news

Direct Imaging of siRNA Electrotransfer at the Single-Cell Level
In this study, we investigated, by direct visualization at the single-cell level, the electro-delivery of Alexa Fluor 546-labeled siRNA into murine melanoma cells stably expressing the enhanced green fluorescent protein (EGFP) as a target gene. The electrotransfer of siRNA was quantified by time-lapse fluorescence microscopy and was correlated with the silencing of EGFP expression. A direct transfer into the cell cytoplasm of the negatively charged siRNA was observed across the plasma membrane exclusively on the side facing the cathode. The oligonucleotide then freely diffused across the cytosol. Therefore, we show that th...
Source: Springer protocols feed by Genetics/Genomics - February 13, 2014 Category: Genetics & Stem Cells Source Type: news

Electroporation-Mediated siRNA Delivery into Tumors
Electroporation-mediated gene transfer (electro-transfection) is a powerful tool to introduce nucleic acid compounds such as plasmid DNAs, antisense oligonucleotides, and short interfering RNAs (siRNAs) into the cells. Electro-transfection is a physical gene transfer method that utilizes an electrostatic field generated with an electroporator apparatus. Here, we demonstrate a practical protocol for electro-transfection (electro-delivery) of siRNA into cells in vivo and further demonstrate the application of the method to cancer therapy. We successfully developed an original electrode (the plate and fork-type electrode) and...
Source: Springer protocols feed by Genetics/Genomics - February 13, 2014 Category: Genetics & Stem Cells Source Type: news

Intranasal Delivery of Chitosan–siRNA Nanoparticle Formulation to the Brain
Neurodegeneration is characterized by a progressive loss of neuron structure and function. Most neurodegenerative diseases progress slowly over the time. There is currently no cure available for any neurodegenerative disease, and the existing therapeutic interventions only alleviate the symptoms of the disease. The advances in the drug discovery research have come to a halt with a lack of effective means to deliver drugs at the targeted site. In addition, the route of delivering the drugs is equally important as most invasive techniques lead to postoperative complications. This chapter focuses on a non-invasive, intranasal...
Source: Springer protocols feed by Biotechnology - February 25, 2014 Category: Biotechnology Source Type: news

Microfluidic-Based Manufacture of siRNA-Lipid Nanoparticles for Therapeutic Applications
A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles.
Source: Springer protocols feed by Biotechnology - February 25, 2014 Category: Biotechnology Source Type: news

Amphipathic Homopolymers for siRNA Delivery: Probing Impact of Bifunctional Polymer Composition on Transfection.
In this study, we systematically explore the influence of the lipophilic group on the siRNA transfection properties of the polycationic-based delivery vectors. For this, a novel and modular synthetic strategy was developed for the preparation of polymers carrying a cationic site and a lipophilic group at each polymer repeat unit. These bifunctional polymers could form a complex with siRNA and deliver it to human colon carcinoma cells (HT-29-luc). In general, transfection capability increased with an increase in the chain length of the lipophilic moiety. The best transfection agent, a polymer containing ammonium groups and ...
Source: Biomacromolecules - April 22, 2014 Category: Biochemistry Authors: Buerkli C, Lee SH, Moroz E, Stuparu MC, Leroux JC, Khan A Tags: Biomacromolecules Source Type: research

Tumor-penetrating codelivery of siRNA and paclitaxel with ultrasound-responsive nanobubbles hetero-assembled from polymeric micelles and liposomes.
Abstract Drug resistance is a big problem in systemic chemotherapy of hepatocellular carcinoma (HCC), and nanomedicines loaded with both chemotherapeutic agents (e.g. paclitaxel, PTX) and siRNA's targeting antiapoptosis genes (e.g. BCL-2) possess the advantages to simultaneously overcome the efflux pump-mediated drug resistance and antiapoptosis-related drug resistance. However, tumor-penetrating drug delivery with this type of nanomedicines is extremely difficult due to their relatively big size compared to the single drug-loaded nanomedicines. Aiming at address this problem, US-responsive nanobubbles encapsulati...
Source: Biomaterials - April 17, 2014 Category: Materials Science Authors: Yin T, Wang P, Li J, Wang Y, Zheng B, Zheng R, Cheng D, Shuai X Tags: Biomaterials Source Type: research

Interactions between the non-seed region of siRNA and RNA-binding RLC/RISC proteins, Ago and TRBP, in mammalian cells
Small interfering RNA (siRNA)-based RNA interference (RNAi) is widely used for target gene silencing in various organisms. We previously showed that 8-nt-long 5' proximal nucleotides, which include seed sequence (positions 2–8 from the 5' end of guide strand), and the complementary sequence of the passenger strand are capable of being simultaneously replaced with cognate deoxyribonucleotides without any substantial loss of gene silencing. In the present study, examination was made of RNA requirements in the non-seed region of siRNA. The non-seed region of siRNA was found to be subdivided into four domains, in which t...
Source: Nucleic Acids Research - April 30, 2014 Category: Research Authors: Takahashi, T., Zenno, S., Ishibashi, O., Takizawa, T., Saigo, K., Ui-Tei, K. Tags: RNA Source Type: research

Effects of DCLK1-siRNA{+/-}Curcumin on Cancer Stem Cells
Curcumin is known to induce apoptosis of cancer cells by different mechanisms, but its effects on cancer stem cells (CSC) have been less investigated. Here, we report that curcumin promotes the survival of DCLK1-positive colon CSCs, potentially confounding application of its anticancer properties. At optimal concentrations, curcumin greatly reduced expression levels of stem cell markers (DCLK1/CD44/ALDHA1/Lgr5/Nanog) in three-dimensional spheroid cultures and tumor xenografts derived from colon cancer cells. However, curcumin unexpectedly induced proliferation and autophagic survival of a subset of DCLK1-positive CSCs. Sph...
Source: Cancer Research - April 30, 2014 Category: Cancer & Oncology Authors: Kantara, C., O'Connell, M., Sarkar, S., Moya, S., Ullrich, R., Singh, P. Tags: Prevention and Epidemiology Source Type: research

Low-density lipoprotein-coupled N-succinyl chitosan nanoparticles co-delivering siRNA and doxorubicin for hepatocyte-targeted therapy.
In this study, low-density lipoprotein (LDL) was isolated from human plasma and loaded with cholesterol-conjugated siRNA to silence the multidrug resistant gene of tumors. Chol-siRNA/LDL-coupled N-succinyl chitosan nanoparticles loaded with doxorubicin (Dox-siRNA/LDL-SCS-NPs) were then prepared and characterised. The Dox-siRNA/LDL-SCS-NPs had average particle size of 206.4 ± 9.2 nm, entrapment efficiency of 71.06% ± 1.42%, and drug-loading amount of 12.35% ± 0.87%. In vitro antitumor activity revealed that cell growth was significantly inhibited. The accumulation of Dox by fluorescence microscopy and flow cytome...
Source: Biomaterials - April 24, 2014 Category: Materials Science Authors: Zhu QL, Zhou Y, Guan M, Zhou XF, Yang SD, Liu Y, Chen WL, Zhang CG, Yuan ZQ, Liu C, Zhu AJ, Zhang XN Tags: Biomaterials Source Type: research

Amelioration of Colorectal Cancer Using Negative Lipidoid Nanoparticles to Encapsulate siRNA Against APRIL by Enema Delivery Mode.
This study reveals APRIL to be a potential anti-CRC target by in vivo experiments, and suggests that the application of similar modes of siRNA delivery may be feasible in other therapeutic settings. PMID: 24771268 [PubMed - as supplied by publisher]
Source: Pathology Oncology Research - April 26, 2014 Category: Pathology Authors: Ding W, Wang G, Shao K, Wang F, Huang H, Ju S, Cong H, Wang H Tags: Pathol Oncol Res Source Type: research

Effect of the guide strand 3{'}-end structure on the gene silencing potency of asymmetric siRNA
In this study, we explored the effect of the guide strand 3’-end structure on the gene silencing potency of asiRNA. Interestingly, asiRNAs with a 21 nucleotide (nt) guide strand solely composed of RNA resulted in gene silencing more than 6-fold more efficient compared with the corresponding asiRNA guide strand harboring a deoxythymidine dinucleotide (dTdT) at its 3'-end. We demonstrated that the molecular basis of potency of the asiRNA with a 21 nt guide strand composed solely of RNA was due to the enhanced formation of the RNA- induced silencing complex (RISC) and increased affinity towards human Argonaute2 (hAgo2)...
Source: BJ Gene - May 6, 2014 Category: Biochemistry Authors: S Hong, J Park, S Yun, C Lee, C Shin, D Lee Tags: BJ Biomolecules Source Type: research

SiRNA-phospholipid conjugates for gene and drug delivery in cancer treatment.
In this study, the thiol-modified sense and antisense siRNA are chemically conjugated with phospholipids to form sense and antisense siRNA-phospholipid, and then these sense or antisense siRNA-phospholipids with equal amounts are annealed to generate siRNA-phospholipids. The siRNA-phospholipids can serve dual functions as agents that can silence gene expression and as a component of nanoparticles to embed hydrophobic anticancer drugs to cure tumor. siRNA-phospholipids together with cationic lipids and DSPE-PEG2000 fuse around PLGA to form siRNA-phospholipids enveloped nanoparticles (siRNA-PCNPs), which can deliver siRNAs a...
Source: Biomaterials - May 2, 2014 Category: Materials Science Authors: Liu H, Li Y, Mozhi A, Zhang L, Liu Y, Xu X, Xing J, Liang X, Ma G, Yang J, Zhang X Tags: Biomaterials Source Type: research

Biocompatible, Chimeric Peptide-Condensed Supramolecular Nanoparticles for Tumor Cell-Specific siRNA Delivery and Gene Silencing
Chem. Commun., 2014, Accepted ManuscriptDOI: 10.1039/C4CC01061B, CommunicationHangxiang Wang, Wei Chen, Haiyang Xie, Xuyong Wei, Shengyong Yin, Lin Zhou, Xiao Xu, Shusen ZhengWith the increased potential of RNA interference (RNAi) as a therapeutic modality, there is broad interest in the development of biocompatible vehicles for efficient, cell-specific delivery of siRNA agents. To...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - May 12, 2014 Category: Chemistry Authors: Hangxiang Wang Source Type: research

Connexin43 siRNA promotes HUVEC proliferation and inhibits apoptosis induced by ox-LDL: an involvement of ERK signaling pathway.
In this study, we investigated the effects of ox-LDL on connexin43 and the mechanisms of connexin43 siRNA-inhibited apoptosis induced by ox-LDL in human umbilical vein endothelial cell (HUVEC), to clarify the role of connexin43 in atherosclerosis. Our results showed that ox-LDL significantly inhibited the growth and promoted apoptosis of HUVEC in a dose-dependent manner. Also, ox-LDL upregulated the expression of connexin43. Furthermore, knockdown connexin43 by siRNA promoted proliferation and inhibited apoptosis in ox-LDL-stimulated HUVEC. Moreover, the level of phosphor-ERK1/2 and connexin43 was remarkably attenuated by ...
Source: Molecular and Cellular Biochemistry - May 16, 2014 Category: Biochemistry Authors: Yin G, Yang X, Li B, Yang M, Ren M Tags: Mol Cell Biochem Source Type: research

Targeted electro-delivery of oligonucleotides for RNA Interference: siRNA and antimiR.
Abstract For more than a decade, the understanding of RNA interference (RNAi) has been a growing field of interest. Micro-RNAs (miRNAs) are small regulatory RNAs that play an important role in disease development and progression and therefore represent a potential new class of therapeutic targets. However, delivery of RNAi-based oligonucleotides is one of the most challenging hurdles to RNAi-based drug development. Electropermeabilization (EP) is recognized as a successful non-viral method to transfer nucleic acids into living cells both in vitro and in vivo. EP is the direct application of electric pulses to cell...
Source: Advanced Drug Delivery Reviews - May 9, 2014 Category: Drugs & Pharmacology Authors: Chabot S, Teissié J, Golzio M Tags: Adv Drug Deliv Rev Source Type: research

Multivalent comb-type aptamer-siRNA conjugates for efficient and selective intracellular delivery.
In this study, a simple and efficient strategy for selective intracellular delivery of RNA therapeutics into target cancer cells was designed using direct complementary base pairing between chemically conjugated multimeric antisense strands and aptamer-incorporating sense strands. PMID: 24830507 [PubMed - as supplied by publisher]
Source: Chemical Communications - May 16, 2014 Category: Chemistry Authors: Yoo H, Jung H, Kim SA, Mok H Tags: Chem Commun (Camb) Source Type: research

Effect of siRNA PERK on Fluoride-Induced Osteoblastic Differentiation in OS732 Cells.
This study proved that the mechanism underlying fluoride induced osteoblastic and osteoclastic differentiation possible was due to activation of ALP and RANKL mediated by PERK in OS732 cells. PMID: 24838929 [PubMed - as supplied by publisher]
Source: Biological Trace Element Research - May 18, 2014 Category: Biology Authors: Lü P, Li X, Ruan L, Xu H, Liu Q Tags: Biol Trace Elem Res Source Type: research

Effective knock down of matrix metalloproteinase‐13 by an intra‐articular injection of small interfering RNA (siRNA) in a murine surgically‐induced osteoarthritis model
This study investigated the effect of MMP‐13 gene knock down on cartilage degradation by injecting small interfering RNA (siRNA) into knee joints in a mouse model of osteoarthritis (OA). OA was induced in male C57BL/6 mice by destabilization of medial meniscus (DMM) surgery. Change of Mmp13 expression over time was determined by qPCR analysis from 3 days to 6 weeks after surgery. Mmp13 and control chemically modified siRNA were injected into the knee joint 1 week after surgery and expression levels were assessed in synovium by qPCR 48 h later. Cartilage degradation was histologically assessed 8 weeks after DMM surgery ...
Source: Journal of Orthopaedic Research - May 23, 2014 Category: Orthopaedics Authors: Ryuichiro Akagi, Takahisa Sasho, Masahiko Saito, Jun Endo, Satoshi Yamaguchi, Yuta Muramatsu, Shunsuke Mukoyama, Yorikazu Akatsu, Joe Katsuragi, Taisuke Fukawa, Kazuhisa Takahashi Tags: Research Article Source Type: research

Antibody-modified lipid nanoparticles for selective delivery of siRNA to tumors expressing membrane-anchored form of HB-EGF.
Abstract An Fab' antibody against heparin-binding epidermal growth factor-like growth factor (HB-EGF) was applied to achieve advanced tumor-targeted delivery of siRNA. Lipid nanoparticles (LNP) encapsulating siRNA (LNP-siRNA) were prepared, pegylated, and surface modified with Fab' fragments of anti-HB-EGF antibody (αHB-EGF LNP-siRNA). αHB-EGF LNP-siRNA showed high-binding affinity to recombinant human HB-EGF in a Biacore assay. In addition, αHB-EGF LNP-siRNA selectively associated with cells expressing HB-EGF in vitro. Confocal microscopic images showed that siRNA formulated in αHB-EGF LNP-siRNA was efficient...
Source: Biochemical and Biophysical Research communications - May 19, 2014 Category: Biochemistry Authors: Okamoto A, Asai T, Kato H, Ando H, Minamino T, Mekada E, Oku N Tags: Biochem Biophys Res Commun Source Type: research

Tumor-specific delivery of siRNA using supramolecular assembly of hyaluronic acid nanoparticles and 2b RNA-binding protein/siRNA complexes.
Abstract Anticancer therapeutics delivering exogenous siRNA have been explored to suppress the tumor-associated genes, but several limitations of siRNA delivery such as tumor-targeted delivery, controlled siRNA release at the sites of interest, or instabilities of siRNA in physiological fluids should be preferentially addressed for its clinical applications. As an attempt to meet these criteria, we designed a supramolecular assembly, which was composed of cholesterol-bearing hyaluronic acid (HA-Chol) conjugates and 2b RNA-binding protein (2b)/siRNA complexes. In contrast to the traditional siRNA polyplexes using e...
Source: Biomaterials - May 19, 2014 Category: Materials Science Authors: Choi KM, Jang M, Kim JH, Ahn HJ Tags: Biomaterials Source Type: research

SiRNA-Mediated Flotillin-2 (Flot2) Downregulation Inhibits Cell Proliferation, Migration, and Invasion in Gastric Carcinoma Cells.
In conclusion, the present study suggests that the Flot2 protein expression is significantly correlated with cancer progression and poor prognosis in gastric carcinomas, probably due to its role in the regulation of cell proliferation, migration, and invasion in gastric carcinoma cells. PMID: 24854103 [PubMed - in process]
Source: Oncology Research - May 28, 2014 Category: Cancer & Oncology Authors: Cao K, Xie D, Cao P, Zou Q, Lu C, Xiao S, Zhou J, Peng X Tags: Oncol Res Source Type: research

One siRNA pool targeting the {lambda} constant region stops {lambda} light-chain production and causes terminal endoplasmic reticulum stress
In systemic light-chain amyloidosis, light chains produced by clonal plasma cells cause organ damage and early death. In pursuit of novel therapy, we developed 1 pool of short interfering RNA (siRNA) targeting the constant region of light chains that substantially and promptly reduces -light-chain production and secretion by human plasma cells regardless of sequence diversity. In clones producing intact immunoglobulin G (IgG) antibodies (containing paired heavy and light chains), the secretion of intact antibodies is reduced, and all 3 branches of the unfolded protein response are activated by accumulation of unpaired IgG ...
Source: Blood - May 29, 2014 Category: Hematology Authors: Zhou, P., Ma, X., Iyer, L., Chaulagain, C., Comenzo, R. L. Tags: Multiple Myeloma, Lymphoid Neoplasia Source Type: research

Downregulation of survivin by siRNA inhibits invasion and promotes apoptosis in neuroblastoma SH-SY5Y cells.
Abstract Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and invasion. Previous studies found that survivin is highly expressed in some malignant neuroblastomas and is correlated with poor prognosis. The aim of this study was to investigate whether survivin could serve as a potential therapeutic target of human neur...
Source: Braz J Med Biol Res - May 23, 2014 Category: Research Authors: Zhang L, Liang H, Cao W, Xu R, Ju XL Tags: Braz J Med Biol Res Source Type: research

Direct cytosolic siRNA delivery by reconstituted high density lipoprotein for target-specific therapy of tumor angiogenesis.
We described here the mechanisms by which small interfering RNA (siRNA) molecules incorporated in reconstituted high density lipoprotein (rHDL) were efficiently transferred into the cytoplasm of cells to perform target-specific therapy of tumor angiogenesis. Using fluorescent-tagged apolipoprotein A-I (apoA-I) and cholesterol-conjugated siRNA (Chol-siRNA), it was confirmed with FACS and confocal microscopic measurements that Chol-siRNA-loaded rHDL nanoparticles (rHDL/Chol-siRNA complexes) were successfully established and apoA-I certainly was attached to the surface of Chol-siRNA-loaded lipoplexes (Lipos/Chol-siRNA complex...
Source: Biomaterials - May 27, 2014 Category: Materials Science Authors: Ding Y, Wang Y, Zhou J, Gu X, Wang W, Liu C, Bao X, Wang C, Li Y, Zhang Q Tags: Biomaterials Source Type: research