ITPRIP promotes glioma progression by linking MYL9 to DAPK1 inhibition
In this study, we identified the existence of DAPK1-inositol 1,4,5-trisphosphate receptor (IP3R)-interacting protein (ITPRIP) -myosin regulatory light polypeptide 9 (MYL9) complex in malignant glioma cells. Lentivirus co-infection and coimmunoprecipitation showed that ITPRIP bound with the death domain (DD) of DAPK1 in vitro. Further, dissociating ITPRIP-DAPK1 interaction inhibited glioma tumor growth in vitro but not in vivo. Moreover, knockdown of ITPRIP or DAPK1 impaired the ternary complex formation, whereas MYL9 knockdown did not affect ITPRIP-DAPK1 association. We further found that ITPRIP recruited MYL9 to the kinas...
Source: Cellular Signalling - June 10, 2021 Category: Cytology Authors: Changchun Cao Kang He Shaoxun Li Qianqian Ge Lei Liu Zhengwei Zhang Hui Zhang Xinwen Wang Xiaoyang Sun Lianshu Ding Source Type: research

ITPRIP promotes glioma progression by linking MYL9 to DAPK1 inhibition
In this study, we identified the existence of DAPK1-inositol 1,4,5-trisphosphate receptor (IP3R)-interacting protein (ITPRIP) -myosin regulatory light polypeptide 9 (MYL9) complex in malignant glioma cells. Lentivirus co-infection and coimmunoprecipitation showed that ITPRIP bound with the death domain (DD) of DAPK1 in vitro. Further, dissociating ITPRIP-DAPK1 interaction inhibited glioma tumor growth in vitro but not in vivo. Moreover, knockdown of ITPRIP or DAPK1 impaired the ternary complex formation, whereas MYL9 knockdown did not affect ITPRIP-DAPK1 association. We further found that ITPRIP recruited MYL9 to the kinas...
Source: Cellular Signalling - June 10, 2021 Category: Cytology Authors: Changchun Cao Kang He Shaoxun Li Qianqian Ge Lei Liu Zhengwei Zhang Hui Zhang Xinwen Wang Xiaoyang Sun Lianshu Ding Source Type: research

ITPRIP promotes glioma progression by linking MYL9 to DAPK1 inhibition
In this study, we identified the existence of DAPK1-inositol 1,4,5-trisphosphate receptor (IP3R)-interacting protein (ITPRIP) -myosin regulatory light polypeptide 9 (MYL9) complex in malignant glioma cells. Lentivirus co-infection and coimmunoprecipitation showed that ITPRIP bound with the death domain (DD) of DAPK1 in vitro. Further, dissociating ITPRIP-DAPK1 interaction inhibited glioma tumor growth in vitro but not in vivo. Moreover, knockdown of ITPRIP or DAPK1 impaired the ternary complex formation, whereas MYL9 knockdown did not affect ITPRIP-DAPK1 association. We further found that ITPRIP recruited MYL9 to the kinas...
Source: Cellular Signalling - June 10, 2021 Category: Cytology Authors: Changchun Cao Kang He Shaoxun Li Qianqian Ge Lei Liu Zhengwei Zhang Hui Zhang Xinwen Wang Xiaoyang Sun Lianshu Ding Source Type: research

Metal dependent protein phosphatase PPM family in cardiac health and diseases
Cell Signal. 2021 Jun 6;85:110061. doi: 10.1016/j.cellsig.2021.110061. Online ahead of print.ABSTRACTProtein phosphorylation and dephosphorylation is central to signal transduction in nearly every aspect of cellular function, including cardiovascular regulation and diseases. While protein kinases are often regarded as the molecular drivers in cellular signaling with high specificity and tight regulation, dephosphorylation mediated by protein phosphatases is also gaining increasing appreciation as an important part of the signal transduction network essential for the robustness, specificity and homeostasis of cell signaling...
Source: Cellular Signalling - June 6, 2021 Category: Cytology Authors: Chen Gao Nancy Cao Yibin Wang Source Type: research

Metal dependent protein phosphatase PPM family in cardiac health and diseases
Cell Signal. 2021 Jun 3:110061. doi: 10.1016/j.cellsig.2021.110061. Online ahead of print.ABSTRACTProtein phosphorylation and dephosphorylation is central to signal transduction in nearly every aspect of cellular function, including cardiovascular regulation and diseases. While protein kinases are often regarded as the molecular drivers in cellular signaling with high specificity and tight regulation, dephosphorylation mediated by protein phosphatases is also gaining increasing appreciation as an important part of the signal transduction network essential for the robustness, specificity and homeostasis of cell signaling. M...
Source: Cellular Signalling - June 6, 2021 Category: Cytology Authors: Chen Gao Nancy Cao Yibin Wang Source Type: research

Metal dependent protein phosphatase PPM family in cardiac health and diseases
Cell Signal. 2021 Jun 3:110061. doi: 10.1016/j.cellsig.2021.110061. Online ahead of print.ABSTRACTProtein phosphorylation and dephosphorylation is central to signal transduction in nearly every aspect of cellular function, including cardiovascular regulation and diseases. While protein kinases are often regarded as the molecular drivers in cellular signaling with high specificity and tight regulation, dephosphorylation mediated by protein phosphatases is also gaining increasing appreciation as an important part of the signal transduction network essential for the robustness, specificity and homeostasis of cell signaling. M...
Source: Cellular Signalling - June 6, 2021 Category: Cytology Authors: Chen Gao Nancy Cao Yibin Wang Source Type: research

Metal dependent protein phosphatase PPM family in cardiac health and diseases
Cell Signal. 2021 Jun 3:110061. doi: 10.1016/j.cellsig.2021.110061. Online ahead of print.ABSTRACTProtein phosphorylation and dephosphorylation is central to signal transduction in nearly every aspect of cellular function, including cardiovascular regulation and diseases. While protein kinases are often regarded as the molecular drivers in cellular signaling with high specificity and tight regulation, dephosphorylation mediated by protein phosphatases is also gaining increasing appreciation as an important part of the signal transduction network essential for the robustness, specificity and homeostasis of cell signaling. M...
Source: Cellular Signalling - June 6, 2021 Category: Cytology Authors: Chen Gao Nancy Cao Yibin Wang Source Type: research

YTHDF2 mediates LPS-induced osteoclastogenesis and inflammatory response via the NF- κB and MAPK signaling pathways
Cell Signal. 2021 Jun 2;85:110060. doi: 10.1016/j.cellsig.2021.110060. Online ahead of print.ABSTRACTAberrant elevation of osteoclast differentiation and function is responsible for disrupting bone homeostasis in various inflammatory bone diseases. YTH domain family 2 (YTHDF2) is a well-known m6A-binding protein that plays an essential role in regulating cell differentiation and inflammatory processes by mediating mRNA degradation. However, the regulatory role of YTHDF2 in inflammatory osteoclast differentiation remains unelucidated. Here, we detected the expression of m6A-related genes and found that YTHDF2 was upregulate...
Source: Cellular Signalling - June 5, 2021 Category: Cytology Authors: Caihong Fang Mingli He Di Li Qiong Xu Source Type: research

YTHDF2 mediates LPS-induced osteoclastogenesis and inflammatory response via the NF- κB and MAPK signaling pathways
Cell Signal. 2021 Jun 2:110060. doi: 10.1016/j.cellsig.2021.110060. Online ahead of print.ABSTRACTAberrant elevation of osteoclast differentiation and function is responsible for disrupting bone homeostasis in various inflammatory bone diseases. YTH domain family 2 (YTHDF2) is a well-known m6A-binding protein that plays an essential role in regulating cell differentiation and inflammatory processes by mediating mRNA degradation. However, the regulatory role of YTHDF2 in inflammatory osteoclast differentiation remains unelucidated. Here, we detected the expression of m6A-related genes and found that YTHDF2 was upregulated i...
Source: Cellular Signalling - June 5, 2021 Category: Cytology Authors: Caihong Fang Mingli He Di Li Qiong Xu Source Type: research

Roles of individual human Dishevelled paralogs in the Wnt signalling pathways
Cell Signal. 2021 May 31:110058. doi: 10.1016/j.cellsig.2021.110058. Online ahead of print.ABSTRACTDishevelled (DVL) proteins are key mediators of most Wnt pathways. In all vertebrates, three DVL paralogs are present (DVL1, DVL2 and DVL3) but it is poorly defined to what extent they are functionally redundant. Here, we generated T-REx HEK 293 cells with only one DVL paralog (i.e., DVL1-only, DVL2-only, and DVL3-only) and compared their response to Wnt-3a and Wnt-5a ligands with wild type and DVL triple knockout cells. We show that DVL is essential, in addition to the previously shown Wnt-3a-induced phosphorylation of LRP6 ...
Source: Cellular Signalling - June 3, 2021 Category: Cytology Authors: Petra Pacl íková Tomasz Witold Radaszkiewicz David Pot ěšil Jakub Harno š Zbyn ěk Zdráhal V ítězslav Bryja Source Type: research

YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression
CONCLUSION: Runx3-miR-148a-3p axis targeted YBX1 to modulate NSCLC progression.PMID:34082012 | DOI:10.1016/j.cellsig.2021.110049 (Source: Cellular Signalling)
Source: Cellular Signalling - June 3, 2021 Category: Cytology Authors: Hong-Bo Su Guan-Zhi Fan Jin Huang Xue-Shan Qiu Source Type: research

Roles of individual human Dishevelled paralogs in the Wnt signalling pathways
Cell Signal. 2021 May 31:110058. doi: 10.1016/j.cellsig.2021.110058. Online ahead of print.ABSTRACTDishevelled (DVL) proteins are key mediators of most Wnt pathways. In all vertebrates, three DVL paralogs are present (DVL1, DVL2 and DVL3) but it is poorly defined to what extent they are functionally redundant. Here, we generated T-REx HEK 293 cells with only one DVL paralog (i.e., DVL1-only, DVL2-only, and DVL3-only) and compared their response to Wnt-3a and Wnt-5a ligands with wild type and DVL triple knockout cells. We show that DVL is essential, in addition to the previously shown Wnt-3a-induced phosphorylation of LRP6 ...
Source: Cellular Signalling - June 3, 2021 Category: Cytology Authors: Petra Pacl íková Tomasz Witold Radaszkiewicz David Pot ěšil Jakub Harno š Zbyn ěk Zdráhal V ítězslav Bryja Source Type: research

YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression
CONCLUSION: Runx3-miR-148a-3p axis targeted YBX1 to modulate NSCLC progression.PMID:34082012 | DOI:10.1016/j.cellsig.2021.110049 (Source: Cellular Signalling)
Source: Cellular Signalling - June 3, 2021 Category: Cytology Authors: Hong-Bo Su Guan-Zhi Fan Jin Huang Xue-Shan Qiu Source Type: research

Complex functionality of protein phosphatase 1 isoforms in the heart
Cell Signal. 2021 May 29:110059. doi: 10.1016/j.cellsig.2021.110059. Online ahead of print.ABSTRACTProtein phosphatase 1(PP1) is a key regulator of cardiac function through dephosphorylating serine/threonine residues within target proteins to oppose the function of protein kinases. Studies from failing hearts of animal models and human patients have demonstrated significant increase of PP1 activity, while elevated PP1 activity in transgenic mice leads to cardiac dysfunction, suggesting that PP1 might be a therapeutic target to ameliorate cardiac dysfunction in failing hearts. In fact, cardiac overexpression of inhibitor 1,...
Source: Cellular Signalling - June 1, 2021 Category: Cytology Authors: Ruijie Liu Source Type: research

Complex functionality of protein phosphatase 1 isoforms in the heart
Cell Signal. 2021 May 29:110059. doi: 10.1016/j.cellsig.2021.110059. Online ahead of print.ABSTRACTProtein phosphatase 1(PP1) is a key regulator of cardiac function through dephosphorylating serine/threonine residues within target proteins to oppose the function of protein kinases. Studies from failing hearts of animal models and human patients have demonstrated significant increase of PP1 activity, while elevated PP1 activity in transgenic mice leads to cardiac dysfunction, suggesting that PP1 might be a therapeutic target to ameliorate cardiac dysfunction in failing hearts. In fact, cardiac overexpression of inhibitor 1,...
Source: Cellular Signalling - June 1, 2021 Category: Cytology Authors: Ruijie Liu Source Type: research

Cigarette smoke extract-induced downregulation of p300 is responsible for the impaired inflammatory cytokine response of macrophages
Cell Signal. 2021 May 24:110050. doi: 10.1016/j.cellsig.2021.110050. Online ahead of print.ABSTRACTPatients with chronic obstructive pulmonary disease (COPD) are susceptible to infection owing to the impaired immune function of alveolar macrophages. This is presumed to be caused, at least partially, by cigarette smoke (CS), which is a major risk factor for COPD. Although CS has been reported to inhibit Toll-like receptor (TLR) function and phagocytosis in macrophages, the molecular mechanism of CS-mediated impairment of macrophage immune function has not been completely elucidated. We investigated the effects of CS extract...
Source: Cellular Signalling - May 27, 2021 Category: Cytology Authors: Kyoung-Hee Lee Jisu Woo Ji Yeon Kim Chang-Hoon Lee Chul-Gyu Yoo Source Type: research

Cigarette smoke extract-induced downregulation of p300 is responsible for the impaired inflammatory cytokine response of macrophages
Cell Signal. 2021 May 24:110050. doi: 10.1016/j.cellsig.2021.110050. Online ahead of print.ABSTRACTPatients with chronic obstructive pulmonary disease (COPD) are susceptible to infection owing to the impaired immune function of alveolar macrophages. This is presumed to be caused, at least partially, by cigarette smoke (CS), which is a major risk factor for COPD. Although CS has been reported to inhibit Toll-like receptor (TLR) function and phagocytosis in macrophages, the molecular mechanism of CS-mediated impairment of macrophage immune function has not been completely elucidated. We investigated the effects of CS extract...
Source: Cellular Signalling - May 27, 2021 Category: Cytology Authors: Kyoung-Hee Lee Jisu Woo Ji Yeon Kim Chang-Hoon Lee Chul-Gyu Yoo Source Type: research

Cigarette smoke extract-induced downregulation of p300 is responsible for the impaired inflammatory cytokine response of macrophages
Cell Signal. 2021 May 24:110050. doi: 10.1016/j.cellsig.2021.110050. Online ahead of print.ABSTRACTPatients with chronic obstructive pulmonary disease (COPD) are susceptible to infection owing to the impaired immune function of alveolar macrophages. This is presumed to be caused, at least partially, by cigarette smoke (CS), which is a major risk factor for COPD. Although CS has been reported to inhibit Toll-like receptor (TLR) function and phagocytosis in macrophages, the molecular mechanism of CS-mediated impairment of macrophage immune function has not been completely elucidated. We investigated the effects of CS extract...
Source: Cellular Signalling - May 27, 2021 Category: Cytology Authors: Kyoung-Hee Lee Jisu Woo Ji Yeon Kim Chang-Hoon Lee Chul-Gyu Yoo Source Type: research

Cigarette smoke extract-induced downregulation of p300 is responsible for the impaired inflammatory cytokine response of macrophages
Cell Signal. 2021 May 24:110050. doi: 10.1016/j.cellsig.2021.110050. Online ahead of print.ABSTRACTPatients with chronic obstructive pulmonary disease (COPD) are susceptible to infection owing to the impaired immune function of alveolar macrophages. This is presumed to be caused, at least partially, by cigarette smoke (CS), which is a major risk factor for COPD. Although CS has been reported to inhibit Toll-like receptor (TLR) function and phagocytosis in macrophages, the molecular mechanism of CS-mediated impairment of macrophage immune function has not been completely elucidated. We investigated the effects of CS extract...
Source: Cellular Signalling - May 27, 2021 Category: Cytology Authors: Kyoung-Hee Lee Jisu Woo Ji Yeon Kim Chang-Hoon Lee Chul-Gyu Yoo Source Type: research

Cigarette smoke extract-induced downregulation of p300 is responsible for the impaired inflammatory cytokine response of macrophages
Cell Signal. 2021 May 24:110050. doi: 10.1016/j.cellsig.2021.110050. Online ahead of print.ABSTRACTPatients with chronic obstructive pulmonary disease (COPD) are susceptible to infection owing to the impaired immune function of alveolar macrophages. This is presumed to be caused, at least partially, by cigarette smoke (CS), which is a major risk factor for COPD. Although CS has been reported to inhibit Toll-like receptor (TLR) function and phagocytosis in macrophages, the molecular mechanism of CS-mediated impairment of macrophage immune function has not been completely elucidated. We investigated the effects of CS extract...
Source: Cellular Signalling - May 27, 2021 Category: Cytology Authors: Kyoung-Hee Lee Jisu Woo Ji Yeon Kim Chang-Hoon Lee Chul-Gyu Yoo Source Type: research

Responses of porcupine and Wntless proteins to oxidative, hypoxic and endoplasmic reticulum stresses
Cell Signal. 2021 May 17:110047. doi: 10.1016/j.cellsig.2021.110047. Online ahead of print.ABSTRACTThe WNT (Wingless and Int-1) proteins play a role in stem cell development and cell differentiation. Mutations in the WNT proteins lead to the development of various tumours, including gastric tumours. Porcupine (PORCN) is a palmitoyltransferase and Wntless (WLS) is a chaperone protein that modify and fold the WNT proteins respectively and are involved in their proper secretion and binding to the frizzled (FZD) receptor and the lipoprotein receptor-related protein 5 or 6 (LRP5/6). We investigated how modifications of PORCN an...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Rowida Mohamed Catherine Kennedy William G Willmore Source Type: research

Targeting integrated stress response regulates microglial M1/M2 polarization and attenuates neuroinflammation following surgical brain injury in rat
Cell Signal. 2021 May 17;85:110048. doi: 10.1016/j.cellsig.2021.110048. Online ahead of print.ABSTRACTIntegrated stress response (ISR) contributes to various neuropathological processes and acting as a therapy target in CNS injuries. However, the fundamental role of ISR in regulating microglial polarization remains largely unknown. Currently no proper pharmacological approaches to reverse microglia-driven neuroinflammation in surgical brain injury (SBI) have been reported. Here we found that inhibition of the crucial ISR effector, activating transcription factor 4 (ATF4), using the RNA interference suppressed the lipopolys...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Teng-Chao Huang Lun Luo Shi-Hai Jiang Chuan Chen Hai-Yong He Chao-Feng Liang Wen-Sheng Li Hui Wang Lei Zhu Kun Wang Ying Guo Source Type: research

Responses of porcupine and Wntless proteins to oxidative, hypoxic and endoplasmic reticulum stresses
Cell Signal. 2021 May 17:110047. doi: 10.1016/j.cellsig.2021.110047. Online ahead of print.ABSTRACTThe WNT (Wingless and Int-1) proteins play a role in stem cell development and cell differentiation. Mutations in the WNT proteins lead to the development of various tumours, including gastric tumours. Porcupine (PORCN) is a palmitoyltransferase and Wntless (WLS) is a chaperone protein that modify and fold the WNT proteins respectively and are involved in their proper secretion and binding to the frizzled (FZD) receptor and the lipoprotein receptor-related protein 5 or 6 (LRP5/6). We investigated how modifications of PORCN an...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Rowida Mohamed Catherine Kennedy William G Willmore Source Type: research

Targeting integrated stress response regulates microglial M1/M2 polarization and attenuates neuroinflammation following surgical brain injury in rat
Cell Signal. 2021 May 17;85:110048. doi: 10.1016/j.cellsig.2021.110048. Online ahead of print.ABSTRACTIntegrated stress response (ISR) contributes to various neuropathological processes and acting as a therapy target in CNS injuries. However, the fundamental role of ISR in regulating microglial polarization remains largely unknown. Currently no proper pharmacological approaches to reverse microglia-driven neuroinflammation in surgical brain injury (SBI) have been reported. Here we found that inhibition of the crucial ISR effector, activating transcription factor 4 (ATF4), using the RNA interference suppressed the lipopolys...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Teng-Chao Huang Lun Luo Shi-Hai Jiang Chuan Chen Hai-Yong He Chao-Feng Liang Wen-Sheng Li Hui Wang Lei Zhu Kun Wang Ying Guo Source Type: research

Responses of porcupine and Wntless proteins to oxidative, hypoxic and endoplasmic reticulum stresses
Cell Signal. 2021 May 17:110047. doi: 10.1016/j.cellsig.2021.110047. Online ahead of print.ABSTRACTThe WNT (Wingless and Int-1) proteins play a role in stem cell development and cell differentiation. Mutations in the WNT proteins lead to the development of various tumours, including gastric tumours. Porcupine (PORCN) is a palmitoyltransferase and Wntless (WLS) is a chaperone protein that modify and fold the WNT proteins respectively and are involved in their proper secretion and binding to the frizzled (FZD) receptor and the lipoprotein receptor-related protein 5 or 6 (LRP5/6). We investigated how modifications of PORCN an...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Rowida Mohamed Catherine Kennedy William G Willmore Source Type: research

Targeting integrated stress response regulates microglial M1/M2 polarization and attenuates neuroinflammation following surgical brain injury in rat
Cell Signal. 2021 May 17;85:110048. doi: 10.1016/j.cellsig.2021.110048. Online ahead of print.ABSTRACTIntegrated stress response (ISR) contributes to various neuropathological processes and acting as a therapy target in CNS injuries. However, the fundamental role of ISR in regulating microglial polarization remains largely unknown. Currently no proper pharmacological approaches to reverse microglia-driven neuroinflammation in surgical brain injury (SBI) have been reported. Here we found that inhibition of the crucial ISR effector, activating transcription factor 4 (ATF4), using the RNA interference suppressed the lipopolys...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Teng-Chao Huang Lun Luo Shi-Hai Jiang Chuan Chen Hai-Yong He Chao-Feng Liang Wen-Sheng Li Hui Wang Lei Zhu Kun Wang Ying Guo Source Type: research

Responses of porcupine and Wntless proteins to oxidative, hypoxic and endoplasmic reticulum stresses
Cell Signal. 2021 May 17:110047. doi: 10.1016/j.cellsig.2021.110047. Online ahead of print.ABSTRACTThe WNT (Wingless and Int-1) proteins play a role in stem cell development and cell differentiation. Mutations in the WNT proteins lead to the development of various tumours, including gastric tumours. Porcupine (PORCN) is a palmitoyltransferase and Wntless (WLS) is a chaperone protein that modify and fold the WNT proteins respectively and are involved in their proper secretion and binding to the frizzled (FZD) receptor and the lipoprotein receptor-related protein 5 or 6 (LRP5/6). We investigated how modifications of PORCN an...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Rowida Mohamed Catherine Kennedy William G Willmore Source Type: research

Targeting integrated stress response regulates microglial M1/M2 polarization and attenuates neuroinflammation following surgical brain injury in rat
Cell Signal. 2021 May 17:110048. doi: 10.1016/j.cellsig.2021.110048. Online ahead of print.ABSTRACTIntegrated stress response (ISR) contributes to various neuropathological processes and acting as a therapy target in CNS injuries. However, the fundamental role of ISR in regulating microglial polarization remains largely unknown. Currently no proper pharmacological approaches to reverse microglia-driven neuroinflammation in surgical brain injury (SBI) have been reported. Here we found that inhibition of the crucial ISR effector, activating transcription factor 4 (ATF4), using the RNA interference suppressed the lipopolysacc...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Teng-Chao Huang Lun Luo Shi-Hai Jiang Chuan Chen Hai-Yong He Chao-Feng Liang Wen-Sheng Li Hui Wang Lei Zhu Kun Wang Ying Guo Source Type: research

Responses of porcupine and Wntless proteins to oxidative, hypoxic and endoplasmic reticulum stresses
Cell Signal. 2021 May 17:110047. doi: 10.1016/j.cellsig.2021.110047. Online ahead of print.ABSTRACTThe WNT (Wingless and Int-1) proteins play a role in stem cell development and cell differentiation. Mutations in the WNT proteins lead to the development of various tumours, including gastric tumours. Porcupine (PORCN) is a palmitoyltransferase and Wntless (WLS) is a chaperone protein that modify and fold the WNT proteins respectively and are involved in their proper secretion and binding to the frizzled (FZD) receptor and the lipoprotein receptor-related protein 5 or 6 (LRP5/6). We investigated how modifications of PORCN an...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Rowida Mohamed Catherine Kennedy William G Willmore Source Type: research

Targeting integrated stress response regulates microglial M1/M2 polarization and attenuates neuroinflammation following surgical brain injury in rat
Cell Signal. 2021 May 17:110048. doi: 10.1016/j.cellsig.2021.110048. Online ahead of print.ABSTRACTIntegrated stress response (ISR) contributes to various neuropathological processes and acting as a therapy target in CNS injuries. However, the fundamental role of ISR in regulating microglial polarization remains largely unknown. Currently no proper pharmacological approaches to reverse microglia-driven neuroinflammation in surgical brain injury (SBI) have been reported. Here we found that inhibition of the crucial ISR effector, activating transcription factor 4 (ATF4), using the RNA interference suppressed the lipopolysacc...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Teng-Chao Huang Lun Luo Shi-Hai Jiang Chuan Chen Hai-Yong He Chao-Feng Liang Wen-Sheng Li Hui Wang Lei Zhu Kun Wang Ying Guo Source Type: research

Responses of porcupine and Wntless proteins to oxidative, hypoxic and endoplasmic reticulum stresses
Cell Signal. 2021 May 17:110047. doi: 10.1016/j.cellsig.2021.110047. Online ahead of print.ABSTRACTThe WNT (Wingless and Int-1) proteins play a role in stem cell development and cell differentiation. Mutations in the WNT proteins lead to the development of various tumours, including gastric tumours. Porcupine (PORCN) is a palmitoyltransferase and Wntless (WLS) is a chaperone protein that modify and fold the WNT proteins respectively and are involved in their proper secretion and binding to the frizzled (FZD) receptor and the lipoprotein receptor-related protein 5 or 6 (LRP5/6). We investigated how modifications of PORCN an...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Rowida Mohamed Catherine Kennedy William G Willmore Source Type: research

Targeting integrated stress response regulates microglial M1/M2 polarization and attenuates neuroinflammation following surgical brain injury in rat
Cell Signal. 2021 May 17:110048. doi: 10.1016/j.cellsig.2021.110048. Online ahead of print.ABSTRACTIntegrated stress response (ISR) contributes to various neuropathological processes and acting as a therapy target in CNS injuries. However, the fundamental role of ISR in regulating microglial polarization remains largely unknown. Currently no proper pharmacological approaches to reverse microglia-driven neuroinflammation in surgical brain injury (SBI) have been reported. Here we found that inhibition of the crucial ISR effector, activating transcription factor 4 (ATF4), using the RNA interference suppressed the lipopolysacc...
Source: Cellular Signalling - May 20, 2021 Category: Cytology Authors: Teng-Chao Huang Lun Luo Shi-Hai Jiang Chuan Chen Hai-Yong He Chao-Feng Liang Wen-Sheng Li Hui Wang Lei Zhu Kun Wang Ying Guo Source Type: research

Focal adhesion dynamics in cellular function and disease
Cell Signal. 2021 May 15:110046. doi: 10.1016/j.cellsig.2021.110046. Online ahead of print.ABSTRACTActing as a bridge between the cytoskeleton of the cell and the extra cellular matrix (ECM), the cell-ECM adhesions with integrins at their core, play a major role in cell signalling to direct mechanotransduction, cell migration, cell cycle progression, proliferation, differentiation, growth and repair. Biochemically, these adhesions are composed of diverse, yet an organised group of structural proteins, receptors, adaptors, various enzymes including protein kinases, phosphatases, GTPases, proteases, etc. as well as scaffoldi...
Source: Cellular Signalling - May 18, 2021 Category: Cytology Authors: Yasaswi Gayatri Mishra Bramanandam Manavathi Source Type: research

Focal adhesion dynamics in cellular function and disease
Cell Signal. 2021 May 15:110046. doi: 10.1016/j.cellsig.2021.110046. Online ahead of print.ABSTRACTActing as a bridge between the cytoskeleton of the cell and the extra cellular matrix (ECM), the cell-ECM adhesions with integrins at their core, play a major role in cell signalling to direct mechanotransduction, cell migration, cell cycle progression, proliferation, differentiation, growth and repair. Biochemically, these adhesions are composed of diverse, yet an organised group of structural proteins, receptors, adaptors, various enzymes including protein kinases, phosphatases, GTPases, proteases, etc. as well as scaffoldi...
Source: Cellular Signalling - May 18, 2021 Category: Cytology Authors: Yasaswi Gayatri Mishra Bramanandam Manavathi Source Type: research

HSP47 contributes to angiogenesis by induction of CCL2 in bladder cancer
In this study, we report a novel mechanism of HSP47-induced angiogenesis in bladder cancer (BC). We find that HSP47 is abnormally overexpressed in BC and is correlated with poor prognosis. HSP47 down-regulation suppresses angiogenesis in BC cells. Mechanistically, activation of the ERK pathway and induction of C-C Motif Chemokine Ligand 2 (CCL2) are responsible for HSP47-induced angiogenesis. The correlation between HSP47 with CCL2 and angiogenesis is further confirmed in BC clinical samples. Taken together, our findings suggest that HSP47 contributes to BC angiogenesis by induction of CCL2 and provide a potential anti-ang...
Source: Cellular Signalling - May 17, 2021 Category: Cytology Authors: Wenlong Ma Tong Ou Xiangrui Cui Kai Wu Hongming Li Yuqing Li Guoyu Peng Wuchao Xia Song Wu Source Type: research

Limb-bud and heart (LBH) inhibits cellular migration, invasion and epithelial-mesenchymal transition in nasopharyngeal carcinoma via downregulating αB-crystallin expression
Cell Signal. 2021 May 14:110045. doi: 10.1016/j.cellsig.2021.110045. Online ahead of print.ABSTRACTLimb-bud and heart (LBH) gene has received increasing attention in recent cancer studies. Here we investigated the role of the LBH gene in regulating the metastasis capacity and epithelial-mesenchymal transition (EMT) of nasopharyngeal carcinoma (NPC) cells, and its potential mechanism. The expressions of LBH and αB-crystallin (CRYAB) were modulated by lentiviral infection, or plasmid/siRNA transfection, and the phosphorylation of p38 was suppressed by an inhibitor, to explore their functions in modulating NPC cell phen...
Source: Cellular Signalling - May 17, 2021 Category: Cytology Authors: Anbiao Wu Ling Zhang Lihong Zhang Li Li Qicai Liu Source Type: research

p62/Sequestosome 1 regulates transforming growth factor beta signaling and epithelial to mesenchymal transition in A549 cells
Cell Signal. 2021 May 14:110040. doi: 10.1016/j.cellsig.2021.110040. Online ahead of print.ABSTRACTTransforming growth factor beta (TGFβ) receptor trafficking regulates many TGFβ-dependent cellular outcomes including epithelial to mesenchymal transition (EMT). EMT in A549 non-small cell lung cancer (NSCLC) cells has recently been linked to the regulation of cellular autophagy. Here, we investigated the role of the autophagy cargo receptor, p62/sequestosome 1 (SQSTM1), in regulating TGFβ receptor trafficking, TGFβ1-dependent Smad2 phosphorylation and EMT in A549 NSCLC cells. Using immunofluorescence micr...
Source: Cellular Signalling - May 17, 2021 Category: Cytology Authors: Charles B Trelford Evelyn Ng Craig I Campbell Gianni M Di Guglielmo Source Type: research

HSP47 contributes to angiogenesis by induction of CCL2 in bladder cancer
In this study, we report a novel mechanism of HSP47-induced angiogenesis in bladder cancer (BC). We find that HSP47 is abnormally overexpressed in BC and is correlated with poor prognosis. HSP47 down-regulation suppresses angiogenesis in BC cells. Mechanistically, activation of the ERK pathway and induction of C-C Motif Chemokine Ligand 2 (CCL2) are responsible for HSP47-induced angiogenesis. The correlation between HSP47 with CCL2 and angiogenesis is further confirmed in BC clinical samples. Taken together, our findings suggest that HSP47 contributes to BC angiogenesis by induction of CCL2 and provide a potential anti-ang...
Source: Cellular Signalling - May 17, 2021 Category: Cytology Authors: Wenlong Ma Tong Ou Xiangrui Cui Kai Wu Hongming Li Yuqing Li Guoyu Peng Wuchao Xia Song Wu Source Type: research

Limb-bud and heart (LBH) inhibits cellular migration, invasion and epithelial-mesenchymal transition in nasopharyngeal carcinoma via downregulating αB-crystallin expression
Cell Signal. 2021 May 14:110045. doi: 10.1016/j.cellsig.2021.110045. Online ahead of print.ABSTRACTLimb-bud and heart (LBH) gene has received increasing attention in recent cancer studies. Here we investigated the role of the LBH gene in regulating the metastasis capacity and epithelial-mesenchymal transition (EMT) of nasopharyngeal carcinoma (NPC) cells, and its potential mechanism. The expressions of LBH and αB-crystallin (CRYAB) were modulated by lentiviral infection, or plasmid/siRNA transfection, and the phosphorylation of p38 was suppressed by an inhibitor, to explore their functions in modulating NPC cell phen...
Source: Cellular Signalling - May 17, 2021 Category: Cytology Authors: Anbiao Wu Ling Zhang Lihong Zhang Li Li Qicai Liu Source Type: research

p62/Sequestosome 1 regulates transforming growth factor beta signaling and epithelial to mesenchymal transition in A549 cells
Cell Signal. 2021 May 14:110040. doi: 10.1016/j.cellsig.2021.110040. Online ahead of print.ABSTRACTTransforming growth factor beta (TGFβ) receptor trafficking regulates many TGFβ-dependent cellular outcomes including epithelial to mesenchymal transition (EMT). EMT in A549 non-small cell lung cancer (NSCLC) cells has recently been linked to the regulation of cellular autophagy. Here, we investigated the role of the autophagy cargo receptor, p62/sequestosome 1 (SQSTM1), in regulating TGFβ receptor trafficking, TGFβ1-dependent Smad2 phosphorylation and EMT in A549 NSCLC cells. Using immunofluorescence micr...
Source: Cellular Signalling - May 17, 2021 Category: Cytology Authors: Charles B Trelford Evelyn Ng Craig I Campbell Gianni M Di Guglielmo Source Type: research

CTRP3 promotes TNF- α-induced apoptosis and barrier dysfunction in salivary epithelial cells
CONCLUSIONS: These findings suggest that the salivary glands are a novel source of CTRP3 synthesis and secretion. CTRP3 might promote TNF-α-induced cell apoptosis through the TNFR1-mediated complex II pathway.PMID:33991612 | DOI:10.1016/j.cellsig.2021.110042 (Source: Cellular Signalling)
Source: Cellular Signalling - May 15, 2021 Category: Cytology Authors: Mei Mei Ling-Han Qu Xin Cong Yan Zhang Ruo-Lan Xiang Guang-Yan Yu Jia-Zeng Su Li-Ling Wu Source Type: research

The impact of Caspase-1 deletion on apoptosis and acute kidney injury in a murine transplant model
CONCLUSIONS: Our data suggest that other triggers of inflammation and programmed necrosis may need to be inhibited in addition to attenuating Caspase-1 to fully prevent AKI after kidney transplant. Importantly, requirements may be distinct for AKI induced by transplantation as opposed to other transient models such as the clamp model of AKI.PMID:33991613 | DOI:10.1016/j.cellsig.2021.110039 (Source: Cellular Signalling)
Source: Cellular Signalling - May 15, 2021 Category: Cytology Authors: Swati Jain Robert Plenter Rahkola Jeremy Trevor Nydam Ronald G Gill Alkesh Jani Source Type: research

Physiological and pathological functions of sphingolipids in pregnancy
Cell Signal. 2021 May 12:110041. doi: 10.1016/j.cellsig.2021.110041. Online ahead of print.ABSTRACTSignaling by the bioactive sphingolipid, sphingosine 1-phosphate (S1P), and its precursors during pregnancy are emerging areas in pregnancy research. S1P and ceramide levels increase towards end of gestation, suggesting a physiological role in parturition. However, high levels of circulating S1P and ceramide are correlated with pregnancy disorders such as preeclampsia, gestational diabetes mellitus and intrauterine growth restriction. Expression of placental and decidual enzymes that metabolize S1P and S1P receptors are also ...
Source: Cellular Signalling - May 15, 2021 Category: Cytology Authors: Yuliya Fakhr David N Brindley Denise G Hemmings Source Type: research

Secretory Galectin-3 promotes hepatic steatosis via regulation of the PPAR γ/CD36 signaling pathway
In conclusion, the present study confirmed the roles of Gal3 in hepatic lipid metabolism in both in vitro and in vivo studies and revealed that Gal3 is a secretory protein that promotes hepatic steatosis through the PPARγ-CD36-dependent pathway, suggesting that targeting Gal3 may represent a potential therapeutic approach for the treatment of NAFLD and related metabolic disorders.PMID:33991615 | DOI:10.1016/j.cellsig.2021.110043 (Source: Cellular Signalling)
Source: Cellular Signalling - May 15, 2021 Category: Cytology Authors: Huiyuan Yu Fan Yang Wentao Zhong Xin Jiang Fan Zhang Xueying Ji Mengjuan Xue Yixuan Qiu Jiaming Yu Xiaona Hu Jie Chen Zhijun Bao Source Type: research

CTRP3 promotes TNF- α-induced apoptosis and barrier dysfunction in salivary epithelial cells
CONCLUSIONS: These findings suggest that the salivary glands are a novel source of CTRP3 synthesis and secretion. CTRP3 might promote TNF-α-induced cell apoptosis through the TNFR1-mediated complex II pathway.PMID:33991612 | DOI:10.1016/j.cellsig.2021.110042 (Source: Cellular Signalling)
Source: Cellular Signalling - May 15, 2021 Category: Cytology Authors: Mei Mei Ling-Han Qu Xin Cong Yan Zhang Ruo-Lan Xiang Guang-Yan Yu Jia-Zeng Su Li-Ling Wu Source Type: research

The impact of Caspase-1 deletion on apoptosis and acute kidney injury in a murine transplant model
CONCLUSIONS: Our data suggest that other triggers of inflammation and programmed necrosis may need to be inhibited in addition to attenuating Caspase-1 to fully prevent AKI after kidney transplant. Importantly, requirements may be distinct for AKI induced by transplantation as opposed to other transient models such as the clamp model of AKI.PMID:33991613 | DOI:10.1016/j.cellsig.2021.110039 (Source: Cellular Signalling)
Source: Cellular Signalling - May 15, 2021 Category: Cytology Authors: Swati Jain Robert Plenter Rahkola Jeremy Trevor Nydam Ronald G Gill Alkesh Jani Source Type: research

Physiological and pathological functions of sphingolipids in pregnancy
Cell Signal. 2021 May 12:110041. doi: 10.1016/j.cellsig.2021.110041. Online ahead of print.ABSTRACTSignaling by the bioactive sphingolipid, sphingosine 1-phosphate (S1P), and its precursors during pregnancy are emerging areas in pregnancy research. S1P and ceramide levels increase towards end of gestation, suggesting a physiological role in parturition. However, high levels of circulating S1P and ceramide are correlated with pregnancy disorders such as preeclampsia, gestational diabetes mellitus and intrauterine growth restriction. Expression of placental and decidual enzymes that metabolize S1P and S1P receptors are also ...
Source: Cellular Signalling - May 15, 2021 Category: Cytology Authors: Yuliya Fakhr David N Brindley Denise G Hemmings Source Type: research

Secretory Galectin-3 promotes hepatic steatosis via regulation of the PPAR γ/CD36 signaling pathway
In conclusion, the present study confirmed the roles of Gal3 in hepatic lipid metabolism in both in vitro and in vivo studies and revealed that Gal3 is a secretory protein that promotes hepatic steatosis through the PPARγ-CD36-dependent pathway, suggesting that targeting Gal3 may represent a potential therapeutic approach for the treatment of NAFLD and related metabolic disorders.PMID:33991615 | DOI:10.1016/j.cellsig.2021.110043 (Source: Cellular Signalling)
Source: Cellular Signalling - May 15, 2021 Category: Cytology Authors: Huiyuan Yu Fan Yang Wentao Zhong Xin Jiang Fan Zhang Xueying Ji Mengjuan Xue Yixuan Qiu Jiaming Yu Xiaona Hu Jie Chen Zhijun Bao Source Type: research

AKR1C3 decreased CML sensitivity to Imatinib in bone marrow microenvironment via dysregulation of miR-379-5p
CONCLUSIONS: Altogether, our study identifies a novel signaling regulation of miR-379-5p/AKR1C3/EKR axis in regulating IM resistance in CML cell, and provides a scientific base for exploring AKR1C3 as a biomarker in impeding IM resistance in CML.PMID:33984486 | DOI:10.1016/j.cellsig.2021.110038 (Source: Cellular Signalling)
Source: Cellular Signalling - May 13, 2021 Category: Cytology Authors: Di Pan Wanwan Yang Yao Zeng Wenjun Li Kaizhen Wang Li Zhao Jia Li Yuting Ye Qinglong Guo Source Type: research

AKR1C3 decreased CML sensitivity to Imatinib in bone marrow microenvironment via dysregulation of miR-379-5p
CONCLUSIONS: Altogether, our study identifies a novel signaling regulation of miR-379-5p/AKR1C3/EKR axis in regulating IM resistance in CML cell, and provides a scientific base for exploring AKR1C3 as a biomarker in impeding IM resistance in CML.PMID:33984486 | DOI:10.1016/j.cellsig.2021.110038 (Source: Cellular Signalling)
Source: Cellular Signalling - May 13, 2021 Category: Cytology Authors: Di Pan Wanwan Yang Yao Zeng Wenjun Li Kaizhen Wang Li Zhao Jia Li Yuting Ye Qinglong Guo Source Type: research