BRD4L cooperates with MYC to block local tumor invasion via suppression of S100A10
In this study, we employ a systematic approach to delete exons that encode the low-complexity domain (LCD) of BRD4L in cells by using CRISPR-Cas9. In particular, the deletion of exon 14 (BRD4-E14) results in cellular morphological changes towards spindle-shaped and loosely packed. BRD4-E14 deficient cells show increased cell migration and reduced cell adhesion. The expression of S100A10 was significantly increased in cells lacking E14. BRD4L binds with MYC via the E14-encoded region of the LCD to inhibit the expression of S100A10. In cancer tissues, there is a positive correlation between BRD4 and MYC, while both of these ...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Yongyi Ma Nan Liu Yu Shi Shuyan Ma Yingjun Wang Wen Zheng Rong Sun Yihua Song Miaomiao Chen Lishuai Qu Renfang Mao Yihui Fan Source Type: research
Cadmium contributes to cardiac metabolic disruption by activating endothelial HIF1A-GLUT1 axis
In this study, male C57BL/6 J mice were exposed to cadmium chloride (CdCl2) through drinking water for 4 weeks. We found that exposure to CdCl2 increased glucose uptake and utilization, and disrupted normal metabolisms in the heart. In vitro studies showed that CdCl2 specifically increased endothelial glucose uptake without affecting cardiomyocytic glucose uptake and endothelial fatty acid uptake. The glucose transporter 1 (GLUT1) as well as its transcription factor HIF1A was significantly increased after CdCl2 treatment in endothelial cells. Further investigations found that CdCl2 treatment upregulated HIF1A expression by...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Xiaoyu Zhang Wendan Zheng Shiyu Sun Yang Du Wenjuan Xu Zongguo Sun Fuhong Liu Manzhi Wang Zuohui Zhao Ju Liu Qiang Liu Source Type: research
Upregulated miR-374a-5p drives psoriasis pathogenesis through WIF1 downregulation and Wnt5a/NF- κB activation
CONCLUSION: Our findings elucidate the role of miR-374a-5p upregulation in the pathogenesis of psoriasis through inhibition of WIF1 and activation of the Wnt5a/NF-κB pathway, providing new potential therapeutic targets for psoriasis.PMID:38604345 | DOI:10.1016/j.cellsig.2024.111171 (Source: Cellular Signalling)
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Jing Ma Lu Gan Hongying Chen Lihao Chen Yu Hu Chao Luan Kun Chen Jiaan Zhang Source Type: research
Calix[6]arene dismantles extracellular vesicle biogenesis and metalloproteinases that support pancreatic cancer hallmarks
Cell Signal. 2024 Apr 9:111174. doi: 10.1016/j.cellsig.2024.111174. Online ahead of print.ABSTRACTMany challenges are faced in pancreatic cancer treatment due to late diagnosis and poor prognosis because of high recurrence and metastasis. Extracellular vesicles (EVs) and matrix metalloproteinases (MMPs), besides acting in intercellular communication, are key players in the cancer cell plasticity responsible for initiating metastasis. Therefore, these entities provide valuable targets for the development of better treatments. In this context, this study aimed to evaluate the potential of calix[6]arene to disturb the release...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Helon Guimar ães Cordeiro Jordana Maria Azevedo-Martins Alessandra Val éria de Sousa Faria Karin Juliane Pelizzaro Rocha-Brito Renato Milani Maikel Peppelenbosch Gwenny Fuhler Ângelo de Fátima Carmen Ver íssima Ferreira-Halder Source Type: research
Transient stimulation of TRPMLs enhance the functionality of hDPCs and facilitate hair growth in mice
Cell Signal. 2024 Apr 9:111167. doi: 10.1016/j.cellsig.2024.111167. Online ahead of print.ABSTRACTAutophagy is essential for eliminating aging and organelle damage that maintaining cellular homeostasis. However, the dysfunction of autophagy has been proven in hair loss such as AGA. Despite the crucial role of TRPML channels in regulating autophagy, their specific function in hair growth remains unclarified. To investigate the biological functions and associated molecular mechanisms of TRPMLs in hair growth, Animal experiments were conducted to confirm the function of TRLMLs activation in promoting hair growth. Subsequently...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Jiayi Sun Hongyan Zhang Bo Xie Yuqing Shen Yuqi Zhu Wen Xu Beilei Zhang Xiuzu Song Source Type: research
Simvastatin activates the spindle assembly checkpoint and causes abnormal cell division by modifying small GTPases
In this study, we determined its effect on cell division. Cell cycle synchronization experiments revealed a delay in mitotic progression in simvastatin-treated cells at concentrations lower than the IC50. Time-lapse imaging analysis indicated that the duration of mitosis, especially from mitotic entry to anaphase onset, was prolonged. In addition, simvastatin increased the number of cells exhibiting misoriented anaphase/telophase and bleb formation. Inhibition of the spindle assembly checkpoint (SAC) kinase Mps1 canceled the mitotic delay. Additionally, the number of cells exhibiting kinetochore localization of BubR1, an e...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Junna Tanaka Hiroki Kuwajima Ryuzaburo Yuki Yuji Nakayama Source Type: research
BRD4L cooperates with MYC to block local tumor invasion via suppression of S100A10
In this study, we employ a systematic approach to delete exons that encode the low-complexity domain (LCD) of BRD4L in cells by using CRISPR-Cas9. In particular, the deletion of exon 14 (BRD4-E14) results in cellular morphological changes towards spindle-shaped and loosely packed. BRD4-E14 deficient cells show increased cell migration and reduced cell adhesion. The expression of S100A10 was significantly increased in cells lacking E14. BRD4L binds with MYC via the E14-encoded region of the LCD to inhibit the expression of S100A10. In cancer tissues, there is a positive correlation between BRD4 and MYC, while both of these ...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Yongyi Ma Nan Liu Yu Shi Shuyan Ma Yingjun Wang Wen Zheng Rong Sun Yihua Song Miaomiao Chen Lishuai Qu Renfang Mao Yihui Fan Source Type: research
Cadmium contributes to cardiac metabolic disruption by activating endothelial HIF1A-GLUT1 axis
In this study, male C57BL/6 J mice were exposed to cadmium chloride (CdCl2) through drinking water for 4 weeks. We found that exposure to CdCl2 increased glucose uptake and utilization, and disrupted normal metabolisms in the heart. In vitro studies showed that CdCl2 specifically increased endothelial glucose uptake without affecting cardiomyocytic glucose uptake and endothelial fatty acid uptake. The glucose transporter 1 (GLUT1) as well as its transcription factor HIF1A was significantly increased after CdCl2 treatment in endothelial cells. Further investigations found that CdCl2 treatment upregulated HIF1A expression by...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Xiaoyu Zhang Wendan Zheng Shiyu Sun Yang Du Wenjuan Xu Zongguo Sun Fuhong Liu Manzhi Wang Zuohui Zhao Ju Liu Qiang Liu Source Type: research
Upregulated miR-374a-5p drives psoriasis pathogenesis through WIF1 downregulation and Wnt5a/NF- κB activation
CONCLUSION: Our findings elucidate the role of miR-374a-5p upregulation in the pathogenesis of psoriasis through inhibition of WIF1 and activation of the Wnt5a/NF-κB pathway, providing new potential therapeutic targets for psoriasis.PMID:38604345 | DOI:10.1016/j.cellsig.2024.111171 (Source: Cellular Signalling)
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Jing Ma Lu Gan Hongying Chen Lihao Chen Yu Hu Chao Luan Kun Chen Jiaan Zhang Source Type: research
Calix[6]arene dismantles extracellular vesicle biogenesis and metalloproteinases that support pancreatic cancer hallmarks
Cell Signal. 2024 Apr 9:111174. doi: 10.1016/j.cellsig.2024.111174. Online ahead of print.ABSTRACTMany challenges are faced in pancreatic cancer treatment due to late diagnosis and poor prognosis because of high recurrence and metastasis. Extracellular vesicles (EVs) and matrix metalloproteinases (MMPs), besides acting in intercellular communication, are key players in the cancer cell plasticity responsible for initiating metastasis. Therefore, these entities provide valuable targets for the development of better treatments. In this context, this study aimed to evaluate the potential of calix[6]arene to disturb the release...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Helon Guimar ães Cordeiro Jordana Maria Azevedo-Martins Alessandra Val éria de Sousa Faria Karin Juliane Pelizzaro Rocha-Brito Renato Milani Maikel Peppelenbosch Gwenny Fuhler Ângelo de Fátima Carmen Ver íssima Ferreira-Halder Source Type: research
Transient stimulation of TRPMLs enhance the functionality of hDPCs and facilitate hair growth in mice
Cell Signal. 2024 Apr 9:111167. doi: 10.1016/j.cellsig.2024.111167. Online ahead of print.ABSTRACTAutophagy is essential for eliminating aging and organelle damage that maintaining cellular homeostasis. However, the dysfunction of autophagy has been proven in hair loss such as AGA. Despite the crucial role of TRPML channels in regulating autophagy, their specific function in hair growth remains unclarified. To investigate the biological functions and associated molecular mechanisms of TRPMLs in hair growth, Animal experiments were conducted to confirm the function of TRLMLs activation in promoting hair growth. Subsequently...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Jiayi Sun Hongyan Zhang Bo Xie Yuqing Shen Yuqi Zhu Wen Xu Beilei Zhang Xiuzu Song Source Type: research
Simvastatin activates the spindle assembly checkpoint and causes abnormal cell division by modifying small GTPases
In this study, we determined its effect on cell division. Cell cycle synchronization experiments revealed a delay in mitotic progression in simvastatin-treated cells at concentrations lower than the IC50. Time-lapse imaging analysis indicated that the duration of mitosis, especially from mitotic entry to anaphase onset, was prolonged. In addition, simvastatin increased the number of cells exhibiting misoriented anaphase/telophase and bleb formation. Inhibition of the spindle assembly checkpoint (SAC) kinase Mps1 canceled the mitotic delay. Additionally, the number of cells exhibiting kinetochore localization of BubR1, an e...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Junna Tanaka Hiroki Kuwajima Ryuzaburo Yuki Yuji Nakayama Source Type: research
BRD4L cooperates with MYC to block local tumor invasion via suppression of S100A10
In this study, we employ a systematic approach to delete exons that encode the low-complexity domain (LCD) of BRD4L in cells by using CRISPR-Cas9. In particular, the deletion of exon 14 (BRD4-E14) results in cellular morphological changes towards spindle-shaped and loosely packed. BRD4-E14 deficient cells show increased cell migration and reduced cell adhesion. The expression of S100A10 was significantly increased in cells lacking E14. BRD4L binds with MYC via the E14-encoded region of the LCD to inhibit the expression of S100A10. In cancer tissues, there is a positive correlation between BRD4 and MYC, while both of these ...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Yongyi Ma Nan Liu Yu Shi Shuyan Ma Yingjun Wang Wen Zheng Rong Sun Yihua Song Miaomiao Chen Lishuai Qu Renfang Mao Yihui Fan Source Type: research
Cadmium contributes to cardiac metabolic disruption by activating endothelial HIF1A-GLUT1 axis
In this study, male C57BL/6 J mice were exposed to cadmium chloride (CdCl2) through drinking water for 4 weeks. We found that exposure to CdCl2 increased glucose uptake and utilization, and disrupted normal metabolisms in the heart. In vitro studies showed that CdCl2 specifically increased endothelial glucose uptake without affecting cardiomyocytic glucose uptake and endothelial fatty acid uptake. The glucose transporter 1 (GLUT1) as well as its transcription factor HIF1A was significantly increased after CdCl2 treatment in endothelial cells. Further investigations found that CdCl2 treatment upregulated HIF1A expression by...
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Xiaoyu Zhang Wendan Zheng Shiyu Sun Yang Du Wenjuan Xu Zongguo Sun Fuhong Liu Manzhi Wang Zuohui Zhao Ju Liu Qiang Liu Source Type: research
Upregulated miR-374a-5p drives psoriasis pathogenesis through WIF1 downregulation and Wnt5a/NF- κB activation
CONCLUSION: Our findings elucidate the role of miR-374a-5p upregulation in the pathogenesis of psoriasis through inhibition of WIF1 and activation of the Wnt5a/NF-κB pathway, providing new potential therapeutic targets for psoriasis.PMID:38604345 | DOI:10.1016/j.cellsig.2024.111171 (Source: Cellular Signalling)
Source: Cellular Signalling - April 11, 2024 Category: Cytology Authors: Jing Ma Lu Gan Hongying Chen Lihao Chen Yu Hu Chao Luan Kun Chen Jiaan Zhang Source Type: research
