Query: siRNA

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Total 15120 results found since Jan 2013.

Endoplasmic reticulum stress triggered by Soyasapogenol B promotes apoptosis and autophagy in colorectal cancer
Publication date: Available online 14 December 2018Source: Life SciencesAuthor(s): Luping Wang, Lu Yun, Xiaojun Wang, Liying Sha, Luning Wang, Yingying Sui, Hui ZhangAbstractAimColorectal cancer (CRC) is a common human malignancy which accounts for 600,000 deaths annually at the global level. Soyasapogenol B (Soy B), an ingredient of soybean, has been found to exert anti-proliferative activities in vitro in human breast cancer cells. The current study aimed to evaluate the efficacy of Soy B in suppressing CRC.Methods and materialsThe effect of Soy B on cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay...
Source: Life Sciences - December 14, 2018 Category: Biology Source Type: research

Silencing the ACAT1 Gene in Human SH-SY5Y Neuroblastoma Cells Inhibits the Expression of Cyclo-Oxygenase 2 (COX2) and Reduces β-Amyloid-Induced Toxicity Due to Activation of Protein Kinase C (PKC) and ERK.
CONCLUSIONS Silencing ACAT1 attenuates Aβ₂₅₋₃₅-induced cytotoxicity and cell apoptosis in SH-SY5Y cells, which may due to the synergistic effect of ACAT1 and COX2 through PKC/ERK pathways. PMID: 30541014 [PubMed - in process]
Source: Medical Science Monitor - December 14, 2018 Category: Research Tags: Med Sci Monit Source Type: research

CIP2A Promotes Proliferation, Invasion and Chemoresistance to Cisplatin in Renal Cell Carcinoma
CIP2A is a well-known oncoprotein whose expression is elevated in multiple human solid tumor types. However, its role in renal cell carcinoma (RCC) development is poorly understood. Thus, in our present study, we used the renal cancer cell lines 786-O, A498 and CAKI-1 and the renal epithelial cell line HK-2 to clarify the function of CIP2A in RCC. We found that CIP2A expression is much higher in the RCC cells than in the normal renal epithelial cell. Lentivirus covered coding region CIP2A cDNA sequence and CIP2A siRNA were used to up and down regulate CIP2A expression in vitro. We found that overexpression of CIP2A promote...
Source: Journal of Cancer - December 14, 2018 Category: Cancer & Oncology Authors: Yongzhen Zhang, Liang Fang, Yuanwei Zang, Juchao Ren, Zhonghua Xu Tags: Research Paper Source Type: research

S100A4-MYH9 Axis Promote Migration and Invasion of Gastric Cancer Cells by Inducing TGF- β -Mediated Epithelial-Mesenchymal Transition
In conclusion, overexpression of S100A4 and downstream molecular MYH9 in advanced gastric cancer predicted poor prognosis; oncogene S100A4 facilitate EMT process induced by TGF-β stimulation, suggesting a potential target in management of peritoneal metastasis of gastric cancer.
Source: Journal of Cancer - December 14, 2018 Category: Cancer & Oncology Authors: Fengping Li, Jiaolong Shi, Zhijun Xu, Xingxing Yao, Tingyu Mou, Jiang Yu, Hao Liu, Guoxin Li Tags: Research Paper Source Type: research

Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells
In conclusion, this study showed the expression profiles of circRNAs regulated by HPV16 E7 in cervical cancer cells and provides novel insights into the new potential candidates for future mechanism studies.
Source: Journal of Cancer - December 14, 2018 Category: Cancer & Oncology Authors: Si-Rong Zheng, Han-Rong Zhang, Zhen-Fei Zhang, Shu-Yu Lai, Li-Jun Huang, Jie Liu, Xin Bai, Ke Ding, Jue-Yu Zhou Tags: Research Paper Source Type: research

Eukaryotic translation initiation factor 5A-2 involves in doxorubicin-induced epithelial-mesenchymal transition in oral squamous cell carcinoma cells
Conclusions: Our study strongly proved that combined treatment with GC7 may boost the therapeutic effect of doxorubicin in OSCC by inhibiting the EMT.
Source: Journal of Cancer - December 14, 2018 Category: Cancer & Oncology Authors: Liang Fang, Li Gao, Lei Xie, Guizhou Xiao Tags: Research Paper Source Type: research

PI3K/Akt/HIF-1 α signaling pathway mediates HPV-16 oncoprotein-induced expression of EMT-related transcription factors in non-small cell lung cancer cells
Conclusions: PI3K/Akt/HIF-1α may contribute to the progression of HPV-associated NSCLC via mediating the expression of EMT-related transcription factors in NSCLC cells.
Source: Journal of Cancer - December 14, 2018 Category: Cancer & Oncology Authors: Jinhua Liu, Bingyu Huang, Zihan Xiu, Zhiyuan Zhou, Jiao Liu, Xiangyong Li, Xudong Tang Tags: Research Paper Source Type: research

Stromal mesenchymal stem cells facilitate pancreatic cancer progression by regulating specific secretory molecules through mutual cellular interaction
Pancreatic ductal adenocarcinoma (PDAC) is currently one of the most intractable malignancies with a typical scirrhous pattern in histology. Due to its abundant tumor stroma and scant vascularization, chemotherapeutic agents are considered inefficiently permeable to cancer nests, making it highly difficult to cure the patients with PDAC. However, PDAC is also considered to owe its intractability to other critical factors such as cellular interaction between tumor cells and tumor microenvironment as well as architectural barriers, which increases in therapeutic resistance. Here, we report a specific cellular interaction bet...
Source: Journal of Cancer - December 14, 2018 Category: Cancer & Oncology Authors: Ken Saito, Masakiyo Sakaguchi, Satoshi Maruyama, Hidekazu Iioka, Endy Widya Putranto, I Wayan Sumardika, Nahoko Tomonobu, Takashi Kawasaki, Keiichi Homma, Eisaku Kondo Tags: Research Paper Source Type: research

Clinicopathological Significance and Antitumor Effect of MPHOSPH1 in Testicular Germ Cell Tumor
In conclusion, MPHOSPH1 may be a potential treatment option for TGCTs, and its expression may be a novel biomarker of poor prognosis.
Source: Journal of Cancer - December 14, 2018 Category: Cancer & Oncology Authors: Tatsuro Abe, Kenichi Kohashi, Junkichi Takemoto, Fumio Kinoshita, Masatoshi Eto, Yoshinao Oda Tags: Research Paper Source Type: research

GSE123820 RNA-seq data for non-targeting siRNA and lncRNA LOL siRNA transfection in MCF-7 cells
Contributors : Wei Sun ; Zhiming ShaoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo elucidate which portions of signaling pathways LOL influences in luminal breast cancer, RNA-seq analysis was performed in siLOL MCF-7 cell lines. Eight hundred eighty-three downregulated genes and 1182 upregulated genes (fold change ≥1.5) were detected after LOL knockdown. Gene set enrichment analysis (GSEA) of hallmarks indicated that the gene sets related to DNA_Repair, G2M_Checkpoint, E2F_Targets and MYC_Targets were positively correlated with LOL downregulation.
Source: GEO: Gene Expression Omnibus - December 14, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE114127 Compare the knock-down effects of SLC2A11-MIF and two parent genes in Hela cell line
Contributors : Peng Wu ; Fujun Qin ; Hui LiSeries Type : Expression profiling by arrayOrganism : Homo sapiensTotal RNA were extracted from five knocking down treated Hela cells using siRNA targeting fusion gene SLCC2A11-MIF and parental genesTo investigate the function of fusion gene and parental gene, we designed siRNAs specifically knocking down the fusion RNA SLC2A11-MIF and parental gene SLC2A11 respectively. Totally we have five samples. One sample is control, two siRNAs target parental genes and other two siRNAs target fusion genes. After 48hrs siRNA treatment, the RNAs were extracted from cells, and followed by RNA microarray.
Source: GEO: Gene Expression Omnibus - December 14, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE95057 Exploring the landscape of transcriptome 3 ’ end diversity (TREND) by applying TRENDseq
Conclusions: We analyzed the diversity of the transcriptome 3’end in response to depletion of 174 potential TREND regulators by RNAi. TRENDseq is capable to deconvolute the dynamics of TREND from highly multiplexed libraries. The screening revealed regulators of various l evels of gene expression control (e.g. transcription, splicing, mRNA turnover, etc.) to play an important role in the diversification of the transcriptome 3’ end, affecting over 3600 genes altogether. Data types: 1 – 198 siRNA depletion experiments including 174 knockdowns of putative TREND r egulators and 24 mock control samples. 2 – Biological...
Source: GEO: Gene Expression Omnibus - December 14, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Other Homo sapiens Source Type: research

Mediatory role of BLT2 in the proliferation of KRAS mutant colorectal cancer cells
In this study, we found that BLT2, a cell surface GPCR for leukotriene B4 and 12‑hydroxyeicosatetraenoic acid, is highly upregulated in KRAS mutant LOVO and SW480 colorectal cancer cells and plays critical roles in mediating proliferation through activation of phosphatidylinositol 3‑kinase (PI3K)/protein kinase B (Akt) and subsequent upregulation of cyclin D1. Exposure to BLT2 siRNA or LY255283, a specific BLT2 inhibitor, clearly suppressed the proliferation of KRAS mutant colorectal cancer cells and markedly increased cell cycle arrest by downregulating the PI3K/Akt-cyclin D1 cascade. Xenograft tumor formation by LOVO...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 14, 2018 Category: Molecular Biology Source Type: research

Resveratrol protects against isoproterenol induced myocardial infarction in rats through VEGF-B/AMPK/eNOS/NO signaling pathway.
In this study, we investigated the protective effect of RSV on myocardial infarction (MI) in rats and further explored the underlying signal pathway after VEGF-B. Rats received RSV or normal saline by intragastric administration for 7 consecutive days and followed by subcutaneously isoproterenol (ISO) or normal saline injections for another 2 days. We found that RSV pretreatment prevented the unfavorable changes in HW/BW, HW/TL, infarct size and cell apoptosis in ISO treated rats. Moreover, superoxide and malondialdehyde (MDA) production were significantly reduced and superoxide dismutase (SOD) was increased by RSV in ISO...
Source: Free Radical Research - December 13, 2018 Category: Research Tags: Free Radic Res Source Type: research

MicroRNA ‑223 promotes hepatocellular carcinoma cell resistance to sorafenib by targeting FBW7.
MicroRNA‑223 promotes hepatocellular carcinoma cell resistance to sorafenib by targeting FBW7. Oncol Rep. 2018 Dec 06;: Authors: Tang X, Yang W, Shu Z, Shen X, Zhang W, Cen C, Cao L, Zhang M, Zheng S, Yu J Abstract Hepatocellular carcinoma (HCC) is a globally prevalent malignancy associated with a poor patient prognosis. We investigated the relationship between microRNA‑223 (miR‑223) expression and the sensitivity of HCC cells to sorafenib treatment. miR‑223 expression was determined in HCC cell lines with differential sorafenib sensitivity using reverse transcription‑quantitative PCR. miR‑...
Source: Oncology Reports - December 13, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Glutathione peroxidase 4 maintains a stemness phenotype, oxidative homeostasis and regulates biological processes in Panc ‑1 cancer stem‑like cells.
Glutathione peroxidase 4 maintains a stemness phenotype, oxidative homeostasis and regulates biological processes in Panc‑1 cancer stem‑like cells. Oncol Rep. 2018 Dec 06;: Authors: Peng G, Tang Z, Xiang Y, Chen W Abstract Reactive oxygen species (ROS) have been widely accepted as critical molecules playing regulatory roles in various biological processes, including proliferation, differentiation and apoptotic/ferroptotic/necrotic cell death. Emerging evidence suggests that ROS may be involved in the induction of epithelial‑to‑mesenchymal transition (EMT), which has been reported to promote can...
Source: Oncology Reports - December 13, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Resveratrol Ameliorates Lipid Droplet Accumulation in Liver Through a SIRT1/ ATF6-Dependent Mechanism
Conclusion: Taken together, these findings indicate that RSV supplementation improves hepatic steatosis by ameliorating the accumulation of LDs, and this might be partially mediated by a SIRT1/ATF6-dependent mechanism.Cell Physiol Biochem 2018;51:2397 –2420
Source: Cellular Physiology and Biochemistry - December 13, 2018 Category: Cytology Source Type: research

Sex hormones alter the response of Toll-like receptor 3 to its specific ligand in fallopian tube epithelial cells.
Conclusion: These results imply that sex hormones exerted a suppressive effect on the function of TLR3 in the fallopian tube cell line when different concentrations of sex hormones were present. The current results also suggest that estrogen receptor beta and nuclear progesterone receptor B are likely to mediate the hormonal regulation of TLR3, as these two receptors are the main estrogen and progesterone receptors in OE-E6/E7 cell line. PMID: 30538945 [PubMed]
Source: Clinical and Experimental Reproductive Medicine - December 13, 2018 Category: Reproduction Medicine Tags: Clin Exp Reprod Med Source Type: research

Ambient fine particulate matter (PM2.5) induces oxidative stress and pro-inflammatory response via up-regulating the expression of CYP1A1/1B1 in human bronchial epithelial cells in vitro
Publication date: Available online 12 December 2018Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Qi Yuan, Yaoyao Chen, Xiaobo Li, Haiyan Chu, Zhengdong ZhangAbstractWe investigated the mechanism responsible for the oxidative stress and pro-inflammatory response triggered by PM2.5 collected from Nanjing of China. Two human bronchial epithelia cell lines (HBE and BEAS-2B) were used. Human gene expression profile microarray was performed to investigate the alteration of gene expression in PM2.5-treated HBE cells. The results of ROS assay and ELISA indicated that PM2.5 (150 µg/ml) incr...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - December 13, 2018 Category: Genetics & Stem Cells Source Type: research

TGF- β1 promotes proliferation and invasion of hepatocellular carcinoma cell line HepG2 by activating GLI-1 signaling.
CONCLUSIONS: TGF-β1 promotes HCC cell line HepG2 proliferation and invasion by activating GLI-1 signaling pathway. PMID: 30536311 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - December 13, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

DUSP1 promotes senescence of retinoblastoma cell line SO-Rb5 cells by activating AKT signaling pathway.
CONCLUSIONS: DUSP1 facilitated human retinoblastoma SO-Rb5 cell senescence induced by AGII through inhibiting Akt signaling pathway. PMID: 30536303 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - December 13, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

DUSP22 promotes senescence of HS-1 skin cancer cells through triggering MAPK signaling pathway.
CONCLUSIONS: DUSP22 facilitated HS-1 skin cancer cell aging via activating MAPK signal pathway, possibly providing novel strategy against skin cancer. PMID: 30536326 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - December 13, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Role and mechanism of Dvl3 in the esophageal squamous cell carcinoma.
CONCLUSIONS: Overall, down-regulation of Dvl3 expression can control the progression of esophageal squamous cell carcinoma, inhibit the growth and promote the apoptosis of tumor cells. Thus, Dvl3 has potential applications for early diagnosis, prognosis and therapeutics in the esophageal squamous cell carcinoma. PMID: 30536315 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - December 13, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

ASAP Tumor Microenvironment-Regulated Redox Responsive Cationic Galactose-Based Hyperbranched Polymers for siRNA Delivery
Bioconjugate ChemistryDOI: 10.1021/acs.bioconjchem.8b00785
Source: Bioconjugate Chemistry - December 13, 2018 Category: Biochemistry Authors: Yi-Yang Peng, Diana Diaz-Dussan, Piyush Kumar, Ravin Narain Source Type: research

GSE119038 Genome-wide binding of SUDHL4 DLBCL cells
Contributor : Katerina HatziSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensUsing ChIP-seq, we identified the genome-wide targets of BCL6 and LSD1 in lymphoma cells SUDHL4 and performed H3K4me1 ChIP-seq in cells treated with control siRNA or siRNA targeted to BCL6.
Source: GEO: Gene Expression Omnibus - December 13, 2018 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research

Molecules, Vol. 23, Pages 3306: 23-Hydroxyursolic Acid Isolated from the Stem Bark of Cussonia bancoensis Induces Apoptosis through Fas/Caspase-8-Dependent Pathway in HL-60 Human Promyelocytic Leukemia Cells
san Kyung-Tae Lee The natural product 23-hydroxyursolic acid (23-HUA) is a derivative of ursolic acid, which is known to induce cancer cell apoptosis. However, apoptotic effects and mechanisms of 23-HUA have not been well characterized yet. Herein, we investigated the molecular mechanisms of 23-HUA-induced apoptosis in HL-60 human promyelocytic leukemia cells. 23-HUA-treated HL-60 cells showed apoptotic features including internucleosomal DNA condensation and fragmentation as well as externalization of phosphatidylserine residues. 23-HUA induced a series of mitochondrial events including disruption of mitochondrial m...
Source: Molecules - December 13, 2018 Category: Chemistry Authors: Jong-Heon Won Kyung-Sook Chung Eun-Young Park Jeong-Hun Lee Jung-Hye Choi Leon Azefack Tapondjou Hee-Juhn Park Masaaki Nomura Ahmed H.E. Hassan Kyung-Tae Lee Tags: Article Source Type: research

Mineralocorticoid receptor deficiency improves the therapeutic effects of mesenchymal stem cells for myocardial infarction via enhanced cell survival.
Abstract The poor survival of stem cells seriously limits their therapeutic efficacy for myocardial infarction (MI). Mineralocorticoid receptor (MR) activation plays an important role in the pathogenesis of multiple cardiovascular diseases. Here, we examined whether MR silencing in bone marrow derived mesenchymal stem cells (MSCs) could improve MSCs' survival and enhance their cardioprotective effects in MI. MSCs from male Sprague-Dawley rats were transfected with adenoviral small interfering RNA to silence MR (siRNA-MR). MR silencing decreased hypoxia-induced MSCs' apoptosis, as demonstrated by Annexin V/7-AAD st...
Source: J Cell Mol Med - December 13, 2018 Category: Molecular Biology Authors: Xie X, Shen Y, Chen J, Huang Z, Ge J Tags: J Cell Mol Med Source Type: research

Chalcone flavokawain A attenuates TGF- β1-induced fibrotic pathology via inhibition of ROS/Smad3 signaling pathways and induction of Nrf2/ARE-mediated antioxidant genes in vascular smooth muscle cells.
In this study, flavokawain A (FKA, 2-30 μM), a naturally occurring chalcone in kava extracts, was evaluated for its anti-fibrotic and antioxidant properties in TGF-β1-stimulated vascular smooth muscle (A7r5) cells, as well as its underlying molecular mechanism of action. Immunofluorescence data showed down-regulated F-actin expression with FKA treatment in TGF-β1-stimulated A7r5 cells. Western blotting demonstrated that FKA treatment suppressed the expression of α-SMA and fibronectin proteins under TGF-β1 stimulation. Findings from wound-healing and invasion experiments showed that FKA inhibits TGF-β1-mediated migra...
Source: J Cell Mol Med - December 13, 2018 Category: Molecular Biology Authors: Hseu YC, Yang TY, Li ML, Rajendran P, Mathew DC, Tsai CH, Lin RW, Lee CC, Yang HL Tags: J Cell Mol Med Source Type: research

Heparan Sulfate Proteoglycan Synthesis is Dysregulated in Human Osteoarthritic Cartilage.
Abstract Osteoarthritis (OA) is a common degenerative joint disease, characterized by cartilage loss and subchondral bone remodelling in response to abnormal mechanical load. Heparan sulfate (HS) proteoglycans bind to many proteins that regulate cartilage homeostasis, including growth factors, morphogens, proteases, and their inhibitors, and modulate their localization, retention, and biological activity. Changes in HS expression and structure may thus have important consequences for joint health. We analyzed normal and osteoarthritic human knee cartilage, and found HS biosynthesis was markedly disrupted in OA, wi...
Source: The American Journal of Pathology - December 13, 2018 Category: Pathology Authors: Chanalaris A, Clarke H, Guimond SE, Vincent TL, Turnbull JE, Troeberg L Tags: Am J Pathol Source Type: research

Functional interaction between PDGF and GluN2B-containing NMDA receptors in smooth muscle cell proliferation and migration in pulmonary arterial hypertension.
In this study, we explored the complex interactions between platelet-derived growth factor (PDGF) and N-methyl D-aspartate receptor (NMDAR), and their effect on the excessive proliferation and migration of smooth muscle cells leading to obstructed arteries in Pulmonary Arterial Hypertension (PAH). We report lower expression of GluN2B, a subunit composing NMDARs expected to affect cell survival/proliferation of pulmonary artery smooth muscle cells (PASMCs), in PAH patients' lungs. PASMCs exposure to PDGF-BB, stimulated immediate increased levels of phosphorylated Src family kinases (SFKs) together with increased phosphoryla...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - December 13, 2018 Category: Cytology Authors: Quatredeniers M, Nakhleh MK, Dumas SJ, Courboulin A, Vinhas MC, Antigny F, Phan C, Guignabert C, Bendifallah I, Vocelle M, Fadel E, Dorfmüller P, Humbert M, Cohen-Kaminsky S Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

MMP-9-induced increase in intestinal tight junction permeability is mediated by P38 kinase signaling pathway activation of Myosin Light Chain Kinase gene.
Abstract Matrix Metalloproteinase-9 (MMP-9) has been implicated to be an important pathogenic factor in inflammatory bowel disease (IBD). MMP-9 is markedly elevated in intestinal tissue of patients with IBD, and IBD patients have a defective intestinal tight junction (TJ) barrier manifested by an increase in intestinal permeability. The loss of intestinal barrier function is a contributing factor in the development of intestinal inflammation, the role of MMP-9 in intestinal barrier function remains unclear. The purpose of this study was to investigate the effect of MMP-9 on intestinal epithelial TJ barrier and to ...
Source: American Journal of Physiology. Gastrointestinal and Liver Physiology - December 13, 2018 Category: Physiology Authors: Al-Sadi R, Youssef M, Rawat M, Guo S, Dokladny K, Haque M, Watterson DM, Ma TY Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research

High Mobility Group Box 1 (HMGB1) Predicts Invasion and Poor Prognosis of Glioblastoma Multiforme via Activating AKT Signaling in an Autocrine Pathway.
CONCLUSIONS HMGB1 is an independent prognostic biomarker for unfavorable prognosis of patients with GBM. Released HMGB1 of GBM cells can activate AKT and ERK signaling pathways and promote GBM cells invasion in this autocrine pathway, indicating that anti-HMGB1 therapy may be a promising treatment for GBM. PMID: 30531692 [PubMed - in process]
Source: Medical Science Monitor - December 12, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Sufficiency of hypoxia-inducible 2-oxoglutarate dioxygenases to block chemical oxidative stress-induced differentiation of human embryonic stem cells
Publication date: Available online 12 December 2018Source: Stem Cell ResearchAuthor(s): Eirini Koutsouraki, Steve Pells, Paul A. De SousaAbstractHypoxia benefits undifferentiated pluripotent stem cell renewal, and 2-oxoglutarate (2OG) dioxygenases have been implicated in pluripotent stem cell induction and renewal. We show in human embryonic stem cells (hESC) that an ambient oxygen-induced oxidative stress response elicited by culture in a hypoxic atmosphere (0.5% O2) correlates with the expression of 2OG dioxygenases, which oxidise DNA (TET1, 2, 3) and histone H3 (KDM4C), the former reflected by elevation in genomic 5-hyd...
Source: Stem Cell Research - December 12, 2018 Category: Stem Cells Source Type: research

IP3 Receptors Preferentially Associate with ER-Lysosome Contact Sites and Selectively Deliver Ca2+ to Lysosomes
Publication date: 11 December 2018Source: Cell Reports, Volume 25, Issue 11Author(s): Peace Atakpa, Nagendra Babu Thillaiappan, Stefania Mataragka, David L. Prole, Colin W. TaylorSummaryInositol 1,4,5-trisphosphate (IP3) receptors (IP3Rs) allow extracellular stimuli to redistribute Ca2+ from the ER to cytosol or other organelles. We show, using small interfering RNA (siRNA) and vacuolar H+-ATPase (V-ATPase) inhibitors, that lysosomes sequester Ca2+ released by all IP3R subtypes, but not Ca2+ entering cells through store-operated Ca2+ entry (SOCE). A low-affinity Ca2+ sensor targeted to lysosomal membranes reports large, l...
Source: Cell Reports - December 12, 2018 Category: Cytology Source Type: research

Muscovy duck reovirus p10.8 protein induces ER stress and apoptosis through the Bip/IRE1/XBP1 pathway
Publication date: Available online 12 December 2018Source: Veterinary MicrobiologyAuthor(s): Quanxi Wang, Mengxi Liu, Yuan Chen, Lihui Xu, Baocheng Wu, Yijan Wu, Yifan Huang, Wei-Ru Huang, Hung-Jen LiuAbstractIn the present study, the mechanisms underlying Muscovy duck reovirus (MDRV) p10.8 protein-induced ER stress and apoptosis in DF-1 cells and Muscovy duckling hepatic tissues were explored. On the fifth day post-infection, an increase in the mRNA levels of binding immunoglobulin protein (Bip) and X-box binding protein (XBP1), activation of XBP1/s, and an increase in percentage of apoptotic cells were observed in Muscov...
Source: Veterinary Microbiology - December 12, 2018 Category: Veterinary Research Source Type: research

Chrysin protects human osteoarthritis chondrocytes by inhibiting inflammatory mediator expression via HMGB1 suppression.
Authors: Zhang C, Yu W, Huang C, Ding Q, Liang C, Wang L, Hou Z, Zhang Z Abstract High‑mobility group box chromosomal protein (HMGB‑1) contributes to osteoarthritis (OA) by modulating various oxidative, inflammatory and apoptotic signaling pathways. The effect of chrysin (CH), a natural plant flavonoid, and its functional interaction with HMGB‑1, was investigated in a chondrocyte model of OA. Human chondrocytes were pre‑treated with CH, and then subsequently treated with IL‑1β to induce the formation of chondrocytes similar to those found in OA joints. Next, the expression level of HMGB‑1 was determine...
Source: Molecular Medicine Reports - December 12, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

No effect of sex on ethanol intake and preference after dopamine transporter (DAT) knockdown in adult mice
ConclusionsTaken together, these data provide further evidence that DAT plays an important role in controlling ethanol intake and that accumbal DAT contributes in the modulation of the reinforcing effects of ethanol. Overall, the results suggest that DAT inhibitors may be valuable in the pharmacotherapy of alcoholism.
Source: Psychopharmacology - December 12, 2018 Category: Psychiatry Source Type: research

Myo-inositol oxygenase accentuates renal tubular injury initiated by endoplasmic reticulum (er) stress.
MYO-INOSITOL OXYGENASE ACCENTUATES RENAL TUBULAR INJURY INITIATED BY ENDOPLASMIC RETICULUM (ER) STRESS. Am J Physiol Renal Physiol. 2018 Dec 12;: Authors: Tominaga T, Sharma I, Fujita Y, Doi T, Wallner AK, Kanwar YS Abstract Besides oxidant stress, ER stress has been implicated in the pathogenesis of various metabolic disorders affecting kidney. These two forms of stresses are not mutually exclusive to each other and may operate by a feedback loop in worsening the cellular injury. To attest to this contention studies were performed to assess if in such a setting there is worsening of tubulo-interstiti...
Source: American Journal of Physiology. Renal Physiology - December 12, 2018 Category: Physiology Authors: Tominaga T, Sharma I, Fujita Y, Doi T, Wallner AK, Kanwar YS Tags: Am J Physiol Renal Physiol Source Type: research

Polydatin Attenuates Atherosclerosis in ApoE - ∕- Mice through PBEF Mediated Reduction of Cholesterol Deposition.
In conclusion, Polydatin relieves atherosclerosis injury in ApoE -∕- mice, mainly through down-regulation of PBEF and inhibition of PBEF-inducing cholesterol deposits in macrophages. PMID: 30537866 [PubMed - as supplied by publisher]
Source: The American Journal of Chinese Medicine - December 11, 2018 Category: Complementary Medicine Authors: Huang Z, Tian G, Cheng S, Zhao D, Zhang Y, Jia Y, Zhou F Tags: Am J Chin Med Source Type: research

Cyclin ‐dependent kinase 9 promotes cervical cancer development via AKT2/p53 pathway
AbstractAberrant activation of cyclin ‐dependent kinase 9 (CDK9) is widespread in human cancers. However, the underlying mechanisms of CDK9 activation and the therapeutic potential of CDK9 inhibition in cervical cancer remain largely unknown. Here, we report that CDK9 is gradually upregulated during cervical lesion progression and reg ulated by HPV16 E6. CDK9 levels are highly correlated with FIGO stage, pathological grade, deep‐stromal invasion, tumor size, and lymph nodes metastasis. Knockdown of CDK9 by specific siRNA inhibits cervical cancer cell proliferation in vitro, as well as tumorigenesis in vivo. CDK9 inhibi...
Source: IUBMB Life - December 11, 2018 Category: Research Authors: Junfen Xu, Shanshan Xu, Yifeng Fang, Tingting Chen, Xing Xie, Weiguo Lu Tags: Research Communication Source Type: research

TRPC channels mediated calcium entry is required for proliferation of human airway smooth muscle cells induced by nicotine-nAChR.
In conclusion, nicotine-induced HASMC proliferation was mediated by TRPC3-dependent calcium entry via α5-nAchR, which provided a potential target for treatment of COPD. PMID: 30550855 [PubMed - as supplied by publisher]
Source: Biochimie - December 11, 2018 Category: Biochemistry Authors: Jiang Y, Zhou Y, Peng G, Tian H, Pan D, Liu L, Yang X, Li C, Li W, Chen L, Ran P, Dai A Tags: Biochimie Source Type: research

Down-regulation of lncRNA OGFRP1 induces autophagy and growth inhibition by AKT/mTOR signaling pathway in HCAECs.
In conclusion, we find that down-regulation of lncRNA induces autophagy and inhibited the proliferation, migration and invasion by AKT/mTOR signaling pathway in HCAECs. PMID: 30536541 [PubMed - as supplied by publisher]
Source: Cell Biology International - December 11, 2018 Category: Cytology Authors: Zhang X, Liu J, Gu Y, Sun C, Qu F Tags: Cell Biol Int Source Type: research

Cyclin ‐dependent kinase 9 promotes cervical cancer development via AKT2/p53 pathway
AbstractAberrant activation of cyclin ‐dependent kinase 9 (CDK9) is widespread in human cancers. However, the underlying mechanisms of CDK9 activation and the therapeutic potential of CDK9 inhibition in cervical cancer remain largely unknown. Here, we report that CDK9 is gradually upregulated during cervical lesion progression and reg ulated by HPV16 E6. CDK9 levels are highly correlated with FIGO stage, pathological grade, deep‐stromal invasion, tumor size, and lymph nodes metastasis. Knockdown of CDK9 by specific siRNA inhibits cervical cancer cell proliferation in vitro, as well as tumorigenesis in vivo. CDK9 inhibi...
Source: IUBMB Life - December 11, 2018 Category: Research Authors: Junfen Xu, Shanshan Xu, Yifeng Fang, Tingting Chen, Xing Xie, Weiguo Lu Tags: Research Communication Source Type: research

Markers of Accelerated Skeletal Muscle Regenerative Response in MRL/MpJ Mice: Characteristics of Muscle Progenitor Cells and Circulating Factors
This study suggests that a combination of MPCs and modulating antio xidative stress can represent a novel therapy to improve muscle healing after injury, disease, and aging.© AlphaMed Press 2018
Source: Stem Cells - December 10, 2018 Category: Stem Cells Authors: Chieh Tseng, Krishna Sinha, Haiying Pan, Yan Cui, Ping Guo, Chih Yi Lin, Fan Yang, Zhenhan Deng, Holger K. Eltzschig, Aiping Lu, Johnny Huard Tags: Regenerative Medicine Source Type: research

Di-2-ethylhexylphthalate promotes thyroid cell proliferation and DNA damage through activating thyrotropin-receptor-mediated pathways in vitro and in vivo.
Abstract Phthalates are being suggested to be associated with altered thyroid function and proliferative changes, but detailed mechanisms remain unclear. Here, we examined the effects of di-(2-ethylhexyl) phthalate (DEHP) on DNA damage and proliferation in thyroid using thyroid carcinoma cell line, 8505C, in vitro and the rats orally treated with DEHP at 0, 0.3, 3, 30 and 150 mg/kg for 90 days from post-natal day 9 in vivo. Exposure to DHEP (1-50 μM) induced cellular proliferation, as evidenced by increased cell viability and DNA synthesis. Activation of γH2AX, a sensitive biomarker for DNA damage was observ...
Source: Food and Chemical Toxicology - December 10, 2018 Category: Food Science Authors: Kim S, Park GY, Yoo YJ, Jeong JS, Nam KT, Jee SH, Lim KM, Lee YS Tags: Food Chem Toxicol Source Type: research

Hepatitis C Virus Entry into Macrophages/Monocytes Mainly Depends on the Phagocytosis of Macrophages
ConclusionsHCV entry into macrophages mainly depends on phagocytosis of macrophages.
Source: Digestive Diseases and Sciences - December 10, 2018 Category: Gastroenterology Source Type: research

Inhibition of HOXB7 suppresses p27-mediated acute lymphoblastic leukemia by regulating basic fibroblast growth factor and ERK1/2
In this study, we explored the molecular mechanism of HOXB7 in cell viability and cell cycle in ALL cell lines.Materials and methodsPeripheral blood lymphocytes was isolated by Isopycnic Ficoll-Hypaque solution; Relative mRNA expression of HOXB7 was measured by RT-qPCR; Relative protein expressions of HOXB7, p27, bFGF, pERK1/2 were tested by Western blot assay; Cell viability was tested by MTT; Cell proliferation was detected by BrdU assay; 2.8. Cell cycle was analyzed by flow cytometry.Key findingsHOXB7 was significantly elevated in peripheral blood lymphocytes of patients with ALL. HOXB7 was inhibited by HOXB7 siRNA tran...
Source: Life Sciences - December 9, 2018 Category: Biology Source Type: research

1,25 ‑Dihydroxyvitamin D regulates macrophage polarization and ameliorates experimental inflammatory bowel disease by suppressing miR-125b.
This study aimed to explore whether 1,25(OH)2D3 modulates macrophage polarization in inflammation. Peripheral blood mononuclear cells and colitis mice were treated with 1,25(OH)2D3. Macrophages were transfected with siRNA-vitamin D receptor (VDR) or miR-125b mimic or inhibitor, and 1,25(OH)2D3-pretreated colitis mice were injected with a miR-125b agomir. The distribution of macrophage subsets and macrophage subtype characteristics was analyzed. As expected, 1,25(OH)2D3 transformed lipopolysaccharide-induced M1 macrophages to the M2 subset, downregulated tumor necrosis factor-α and interleukin (IL)-6 expression and interfe...
Source: International Immunopharmacology - December 9, 2018 Category: Allergy & Immunology Authors: Zhu X, Zhu Y, Li C, Yu J, Ren D, Qiu S, Nie Y, Yu X, Xu X, Zhu W Tags: Int Immunopharmacol Source Type: research

Tropomyosin Tpm 2.1 loss induces glioblastoma spreading in soft brain-like environments
ConclusionsWe propose that Tpm 2.1 down-regulation facilitates GBM colonisation of the soft brain environment. This specialisation of the GBM actin cytoskeleton organisation that is highly suited to the soft brain-like environment may provide novel therapeutic targets for arresting GBM invasion.
Source: Journal of Neuro-Oncology - December 9, 2018 Category: Cancer & Oncology Source Type: research