Query: siRNA

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Total 18551 results found since Jan 2013.

Inhibition of CSF1R and AKT by ( ±)-kusunokinin hinders breast cancer cell proliferation.
Inhibition of CSF1R and AKT by (±)-kusunokinin hinders breast cancer cell proliferation. Biomed Pharmacother. 2020 Jun 11;129:110361 Authors: Rattanaburee T, Tipmanee V, Tedasen A, Thongpanchang T, Graidist P Abstract Kusunokinin, a lignan compound, inhibits cancer cell proliferation and induces apoptosis; however, the role of kusunokinin is not fully understood. Here, we aimed to identify a target protein of (-)-kusunokinin and determine the protein levels of its downstream molecules. We found that (-)-kusunokinin bound 5 possible target proteins, including CSF1R, MMP-12, HSP90-α, CyclinB1 and MEK1...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - June 11, 2020 Category: Drugs & Pharmacology Authors: Rattanaburee T, Tipmanee V, Tedasen A, Thongpanchang T, Graidist P Tags: Biomed Pharmacother Source Type: research

Hypoxia induced up-regulation of tissue factor is mediated through extracellular RNA activated Toll-like receptor 3-activated protein 1 signalling.
Abstract Sterile Inflammation (SI), a condition where damage associated molecular patterns (DAMPs) released from dying cells, leads to TLR (Toll-like receptor) activation and triggers hypoxemia in circulation leading to venous thrombosis (VT) through tissue factor (TF) activation, but its importance under acute hypoxia (AH) remains unexplored. Thus, we hypothesized that eRNA released from dying cells under AH activates TF via the TLR3-ERK1/2-AP1 pathway, leading to VT. Animals were exposed to stimulate hypoxia for 0-24 h at standard temperature and humidity. RNaseA and DNase1 were injected immediately before expo...
Source: Blood Cells, Molecules and Diseases - June 11, 2020 Category: Hematology Authors: Bhagat S, Biswas I, Ahmed R, Khan GA Tags: Blood Cells Mol Dis Source Type: research

Anti-inflammatory effects of higenamine (Hig) on LPS-activated mouse microglia (BV2) through NF- κB and Nrf2/HO-1 signaling pathways.
Anti-inflammatory effects of higenamine (Hig) on LPS-activated mouse microglia (BV2) through NF-κB and Nrf2/HO-1 signaling pathways. Int Immunopharmacol. 2020 Jun 11;85:106629 Authors: Yang S, Chu S, Ai Q, Zhang Z, Gao Y, Lin M, Liu Y, Hu Y, Li X, Peng Y, Pan Y, He Q, Chen N Abstract Microglia are the most widely equipped protective cells in the brain and play a pivotal role in the development of neurological diseases. Inflammatory response and oxidative stress are critical risk factors in the activation of microglia which may cause various neurological diseases. Higenamine (Hig), a plant-based alkal...
Source: International Immunopharmacology - June 11, 2020 Category: Allergy & Immunology Authors: Yang S, Chu S, Ai Q, Zhang Z, Gao Y, Lin M, Liu Y, Hu Y, Li X, Peng Y, Pan Y, He Q, Chen N Tags: Int Immunopharmacol Source Type: research

Ribonuclease H2 Subunit A impacts invasiveness and chemoresistance resulting in poor survivability of breast cancer in ER dependent manner.
Authors: Shen J, Lin S, Liu L, Wang C Abstract Ribonuclease H2 subunit A (RNASEH2A), a member of the RNase HII family, acts in DNA replication by mediating removal of lagging-strand Okazaki fragment RNA primers. We explored the roles of RNASEH2A in the prognosis of breast cancer, specifically in relation to proliferation, invasiveness, and sensitivity to cytotoxins of cells in the estrogen receptor (ER)-positive MCF-7 and the ER-negative MDA-MB-231 breast cancer cell lines. We collected 26 datasets from around the world, comprising 7815 accessible cases. In these datasets, we probed the association between expressi...
Source: American Journal of Translational Research - June 10, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Effects of the multifunctional hormone leptin on orthodontic tooth movement in rats.
This study aims to investigate the effects of leptin, which is a multifunctional hormone, on orthodontic tooth movement (OTM) and the underlying mechanism. Sprague-Dawley rat OTM models were established and divided into two groups with the administration of vehicle or leptin respectively. Stereomicroscope and microcomputed tomography were used to evaluate the amount of OTM. TRAP staining, immunohistochemical and immunofluorescence staining were used to detect osteoclasts and relative protein expressions. After treated with compression force, human periodontal ligament cells (hPDLCs) were co-cultured with human peripheral b...
Source: American Journal of Translational Research - June 10, 2020 Category: Research Tags: Am J Transl Res Source Type: research

SIRT4 suppresses the inflammatory response and oxidative stress in osteoarthritis.
This study aimed to explore the role of SIRT4 during OA and mechanisms implicated. We extracted total protein and mRNA of the cartilage from OA patients and isolated the chondrocytes from the cartilage in different degenerated degrees for cell culture. Collagen II and SIRT4 levels of the tissues were analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) and Western blot. Chondrocytes were transferred with SIRT4-siRNA, treated with recombinant human SIRT4 protein for 24 h, respectively. Aggrecan, collagen I, collagen II, MMP-13, IL-6, TNF-α, SOD1, SOD2, and CAT expression, and ROS levels were in...
Source: American Journal of Translational Research - June 10, 2020 Category: Research Tags: Am J Transl Res Source Type: research

New mechanism of nerve injury in Alzheimer's disease: β-amyloid-induced neuronal pyroptosis.
In conclusion, Aβ1-42 could induce pyroptosis by GSDMD protein, and NLRP3-caspase-1 signalling was an important signal to mediate GSDMD cleavage, which plays an important role in Aβ1-42 -induced pyroptosis in neurons. Therefore, GSDMD is expected to be a novel therapeutic target for AD. PMID: 32521573 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - June 10, 2020 Category: Molecular Biology Authors: Han C, Yang Y, Guan Q, Zhang X, Shen H, Sheng Y, Wang J, Zhou X, Li W, Guo L, Jiao Q Tags: J Cell Mol Med Source Type: research

The Use of RNAi Technology to Interfere with Zfx Gene Increases the Male Rates of Red Deer (Cervus elaphus) Offspring.
This study further confirms that the Zfx gene plays a significant role in the process of X spermatogenesis. PMID: 32509876 [PubMed - in process]
Source: Biomed Res - June 9, 2020 Category: Research Authors: Wei L, Xi J, Zhang Y, Zeng B, Li F, Wang K, Zhang S, Zhao X, Li Y, Shen H, Jiang S, Jia B Tags: Biomed Res Int Source Type: research

Myocyte-specific enhancer factor 2D promotes tumorigenesis and progression in tongue squamous cell carcinoma.
Abstract Tongue squamous cell carcinoma (TSCC) ranks as one of the most common cancers worldwide and has a poor prognosis. Myocyte-specific enhancer factor 2 (MEF2D) has recently been considered as a novel factor involved in cancer development. In the present study, the function and underlying mechanism of MEF2D in TSCC were investigated. The levels of MEF2D mRNA and protein were determined in human TSCC samples by RT-qPCR and western blot, respectively. The interaction between MEF2D expression and clinicopathologic features was evaluated by IHC and analysis of clinical information. MEF2D-mediated effects on proli...
Source: International Journal of Clinical and Experimental Pathology - June 9, 2020 Category: Pathology Authors: Liu Y, Yu W, Zhu Y, Sun Z, Huang X, Zhou J, Yao R, Zhang Q, Qiu J, Yue L Tags: Int J Clin Exp Pathol Source Type: research

Galectin-3 inhibition attenuates doxorubicin-induced cardiac dysfunction by upregulating the expression of peroxiredoxin-4.
In conclusion, Gal-3 mediated Dox-induced cardiotoxicity and Gal-3 inhibition attenuated Dox-induced cardiac dysfunction by upregulating the expression of Prx-4 to reduce myocardial oxidative stress. PMID: 32516552 [PubMed - as supplied by publisher]
Source: Canadian Journal of Physiology and Pharmacology - June 9, 2020 Category: Drugs & Pharmacology Authors: Tian Y, Lv W, Lu C, Jiang Y, Yang X, Song M Tags: Can J Physiol Pharmacol Source Type: research

GSE140498 Hepatocytic c-Jun N-terminal kinases (JNK)-1/2 function determines cell fate during carcinogenesis
Contributors : F J Cubero ; M R Mohamed ; M V Boekschoten ; C TrautweinSeries Type : Expression profiling by arrayOrganism : Mus musculusAberrant biliary hyperproliferation resulting from lack of differentiating signals favoring the maintenance of an immature and proliferative phenotype by biliary epithelial cells are ultimately responsible for ducto/cystogenesis and intrahepatic cholangiocarcinoma (CCA) formation. Mitogen-activated protein kinase (MAPK) signaling is pivotal for CCA-related tumorigenesis. In particular, targeted inhibition of JNK signaling has shown therapeutic potential. However, the cell-type specific ro...
Source: GEO: Gene Expression Omnibus - June 8, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research

GSE127956 Wisp inhibits chondrogenic differentiation in heterotopic ossification
Contributors : Aaron James ; Ching Y Hsu ; Carolyn Meyers ; Jiajia XuSeries Type : Expression profiling by arrayOrganism : Mus musculusHeterotopic ossification (HO) is the formation of extra-skeletal bone in muscle and soft tissues. Better characterization of the signaling pathways predisposing to HO is critical to identifying therapies directed against this common and potentially debilitating condition. WISP-1 (WNT1-inducible-signaling pathway protein 1) is a CCN family member and is expressed in skeletal cells during bone development or repair. Here, we sought to comprehensively explore the significance of WISP-1 across ...
Source: GEO: Gene Expression Omnibus - June 8, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research

Piezo1 activates the NLRP3 inflammasome in nucleus pulposus cell-mediated by Ca2+/NF- κB pathway.
Piezo1 activates the NLRP3 inflammasome in nucleus pulposus cell-mediated by Ca2+/NF-κB pathway. Int Immunopharmacol. 2020 Jun 08;85:106681 Authors: Sun Y, Leng P, Song M, Li D, Guo P, Xu X, Gao H, Li Z, Li C, Zhang H Abstract Studying and understanding the mechanism of inflammation in nucleus pulposus is the key to understand and prevent intervertebral disc degeneration. We propose a model of mechanical sensitive ion channel Piezo1 mediated inflammation of nucleus pulposus cells. Piezo1 can up-regulate the level of interleukin-1β (IL-1β) in nucleus pulposus cells once it is activated. It is sugges...
Source: International Immunopharmacology - June 8, 2020 Category: Allergy & Immunology Authors: Sun Y, Leng P, Song M, Li D, Guo P, Xu X, Gao H, Li Z, Li C, Zhang H Tags: Int Immunopharmacol Source Type: research

The membrane protein sortilin can be targeted to inhibit pancreatic cancer cell invasion.
In this study, we found that sortilin expression was higher in pancreatic cell lines vs normal pancreatic ductal epithelial cells, as demonstrated by Western blot and mass spectrometry. The increased sortilin level in pancreatic cancer cells was confirmed by immunohistochemistry in a series of 99 human pancreatic adenocarcinomas vs 48 normal pancreatic tissues (p=0.0014). Sortilin inhibition by siRNA and the pharmacological inhibitor AF38469 strongly reduced the adhesion and invasion of pancreatic cancer cells without affecting cell survival and viability. Sortilin inhibition also decreased the phosphorylation of the focal...
Source: The American Journal of Pathology - June 8, 2020 Category: Pathology Authors: Gao F, Griffin N, Faulkner S, Li X, King SJ, Jobling P, Denham JW, Jiang CC, Hondermarck H Tags: Am J Pathol Source Type: research

Glycyrrhizin improved autophagy flux via HMGB1-dependent Akt/mTOR signaling pathway to prevent Doxorubicin-induced cardiotoxicity.
Abstract Doxorubicin (DOX) is one of the most effective and irreplaceable chemotherapeutic agents but its clinical use is limited due to its cardiotoxicity. Glycyrrhizin(GL) has been applied to liver disorders for long. However, little is known that if GL could be meaningful in attenuating cardiotoxicity. The aim of this study is to investigate the cardioprotective effects of GL in DOX-induced cardiotoxicity (DIC) and the underlying mechanism. Here, H9c2 cardiomyoblasts, Neonatal rat cardiomyocytes (NRCMs), and Rats were introduced as test models. A single dose of 20 mg/kg DOX (i.p.) was applied to induce acute ...
Source: Toxicology - June 7, 2020 Category: Toxicology Authors: Lv X, Zhu Y, Deng Y, Zhang S, Zhang Q, Zhao B, Li G Tags: Toxicology Source Type: research

Estrogen receptors participate in silibinin-caused nuclear translocation of apoptosis-inducing factor in human breast cancer MCF-7 cells.
Abstract Our previous studies showed that silibinin promoted activation of caspases to induce apoptosis in human breast cancer MCF-7 cells by down-regulating the protein expression level of estrogen receptor (ER) α and up-regulating ERβ. Recently, it has been reported that silibinin-induced apoptosis also involved nuclear translocation of apoptosis-inducing factor (AIF). Here we report that silibinin induces nuclear translocation of AIF through the down-regulation of ERα and up-regulation of ERβ in a concentration dependent manner in MCF-7 cells. AIF knockdown with siRNA significantly reverses silibinin-in...
Source: Archives of Biochemistry and Biophysics - June 7, 2020 Category: Biochemistry Authors: Liu W, Ji Y, Sun Y, Si L, Fu J, Hayashi T, Onodera S, Ikejima T Tags: Arch Biochem Biophys Source Type: research

Resveratrol inhibits proliferation and promotes apoptosis of keloid fibroblasts by targeting HIF-1 α.
In this study, keloid-derived fibroblasts were cultured under hypoxia environment and was treated by resveratrol. CCK-8 assay and Annexin V-FITC were used to evaluate cell activity and apoptosis level. Western blot and RT-qPCR were also used to assess the expression of HIF-α, Collagen I and Collagen III. Besides, siRNA was also used to explore the mechanisms of resveratrol's effect. In this study, hypoxia promotes proliferation and inhibits apoptosis of keloid fibroblasts. These findings highlight the potential obstacle in treating keloids. Furthermore, we demonstrated that resveratrol could reverse the effect of hypoxia ...
Source: Journal of Plastic Surgery and Hand Surgery - June 6, 2020 Category: Surgery Tags: J Plast Surg Hand Surg Source Type: research

ILK silencing inhibits migration and invasion of more invasive glioblastoma cells by downregulating ROCK1 and Fascin-1.
In this study, we used two brain cell lines, the non-invasive neuroglioma H4 cells, and the highly invasive glioblastoma A172 cells, which express ILK in much higher levels than H4. We studied the effect of ILK silencing on the metastatic behavior of glioblastoma cells in vitro and elucidate the underlying molecular mechanism. We showed that siRNA-mediated silencing of ILK inhibits cell migration and invasion of the highly invasive A172 cells while it does not affect the migratory and invasive capacity of H4 cells. These data were also supported by respective changes in the expression of Rho-associated kinase 1 (ROCK1), fa...
Source: Molecular and Cellular Biochemistry - June 6, 2020 Category: Biochemistry Authors: Louca M, Zaravinos A, Stylianopoulos T, Gkretsi V Tags: Mol Cell Biochem Source Type: research

TIMP1 down-regulation enhances gemcitabine sensitivity and reverses chemoresistance in pancreatic cancer.
Abstract The therapeutic effect of gemcitabine (GEM) in pancreatic ductal adenocarcinoma (PDAC) is limited due to low drug sensitivity and high drug resistance. Tissue inhibitor of matrix metalloprotease 1 (TIMP1) is reportedly associated with GEM resistance in PDAC. However, the effect of TIMP1 down-regulation in combination with GEM treatment is unknown. We analyzed the expression of TIMP1 in human PDAC tissue using western blot, quantitative real-time polymerase chain reaction (qRT-PCR), and immunohistochemistry. TIMP1 was highly expressed in PDAC specimens. Kaplan-Meier survival analysis suggested that a highe...
Source: Biochemical Pharmacology - June 6, 2020 Category: Drugs & Pharmacology Authors: Tan Y, Li X, Tian Z, Chen S, Zou J, Lian G, Chen S, Huang K, Chen Y Tags: Biochem Pharmacol Source Type: research

Roles of circ-CSPP1 on the proliferation and metastasis of glioma cancer.
CONCLUSIONS: Circ-CSPP1 promoted the proliferation, invasion, and migration of GC cells. PMID: 32495924 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 5, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

MiR-449b-5p inhibits human glioblastoma cell proliferation by inactivating WNT2B/Wnt/ β-catenin signaling pathway.
CONCLUSIONS: MiR-449b-5p acts as a tumor suppressor and retards the oncogenesis of glioblastoma, which is achieved via inactivation of Wnt/β-catenin signaling by directly targeting WNT2B. PMID: 32495889 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 5, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Expression of lncRNA AK058003 in esophageal carcinoma and analysis of its intervention effect.
CONCLUSIONS: LncRNA AK058003 is highly expressed in EC patients, which promotes proliferation, migration, invasion, and metastasis of EC cells. In addition, the postoperative prognosis of EC patients with high expression of lncRNA AK058003 is relatively poor. PMID: 32495875 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 5, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

LINC00473 functions as an oncogene and predicts poor prognosis in pancreatic cancer via the cAMP/ β-catenin axis.
CONCLUSIONS: LINC00473 was upregulated in PC tissues and cells, indicating a poor prognosis and clinical pathological features of PC. It promoted PC progression via activating the cAMP/β-catenin axis, which provided a novel target for the prediction for PC diagnosis, biological therapy, and prognosis. PMID: 32495868 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 5, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Matrilin-3 alleviates extracellular matrix degradation of nucleus pulposus cells via induction of IL-1 receptor antagonist.
CONCLUSIONS: This study provides a novel viewpoint of Matrilin-3 in the ECM stability of NP due to its ability to activate IL-1Ra. It is considered that MATN3 efficiently protects ECM degeneration of human NP cells related to maintain the content of Collagen II and aggrecan, as well as inflammatory inhibition. PMID: 32495856 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 5, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

MTIM affects retinal ganglion cells through PI3K/AKT signaling pathway.
CONCLUSIONS: Up-regulation of MT1M can inhibit RGC cell apoptosis and inflammation, and promote RGC cell proliferation through the PI3K/AKT signaling pathway. PMID: 32495853 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 5, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

GSE135085 Whole transcriptomes analyses of gastric cancer HGC-27 cell line treated with siSTRN3 mixture or negative control siRNA
Contributors : Yang Tang ; Shi JiaoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPurpose: To explore the molecular mechanisms of STRN3 regulating gastic cancer tumorigenesis, RNA sequencing was performed to analyze the geome-wide change of siSTRN3 treated HGC-27 cells compared to those of control cells.Methods: Total mRNA was extracted from HGC-27 cells in triplicate respectively. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina Hiseq 4000 platform. As a reasult, we got the transcript data using Hisat2 followed by Str...
Source: GEO: Gene Expression Omnibus - June 5, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

miR-623 suppresses cell proliferation, migration and invasion through direct inhibition of XRCC5 in breast cancer.
CONCLUSION: miR-623 suppressed cell proliferation, migration and invasion through downregulation of cyclin dependent kinases and inhibition of the phosphatidylinositol-3-kinase (PI3K)/Akt and Wnt/β-Catenin pathways by targeting XRCC5. METHODS: miR-623 was either overexpressed in breast cancer cell lines through exogenous transfection or knocked down by specific siRNA. Cell proliferation, migration and invasion were examined using CCK-8, colony formation and transwell assay. The direct target of miR-623 was verified using luciferase reporter gene assay. PMID: 32501811 [PubMed - as supplied by publisher]
Source: Aging - June 5, 2020 Category: Biomedical Science Authors: Li Q, Liu J, Jia Y, Li T, Zhang M Tags: Aging (Albany NY) Source Type: research

Chronic treatment with the complex I inhibitor MPP+ depletes endogenous PTEN-induced kinase 1 (PINK1) via up-regulation of Bcl-2-associated athanogene 6 (BAG6) Neurobiology
Mitochondrial dysfunction is implicated in sporadic and familial Parkinson's disease (PD). However, the mechanisms that impair homeostatic responses to mitochondrial dysfunction remain unclear. Previously, we found that chronic, low-dose administration of the mitochondrial complex I inhibitor 1-methyl-4-phenylpyridinium (MPP+) dysregulates mitochondrial fission–fusion, mitophagy, and mitochondrial biogenesis. Given that PTEN-induced kinase 1 (PINK1) regulates mitochondrial function, dynamics, and turnover, we hypothesized that alterations in endogenous PINK1 levels contribute to depletion of mitochondria during chronic c...
Source: Journal of Biological Chemistry - June 5, 2020 Category: Chemistry Authors: Manish Verma, Jianhui Zhu, Kent Z. Q. Wang, Charleen T. Chu Tags: Molecular Bases of Disease Source Type: research

Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle Cell Biology
Chikungunya fever is a re-emerging zoonotic disease caused by chikungunya virus (CHIKV), a member of the Alphavirus genus in the Togaviridae family. Only a few studies have reported on the host factors required for intracellular CHIKV trafficking. Here, we conducted an imaging-based siRNA screen to identify human host factors for intracellular trafficking that are involved in CHIKV infection, examined their interactions with CHIKV proteins, and investigated the contributions of these proteins to CHIKV infection. The results of the siRNA screen revealed that host endosomal sorting complexes required for transport (ESCRT) pr...
Source: Journal of Biological Chemistry - June 5, 2020 Category: Chemistry Authors: Shiho Torii, Yasuko Orba, Michihito Sasaki, Koshiro Tabata, Yuji Wada, Michael Carr, Jody Hobson-Peters, Roy A. Hall, Ayato Takada, Takasuke Fukuhara, Yoshiharu Matsuura, William W. Hall, Hirofumi Sawa Tags: Microbiology Source Type: research

Chrysophanol ameliorates renal interstitial fibrosis by inhibiting the TGF- β/Smad signaling pathway.
Chrysophanol ameliorates renal interstitial fibrosis by inhibiting the TGF-β/Smad signaling pathway. Biochem Pharmacol. 2020 Jun 05;:114079 Authors: Dou F, Ding Y, Wang C, Duan J, Wang W, Xu H, Zhao X, Wang J, Wen A Abstract Renal interstitial fibrosis (RIF) is a major pathological feature of chronic kidney disease at middle and end stages. Chrysophanol (CP), 1,8-dihydroxy-3-methyl-9,10-anthraquinone, is an anthraquinone isolated from Rheum palmatum L.with a variety of pharmacological activities including the suppression of RIF. However, the effect of CP on renal fibrosis and its potential mechanism ...
Source: Biochemical Pharmacology - June 5, 2020 Category: Drugs & Pharmacology Authors: Dou F, Ding Y, Wang C, Duan J, Wang W, Xu H, Zhao X, Wang J, Wen A Tags: Biochem Pharmacol Source Type: research

Inhibition of long noncoding RNA MALAT1 suppresses high glucose-induced apoptosis and inflammation in human umbilical vein endothelial cells by suppressing the NF- κB signaling pathway.
In conclusion, our study demonstrated that MALAT1 was upregulated in HG-induced HUVECs, and inhibition of MALAT1 could inhibit HG-induced apoptosis and inflammation in HUVECs by suppression of the NF-κB signaling pathway. PMID: 32502356 [PubMed - as supplied by publisher]
Source: Biochemistry and Cell Biology - June 5, 2020 Category: Biochemistry Authors: Gong YP, Zhang YW, Su XQ, Gao HB Tags: Biochem Cell Biol Source Type: research

Nanoparticle-based co-delivery of siRNA and paclitaxel for dual-targeting of glioblastoma
Nanomedicine, Ahead of Print.
Source: Future Medicine: Nanomedicine - June 4, 2020 Category: Nanotechnology Authors: Jun Yang Qiang Zhang Yanxiu Liu Xinjie Zhang Wenjun Shan Shefang Ye Xi Zhou Yunlong Ge Xiumin Wang Lei Ren Source Type: research

miR-137 prevents inflammatory response, oxidative stress, neuronal injury and cognitive impairment via blockade of Src-mediated MAPK signaling pathway in ischemic stroke.
In conclusion, our work suggests that, in mice, miR-137 confers neuroprotective effects against ischemic stroke via attenuation of oxidative, apoptotic, and inflammatory pathways through inhibiting Src-dependent MAPK signaling pathway. PMID: 32496209 [PubMed - as supplied by publisher]
Source: Aging - June 4, 2020 Category: Biomedical Science Authors: Tian R, Wu B, Fu C, Guo K Tags: Aging (Albany NY) Source Type: research

Cancers, Vol. 12, Pages 1462: SPOP Deregulation Improves the Radiation Response of Prostate Cancer Models by Impairing DNA Damage Repair
This study aimed to define the role of SPOP deregulation (by gene mutation or knockdown) as a radiosensitizing factor in PCa preclinical models. To express WT or mutant (Y87N, K129E and F133V) SPOP, DU145 and PC-3 cells were transfected with pMCV6 vectors. Sensitivity profiles were assessed using clonogenic assay and immunofluorescent staining of γH2AX and RAD51 foci. SCID xenografts were treated with 5 Gy single dose irradiation using an image-guided small animal irradiator. siRNA and miRNA mimics were used to silence SPOP or express the SPOP negative regulator miR-145, respectively. SPOP deregulation, by ei...
Source: Cancers - June 4, 2020 Category: Cancer & Oncology Authors: Rihan El Bezawy Martina Tripari Stefano Percio Alessandro Cicchetti Monica Tortoreto Claudio Stucchi Stella Tinelli Valentina Zuco Valentina Doldi Paolo Gandellini Riccardo Valdagni Nadia Zaffaroni Tags: Article Source Type: research

Induction of SPARC on Oxidative Stress, Inflammatory Phenotype Transformation, and Apoptosis of Human Brain Smooth Muscle Cells Via TGF- β1-NOX4 Pathway
AbstractSecreted protein acidic and rich in cysteine (SPARC) has a close association with inflammatory response and oxidative stress in tissues and is widely expressed in intracranial aneurysms (IAs), especially in smooth muscle cells. Therefore, it is inferred that SPARC might be involved in the formation and development of IAs through the inflammatory response pathway or oxidative stress pathway. The aim of this study is to investigate the pathological mechanism of SPARC in oxidative stress, inflammation, and apoptosis during the formation of IAs, as well as the involvement of TGF- β1 and NOX4 molecules. Human brain vas...
Source: Journal of Molecular Neuroscience - June 3, 2020 Category: Neuroscience Source Type: research

CREB activity is required for mTORC1 signaling-induced primordial follicle activation in mice
AbstractIn mammals, progressive activation of primordial follicles is essential for maintenance of the reproductive lifespan. Several reports have demonstrated that mitogen-activated protein kinases 3 and 1 (MAPK3/1) –mammalian target of rapamycin complex 1 (mTORC1) signaling in pre-granulosa cells promotes primordial follicle activation by increasing KIT ligand (KITL) expression and then stimulating phosphatidylinositol 3 kinase signaling in oocytes. However, the mechanism of mTORC1 signaling in the promotion of KITL expression is unclear. Immunofluorescence staining results showed that phosphorylated cyclic AMP respons...
Source: Histochemistry and Cell Biology - June 3, 2020 Category: Biomedical Science Source Type: research

Roles for Drosophila cap1 2'-O-ribose methyltransferase in the small RNA silencing pathway associated with Argonaute 2.
Abstract Cap1 2'-O-ribose methyltransferase (CMTR1) modifies RNA transcripts containing the 7-methylguanosine cap via 2'-O-ribose methylation of the first transcribed nucleotide, yielding cap1 structures. However, the role of CMTR1 in small RNA-mediated gene silencing remains unknown. Here, we identified and characterized a Drosophila CMTR1 gene (dCMTR1) mutation. We found that the catalytic activity of dCMTR1 was involved in the biogenesis of small interfering RNAs (siRNAs) but not microRNAs. Additionally, dCMTR1 interacted with R2D2, a key component for the assembly of the RNA-induced silencing complex (RISC) co...
Source: Insect Biochemistry and Molecular Biology - June 3, 2020 Category: Biochemistry Authors: Lee S, Hong JS, Lim DH, Lee YS Tags: Insect Biochem Mol Biol Source Type: research

ASAP Fluorescent Peptide Dendrimers for siRNA Transfection: Tracking pH Responsive Aggregation, siRNA Binding, and Cell Penetration
Bioconjugate ChemistryDOI: 10.1021/acs.bioconjchem.0c00231
Source: Bioconjugate Chemistry - June 2, 2020 Category: Biochemistry Authors: Marc Heitz, Susanna Zamolo, Sacha Javor, and Jean-Louis Reymond* Source Type: research

Ascofuranone suppresses invasion and F-actin cytoskeleton organization in cancer cells by inhibiting the mTOR complex 1 signaling pathway
ConclusionsOur data indicate that ascofuranone suppresses IGF-1-induced cancer cell migration and invasion by blocking actin cytoskeleton organization and FAK activation through inhibition of the mTORC1 pathway, and reveal a novel anti-metastatic function of this compound.
Source: Cellular Oncology - June 2, 2020 Category: Cancer & Oncology Source Type: research

CARM1 promotes non-small cell lung cancer progression through upregulating CCNE2 expression.
Abstract The underlying molecular mechanisms of tumorigenesis and progression of non-small cell lung cancer (NSCLC) are not yet fully elucidated. In the present study, in vitro functional dissections suggest that siRNA-mediated silencing of CCNE2 profoundly attenuated the proliferative and colony-formative abilities of NSCLC PC9 and HCC827 cells, while forced overexpression of CCNE2 significantly strengthened the proliferative and colony-formative capabilities of these cells. Intriguingly, by ChIP and luciferase reporter gene assays, we observed that CARM1 is recruited to the promoter regions of CCNE2 gene and act...
Source: Aging - June 2, 2020 Category: Biomedical Science Authors: Wu D, He J, Zhang W, Wang K, Jin S, Li J, Gao W Tags: Aging (Albany NY) Source Type: research

Viral infection impacts transposable element transcript amounts in Drosophila Genetics
Transposable elements (TEs) are genomic parasites that are found in all genomes, some of which display sequence similarity to certain viruses. In insects, TEs are controlled by the Piwi-interacting small interfering RNA (piRNA) pathway in gonads, while the small interfering RNA (siRNA) pathway is dedicated to TE somatic control and...
Source: Proceedings of the National Academy of Sciences - June 2, 2020 Category: Science Authors: Marlene Roy, Barbara Viginier, Edouard Saint–Michel, Frederick Arnaud, Maxime Ratinier, Marie Fablet Tags: Biological Sciences Source Type: research

GSE144915 Transcriptional Responses to beta-catenin and FoxO with and without oxidative stress
Contributors : Leslie Gewin ; Yan GuoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo identify novel transcriptional targets of beta-catenin and FoxO1 and FoxO3 in renal epithelial cells, we used conditionally immortalized murine proximal tubule (PT) cells (these cells, from Tgfbr2floxed mice, are described in detail in manuscript PMID: 23160515 in which Leslie Gewin is first author, JASN 2012). PT cells were either treated with Wnt3a (to activate beta-catenin) or the control diluting buffer, H2O2 to induce oxidative stress, and some were transfected with FoxO1, FoxO3, FoxO1 and 3, ...
Source: GEO: Gene Expression Omnibus - June 1, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE103273 RNA-Seq of lymphoblastoid cell line (GM12878) upon siRNA mediated knockdown of Aiolos transcription factor.
Contributors : Christopher A Odhams ; Andrea Cortini ; Timothy J Vyse ; Deborah S Cunninghame GrahamSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe Ikaros family transcription factors Helios (IKZF2) and Aiolos (IKZF3), critical to lymphocyte homeostasis, are genetically associated with multiple immunological disorders including the prototypic autoimmune disease SLE. Given the lack of understanding between the association of Aiolos and its physiological function in humans, we determined their gene-regulatory pathways by RNA-Seq in the B-lymphocyte cell-line GM12878.
Source: GEO: Gene Expression Omnibus - June 1, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Hepatitis B virus X protein boosts hepatocellular carcinoma progression by downregulating microRNA-137.
CONCLUSION: Overexpression of miR-137 blocks HCC cell proliferation in HBx-siRNA-treated MHCC97H cells by targeting Notch1. This study may offer novel target for HCC treatment. PMID: 32527447 [PubMed - in process]
Source: Pathology, Research and Practice - June 1, 2020 Category: Pathology Authors: Gao Y, Gu J, Wang Y, Fu D, Zhang W, Zheng G, Wang X Tags: Pathol Res Pract Source Type: research

Effects of Sirt3 ‑autophagy and resveratrol activation on myocardial hypertrophy and energy metabolism.
In conclusion, Sirt3 may inhibit Ang II‑induced myocardial hypertrophy and reverse the Ang II‑caused abnormal myocardial energy metabolism through activation of autophagy. PMID: 32468001 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - May 31, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

GSE136091 Long-term EEF2 depletion in mouse liver leads to mTOR-independent translational upregulation of ribosomal genes
In this study, we were driven by the idea of perturbing protein synthesis directly without triggering mTOR signaling. We employed an in vivo siRNA treatment to suppress the translation elongation factor 2 (EEF2) in the liver of adult mice for the duration of up to 2 weeks. Proteomics and ribosome profiling assays showed strong translational upregulation of ribosomal proteins, which are known to be regulated by mTOR. At the same time, the branch of mTOR pathway responsible for translation regulation was not activated. We concluded that the upregulation of ribosomal transcripts occurred via the passive mTOR-independent mecha...
Source: GEO: Gene Expression Omnibus - May 31, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Other Mus musculus Source Type: research

Cancers, Vol. 12, Pages 1434: Combined Targeting of Glioblastoma Stem-Like Cells by Neutralizing RNA-Bio-Drugs for STAT3
o de Franciscis An important drawback in the management of glioblastoma (GBM) patients is the frequent relapse upon surgery and therapy. A likely explanation is that conventional therapies poorly affect a small population of stem-like cancer cells (glioblastoma stem cells, GSCs) that remain capable of repopulating the tumour mass. Indeed, the development of therapeutic strategies able to hit GSCs while reducing the tumour burden has become an important challenge to increase a patient’s survival. The signal transducer and activator of transcription-3 (STAT3) has been reported to play a pivotal role in main...
Source: Cancers - May 31, 2020 Category: Cancer & Oncology Authors: Carla Lucia Esposito Silvia Nuzzo Maria Luigia Ibba Lucia Ricci-Vitiani Roberto Pallini Gerolama Condorelli Silvia Catuogno Vittorio de Franciscis Tags: Article Source Type: research

Cancers, Vol. 12, Pages 1427: The Recombinant Fragment of Human κ-Casein Induces Cell Death by Targeting the Proteins of Mitochondrial Import in Breast Cancer Cells
In this study, mechanisms of RL2-induced cell death in breast cancer cells were systematically investigated. In particular, we demonstrate that RL2 induces loss of mitochondrial membrane potential and cellular ATP loss followed by cell death in breast cancer cells. The mass spectrometry-based screen for RL2 interaction partners identified mitochondrial import protein TOM70 as a target of RL2, which was subsequently validated. Further to this, we show that RL2 is targeted to mitochondria after internalization into the cells, where it can also be found in the dimeric form. The importance of TOM70 and RL2 interaction in RL2-i...
Source: Cancers - May 31, 2020 Category: Cancer & Oncology Authors: Max Richter Fabian Wohlfromm Thilo K ähne Hannes Bongartz Kamil Seyrek Yuriy Kit Olga Chinak Vladimir A. Richter Olga A. Koval Inna N. Lavrik Tags: Article Source Type: research

Ethanol Augments Monosodium Urate-Induced NLRP3 Inflammasome Activation via Regulation of AhR and TXNIP in Human Macrophages.
CONCLUSION: Ethanol stimulates uric acid-induced NLRP3 inflammasome activation through regression of AhR and upregulation of TXNIP. PMID: 32469177 [PubMed - in process]
Source: Yonsei Medical Journal - May 30, 2020 Category: Universities & Medical Training Authors: Kim SK, Choe JY, Park KY Tags: Yonsei Med J Source Type: research