Query: siRNA

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Condensin controls mitotic chromosome stiffness and stability without forming a structurally contiguous scaffold.
Abstract During cell division, chromosomes must be folded into their compact mitotic form to ensure their segregation. This process is thought to be largely controlled by the action of condensin SMC protein complexes on chromatin fibers. However, how condensins organize metaphase chromosomes is not understood. We have combined micromanipulation of single human mitotic chromosomes, sub-nanonewton force measurement, siRNA interference of condensin subunit expression, and fluorescence microscopy, to analyze the role of condensin in large-scale chromosome organization. Condensin depletion leads to a dramatic (~ 10-f...
Source: Chromosome Research - August 24, 2018 Category: Genetics & Stem Cells Authors: Sun M, Biggs R, Hornick J, Marko JF Tags: Chromosome Res Source Type: research

Ferulic Acid Protected from Kidney Ischemia Reperfusion Injury in Mice: Possible Mechanism Through Increasing Adenosine Generation via HIF-1 α
AbstractFerulic acid (FA), derived from fruits and vegetables, is well-known as a potent antioxidant of scavenging free radicals. However, the role and underlying mechanism of FA on kidney ischemia reperfusion (I/R) injury are limited. Here, we explored the effects of FA on kidney I/R injury. The kidney I/R injury models were carried out by clamping bilateral pedicles for 35  min followed by reperfusion for 24 h. Mice were orally pretreated with different doses of FA for three times 24 h before I/R. The renal function was assessed by serum creatine (Scr) and blood urea nitrogen (BUN). Kidney histology was examined by he...
Source: Inflammation - August 24, 2018 Category: Allergy & Immunology Source Type: research

MerTK Downregulates Lipopolysaccharide-Induced Inflammation Through SOCS1 Protein but Does Not Affect Phagocytosis of Escherichia coli in Macrophages
AbstractBacterial lipopolysaccharide (LPS) induces inflammatory responsevia toll-like receptor 4 (TLR4). However, this response must be strictly regulated because unbalanced overproduction of pro-inflammatory cytokines can lead to tissue damage and even be fatal. Herein, we explore whether Mer receptor tyrosine kinase (MerTK) regulatesEscherichia coli (E.coli) LPS-induced inflammation and mediates phagocytosis ofE.coli by macrophages. The results showed that LPS activated TLR4 signaling pathway and induced MerTK pathway in RAW264.7 macrophages, including suppressor of cytokine signaling1 (SOCS1). Preincubation with MerTK-s...
Source: Inflammation - August 24, 2018 Category: Allergy & Immunology Source Type: research

Effect of RNA Interference Targeting STAT3 Gene Combined with Ultrasonic Irradiation and SonoVue Microbubbles on Proliferation and Apoptosis in Keratinocytes of Psoriatic Lesions.
Conclusions: STAT3 siRNA inhibited the growth and induced the apoptosis in psoriatic KCs likely partly through altering cell cycle distribution, elevating [Ca2+]i, and downregulating cyclin D1 and Bcl-xL expressions. Silencing the target gene STAT3 in psoriatic KCs with siRNA combined with ultrasonic irradiation and microbubbles would contribute to a significant innovation as a new clinical therapy for psoriasis. PMID: 30127220 [PubMed - in process]
Source: Chinese Medical Journal - August 23, 2018 Category: General Medicine Authors: Ran LW, Wang H, Lan D, Jia HX, Yu SS Tags: Chin Med J (Engl) Source Type: research

Disruption of Planar Cell Polarity Pathway Attributable to Valproic Acid-Induced Congenital Heart Disease through Hdac3 Participation in Mice.
Conclusion: VPA could inhibit Hdac1/2/3, Vangl2/Scrib, or total Hdac activity both in vitro and in vivo and Hdac3 might participate in the process of VPA-induced cardiac developmental anomalies. PMID: 30127218 [PubMed - in process]
Source: Chinese Medical Journal - August 23, 2018 Category: General Medicine Authors: Duan HY, Zhou KY, Wang T, Zhang Y, Li YF, Hua YM, Wang C Tags: Chin Med J (Engl) Source Type: research

Chronic stress-associated visceral hyperalgesia correlates with severity of intestinal barrier dysfunction
In humans, chronic psychological stress is associated with increased intestinal paracellular permeability and visceral hyperalgesia, which is recapitulated in the chronic intermittent water avoidance stress (WAS) rat model. However, it is unknown whether enhanced visceral pain and permeability are intrinsically linked and correlate. Treatment of rats with lubiprostone during WAS significantly reduced WAS-induced changes in intestinal epithelial paracellular permeability and visceral hyperalgesia in a subpopulation of rats. Lubiprostone also prevented WAS-induced decreases in the epithelial tight junction protein, occludin ...
Source: Pain - August 23, 2018 Category: Anesthesiology Tags: Research Paper Source Type: research

Cancers, Vol. 10, Pages 283: CX-4945 Induces Methuosis in Cholangiocarcinoma Cell Lines by a CK2-Independent Mechanism
utamaad Satayavivad Cholangiocarcinoma is a disease with a poor prognosis and increasing incidence and hence there is a pressing unmet clinical need for new adjuvant treatments. Protein kinase CK2 (previously casein kinase II) is a ubiquitously expressed protein kinase that is up-regulated in multiple cancer cell types. The inhibition of CK2 activity using CX-4945 (Silmitasertib) has been proposed as a novel treatment in multiple disease settings including cholangiocarcinoma. Here, we show that CX-4945 inhibited the proliferation of cholangiocarcinoma cell lines in vitro. Moreover, CX-4945 treatment induced the formati...
Source: Cancers - August 23, 2018 Category: Cancer & Oncology Authors: Jomnarong Lertsuwan Kornkamon Lertsuwan Anyaporn Sawasdichai Nathapol Tasnawijitwong Ka Ying Lee Philip Kitchen Simon Afford Kevin Gaston Padma-Sheela Jayaraman Jutamaad Satayavivad Tags: Article Source Type: research

Calcineurin/NFAT Signaling Modulates Pulmonary Artery Smooth Muscle Cell Proliferation, Migration and Apoptosis in Monocrotaline-Induced Pulmonary Arterial Hypertension Rats
Conclusion: Our results demonstrate that CaN/NFAT signaling is activated and involved in the modulation of PASMC proliferation, migration and apoptosis in MCT-induced PAH.Cell Physiol Biochem 2018;49:172 –189
Source: Cellular Physiology and Biochemistry - August 22, 2018 Category: Cytology Source Type: research

HGF promotes HTR-8/SVneo cell migration through activation of MAPK/PKA signaling leading to up-regulation of WNT ligands and integrins that target β-catenin.
Abstract Inadequate migration and invasion of the trophoblast cells during embryo implantation is one of the reasons for pregnancy-related complications such as intrauterine growth restriction and preeclampsia. In the present study, relevance of WNT ligands and integrins associated with hepatocyte growth factor (HGF)-mediated migration of HTR-8/SVneo trophoblastic cells has been investigated. Treatment of HTR-8/SVneo cells with HGF led to a dose-dependent increase in their migration. RT-PCR studies revealed a significant increase in the transcripts of WNT4, WNT11, ITGA2, and ITGAV, which was further confirmed at p...
Source: Molecular and Cellular Biochemistry - August 22, 2018 Category: Biochemistry Authors: Chaudhary P, Malhotra SS, Babu GS, Sobti RC, Gupta SK Tags: Mol Cell Biochem Source Type: research

A novel allergen-specific therapy with CD40-silenced B cells and dendritic cells
CD40-silenced B cells, induced by siRNA, inhibited allergic responses and symptoms antigen-specifically even after allergic rhinitis had been established. CD40-silenced B cells and CD40-silenced dendritic cells also have independent mechanisms with a synergic effect.
Source: Journal of Allergy and Clinical Immunology - August 22, 2018 Category: Allergy & Immunology Authors: Motohiko Suzuki, Makoto Yokota, Yoshihisa Nakamura, Shinya Ozaki, Shingo Murakami Source Type: research

SslE (YghJ), a Cell-Associated and Secreted Lipoprotein of Neonatal Septicemic Escherichia coli, Induces Toll-Like Receptor 2-Dependent Macrophage Activation and Proinflammation through NF-{kappa}B and MAP Kinase Signaling Host Response and Inflammation
In this study, we showed that SslE specifically binds to Toll-like receptor 2 (TLR2)/TLR1 heterodimers and recruits downstream adaptors MyD88, TIRAP, and TRAF6. In addition, SslE stimulates nuclear translocation of NF-B and activates different mitogen-activated protein (MAP) kinase signaling cascades specific to the secretion of each cytokine in murine macrophages, which becomes impaired in TLR2 small interfering RNA (siRNA)-transfected cells and in cells blocked with a monoclonal antibody (MAb) against TLR2, suggesting the involvement of TLR2 in NF-B and MAP kinase activation and subsequent cytokine secretion. Furthermore...
Source: Infection and Immunity - August 22, 2018 Category: Infectious Diseases Authors: Tapader, R., Bose, D., Dutta, P., Das, S., Pal, A. Tags: Host Response and Inflammation Source Type: research

Combination therapy with KRAS siRNA and EGFR inhibitor AZD8931 suppresses lung cancer cell growth in vitro
Journal of Cellular Physiology, EarlyView.
Source: Journal of Cellular Physiology - August 22, 2018 Category: Cytology Authors: HabibZarredar , ShadiPashapour , KhalilAnsarin , MajidKhalili , RoghayyehBaghban , SafarFarajnia Source Type: research

STAT3 promotes tumour progression in glioma by inducing FOXP1 transcription.
CONCLUSION: In summary, STAT3 gene was a transcriptional regulator of FOXP1. Depleted STAT3 restrained cell proliferation and invasion, promoted cell apoptosis in glioma cells. This molecular mechanism between STAT3 and FOXP1 can serve as a therapeutic target for glioma treatment. PMID: 30134017 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - August 22, 2018 Category: Molecular Biology Authors: Sun X, Wang J, Huang M, Chen T, Chen J, Zhang F, Zeng H, Xu Z, Ke Y Tags: J Cell Mol Med Source Type: research

Down-regulated in OA cartilage, SFMBT2 contributes to NF- κB-mediated ECM degradation.
Down-regulated in OA cartilage, SFMBT2 contributes to NF-κB-mediated ECM degradation. J Cell Mol Med. 2018 Aug 22;: Authors: Hussain S, Sun M, Min Z, Guo Y, Xu J, Mushtaq N, Heng L, Huang H, Zhao Y, Yuan Y, Hussain N, Zhang F, Han Y, Xu P, Sun J, Lu S Abstract The interplay between anabolic and catabolic factors regulates cartilage matrix homoeostasis. In OA, this balance is disrupted which results in cartilage degradation involving a plethora of inflammatory factors. Here, we identify a novel gene "Scm-like with four MBT domains protein 2" (SFMBT2) negatively regulated in OA cartilage. Articular car...
Source: J Cell Mol Med - August 22, 2018 Category: Molecular Biology Authors: Hussain S, Sun M, Min Z, Guo Y, Xu J, Mushtaq N, Heng L, Huang H, Zhao Y, Yuan Y, Hussain N, Zhang F, Han Y, Xu P, Sun J, Lu S Tags: J Cell Mol Med Source Type: research

Long non-coding RNA NEAT1 promotes proliferation, migration and invasion of human osteosarcoma cells.
Conclusion: LncRNA NEAT1 was up-regulated in osteosarcoma tissue, promoting proliferation and metastasis of osteosarcoma cells. These findings indicate the role of this substance, as a growth regulator in osteosarcoma, and thus it may serve as a novel biomarker, and drug target for developing osteosarcoma therapies. PMID: 30123061 [PubMed - in process]
Source: International Journal of Medical Sciences - August 21, 2018 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Axis of serotonin -pERK-YAP in liver regeneration
ConclusionA role of the 5-HT-pERK-YAP axis in liver regeneration emerged from our study and might be a potential target to promote regeneration and injury repair.
Source: Life Sciences - August 21, 2018 Category: Biology Source Type: research

Small Interfering RNA based knock down of acute heat shock and or GGA inducible bovine heat shock protein 70 may interfere invitro expression pattern of TLR2/4 and NOD1/2
This study may add some references pertaining to the innate immune modulatory effects of bovine heat shock protein 70.
Source: Journal of Thermal Biology - August 21, 2018 Category: Biology Source Type: research

GSE107469 Transcriptome analysis of human endometrial stromal cells with NR2F2 (COUP-TFII) knockdown
Contributors : Shaw-Jenq Tsai ; Kuei-Yang HsiaoSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo explore the roles of COUP-TFII during the pathogenesis of endometriosis, the human eutopic stromal cells were transfected with siRNA oligonucleotide against COUP-TFII, and total RNA were harvested and proceeded to microarray analysis.
Source: GEO: Gene Expression Omnibus - August 21, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

PCB 95 promotes dendritic growth in primary rat hippocampal neurons via mTOR-dependent mechanisms.
Abstract Polychlorinated biphenyls (PCBs), and in particular non-dioxin-like (NDL) congeners, continue to pose a significant risk to the developing nervous system. PCB 95, a prevalent NDL congener in the human chemosphere, promotes dendritic growth in rodent primary neurons by activating calcium-dependent transcriptional mechanisms that normally function to link activity to dendritic growth. Activity-dependent dendritic growth is also mediated by calcium-dependent translational mechanisms involving mechanistic target of rapamycin (mTOR), suggesting that the dendrite-promoting activity of PCB 95 may also involve mT...
Source: Archives of Toxicology - August 21, 2018 Category: Toxicology Authors: Keil KP, Miller GW, Chen H, Sethi S, Schmuck MR, Dhakal K, Kim JW, Lein PJ Tags: Arch Toxicol Source Type: research

Role of retinal pigment epithelium-derived exosomes and autophagy in new blood vessel formation.
Abstract Autophagy and exosome secretion play important roles in a variety of physiological and disease states, including the development of age-related macular degeneration. Previous studies have demonstrated that these cellular mechanisms share common pathways of activation. Low oxidative damage in ARPE-19 cells, alters both autophagy and exosome biogenesis. Moreover, oxidative stress modifies the protein and genetic cargo of exosomes, possibly affecting the fate of surrounding cells. In order to understand the connection between these two mechanisms and their impact on angiogenesis, stressed ARPE-19 cells were ...
Source: J Cell Mol Med - August 21, 2018 Category: Molecular Biology Authors: Atienzar-Aroca S, Serrano-Heras G, Freire Valls A, Ruiz de Almodovar C, Muriach M, Barcia JM, Garcia-Verdugo JM, Romero FJ, Sancho-Pelluz J Tags: J Cell Mol Med Source Type: research

Immunization with Na+/K+ ATPase DR peptide prevents bone loss in an ovariectomized rat osteoporosis model.
Abstract Osteoporosis is characterized by decreased bone strength and microarchitectural deterioration of bone tissue leading to an increase in bone fracture. Here we report a new agent named DR peptide, a conserved sequence of Na+/K+ ATPase (NKA), can be used to prevent osteoporosis. Our results showed that immunization with DR peptide conjunct with Keyhole limpet hemocyanin (DR-KLH) significantly strengthened trabecular bone and improved bone mineral density of femur and the 5th lumbar in the ovariectomized (OVX) rats when compared with those in OVX rats immunized with KLH alone. To study the underlying mechanis...
Source: Biochemical Pharmacology - August 19, 2018 Category: Drugs & Pharmacology Authors: Xiong S, Yang X, Yan X, Hua F, Zhu M, Guo L, Wu Z, Bian JS Tags: Biochem Pharmacol Source Type: research

Scavenger Receptor Class BI (SR-BI) Mediates Uptake of CPX-351 into a K562 Leukemia Cells.
CONCLUSIONS: These preliminary studies suggest that SR-BI may be one potential mechanism by which CPX-351 is taken up into K562 cells. PMID: 30113235 [PubMed - as supplied by publisher]
Source: Drug Development and Industrial Pharmacy - August 18, 2018 Category: Drugs & Pharmacology Tags: Drug Dev Ind Pharm Source Type: research

Role of vasodilator-stimulated phosphoprotein in human cytomegalovirus-induced hyperpermeability of human endothelial cells.
Authors: Tian Y, He Y, Zhang L, Zhang J, Xu L, Ma Y, Xu X, Wei L Abstract Atherosclerosis (AS) is a common chronic vascular disease and epidemiological evidence demonstrates that infection is closely associated with the occurrence of AS, including infection by human cytomegalovirus (HCMV) and Chlamydophila pneumoniae. The aim of the present study was to investigate the effect of HCMV AD169 infection on the barrier function of human umbilical vein endothelial cells (HUVECs) and to understand the role of vasodilator-stimulated phosphoprotein (VASP) during this process. In cultured HUVEC-CRL-1730 cells, knockdown of V...
Source: Experimental and Therapeutic Medicine - August 18, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced-podocytes.
Authors: Cai X, Wang L, Wang X, Hou F Abstract Insulin-like growth factor-binding protein 7 (IGFBP7) has been identified as a secreted protein associated with a number of cellular processes. However, the specific regulatory mechanisms of IGFBP7 on podocytes of diabetic nephropathy (DN) are yet to be elucidated. In the present study, podocytes were identified initially via an immunofluorescence assay using an anti-synaptopodin antibody. It was subsequently demonstrated that glucose promoted podocyte proliferation in a time- and dose-dependent manner via MTT assay. In addition, IGFBP7 expression was silenced in podoc...
Source: Experimental and Therapeutic Medicine - August 18, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Transient inhibition of sphingosine kinases confers protection to influenza A virus infected mice
Publication date: Available online 17 August 2018Source: Antiviral ResearchAuthor(s): Chuan Xia, Young-Jin Seo, Caleb J. Studstill, Madhuvanthi Vijayan, Jennifer J. Wolf, Bumsuk HahmAbstractInfluenza continues to pose a threat to public health by causing illness and mortality in humans. Discovering host factors that regulate influenza virus propagation is vital for the development of novel drugs. We have previously reported that sphingosine kinase (SphK) 1 promotes influenza A virus (IAV) replication in vitro. Here we demonstrate that the other isoform of SphK, SphK2 promotes the replication of influenza A virus (IAV) in c...
Source: Antiviral Therapy - August 18, 2018 Category: Virology Source Type: research

Rosiglitazone augments antioxidant response in the human trophoblast and prevents apoptosis.
In this study, we first examined whether rosiglitazone, an agonist of the peroxisome proliferator-activated receptor-γ (PPARγ), protects the human trophoblast from oxidative injury by regulating key antioxidant proteins, catalase (CAT) and the superoxide dismutases (SOD1 and SOD2). In first trimester placental explants, localization of CAT was limited to cytotrophoblasts, whereas SOD1 was expressed in both the cyto- and syncytiotrophoblasts. In first trimester placental explants, hypoxia decreased the expression of both SOD1 and SOD2, and increased apoptosis. Treatment with rosiglitazone dose-dependently upregulated anti...
Source: Biology of Reproduction - August 18, 2018 Category: Reproduction Medicine Authors: Kohan-Ghadr HR, Kilburn BA, Kadam L, Johnson E, Kolb BL, Rodriguez-Kovacs J, Hertz M, Armant DR, Drewlo S Tags: Biol Reprod Source Type: research

HMGA2 is regulated by LIN28 and BRCA1 in human placental cells †.
HMGA2 is regulated by LIN28 and BRCA1 in human placental cells†. Biol Reprod. 2018 Aug 18;: Authors: West RC, McWhorter ES, Ali A, Goetzman LN, Russ JE, Anthony RV, Bouma GJ, Winger QA Abstract The chromatin associated transcription factor HMGA2 is a downstream target of let-7 miRNAs and binds to chromatin to regulate gene expression. Inhibition of let-7 miRNAs by RNA binding proteins LIN28A and LIN28B is necessary during early embryogenesis to ensure stable expression of HMGA2. In addition to LIN28, HMGA2 is regulated by a BRCA1/ZNF350/CtIP repressor complex. In normal tissues, the BRCA1/ZNF350/CtI...
Source: Biology of Reproduction - August 18, 2018 Category: Reproduction Medicine Authors: West RC, McWhorter ES, Ali A, Goetzman LN, Russ JE, Anthony RV, Bouma GJ, Winger QA Tags: Biol Reprod Source Type: research

TMEM119 silencing inhibits cell viability and causes the apoptosis of gastric cancer SGC-7901 cells.
Authors: Zheng P, Wang W, Ji M, Zhu Q, Feng Y, Zhou F, He Q Abstract Gastric cancer is the second major cause of death associated with cancer and ranks among the top four cancers diagnosed worldwide. Previous findings identified the association of transmembrane proteins (TMEMs) with tumorigenesis of various types of cancer, including breast, liver and kidney cancer. However, the expression and the biological function of TMEMs, especially TMEM119, and its possible molecular mechanism in gastric cancer remain less understood. CCK-8 and flow cytometric analysis was employed to examine the viability and apoptosis of ga...
Source: Oncology Letters - August 17, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Shenxian-Shengmai Oral Liquid Reduces Myocardial Oxidative Stress and Protects Myocardium from Ischemia-Reperfusion Injury
Conclusion: SXSM is effective in protecting the myocardium from I/R injury, with myocardial SOD1 and GCLC being the potential therapeutic targets.Cell Physiol Biochem 2018;48:2503 –2516
Source: Cellular Physiology and Biochemistry - August 17, 2018 Category: Cytology Source Type: research

Autophagy-dependent apoptosis is triggered by a semi-synthetic 6-gingerol analogue in triple negative breast cancer cells.
In this study, we show that the semi-synthetic analogue SSi6, generated after chemical modification of the [6]-gingerol molecule, using acetone-2,4-dinitrophenylhydrazone (2,4-DNPH) reagent, enhanced selective cytotoxic effects on MDA-MB-231 cells. Remarkably, unlike the original [6]-gingerol molecule, SSi6 enabled autophagy followed by caspase-independent apoptosis in tumor cells. We found a time-dependent association between SSi6-induced oxidative stress, autophagy and apoptosis. Initial SSi6-induced reactive oxygen species (ROS) accumulation (1h) led to autophagy activation (2-6h), which was followed by caspase-independ...
Source: Oncotarget - August 17, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

High Mobility Group Box 1 Regulates Uterine Decidualization through Bone Morphogenetic Protein 2 and Plays a Role in Kruppel-Like Factor 5-Induced Stromal Differentiation
Conclusion: Hmgb1 may play an important role during mouse uterine decidualization.Cell Physiol Biochem 2018;48:2399 –2408
Source: Cellular Physiology and Biochemistry - August 17, 2018 Category: Cytology Source Type: research

Study on Regulation of Low Density Lipoprotein Cholesterol Metabolism using PCSK9 Gene Silencing: A computational Approach.
Abstract Combating and preventing abnormality in lipid metabolism becomes a pivotal criterion for research. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a circulating protein; it promotes the degradation of low-density lipoprotein receptors (LDL-R) and hence increases LDL-C levels. Silencing the gene PCSK9 at post-transcriptional level with the help of small interfering Ribo nucleic acid (siRNA) gives a new insight and a novel therapeutic way to regulate LDL-C metabolism. Designing and selecting an efficient siRNA for silencing PCSK9 at post transcriptional level through computational approach. We have...
Source: Bioinformation - August 17, 2018 Category: Bioinformatics Authors: Vijayaraghavan B, Danabal K, Padmanabhan G, Ramanathan K Tags: Bioinformation Source Type: research

ASAP Near-Infrared Photothermal Release of siRNA from the Surface of Colloidal Gold –Silver–Gold Core–Shell–Shell Nanoparticles Studied with Second-Harmonic Generation
The Journal of Physical Chemistry CDOI: 10.1021/acs.jpcc.8b06117
Source: The Journal of Physical Chemistry C - August 17, 2018 Category: Chemistry Authors: Raju R. Kumal, Mohammad Abu-Laban, Prakash Hamal, Blake Kruger, Holden T. Smith, Daniel J. Hayes, Louis H. Haber Source Type: research

GSE118642 ANXA10 induction by interaction with tumor-associated macrophages promotes the growth of esophageal squamous cell carcinoma
Contributors : Himiko Kodaira ; Hiroshi YokozakiSeries Type : Expression profiling by arrayOrganism : Homo sapiensTumor-associated macrophages (TAMs) have important roles in the progression, angiogenesis and motility of various cancers. To study the effects of TAMs on the tumor microenvironment of ESCCs, we constructed an in vitro system for the differentiation of peripheral blood monocyte (PBMo)-derived macrophages into TAM-like PBMo-derived macrophages.We established a co-culture assay using a human ESCC cell line and TAM-like PBMo-derived macrophages and we performed a cDNA microarray analysis between monocultured and c...
Source: GEO: Gene Expression Omnibus - August 17, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE113369 CDK4 inhibition diminishes p53 activation by Mdm2-antagonists
Contributors : Matthias Dobbelstein ; Anusha SriramanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCDK4 inhibitors have reached clinical approval for cancer therapy. In parallel, the p53 antagonist Mdm2 remains an attractive target for anti-cancer therapy, including numerous clinical studies. The genes encoding Mdm2 and CDK4 are frequently co-amplified in human malignancies, most notably in liposarcomas, suggesting their combined targeting for therapy. Here we show, however, that small compounds that inhibit Mdm2 and CDK4 antagonize each other rather than synergize in their cytotoxi...
Source: GEO: Gene Expression Omnibus - August 17, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Cloning, expression analysis and functional characterization of an interleukin-1 receptor-associated kinase 4 from Apostichopus japonicus
In this study, an IRAK4 homologue molecule was identified from Apostichopus japonicus (designated as AjIRAK4) by RACE approach. The full-length cDNA of AjIRAK4 was of 2024 bp containing an open reading frame of 1311 bp encoding a 436-amino-acid (aa) residue polyprotein with the typical death domain (10-113aa) and the kinase domain (160-426aa). The mRNA transcripts of AjIRAK4 displayed constitutively expressed in all examined tissues with highest expression in the muscles (7.20-fold increase compared to the coelomocytes). The pathogen Vibrio splendidus challenge and LPS exposure could both significantly up-regulate the mRNA...
Source: Molecular Immunology - August 17, 2018 Category: Allergy & Immunology Source Type: research

Bradykinin-mediated Ca2+ signalling regulates cell growth and mobility in human cardiac c-Kit+ progenitor cells.
This study investigated whether bradykinin-mediated Ca2+ signalling participates in regulating cellular functions in cultured human cardiac c-Kit+ progenitor cells using laser scanning confocal microscopy and biochemical approaches. It was found that bradykinin increased cytosolic free Ca2+ (Cai2+) by triggering a transient Ca2+ release from ER IP3Rs followed by sustained Ca2+ influx through store-operated Ca2+ entry (SOCE) channel. Blockade of B2 receptor with HOE140 or IP3Rs with araguspongin B or silencing IP3R3 with siRNA abolished both Ca2+ release and Ca2+ influx. It is interesting to note that the bradykinin-induced...
Source: J Cell Mol Med - August 17, 2018 Category: Molecular Biology Authors: Li G, Che H, Wu WY, Jie LJ, Xiao GS, Wang Y, Li GR Tags: J Cell Mol Med Source Type: research

IFN- γ-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD.
IFN-γ-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD. Biochem J. 2018 Aug 17;: Authors: Unterer B, Wiesmann V, Gunasekaran M, Sticht H, Tenkerian C, Behrens J, Leone M, Engel FB, Britzen-Laurent N, Naschberger E, Wittenberg T, Stürzl M Abstract Interferon-gamma (IFN‑γ) is a pleiotropic cytokine that exerts important functions in inflammation, infectious diseases and cancer. The large GTPase human guanylate-binding protein 1 (GBP‑1) is among the most strongly IFN‑γ-induced cellular proteins. Previously it has been shown t...
Source: The Biochemical Journal - August 17, 2018 Category: Biochemistry Authors: Unterer B, Wiesmann V, Gunasekaran M, Sticht H, Tenkerian C, Behrens J, Leone M, Engel FB, Britzen-Laurent N, Naschberger E, Wittenberg T, Stürzl M Tags: Biochem J Source Type: research

Transient inhibition of sphingosine kinases confers protection to influenza A virus infected mice.
Abstract Influenza continues to pose a threat to public health by causing illness and mortality in humans. Discovering host factors that regulate influenza virus propagation is vital for the development of novel drugs. We have previously reported that sphingosine kinase (SphK) 1 promotes influenza A virus (IAV) replication in vitro. Here we demonstrate that the other isoform of SphK, SphK2 promotes the replication of influenza A virus (IAV) in cultured cells, and temporary inhibition of SphK1 or SphK2 enhances the host defense against influenza in mice. IAV infection led to an increased expression and phosphorylat...
Source: Antiviral Research - August 17, 2018 Category: Virology Authors: Xia C, Seo YJ, Studstill CJ, Vijayan M, Wolf JJ, Hahm B Tags: Antiviral Res Source Type: research

Betulinic acid alleviates myocardial hypoxia/reoxygenation injury via inducing Nrf2/HO-1 and inhibiting p38 and JNK pathways.
Abstract Myocardial ischemia reperfusion injury (I/RI) can lead to acute myocardial infarction (MI), which is a common cardiovascular disease (CVD) leading to morbidity and mortality worldwide. Betulinic acid exerts protective effect on myocardial I/RI in rats; however, the mechanism remains unknown. The aim of the current study was to evaluate the effect of betulinic acid on myocardial I/RI in vitro and further investigate the underlying mechanism. Our results showed that betulinic acid increased cell viability and decreased lactate dehydrogenase (LDH) release in H9c2 cells exposed to H/R. Betulinic acid was foun...
Source: European Journal of Pharmacology - August 17, 2018 Category: Drugs & Pharmacology Authors: Wang D, Chen T, Liu F Tags: Eur J Pharmacol Source Type: research

IRF5 is increased in labouring myometrium and regulates pro-labour mediators
Preterm birth continues to be the leading cause of neonatal mortality and morbidities that can extend into adult life. Few treatment options stem from our incomplete understanding of the mechanisms of human labour and delivery. Activation of the inflammatory response in gestational tissues by inflammation and/or infection leads to the production of pro-inflammatory and pro-labour mediators, thus preterm birth. Interferon regulatory factor 5 (IRF5) has recently emerged as an important pro-inflammatory transcription factor involved in acute and chronic inflammation. The aims of this study were to determine the expression of ...
Source: Reproduction - August 17, 2018 Category: Reproduction Medicine Authors: Lim, R., Barker, G., Lappas, M. Tags: Research Source Type: research

Periostin promotes liver fibrogenesis by activating lysyl oxidase in hepatic stellate cells Molecular Bases of Disease
Liver fibrosis arises from dysregulated wound healing due to persistent inflammatory hepatic injury. Periostin is a nonstructural extracellular matrix protein that promotes organ fibrosis in adults. Here, we sought to identify the molecular mechanisms in periostin-mediated hepatic fibrosis. Hepatic fibrosis in periostin−/− mice was attenuated as evidenced by significantly reduced collagen fibril density and liver stiffness compared with those in WT controls. A single dose of carbon tetrachloride caused similar acute liver injury in periostin−/− and WT littermates, and we did not detect significant differences in tr...
Source: Journal of Biological Chemistry - August 17, 2018 Category: Chemistry Authors: Pradeep Kumar, Tekla Smith, Reben Raeman, Daniel M. Chopyk, Hannah Brink, Yunshan Liu, Todd Sulchek, Frank A. Anania Tags: Cell Biology Source Type: research

Ethanol Extract of Lycopus lucidus Turcz. ex Benth Inhibits Metastasis by Downregulation of Runx-2 in Mouse Colon Cancer Cells.
This study aims to elucidate the effects of ethanol extracts of LT (ELT) relative to the role of Runt-related transcription factor- (Runx-) 2 in the invasive and metastatic potentials of mouse colon cancer to determine the underlying mechanisms involved. ELT was evaluated for the antimetastasis activity using CT-26 colon cancer using wound healing, transwell matrigel, and western blot analysis. We used Runx-2-specific siRNA to further determine the relationship between Runx-2 and matrix metalloprotease- (MMP-) 9 in the migration and invasion of CT-26 cells. Runx-2 was first demonstrated to be a transcription factor that pl...
Source: Evidence-based Complementary and Alternative Medicine - August 16, 2018 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

Beta-secretase/BACE1 Promotes APP Endocytosis and Processing in the Endosomes and on Cell Membrane
Publication date: Available online 16 August 2018Source: Neuroscience LettersAuthor(s): Mingguang Wang, Tian Jing, Xuan Wang, Dan YaoAbstractAmyloid-β proteins deposition and aggregation occur in extracellular space and form neuritic plaques in Alzheimer’s disease (AD) brain. Beta-site amyloid precursor protein cleaving enzyme 1 (BACE1)/ β-secretase and γ-secretase Presenilin 1 (PSEN1) conduct sequential cleavage of amyloid- β precursor protein (APP) and yield amyloid- β proteins. However the details of the interactions of APP with the enzymes and transportation of catalytic products are unclear. Here we reveal dist...
Source: Neuroscience Letters - August 16, 2018 Category: Neuroscience Source Type: research

ASAP pH-Sensitive Nanocarrier-Mediated Codelivery of Simvastatin and Noggin siRNA for Synergistic Enhancement of Osteogenesis
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b10521
Source: ACS Applied Materials and Interfaces - August 16, 2018 Category: Materials Science Authors: Jinsheng Huang, Chaowen Lin, Jintao Fang, Xiaoxia Li, Jin Wang, Shaohui Deng, Sheng Zhang, Wanhan Su, Xiaoreng Feng, Bin Chen, Du Cheng, Xintao Shuai Source Type: research

miR ‑197 promotes the invasion and migration of colorectal cancer by targeting insulin‑like growth factor‑binding protein 3.
In conclusion, we revealed that miR‑197 modulates IGFBP3 and therefore plays a critical role in regulating CRC migration and invasion. PMID: 30106114 [PubMed - as supplied by publisher]
Source: Oncology Reports - August 16, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Inhibitor of apoptosis protein ‑like protein‑2: A novel growth accelerator for breast cancer cells.
Inhibitor of apoptosis protein‑like protein‑2: A novel growth accelerator for breast cancer cells. Oncol Rep. 2018 Aug 03;: Authors: Zhu L, Zhou W, Zhu X, Xiang S, Wang S, Peng Y, Lu B, Tang P, Chen Q, Wu M, Peng X, Chen Z, Sun Z, Yang K, Xiang M, Yu D Abstract Although the inhibitor of apoptosis protein‑like protein‑2 (ILP‑2) has been shown as a serological biomarker for breast cancer, its effect on breast cancer cell growth remains elusive. The present study aimed to determine the role of ILP‑2 in breast cancer cell growth. We used immunohistochemistry to analyze ILP‑2 expression in 59 ...
Source: Oncology Reports - August 16, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MicroRNA ‑34b promotes proliferation, migration and invasion of Ewing's sarcoma cells by downregulating Notch1.
In conclusion, EWS‑FLI1 may modulate miR‑34b expression directly or indirectly, and miR‑34b potentially has an oncogenic role in Ewing's sarcoma by downregulating Notch1. PMID: 30106161 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - August 16, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Correlation between DEC1/DEC2 and epithelial ‑mesenchymal transition in human prostate cancer PC‑3 cells.
Correlation between DEC1/DEC2 and epithelial‑mesenchymal transition in human prostate cancer PC‑3 cells. Mol Med Rep. 2018 Aug 09;: Authors: Liu Q, Wu Y, Seino H, Haga T, Yoshizawa T, Morohashi S, Kijima H Abstract Differentiated embryonic chondrocyte (DEC) genes have been reported to be involved in the regulation of mammalian circadian rhythms, differentiation, apoptosis, the response to hypoxia and epithelial‑mesenchymal transition (EMT). Activation of transforming growth factor (TGF)‑β signaling is known to promote EMT for the development of metastatic castration‑resistant prostate cancer...
Source: Molecular Medicine Reports - August 16, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

GSE98514 Expression profiling of four replicates of MCF-7 cells treated with 100nM TCDD
Contributors : Sean Piwarski ; James Denvir ; Donald A Primerano ; Jun Fan ; Travis B SalisburySeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that binds pollutants, therapeutic drugs and endogenous ligands. The AHR is expressed in all breast cancer subtypes and it can switch the aggressiveness of breast cancer cells from low to high depending on the ligand that it binds. Jagged 1 (JAG1) is a NOTCH receptor ligand that is overexpressed in basal-like breast cancer. JAG1 promotes breast cancer progression in ...
Source: GEO: Gene Expression Omnibus - August 15, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research