miR-124/IRE-1 α affects renal ischemia/reperfusion injury by regulating endoplasmic reticulum stress in renal tubular epithelial cells.
In conclusion, our data indicated that miR-124 was a negative regulator of ERS via binding to IRE-1α, ultimately conferring its protective effect on the kidney, which demonstrates the regulatory mechanism of miR-124 in renal I/R injury and provides new ideas and methods for the prevention and treatment of renal I/R injury. PMID: 31965139 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - January 22, 2020 Category: Biochemistry Authors: Ding C, Dou M, Wang Y, Li Y, Wang Y, Zheng J, Li X, Xue W, Ding X, Tian P Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Gene expression profiling of cells of origin of squamous cell carcinomas in head-and-neck, esophagus, and lung.
PMID: 31960064 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - January 21, 2020 Category: Biochemistry Authors: Qu N, Huang D, Xu Q, Wang J, Chen C, Ji Q, Wu W, Zhu Y, Xu M Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Cordycepin protects renal ischemia/reperfusion injury through regulating inflammation, apoptosis, and oxidative stress.
Abstract Cordycepin (3'-deoxyadenosine) is a naturally occurring adenosine analog and one of the bioactive constituents isolated from Cordyceps sinensis, species of the fungal genus Cordyceps. It has traditionally been a prized Chinese folk medicine for the human well-being. However, the actions of cordycepin against renal ischemia/reperfusion injury (I/R) are still unknown. In the present study, rats were subject to I/R and cordycepin was intragastrically administered for seven consecutive days before surgery to investigate the effects and mechanisms of cordycepin against renal I/R injury. The test results of kid...
Source: Acta Biochimica et Biophysica Sinica - January 17, 2020 Category: Biochemistry Authors: Han F, Dou M, Wang Y, Xu C, Li Y, Ding X, Xue W, Zheng J, Tian P, Ding C Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

IL-9 blockade attenuates inflammation in a murine model of methicillin-resistant Staphylococcus aureus pneumonia.
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is an important etiology of pneumonia. Interleukin (IL)-9 is a T helper 9 (Th9) cytokine and participates in the pathogenesis of infectious diseases. Here, we investigated the role of IL-9 by using an MRSA pneumonia animal model. The BALB/c mice underwent nasal inhalation with an ST239 MRSA strain to establish the mouse model of MRSA pneumonia, and a subset of mice were intravenously injected with IL-9 neutralizing antibody or immunoglobulin (Ig) G. At 3 and 8 days postinfection, the peripheral blood, bronchioalveolar lavage fluid (BALF), and lung ti...
Source: Acta Biochimica et Biophysica Sinica - January 15, 2020 Category: Biochemistry Authors: Xu W, Tian K, Li X, Zhang S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Downregulating integrin subunit alpha 7 (ITGA7) promotes proliferation, invasion, and migration of papillary thyroid carcinoma cells through regulating epithelial-to-mesenchymal transition.
In conclusion, these results indicated that ITGA7 is involved in the progress of PTC and might act as a tumor suppressor gene. PMID: 31942970 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - January 14, 2020 Category: Biochemistry Authors: Guan Y, Bhandari A, Xia E, Kong L, Zhang X, Wang O Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Long noncoding RNA UC.98 stabilizes atherosclerotic plaques by promoting the proliferation and adhesive capacity in murine aortic endothelial cells.
In conclusion, our results highlighted the pivotal role of UC.98 in regulating the vulnerability of plaques during AS progression and suggested that UC.98 may be a biomarker of the early diagnosis and prognosis of AS with vulnerable plaques and a potential therapeutic target for slowing AS progression. PMID: 31942916 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - January 11, 2020 Category: Biochemistry Authors: Fan Z, Zhang Y, Xiao D, Ma J, Liu H, Shen L, Zhang M, He B Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Enhanced store-operated calcium entry in platelets is associated with acute coronary syndrome.
PMID: 31942931 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - January 11, 2020 Category: Biochemistry Authors: Guo X, Zhang J, Zhu J, Chen QH, Wang R, Gui L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

MiR-155 aggravates impaired autophagy of pancreatic acinar cells through targeting Rictor.
Abstract The aim of this study was to investigate the role and mechanism of miR-155 in regulating autophagy in a caerulein-induced acute pancreatitis (AP) cellular model. GFP-LC3 immunofluorescence assay was performed to detect autophagy vesicle formation in pancreatic acinar cell line AR42J. AR42J cells were transfected with miR-155 mimic, inhibitor, and corresponding controls to explore the effect of miR-155 on autophagy. The protein levels of LC3-I, LC3-II, Beclin-1, and p62 were analyzed by western blot analysis. Dual-luciferase reporter assay was performed to verify the interaction between miR-155 and Rictor ...
Source: Acta Biochimica et Biophysica Sinica - January 11, 2020 Category: Biochemistry Authors: Zhang X, Chu J, Sun H, Zhao D, Ma B, Xue D, Zhang W, Li Z Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

miR-223-3p promotes cell proliferation and invasion by targeting Arid1a in gastric cancer.
In conclusion, our results demonstrated that miR-223-3p inhibits gastric cancer cell progression by decreasing the expression of Arid1a. Therefore, miR-223-3p may act as a potential therapeutic target for patients with gastric cancer. PMID: 31912865 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - January 8, 2020 Category: Biochemistry Authors: Zhu Y, Li K, Yan L, He Y, Wang L, Sheng L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Betulinic acid induces apoptosis of gallbladder cancer cells via repressing SCD1.
In this study, we investigated the effect of BetA on five GBC cell lines and found that BetA significantly inhibited the proliferation of NOZ cells but had little inhibitory effect on other GBC cells. BetA disturbed mitochondrial membrane potential and induced apoptosis in NOZ cells. Real-time polymerase chain reaction analysis revealed that stearoyl-coenzyme A desaturase 1 (SCD1) was highly expressed in NOZ cells but low expressed in other GBC cells. BetA inhibited SCD1 expression in a concentration-dependent manner in NOZ cells. Downregulation of SCD1 expression by RNA interference inhibited the proliferation of NOZ cell...
Source: Acta Biochimica et Biophysica Sinica - January 8, 2020 Category: Biochemistry Authors: Wang H, Dong F, Wang Y, Wang X, Hong D, Liu Y, Zhou J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

MCU deficiency blocks cell proliferation by disrupting cytoplasmic Ca2+ transients due to altered Drp1 hospitalization.
PMID: 31907514 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - January 7, 2020 Category: Biochemistry Authors: Wang L, Zhang K, Fu N, Li L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Exogenous abscisic acid suppresses the lignification and changes the growth, root anatomical structure and related gene profiles of carrot.
PMID: 31897466 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - January 3, 2020 Category: Biochemistry Authors: Khadr A, Wang Y, Que F, Li T, Xu Z, Xiong A Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Interrelationship between Alzheimer's disease and cardiac dysfunction: the brain-heart continuum?
Conclusion: Due to the increased incidence of dementia and cardiovascular diseases, the coexistence of the two will cause more threat to public health, warranting much more attention. Here, we will update recent reports on dementia, AD, and cardiovascular diseases and discuss the causal relationship between dementia and heart dysfunction. PMID: 31897470 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - January 3, 2020 Category: Biochemistry Authors: Yang M, Li C, Zhang Y, Ren J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Release of endogenous hydrogen sulfide in enteric nerve cells suppresses intestinal motility during severe acute pancreatitis.
In this study, we examined the association between SAP-induced H2S release and loss of intestinal motility, and also explored the relevant mechanism in enteric nerve cells. A rat SAP model was constructed and enteric nerve cells were prepared. Intestinal mobility was evaluated by measuring the number of bowel movements at indicated time points and by performing intestinal propulsion tests. The production of inflammatory cytokines during a SAP attack was quantified by ELISA, and the levels of cystathionine-γ-lyase (CSE) and cystathionine-β-synthase (CBS) were examined by immunohistochemistry and western blot anal...
Source: Acta Biochimica et Biophysica Sinica - December 31, 2019 Category: Biochemistry Authors: Liao R, Xue L, Qiang Z, Zhang C, Liu Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

In vivo acute toxicity and anti-gastric evaluation of a novel dichloro Schiff base: Bax and HSP70 alteration.
Abstract Chlorine is shown to possess anti-gastric ulcer activity, since it can inactivate Helicobacter pylori, which is regarded as one of the most common risk factors for causing gastric problems. In the current study, the gastroprotective property of a novel dichloro-substituted Schiff base complex, 2, 2'- [-1, 2-cyclohexanediylbis(nitriloethylidyne)] bis(4-chlorophenol) (CNCP), against alcohol-induced gastric lesion in SD rats was assessed. SD rats were divided into four groups, i.e. normal, ulcer control, testing, and reference groups. Ulcer area, gastric wall mucus, and also gastric acidity of the animal sto...
Source: Acta Biochimica et Biophysica Sinica - December 30, 2019 Category: Biochemistry Authors: Saremi K, Rad SK, Khalilzadeh M, Hussaini J, Majid NA Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

PKM2-dependent glycolysis promotes the proliferation and migration of vascular smooth muscle cells during atherosclerosis.
Abstract Increased glycolysis is involved in the proliferation and migration of vascular smooth muscle cells (VSMCs). Pyruvate kinase isoform M2 (PKM2), a key rate-limiting enzyme in glycolysis, accelerates the proliferation and migration of tumor cells. Although the intracellular mechanisms associated with oxidized low-density lipoprotein (oxLDL)-stimulated VSMC proliferation and migration have been extensively explored, it is still unclear whether oxLDL promotes the proliferation and migration of VSMCs by enhancing PKM2-dependent glycolysis. In the present study, we detected PKM2 expression and pyruvate kinase a...
Source: Acta Biochimica et Biophysica Sinica - December 23, 2019 Category: Biochemistry Authors: Zhao X, Tan F, Cao X, Cao Z, Li B, Shen Z, Tian Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Regulatory mechanisms of endometrial decidualization and pregnancy-related diseases.
Abstract Endometrial decidualization is one of the earliest changes by which the uterus adapts to pregnancy. During this period, the endometrium undergoes complex changes in its biochemistry, physiology, and function at various levels, providing a suitable microenvironment for embryo implantation and development. Favorable decidualization lays an essential foundation for subsequent gestation, without which pregnancy failure or pregnancy complications may occur. The interaction between pregnancy-related hormones and cytokines produced by embryonic and uterine cells is known to be essential for decidualization, in w...
Source: Acta Biochimica et Biophysica Sinica - December 17, 2019 Category: Biochemistry Authors: Sang Y, Li Y, Xu L, Li D, Du M Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Resveratrol attenuates diabetes-associated cell centrosome amplification via inhibiting the PKC α-p38 to c-myc/c-jun pathway.
In conclusion, our results suggest that PKCα-p38 to c-myc/c-jun is the signaling pathway of the diabetes-associated centrosome amplification, and resveratrol attenuates the centrosome amplification by inhibiting this signaling pathway. PMID: 31844893 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - December 16, 2019 Category: Biochemistry Authors: Wu Q, Chen X, He Q, Lang L, Xu P, Wang P, Lee SC Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Losartan protects against intermittent hypoxia-induced peritubular capillary loss by modulating the renal renin-angiotensin system and angiogenesis factors.
Abstract Obstructive sleep apnea is characterized by chronic intermittent hypoxia (CIH), which is a risk factor for renal peritubular capillary (PTC) loss, and angiotensin II receptor blockers can alleviate PTC loss. However, the mechanism by which losartan (an angiotensin II receptor blocker) reduces CIH-induced PTC loss and attenuates kidney damage is still unknown. Thus, in this study, we examined the protective effects of losartan against CIH-induced PTC loss and explored the underlying mechanisms in rat CIH model. The immunohistochemical staining of CD34 and morphological examination showed that CIH reduced P...
Source: Acta Biochimica et Biophysica Sinica - December 14, 2019 Category: Biochemistry Authors: Wu J, Chu Y, Jiang Z, Yu Q Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Three putative DNA replication/repair elements encoding genes confer self-resistance to distamycin in Streptomyces netropsis.
Abstract Distamycin (DST) is a well-characterized DNA minor groove binder with antivirus activity and antitumor potency. Two separate gene clusters (a 28-kb cluster and a 7-kb cluster) have recently been identified to coordinately encode the biosynthetic machinery of DST in Streptomyces netropsis. Here we report a gene cassette, which is linked to the aforementioned smaller dst gene cluster and plays an important role in the self-resistance to DST in S. netropsis. This cassette consists of three uncharacterized genes that might be implicated in DNA replication/repair. Knockout of the cassette led to the decrease i...
Source: Acta Biochimica et Biophysica Sinica - December 12, 2019 Category: Biochemistry Authors: Ma L, Sun S, Yuan Z, Deng Z, Tang Y, Yu Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Antidotal effects of calcium folinate against high-dose methotrexate-induced damages to mouse matured oocytes.
PMID: 31833536 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - December 12, 2019 Category: Biochemistry Authors: Tian N, Ma W Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

MiR-122-5p suppresses the proliferation, migration, and invasion of gastric cancer cells by targeting LYN.
In this study, we found that miR-122-5p was low-expressed in GC tissues and cell lines by using qRT-PCR. Overexpression of miR-122-5p inhibited the proliferation, migration, and invasion of GC cells by using CCK-8 and transwell assays. On the contrary, downregulation of miR-122-5p promoted the proliferation, migration, and invasion of GC cells. In addition, we found that the expression of LYN, an Src family tyrosine kinase, was inversely correlated with miR-122-5p expression in GC tissues by using western blot analysis, immunohistochemistry, and qRT-PCR assays. Meanwhile, luciferase assay results indicated that LYN is a di...
Source: Acta Biochimica et Biophysica Sinica - December 11, 2019 Category: Biochemistry Authors: Meng L, Chen Z, Jiang Z, Huang T, Hu J, Luo P, Zhang H, Huang M, Huang L, Chen Y, Lu M, Xu AM, Ying S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

CAF-induced placental growth factor facilitates neoangiogenesis in hepatocellular carcinoma.
Abstract As a highly malignant tumor, hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. In most HCC patients, the development of HCC begins with hepatitis, which is followed by fibrosis and cirrhosis before progressing to HCC. Cancer-associated fibroblasts (CAFs), which are generally believed to be derived from activated hepatic stellate cells (HSCs), are highly involved in the development of HCC through the secretion of cytokines and angiogenic factors. The results of our study showed that a considerable number of CAFs highly expressed CD90 and were enriched in HCC tiss...
Source: Acta Biochimica et Biophysica Sinica - December 11, 2019 Category: Biochemistry Authors: Liu Z, Chen M, Zhao R, Huang Y, Liu F, Li B, Qin Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Loss of the third C2H2 zinc finger of chicken KLF7 affects its transcriptional regulation activities in adipose tissue.
Abstract KLF7, one of candidate genes in neurotherapy and metabolic syndrome, has been studied in adipogenesis of mammalian species and birds. However, the effect of the third C2H2 zinc finger of KLF7 for its transcriptional regulation in adipogenesis has not been well understood. Here, the wild-type chicken KLF7 (KLF7) overexpression plasmid, pCMV-myc-KLF7, and two plasmids of chicken KLF7 mutants, i.e. pCMV-myc-KLF7m1 with half of the third zinc finger (KLF7m1) and pCMV-myc-KLF7m2 without the third zinc finger (KLF7m2), were constructed. Luciferase reporter assay in DF1 cells showed that the effect of chicken KL...
Source: Acta Biochimica et Biophysica Sinica - December 11, 2019 Category: Biochemistry Authors: Zhang Z, Wu C, Lin T, Chen Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Delphinidin-induced autophagy protects pancreatic β cells against apoptosis resulting from high-glucose stress via AMPK signaling pathway.
In conclusion, our data demonstrate that delphinidin protects pancreatic β cells against high-glucose-induced injury by autophagy regulation via the AMPK signaling pathway. These findings might shed light on the underlying mechanisms of diabetes and help improve the prevention and therapy of this common disease. PMID: 31781740 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - November 29, 2019 Category: Biochemistry Authors: Lai D, Huang M, Zhao L, Tian Y, Li Y, Liu D, Wu Y, Deng F Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Quercetin improves endothelial insulin sensitivity in obese mice by inhibiting Drp1 phosphorylation at serine 616 and mitochondrial fragmentation.
Abstract Studies have shown that endothelial insulin resistance induced by oxidative stress contributes to vascular dysfunction in metabolic disorders. Quercetin, a natural antioxidant, has been recently shown to exert protective effects on endothelial function. However, the effects of quercetin on endothelial insulin resistance and its underlying mechanism are unclear. Here, we found that chronic oral treatment of obese mice with quercetin increased vascular endothelial insulin sensitivity, accompanied by alleviated mitochondrial fragmentation as revealed by confocal imaging. In addition, western blot analysis sh...
Source: Acta Biochimica et Biophysica Sinica - November 28, 2019 Category: Biochemistry Authors: Chen C, Huang J, Shen J, Bai Q Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

The expression changes of circular RNAs between LH  + 2 and LH + 7 human endometrium.
The expression changes of circular RNAs between LH + 2 and LH + 7 human endometrium. Acta Biochim Biophys Sin (Shanghai). 2019 Nov 28;: Authors: Hu S, Wang Y, Yao G, Sun Y PMID: 31774906 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - November 28, 2019 Category: Biochemistry Authors: Hu S, Wang Y, Yao G, Sun Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Expression of Nup93 is associated with the proliferation, migration and invasion capacity of cervical cancer cells.
In this study, the expression of Nup93 was analyzed by quantitative real-time polymerase chain reaction (qPCR), western blot analysis, and immunohistochemical staining in cervical cancer tissues and cell lines. We found that the expression of Nup93 was higher in cervical cancer samples, compared to normal cervical samples. The knockdown of Nup93 inhibited cell proliferation, migration, and invasion capacity of cervical cancer cells. At the same time, we also found that silencing of Nup93 could inhibit cellular migration and invasion by regulating cytoskeleton actin and Rho family proteins. Nup93 also participated in the IL...
Source: Acta Biochimica et Biophysica Sinica - November 28, 2019 Category: Biochemistry Authors: Ouyang X, Hao X, Liu S, Hu J, Hu L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

A novel protein L-cofilin2 identified in Lampetra morii with roles of immune response and cell proliferation promotion.
PMID: 31774909 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - November 28, 2019 Category: Biochemistry Authors: Li Y, Zuo Y, Zhang W, Zhao Y, Li Q Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Decreased expression of piR-35413 in human papillary thyroid cancer.
PMID: 31774911 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - November 28, 2019 Category: Biochemistry Authors: Liu L, Ren L, Shen L, Zhang C, Zhu H, Gu M, Li X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

SRC3 expressed in bone marrow mesenchymal stem cells promotes the development of multiple myeloma.
Abstract SRC3 plays critical roles in various biological processes of diseases, including proliferation, apoptosis, migration, and cell cycle arrest. However, the effect of SRC3 expression in mesenchymal stem cells (MSCs) on multiple myeloma (MM) is not clear yet. In our study, MSCs (MSC-SRC3, MSC-SRC3-/-) and MM cells were co-cultured in a direct or indirect way. The proliferation of MM cells was studied by CCK-8 and colony formation assays. The apoptosis and cell cycle of MM cells were detected by flow cytometry. In addition, the expressions of proteins in MM cells were detected by western blot analysis and the ...
Source: Acta Biochimica et Biophysica Sinica - November 26, 2019 Category: Biochemistry Authors: Jin J, Cheng S, Wang Y, Wang T, Zeng D, Li Z, Li X, Wang J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Exosome-encapsulated miR-505 from ox-LDL-treated vascular endothelial cells aggravates atherosclerosis by inducing NET formation.
This study aims to evaluate whether exosomes from oxidized low-density lipoprotein (ox-LDL)-treated vascular endothelial cells (VECs) aggravate AS by inducing NET formation. Exosomes from the peripheral blood of healthy donors and AS patients (namely NC-EXO and AS-EXO, respectively) and exosomes from human umbilical vein endothelial cells (HUVECs) treated without or with ox-LDL (namely normal EXO and ox-LDL-EXO, respectively) were isolated, identified, and co-cultured with neutrophils from peripheral blood of healthy donors. NET formation was evaluated by immunofluorescence staining and determining the content of cell-free...
Source: Acta Biochimica et Biophysica Sinica - November 25, 2019 Category: Biochemistry Authors: Chen L, Hu L, Li Q, Ma J, Li H Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

In vitro leishmanicidal activity of antimicrobial peptide KDEL against Leishmania tarentolae.
Abstract Leishmaniasis, caused by the intracellular protozoan parasite Leishmania, remains an important neglected tropical infectious disease. Infection may be lethal if untreated. Currently, the available drugs for the disease are limited by high toxicity and drug resistance. There is an urgent need to develop novel anti-leishmanial strategies. Antimicrobial peptides (AMPs) have been described as the first-line immune defense against pathogenic microbes and are being developed as emerging anti-parasitic therapies. In the present study, we showed the anti-leishmanial activity of the synthetic 4-amino acid peptide ...
Source: Acta Biochimica et Biophysica Sinica - November 22, 2019 Category: Biochemistry Authors: Cao L, Jiang W, Cao S, Zhao P, Liu J, Dong H, Guo Y, Liu Q, Gong P Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Syringic acid promotes proliferation and migration of Schwann cells via down-regulating miR-451-5p.
In this study, we for the first time found that SA was able to promote the proliferation and migration of Schwann cells, two important abilities in the process of regeneration. Then, microRNA (miRNA) microarray analysis was performed and 26 differentially expressed miRNAs (22 down-regulated and 4 up-regulated) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway analyses found that the target genes of these miRNAs were mainly enriched in cellular response to chemical stimulus and cancer-related pathways, respectively. Subsequently, the levels of top 6 down-regulated miRNA...
Source: Acta Biochimica et Biophysica Sinica - November 21, 2019 Category: Biochemistry Authors: Lin Y, Jiang X, Yin G, Lin H Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Disruption of crosstalk between LX-2 and liver cancer stem-like cells from MHCC97H cells by DFOG via inhibiting FOXM1.
This study demonstrated that DFOG disrupts the crosstalk between HSCs and LCSLCs to suppress LCSLC features via down-regulating FOXM1 expression and reducing HGF secretion in HSCs. PMID: 31750892 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - November 21, 2019 Category: Biochemistry Authors: Chen A, Xu C, Luo Y, Liu L, Song K, Deng G, Yang M, Cao J, Yuan L, Li X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

LncRNA TUG1 mediates lipopolysaccharide-induced proliferative inhibition and apoptosis of human periodontal ligament cells by sponging miR-132.
In this study, we explored the functions of TUG1 and its underlying mechanisms in the inflammatory process induced by Porphyromonas gingivalis-derived lipopolysaccharide (LPS) in PDLCs. Our results showed that TUG1 had a decreased expression in both periodontal ligament (PDL) tissues with periodontitis and PDLCs under a LPS-induced inflammatory condition, and TUG1 expression was negatively correlated with miR-132 expression in periodontitis-affected PDL tissues. Furthermore, we found that TUG1 overexpression in PDLCs alleviated LPS-induced proliferative inhibition and apoptosis promotion, while TUG1 knockdown had the oppos...
Source: Acta Biochimica et Biophysica Sinica - November 18, 2019 Category: Biochemistry Authors: Han Y, Wang F, Shao L, Huang P, Xu Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Biophysical characterization of oligomerization and fibrillization of the G131V pathogenic mutant of human prion protein.
Abstract The pathogenesis of fatal neurodegenerative prion diseases is closely associated with the conversion of α-helix-rich cellular prion protein into β-sheet-rich scrapie form. Pathogenic point mutations of prion proteins usually promote the conformational conversion and trigger inherited prion diseases. The G131V mutation of human prion protein (HuPrP) was identified to be involved in Gerstmann-Sträussler-Scheinker syndrome. Few studies have been carried out to address the pathogenesis of the G131V mutant. Here, we addressed the effects of the G131V mutation on oligomerization and fibrillizati...
Source: Acta Biochimica et Biophysica Sinica - November 18, 2019 Category: Biochemistry Authors: Zhang M, Zhang H, Yao H, Guo C, Lin D Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Molecular hydrogen: current knowledge on mechanism in alleviating free radical damage and diseases.
Abstract Ever since molecular hydrogen was first reported as a hydroxyl radical scavenger in 2007, the beneficial effect of hydrogen was documented in more than 170 disease models and human diseases including ischemia/reperfusion injury, metabolic syndrome, inflammation, and cancer. All these pathological damages are concomitant with overproduction of reactive oxygen species (ROS) where molecular hydrogen has been widely demonstrated as a selective antioxidant. Although it is difficult to construe the molecular mechanism of hydrogen's biomedical effect, an increasing number of studies have been helping us draw the...
Source: Acta Biochimica et Biophysica Sinica - November 18, 2019 Category: Biochemistry Authors: Tao G, Song G, Qin S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Hypothalamic paraventricular nucleus neurons activated by estrogen GPER1 receptors promote anti-inflammation effects in the early stage of colitis.
In this study, we used the dextran sulfate sodium (DSS)-induced mice colitis model, gene editing, and RNA interference to determine the effects of PVN neurons on intestinal inflammation. We found that at the early stage (third day) after DSS treatment, there was a mild inflammation in the colorectal area and an increased neuron activation in the PVN but not in the adjacent area. At the same time, ~80% of activated PVN neurons also expressed novel estrogen GPER1 receptor. The colitis noticeably worsened in GPER1-knockout mice and local PVN GPER1-knockdown mice. These results indicated that PVN GPER1 positive neurons potenti...
Source: Acta Biochimica et Biophysica Sinica - November 15, 2019 Category: Biochemistry Authors: Jiang T, Wang R, Yin W, Zhou Y, Kong D, Xu S, Gao P, Yu W, Jiao Y, Wen D Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

FBXL19-AS1 promotes cell proliferation and inhibits cell apoptosis via miR-876-5p/FOXM1 axis in breast cancer.
In this study, we set out to find more reliable downstream molecules of FBXL19-AS1 in breast cancer. FBXL19-AS1 was expressed at a high level in breast cancer cells. Loss-of-function experiments revealed that silencing FBXL19-AS1 could impair cell proliferation and induce cell apoptosis in breast cancer. In addition, the location of FBXL19-AS1 in the cytoplasm was detected by fluorescent in situ hybridization assay, while FBXL19-AS1 regulated the expression of Forkhead box M1 (FOXM1) by directly absorbing miR-876-5p. Through rescue assays, it was observed that FOXM1 overexpression recovered the inhibited tumor growth cause...
Source: Acta Biochimica et Biophysica Sinica - November 6, 2019 Category: Biochemistry Authors: Dong G, Pan T, Zhou D, Li C, Liu J, Zhang J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Wogonoside inhibits tumor growth and metastasis in endometrial cancer via ER stress-Hippo signaling axis.
In this study, we explored the function of wogonoside on tumor growth and the underlying mechanism on endometrial cancer. Firstly, we investigated the effect of wogonoside on endometrial cancer cells and found that wogonoside could significantly decrease cell proliferation and metastasis. Mechanistically, wogonoside could aggravate the extent of ER stress and upregulate the phosphorylation level of Mammalian Ste20-like kinase 1, leading to the activation of the Hippo signaling pathway. Taken together, in vitro and in vivo data demonstrated that wogonoside could be a potent inducer of ER stress and could be further develope...
Source: Acta Biochimica et Biophysica Sinica - November 6, 2019 Category: Biochemistry Authors: Chen S, Wu Z, Ke Y, Shu P, Chen C, Lin R, Shi Q Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Mitochondrial permeability with autophagy in lifespan shortening: a novel therapeutic target for aging.
PMID: 31680147 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - November 4, 2019 Category: Biochemistry Authors: Yao H, Zhang Y, Zeng Z, Zhou H Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

UBE3A alleviates isoproterenol-induced cardiac hypertrophy through the inhibition of the TLR4/MMP-9 signaling pathway.
In this study, we explored the potential mechanism underlying cardiac hypertrophy induced by isoproterenol. Our results showed that isoproterenol induced cardiac hypertrophy in AC16 cells, as reflected by the increased cell surface area and increased hypertrophic markers, which was accompanied by increased ubiquitin-protein ligase E3a (UBE3A) expression. Moreover, UBE3A knockdown by siRNAs accelerated cardiac hypertrophy, suggesting that increased UBE3A expression induced by isoproterenol might be a protective response and UBE3A might be a protective factor against cardiac hypertrophy. Our study also revealed that UBE3A kn...
Source: Acta Biochimica et Biophysica Sinica - November 4, 2019 Category: Biochemistry Authors: Li Y, Ma L, Gu S, Tian J, Cao Y, Jin Z, Chen J, Gu B, Tu J, Wang Z, Li X, Ning Z, Jin Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Recombinant expression, biophysical and functional characterization of ClpS from Mycobacterium tuberculosis.
Abstract Intracellular proteolysis is attracting more and more attention for its unique and important character in Mycobacterium tuberculosis (Mt). The ClpS protein from Mt (MtClpS) plays a critical role in intracellular proteolysis by recognizing N-end rule substrates, which makes it become a potential target for antibacterial drugs. However, the molecular mechanism of MtClpS recognizing N-end rule substrates remains unclear. Preparation of highly concentrated and pure MtClpS protein is a prerequisite for further structural and functional studies. In the present work, we tried several fusion tags and various expr...
Source: Acta Biochimica et Biophysica Sinica - October 25, 2019 Category: Biochemistry Authors: Guo C, Xiao Y, Bi F, Lin W, Wang H, Yao H, Lin D Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Beclin1-mediated ferroptosis activation is associated with isoflurane-induced toxicity in SH-SY5Y neuroblastoma cells.
Abstract The widely used inhalation anesthetic, isoflurane, potentially induces neuronal injury in clinical practice. Previous studies showed multiple forms of cell death that resulted from isoflurane-induced cytotoxicity, but the precise underlying mechanism remains poorly understood. Ferroptosis has recently been identified as a non-apoptotic form of regulated cell death. Here, we found that ferroptosis inhibitors, ferrostatin-1 and deferoxamine mesylate (DFOM), showed great efficiency in maintaining cell viability in SH-SY5Y neuroblastoma cells exposed to a high concentration of isoflurane for 24 h. We als...
Source: Acta Biochimica et Biophysica Sinica - October 24, 2019 Category: Biochemistry Authors: Liu R, Li X, Zhao G Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Downregulation of MANCR inhibits cancer cell proliferation in mantle cell lymphoma possibly by interacting with RUNX2.
In this study, we therefore studied the role of MANCR in mantle cell lymphoma (MCL). We found that serum MANCR and Runt-related transcription factor 2 (RUNX2) were upregulated in MCL patients when compared with those in healthy controls. A positive correlation between serum MANCR and RUNX2 was found in MCL patients but not in controls. Upregulation of serum MANCR distinguished MCL patients from controls. MANCR overexpression promoted RUNX2 expression in MCL cells, while RUNX2 overexpression failed to significantly change the expression levels of MANCR. MANCR overexpression promoted the proliferation of MCL cells, while MAN...
Source: Acta Biochimica et Biophysica Sinica - October 24, 2019 Category: Biochemistry Authors: Wen S, Zeng M, Li Y, Hu X, Li S, Liang X, Zhu L, Yang S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Propofol attenuates the adhesion of tumor and endothelial cells through inhibiting glycolysis in human umbilical vein endothelial cells.
Abstract Propofol is one of the most commonly used intravenous anesthetics and plays an important role in tumor suppression. In the present study, we aimed to investigate the mechanism by which propofol attenuates tumor endothelial cells (TECs) and tumor cell adhesion to inhibit tumor metastasis in vitro. Human umbilical vein endothelial cells (HUVECs) cultured in Dulbecco's modified Eagle's medium were treated with tumor conditioned medium for 24 h, followed by 4 h of treatment with or without 25 μM of propofol, 10 μM of KN93, 500 μM of MK801, or 20 μM of rapastinel. It was found that propofol inhibit...
Source: Acta Biochimica et Biophysica Sinica - October 24, 2019 Category: Biochemistry Authors: Qi J, Wu Q, Zhu X, Zhang S, Chen X, Chen W, Sun Z, Zhu M, Miao C Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

LncRNA-MALAT1 mediates cisplatin resistance via miR-101-3p/VEGF-C pathway in bladder cancer.
In this study, we firstly demonstrated that MALAT1 expression was up-regulated in the BC tissues compared to the normal adjacent tissues and elevated in the cancer cells compared to the epithelial immortalized cells. Secondly, we found that suppression of MALAT1 enhanced the chemotherapeutic drug sensitivity and inhibited the cisplatin resistance of the BC cells. Thirdly, we showed that MALAT1 affected the cisplatin resistance of the BC cells via regulating the miR-101-3p/VEGF-C pathway. In summary, this study demonstrates that MALAT1, miR-101-3p and VEGF-C form a regulatory axis to affect the chemo-resistance of BC cells ...
Source: Acta Biochimica et Biophysica Sinica - October 23, 2019 Category: Biochemistry Authors: Liu P, Li X, Cui Y, Chen J, Li C, Li Q, Li H, Zhang X, Zu X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Interactive effect of BCL2 and BAX polymorphisms with cigarette smoking on the risk of non-small cell lung cancer: a case-control study in China.
PMID: 31642881 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - October 23, 2019 Category: Biochemistry Authors: Jiang P, Tang J, Shao Q, Cai Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

CmRAV1 shows differential expression in two melon (Cucumis melo L.) cultivars and enhances salt tolerance in transgenic Arabidopsis plants.
This study demonstrated that the expression of CmRAV1 was associated with saline stress and can potentially be utilized to improve plant salt tolerance. PMID: 31620769 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - October 17, 2019 Category: Biochemistry Authors: Zhao L, Zhang F, Liu B, Yang S, Xiong X, Hassani D, Zhang Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research