Characteristics of lipid metabolism-related gene expression-based molecular subtype in papillary thyroid cancer.
PMID: 32880619 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - September 3, 2020 Category: Biochemistry Authors: Xu M, Sun T, Wen S, Zhang T, Wang X, Cao Y, Wang Y, Sun X, Ji Q, Shi R, Qu N Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

A novel mannose-binding lectin from Liparis nervosa with anti-fungal and anti-tumor activities.
Abstract Plant lectins are carbohydrate-binding proteins with nonimmune origin, which can reversibly bind with carbohydrates, agglutinate cells, and precipitate polysaccharides and glycoconjugates. Plant lectins have attracted much attention for their anti-virus, anti-proliferation, and pro-apoptosis properties. Thus the exploration of new lectins has received special attention. Here we purified a mannose-binding lectin from the rhizomes of Liparis nervosa by ion exchange chromatography on DEAE-Sepharose, affinity chromatography on Mannose-Sepharose 4B, and gel filtration chromatography on Sephacryl S-100. The pur...
Source: Acta Biochimica et Biophysica Sinica - August 27, 2020 Category: Biochemistry Authors: Jiang N, Wang Y, Zhou J, Zheng R, Yuan X, Wu M, Bao J, Wu C Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

NEAT1/miR-140-3p/MAPK1 mediates the viability and survival of coronary endothelial cells and affects coronary atherosclerotic heart disease.
In this study, we aimed to investigate the functions of lncRNA NEAT1 in CAD. RT-PCR and western blot analysis were carried out to examine the expressions of related RNAs. Colony formation assay, cell proliferation assay, apoptosis assay, and dual-luciferase reporter assay were conducted to investigate the abilities of colony migration, cell proliferation, apoptosis, and targeting. The results showed that NEAT1 was up-regulated in CAD blood samples and in human coronary endothelial cells (HCAECs). Transfection of pcNEAT1 significantly inhibited the survival rate of HCAECs and induced apoptosis of HCAECs. MiR-140-3p was down...
Source: Acta Biochimica et Biophysica Sinica - August 26, 2020 Category: Biochemistry Authors: Zhang H, Ji N, Gong X, Ni S, Wang Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Comparison of the differentiation of dental pulp stem cells and periodontal ligament stem cells into neuron-like cells and their effects on focal cerebral ischemia.
In this study, the in vitro differentiation of DPSCs and PDLSCs into neuron-like cells was evaluated. Then, we established a rat model of cerebral ischemia. DPSCs or PDLSCs were administered to animals, and the therapeutic effects of these two types of cells were investigated. The results showed that PDLSCs had a higher differentiation rate than DPSCs. Immunofluorescence studies showed that the expression of the neuronal differentiation marker Thy-1 was higher in PDLSCs than in DPSCs, and other gene markers of neuronal differentiation showed corresponding trends, which were confirmed by western blot analysis. In this proce...
Source: Acta Biochimica et Biophysica Sinica - August 26, 2020 Category: Biochemistry Authors: Wu T, Xu W, Chen H, Li S, Dou R, Shen H, Liu X, Liu X, Hong Y, He J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Quercetin promotes human epidermal stem cell proliferation through the estrogen receptor/ β-catenin/c-Myc/cyclin A2 signaling pathway.
In this study, we investigated the effect of quercetin on human EpSC proliferation and explored the underlying mechanisms. We found that quercetin at 0.1~1 μM significantly promoted EpSC proliferation and increased the number of cells in S phase. The pro-proliferative effect of quercetin on EpSCs was confirmed in cultured human skin tissue. Mechanistic studies showed that quercetin significantly upregulated the expressions of β-catenin, c-Myc, and cyclins A2 and E1. Inhibitor for β-catenin or c-Myc significantly inhibited quercetin-induced EpSC proliferation. The β-catenin inhibitor XAV-939 suppresse...
Source: Acta Biochimica et Biophysica Sinica - August 25, 2020 Category: Biochemistry Authors: Wang Z, Zhang G, Le Y, Ju J, Zhang P, Wan D, Zhao Q, Jin G, Su H, Liu J, Feng J, Fu Y, Hou R Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

IRP2-Hif1 α/Hif2α signaling: a novel mechanism of metabolic switch from aerobic glycolysis to oxidative phosphorylation.
IRP2-Hif1α/Hif2α signaling: a novel mechanism of metabolic switch from aerobic glycolysis to oxidative phosphorylation. Acta Biochim Biophys Sin (Shanghai). 2020 Aug 19;: Authors: Zhu L, Zhou Q, He L, Chen L PMID: 32813008 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - August 19, 2020 Category: Biochemistry Authors: Zhu L, Zhou Q, He L, Chen L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Abnormal expression of homeobox c6 in the atherosclerotic aorta and its effect on proliferation and migration of rat vascular smooth muscle cells.
Abstract Homeobox c6 (Hoxc6) affects the proliferation, migration, and infiltration of malignant tumor cells; however, the effect of Hoxc6 on atherosclerosis (AS) as well as the proliferation and migration of vascular smooth muscle cells (VSMCs), which play a role in promoting AS, has not yet been well clarified. In the present study, we tested the hypothesis that Hoxc6 affects the proliferation and migration of rat VSMCs, and hence is involved in AS. The results showed that the expression of Hoxc6 mRNA and protein was higher in normal rat aortic wall than in the myocardium. Subsequently, a rat model of AS was est...
Source: Acta Biochimica et Biophysica Sinica - August 12, 2020 Category: Biochemistry Authors: Long X, You G, Wu Q, Zhou Y, Yu F, Xiao Y, Deng S, Song F, Huang J, Tian M Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Mitochondrial metabolism in regulating macrophage polarization: an emerging regulator of metabolic inflammatory diseases.
Yin K Abstract As a major type of immune cells with heterogeneity and plasticity, macrophages are classically divided into inflammatory (M1) and alternative/anti-inflammatory (M2) types and play a crucial role in the progress of the inflammatory diseases. Recent studies have shown that metabolism is an important determinant of macrophage phenotype. Mitochondria, one of the most important compartments involving cell metabolism, are closely associated with the regulation of cell functions. In most types of cell, mitochondrial oxidative phosphorylation (OXPHOS) is the primary mode of cellular energy production. Howev...
Source: Acta Biochimica et Biophysica Sinica - August 12, 2020 Category: Biochemistry Authors: Qing J, Zhang Z, Novák P, Zhao G, Yin K Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Pannexin-2, a novel mitochondrial-associated membrane protein, may become the new strategy to treat and prevent neurological disorders.
PMID: 32770209 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - August 8, 2020 Category: Biochemistry Authors: Yang Y, Wang L, Chen L, Li L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Guanine nucleotide-binding protein subunit beta-4 promotes gastric cancer progression via activating Erk1/2.
In this study, we found that GNB4 was highly expressed in GC tissues compared to that in non-tumor tissues and was significantly associated with tumor invasion depth, pathological stage and poor survival rate of GC patients. Both gain-of-function and loss-of-function studies revealed that GNB4 significantly enhanced GC cell growth and motility both in vitro and in vivo. Further studies revealed that GNB4 overexpression induced G1-S transition and promoted the process of epithelial-mesenchymal transformation. These tumor promoting effects were mediated by GNB4 which activates the Erk1/2 pathway through upregulating Erk1/2 p...
Source: Acta Biochimica et Biophysica Sinica - August 4, 2020 Category: Biochemistry Authors: Gao J, Pan H, Zhu Z, Yu T, Huang B, Zhou Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Identification of host protein CBL interacting with Eimeria acervulina microneme protein MIC3.
PMID: 32743636 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - July 31, 2020 Category: Biochemistry Authors: Wang P, Zhao H, Wang Q, Gao C, Wu H, Cheng C, Song H Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Identification of novel miRNAs in mouse embryonic stem cells and reprogrammed pluripotent cells.
PMID: 32734301 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - July 30, 2020 Category: Biochemistry Authors: Zhao B, Fan C, Jin Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Comprehensive analysis of circular RNA expression profiles in cisplatin-resistant non-small cell lung cancer cell lines.
Abstract Platinum-based drugs such as cisplatin are widely used in combination chemotherapy for non-small cell lung cancer (NSCLC) owing to their high clinical response rate; however, acquired resistance to cisplatin is eventually inevitable. Circular RNAs (circRNAs) are involved in the development of diverse types of cancers, but their connection to cisplatin-resistance in NSCLC has not been studied. In the present study, two cisplatin-resistant NSCLC cell lines (A549/DDP and PC9/DDP) were established by gradually increasing concentrations of cisplatin in the media. The resulting cell lines possessed high resista...
Source: Acta Biochimica et Biophysica Sinica - July 27, 2020 Category: Biochemistry Authors: Song L, Cui Z, Guo X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Baicalein suppresses vasculogenic mimicry through inhibiting RhoA/ROCK expression in lung cancer A549 cell line.
In this study, baicalein was found to inhibit the viability and motility of A549 cells and induced the breakage of the cytoskeletal actin filament network. In addition, baicalein significantly decreased the formation of VM and downregulated the expressions of VM-associated factors, such as VE-cadherin, EphA2, MMP14, MMP2, MMP9, PI3K and LAMC2, similar to the effects of ROCK inhibitors. Indeed, baicalein inhibited RhoA/ROCK expression in vitro and in vivo, suggesting the underlying mechanisms of reduced VM formation. Collectively, baicalein suppressed the formation of VM in NSCLC by targeting the RhoA/ROCK signaling pathway...
Source: Acta Biochimica et Biophysica Sinica - July 16, 2020 Category: Biochemistry Authors: Zhang Z, Nong L, Chen M, Gu X, Zhao W, Liu M, Cheng W Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

JNK inhibition alleviates oxidative DNA damage, germ cell apoptosis, and mitochondrial dysfunction in testicular ischemia reperfusion injury.
In conclusion, JNK signaling regulates oxidative DNA damage, GCA, and mitochondrial dysfunction through activation of p53 and suppression of survivin during tIRI. PMID: 32662511 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - July 14, 2020 Category: Biochemistry Authors: Fadel F, Al-Kandari N, Khashab F, Al-Saleh F, Al-Maghrebi M Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Corrigendum to: Gene expression profiling of cells of origin of squamous cell carcinomas in head-and-neck, esophagus, and lung.
PMID: 32643758 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - July 9, 2020 Category: Biochemistry Authors: Qu N, Huang D, Xu Q, Wang J, Chen C, Ji Q, Wu W, Zhu Y, Xu M Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

miR-28-5p targets MTSS1 to regulate cell proliferation and apoptosis in esophageal cancer.
In this study, qRT-PCR and western blot analysis revealed that miR-28-5p expression was up-regulated and metastasis suppressor-1 (MTSS1) was down-regulated in EC tissues relative to matched para-cancer tissues. Cell counting kit-8 (CCK-8) assay demonstrated that miR-28-5p mimics increased cell viability, and miR-28-5p inhibitor decreased it. Flow cytometry (FCM) assay indicated that miR-28-5p mimics promoted cell cycle entry, while miR-28-5p inhibitor reduced it and induced cell apoptosis. Moreover, miR-28-5p mimics up-regulated the expressions of cyclin A, cyclin dependent kinase 2 (CDK2), cyclin D1, and cyclin E but down...
Source: Acta Biochimica et Biophysica Sinica - July 9, 2020 Category: Biochemistry Authors: Zhang L, Wang X, Liu X, Lv M, Shen E, Zhu G, Sun Z Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

β -Cypermethrin promotes the adipogenesis of 3T3-L1 cells via inducing autophagy and shaping an adipogenesis-friendly microenvironment.
In this study, mouse embryo fibroblasts 3T3-L1 cells were exposed to β-CYP, and the cell viability, intracellular reactive oxygen species (ROS) level, autophagy, and adipogenesis were assessed to investigate the roles of oxidative stress and autophagy in the toxic effects of β-CYP on adipogenesis. The results demonstrated that treatment with 100 μΜ β-CYP elevated the ROS level, decreased mitochondrion membrane potential, stimulated autophagy, and enhanced the adipogenesis induced by the mixture of insulin, dexamethasone, and 3-isobutyl-1-methylxanthine. However, co-treatment with N-acetyl-L-cystei...
Source: Acta Biochimica et Biophysica Sinica - July 8, 2020 Category: Biochemistry Authors: He B, Wang X, Jin X, Xue Z, Zhu J, Wang C, Jin Y, Fu Z Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Isoliquiritigenin inhibits TGF- β1-induced fibrogenesis through activating autophagy via PI3K/AKT/mTOR pathway in MRC-5 cells.
In this study, we aimed to explore the roles and the underlying mechanisms of ISL in TGF-β1-induced fibrogenesis using human lung fibroblast-derived MRC-5 cells. Cell proliferation and migration were determined by MTT and wound healing assay, respectively. The expression levels of alpha-smooth muscle actin (α-SMA), collagen type I alpha 1 (COLIA1) and fibronectin (FN), microtubule-associated protein light chain 3 (LC3) and related signaling molecules were detected by quantitative real-time PCR, western blot and immunofluorescence assay, correspondingly. EGFP-LC3 transfection was used for autophagy analysis. The ...
Source: Acta Biochimica et Biophysica Sinica - July 8, 2020 Category: Biochemistry Authors: He J, Peng H, Wang M, Liu Y, Guo X, Wang B, Dai L, Cheng X, Meng Z, Yuan L, Cai F, Tang Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Long non-coding RNA LINC-PINT attenuates paclitaxel resistance in triple-negative breast cancer cells via targeting the RNA-binding protein NONO.
Abstract The treatment of triple-negative breast cancer (TNBC) relies largely on chemotherapies. However, it is frequent that TNBC patients develop resistance to the chemotherapy drugs. Generation of drug-resistant cell lines facilitates the identification of drug resistance. Here, we established two paclitaxel (PTX)-resistant TNBC cancer cell lines using an intermittent and stepwise method and found that long non-coding RNA long intergenic non-protein-coding RNA p53-induced transcript (LINC-PINT) was significantly decreased in PTX-resistant cancer cells. Ectopic expression of LINC-PINT sensitized both PTX-resista...
Source: Acta Biochimica et Biophysica Sinica - July 7, 2020 Category: Biochemistry Authors: Chen J, Zhu M, Zou L, Xia J, Huang J, Deng Q, Xu R Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

ZnT8 in T2D: a novel therapeutic target for maintaining insulin secretion capacity.
PMID: 32634200 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - July 7, 2020 Category: Biochemistry Authors: Zhang Y, Jian W, Li D, He L, Wu J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Upregulation of miR-133a by adiponectin inhibits pyroptosis pathway and rescues acute aortic dissection.
In conclusion, our present results indicated that the upregulation of miR-133a by APN inhibits pyroptosis pathway, which potentially rescues AAD. PMID: 32634201 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - July 7, 2020 Category: Biochemistry Authors: Duan H, Zhang X, Song R, Liu T, Zhang Y, Yu A Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Cloning, characterization, and functional analysis of chitinase-like protein 1 in the shell of Pinctada fucata.
In this study, we first obtained the full-length cDNA sequence of Pf-Clp1 by RACE. Real-time polymerase chain reaction results revealed that Pf-Clp1 was highly expressed in the important biomineralization tissues, the mantle edge and the mantle pallial. We expressed and purified recombinant protein rPf-Clp1 in vitro to investigate the function of Pf-Clp1 on CaCO3 crystallization. Scanning electron microscopy imaging and Raman spectroscopy revealed that rPf-Clp1 was able to affect the morphologies of calcite crystal in vitro. Shell notching experiments suggested that Pf-Clp1 might function as a negative regulator during she...
Source: Acta Biochimica et Biophysica Sinica - July 7, 2020 Category: Biochemistry Authors: Zhou Y, Yan Y, Yang D, Zheng G, Xie L, Zhang R Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

HEATR1 promotes proliferation in gastric cancer in vitro and in vivo.
This study suggested that HEATR1 may be an oncogene and a target for GC therapy. PMID: 32634230 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - July 7, 2020 Category: Biochemistry Authors: Zhao J, Zhu Y, Fu Q, Zhu Y, Zhao G Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

miR-378 and its host gene Ppargc1 β exhibit independent expression in mouse skeletal muscle.
miR-378 and its host gene Ppargc1β exhibit independent expression in mouse skeletal muscle. Acta Biochim Biophys Sin (Shanghai). 2020 Jun 30;: Authors: Kang L, Han C, Yang G, Li H, Li T, Yang S, Liang N, Zhong R, Jia L, Zhu D, Zhang Y Abstract MicroRNAs (miRNAs) are implicated in multiple biological processes in physiological and pathological settings. Nearly half of the known miRNAs are classified as 'intronic' miRNAs because they are embedded within the introns of protein-coding or noncoding genes. Such miRNAs were thought to be processed from primary host gene transcripts and share the promote...
Source: Acta Biochimica et Biophysica Sinica - June 30, 2020 Category: Biochemistry Authors: Kang L, Han C, Yang G, Li H, Li T, Yang S, Liang N, Zhong R, Jia L, Zhu D, Zhang Y Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Strategies to improve the fluorescent signal of the tripartite sfGFP system.
Abstract Bimolecular fluorescence complementation (BiFC) is a popular method used to detect protein-protein interactions. For a BiFC assay, a fluorescent protein is usually split into two parts, and the fluorescence is recovered upon the interaction between the fused proteins of interest. As an elegant extension of BiFC, a tripartite superfold green fluorescent protein (sfGFP) system that has the advantages of low background fluorescence and small fusion tag size has been developed. However, the tripartite system exhibits a low fluorescence signal in some cases. To address this problem, we proposed to increase the...
Source: Acta Biochimica et Biophysica Sinica - June 24, 2020 Category: Biochemistry Authors: Shen J, Zhang W, Gan C, Wei X, Li J, Sun Y, Yuan Y, Cai X, Long Q, Cui J, Guo H, Huang A, Hu J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

The influence of rs139416141 in Neighbor of Brca1 gene (NBR1) on bone mineral density in postmenopausal Chinese women.
PMID: 32577726 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - June 23, 2020 Category: Biochemistry Authors: Zhang Y, Zhang Z, Zhang Z, Zhao L, Xue Y, Wu H, Hou J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Identification of potential key genes and key pathways related to clear cell renal cell carcinoma through bioinformatics analysis.
In this study, we conducted a bioinformatics analysis on three Gene Expression Omnibus datasets and identified 437 differentially expressed genes (DEGs) related to ccRCC development and prognosis, of which 311 and 126 genes are respectively down-regulated and up-regulated. The protein-protein interaction network of these DEGs consists of 395 nodes and 1872 interactions and 2 prominent modules. The Staphylococcus aureus infection and complement and coagulation cascades are significantly enriched in module 1 and are likely involved in ccRCC progression. Forty-two hub genes were screened, of which von Willebrand factor, TIMP ...
Source: Acta Biochimica et Biophysica Sinica - June 19, 2020 Category: Biochemistry Authors: Zhai W, Lu H, Dong S, Fang J, Yu Z Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

A special issue on the 10-year anniversary of Life Sciences Institute, Zhejiang University.
PMID: 32556237 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - June 19, 2020 Category: Biochemistry Authors: Feng XH Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

STAT3 and AKT signaling pathways mediate oncogenic role of NRSF in hepatocellular carcinoma.
Abstract Neuron-restrictive silencer factor (NRSF) is a zinc finger protein that acts as a negative transcriptional regulator by recruiting histone deacetylases and other co-factors. It plays a crucial role in nervous system development and is recently reported to be involved in tumorigenesis in a tumor type-dependent manner; however, the role of NRSF in hepatocellular carcinoma (HCC) tumorigenesis remains unclear. Here, we found that NRSF expression was up-regulated in 27 of 49 human HCC tissue samples examined. Additionally, mice with conditional NRSF-knockout in the liver exhibited a higher tolerance against di...
Source: Acta Biochimica et Biophysica Sinica - June 18, 2020 Category: Biochemistry Authors: Ma M, Zhou Y, Sun R, Shi J, Tan Y, Yang H, Zhang M, Shen R, Xu L, Wang Z, Fei J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Abemaciclib induces apoptosis in cardiomyocytes by activating the Hippo signaling pathway.
In this study, we used the cell counting kit-8 assay, western blot analysis, flow cytometry, immunostaining, and quantitative polymerase chain reaction to investigate the role of abemaciclib in inducing apoptosis and in inhibiting the viability and proliferation of AC16 human cardiomyocyte cells. The results revealed that abemaciclib induced apoptosis and inhibited cell proliferation by activating the Hippo signaling pathway. This work demonstrates the molecular basis by which abemaciclib induces cardiac side effects, providing a theoretical basis and effective targets for the treatment of cardiac diseases. PMID: 3255...
Source: Acta Biochimica et Biophysica Sinica - June 18, 2020 Category: Biochemistry Authors: Zhou Y, Li Y, Shen J, Li J, Li X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Inverted meiosis: an alternative way of chromosome segregation for reproduction.
Abstract Canonical meiosis is characterized by two sequential rounds of nuclear divisions following one round of DNA replication-reductional segregation of homologous chromosomes during the first division and equational segregation of sister chromatids during the second division. Meiosis in an inverted order of two nuclear divisions-inverted meiosis has been observed in several species with holocentromeres as an adaptive strategy to overcome the obstacle in executing a canonical meiosis due to the holocentric chromosome structure. Recent findings of co-existence of inverted and canonical meiosis in two monocentric...
Source: Acta Biochimica et Biophysica Sinica - June 16, 2020 Category: Biochemistry Authors: Li W, He X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Tudor domain of histone demethylase KDM4B is a reader of H4K20me3.
In this study, we purified the KDM4B chromatin-associated hybrid tudor domains (HTDs) and plant home domains (PHDs) and performed the pull-down assay to screen the tri-methyl modified histone peptides that could be efficiently recognized by KDM4B. Our results showed that both HTD alone and the combination of HTD and PHD were able to specifically bind to H3K4me3 and H4K20me3. Because H4K20me3 is essential for KDM4B's rapid recruitment to DNA damage site, we further aligned the multiple tudor peptide sequence and identified two conserved residues Y993 and W987 that are critical for KDM4B-H4K20me3 interaction. The surface pla...
Source: Acta Biochimica et Biophysica Sinica - June 15, 2020 Category: Biochemistry Authors: Xiang Y, Guo J, Li F, Xiong J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Corrigendum to: Ligands of peroxisome proliferator-activated receptor inhibit homocysteine induced DNA methylation of inducible nitric oxide synthase gene.
PMID: 32524139 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - June 11, 2020 Category: Biochemistry Authors: Jiang Y, Zhang J, Xiong J, Cao J, Li G, Wang S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Hippocampus is more vulnerable to neural damages induced by repeated sevoflurane exposure in the second trimester than other brain areas.
Abstract During the rapidly developing and sensitive period of the central nervous system (CNS), a harmful stimulus may have serious consequences. The effect of anesthetic exposure on the development of the offspring's CNS during pregnancy is still unclear and has been widely concerned. In the present study, we compared the susceptibility of the hippocampus with those of other brain regions in offsprings when the mother mice were exposed to repeated sevoflurane. We found that other than affecting motor sensation, emotion, or social behavior of offspring mice, repeated sevoflurane exposure induced significant memor...
Source: Acta Biochimica et Biophysica Sinica - June 9, 2020 Category: Biochemistry Authors: Chen B, Liu Y, Cai Y, Tang D, Xu S, Gao P, Yu W, Jiao Y, Li W Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Sinorhizobium meliloti NtrX interacts with different regions of the visN promoter.
PMID: 32510129 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - June 8, 2020 Category: Biochemistry Authors: Zeng S, Xing S, An F, Yang X, Yan J, Yu L, Luo L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

miR-20b-5p attenuates hypoxia-induced apoptosis in cardiomyocytes via the HIF-1 α/NF-κB pathway.
In conclusion, we identified that miR-20b-5p plays an important role in mediating cardiomyocytes apoptosis under hypoxia, which is mediated by the HIF-1/NF-κB signaling pathway. PMID: 32510153 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - June 8, 2020 Category: Biochemistry Authors: Zhou Z, Chen S, Tian Z, Deng S, Yi X, Yang S, Yang X, Jin L, Shi W Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

The application of ubiquitin ligases in the PROTAC drug design.
Abstract Protein ubiquitylation plays important roles in many biological activities. Protein ubiquitylation is a unique process that is mainly controlled by ubiquitin ligases. The ubiquitin-proteasome system (UPS) is the main process to degrade short-lived and unwanted proteins in eukaryotes. Many components in the UPS are attractive drug targets. Recent studies indicated that ubiquitin ligases can be employed as tools in proteolysis-targeting chimeras (PROTACs) for drug discovery. In this review article, we will discuss the recent progress of the application of ubiquitin ligases in the PROTAC drug design. We will...
Source: Acta Biochimica et Biophysica Sinica - June 6, 2020 Category: Biochemistry Authors: Chen Y, Jin J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Liver cancer stem cells as a hierarchical society: yes or no?
Abstract Cancer stem cells (CSCs) are cells possessing abilities of self-renewal, differentiation, and tumorigenicity in NOD/SCID mice. Based on this definition, multiple cell surface markers (such as CD24, CD133, CD90, and EpCAM) as well as chemical methods are discovered to enrich liver CSCs in the recent decade. Accumulated studies have revealed molecular signatures and signaling pathways involved in regulating different liver CSCs. Among liver CSCs positive for different markers, some molecular features and regulatory pathways are commonly shared, while some are only unique in certain CSC populations. These st...
Source: Acta Biochimica et Biophysica Sinica - June 3, 2020 Category: Biochemistry Authors: Gu Y, Zheng X, Ji J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

GPR146: an emerging therapeutic target for hypercholesterolemia and atherosclerosis.
PMID: 32491159 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - June 3, 2020 Category: Biochemistry Authors: She L, Yao H, He L, Li Y, Cao J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Role and effect of vein-transplanted human umbilical cord mesenchymal stem cells in the repair of diabetic foot ulcers in rats.
In this study, a rat DFU model was used to simulate the pathophysiology of clinical patients and to investigate the localization of human umbilical cord mesenchymal stem cells (hUC-MSCs) after intravenous transplantation and its role in DFU healing, so as to evaluate the potential of hUC-MSCs in the treatment of DFU. The diabetic rat model was established by streptozotocin injection, which was used to create full-thickness foot dorsal skin wounds to mimic DFU by a 6-mm skin biopsy punch and a Westcott scissor. The hUC-MSCs were transplanted through femoral vein, and the ulcer cicatrization situation and the fate of hUC-MSC...
Source: Acta Biochimica et Biophysica Sinica - June 2, 2020 Category: Biochemistry Authors: Shi R, Lian W, Jin Y, Cao C, Han S, Yang X, Zhao S, Li M, Zhao H Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

TBK1, a central kinase in innate immune sensing of nucleic acids and beyond.
Abstract Sensing of intracellular and extracellular environments is one of the fundamental processes of cell. Surveillance of aberrant nucleic acids, derived either from invading pathogens or damaged organelle, is conducted by pattern recognition receptors (PRRs) including RIG-I-like receptors, cyclic GMP-AMP synthase, absent in melanoma 2, and a few members of toll-like receptors. TANK-binding kinase 1 (TBK1), along with its close analogue I-kappa-B kinase epsilon, is a central kinase in innate adaptor complexes linking activation of PRRs to mobilization of transcriptional factors that transcribe proinflammatory ...
Source: Acta Biochimica et Biophysica Sinica - May 27, 2020 Category: Biochemistry Authors: Zhou R, Zhang Q, Xu P Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Regulation and functions of the Hippo pathway in stemness and differentiation.
Abstract The Hippo pathway plays important roles in organ development, tissue regeneration, and human diseases, such as cancer. In the canonical Hippo pathway, the MST1/2-LATS1/2 kinase cascade phosphorylates and inhibits transcription coactivators Yes-associated protein and transcription coactivator with PDZ-binding motif and thus regulates transcription of genes important for cell proliferation and apoptosis. However, recent studies have depicted a much more complicate picture of the Hippo pathway with many new components and regulatory stimuli involving both chemical and mechanical signals. Furthermore, accumul...
Source: Acta Biochimica et Biophysica Sinica - May 23, 2020 Category: Biochemistry Authors: Cao X, Wang C, Liu J, Zhao B Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Production of integrin αIIbβ3 in stably transfected and clonal Chinese hamster ovary cells for functional and structural studies.
Production of integrin αIIbβ3 in stably transfected and clonal Chinese hamster ovary cells for functional and structural studies. Acta Biochim Biophys Sin (Shanghai). 2020 May 23;: Authors: Pan L, Lu Y, Zuo Y, Qu K, Ma W, Liu J PMID: 32445462 [PubMed - as supplied by publisher] (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - May 23, 2020 Category: Biochemistry Authors: Pan L, Lu Y, Zuo Y, Qu K, Ma W, Liu J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Recent advances and potential applications of human pluripotent stem cell-derived pancreatic β cells.
Recent advances and potential applications of human pluripotent stem cell-derived pancreatic β cells. Acta Biochim Biophys Sin (Shanghai). 2020 May 23;: Authors: Zhou Z, Ma X, Zhu S Abstract Diabetes mellitus is characterized by chronic high blood glucose levels resulted from deficiency and/or dysfunction of insulin-producing pancreatic β cells. Generation of large amounts of functional pancreatic β cells is critical for the study of pancreatic biology and treatment of diabetes. Recent advances in directed differentiation of pancreatic β-like cells from human pluripotent stem cells...
Source: Acta Biochimica et Biophysica Sinica - May 23, 2020 Category: Biochemistry Authors: Zhou Z, Ma X, Zhu S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Regulation of alveolar type 2 stem/progenitor cells in lung injury and regeneration.
Abstract The renewal of lung epithelial cells is normally slow unless the lung is injured. The resident epithelial stem cells rapidly proliferate and differentiate to maintain lung structure and function when the lung is damaged. The alveolar epithelium is characterized by alveolar type 1 (AT1) and alveolar type 2 (AT2) cells. AT2 cells are the stem cells for alveoli, as they can both self-renew and generate AT1 cells. Abnormal proliferation and regulation of AT2 cells will lead to serious lung diseases including cancers. In this review, we focused on the alveolar stem/progenitor cells, the key physiological funct...
Source: Acta Biochimica et Biophysica Sinica - May 23, 2020 Category: Biochemistry Authors: Wu A, Song H Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Nkx2.1 downregulation is involved in brain abnormality induced by excess retinoic acid.
Abstract Abnormal development of central nervous system (CNS) caused by neural tube defects is not only a major contributor in the prevalence of stillbirths and neonatal deaths but also causes lifelong physical disability in surviving infants. Due to insufficient known investigated causes, CNS developmental abnormality has brought sever burden on health around the world. From previous results of high throughput transcriptome sequencing, we selected transcription factor Nkx2.1 as a candidate to investigate its role on brain abnormalities induced by excessive retinoic acid. The result of in situ hybridization showed...
Source: Acta Biochimica et Biophysica Sinica - May 23, 2020 Category: Biochemistry Authors: Jia S, Zhang L, Zhang K, Wang L, Khan A, Zhang J, Sun Y, Wang Y, Song M, Lyu Y, Li M, Lu X, Niu B, Liu Z, Xie J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

The essential function of IL-33 in metabolic regulation.
Abstract Interleukin-33 (IL-33) is produced by various types of cells under physical or pathological conditions. As a multifunctional partner in health and disease, current evidence reveals that IL-33 also participates in several metabolic processes. IL-33 has been proven to contribute to regulating the activity of ST2+ group 2 innate lymphoid cells and regulatory T cells in adipose, which leads to the shift of insulin sensitivity and glucose clearance in glucose metabolism, thermogenesis, and adipocyte beiging in adipose metabolism. In this review, we briefly summarize the biological characteristics of Il-33 and ...
Source: Acta Biochimica et Biophysica Sinica - May 22, 2020 Category: Biochemistry Authors: Li W, Li Y, Jin J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Effect of TrkB-PLC/IP3 pathway on intestinal inflammatory factors and enterocyte apoptosis in mice with colitis.
In this study, we aimed to explore the effect of TrkB-PLC/IP3 pathway on intestinal inflammatory factors and enterocyte apoptosis in mice with colitis. The mouse model of ulcerative colitis was established by medication, and 40 SPF C57BL/6J mice (8 weeks old) were randomly divided into normal group (healthy mice, n = 10), control group (sham-operated mice, n = 10), model group (model mice without any treatment, n = 10), and K252a group (model mice treated with 100 μmol/kg TrkB-PLC/IP3 pathway inhibitor for 5 days before clysis, n = 10). The results s...
Source: Acta Biochimica et Biophysica Sinica - May 22, 2020 Category: Biochemistry Authors: Xu G, Sun Y, He H, Xue Q, Liu Y, Dong L Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Parkin deficiency accentuates chronic alcohol intake-induced tissue injury and autophagy defects in brain, liver and skeletal muscle.
This study aimed to examine the effect of parkin deficiency on chronic alcohol intake-induced organ injury in brain, liver and skeletal muscle (rectus femoris muscle). Adult parkin-knockout (PRK-/-) and wild-type mice were placed on Liber-De Carli alcohol liquid diet (4%) for 12 weeks prior to assessment of liver enzymes, intraperitoneal glucose tolerance, protein carbonyl content, apoptosis, hematoxylin and eosin morphological staining, and mitochondrial respiration (cytochrome c oxidase, NADH:cytochrome c reductase and succinate:cytochrome c reductase). Autophagy protein markers were monitored by western blot analys...
Source: Acta Biochimica et Biophysica Sinica - May 19, 2020 Category: Biochemistry Authors: Peng H, Qin X, Chen S, Ceylan AF, Dong M, Lin Z, Ren J Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research