Query: siRNA

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Total 15785 results found since Jan 2013.

Cancer upregulated gene (CUG)2 elevates YAP1 expression, leading to enhancement of epithelial-mesenchymal transition in human lung cancer cells.
Abstract Although our previous studies have showed that a novel oncogene, cancer upregulated gene (CUG)2 induced epithelial-mesenchymal transition (EMT), the detailed molecular mechanism remains unknown. Because several lines of evidence documented that Yes-Associated Protein (YAP)1 is closely associated with cancer stem cell (CSC)-like phenotypes including EMT, stemness, and drug resistance, we wondered if YAP1 is involved in CUG2-induced EMT. We herein found that the overexpression of CUG2 increased YAP1 expression at the transcriptional as well as protein levels. Chromatin immunoprecipitation assay revealed tha...
Source: Biochemical and Biophysical Research communications - February 13, 2019 Category: Biochemistry Authors: Kaowinn S, Yawut N, Koh SS, Chung YH Tags: Biochem Biophys Res Commun Source Type: research

Up-regulation of C5orf34 promotes lung adenocarcinoma migration and is correlated with worse prognosis.
CONCLUSION: Our results firstly revealed that C5orf34 might play a facilitating role in LAD development and progression by regulating MAPK signaling pathway. Furthermore, our data implied that C5orf34 may be a potential predictor and treatment target for LAD. PMID: 30771479 [PubMed - as supplied by publisher]
Source: Gene - February 13, 2019 Category: Genetics & Stem Cells Authors: He D, Wu Z, He J, Wang Y, Li Z, Gao S Tags: Gene Source Type: research

Complement C3a receptor antagonist attenuates tau hyperphosphorylation via glycogen synthase kinase 3 β signaling pathways.
In this study, we found that exposure of SH-SY5Y cells to okadaic acid (OA) decreased cell viabilities and induced tau hyperphosphorylation. These effects were alleviated by C3a receptor antagonist SB290157 and were further validated by C3a receptor siRNA in OA-treated SH-SY5Y cells. In addition, our results demonstrated that SB290157 markedly inhibited the activities of glycogen synthase kinase 3β (GSK3β), but had no effect on protein phosphatase 2AC subunit (PP2Ac) and cyclin-dependent kinases 5 (CDK5). Our findings here indicate the unique role of the C3a receptor in regulating tau phosphorylation via GSK3β signaling...
Source: European Journal of Pharmacology - February 13, 2019 Category: Drugs & Pharmacology Authors: Hu J, Yang Y, Wang M, Yao Y, Chang Y, He Q, Ma R, Li G Tags: Eur J Pharmacol Source Type: research

Lysophosphatidic acid receptor 1 inhibitor, AM095, attenuates diabetic nephropathy in mice by downregulation of TLR4/NF-κB signaling and NADPH oxidase
In conclusion, AM095 is effective in preventing the pathogenesis of DN by inhibiting TLR4/NF-κB and the NADPH oxidase system, consequently inhibiting the inflammatory signaling cascade in renal tissue of diabetic mice, suggesting that LPAR1 antagonism might provide a potential therapeutic target for DN.
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - February 12, 2019 Category: Molecular Biology Source Type: research

Endoplasmic reticulum stress-dependent autophagy inhibits glycated high-density lipoprotein-induced macrophage apoptosis by inhibiting CHOP pathway.
This study was designed to explore the inductive effect of glycated high-density lipoprotein (gly-HDL) on endoplasmic reticulum (ER) stress-C/EBP homologous protein (CHOP)-mediated macrophage apoptosis and its relationship with autophagy. Our results showed that gly-HDL caused macrophage apoptosis with concomitant activation of ER stress pathway, including nuclear translocation of activating transcription factor 6, phosphorylation of protein kinase-like ER kinase (PERK) and eukaryotic translation initiation factor 2α, and CHOP up-regulation, which were inhibited by 4-phenylbutyric acid (PBA, an ER stress inhibitor) and th...
Source: J Cell Mol Med - February 12, 2019 Category: Molecular Biology Authors: Tian H, Li Y, Kang P, Wang Z, Yue F, Jiao P, Yang N, Qin S, Yao S Tags: J Cell Mol Med Source Type: research

Activation of T Lymphocytes as a Novel Mechanism in Beta1-Adrenergic Receptor Autoantibody-Induced Cardiac Remodeling
ConclusionsCollectively, we demonstrate that β1-AAmAb-induced cardiac remodeling via mediating T lymphocyte disorder and releasing a variety of IL-6.
Source: Cardiovascular Drugs and Therapy - February 12, 2019 Category: Cardiology Source Type: research

p53-Mediated activities in NS-5 neural stem cells: effects of ethanol.
CONCLUSIONS: Thus, it appears that ethanol and TGFβ1 affect proliferation, DNA repair, and survival of NSCs via p53-mediated activities. This article is protected by copyright. All rights reserved. PMID: 30748015 [PubMed - as supplied by publisher]
Source: Alcoholism, Clinical and Experimental Research - February 12, 2019 Category: Addiction Authors: Miller MW Tags: Alcohol Clin Exp Res Source Type: research

Protective effects of ethyl gallate on H 2 O 2 -induced mitochondrial dysfunction in PC12 cells
AbstractOxidative stress has been suggested to play an important role in neuronal injury. Ethyl gallate (EG) is the ethyl ester of gallic acid which has been acknowledged as an antioxidant. We previously demonstrated that EG effectively inhibited H2O2-induced cytotoxicity and decreased the ROS levels in PC12 cells, while the relevant mechanisms of action of this compound remain largely uncharacterized. The present study was carried out in an attempt to clarify the underlying mechanisms of EG against H2O2-induced neurotoxicity in PC12 cells. EG pretreatment attenuated H2O2-induced mitochondrial dysfunction as indicated by t...
Source: Metabolic Brain Disease - February 12, 2019 Category: Neurology Source Type: research

Curcumin Alleviates β Amyloid-Induced Neurotoxicity in HT22 Cells via Upregulating SOD2
In this study, the HT22 neuronal cells were exposed to Aβ to imitate neuronal injury in Alzheimer’s disease (AD). After 24-h treatment, 10 μM Aβ decreased cell viability and mitochondrial functions, including mitochondrial complex activities and mitochondrial membrane potential (MMP), and also downregulated anti-oxidants SOD2, glutathione (GSH), and catalase (CAT) levels (P <  0.05), meanwhile, increased lactic dehydrogenase (LDH) release, apoptosis level, intracellular reactive oxygen species (ROS) and mitochondrial superoxide accumulation (P <  0.05). And, co-administration of 1 μM curcumin signific...
Source: Journal of Molecular Neuroscience - February 12, 2019 Category: Neuroscience Source Type: research

A minimalist's approach for DNA nanoconstructions.
Abstract Structural DNA nanotechnology takes DNA, a biopolymer, far beyond being the molecule that stores and transmits genetic information in biological systems. DNA has been employed as building blocks for the assembly of designed, nanoscaled, supramolecular DNA architectures for applications in biophysics, structure determination, synthetic biology, diagnostics, and drug delivery. Herein, we review a symmetric approach of tile-based DNA self-assembly. This approach allows construction of DNA nanostructures from minimal numbers of different types of DNA strands based on sequence and structural symmetries. Some e...
Source: Advanced Drug Delivery Reviews - February 12, 2019 Category: Drugs & Pharmacology Authors: Zuo H, Mao C Tags: Adv Drug Deliv Rev Source Type: research

Nuclear accumulation of UBC9 contributes to SUMOylation of lamin A/C and nucleophagy in response to DNA damage.
CONCLUSIONS: Our findings suggest that nuclear DNA leakage activates nucleophagy through UBC9-mediated SUMOylation of lamin A/C, leading to degradation of nuclear components including lamin A/C and leaked nuclear DNA. PMID: 30744690 [PubMed - in process]
Source: Clinical Breast Cancer - February 11, 2019 Category: Cancer & Oncology Authors: Li Y, Jiang X, Zhang Y, Gao Z, Liu Y, Hu J, Hu X, Li L, Shi J, Gao N Tags: J Exp Clin Cancer Res Source Type: research

Adeno-associated virus 9-mediated RNA interference targeting SOCS3 alleviates diastolic heart failure in rats.
CONCLUSIONS: SOCS3 gene silencing by AAV9-SOCS3 siRNA administration in a DHF rat model significantly reduced myocardial fibrosis and the inflammatory response and improved heart function. Therefore, this treatment is a potential therapeutic method for treating DHF. PMID: 30763670 [PubMed - as supplied by publisher]
Source: Gene - February 11, 2019 Category: Genetics & Stem Cells Authors: Gao J, Guo Y, Chen Y, Zhou J, Liu Y, Su P Tags: Gene Source Type: research

The circular RNome of primary breast cancer RESEARCH
Circular RNAs (circRNAs) are a class of RNAs that is under increasing scrutiny, although their functional roles are debated. We analyzed RNA-seq data of 348 primary breast cancers and developed a method to identify circRNAs that does not rely on unmapped reads or known splice junctions. We identified 95,843 circRNAs, of which 20,441 were found recurrently. Of the circRNAs that match exon boundaries of the same gene, 668 showed a poor or even negative (R < 0.2) correlation with the expression level of the linear gene. In silico analysis showed only a minority (8.5%) of circRNAs could be explained by known splicing events...
Source: Genome Research - February 11, 2019 Category: Genetics & Stem Cells Authors: Smid, M., Wilting, S. M., Uhr, K., Rodriguez-Gonzalez, F. G., de Weerd, V., Prager-Van der Smissen, W. J. C., van der Vlugt-Daane, M., van Galen, A., Nik-Zainal, S., Butler, A., Martin, S., Davies, H. R., Staaf, J., van de Vijver, M. J., Richardson, A. L. Tags: RESEARCH Source Type: research

TGF- β1 Decreases β2-Agonist-Induced Relaxation in Human Airway Smooth Muscle.
TGF-β1 Decreases β2-Agonist-Induced Relaxation in Human Airway Smooth Muscle. Am J Respir Cell Mol Biol. 2019 Feb 11;: Authors: Ojiaku CA, Chung E, Parikh V, Williams JK, Schwab A, Fuentes AL, Corpuz ML, Lui V, Paek S, Bexiga NM, Narayan S, Nunez FJ, Ahn K, Ostrom RS, An SS, Panettieri RA Abstract Helper T effector cytokines implicated in asthma modulate the contractility of human airway smooth muscle (HASM) cells. We have reported recently that a profibrotic cytokine, transforming growth factor beta 1 (TGF-β1), induces HASM cell shortening and airway hyper-responsiveness (AHR). Here we assessed wh...
Source: American Journal of Respiratory Cell and Molecular Biology - February 11, 2019 Category: Molecular Biology Authors: Ojiaku CA, Chung E, Parikh V, Williams JK, Schwab A, Fuentes AL, Corpuz ML, Lui V, Paek S, Bexiga NM, Narayan S, Nunez FJ, Ahn K, Ostrom RS, An SS, Panettieri RA Tags: Am J Respir Cell Mol Biol Source Type: research

NOTCH3 overexpression and post-transcriptional regulation by miR-150 were associated with EGFR-TKI resistance in lung adenocarcinoma.
Authors: Zhang Y, Chen B, Wang Y, Zhao Q, Wu W, Zhang P, Miao L, Sun S Abstract Acquired resistance remains a key challenge in epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKI) therapy in lung adenocarcinoma (LUAD). Recent studies have shown that Notch signaling is associated with drug resistance. However, its role and possible mechanisms in EGFR-TKI resistance are not yet clear. In our study, we found that among 4 members of NOTCH1-4, only NOTCH3 was up-regulated in LUAD tissues and TKI-resistant cell line (HCC827GR6). Knockdown of NOTCH3 by siRNA significantly inhibited proliferative abilit...
Source: Oncology Research - February 10, 2019 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Cancers, Vol. 11, Pages 202: SYK Inhibition Potentiates the Effect of Chemotherapeutic Drugs on Neuroblastoma Cells in Vitro
In this study, we observed elevated SYK gene expression in neuroblastoma compared to neural crest and benign neurofibroma. While SYK protein was detected in the majority of examined neuroblastoma tissues it was less frequently observed in neuroblastoma cell lines. Depletion of SYK by siRNA and the use of small molecule SYK inhibitors significantly reduced the cell viability of neuroblastoma cell lines expressing SYK protein. Moreover, SYK inhibition decreased ERK1/2 and Akt phosphorylation. The SYK inhibitor BAY 613606 enhanced the effect of different chemotherapeutic drugs. Transient expression of a constitutive active SY...
Source: Cancers - February 10, 2019 Category: Cancer & Oncology Authors: Conny T ümmler Gianina Dumitriu Malin Wickstr öm Peter Coopman Andrey Valkov Per Kogner John Inge Johnsen Ugo Moens Baldur Sveinbj örnsson Tags: Article Source Type: research

Nr5a2 promotes cancer stem cell properties and tumorigenesis in nonsmall cell lung cancer by regulating Nanog
In the present study, we have provided convincing evidence in vitro and in vivo demonstrating thatNr5a2 can induce lung cancer stem cells (CSC) properties and promote tumorigenesis and progression through transcriptional up ‐regulation of Nanog. AbstractLung cancer has the highest mortality rate due to late diagnosis and high incidence of metastasis. Cancer stem cells (CSCs) are a subgroup of cancer cells with self ‐renewal capability similar to that of normal stem cells (NSCs). While CSCs may play an important role in cancer progression, mechanisms underlying CSC self‐renewal and the relationship between self‐rene...
Source: Cancer Medicine - February 10, 2019 Category: Cancer & Oncology Authors: Ting Ye, Jingyuan Li, Zhiwei Sun, Yongli Liu, Liangsheng Kong, Shixia Zhou, Junlin Tang, Jianyu Wang, H. Rosie Xing Tags: ORIGINAL RESEARCH Source Type: research

Nox1/Ref-1-Mediated Activation of CREB Promotes Gremlin1-Driven Endothelial Cell Proliferation & Migration
Publication date: Available online 8 February 2019Source: Redox BiologyAuthor(s): Daniel S. de Jesus, Evan DeVallance, Yao Li, Micol Falabella, Danielle Guimaraes, Sruti Shiva, Brett Kaufman, Mark T. Gladwin, Patrick J. PaganoAbstractPulmonary arterial hypertension (PAH) is a complex degenerative disorder marked by aberrant vascular remodeling associated with hyperproliferation and migration of endothelial cells (ECs). Previous reports implicated bone morphogenetic protein antagonist Gremlin 1 in this process; however, little is known of the molecular mechanisms involved. The current study was designed to test whether redo...
Source: Redox Biology - February 9, 2019 Category: Biology Source Type: research

Protective Effects of Broussonetia kazinoki Siebold Fruit Extract against Palmitate-Induced Lipotoxicity in Mesangial Cells.
In conclusion, BKFE induced the expression of antioxidant enzymes via activation of Nrf2 and protected against PA-induced lipotoxicity in mesangial cells. PMID: 30728845 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - February 9, 2019 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

Fli-1 transcription factor regulates the expression of caspase-1 in lung pericytes
Publication date: April 2019Source: Molecular Immunology, Volume 108Author(s): Pengfei Li, Andrew J. Goodwin, James A. Cook, Perry V. Halushka, Xian K. Zhang, Hongkuan FanAbstractOur previous data demonstrated that Friend leukemia virus integration 1 (Fli-1), an ETS transcription factor, governs pericyte loss and vascular dysfunction in cecal ligation and puncture-induced murine sepsis by regulating essential pyroptosis markers including caspase-1. However, whether Fli-1 regulates caspase-1 expression levels in vitro and how Fli-1 regulates caspase-1 remain unknown. Our present work further demonstrated that overexpressed ...
Source: Molecular Immunology - February 9, 2019 Category: Allergy & Immunology Source Type: research

HIPK1 Interference Attenuates Inflammation and Oxidative Stress of Acute Lung Injury via Autophagy.
CONCLUSIONS</strong> Decreased HIPK1 in ALI showed protective effects in attenuating inflammation and oxidative stress and enhancing autophagy, indicating HIPK1 as a possible target in ALI management. PMID: 30734722 [PubMed - in process]
Source: Medical Science Monitor - February 9, 2019 Category: Research Tags: Med Sci Monit Source Type: research

Translocator protein 18 kDa ligand alleviates neointimal hyperplasia in the diabetic rat artery injury model via activating PKG
This study suggests that TSPO inhibition suppresses the proliferation and migration of VSMCs induced by hyperglycemia, consequently, preventing atherosclerosis and restenosis after angioplasty in diabetic conditions. TSPO may be a potential therapeutic target to reduce arterial remodeling induced by angioplasty in diabetes.
Source: Life Sciences - February 9, 2019 Category: Biology Source Type: research

Piperlongumine-induced nuclear translocation of the FOXO3A transcription factor triggers BIM-mediated apoptosis in cancer cells.
We report here that an association of FOXO3A with the pro-apoptotic protein BIM (also known as BCL2-like 11, BCL2L11) has a direct and specific function in PL-induced cancer cell death. Using HeLa cells stably expressing a FOXO3A-GFP fusion protein and several other cancer cell lines, we found that PL treatment induces FOXO3A dephosphorylation and nuclear translocation and promotes its binding to the BIM gene promoter, resulting in the up-regulation of BIM in the cancer cell lines. Accordingly, PL inhibited cell viability and caused intrinsic apoptosis in a FOXO3A-dependent manner. Of note, siRNA-mediated FOXO3A knockdown ...
Source: Biochemical Pharmacology - February 9, 2019 Category: Drugs & Pharmacology Authors: Liu Z, Shi Z, Lin J, Zhao S, Hao M, Xu J, Li Y, Zhao Q, Tao L, Diao A Tags: Biochem Pharmacol Source Type: research

Co-targeting RNA Polymerases IV and V Promotes Efficient De Novo DNA Methylation in Arabidopsis
Publication date: Available online 7 February 2019Source: CellAuthor(s): Javier Gallego-Bartolomé, Wanlu Liu, Peggy Hsuanyu Kuo, Suhua Feng, Basudev Ghoshal, Jason Gardiner, Jenny Miao-Chi Zhao, Soo Young Park, Joanne Chory, Steven E. JacobsenSummaryThe RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression. Here, we identified diverse RdDM proteins that are capable of targeting methylation and silencing in Arabidopsis when tethered to an art...
Source: Cell - February 8, 2019 Category: Cytology Source Type: research

Genistein suppresses psoriasis-related inflammation through a STAT3-NF- κB-dependent mechanism in keratinocytes.
In this study, we investigated the effects of genistein on imiquimod (IMQ)-induced psoriasis-like skin lesions in vivo and explored the mechanisms underlying those effects in vitro. It was found that genistein can significantly improve IMQ-induced pathological scores of cutaneous skin lesions in mice, reduce epidermal thickness, and inhibit the expression of inflammatory factors,including interleukin (IL)-1β, IL-6, tumour necrosis factor-alpha (TNF-α), chemokine ligand 2 (CCL2), IL-17 and IL-23. In vitro studies, genistein inhibited the proliferation of human keratinocyte HaCaT cells and inhibited the expression of inf...
Source: International Immunopharmacology - February 8, 2019 Category: Allergy & Immunology Authors: Wang A, Wei J, Lu C, Chen H, Zhong X, Lu Y, Li L, Huang H, Dai Z, Han L Tags: Int Immunopharmacol Source Type: research

Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment
This study provides important sex- and tissue-specific expression data of miRNA, piRNA and siRNA as well as mechanistic insights into sex-differences of gene regulation in response to environmental cues.
Source: PLoS Genetics - February 8, 2019 Category: Genetics & Stem Cells Authors: Alexandra Bezler Source Type: research

Baicalin induced colon cancer cells apoptosis through miR-217/DKK1-mediated inhibition of Wnt signaling pathway.
Abstract To analyze the anti-tumor mechanism of Baicalin in human colon cancer. The MTT assay and colony formation assay demonstrated that Baicalin treatment inhibits the proliferation of DLD1 and HCT-116 cells. The apoptosis rates were induced upon Baicalin treatment and which was determined by FACS. The qPCR and western blot analysis showed that Baicalin promotes expression of DKK1 (Dickkopf), an important antagonist of Wnt signaling pathway, thereby reduces the expression of its downstream protein β-catenin and c-Myc. Reduction of DKK1 expression by siRNA attenuates β-catenin and c-Myc expression. microRNA-21...
Source: Molecular Biology Reports - February 8, 2019 Category: Molecular Biology Authors: Jia Y, Chen L, Guo S, Li Y Tags: Mol Biol Rep Source Type: research

CircRNA hsa-circ-0014359 promotes glioma progression by regulating miR-153/PI3K signaling.
Abstract RNA back splicing produces circRNA, a new type of non-coding RNA. Studies have indicated that circRNAs play important roles in malignant tumors of the central nervous system. Here, we aimed to evaluate the expression of the circRNA, hsa-circ-0014359 (circ-0014359) in human glioma cell lines to assess its function in glioma progression and prognosis. The expression of circ-0014359 was increased in T98G and SHG44 cancer cell lines and glioma tissues from patients, when compared with control cells and tissue. SiRNA-mediated silencing of circ-0014359 potently inhibited cell viability, migration, invasion, and...
Source: Biochemical and Biophysical Research communications - February 8, 2019 Category: Biochemistry Authors: Shi F, Shi Z, Zhao Y, Tian J Tags: Biochem Biophys Res Commun Source Type: research

The arginine methyltransferase PRMT5 and PRMT1 distinctly regulate the degradation of anti-apoptotic protein CFLARL in human lung cancer cells.
CONCLUSIONS: PRMT5 and PRMT1 mediate the distinct effects on CFLARL degradation by regulating the binding of E3 ligase ITCH in NSCLC cells. This study identifies a cell death mechanism that is fine-tuned by PRMT1/5 that modulate CFLARL degradation in human NSCLC cells. PMID: 30736843 [PubMed - in process]
Source: Clinical Lung Cancer - February 8, 2019 Category: Cancer & Oncology Authors: Li M, An W, Xu L, Lin Y, Su L, Liu X Tags: J Exp Clin Cancer Res Source Type: research

Mucin 1 downregulation impairs the anti-necroptotic effects of glucocorticoids in human bronchial epithelial cells
Publication date: Available online 7 February 2019Source: Life SciencesAuthor(s): Huojun Zhang, Qian Liu, Luxia Kong, Shuyun XuAbstractAimsTo investigate whether mucin 1 (MUC1) downregulation reduced the sensitivity of tumor necrosis factor-alpha (TNF-α)-induced bronchial epithelial cells to glucocorticoid-mediated necroptosis and explore the underlying mechanisms.Main methodsThe human lung bronchial epithelial cell line (16HBE) was transfected with small interfering RNA (siRNA) against MUC1 and then stimulated by TNF-α, where some cells were pretreated with dexamethasone. Flow cytometry was performed to analyze necropto...
Source: Life Sciences - February 7, 2019 Category: Biology Source Type: research

The role of the fat mass and obesity-associated protein in the proliferation of pancreatic cancer cells.
Authors: Tang X, Liu S, Chen D, Zhao Z, Zhou J Abstract Fat mass and obesity-associated (FTO) protein has been identified as a critical demethylase in regulating cellular mRNA stability by removing N6-methyladenosine (m6A) residues in mRNA. Even though the role of FTO in body energy metabolism has been well established, its role in cancer cell homeostasis remains unclear. In the present study, by using RNA interference, it was indicated that FTO is required for pancreatic cancer cell proliferation. Knockdown of FTO resulted in compromised proliferation of pancreatic cancer cells. Furthermore, DNA synthesis was comp...
Source: Oncology Letters - February 7, 2019 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Cellular and radiobiological effects of carbonic anhydrase IX in human breast cancer cells.
In conclusion, combination of CAIX inhibition and irradiation is a promising treatment strategy against breast cancer with hypoxia‑induced CAIX expression. PMID: 30720123 [PubMed - as supplied by publisher]
Source: Oncology Reports - February 7, 2019 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

TSPAN1 upregulates MMP2 to promote pancreatic cancer cell migration and invasion via PLC γ.
TSPAN1 upregulates MMP2 to promote pancreatic cancer cell migration and invasion via PLCγ. Oncol Rep. 2019 Jan 30;: Authors: Zhang X, Shi G, Gao F, Liu P, Wang H, Tan X Abstract Pancreatic cancer (PC), a malignancy of the digestive system, has one of the highest rates of metastasis and mortality. It is characterized by the detachment, migration, implantation and infiltration of tumor cells to form metastases or recurrent foci. Tetraspanin 1 (TSPAN1), a novel member of the TSPAN superfamily, is highly expressed in many types of cancer, including gastric, colon, liver and esophageal cancer. It has also ...
Source: Oncology Reports - February 7, 2019 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MYC promotes the development of papillary thyroid carcinoma by inhibiting the expression of lncRNA PAX8 ‑AS1:28.
MYC promotes the development of papillary thyroid carcinoma by inhibiting the expression of lncRNA PAX8‑AS1:28. Oncol Rep. 2019 Feb 01;: Authors: Zhang Y, Li F, Chen J Abstract As a common malignancy of the endocrine system, papillary thyroid carcinoma (PTC) seriously affects the quality of life of patients. lncRNA PAX8‑AS1:28, or lnc‑PSD4‑1:14 has been reported to be abnormally expressed in PTC. However, the function of PAX8‑AS1:28 in PTC is still unknown. Therefore, the present study aimed to investigate the functions of PAX8‑AS1:28 in PTC, and to explore the possible mechanisms of action...
Source: Oncology Reports - February 7, 2019 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

SPAG9/MKK3/p38 axis is a novel therapeutic target for liver cancer.
Authors: Luo S, Ren B, Zou G, Liu J, Chen W, Huang Y, Chen X, Fu Y Abstract Sperm‑associated antigen 9 (SPAG9) is a biomarker and potential therapeutic target for several cancers; however, its involvement in liver cancer progression is not clear. The aim of the present study was to determine whether SPAG9 regulates proliferation of liver cancer. Immunohistochemistry and cell immunofluorescence were used to confirm the expression and the localization of SPAG9 in human liver cancer tissues and the liver cancer‑derived HepG2 cells. A small interfering RNA (siRNA) designed to target SPAG9 was transiently transfecte...
Source: Oncology Reports - February 7, 2019 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Transducin-like enhancer of split 3 regulates proliferation of melanoma cells via histone deacetylase activity.
In conclusion, these data indicate that Tle3 is required, at least in part, for proliferation in the B16 mouse melanoma model. PMID: 30719233 [PubMed]
Source: Oncotarget - February 7, 2019 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

ATF4 contributes to autophagy and survival in sunitinib treated brain tumor initiating cells (BTICs).
Authors: Moeckel S, LaFrance K, Wetsch J, Seliger C, Riemenschneider MJ, Proescholdt M, Hau P, Vollmann-Zwerenz A Abstract Receptor tyrosine kinase (RTK) pathways are known to play an important role in tumor cell proliferation of glioblastoma (GBM). Cellular determinants of RTK-inhibitor sensitivity are important to optimize and tailor treatment strategies. The stress response gene activating transcription factor 4 (ATF4) is involved in homeostasis and cellular protection. However, little is known about its function in GBM. We found that the ATF4/p-eIF2α pathway is activated in response to Sunitinib in primary tum...
Source: Oncotarget - February 7, 2019 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Chemerin acts via CMKLR1 and GPR1 to stimulate migration and invasion of gastric cancer cells: putative role of decreased TIMP-1 and TIMP-2.
In this study we examined the expression of putative chemerin receptors in gastric cancer and the action of chemerin on cancer cell migration and invasion. Immunohistochemical studies of gastric tumors identified expression of two putative receptors, chemokine-like receptor-1 (CMKLR1) and G-protein coupled receptor 1(GPR1), in cancer cells; there was also some expression in stromal myofibroblasts although generally at a lower intensity. The expression of both receptors was detected in a gastric cancer cell line, AGS; chemerin itself was expressed in cultured gastric cancer myofibroblasts but not AGS cells. Chemerin stimula...
Source: Oncotarget - February 7, 2019 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells.
Authors: Ju F, Li N, Wang W, Yuan H Abstract Colorectal cancer (CRC), also known as bowel cancer, is one of the leading causes of cancer‑associated mortality worldwide at present. The aim of the present study was to detect the effects of matrix metalloproteinase 1 (MMP1) on the viability and migration of a CRC cell line in the presence or absence of variation X‑ray radiation doses. The CRC cell line, SW620, was cultured and treated with different X‑ray doses (0, 0.1, 0.5, 1, 3 and 6 Gy). MMP1 expression was downregulated via the application of a specific small interfering (si)‑RNA. The viability and migra...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

ASAP Transferrin-Appended Nanocaplet for Transcellular siRNA Delivery into Deep Tissues
Journal of the American Chemical SocietyDOI: 10.1021/jacs.8b12501
Source: Journal of the American Chemical Society - February 7, 2019 Category: Chemistry Authors: Ai Kohata, P. K. Hashim, Kou Okuro, Takuzo Aida Source Type: research

GSE124750 Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis ChIP-seq
Contributors : Javier Gallego-Bartolom é ; Wanlu LiuSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Arabidopsis thalianaThe RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression. Here, we identified diverse RdDM proteins that are capable of targeting methylation and silencing in Arabidopsis when tethered to an artificial zinc finger (ZF-RdDM). We studied their order of action within the RdDM pathway b...
Source: GEO: Gene Expression Omnibus - February 7, 2019 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

GSE124749 Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis small RNA-seq
Contributors : Javier Gallego-Bartolom é ; Wanlu LiuSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Arabidopsis thalianaThe RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression. Here, we identified diverse RdDM proteins that are capable of targeting methylation and silencing in Arabidopsis when tethered to an artificial zinc finger (ZF-RdDM). We studied their order of action within the RdDM pathway by testing ...
Source: GEO: Gene Expression Omnibus - February 7, 2019 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

GSE124747 Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis RNA-seq
Contributors : Javier Gallego-Bartolom é ; Wanlu LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Arabidopsis thalianaThe RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression. Here, we identified diverse RdDM proteins that are capable of targeting methylation and silencing in Arabidopsis when tethered to an artificial zinc finger (ZF-RdDM). We studied their order of action within the RdDM pathway by testing thei...
Source: GEO: Gene Expression Omnibus - February 7, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

GSE124746 Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis WGBS
Contributors : Javier Gallego-Bartolom é ; Wanlu LiuSeries Type : Methylation profiling by high throughput sequencingOrganism : Arabidopsis thalianaThe RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression. Here, we identified diverse RdDM proteins that are capable of targeting methylation and silencing in Arabidopsis when tethered to an artificial zinc finger (ZF-RdDM). We studied their order of action within the RdDM pathway by testing the...
Source: GEO: Gene Expression Omnibus - February 7, 2019 Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

GSE124744 Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis BS-PCR-seq
Contributors : Javier Gallego-Bartolom é ; Wanlu LiuSeries Type : Methylation profiling by high throughput sequencingOrganism : Arabidopsis thalianaThe RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression. Here, we identified diverse RdDM proteins that are capable of targeting methylation and silencing in Arabidopsis when tethered to an artificial zinc finger (ZF-RdDM). We studied their order of action within the RdDM pathway by testing the...
Source: GEO: Gene Expression Omnibus - February 7, 2019 Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

GSE124546 Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis
Contributors : Javier Gallego-Bartolom é ; Wanlu LiuSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencing ; Methylation profiling by high throughput sequencing ; Non-coding RNA profiling by high throughput sequencingOrganism :The RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression. Here, we identified diverse RdDM proteins that are capable of targeting methylatio...
Source: GEO: Gene Expression Omnibus - February 7, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Methylation profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing Source Type: research

MicroRNA-145 targets Smad1 in endometrial stromal cells and regulates decidualization in rat
In conclusion, the study demonstrated the role of miR-145 in regulation of progression of decidualization which is mediated through inhibition of Smad1.Key messagesMiR-145 expression is downregulated during decidualization in the rat uterus.Overexpression of miR-145 inhibited the decidualization progression.MiR-145 suppressed the migration and invasion of HUVECs.MiR-145 downregulated Smad1 which suppresses Smad1/5/8, Wnt-4, MMP-9, Cox-2, and VEGF.
Source: Journal of Molecular Medicine - February 7, 2019 Category: Molecular Biology Source Type: research

A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells
The small interfering RNA (siRNA) pathway is the main and best studied invertebrate antiviral response. Other poorly characterized protein based antiviral mechanisms also contribute to the control of viral replication in insects. In addition, it remains unclear whether tissue specific factors contribute to RNA and protein-based antiviral immunity mechanisms. In vivo screens to identify such factors are challenging and time consuming. In addition, the scored phenotype is usually limited to survival and/or viral load. Transgenic viral replicons are valuable tools to overcome these limitations and screen for novel antiviral f...
Source: G3: Genes Genomes Genetics - February 7, 2019 Category: Genetics & Stem Cells Authors: Martins, N., Lemoine, A., Santiago, E., Paro, S., Imler, J.-L., Meignin, C. Tags: Genetics of Immunity Source Type: research

CSF1R inhibitors exhibit antitumor activity in acute myeloid leukemia by blocking paracrine signals from support cells
This study identifies CSF1R as a novel therapeutic target of AML and provides a mechanism of paracrine cytokine/growth factor signaling in this disease.
Source: Blood - February 7, 2019 Category: Hematology Authors: Edwards, D. K., Watanabe-Smith, K., Rofelty, A., Damnernsawad, A., Laderas, T., Lamble, A., Lind, E. F., Kaempf, A., Mori, M., Rosenberg, M., dAlmeida, A., Long, N., Agarwal, A., Sweeney, D. T., Loriaux, M., McWeeney, S. K., Tyner, J. W. Tags: Myeloid Neoplasia Source Type: research