Query: siRNA

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Total 13806 results found since Jan 2013.

Tetrahydroxy Stilbene Glucoside Alleviates High Glucose-Induced MPC5 Podocytes Injury Through Suppression of NLRP3 Inflammasome.
CONCLUSIONS: TSG attenuates high glucose-induced cell apoptosis in vitro partly through the suppression of NLRP3 inflammasome signaling. PMID: 29891042 [PubMed - in process]
Source: The American Journal of the Medical Sciences - June 1, 2018 Category: General Medicine Authors: Li J, Wang B, Zhou G, Yan X, Zhang Y Tags: Am J Med Sci Source Type: research

Upregulation of microRNA-335-5p reduces inflammatory responses by inhibiting FASN through the activation of AMPK/ULK1 signaling pathway in a septic mouse model.
Abstract Sepsis, as a systemic inflammatory response syndrome (SIRS) subtype, is generally characterized by infection. Emerging evidence has highlighted dysregulated microRNAs (miRNAs) are involved in the progression of sepsis. The aim of the study was to investigate the effects of miR-335-5p on inflammatory responses in a septic mouse model. The hypothesis was subsequently asserted that the FASN gene and AMPK/ULK1 signaling pathway may participate in the regulation of miR-335-5p. A septic mouse model was established in order to validate the effect of miR-335-5p on the inflammatory response by means of suppressing...
Source: Cytokine - June 1, 2018 Category: Molecular Biology Authors: Gao XL, Li JQ, Dong YT, Cheng EJ, Gong JN, Qin YL, Huang YQ, Yang JJ, Wang SJ, An DD Tags: Cytokine Source Type: research

Insights into the role of estrogen-related receptors α, β and γ in tumor Leydig cells.
In this study, we demonstrate, for the first time, estrogen-related receptor (ERR) regulation of the physiological and biochemical status of testicular tumor Leydig cells. In a mouse tumor Leydig cells, ERRs (α, β, and γ) were silenced via siRNA. Cell morphology and cell physiology (proliferation and observation of monolayer formation) were performed by inverted phase-contrast microscope. Leydig cell functional markers (steroid receptors and signaling molecules) were examined by immunofluorescence and Western blotting. Additionally, progesterone secretion was assessed. Mitochondrial mass and membrane potential were anal...
Source: Tissue and Cell - June 1, 2018 Category: Cytology Authors: Kotula-Balak M, Milon A, Pawlicki P, Opydo-Chanek M, Pacwa A, Lesniak K, Sekula M, Zarzycka M, Bubka M, Tworzydlo W, Bilinska B, Hejmej A Tags: Tissue Cell Source Type: research

P90-RSK as a synthetic lethal target in pancreatic cancer - molecular characterization and therapeutic inhibition
Objectives Pancreatic cancer continues to be the cancer entity with the worst prognosis among all solid tumors associated with a 5-year survival of 5-8%. Combined chemotherapeutic regimes provide only survival benefits for a few months and are not equally suited for all patients with advanced pancreatic cancer. Targeted approaches have largely failed. A marginal clinical benefit was seen for an EGFR inhibitior, erlotinib, in combination with chemotherapy. Using a kinome-wide small-interfering RNA (siRNA)-based loss of function screen, we recently identified the kinase RPS6KA2, also known as p90 ribosomal S6 kinase RSK3, as...
Source: Pancreatology - June 1, 2018 Category: Gastroenterology Authors: Jan Riedel, Nada Milosevic, Jens von Kries, Marc Nazare, Heidi Griesmann, Patrick Michl Tags: 4. Experimental pancreatitis and cell biology II Source Type: research

RNAi-mediated SLC7A11 knockdown inhibits melanogenesis-related genes expression in rabbit skin fibroblasts.
Abstract Solute carrier family 7 member 11 (SLC7A11) is a cystine/glutamate exchanger, also known as xCT, has been found to play an important role in pheomelanin synthesis. Adjusting the cystine content of cells to influence pheomelanin synthesis affects the proportion of total melanin, changing mammalian coat colour. In our previous study, we used RNA-seq to show that SLC7A11 was involved in coat colour regulation in Rex rabbits. However, the precise role of SLC7A11 in rabbit coat colour formation has not been investigated. To better understand the functions of SLC7A11 in rabbits, we used RNA interference (RNAi) ...
Source: Journal of Genetics - June 1, 2018 Category: Genetics & Stem Cells Authors: Yang N, Mu L, Zhao B, Wang M, Hu S, Zhao B, Chen Y, Wu X Tags: J Genet Source Type: research

Role of MAPK/MNK1 signaling in virus replication.
Abstract Viruses are obligate intracellular parasites; they heavily depend on the host cell machinery to effectively replicate and produce new progeny virus particles. Following viral infection, diverse cell signaling pathways are initiated by the cells, with the major goal of establishing an antiviral state. However, viruses have been shown to exploit cellular signaling pathways for their own effective replication. Genome-wide siRNA screens have also identified numerous host factors that either support (proviral) or inhibit (antiviral) virus replication. Some of the host factors might be dispensable for the host ...
Source: Virus Research - June 1, 2018 Category: Virology Authors: Kumar R, Khandelwal N, Thachamvally R, Tripathi BN, Barua S, Kashyap SK, Maherchandani S, Kumar N Tags: Virus Res Source Type: research

p38 MAPK is Crucial for Wnt1- and LiCl-Induced Epithelial Mesenchymal Transition
SummaryIdiopathic pulmonary fibrosis (IPF) is characterized by myofibroblast foci in lung parenchyma. Myofibroblasts are thought to originate from epithelial-to-mesenchymal transition (EMT). Wnt1 and lithium chloride (LiCl) induce EMT in alveolar epithelial cells (AECs), but the mechanisms are unclear. AECs were treated with Wnt1 and LiCl, respectively; morphological change and molecular changes of EMT, including E-cadherin, fibronectin, and vimentin, were observed. SB203580 was administrated to test the role of p38 МАРК signaling in EMT. Then AECs were treated with siRNAs targeting p38 МАРК to further test the eff...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2018 Category: Research Source Type: research

Grifolic acid induces GH3 adenoma cell death by inhibiting ATP production through a GPR120-independent mechanism.
CONCLUSION: Grifolic acid induces GH3 cell death by decreasing MMP and inhibiting ATP production, which may be due to the inhibition of NADH production through a GPR120-independent mechanism. PMID: 29843779 [PubMed - in process]
Source: BMC Pharmacology and Toxicology - May 31, 2018 Category: Drugs & Pharmacology Tags: BMC Pharmacol Toxicol Source Type: research

Reversal of Multidrug Resistance in an Epirubicin-Resistant Gastric Cancer Cell Subline
Conclusions: ABCB1 knockdown decreased EPI efflux and increased EPI sensitivity in AGS/EPI cells. This result provides a novel strategy for targeted gene therapy to reverse EPI resistance in gastric cancer. PMID: 29801407 [PubMed - in process]
Source: Asian Pacific Journal of Cancer Prevention - May 31, 2018 Category: Cancer & Oncology Tags: Asian Pac J Cancer Prev Source Type: research

A hyaluronan-based nanosystem enables combined anti-inflammation of mTOR gene silencing and pharmacotherapy.
In this study, we synthesized a hyaluronan-based ROS-sensitive polymer which was expected to release loaded chemical drugs in inflammatory environment and further developed a stable and nontoxic co-delivery nanosystem of siRNA targeting autophagy suppressive gene and chemotherapeutic agents. The in vitro transfection study of this nanosystem revealed improved intracellular accumulation of siRNA and excellent gene silencing efficacy comparable to that of conventional cationic liposome. Moreover, the mRNA expression of inflammatory cytokines was remarkably decreased by our nanosystem. Considering its biocompatibility, transf...
Source: Atherosclerosis - May 31, 2018 Category: Cardiology Authors: Hou C, Bai H, Wang Z, Qiu Y, Kong LL, Sun F, Wang D, Yin H, Zhang X, Mu H, Duan J Tags: Carbohydr Polym Source Type: research

Chloride intracellular channel 1 as a switch among tumor behaviors in human esophageal squamous cell carcinoma.
Conclusions: The present results suggest that the very strong expression of CLIC1 enhances tumor survival, while its very weak expression promotes cellular movement. The present study provides an insight into the role of CLIC1 as a switch among tumor behaviors in ESCC. PMID: 29796185 [PubMed]
Source: Oncotarget - May 31, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

PKD1 is a potential biomarker and therapeutic target in triple-negative breast cancer.
Authors: Spasojevic C, Marangoni E, Vacher S, Assayag F, Meseure D, Château-Joubert S, Humbert M, Karam M, Ricort JM, Auclair C, Regairaz M, Bièche I Abstract Protein Kinase D1 (PKD1) is a serine/threonine kinase encoded by the PRKD1 gene. PKD1 has been previously shown to be a prognostic factor in ERα+ tamoxifen-resistant breast tumors and PKD1 overexpression confers estrogen independence to ERα+ MCF7 cells. In the present study, our goal was to determine whether PKD1 is a prognostic factor and/or a relevant therapeutic target in breast cancer. We analyzed PRKD1 mRNA levels in 527 primary breast tumors. We fou...
Source: Oncotarget - May 31, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1.
Authors: Schwartz M, Böckmann S, Borchert P, Hinz B Abstract Activation of the p38 mitogen-activated protein kinase (MAPK) pathway has been implicated in various detrimental events finally leading to endothelial dysfunction. The present study therefore investigates the impact of the p38 MAPK inhibitor SB202190 on the expression of the cytoprotective enzyme heme oxygenase-1 (HO-1) as well as metabolic activity, apoptosis and autophagy of endothelial cells. Using human umbilical vein endothelial cells (HUVEC) SB202190 was found to cause a time- and concentration-dependent induction of HO-1 protein. Induction of HO-1...
Source: Oncotarget - May 31, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Oncodriver inhibition and CD4+ Th1 cytokines cooperate through Stat1 activation to induce tumor senescence and apoptosis in HER2+ and triple negative breast cancer: implications for combining immune and targeted therapies.
Authors: Rosemblit C, Datta J, Lowenfeld L, Xu S, Basu A, Kodumudi K, Wiener D, Czerniecki BJ Abstract In patients with HER2-expressing breast cancer many develop resistance to HER2 targeted therapies. We show that high and intermediate HER2-expressing cancer cell lines are driven toward apoptosis and tumor senescence when treated with either CD4+ Th1 cells, or Th1 cytokines TNF-α and IFN-γ, in a dose dependent manner. Depletion of HER2 activity by either siRNA or trastuzumab and pertuzumab, and subsequent treatment with either anti-HER2 Th1 cells or TNF-α and IFN-γ resulted in synergistic increased tumor senes...
Source: Oncotarget - May 31, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Disturbed alternative splicing of FIR (PUF60) directed cyclin E overexpression in esophageal cancers.
In conclusion, elevated cyclin E expression was, in part, induced owing to potential FIR/FIRΔexon2-FBW7 interaction in ESCC. PMID: 29796163 [PubMed]
Source: Oncotarget - May 31, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

YdjC chitooligosaccharide deacetylase homolog induces keratin reorganization in lung cancer cells: involvement of interaction between YDJC and CDC16.
In this study, we investigated the role and molecular mechanisms of YdjC chitooligosaccha- ride deacetylase homolog (YDJC) in sphingosylphosphorylcholine (SPC)-induced phosphorylation and reorganization of K8, and migration and invasion (SPC-induced events). SPC induced expression of YDJC in a dose- and time-dependent manner. Gene silencing of YDJC suppressed SPC-induced events. YDJC overexpression induced the SPC-induced events. YDJC deacetylase dominant negative mutant (YDJCD13A) did not induce SPC-induced events. YDJC siRNA reduced ERK activation and overexpression of YDJC induced ERK activation. The siRNA of ERK1 or ER...
Source: Oncotarget - May 31, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

The Role of Palladin in Podocytes
Conclusions Palladin has an important role in podocyte function in vitro and in vivo.
Source: Journal of the American Society of Nephrology : JASN - May 31, 2018 Category: Urology & Nephrology Authors: Artelt, N., Ludwig, T. A., Rogge, H., Kavvadas, P., Siegerist, F., Blumenthal, A., van den Brandt, J., Otey, C. A., Bang, M.-L., Amann, K., Chadjichristos, C. E., Chatziantoniou, C., Endlich, K., Endlich, N. Tags: Basic Research Source Type: research

Co-inhibition of Pol η and ATR sensitizes cisplatin-resistant non-small cell lung cancer cells to cisplatin by impeding DNA damage repair.
In this study, we showed that there was no difference in intracellular uptake of cisplatin or the removal of platinum-DNA adducts between a cisplatin-resistant NSCLC cell line (A549/DR) and a cisplatin-sensitive NSCLC cell line (A549). However, the capacity to repair DNA interstrand crosslinks (ICLs) and double-strand breaks (DSBs) was significantly enhanced in the A549/DR cell line compared to 3 cisplatin-sensitive cell lines. We found that the protein and mRNA expression levels of Pol η, a Y-family translesion synthesis (TLS) polymerase, were markedly increased upon cisplatin exposure in A549/DR cells compared with A549...
Source: Acta Pharmacologica Sinica - May 31, 2018 Category: Drugs & Pharmacology Authors: Li XQ, Ren J, Chen P, Chen YJ, Wu M, Wu Y, Chen K, Li J Tags: Acta Pharmacol Sin Source Type: research

Targeting ALK in pediatric RMS does not induce antitumor activity in vivo
ConclusionsWhile ALK appears to be a relevant target in RMS in vitro, targeting this kinase in vivo yields no therapeutic efficacy, warranting extreme caution when considering the use of these agents in pediatric RMS patients.
Source: Cancer Chemotherapy and Pharmacology - May 31, 2018 Category: Cancer & Oncology Source Type: research

SERPINA3 Silencing Inhibits the Migration, Invasion, and Liver Metastasis of Colon Cancer Cells.
CONCLUSION: SERPINA3 silencing could inhibit the migration, invasion, and liver metastasis of colon cancer cells. PMID: 29855767 [PubMed - as supplied by publisher]
Source: Anal Sci - May 31, 2018 Category: Chemistry Authors: Cao LL, Pei XF, Qiao X, Yu J, Ye H, Xi CL, Wang PY, Gong ZL Tags: Dig Dis Sci Source Type: research

SPHK1 promotes metastasis of thyroid carcinoma through activation of the S1P/S1PR3/Notch signaling pathway.
In conclusion, the present study indicated that high levels of p-SPHK1 were positively correlated with high levels of S1P which in turn promoted thyroid carcinoma metastasis via the S1P/S1P receptor 3/Notch signaling pathway, suggesting possible prognostic markers and therapeutic targets. PMID: 29805524 [PubMed]
Source: Experimental and Therapeutic Medicine - May 30, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

The action mechanism of lncRNA-HOTAIR on the drug resistance of non-small cell lung cancer by regulating Wnt signaling pathway.
Authors: Guo F, Cao Z, Guo H, Li S Abstract The action mechanism of long non-coding ribonucleic acid-homeobox transcript antisense ribonucleic acid (lncRNA-HOTAIR) in the regulation of the Wnt signaling pathway on the drug resistance of non-small cell lung cancer was investigated. Forty eight patients with non-small cell lung cancer, who were treated with cisplatin (DDP) as neoadjuvant chemotherapy, were selected from the specimen bank of the Department of Pathology of Peking Union Medical College Hospital. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the messenger RNA (mRNA) level of...
Source: Experimental and Therapeutic Medicine - May 30, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Knockdown of SIRT1 inhibits proliferation and promotes apoptosis of paclitaxel-resistant human cervical cancer cells.
This study investigates the role of Sirtuin 1 (SIRT1) in paclitaxel (PTX)-resistant CC lines. We used qPCR and Western blots to measure SIRT1 mRNA and protein expressions in 10 matched clinical cancer tissues. We compared the expression levels of SIRT1 between sensitive CC cell lines and PTX-resistant cell lines. Subsequently, we used SIRT1 siRNA to knockdown the expression of SIRT1, and then measured cell proliferation, cell apoptosis rate, cell cycle distribution, and expression levels of Bcl-2 and Bax in PTX-sensitive Hela cell line, PTX-resistant Hela and Sila-resistant cell lines. Finally, we detected the location and...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Luteolin promotes the sensitivity of cisplatin in ovarian cancer by decreasing PRPA1-medicated autophagy.
In this study, luteolin was found to suppress autophagy with reduced expression of LC3-II, but enhanced the inhibition of cell vitality and promoted apoptosis induced by cisplatin, leading to restoration of the sensitivity to cisplatin in ovarian cancer cells through CCK-8, flow cytometry and immunofluorescent assays. Although cisplatin elevated the PARP1 for cell survival, the cisplatin-induced expression of PARP1 was inhibited by luteolin a dose- and time- dependent manner through Q-PCR and WB assays. Further, PARP1 siRNA could further improve the LUT-induced inhibition of cell vitality and restore the sensitivity to cis...
Source: Cellular and Molecular Biology - May 30, 2018 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

ASAP Hierarchical Assembly of siRNA with Tetraamino Fullerene in Physiological Conditions for Efficient Internalization into Cells and Knockdown
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b01869
Source: ACS Applied Materials and Interfaces - May 30, 2018 Category: Materials Science Authors: Kosuke Minami, Koji Okamoto, Koji Harano, Eisei Noiri, Eiichi Nakamura Source Type: research

Dexmedetomidine protects hepatic cells against oxygen-glucose deprivation/reperfusion injury via lncRNA CCAT1.
This study suggests a novel role of CCAT1 in ischemia reperfusion injury, and lays the framework for future studies. PMID: 29851220 [PubMed - as supplied by publisher]
Source: Cell Biology International - May 30, 2018 Category: Cytology Authors: Zhou Z, Chen Q, Wan L, Zheng D, Li Z, Wu Z Tags: Cell Biol Int Source Type: research

Application of cationized magnetoferritin for magnetic field-assisted delivery of short interfering RNA in vitro
AbstractCationized magnetoferritin is used for development of a simple, efficient, and fast delivery of short interference RNA into cells using combination of magnetophoresis for pre-concentration of siRNA-magnetoferritin complex on the surface of plated cells with subsequent application of nanosecond laser pulses producing stress waves in transfection chamber, which permeabilize cell membrane for the facilitated delivery of siRNA into the cell interior. As has been quantified using siRNA inducing cell death assay, by combination of these two physical factors we have obtained high efficiency for tested three different huma...
Source: Lasers in Medical Science - May 30, 2018 Category: Laser Surgery Source Type: research

β3-adrenergic receptor activation induces TGFβ1 expression in cardiomyocytes via the PKG/JNK/c-Jun pathway.
In conclusion, the β3-AR activation in cardiomyocytes increases the expression of TGFβ1 via the PKG/JNK/c-Jun pathway. These results help us further understand the role of β3-AR signaling in heart failure. PMID: 29859189 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - May 30, 2018 Category: Biochemistry Authors: Xu Z, Wu J, Xin J, Feng Y, Hu G, Shen J, Li M, Zhang Y, Xiao H, Wang L Tags: Biochem Biophys Res Commun Source Type: research

ASAP Theranostic Niosomes for Efficient siRNA/MicroRNA Delivery and Activatable Near-Infrared Fluorescent Tracking of Stem Cells
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b05513
Source: ACS Applied Materials and Interfaces - May 29, 2018 Category: Materials Science Authors: Chuanxu Yang, Shan Gao, Ping Song, Frederik Dagn æs-Hansen, Maria Jakobsen, Jørgen Kjems Source Type: research

S-adenosylhomocysteine Hydrolase Participates in DNA Methylation Inheritance
Publication date: Available online 28 May 2018 Source:Journal of Molecular Biology Author(s): V.K. Chaithanya Ponnaluri, Pierre-Olivier Estève, Cristian I. Ruse, Sriharsa Pradhan DNA (cytosine-5) methyltransferase 1 (DNMT1) is essential for mammalian development and maintenance of DNA methylation following DNA replication in cells. The DNA methylation process generates S-adenosyl-l-homocysteine, a strong inhibitor of DNMT1. Here we report that S-adenosylhomocysteine hydrolase (SAHH/AHCY), the only mammalian enzyme capable of hydrolyzing S-adenosyl-l-homocysteine binds to DNMT1 during DNA replication. SAHH enhances DNMT1 ...
Source: Journal of Molecular Biology - May 29, 2018 Category: Molecular Biology Source Type: research

A β1-42 Oligomer induces alteration of Tight Junction scaffold proteins via RAGE-mediated Autophagy in bEnd.3 cells.
Aβ1-42 Oligomer induces alteration of Tight Junction scaffold proteins via RAGE-mediated Autophagy in bEnd.3 cells. Exp Cell Res. 2018 May 29;: Authors: Chan Y, Chen W, Wan W, Chen Y, Li Y, Zhang C Abstract Compelling evidences have shown that amyloid-β (Aβ) peptide is one of the major pathogenic factors resulting in blood-brain barrier (BBB) disruption in Alzheimer's disease (AD). However, the mechanism underlying BBB breakdown remains elusive. In our present study, we employed murine brain capillary endothelial cells (bEnd.3) as an in vitro BBB model to investigate the role of autophagy in Aβ1-4...
Source: Experimental Cell Research - May 29, 2018 Category: Cytology Authors: Chan Y, Chen W, Wan W, Chen Y, Li Y, Zhang C Tags: Exp Cell Res Source Type: research

Glucose-regulated protein of 94 kDa contributes to the development of an aggressive phenotype in breast cancer cells
Publication date: September 2018 Source:Biomedicine & Pharmacotherapy, Volume 105 Author(s): Pedro Buc Calderon, Anne-Laure Sennesael, Christophe Glorieux Grp94 plays an essential role in protein assembly. We previously suggested that Grp94 overexpression is involved in tumor aggressiveness. However, the underlying mechanisms remain unknown. Since many tumors display high Grp94 levels, we investigated the effects of tumor microenvironment on the regulation of this chaperone expression. First, we found out that hypoxia did not change Grp94 expression in the human tumor cell lines MCF-7 (breast cancer) and HepG2 (li...
Source: Biomedicine and Pharmacotherapy - May 29, 2018 Category: Drugs & Pharmacology Source Type: research

Endothelial Replicative Senescence Delayed by the Inhibition of MTORC1 Signaling Involves MicroRNA-107.
This study sought to identify miRNAs involved in MTORC1-mediated inhibition of replicative senescence in endothelial cells. Pre-senescent HUVECs were prolonged treated with low dose rapamycin (1 nM), an MTOR inhibitor. Rapamycin treatment down-regulated the phosphorylated MTOR, RPS6 and 4EBP1 expressions, which confirmed MTORC1 suppression. Prolonged low dose rapamycin treatment has significantly reduced the percentage of senescence-associated beta galactosidase (SA-β gal) positively stained senescent cells and P16INK4 A expression in these cells. On the contrary, the percentage of BrdU-labelled proliferating cells ha...
Source: The International Journal of Biochemistry and Cell Biology - May 29, 2018 Category: Biochemistry Authors: Khor ES, Wong PF Tags: Int J Biochem Cell Biol Source Type: research

Bush sophora root polysaccharide could help prevent aflatoxin B1-induced hepatotoxicity in the primary chicken hepatocytes.
Abstract The aim of this study was to evaluate the effects of bush sophora root polysaccharide (BSRPS) on the aflatoxin B1 (AFB1)-induced hepatotoxicity and to explore the underlying mechanisms. The primary chicken hepatocytes were used as the model in the present experiment. The results showed that AFB1 induced hepatotoxicity of chicken hepatocytes in a dose dependent manner as demonstrated by decreasing cell viability and increasing LDH activity, ALT and AST levels. AFB1 at 0.16 μM significantly increased the levels of hepatic cytochrome P450 1A5 (CYP450 1A5) mRNA and malondialdehyde (MDA) and decreased the a...
Source: Toxicon - May 29, 2018 Category: Toxicology Authors: Gan F, Yang Y, Chen Y, Che C, Pan C, Huang K Tags: Toxicon Source Type: research

Role of autophagy in sevoflurane-induced neurotoxicity in neonatal rat hippocampal cells.
CONCLUSION: This study demonstrates that sevoflurane may induce hippocampal neuron autophagy in primary cultured hippocampal neuronal cell and that Beclin-1 and Atg5 are involved in the process of sevoflurane-induced autophagy. Exposure of sevoflurane may not only induce autophagy of hippocampal neurons but also activate the apoptosis of hippocampal neurons. Autophagy may play an important role in sevoflurane-induced neurotoxicity in primary cultured hippocampal neuronal cells. PMID: 29857124 [PubMed - as supplied by publisher]
Source: Brain Research Bulletin - May 29, 2018 Category: Neurology Authors: Xu L, Shen J, Yu L, Sun J, McQuillan PM, Hu Z, Yan M Tags: Brain Res Bull Source Type: research

miR-21 promotes cardiac fibroblast-to-myofibroblast transformation and myocardial fibrosis by targeting Jagged1.
This study aimed to investigate the role of miR-21 in the regulation of CMT and myocardial fibrosis. Primary rat CFs were isolated from young SD rats and treated with TGF-β1, miR-21 sponge or Jagged1 siRNA. Cell proliferation, invasion and adhesion were detected. MI model was established in male SD rats using LAD ligation method and infected with recombinant adenovirus. The heart function and morphology was evaluated by ultrasonic and histological analysis. We found that TGF-β1 induced the up-regulation of miR-21 and down-regulation of Jagged1 in rat CFs. Luciferase assay showed that miR-21 targeted 3'-UTR of Jagged1 in ...
Source: J Cell Mol Med - May 28, 2018 Category: Molecular Biology Authors: Zhou XL, Xu H, Liu ZB, Wu QC, Zhu RR, Liu JC Tags: J Cell Mol Med Source Type: research

RHCG suppresses cervical cancer progression through inhibiting migration and inducing apoptosis regulated by TGF- β1.
RHCG suppresses cervical cancer progression through inhibiting migration and inducing apoptosis regulated by TGF-β1. Biochem Biophys Res Commun. 2018 May 28;: Authors: Wang DG, Li TM, Liu X Abstract Cervical cancer is the second commonest cancer among women in the worldwide, and the majority cause of death in various countries, highlighting the importance of investigating new therapeutic targets. Rh family, C glycoprotein (RHCG) belongs to the Rhesus (Rh) family and was first identified as Rh blood group antigens. It has been confirmed to function in cancer progression, including prostate cancer and ...
Source: Biochemical and Biophysical Research communications - May 28, 2018 Category: Biochemistry Authors: Wang DG, Li TM, Liu X Tags: Biochem Biophys Res Commun Source Type: research

Interleukin-32 promotes detachment and activation of human Langerhans cells in a human skin explant model.
CONCLUSIONS: We propose, for the first time, that IL-32 is a molecular link between KCs and LCs in healthy skin, provoking LC migration from the epidermis to the dermis prior to their migration to the draining lymph nodes. PMID: 29806155 [PubMed - as supplied by publisher]
Source: The British Journal of Dermatology - May 28, 2018 Category: Dermatology Authors: Gonnet J, Perrin H, Hutton AJ, Boccara D, Bonduelle O, Mimoun M, Atlan M, Soria A, Combadière B Tags: Br J Dermatol Source Type: research

Eriocheir sinensis microRNA-7 targets crab Myd88 to enhance white spot syndrome virus replication
In this study, the function of microRNA-7 (miR-7) in host-virus interaction was investigated. Replication of White spot syndrome virus (WSSV) was enhanced with the overexpression of miR-7 and inhibited with the downregulation of miR-7 by using anti-miRNA oligonucleotide AMO-miR-7. The target gene of miR-7 was predicted using bioinformatics methods. Results showed that crab myeloid differentiation factor 88 (Myd88) could be targeted by miR-7. When the expression of Myd88 was knocked down by sequence-specific siRNA, WSSV copies in crabs were significantly increased. Further findings revealed that knockdown of Myd88, Tube, or...
Source: Fish and Shellfish Immunology - May 27, 2018 Category: Biology Source Type: research

Lethal (2) giant larvae regulates pleural mesothelial cell polarity in pleural fibrosis
In this study, we observed that PMC polarity was lost in fibrotic pleura. Next we found increased Lethal (2) giant larvae (Lgl) bound with aPKC and PAR-6B competing against PAR-3A in PAR complex, which led to cell polarity loss. Then we demonstrated that Lgl1 siRNA prevented cell polarity loss in PMCs, and Lgl1 conditional knockout (ER-Cre+/− Lgl1 flox/flox ) attenuated pleural fibrosis in a mouse model. Our data indicated that Lgl1 regulates cell polarity of PMCs, inhibition of Lgl1 and maintenance of cell polarity in PMCs could be a potential therapeutic treatment approach for pleural fibrosis.
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - May 27, 2018 Category: Molecular Biology Source Type: research

Regulation of NADPH oxidase NOX4 by delta iodolactone (IL- δ) in thyroid cancer cells
Conclusions The antiproliferative and pro-apoptotic effect of IL-δ is mediated by different mechanisms and pathway involving different sources of ROS generation depending on the cellular context. Graphical abstract
Source: Molecular and Cellular Endocrinology - May 27, 2018 Category: Endocrinology Source Type: research

Raddeanin A, a natural triterpenoid saponin compound, exerts anticancer effect on human osteosarcoma via the ROS/JNK and NF- κB signal pathway.
Raddeanin A, a natural triterpenoid saponin compound, exerts anticancer effect on human osteosarcoma via the ROS/JNK and NF-κB signal pathway. Toxicol Appl Pharmacol. 2018 May 27;: Authors: Ma B, Zhu J, Zhao A, Zhang J, Wang Y, Zhang H, Zhang L, Zhang Q Abstract Osteosarcoma (OS) is the most frequent and high mortality primary bone tumor in the adolescent. And it is well-known for poor prognosis due to high incidence of metastasis. Raddeanin A (RA), an active component of Anemone raddeana Regel, showed potential anti-cancer activities. However, the anti-tumor effect and molecular mechanism(s) of RA o...
Source: Toxicology and Applied Pharmacology - May 27, 2018 Category: Toxicology Authors: Ma B, Zhu J, Zhao A, Zhang J, Wang Y, Zhang H, Zhang L, Zhang Q Tags: Toxicol Appl Pharmacol Source Type: research

Lethal (2) giant larvae regulates pleural mesothelial cell polarity in pleural fibrosis.
In this study, we observed that PMC polarity was lost in fibrotic pleura. Next we found increased Lethal (2) giant larvae (Lgl) bound with aPKC and PAR-6B competing against PAR-3A in PAR complex, which led to cell polarity loss. Then we demonstrated that Lgl1 siRNA prevented cell polarity loss in PMCs, and Lgl1 conditional knockout (ER-Cre+/-Lgl1flox/flox) attenuated pleural fibrosis in a mouse model. Our data indicated that Lgl1 regulates cell polarity of PMCs, inhibition of Lgl1 and maintenance of cell polarity in PMCs could be a potential therapeutic treatment approach for pleural fibrosis. PMID: 29842893 [PubMed -...
Source: Biochimica et Biophysica Acta - May 26, 2018 Category: Biochemistry Authors: Song LJ, Zhou LL, Wang M, Liu F, Xiong L, Xiang F, Yu F, He XL, Xu JJ, Shi HZ, Xin JB, Ma WL, Ye H Tags: Biochim Biophys Acta Source Type: research

c-Abl phosphorylation of Yin Yang 1's conserved tyrosine 254 in the spacer region modulates its transcriptional activity
In conclusion, we demonstrate the novel role of c-Abl kinase in regulation of YY1's transcriptional activity, linking YY1 regulation with c-Abl tyrosine kinase signaling pathways.
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - May 26, 2018 Category: Molecular Biology Source Type: research

Genome characterization of Sugarcane Yellow Leaf Virus with special reference to RNAi based molecular breeding
Publication date: July 2018 Source:Microbial Pathogenesis, Volume 120 Author(s): Farghama Khalil, Xu Yueyu, Xiao Naiyan, Liu Di, Muhammad Tayyab, Wang Hengbo, Waqar Islam, Saeed Rauf, Chen Pinghua Sugarcane is an essential crop for sugar and biofuel. Globally, its production is severely affected by sugarcane yellow leaf disease (SCYLD) caused by Sugarcane Yellow Leaf Virus (SCYLV). Many aphid vectors are involved in the spread of the disease which reduced the effectiveness of cultural and chemical management. Empirical methods of plant breeding such as introgression from wild and cultivated germplasm were not possible or ...
Source: Microbial Pathogenesis - May 26, 2018 Category: Infectious Diseases Source Type: research

Cofilin Knockdown Attenuates Hemorrhagic Brain Injury-induced Oxidative Stress and Microglial Activation in Mice
In this study, we explored the effects of cofilin knockdown in a mouse model of ICH. Animals given stereotaxic injections of cofilin siRNA, 72-h prior to induction of ICH by collagenase injection within the area of siRNA administration showed significantly decreased cofilin expression levels and lower hemorrhage volume and edema, and the animals performed significantly better in neurobehavioral tasks i.e., rotarod, grip strength and neurologic deficit scores. Cofilin siRNA knocked-down mice had reduced ICH-induced DNA fragmentation, blood–brain barrier disruption and microglial activation, with a concomitant increase in ...
Source: Neuroscience - May 26, 2018 Category: Neuroscience Source Type: research

The surveillance of pre-mRNA splicing is an early step in C. elegans RNAi of endogenous genes Research Papers
We present biochemical evidence that RNAi targeted transcripts are tightly bound to spliceosomes. These findings suggest multiple layers of regulation by the spliceosome at early steps of small RNA-mediated gene silencing.
Source: Genes and Development - May 25, 2018 Category: Genetics & Stem Cells Authors: Newman, M. A., Ji, F., Fischer, S. E. J., Anselmo, A., Sadreyev, R. I., Ruvkun, G. Tags: Research Papers Source Type: research

PH domain leucine-rich repeat protein phosphatase 2 (PHLPP2) regulates G-protein-coupled receptor kinase 5 (GRK5)-induced cardiac hypertrophy in vitro Cell Biology
PH domain leucine-rich repeat protein phosphatase (PHLPP) is a serine/threonine phosphatase that has been shown to regulate cell growth and survival through dephosphorylation of several members of the AGC family of kinases. G-protein–coupled receptor kinase 5 (GRK5) is an AGC kinase that regulates phenylephrine (PE)-induced cardiac hypertrophy through its noncanonical function of directly targeting proteins to the nucleus to regulate transcription. Here we investigated the possibility that the PHLPP2 isoform can regulate GRK5-induced cardiomyocyte hypertrophy in neonatal rat ventricular myocytes (NRVMs). We show that rem...
Source: Journal of Biological Chemistry - May 25, 2018 Category: Chemistry Authors: Szu-Tsen Yeh, Cristina M. Zambrano, Walter J. Koch, Nicole H. Purcell Tags: Signal Transduction Source Type: research

Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells Cell Biology
Iron–sulfur (Fe-S) clusters are ancient cofactors in cells and participate in diverse biochemical functions, including electron transfer and enzymatic catalysis. Although cell lines derived from individuals carrying mutations in the Fe-S cluster biogenesis pathway or siRNA-mediated knockdown of the Fe-S assembly components provide excellent models for investigating Fe-S cluster formation in mammalian cells, these experimental strategies focus on the consequences of prolonged impairment of Fe-S assembly. Here, we constructed and expressed dominant–negative variants of the primary Fe-S biogenesis scaffold protein iron–...
Source: Journal of Biological Chemistry - May 25, 2018 Category: Chemistry Authors: Daniel R. Crooks, Nunziata Maio, Andrew N. Lane, Michal Jarnik, Richard M. Higashi, Ronald G. Haller, Ye Yang, Teresa W-M. Fan, W. Marston Linehan, Tracey A. Rouault Tags: Editors ' Picks Source Type: research

c-Abl phosphorylation of Yin Yang 1's conserved tyrosine 254 in the spacer region modulates its transcriptional activity.
In conclusion, we demonstrate the novel role of c-Abl kinase in regulation of YY1's transcriptional activity, linking YY1 regulation with c-Abl tyrosine kinase signaling pathways. PMID: 29807053 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - May 25, 2018 Category: Biochemistry Authors: Daraiseh SI, Kassardjian A, Alexander KE, Rizkallah R, Hurt MM Tags: Biochim Biophys Acta Source Type: research