Query: siRNA

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High circulatory leptin mediated NOX-2-peroxynitrite-miR21 axis activate mesangial cells and promotes renal inflammatory pathology in nonalcoholic fatty liver disease
Publication date: July 2018Source: Redox Biology, Volume 17Author(s): Firas Alhasson, Ratanesh Kumar Seth, Sutapa Sarkar, Diana A. Kimono, Muayad S. Albadrani, Diptadip Dattaroy, Varun Chandrashekaran, Geoffrey I. Scott, Samir Raychoudhury, Mitzi Nagarkatti, Prakash Nagarkatti, Anna Mae Diehl, Saurabh ChatterjeeAbstractHigh circulatory insulin and leptin followed by underlying inflammation are often ascribed to the ectopic manifestations in non-alcoholic fatty liver disease (NAFLD) but the exact molecular pathways remain unclear. We have shown previously that CYP2E1-mediated oxidative stress and circulating leptin in NAFLD...
Source: Redox Biology - July 11, 2018 Category: Biology Source Type: research

Neuralized1a regulates asymmetric division in mouse Lewis lung carcinoma cells
Publication date: 1 August 2018Source: Life Sciences, Volume 206Author(s): Liangsheng Kong, Jingyuan Li, Yongli Liu, Zhiwei Sun, Shixia Zhou, Junling Tang, Ting Ye, Jianyu Wang, H. Rosie XingAbstractAsymmetric division (ASD), the unique characteristic of normal stem cells, is regarded as a stemness marker when applied to the study of cancer stem cells (CSCs). However, the role of ASD in the self-renewal of CSCs and its regulation remain largely unknown. Here, we first established a mouse Lewis lung carcinoma CSC cell line that could undergo asymmetric division (LLC-ASD cells) derived from the parental mouse Lewis lung carc...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via the CIRP-MAPK signaling pathway in H9c2 cardiac cells
In this study, we hypothesize that exogenous H2S may protect cardiac cells against high glucose (HG)-induced myocardial injury and inflammation with the involvement of the CIRP-MAPK signaling pathway.Main methodsH9c2 cardiac cells cultured under HG conditions were transfected with siRNA and different inhibitors for detecting the effects of sodium hydrogen sulfide (NaHS) (a H2S donor) on cell biological processes. The cardiac cell viability and LDH activity were determined by CCK-8 and LDH kit. ELISA was employed to measure the levels of inflammatory factors, while 2′,7′-dichlorofluorescein diacetate (DCFH-DA) to evalua...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

CRIP1 promotes cell migration, invasion and epithelial-mesenchymal transition of cervical cancer by activating the Wnt/β‑catenin signaling pathway
In conclusion, CRIP1 promotes cell migration and invasion, mediates EMT and activates the Wnt/β‑catenin signaling pathway in CC.
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Fibulin-1 interacts with Sex Hormone Binding Globulin and is linked to less aggressive estrogen-dependent breast cancers
In this study we investigated the expression of fibulins, their functional relationship with SHBG and involvement in behavior of estrogen-sensitive breast cancer.Main methodsGene expression of fibulins was performed by Real time-PCR on two estrogen-sensitive breast cancer cell lines, MCF-7 and T47D. Fibulin-1 protein expression and localization were determined by Western blot and immunofluorescence. SHBG interaction with-fibulin-1 was assessed by GST-pull down assay. MCF-7 cell growth and gene expression, after fibulin-1 silencing by siRNA, were evaluated. Finally, the expression of fibulin-1 was correlated to clinical and...
Source: Life Sciences - July 11, 2018 Category: Biology Source Type: research

Daidzein-rich isoflavone aglycones inhibit cell growth and inflammation in endometriosis
In conclusion, DRIAs inhibit cellular proliferation in endometriosis, thus representing a potential therapeutic option for the management of endometriosis.
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Molecular mechanisms underlying progesterone-induced cytoplasmic retention of p27 in breast cancer cells
Publication date: Available online 27 June 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Hui-Chen Wang, Wen-Sen LeeAbstractIt has been reported that progesterone (P4) can contribute to the aggressiveness of human breast cancers through promoting cytoplasmic localization of p27 and stimulating proliferation. However, the molecular mechanisms underlying P4-induced cytoplasmic retention of p27 are still unclear. Here, we demonstrated that P4 (12.5–100 nM) concentration-dependently increased the number of T47D and MCF-7 cells. P4 (50 nM) also time-dependently increased the levels of p27 ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Impairment of the Gnα11-controlled expression of claudin-1 and MMP-9 and collective migration of human breast cancer MCF-7 cells by DHEAS
Publication date: Available online 21 April 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Neha Upmanyu, Ahmed Bulldan, Dimitrios Papadopoulos, Raimund Dietze, Viveka Nand Malviya, Georgios Scheiner-BobisAbstractAlthough dehydroepiandrosterone sulfate (DHEAS) constitutes the most abundant steroid in humans, in-depth investigations of its effects are rather scarce. We address here DHEAS effects on the estrogen receptor-positive metastatic human breast cancer cell line MCF-7. We focus on DHEAS-mediated signaling that might influence expression of claudin-1 and matrix metalloproteinase-9 (MMP-...
Source: The Journal of Steroid Biochemistry and Molecular Biology - July 11, 2018 Category: Biochemistry Source Type: research

Apoptosis signal regulating kinase-1 and NADPH oxidase mediate human amylin evoked redox stress and apoptosis in pancreatic beta-cells
Publication date: Available online 6 April 2018Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Sanghamitra Singh, Diti Chatterjee Bhowmick, Satyabrata Pany, Myungkuk Joe, Noor Zaghlula, Aleksandar M. JeremicAbstractMisfolded toxic human islet amyloid polypeptide or amylin (hA) and plasma membrane-associated redox complex, NADPH oxidase (NOX), have been implicated in the islet β-cell demise associated with type-2 diabetes mellitus (T2DM). Studies show that hA accumulation is stressful to β-cells and that misfolding of human amylin evokes redox stress and activates mitogen activated protein (MAP) kinas...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - July 11, 2018 Category: Biochemistry Source Type: research

Direct and specific inhibition of constitutive nitric oxide synthase uniquely regulates brush border membrane Na-absorptive pathways in intestinal epithelial cells
Publication date: 1 September 2018Source: Nitric Oxide, Volume 79Author(s): Balasubramanian Palaniappan, Uma SundaramAbstractPharmacological manipulations of constitutive nitric oxide (cNO) levels have been shown to have variable effects on Na absorption in vivo and in vitro in different tissues. Species differences, untoward in vivo effects (e.g. ENS, blood flow) and pharmacological non-specificity may account for these confounding observations. Thus, to directly and specifically determine the effect of cNO on brush border membrane Na/H exchange (NHE3) and Na-dependent glucose co-transport (SGLT-1), we inhibited cNO sy...
Source: Nitric Oxide - July 11, 2018 Category: Chemistry Source Type: research

Avobenzone suppresses proliferative activity of human trophoblast cells and induces apoptosis mediated by mitochondrial disruption
This study suggests that avobenzone induces mitochondrial dysfunction-mediated apoptosis leading to abnormal placentation during early pregnancy.
Source: Reproductive Toxicology - July 11, 2018 Category: Toxicology Source Type: research

Theabrownin triggers DNA damage to suppress human osteosarcoma U2OS cells by activating p53 signalling pathway.
Abstract Osteosarcoma becomes the second leading cause of cancer death in the younger population. Current outcomes of chemotherapy on osteosarcoma were unsatisfactory to date, demanding development of effective therapies. Tea is a commonly used beverage beneficial to human health. As a major component of tea, theabrownin has been reported to possess anti-cancer activity. To evaluate its anti-osteosarcoma effect, we established a xenograft model of zebrafish and employed U2OS cells for in vivo and in vitro assays. The animal data showed that TB significantly inhibited the tumour growth with stronger effect than t...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Jin W, Zhou L, Yan B, Yan L, Liu F, Tong P, Yu W, Dong X, Xie L, Zhang J, Xu Y, Li C, Yuan Q, Shan L, Efferth T Tags: J Cell Mol Med Source Type: research

Heat-shock transcription factor 1 is critically involved in the ischaemia-induced cardiac hypertrophy via JAK2/STAT3 pathway.
Abstract Cardiac hypertrophy after myocardial infarction (MI) is an independent risk factor for heart failure. Regression of cardiac hypertrophy has emerged as a promising strategy in the treatment of MI patients. Here, we have been suggested that heat-shock transcription factor 1 (HSF1) is a novel repressor of ischaemia-induced cardiac hypertrophy. Ligation of left anterior descending coronary was used to produce MI in HSF1-deficient heterozygote (KO), HSF1 transgenic (TG) mice and their wild-type (WT) littermates, respectively. Neonatal rat cardiomyocytes (NRCMs) were treated by hypoxia to mimic MI in vitro. The...
Source: J Cell Mol Med - July 11, 2018 Category: Molecular Biology Authors: Yuan L, Qiu L, Ye Y, Wu J, Wang S, Wang X, Zhou N, Zou Y Tags: J Cell Mol Med Source Type: research

Inhibition of the Nrf2/HO-1 Axis Suppresses the Mitochondria-Related Protection Promoted by Gastrodin in Human Neuroblastoma Cells Exposed to Paraquat
AbstractMitochondria are double-membrane organelles involved in the transduction of energy from different metabolic substrates into adenosine triphosphate (ATP) in mammalian cells. The oxidative phosphorylation system is comprised by the activity of the respiratory chain and the complex V (ATP synthase/ATPase). This system is dependent on oxygen gas (O2) in order to maintain a flux of electrons in the respiratory chain, since O2 is the final acceptor of these electrons. Electron leakage from this complex system leads to the continuous generation of reactive species in the cells. The mammalian cells exhibit certain mechanis...
Source: Molecular Neurobiology - July 11, 2018 Category: Neurology Source Type: research

Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference
Publication date: June 2018Source: Genes & Diseases, Volume 5, Issue 2Author(s): Shujuan Yan, Ruyi Zhang, Ke Wu, Jing Cui, Shifeng Huang, Xiaojuan Ji, Liping An, Chengfu Yuan, Cheng Gong, Linghuan Zhang, Wei Liu, Yixiao Feng, Bo Zhang, Zhengyu Dai, Yi Shen, Xi Wang, Wenping Luo, Bo Liu, Rex C. Haydon, Michael J. LeeAbstractMesenchymal stem cells (MSCs) are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic, chondrogenic and adipogenic lineages. We previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molec...
Source: Genes and Diseases - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Silencing of PRR11 suppresses cell proliferation and induces autophagy in NSCLC cells
Publication date: June 2018Source: Genes & Diseases, Volume 5, Issue 2Author(s): Lian Zhang, Yunlong Lei, Ying Zhang, Yi Li, Youquan Bu, Fangzhou Song, Chundong ZhangAbstractOur previous studies have demonstrated that proline-rich protein 11 (PRR11) is a novel tumor-related gene and implicates in regulating the proliferation in lung cancer. However, its precise role in cell cycle progression remains unclear. Our recent evidences show that PRR11 silencing has an effect on autophagy in non-small-cell lung cancer (NSCLC) cells. Two human NSCLC cell lines, H1299 and A549 were transiently transfected with against PRR11 siRNA. T...
Source: Genes and Diseases - July 10, 2018 Category: Genetics & Stem Cells Source Type: research

Role of PERK-eIF2α signaling pathway in fetal male rats with hypospadias induced by di-n-butyl phthalate
This study aims to explore the role of PERK-eIF2α signaling pathway in fetal male rats with hypospadias induced by maternal exposure to di-n-butyl phthalate (DBP). DBP was used to treat pregnant SD rats by gastric intubation from gestation day (GD) 14–18 to construct a hypospadias rat model. The amount, weight, anogenital distance (AGD), and hypospadias incidence of rats were recorded and the genital tubercle (GT) of fetal male rats was collected on GD 19. Western blotting was performed to detect the expressions of PERK-eIF2α pathway- and autophagy-related proteins, and cell apoptosis was detected using TUNEL method. T...
Source: The Kaohsiung Journal of Medical Sciences - July 10, 2018 Category: Universities & Medical Training Source Type: research

Methacholine induces extracellular matrix production by human airway smooth muscle cells through β-catenin signaling
This study attempted to investigate the role of methacholine in promoting ECM production by human ASMCs (HASMCs) and the underlying mechanism. We found that methacholine induced the expression of collagen I protein and multiple ECM genes. β-catenin signaling was activated in this process upon GSK3β phosphorylation, leading to upregulation of total and active β-catenin. Silencing β-catenin by specific small interfering RNA (siRNA) or with the β-catenin inhibitor, PKF115-584, decreased collagen I expression. Conversely, overexpression of active β-catenin by adenoviruses carrying the S33Y-β-catenin mutant increased the...
Source: Respiratory Physiology and Neurobiology - July 10, 2018 Category: Respiratory Medicine Source Type: research

Macrophage migration inhibitory factor is a novel prognostic marker for human oral squamous cell carcinoma
The objective of this study was to investigate the expression of MIF and its receptor CD74 in OSCC tissues, and to study the function of MIF in OSCC cells. Tissues of 90 patients with OSCC from the School of Stomatology, China Medical University were collected, and immunohistochemical staining and quantitative reverse transcription polymerase chain reaction were performed for MIF and CD74. The possible correlations between MIF and CD74 and clinical characteristics were analysed. The Kaplan-Meier analysis was used to determine the survival rates of patients. In addition, the proliferation and invasion of OSCC cells were eva...
Source: Pathology Research and Practice - July 10, 2018 Category: Pathology Source Type: research

Knockdown of TRIM66 inhibits malignant behavior and epithelial-mesenchymal transition in non-small cell lung cancer
ConclusionSilence TRIM66 expression suppressed NSCLC cell proliferation, invasion, and migration. The siRNA-mediated TRIM66 silencing could block the occurrence of EMT. TRIM66 could be a promising novel target for future NSCLC treatments.
Source: Pathology Research and Practice - July 10, 2018 Category: Pathology Source Type: research

IL-1β promotes the migration of olfactory epithelium neural stem cells through activating matrix metalloproteinase expressions
ConclusionOur study demonstrated that IL-1β promoted the migration of OENSCs through activating MMP expression. Moreover, JNK and NF-κB signaling pathways were involved in the regulation. This study provides important experimental evidence for the application of OENSCs in the transplantation therapy.
Source: Pathology Research and Practice - July 10, 2018 Category: Pathology Source Type: research

Beta2 adrenergic receptor silencing change intraocular pressure in New Zealand rabbits
ConclusionThe use of siRNA against the beta2 adrenergic receptors could provide an interesting therapeutic strategy for glaucoma treatment.ResumenObjetivoEl glaucoma consiste en un grupo de neuropatías ópticas progresivas caracterizadas por degeneración del nervio óptico y pérdida irreversible del campo visual. La elevación de la presión intraocular es el único factor de riesgo tratable probado, centrándose los productos comerciales para el tratamiento del glaucoma en la reducción de la presión intraocular. Estos fármacos pueden tener diversos efectos secundarios indeseados, lo cual invita a buscar nuevas estra...
Source: Journal of Optometry - July 10, 2018 Category: Opthalmology Source Type: research

Decidual ACVR2A regulates extravillous trophoblast functions of adhesion, proliferation, migration and invasion in vitro
Publication date: April 2018Source: Pregnancy Hypertension, Volume 12Author(s): Hannah E.J. Yong, Padma Murthi, Bill Kalionis, Rosemary J. Keogh, Shaun P. BrenneckeAbstractDecidual stromal cells form the largest proportion of maternal cells at the maternal-fetal interface. Our aim was to investigate the role of the pre-eclampsia associated decidual activin receptor, ACVR2A, in regulating trophoblast functions at this interface. St-T1b and HTR-8/SVneo cell lines were used to model decidual stromal and trophoblast cells respectively. St-T1b conditioned medium inhibited HTR-8/SVneo adhesion, proliferation, migration and invas...
Source: Pregnancy Hypertension: An International Journal of Womens Cardiovascular Health - July 10, 2018 Category: OBGYN Source Type: research

Reduced Methylation Downregulates CD39/ENTPD1 and ZDHHC14 to Suppress Trophoblast Cell Proliferation and Invasion: Implications in Preeclampsia
Publication date: Available online 24 March 2018Source: Pregnancy HypertensionAuthor(s): Lisha Zhu, Ruitu Lv, Lingchun Kong, Xiaotian LiAbstractPreeclampsia (PE) is a pregnancy-specific syndrome affecting up to 8% of pregnancies worldwide. While PE is a leading cause of maternal and neonatal mortality and morbidity, the pathophysiology of PE is unclear to date. Here, we have verified that dysregulation of CD39/ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1) and zinc finger DHHC-type containing 14 (ZDHHC14) via DNA methylation plays a vital role in late-onset PE pathology. Our study confirmed the differentially me...
Source: Pregnancy Hypertension: An International Journal of Womens Cardiovascular Health - July 10, 2018 Category: OBGYN Source Type: research

Dysfunction of pseudogene PGK1P2 is involved in preeclampsia by acting as a competing endogenous RNA of PGK1
ConclusionsWe proved that PGK1 and PGK1P2 are a pair of ceRNAs against miR-330-5p and they play a vital role in human decidualization by regulating angiogenesis and glycolysis metabolism. The deficiency of PGK1 and PGK1P2 in the decidua jeopardizes the decidualization process and subsequently might lead to the occurrence of PE. These findings may help in promoting novel predictive, diagnostic and prognostic biomarkers of PE in future.
Source: Pregnancy Hypertension: An International Journal of Womens Cardiovascular Health - July 10, 2018 Category: OBGYN Source Type: research

DNA binding protein HMGB1 secreted by activated microglia promotes the apoptosis of hippocampal neurons in diabetes complicated with OSA
Publication date: Available online 18 June 2018Source: Brain, Behavior, and ImmunityAuthor(s): Yu Shi, Xiangyu Guo, Jie Zhang, Hanchi Zhou, Bei Sun, Jing FengAbstractType 2 diabetes mellitus (T2DM) complicated with obstructive sleep apnea (OSA) may cause neuronal apoptosis and cognitive deficits, but the underlying mechanisms remain unclear. We aimed to determine the relationship between the activation of microglia and the apoptosis of hippocampal neurons, specifically in terms of high mobility group box-1 (HMGB1), after high glucose (HG) and intermittent hypoxia (IH) exposure. Diabetic KK-Ay mice and non-diabetic C57BL/6J...
Source: Brain, Behavior, and Immunity - July 10, 2018 Category: Neurology Source Type: research

BLT1-mediated O-GlcNAcylation is required for NOX2-dependent migration, exocytotic degranulation and IL-8 release of human mast cell induced by Trichomonas vaginalis-secreted LTB4
Publication date: June–July 2018Source: Microbes and Infection, Volume 20, Issue 6Author(s): Arim Min, Young Ah Lee, Kyeong Ah Kim, Myeong Heon ShinAbstractTrichomonas vaginalis is a sexually-transmitted protozoan parasite that causes vaginitis and cervicitis. Although mast cell activation is important for provoking tissue inflammation during infection with parasites, information regarding the signaling mechanisms in mast cell activation and T. vaginalis infection is limited. O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of serine and threonine residues that functions as a critical regulato...
Source: Microbes and Infection - July 10, 2018 Category: Infectious Diseases Source Type: research

Genome-wide profiling of adult human papillary and reticular fibroblasts identifies ACAN, Col XI α1, and PSG1 as general biomarkers of dermis ageing, and KANK4 as an exemplary effector of papillary fibroblast ageing, related to contractility
Publication date: Available online 18 June 2018Source: Mechanisms of Ageing and DevelopmentAuthor(s): Valérie Haydont, Véronique Neiveyans, Hélène Zucchi, Nicolas O. Fortunel, Daniel AsselineauAbstractDeciphering the characteristics of dermal fibroblasts is critical to further understand skin ageing. We have conducted a genome-wide transcriptomic characterization of papillary (Fp) and reticular (Fr) fibroblasts extracted from human skin samples corresponding to younger and older adult ages. From this screen, biomarkers suitable for the assessment of chronological ageing were identified, and extrapolated to the context ...
Source: Mechanisms of Ageing and Development - July 10, 2018 Category: Geriatrics Source Type: research

Effect of heme oxygenase-1 on the protection of ischemia reperfusion injury of bile duct in rats after liver transplantation
ConclusionOur study demonstrated that overexpression of HO-1 in donor liver can ameliorate the damage to bile duct and liver, and improved liver function, suggesting HO-1 might be a new therapeutic target in the treatment of IRI after liver transplantation.
Source: Clinics and Research in Hepatology and Gastroenterology - July 10, 2018 Category: Gastroenterology Source Type: research

Vitamin D suppresses macrophage infiltration by down-regulation of TREM-1 in diabetic nephropathy rats
This study intends to investigate the effect of active vitamin D (VD) on the expression of triggering receptor expressed on myeloid cells-1 (TREM-1) in the renal tissues of diabetic nephropathy (DN) rats and to explore the impact of TREM-1 on macrophage adhesion and migration. We find that the expressions of TREM-1 and CD68 protein are higher in DN rats compared with rats in the normal control group and that these changes are decreased in the DN + VD group. In vitro, the capacity for macrophage adhesion and migration and the expression of TREM-1 are increased under high-glucose conditions, but VD inhibits this progress....
Source: Molecular and Cellular Endocrinology - July 10, 2018 Category: Endocrinology Source Type: research

Oxytocin alters the morphology of hypothalamic neurons via the transcription factor myocyte enhancer factor 2A (MEF-2A)
In this study, we present evidence for three hypotheses: 1) OT leads to neurite retraction in hypothalamic neurons via the OT receptor (OTR) 2) The transcription factor MEF-2A is a central regulator of OT-induced neurite retraction, and 3) The MAPK pathway is critical for OT-induced MEF-2A activation.Incubation of rat hypothalamic H32 cells with 10 nM to 1 μM OT, vasopressin, and the specific OTR agonist TGOT, over the course of 12 h resulted in a time-dependent, significant retraction of neurites. In addition, the size of the nuclear compartment increased, whereas the overall cell size remained unchanged. OT trea...
Source: Molecular and Cellular Endocrinology - July 10, 2018 Category: Endocrinology Source Type: research

Estrogen receptor beta signaling inhibits PDGF induced human airway smooth muscle proliferation
In this study, we evaluated the effect of selective ERα and ERβ modulators on platelet-derived growth factor (PDGF)-stimulated ASM proliferation and the mechanisms involved. Asthmatic and non-asthmatic primary human ASM cells were treated with PDGF, 17β-estradiol, ERα-agonist and/or ERβ-agonist and/or G-protein-coupled estrogen receptor 30 (GPR30/GPER) agonist and proliferation was measured using MTT and CyQuant assays followed by cell cycle analysis. Transfection of small interfering RNA (siRNA) ERα and ERβ significantly altered the human ASM proliferation. The specificity of siRNA transfection was confirmed by Wes...
Source: Molecular and Cellular Endocrinology - July 10, 2018 Category: Endocrinology Source Type: research

Intestinal CART is a regulator of GIP and GLP-1 secretion and expression
Publication date: Available online 6 April 2018Source: Molecular and Cellular EndocrinologyAuthor(s): L. Shcherbina, A. Lindqvist, A.-H. Thorén Fischer, E. Ahlqvist, E. Zhang, S.E. Falkmer, E. Renström, J. Koffert, H. Honka, N. WierupAbstractImpaired incretin effect is a culprit in Type 2 Diabetes. Cocaine- and amphetamine-regulated transcript (CART) is a regulatory peptide controlling pancreatic islet hormone secretion and beta-cell survival. Here we studied the potential expression of CART in enteroendocrine cells and examined the role of CART as a regulator of incretin secretion and expression.CART expression was foun...
Source: Molecular and Cellular Endocrinology - July 10, 2018 Category: Endocrinology Source Type: research

Up-regulation of microRNA-93 inhibits TGF-β1-induced EMT and renal fibrogenesis by down-regulation of Orai1
In this study, our data showed that miR-93 was dramatically decreased in renal tissues of patients with DN and TGF-β1-stimulated HK2 cells. Moreover, the decreased level of miR-93 was closely associated with the increased expression of Orai1. Overexpression of miR-93 decreased Orai1 expression, and then suppressed TGF-β1-mediated EMT and fibrogenesis. Next, we predicted that the Orai1 was a potential target gene of miR-93, and demonstrated that miR-93 could directly target Orai1. SiRNA targeting Orai1 was sufficient to suppress TGF-β1-induced EMT and fibrogenesis in HK2 cells. Furthermore, Overexpression of Orai1 partia...
Source: Journal of Pharmacological Sciences - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Akebia saponin D alleviates hepatic steatosis through BNip3 induced mitophagy
Publication date: April 2018Source: Journal of Pharmacological Sciences, Volume 136, Issue 4Author(s): Li-li Gong, Song Yang, Wen Zhang, Fei-fei Han, Ya-li Lv, Zi-Rui Wan, He Liu, Yang-jie Jia, Ling-ling Xuan, Li-hong LiuAbstractAkebia Saponin D (ASD) is the most abundant constituent of the rhizome of Dipsacus asper Wall. The prior studies have shown that ASD alleviates hepatic steatosis targeted at the modulation of autophagy and exerts hepatoprotective effects through mitochondria. However, it is still unclear which signal transduction pathway that ASD increase autophagy and protect the mitochondria. The purpose of this ...
Source: Journal of Pharmacological Sciences - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Bakuchiol exhibits anti-metastasis activity through NF-κB cross-talk signaling with AR and ERβ in androgen-independent prostate cancer cells PC-3
Publication date: Available online 16 June 2017Source: Journal of Pharmacological SciencesAuthor(s): Lin Miao, Xiaoting Yun, Rui Tao, Yuefei Wang, Xiumei Gao, Guanwei Fan, Yan Zhu, Ting Cai, Zhipeng Zhu, Chunlin YanAbstractAndrogen-independent prostate cancer (PCa) is a developed tumor derived from the local androgen dependent PCa, which often affects elderly men. Psoralea corylifolia L, a traditional Chinese medicine, has been widely used for PCa treatment as an important part of a common prescription, while the mechanism remains unclear. Our study was aimed to investigate the tumor-inhibitory effect of its main component...
Source: Journal of Pharmacological Sciences - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Alleviative effect of fucoxanthin-containing extract from brown seaweed Laminaria japonica on renal tubular cell apoptosis through upregulating Na+/H+ exchanger NHE1 in chronic kidney disease mice
ConclusionWe demonstrated that LJE inhibits renal apoptosis via NHE1 upregulation. The anti-apoptotic effect of LJE also improves renal function in CKD mice. Therefore, fucoxanthin-containing brown seaweed may have a therapeutic potential for CKD patients.Graphical abstract
Source: Journal of Ethnopharmacology - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Lipidation of polyethylenimine-based polyplex increases serum stability of bioengineered RNAi agents and offers more consistent tumoral gene knockdown in vivo
Publication date: 25 August 2018Source: International Journal of Pharmaceutics, Volume 547, Issues 1–2Author(s): Qian-Yu Zhang, Pui Yan Ho, Mei-Juan Tu, Joseph L. Jilek, Qiu-Xia Chen, Su Zeng, Ai-Ming YuAbstractRecently we have established a novel approach to produce bioengineered noncoding RNA agents (BERAs) in living cells that carry target RNAi molecules (e.g., siRNA and miRNA) and thus act as “prodrugs”. Using GFP-siRNA-loaded BERA (BERA/GFP-siRNA) as a model molecule, this study was to define the in vitro and in vivo knockdown efficiency of BERAs delivered by liposome-polyethylenimine nanocomplex (lipopolyplex o...
Source: International Journal of Pharmaceutics - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Functionalized carbon nanotubes as emerging delivery system for the treatment of cancer
Publication date: Available online 8 July 2018Source: International Journal of PharmaceuticsAuthor(s): Shubhangi Mahajan, Abhimanyu Patharkar, Kaushik Kuche, Rahul Maheshwari, Pran Kishore Deb, Kiran Kalia, Rakesh K. TekadeAbstractIn recent time, carbon nanotubes (CNTs) have gained vital importance for pharmaceutical formulation scientist for delivering drugs and genes, owing to their excellent surface properties. For example, their aspect ratio is thought to be responsible for their excellent cell penetration aptitude; anisotropic conductivity/semi-conductivity along their axis is ideal for integration with nervous and mu...
Source: International Journal of Pharmaceutics - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Spray freeze drying of small nucleic acids as inhaled powder for pulmonary delivery
In this study, spray freeze drying was employed to prepare dry powder of small nucleic acids. Mannitol and herring sperm DNA were used as bulking agent and model of small nucleic acid therapeutics, respectively. Formulations containing different solute concentration and DNA concentration were produced. The scanning electron microscope (SEM) images showed that the porosity of the particles increased as the solute concentration decreased. Powders prepared with solute concentration of 5% w/v were found to maintain a balance between porosity and robustness. Increasing concentration of DNA improved the aerosol performance of th...
Source: Asian Journal of Pharmaceutical Sciences - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Magnesium isoglycyrrhizinate ameliorates liver fibrosis and hepatic stellate cell activation by regulating ferroptosis signaling pathway
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Miao Sui, Xiaofei Jiang, Jun Chen, Haiyan Yang, Yan ZhuAbstractFerroptosis is recently reported as a new mode of regulated cell death. It is triggered by disturbed redox homeostasis, overloaded iron and increased lipid peroxidation. Howerver, the role of ferroptosis in hepatic fibrosis remains obscure. In the current study, we attempted to investigate the effect of Magnesium isoglycyrrhizinate (MgIG) on ferroptosis in liver fibrosis, and to further clarify the possible mechanisms. Our data showed that MgIG treatment markedly attenuate...
Source: Biomedicine and Pharmacotherapy - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Role of high mobility group A1/nuclear factor-kappa B signaling in coronary microembolization-induced myocardial injury
ConclusionsHMGA1/NF-κB signaling is involved in CME-induced myocardial inflammation. Inhibition of HMGA1/NF-κB signaling attenuated the CME-induced myocardial injury and improved cardiac function, suggesting a new potential target for the prevention and treatment of CME-induced myocardial injury.Graphical abstract
Source: Biomedicine and Pharmacotherapy - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Dendrobine targeting JNK stress signaling to sensitize chemotoxicity of cisplatin against non-small cell lung cancer cells in vitro and in vivo
ConclusionsThe combination treatment showed enhanced anticancer activity toward non-small cell lung cancer cells without aggravating the cardiotoxic effects of cisplatin suggesting that the combination strategy deserves further investigation for human lung cancer treatment.Graphical Abstract
Source: Phytomedicine - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

HMQ-T-F5 (1-(4-(2-aminoquinazolin-7-yl)phenyl)-3-(2-bromo-5- (trifluoromethoxy)phenyl) thiourea) suppress proliferation and migration of human cervical HeLa cells via inhibiting Wnt/β-catenin signaling pathway
ConclusionOur findings demonstrated that F5 can target β-catenin potentially and is useful in the treatment of cervical cancer.Graphical abstract.
Source: Phytomedicine - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Rhein protects the myocardiac cells against hypoxia/reoxygention-induced injury by suppressing GSK3β activity
ConclusionRhein protects myocardial H9c2 cells against hypoxia/reoxygenation induced injury via AKT/ GSK3β/p38 pathway.Graphical abstract
Source: Phytomedicine - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

In vitro inhibitory properties of sesquiterpenes from Chloranthus serratus on cell motility via down-regulation of LIMK1 activation in human breast cancer
Conclusioncollectively, it was concluded that SERA exhibited significant inhibitory effects on breast cancer cells migration, and these effects of this sesquiterpene are due to its properties reducing the activation of LIM kinase 1.Graphical Abstract
Source: Phytomedicine - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

Ginsenoside Rb1 increases macrophage phagocytosis through p38 mitogen-activated protein kinase/Akt pathway
ConclusionThese results suggest that ginsenoside Rb1 enhances the phagocytic capacity of macrophages for bacteria via activation of the p38/Akt pathway. Rb1 may be a useful pharmacological adjuvant for the treatment of bacterial infections in clinically relevant conditions.
Source: Journal of Ginseng Research - July 10, 2018 Category: Complementary Medicine Source Type: research

a1AMPK protects against endothelial barrier dysfunction through regulation of Cx43 expression and HSP27 phosphorylation
ConclusionOur work identifies the regulation of Cx43 expression and HSP27 phosphorylation as potential protective responses underlying the beneficial action of AMPK against endothelial barrier dysfunction.
Source: Archives of Cardiovascular Diseases Supplements - July 10, 2018 Category: Cardiology Source Type: research

Connexin-43 expression is increased by the nerve growth factor (NGF) and contributes to pulmonary arterial altered reactivity in pulmonary hypertension
ConclusionsOur results show that NGF increases Cx43 expression in PA through activation of its TrkA receptor and a PI3K/ERK-dependent signalling pathway. This mechanism seems to participate in NGF-induced PA hyperreactivity and may thus contribute to PH pathophysiology.
Source: Archives of Cardiovascular Diseases Supplements - July 10, 2018 Category: Cardiology Source Type: research

Role of TGF-β co-receptor CD109 in extracellular matrix production of smooth muscle cells
Publication date: April 2018Source: Archives of Cardiovascular Diseases Supplements, Volume 10, Issue 2Author(s): Y. Li, H. Louis, J. Gao-Li, A. Parlakian, O. Agbulut, P. Lacolley, V. Regnault, Z. LiCD109 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein located on the plasma membrane and has no intracellular domain. It has been reported that it attenuates the TGF-β signaling in skin fibroblastes and cancer cells by competitive binding to TGF-β factors and promoting internalization and degradation of TGF-β receptors. We found less production of extracellular matrix (ECM) and higher expression of CD109 in the c...
Source: Archives of Cardiovascular Diseases Supplements - July 10, 2018 Category: Cardiology Source Type: research