Query: siRNA

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Small RNA responses of Culex mosquitoes and cell lines during acute and persistent virus infection.
In conclusion, Hsu cells generated Merida virus-derived piRNAs, but our data suggests that the major sRNA response of Culex cells and mosquitoes to WNV infection is the exogenous siRNA response. It is also evident that sRNA responses differ significantly between specific virus-mosquito combinations. Future work using additional Culex-borne viruses may further elucidate how virus-derived piRNAs are generated in Culex cells and what role they may play in controlling replication of different viruses. PMID: 30959110 [PubMed - as supplied by publisher]
Source: Insect Biochemistry and Molecular Biology - April 5, 2019 Category: Biochemistry Authors: Rückert C, Prasad AN, Garcia-Luna SM, Robison A, Grubaugh ND, Weger-Lucarelli J, Ebel GD Tags: Insect Biochem Mol Biol Source Type: research

The Functions of Non-coding RNAs in rRNA Regulation
Qi Yan1, Chengming Zhu1, Shouhong Guang1,2* and Xuezhu Feng1* 1Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, China 2CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Hefei, China Ribosomes are ribonucleoprotein machines that decode the genetic information embedded in mRNAs into polypeptides. Ribosome biogenesis is tightly coordinated and controlled from the transcription of pre-rRNAs to the assembly of ribosomes. Defects or disorders in rRNA production result in a number of human ri...
Source: Frontiers in Genetics - April 5, 2019 Category: Genetics & Stem Cells Source Type: research

Quercetin enhances the antitumor activity of trichostatin A through up-regulation of p300 protein expression in p53 null cancer cells.
Abstract In the present study, we investigated the p53-independent mechanism by which quercetin (Q) increased apoptosis in human lung cancer H1299 cells exposed to trichostatin A (TSA), a histone deacetylase inhibitor. We also investigated the role of Q in increasing the acetylation of histones H3 and H4 and the possible mechanism. Q at 5 μM significantly increased apoptosis by 88% in H1299 cells induced by TSA at 72 h. Q also significantly increased TSA-induced death receptor 5 (DR5) mRNA and protein expression as well as caspase-10/3 activities in H1299 cells. Transfection of DR5 siRNA into H1299 ce...
Source: Chemico-Biological Interactions - April 5, 2019 Category: Molecular Biology Authors: Chuang CH, Chan ST, Chen CH, Yeh SL Tags: Chem Biol Interact Source Type: research

FoxO6-mediated IL-1β induces hepatic insulin resistance and age-related inflammation via the TF/PAR2 pathway in aging and diabetic mice
Publication date: Available online 3 April 2019Source: Redox BiologyAuthor(s): Dae Hyun Kim, Bonggi Lee, Jaewon Lee, Mi Eun Kim, Jun Sik Lee, Jae Heun Chung, Byung Pal Yu, H. Henry Dong, Hae Young ChungAbstractFoxO has been proposed to play a role in the promotion of insulin resistance, and inflammation. FoxO is a pro-inflammatory transcription factor that is a key mediator of generation of inflammatory cytokines such as IL-1β in the liver. However, the detailed association of FoxO6 with insulin resistance and age-related inflammation has not been fully documented. Here, we showed that FoxO6 was elevated in the livers of ...
Source: Redox Biology - April 4, 2019 Category: Biology Source Type: research

IFITM3 knockdown reduces the expression of CCND1 and CDK4 and suppresses the growth of oral squamous cell carcinoma cells
ConclusionsFrom our data we conclude that IFITM3 is overexpressed in OSCC and may regulate the CCND1-CDK4/6-pRB axis to mediate OSCC cell growth.
Source: Cellular Oncology - April 4, 2019 Category: Cancer & Oncology Source Type: research

A Parkinson's disease gene, DJ-1, regulates anti-inflammatory roles of astrocytes through prostaglandin D2 synthase expression.
Abstract Dysfunctional regulation of inflammation may contribute to the progression of neurodegenerative diseases. The results of this study revealed that DJ-1, a Parkinson's disease (PD) gene, regulated expression of prostaglandin D2 synthase (PTGDS) and production of prostaglandin D2 (PGD2), by which DJ-1 enhanced anti-inflammatory function of astrocytes. In injured DJ-1 knockout (KO) brain, expression of tumor necrosis factor-alpha (TNF-α) was more increased, but that of anti-inflammatory heme oxygenase-1 (HO-1) was less increased compared with that in injured wild-type (WT) brain. Similarly, astrocyte-conditi...
Source: Neurobiology of Disease - April 4, 2019 Category: Neurology Authors: Choi DJ, An J, Jou I, Park SM, Joe EH Tags: Neurobiol Dis Source Type: research

Mammalian STE20-like Kinase 1 Knockdown Attenuates TNF α-Mediated Neurodegenerative Disease by Repressing the JNK Pathway and Mitochondrial Stress.
Mammalian STE20-like Kinase 1 Knockdown Attenuates TNFα-Mediated Neurodegenerative Disease by Repressing the JNK Pathway and Mitochondrial Stress. Neurochem Res. 2019 Apr 04;: Authors: Geng C, Wei J, Wu C Abstract Neuroinflammation has been acknowledged as a primary factor contributing to the pathogenesis of neurodegenerative disease. However, the molecular mechanism underlying inflammation stress-mediated neuronal dysfunction is not fully understood. The aim of our study was to explore the influence of mammalian STE20-like kinase 1 (Mst1) in neuroinflammation using TNFα and CATH.a cells in vitro. T...
Source: Neurochemical Research - April 4, 2019 Category: Neuroscience Authors: Geng C, Wei J, Wu C Tags: Neurochem Res Source Type: research

Molecular targets for tivantinib (ARQ 197) and vasculogenic mimicry in human melanoma cells.
Abstract Tivantinib (TivB) was reported previously to target MET and microtubule assembly in different cells resulting in cytotoxicity. However, its other cellular targets remain unknown, especially the proteins involved in focal adhesion and cytoskeletal organization. We studied the effect of TivB on vinculin a focal adhesion protein, and RhoC, a GTPase which promote the reorganization of cytoskeleton. Biomolecules involved in vasculogenic mimicry (VM) previously not reported in melanoma, and their susceptibility to TivB was also evaluated. TivB affects the viability and apoptosis of human melanoma cells dependin...
Source: European Journal of Pharmacology - April 4, 2019 Category: Drugs & Pharmacology Authors: Kumar SR, Gajagowni S, Bryan JN, Bodenhausen HM Tags: Eur J Pharmacol Source Type: research

HMGB1 enhances AGE-mediated VSMC proliferation via an increase in 5-LO-linked RAGE expression.
Abstract Receptors for advanced glycation end-product (RAGE) play a pivotal role in the progression of proliferative vascular diseases. However, the precise mechanisms regulating RAGE expression in vascular smooth muscle cells (VSMCs) of the injured vasculatures is unclear. Given the potential importance of 5-lipoxygenase (5-LO) derived mediators in cellular responses mediated by RAGE, this study aimed to evaluate in VSMCs treated with high mobility group box 1 (HMGB1): 1) the RAGE expression; 2) the AGE-induced VSMC proliferation; 3) the role of 5-LO signaling in HMGB1-induced RAGE expression. In cultured human V...
Source: Vascular Pharmacology - April 4, 2019 Category: Drugs & Pharmacology Authors: Jang EJ, Baek SE, Kim EJ, Park SY, Kim CD Tags: Vascul Pharmacol Source Type: research

Dissecting PARP inhibitor resistance with functional genomics.
Abstract The poly-(ADP-ribose) polymerase (PARP) inhibitor (PARPi) olaparib was the first licenced cancer drug that targeted an inherited form of cancer, namely ovarian cancers caused by germline BRCA1 or BRCA2 gene mutations. Multiple different PARPi have now been approved for use in a wider group of gynaecological cancers as well as for the treatment of BRCA-gene mutant breast cancer. Despite these advances, resistance to PARPi is a common clinical phenotype. Understanding, at the molecular level, how tumour cells respond to PARPi has the potential to inform how these drugs should be used clinically and since th...
Source: Current Opinion in Genetics and Development - April 4, 2019 Category: Genetics & Stem Cells Authors: Pettitt SJ, Lord CJ Tags: Curr Opin Genet Dev Source Type: research

The co-chaperone UNC45A is essential for the expression of mitotic kinase NEK7 and tumorigenesis Gene Regulation
Cumulative evidence suggests that the heat shock protein 90 (Hsp90) co-chaperone UNC-45 myosin chaperone A (UNC45A) contributes to tumorigenesis and that its expression in cancer cells correlates with proliferation and metastasis of solid tumors. However, the molecular mechanism by which UNC45A regulates cancer cell proliferation remains largely unknown. Here, using siRNA-mediated gene silencing and various human cells, we report that UNC45A is essential for breast cancer cell growth, but is dispensable for normal cell proliferation. Immunofluorescence microscopy, along with gene microarray and RT-quantitative PCR analyses...
Source: Journal of Biological Chemistry - April 4, 2019 Category: Chemistry Authors: Nada H. Eisa, Yasmeen Jilani, Kashish Kainth, Priscilla Redd, Su Lu, Oulia Bougrine, Houssein Abdul Sater, Chaitanya A. Patwardhan, Austin Shull, Huidong Shi, Kebin Liu, Nehal M. Elsherbiny, Laila A. Eissa, Mamdouh M. El-Shishtawy, Anatolij Horuzsko, Roni Tags: Cell Biology Source Type: research

Induction of sestrin 2 is associated with fisetin-mediated apoptosis in human head and neck cancer cell lines.
In this study, the influence of fisetin on the growth and apoptosis of HNCCs were examined. In HSC3 cells, fisetin treatment reduced the viability and induced apoptosis. Through the results from the screening of the expression profile of apoptosis-related genes, sestrin 2 (SESN2) was functionally involved in fisetin-mediated apoptosis showing the knockdown of SESN2 by siRNA clearly restored fisetin-induced apoptosis. In addition, fisetin reduced the protein expression levels of phospho-mTOR (p-mTOR) and Mcl-1, which are the downstream molecules of SESN2. It also induced PARP cleavage by inducing an increase in the expressi...
Source: Journal of Clinical Biochemistry and Nutrition - April 3, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research

A non-autonomous role of MKL1 in the activation of hepatic stellate cells
In conclusion, our data suggest that MKL1 contribute to HSC activation in a non-autonomous fashion by promoting CTGF transcription in hepatocytes.
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - April 3, 2019 Category: Genetics & Stem Cells Source Type: research

IL-17A and IL-17F repair HIV-1 gp140 damaged Caco-2 cell barriers by upregulating tight junction genes
Publication date: Available online 2 April 2019Source: Microbes and InfectionAuthor(s): Fengjie Wang, Yanfang Cui, Xiuli Shen, Shuhui Wang, Gui-bo YangAbstractIt is widely accepted that impairment of the intestinal epithelial barrier from HIV/AIDS contributes significantly to microbial translocation and systemic immune activation. Such factors present potential targets for novel treatments aimed toward a functional cure. However, the extracellular mechanisms of intestinal barrier repair are poorly understood. In the current study, we investigated the abilities of IL-17A and IL-17F to repair the damaged barrier caused by HI...
Source: Microbes and Infection - April 3, 2019 Category: Infectious Diseases Source Type: research

Ventilator-induced lung injury is alleviated by inhibiting NLRP3 inflammasome activation
ConclusionsInhibiting NLRP3 inflammasome activation ameliorated VILI, suggesting a potential therapeutic target for the clinical treatment of VILI.
Source: Molecular Immunology - April 3, 2019 Category: Allergy & Immunology Source Type: research

Bronchial Epithelial Cells Promote the Differentiation of Th2 Lymphocytes in Airway Microenvironment through Jagged/Notch-1 Signaling after RSV Infection
Conclusion: Our data showed that BECs had the potential to promote the differentiation of Th2 lymphocytes through Jagged-1/Notch-1 signaling.Int Arch Allergy Immunol
Source: International Archives of Allergy and Immunology - April 3, 2019 Category: Allergy & Immunology Source Type: research

Regulation of P-glycoprotein by Bajijiasu in vitro and in vivo by activating the Nrf2-mediated signalling pathway.
CONCLUSIONS: BJJS activates Nrf2 to induce P-gp expression, and enhanced the efflux activity of P-gp. The possibility of potential herb-drug interactions when BJJS is co-administered with other P-gp substrate drugs should be carefully monitored. PMID: 30929555 [PubMed - in process]
Source: Pharmaceutical Biology - April 3, 2019 Category: Drugs & Pharmacology Tags: Pharm Biol Source Type: research

GSE101082 Effect of CXCR4 knockdown in CAFs on gene expression
Contributor : Kith PradhanSeries Type : Expression profiling by arrayOrganism : Homo sapiensCancer associated fibroblasts were used for siRNA mediated knockdown of CXCR4. Control and knockdown CAFs were used to generate RNA and cDNA that was used for gene expression analysis
Source: GEO: Gene Expression Omnibus - April 3, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Prenylated Xanthones from Metaxya rostrata suppress FoxM1 and induce active cell death by distinct mechanisms
ConclusionIn summary, our data clearly showed that XB and OH-XB are promising new lead compounds for cancer therapy with distinct cellular mechanisms. Both compounds are candidates for further pre-clinical and clinical investigations.Graphical abstract
Source: Phytomedicine - April 3, 2019 Category: Drugs & Pharmacology Source Type: research

Cancers, Vol. 11, Pages 466: Proteomic Analysis Implicates Vimentin in Glioblastoma Cell Migration
Lawler We previously showed lithium chloride (LiCl) and other inhibitors of glycogen synthase kinase-3 (GSK-3) including 6-bromo-indirubin-3-oxime (BIO), can block glioblastoma (GBM) cell migration. To investigate the mechanisms involved we used two-dimensional difference in-gel electrophoresis (2D-DIGE) and mass spectrometry to identify proteins altered after treatment of U251 GBM cells with 20 mM LiCl. Downregulation of the intermediate filament protein vimentin was the most significant change identified. Analysis of patient tumor samples revealed that vimentin is expressed abundantly in GBM, and is prognostic espec...
Source: Cancers - April 3, 2019 Category: Cancer & Oncology Authors: Michal O. Nowicki Josie L. Hayes E. Antonio Chiocca Sean E. Lawler Tags: Article Source Type: research

Concise approach for screening long non-coding RNAs functionally linked to human breast cancer associated genes.
In conclusion, this study provides a better understanding about the biological roles of lncRNAs in breast cancer and may be useful in the investigation of proper targets for diagnostic and/or therapeutic breast cancer markers using public databases. PMID: 30953646 [PubMed - as supplied by publisher]
Source: Experimental and Molecular Pathology - April 3, 2019 Category: Pathology Authors: Shin TJ, Lee KH, Cho HM, Cho JY Tags: Exp Mol Pathol Source Type: research

Loss of reticulocalbin 2 lowers blood pressure and restrains angiotensin II-induced hypertension in vivo.
Abstract Hypertension affects over one billion people worldwide and increases risk for heart failure, stroke and chronic kidney disease. Despite high prevalence and devastating impact, its etiology still remains poorly understood for most hypertensive cases. Rcn2, encoding reticulocalbin 2, is a candidate gene for atherosclerosis we previously demonstrated in mice. Here we identified Rcn2 as a novel regulator of blood pressure in mice. Rcn2 was dramatically upregulated in arteries undergoing structural remodeling. Deletion of Rcn2 lowered basal blood pressure and attenuated angiotensin II-induced hypertension in C...
Source: Am J Physiol Renal P... - April 3, 2019 Category: Urology & Nephrology Authors: Li J, Cechova S, Wang L, Isakson BE, Le T, Shi W Tags: Am J Physiol Renal Physiol Source Type: research

microRNA-33a prevents epithelial-mesenchymal transition, invasion and metastasis of gastric cancer cells through the Snail/Slug pathway.
This study suggests that miR-33a inhibited EMT, invasion and metastasis of GC through the Snail/Slug signaling pathway by modulating SNAI2 expression. PMID: 30943047 [PubMed - as supplied by publisher]
Source: American Journal of Physiology. Gastrointestinal and Liver Physiology - April 3, 2019 Category: Physiology Authors: Chen DD, Cheng JT, Chandoo A, Sun XW, Zhang L, Lu MD, Sun WJ, Huang YP Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research

Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines.
In conclusion, the results of the present study indicated that SIRT1 was positively associated with viability and invasion of U87 cells, potentially through EMT. These results suggested that SIRT1 may serve a crucial role in the proliferation and development of glioma. PMID: 30930981 [PubMed]
Source: Oncology Letters - April 2, 2019 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Inhibition of lysine-specific demethylase LSD1 induces senescence in Glioblastoma cells through a HIF-1α-dependent pathway
Publication date: Available online 2 April 2019Source: Biochimica et Biophysica Acta (BBA) - Gene Regulatory MechanismsAuthor(s): Carmen D. Saccà, Francesca Gorini, Susanna Ambrosio, Stefano Amente, Deriggio Faicchia, Giuseppe Matarese, Luigi Lania, Barbara MajelloAbstractSenescence is a stress-responsive cellular program that leads to cell cycle arrest. In cancer cells, senescence has profound implications for tumor aggressiveness and clinical outcome, but the molecular events that provoke cancer cells to undergo senescence remain unclear. Herein, we provide evidence that the histone demethylase LSD1/KDM1A supports the g...
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - April 2, 2019 Category: Genetics & Stem Cells Source Type: research

The effects of IFITM1 and IFITM3 gene deletion on IFN γ stimulated protein synthesis.
The effects of IFITM1 and IFITM3 gene deletion on IFNγ stimulated protein synthesis. Cell Signal. 2019 Apr 02;: Authors: Herranz MG, Nekulova M, Faktor J, Hernychova L, Kote S, Sinclair EH, Nenutil R, Vojtesek B, Ball KL, Hupp TR Abstract Interferon-induced transmembrane proteins IFITM1 and IFITM3 (IFITM1/3) play a role in both RNA viral restriction and in human cancer progression. Using immunohistochemical staining of FFPE tissue, we identified subgroups of cervical cancer patients where IFITM1/3 protein expression is inversely related to metastasis. Guide RNA-CAS9 methods were used to develop an is...
Source: Cellular Signalling - April 2, 2019 Category: Cytology Authors: Herranz MG, Nekulova M, Faktor J, Hernychova L, Kote S, Sinclair EH, Nenutil R, Vojtesek B, Ball KL, Hupp TR Tags: Cell Signal Source Type: research

Kr üppel-like factor 4 plays a role in the luteal transition in steroidogenesis by down-regulating Cyp19A1 expression.
Krüppel-like factor 4 plays a role in the luteal transition in steroidogenesis by down-regulating Cyp19A1 expression. Am J Physiol Endocrinol Metab. 2019 Apr 02;: Authors: Choi H, Ryu KY, Roh J Abstract The transition from granulosa cell to luteal cell involves a change from estrogen production to predominantly progesterone production. We analyzed the role of Krüppel-like factor 4 (Klf4), a transcriptional repressor used to generate pluripotent cells, in that transition. After luteinizing hormone/human chorionic gonadotropin (LH/hCG) treatment of preovulatory follicles, a major but transient increas...
Source: American Journal of Physiology. Endocrinology and Metabolism - April 2, 2019 Category: Physiology Authors: Choi H, Ryu KY, Roh J Tags: Am J Physiol Endocrinol Metab Source Type: research

4-Methylcoumarin-5,6-g-hesperetin attenuates inflammatory responses in alcoholic hepatitis through PPAR- γ activation.
In this study, we detected the anti-inflammatory activity of 4-MCH in EtOH fed mice and examined the potential molecular mechanism of this activity. We found that 4-MCH suppressed the release of inflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in primary liver macrophages isolated from mice and in EtOH-treated RAW264.7 cells. In addition, we showed that the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) was down-regulated in vivo and in vitro in AH. Furthermore, 4-MCH acted as an activator of PPAR-γ, which could therefore ameliorate the inhibitory effects of ...
Source: Toxicology - April 2, 2019 Category: Toxicology Authors: Meng HW, You HM, Yang Y, Zhang YL, Meng XM, Ma TT, Huang C, Li J Tags: Toxicology Source Type: research

5 ′-Morpholino modification of the sense strand of an siRNA makes it a more effective passenger
Chem. Commun., 2019, Accepted Manuscript DOI: 10.1039/C9CC00977A, CommunicationMuthiah Manoharan, Pawan Kumar, Rubina G. Parmar, Christopher R. Brown, Jennifer L. S. Willoughby, Donald Foster, Ramesh I Babu, Sally Schofield, Vasant Jadhav, Klaus Charisse, Jayaprakash K Nair, Kallanthottathil G Rajeev, Martin Maier, Martin Egli The 5 ’-monophosphate group plays an important role in strand selection during gene silencing mediated by small-interfering RNA. We show that blocking of 5′ phosphorylation of the sense strand by introducing... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - April 1, 2019 Category: Chemistry Authors: Muthiah Manoharan Source Type: research

Calcineurin B1 subunit in human peripheral blood mononuclear cells and its role in idiopathic membranous nephropathy
The immune responses involved in the pathogenesis of idiopathic membranous nephropathy (IMN) have not been fully understood. Calcineurin, a key signaling enzyme in T-cell activation, may be implicated in IMN. The present study aimed to investigate the role of calcineurin B1 subunit (CnB1) in IMN and the potential mechanism. A total of 59 biopsy-proven IMN patients and 28 healthy controls were recruited. The CnB1 expression in human peripheral blood mononuclear cells (PBMCs) was assessed by Western blotting. Knockdown and overexpression of CnB1 in Jurkat T cell line were achieved by small interference RNA (siRNA) transfect...
Source: Medicine - April 1, 2019 Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research

Expression of polymeric immunoglobulin receptor and its biological function in endometrial adenocarcinoma
Conclusion: PIgR may be a predictive biomarker of endometrial adenocarcinoma and a potential target protein for immunotherapy of endometrial adenocarcinoma.
Source: Journal of Cancer Research and Therapeutics - April 1, 2019 Category: Cancer & Oncology Authors: Mingshu Zhou Chongdong Liu Guangming Cao Huiqiao Gao Zhenyu Zhang Source Type: research

Salidroside attenuates oxidized low ‑density lipoprotein‑induced endothelial cell injury via promotion of the AMPK/SIRT1 pathway.
In conclusion, these results suggested that salidroside protects HUVECs against ox‑LDL injury through inhibiting oxidative stress and improving mitochondrial dysfunction, which were dependent on activating the AMPK/SIRT1 pathway. PMID: 30942428 [PubMed - as supplied by publisher]
Source: International Journal of Molecular Medicine - April 1, 2019 Category: Molecular Biology Authors: Zhao D, Sun X, Lv S, Sun M, Guo H, Zhai Y, Wang Z, Dai P, Zheng L, Ye M, Wang X Tags: Int J Mol Med Source Type: research

Loss of the Golgi Matrix Protein 130 Cause Aberrant IgA1 Glycosylation in IgA Nephropathy
Conclusion: We identified and demonstrated that GM130 plays an important role in IgA1 O-glycans deficiency in IgAN patients, by negatively  regulating C1GALT1 expression. We believe that this finding will provide theoretical foundations for a new mechanism of Gd-IgA1 production in IgAN patients.Am J Nephrol 2019;49:307 –316
Source: American Journal of Nephrology - March 31, 2019 Category: Neurology Source Type: research

GSE122641 Diap3 (Dia2) knock-down in murine marrow-derived mesenchymal stem cells
Contributors : Amel Dudakovic ; Andre J van WijnenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusControl (non-targeting) and Dia2-targeting siRNA were transfected into murine-derived mesenchymal stem cells and mRNA-Seq analysis was performed two days after transfection.
Source: GEO: Gene Expression Omnibus - March 31, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

Involvement of lncR-30245 in Myocardial Infarction–Induced Cardiac Fibrosis Through Peroxisome Proliferator-Activated Receptor-γ–Mediated Connective Tissue Growth Factor Signalling Pathway
ConclusionOur study indicates that the lncR-30245/PPAR-γ/CTGF pathway mediates MI-induced cardiac fibrosis and might be a therapeutic target for various cardiac diseases associated with fibrosis.RésuméContexteLes longs ARN non codants (lncRNA) se révèlent actuellement comme des médiateurs importants de la physiopathologie cardiaque. Le but de la présente étude est d’examiner les effets d’un lncRNA (lncR-30245) sur la fibrogenèse cardiaque et les mécanismes sous-jacents.MéthodologieUn infarctus du myocarde (IM) et le facteur de croissance transformant β1 (TGF-β1) ont été utilisés pour produire des phéno...
Source: Canadian Journal of Cardiology - March 30, 2019 Category: Cardiology Source Type: research

GSE129043 Differentially expressed genes after siRNA transfection in human cancer cell lines II
Contributors : Naohiko Seki ; Yasutaka YamadaSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo identify differentially expressed genes by anti cancer treatments (miRNA and siRNAs) in human cancer, several cell lines (breast cancer, colorectal cancer and renal cancer) were subjected to Agilent whole genome microarrays.
Source: GEO: Gene Expression Omnibus - March 30, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Identification of PEPT2 as an important candidate molecule in 5-ALA-mediated fluorescence-guided surgery in WHO grade II/III gliomas
ConclusionsThe results suggest PEPT2 is an important candidate molecule in 5-ALA-mediated FGS in grade II/III gliomas. As the overexpression of PEPT2 was associated with higher PpIX fluorescence intensity, PEPT2 may improve fluorescence-guided resection in grade II/III gliomas.
Source: Journal of Neuro-Oncology - March 30, 2019 Category: Cancer & Oncology Source Type: research

Euxanthone Ameliorates Sevoflurane-Induced Neurotoxicity in Neonatal Mice
This study was designed to investigate whether euxanthone can confer neuroprotective activities against sevoflurane-induced neurotoxicity and to determine the associated molecular mechanisms. Neonatal Sprague-Dawley (male) rats were exposed to sevoflurane with or without euxanthone treatment. The behavioral data of rats were collected at P41 (the beginning of the adult stage). The hippocampal tissue was obtained following exposure to sevoflurane. The reactive oxygen species (ROS) level in the hippocampal tissue was determined by a commercial kit. The expression of apoptotic markers and inflammatory cytokines was determined...
Source: Journal of Molecular Neuroscience - March 30, 2019 Category: Neuroscience Source Type: research

Apoptosis signal-regulating kinase 1 activation by Nox1-derived oxidants is required for TNF α receptor endocytosis.
Apoptosis signal-regulating kinase 1 activation by Nox1-derived oxidants is required for TNFα receptor endocytosis. Am J Physiol Heart Circ Physiol. 2019 Mar 29;: Authors: Choi H, Stark RJ, Raja BS, Dikalova A, Lamb FS Abstract Tumor necrosis factor-α (TNFα) is pro-inflammatory cytokine that is closely linked to the development of cardiovascular disease. TNFα activates NADPH oxidase 1 (Nox1), and reactive oxygen species (ROS) including superoxide (O2•-) production extracellularly are required for subsequent signaling in vascular smooth muscle cells (VSMC). Apoptosis signal-regulating kinase 1 (A...
Source: American Journal of Physiology. Heart and Circulatory Physiology - March 29, 2019 Category: Physiology Authors: Choi H, Stark RJ, Raja BS, Dikalova A, Lamb FS Tags: Am J Physiol Heart Circ Physiol Source Type: research

Suppression of long non-coding RNA RMRP ameliorates oxygen-glucose deprivation/re-oxygenation-induced neural cells injury by inhibiting autophagy and PI3K/Akt/mTOR-mediated apoptosis.
Abstract The aberrant expression of long non-coding RNAs (lncRNAs) has been inferred to be closely related with the progression of neural ischemia/reperfusion (I/R) injury. RMRP is an lncRNA associated with I/R injury. In order to determine the role of RMRP in I/R injury, the effects of RMRP knockdown on oxygen-glucose deprivation /reoxygenation (OGD/R)-induced injury in SH-SY5Y cells were evaluated. The effect of OGD/R administration on the expression of RMRP and apoptosis in SH-SY5Y cells, and the effect of RMRP suppression by siRNA on the impairments of cells proliferation and mobility potential due to OGD/R ad...
Source: Bioscience Reports - March 29, 2019 Category: Biomedical Science Authors: Zhou Z, Xu H, Liu B, Dun L, Lu C, Cai Y, Wang H Tags: Biosci Rep Source Type: research

Forkhead box O1 in turbot Scophthalmus maximus: Molecular characterization, gene structure, tissue distribution and the role in glucose metabolism.
In conclusion, data from both in vitro (primary hepatocytes) and in vivo (feeding trial) showed that downregulated foxo1 in turbot could not result in significant depression of gluconeogenesis and activation of glycolysis. This could be one of the reasons why intake of high level of carbohydrate resulted in prolonged hyperglycemia in turbot. PMID: 30935922 [PubMed - as supplied by publisher]
Source: Gene - March 29, 2019 Category: Genetics & Stem Cells Authors: Pan M, Zhang Y, Deng K, Liu G, Gu Z, Liu J, Luo K, Zhang W, Mai K Tags: Gene Source Type: research

The Matrix Metalloproteinase ‐13 Inhibitor Poricoic Acid ZI Ameliorates Renal Fibrosis by Mitigating Epithelial‐Mesenchymal Transition
ConclusionsThe findings indicate that as a specific MMP ‐13 inhibitor, PZI attenuates EMT and renal fibrosis. Therefore, the MMP‐13 inhibitor PZI could be a novel therapeutic candidate for limiting EMT and renal fibrosis.This article is protected by copyright. All rights reserved
Source: Molecular Nutrition and Food Research - March 29, 2019 Category: Food Science Authors: Lin Chen, Gang Cao, Ming Wang, Ya ‐Long Feng, Dan‐Qian Chen, Nosratola D. Vaziri, Shougang Zhuang, Ying‐Yong Zhao Tags: Research Article Source Type: research

Luteolin Ameliorates Testis Injury and Blood ‐Testis Barrier Disruption through the Nrf2 Signaling Pathway and by Up‐Regulating Cx43
Conclusion: Luteolin was identified as a novel active ingredient that contributed to the protective activity in testicular damage through activating the Nrf2 signaling pathway and by up ‐regulating Cx43.This article is protected by copyright. All rights reserved
Source: Molecular Nutrition and Food Research - March 29, 2019 Category: Food Science Authors: Bo Ma, Jie Zhang, Zhiming Zhu, Ang Zhao, Yanfen Zhou, Hanjie Ying, Qi Zhang Tags: Research Article Source Type: research

Pancreatic {beta}-cells detoxify H2O2 through the peroxiredoxin/thioredoxin antioxidant system Metabolism
Oxidative stress is thought to promote pancreatic β-cell dysfunction and contribute to both type 1 and type 2 diabetes. Reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, are mediators of oxidative stress that arise largely from electron leakage during oxidative phosphorylation. Reports that β-cells express low levels of antioxidant enzymes, including catalase and GSH peroxidases, have supported a model in which β-cells are ill-equipped to detoxify ROS. This hypothesis seems at odds with the essential role of β-cells in the control of metabolic homeostasis and organismal survival through exquisite...
Source: Journal of Biological Chemistry - March 29, 2019 Category: Chemistry Authors: Jennifer S. Stancill, Katarzyna A. Broniowska, Bryndon J. Oleson, Aaron Naatz, John A. Corbett Tags: Signal Transduction Source Type: research

The orphan nuclear receptor Nor1/Nr4a3 is a negative regulator of {beta}-cell mass Molecular Bases of Disease
The Nr4a subfamily of nuclear receptor comprises three members in mammalian cells: Nur77/Nr4a1, Nurr1/Nr4a2, and Nor1/Nr4a3. Nr4a proteins play key roles in the regulation of glucose homeostasis in peripheral metabolic tissues. However, their biological functions in β-cells remain relatively uncharacterized. Here we sought to investigate the potential role of Nor1 in the regulation of β-cell mass and, in particular, β-cell survival/apoptosis. We used histological analysis to examine the consequences of genetic deletion of either Nur77 and Nor1 on β-cell mass, investigated the expression patterns of Nr4as in human islet...
Source: Journal of Biological Chemistry - March 29, 2019 Category: Chemistry Authors: Anne–Francoise Close, Nidheesh Dadheech, Barbara Scoralick Villela, Claude Rouillard, Jean Buteau Tags: Molecular Bases of Disease Source Type: research

Viruses, Vol. 11, Pages 307: Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB)
s P. Gladue Classical swine fever virus (CSFV) E2 protein, the major virus structural glycoprotein, is an essential component of the viral envelope. E2 is involved in virus absorption, induction of a protective immune response and is critical for virulence in swine. Using the yeast two-hybrid system, we identified protein phosphatase 1 catalytic subunit beta (PPP1CB), which is part of the Protein Phosphatase 1 (PP1) complex, as a specific binding host partner for E2. We further confirmed the occurrence of this interaction in CSFV-infected swine cells by using two independent methodologies: Co-immunoprecipitation and Pr...
Source: Viruses - March 29, 2019 Category: Virology Authors: Elizabeth A. Vuono Elizabeth Ramirez-Medina Lauren G. Holinka Ryan Baker-Branstetter Manuel V. Borca Douglas P. Gladue Tags: Article Source Type: research

The antiviral protein viperin regulates chondrogenic differentiation via CXCL10 protein secretion Cell Biology
Viperin (also known as radical SAM domain–containing 2 (RSAD2)) is an interferon-inducible and evolutionary conserved protein that participates in the cell's innate immune response against a number of viruses. Viperin mRNA is a substrate for endoribonucleolytic cleavage by RNase mitochondrial RNA processing (MRP) and mutations in the RNase MRP small nucleolar RNA (snoRNA) subunit of the RNase MRP complex cause cartilage-hair hypoplasia (CHH), a human developmental condition characterized by metaphyseal chondrodysplasia and severe dwarfism. It is unknown how CHH-pathogenic mutations in RNase MRP snoRNA interfere with skel...
Source: Journal of Biological Chemistry - March 29, 2019 Category: Chemistry Authors: Mandy M. F. Steinbusch, Marjolein M. J. Caron, Don A. M. Surtel, Guus G. H. van den Akker, Paul J. van Dijk, Franziska Friedrich, Bernhard Zabel, Lodewijk W. van Rhijn, Mandy J. Peffers, Tim J. M. Welting Tags: Developmental Biology Source Type: research

Cancers, Vol. 11, Pages 446: Ion Channel Expression in Human Melanoma Samples: In Silico Identification and Experimental Validation of Molecular Targets
In conclusion, the present study identified five ion channels that can potentially serve as sensitive and specific markers in human melanoma specimens and demonstrates that the antifungal drug miconazole, known to target one of the five identified ion channels, exerts strong and specific anti-melanoma effects in vitro.
Source: Cancers - March 29, 2019 Category: Cancer & Oncology Authors: Daniela D ’Arcangelo Francesca Scatozza Claudia Giampietri Paolo Marchetti Francesco Facchiano Antonio Facchiano Tags: Article Source Type: research

Apelin-13 attenuates early brain injury following subarachnoid hemorrhage via suppressing neuronal apoptosis through the GLP-1R/PI3K/Akt signaling.
Abstract Apelin, an endogenous ligand for the orphan G-protein-coupled receptor APJ, possesses anti-apoptotic and neuroprotective properties. It has been shown to be a protective factor for different types of central nervous system insults, such as ischemia and traumatic brain injury. Here, we investigated the effects of apelin-13 on early brain injury (EBI) following subarachnoid hemorrhage (SAH), and the underlying molecular mechanisms involved. Apelin-13 was delivered to rats via intracerebroventricular administration. Neurological scores, brain water content and neuronal apoptosis were measured 24 h after SA...
Source: Biochemical and Biophysical Research communications - March 29, 2019 Category: Biochemistry Authors: Liu Y, Zhang T, Wang Y, Wu P, Li Y, Wang C, Xu S, Shi H Tags: Biochem Biophys Res Commun Source Type: research

Metformin alleviates inflammatory response in non-alcoholic steatohepatitis by restraining signal transducer and activator of transcription 3-mediated autophagy inhibition in  vitro and in vivo.
In conclusion, this study demonstrated that metformin alleviated the inflammatory response in the liver and the hepatocyte of the NASH model via STAT3-mediated autophagy induction. This mechanism provides a strategy for targeting the NASH inflammatory response. PMID: 30935688 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - March 29, 2019 Category: Biochemistry Authors: Li YL, Li XQ, Wang YD, Shen C, Zhao CY Tags: Biochem Biophys Res Commun Source Type: research