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Periprosthetic hypoxia as consequence of TRPM7 mediated cobalt influx in osteoblasts
This study shows that chemical hypoxia originating from HIF‐1α upregulation within the periprosthetic tissue is related to cobalt wear debris and highlights TRPM7 as an important key mediator in this context. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 00B: 0 00–000, 2018.
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - December 3, 2018 Category: Materials Science Authors: Constantin R ömmelt, Thomas Munsch, Andreas Drynda, Volkmar Lessmann, Christoph H. Lohmann, Jessica Bertrand Tags: Original Research Reports Source Type: research
Induction of migration of periodontal ligament cells by selective regulation of integrin subunits.
Abstract The recruitment of tissue-resident stem cells is important for wound regeneration. Periodontal ligament cells (PDL cells) are heterogeneous cell populations with stemness features that migrate into wound sites to regenerate periodontal fibres and neighbouring hard tissues. Cell migration is regulated by the local microenvironment, co-ordinated by growth factors and the extracellular matrix (ECM). Integrin-mediated cell adhesion to the ECM provides essential signals for migration. We have been suggested that PDL cell migration could be enhanced by selective expression of integrins. The migration of primary...
Source: J Cell Mol Med - December 3, 2018 Category: Molecular Biology Authors: Kawamura M, Yamamoto T, Yamashiro K, Kochi S, Yoshihara-Hirata C, Ideguchi H, Aoyagi H, Omori K, Takashiba S Tags: J Cell Mol Med Source Type: research
Effect of atorvastatin on cardiomyocyte hypertrophy through suppressing MURC induced by volume overload and cyclic stretch.
Abstract MURC (muscle-restricted coiled-coil protein) is a hypertrophy-related gene. Hypertrophy can be induced by mechanical stress. The purpose of this research was to investigate the hypothesis that MURC mediates hypertrophy in cardiomyocytes under mechanical stress. We used the in vivo model of an aortocaval shunt (AV shunt) in adult Wistar rats to induce myocardial hypertrophy. We also used the in vitro model of cyclic stretch in rat neonatal cardiomyocytes to clarify MURC expression and the molecular regulation mechanism. The flexible membrane culture plate seeding with cardiomyocytes Cardiomyocytes seeded o...
Source: J Cell Mol Med - December 3, 2018 Category: Molecular Biology Authors: Cheng WP, Lo HM, Wang BW, Chua SK, Shyu KG Tags: J Cell Mol Med Source Type: research
The AIB1siRNA-loaded hyaluronic acid-assembled PEI/heparin/Ca2+ nanocomplex as a novel therapeutic strategy in lung cancer treatment.
Abstract In the present study, AIB1siRNA‑loaded polyethyleneimine (PEI)/heparin/Ca2+ nanoparticles (NPs) were successfully prepared and evaluated for their efficacy in lung cancer cells. The results demonstrated that the PEI and heparin complex reduced the toxic effect in cancer cells while maintaining its transfection efficiency. A nanosized particle of ~25 nm was formulated and siRNA was demonstrated to possess excellent binding efficiency in the particles. Confocal microscopy revealed that fluorescein‑labeled (FAM)‑small interfering (si)RNA dissociated from the HA‑PEI/heparin/Ca2+/siRNA (CPH‑siH) NPs...
Source: International Journal of Molecular Medicine - December 3, 2018 Category: Molecular Biology Authors: Hao Y, Gao Y, Wu Y, An C Tags: Int J Mol Med Source Type: research
Downregulation of RTN1-C attenuates MPP+-induced neuronal injury through inhibition of mGluR5 pathway in SN4741 cells.
In conclusion, our data suggest that downregulation of RTN1-C protects SN4741 cells against MPP+ through mGluR5-mediated preservation of Ca2+ homeostasis. Therefore, RTN1-C might represent a therapeutic target for the treatment of neuronal injury in experimental PD models. PMID: 30521940 [PubMed - as supplied by publisher]
Source: Brain Research Bulletin - December 3, 2018 Category: Neurology Authors: Chang J, Zhang XL, Yu H, Chen J Tags: Brain Res Bull Source Type: research
Carboxypeptidase E- ΔN promotes migration, invasion and epithelial-mesenchymal transition of human osteosarcoma cell lines through the Wnt/β-catenin pathway.
In this study, we investigated the effect of CPE-ΔN on cell migration, invasion and epithelial-mesenchymal transition (EMT) of OS cells, and illustrated the molecular mechanisms. We first constructed CPE-ΔN overexpressing human OS cell lines (143B and U2OS cells), and found that ectopic CPE-ΔN expression in OS cells enhanced cellular migratory and invasiveness abilities, and promoted EMT process. Further, overexpression of CPE-ΔN increased c-myc and nuclear β-catenin levels in OS cells, which suggested CPE-ΔN promoted the activation of Wnt/β-catenin pathway in OS cells. Treatment with β-catenin small interfering RN...
Source: Biochemistry and Cell Biology - December 3, 2018 Category: Biochemistry Authors: Fan S, Gao X, Chen P, Li X Tags: Biochem Cell Biol Source Type: research
Cellular prion protein regulates the differentiation and function of adipocytes through autophagy flux
In this study, we investigated whether normal cellular prion protein (PrP<C>) is involved in the modulation of autophagy and affects adipogenic differentiation in vivo and in vitro. Surprisingly, autophagy flux signals were activated in the adipose tissue of prion protein-deficient mice and PrP<C>-deleted 3T3-L1 adipocytes. The activation of autophagy flux mediated by PrP<C> deletion was confirmed in the adipose tissue via transmission electron microscopy. Adipocyte differentiation factors were highly induced in prion protein-deficient adipose tissue and 3T3-L1 adipocytes. In addition, deletion of prion protein...
Source: Molecular and Cellular Endocrinology - December 2, 2018 Category: Endocrinology Source Type: research
Leukocyte ‑associated immunoglobulin‑like receptor 1 promotes tumorigenesis in RCC.
Leukocyte‑associated immunoglobulin‑like receptor 1 promotes tumorigenesis in RCC. Oncol Rep. 2018 Nov 20;: Authors: Jingushi K, Uemura M, Nakano K, Hayashi Y, Wang C, Ishizuya Y, Yamamoto Y, Hayashi T, Kinouchi T, Matsuzaki K, Kato T, Kawashima A, Ujike T, Nagahara A, Fujita K, Ueda K, Tsujikawa K, Nonomura N Abstract Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults, responsible for approximately 90‑95% of cases. We previously reported a novel method that enables direct extraction of extracellular vesicles (EVs) from surgically resected viable tissues, yielding what...
Source: Oncology Reports - December 1, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Molecular mechanism of LKB1 in the invasion and metastasis of colorectal cancer.
Authors: Chen Y, Liu Y, Zhou Y, You H Abstract The occurrence of colorectal cancer (CRC) is associated with a variety of oncogenes and tumor‑suppressor genes. As a tumor‑suppressor gene, the liver kinase B1 gene (LKB1, also known as serine/threonine kinase 11, STK11) is closely related to tumor angiogenesis, invasion and metastasis, but its molecular mechanisms remain unclear. The aim of the present study was to investigate the effects of LKB1 on the invasion and metastasis of CRC, and to explore its molecular mechanisms. By detecting the expression of LKB1 in CRC, we can provide a reference index for diagnosin...
Source: Oncology Reports - December 1, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Inhibition of lactate dehydrogenase A suppresses inflammatory response in RAW 264.7 macrophages.
Authors: Song YJ, Kim A, Kim GT, Yu HY, Lee ES, Park MJ, Kim YJ, Shim SM, Park TS Abstract Lactate is an important metabolite in cellular metabolism and fluctuates in certain disease conditions including cancer and immune diseases. It was hypothesized that a decrease in lactate would modulate the inflammatory response elicited by lipopolysaccharides (LPS) in macrophages. When RAW 264.7 macrophages were treated with FX11, a specific lactate dehydrogenase (LDHA) inhibitor, the expression of the cytokines, inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX‑2) was downregulated due to reduced cellular l...
Source: Molecular Medicine Reports - December 1, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Down-regulation of microRNA-216a confers protection against yttrium aluminium garnet laser-induced retinal injury via the GDNF-mediated GDNF/GFR α1/RET signalling pathway.
This study aims to elucidate the role of miR-216a in yttrium aluminium garnet (YAG) laser-induced retinal injury by targeting glial cell line-derived neurotrophic factor (GDNF) via GDNF/GDNF family neurotrophic factor receptor α1 (GFRα1)/rearranged during transfection (RET) signalling pathway. Wistar male rats were first randomly assigned into control and model groups. Immunohistochemistry was performed to detect the GDNF positive expression rate and terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) staining for apoptotic index (AI) of retinal tissue. Retinal neurons were divided into normal,...
Source: Journal of Biosciences - December 1, 2018 Category: Biomedical Science Authors: Hu XB, Fu SH, Luo QI, He JZ, Qiu YF, Lai W, Zhong M Tags: J Biosci Source Type: research
MiR-92a inhibits fibroblast-like synoviocyte proliferation and migration in rheumatoid arthritis by targeting AKT2.
This study was aimed to investigate the effects of miR-92a on the proliferation and migration of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs). Our results showed that miR-92a was significantly down-regulated in RA synovial tissue and RA-FLSs, whereas the protein level of AKT2 is increased. Restoration of miR-92a suppressed the proliferation and migration of RA-FLSs. Down-regulation of miR-92a promotes proliferation and migration of normal human FLSs. Dual luciferase reporter gene assay showed that miR-92a could specifically bind with the 30UTR of AKT2 and significantly repressed the luciferase activity. Down...
Source: Journal of Biosciences - December 1, 2018 Category: Biomedical Science Authors: Yu FY, Xie CQ, Jiang CL, Sun JT, Feng HC, Li C, Huang XW Tags: J Biosci Source Type: research
Cytokines promote lipolysis in 3T3-L1 adipocytes through induction of NADPH oxidase 3 expression and superoxide production Research Articles
NADPH oxidase (NOX) enzymes are one of the major superoxide-generating systems in cells. NOX-generated superoxide has been suggested to promote insulin resistance in the liver. However, the role of NOX enzymes in mediating metabolic dysfunction in other insulin target tissues remains unclear. Here, we show that NOX3 expression is induced in differentiated 3T3-L1 adipocytes upon treatment with proinflammatory cytokines. Superoxide production increased concurrently with NOX3 protein expression in cytokine-treated adipocytes, which was inhibited by the NOX inhibitor diphenyleneiodonium (DPI). Treatment of adipocytes with cyto...
Source: The Journal of Lipid Research - December 1, 2018 Category: Lipidology Authors: Issa, N., Lachance, G., Bellmann, K., Laplante, M., Stadler, K., Marette, A. Tags: Research Articles Source Type: research
Biodistribution and Toxicity Assessment of Superparamagnetic Iron Oxide Nanoparticles In Vitro and In Vivo
This study aimed to examine the toxicity and biodistribution of SPION coated with polyethylenimine (PEI) (SPION-PEI) designed for small interfering RNA (siRNA) delivery bothin vitro andin vivo. SPION-PEI/siRNA complexes were prepared at different weight ratios. Cytotoxic effects of SPION-PEI/siRNA on HSC-T6 cell viability were determined by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT). Rats were divided into three groups: a control group, a normal-saline group and a SPION-PEI/siRNA group. After a single intravenous injection,in vivo nanoparticle biodistribution and accumulation were evaluated ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2018 Category: Research Source Type: research
Myofibril Assembly in Cultured Mouse Neonatal Cardiomyocytes.
Abstract The formation of myofibrils was analyzed in neonatal mouse cardiomyocytes grown in culture and stained with fluorescent antibodies directed against myofibrillar proteins. The cardiomyocyte cultures also were exposed to siRNA probes to test the role of nonmuscle myosin IIB expression in the formation of myofibrils. In culture, new myofibrils formed in the spreading cell margins surrounding contractile myofibrils previously assembled in utero. Observations indicated that assembly of mature myofibrils occurred in three-stages, as previously reported in cultured mouse skeletal muscle. Premyofibrils, character...
Source: Anatomical Record - December 1, 2018 Category: Anatomy Authors: White J, Wang J, Fan Y, Dube DK, Sanger JW, Sanger JM Tags: Anat Rec (Hoboken) Source Type: research
Resolvin D1, but not resolvin E1, transactivates the epidermal growth factor receptor to increase intracellular calcium and glycoconjugate secretion in rat and human conjunctival goblet cells.
CONCLUSIONS: We conclude that in cultured rat and human conjunctival goblet cells, RvD1 activates the EGFR, increases [Ca2+]i, activates AKT and ERK1/2 to stimulate mucin secretion. RvE1 does not transactivate the EGFR to increase [Ca2+]I and stimulate mucin secretion, but does interact with the receptor to increase ERK 1/2 activity. PMID: 30513286 [PubMed - as supplied by publisher]
Source: Experimental Eye Research - December 1, 2018 Category: Opthalmology Authors: Kaye R, Botten N, Lippestad M, Li D, Hodges RR, Utheim TP, Serhan CN, Dartt DA Tags: Exp Eye Res Source Type: research
HtrA1 suppresses the growth of pancreatic cancer cells by modulating Notch-1 expression.
This study aimed to explore the function of HtrA1 in pancreatic cancer cell growth and its underlying mechanism. We found that the expression of HtrA1 was lower in pancreatic cancer tissue compared to the adjacent normal tissue. Consistently, HtrA1 levels were also decreased in two human pancreatic cancer cell lines, PANC-1 and BXPC-3. Moreover, enforced expression of HtrA1 inhibited cell viability and colony formation of PANC-1 and BXPC-3 cells. Overexpression of HtrA1 promoted apoptosis and suppressed migratory ability of tumor cells. On the contrary, siRNA-mediated knockdown of HtrA1 promoted the growth potential of pan...
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research
Glycoprotein nonmetastatic melanoma protein B accelerates tumorigenesis of cervical cancer in vitro by regulating the Wnt/ β-catenin pathway.
In conclusion, we demonstrated that GPNMB contributed to the tumorigenesis of cervical cancer, at least in part, by regulating MMP-2/MMP-9 activity in tumor cells via activation of canonical Wnt/β-catenin signaling. This might be a potential therapeutic target for treating human cervical cancer. PMID: 30484490 [PubMed - in process]
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research
miR-130b participates in wear particle-induced inflammation and osteolysis via FOXF2/NF- κB pathway.
CONCLUSION: The role of miR-130b/FOXF2/NF-κB pathway in PIO was firstly revealed, which provided new targets for the treatment of periprosthetic osteolysis. PMID: 30488739 [PubMed - as supplied by publisher]
Source: Immunopharmacology and Immunotoxicology - November 30, 2018 Category: Allergy & Immunology Tags: Immunopharmacol Immunotoxicol Source Type: research
B-Cell Receptor-Associated Protein 31 Regulates the Expression of Valosin-Containing Protein Through Elf2
Conclusion: These results indicate that Bap31 regulates the expression of VCP possibly via Elf2 and support the potential molecular function of Bap31 in CNS diseases.Cell Physiol Biochem 2018;51:1799 –1814
Source: Cellular Physiology and Biochemistry - November 30, 2018 Category: Cytology Source Type: research
Alpha-naphthoflavone induces apoptosis through endoplasmic reticulum stress via c-Src-, ROS-, MAPKs-, and arylhydrocarbon receptor-dependent pathways in HT22 hippocampal neuronal cells.
Abstract α-Naphthoflavone (αNF) is a prototype flavone, also known as a modulator of aryl hydrocarbon receptor (AhR). In the present study, we investigated the molecular mechanisms of αNF-induced cytotoxic effects in HT22 mouse hippocampal neuronal cells. αNF induced apoptotic cell death via activation of caspase-12 and -3 and increased expression of endoplasmic reticulum (ER) stress-associated proteins, including C/EBP homologous protein (CHOP). Inhibition of ER stress by treatment with the ER stress inhibitor, salubrinal, or by CHOP siRNA transfection reduced αNF-induced cell death. αNF activated mitogen-a...
Source: Neurotoxicology - November 30, 2018 Category: Neurology Authors: Yu AR, Ju Jeong Y, Hwang CY, Yoon KS, Choe W, Ha J, Kim SS, Kim Pak Y, Yeo EJ, Kang I Tags: Neurotoxicology Source Type: research
Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury
AbstractAstrocytes are involved in neuroprotection, and DJ-1 is an important antioxidant protein that is abundantly expressed in reactive astrocytes. However, the role of DJ-1 in astrocytes ’ neuroprotection in cerebral ischemia/reperfusion injury and its potential mechanism is unclear. Thus, to explore effects and mechanisms of DJ-1 on the neuroprotection of astrocytes, we used primary co-cultures of neurons and astrocytes under oxygen and glucose deprivation/reoxygenation in vitro and transient middle cerebral artery occlusion/reperfusion in vivo to mimic ischemic reperfusion insult. Lentiviral was used to inhibit and ...
Source: Journal of Molecular Medicine - November 30, 2018 Category: Molecular Biology Source Type: research
Baicalin inhibits proliferation and promotes apoptosis of vascular smooth muscle cells by regulating the MEG3/p53 pathway following treatment with ox‑LDL.
Baicalin inhibits proliferation and promotes apoptosis of vascular smooth muscle cells by regulating the MEG3/p53 pathway following treatment with ox‑LDL. Int J Mol Med. 2018 Nov 30;: Authors: Liu Y, Jia L, Min D, Xu Y, Zhu J, Sun Z Abstract Atherosclerosis (AS) is a systemic disease associated with lipid metabolic disorders and abnormal proliferation of smooth muscle cells. Baicalin is a flavonoid compound isolated from the dry roots of Scutellaria baicalensis Georgi and exerts anti‑proliferative effects in various types of cells. However, the effect of baicalin on AS remains unclear. In the pre...
Source: International Journal of Molecular Medicine - November 30, 2018 Category: Molecular Biology Authors: Liu Y, Jia L, Min D, Xu Y, Zhu J, Sun Z Tags: Int J Mol Med Source Type: research
ADAM17 knockdown increases sensitivity of SW480 cells to cetuximad.
CONCLUSIONS: ADAM17 knockdown obviously inhibits EGFR-AKT signaling pathway and increases the sensitivity of SW480 cells to tocetuximad. PMID: 30514687 [PubMed - in process]
Source: Journal of Southern Medical University - November 30, 2018 Category: Universities & Medical Training Authors: Chen Y, Zheng K, Chen Z, Feng H, Fang W, Huang Z Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research
SIRT1 participates in epithelial-mesenchymal transition of EC-9706 and Eca-109 cells in vitro by regulating Snail expression.
CONCLUSIONS: SIRT1 participates in the invasion and metastasis of EC-9706 and Eca- 109 cells probably by inducing EMT via regulating the expression of Snail. PMID: 30514680 [PubMed - in process]
Source: Journal of Southern Medical University - November 30, 2018 Category: Universities & Medical Training Authors: Wu Y, Xin D, Liu C, Wang F Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research
Expression of secreted frizzled-related protein 4 in DNA mismatch repair-deficient and mismatch repair-proficient colorectal cancers.
CONCLUSIONS: Up-regulation of SFRP4 in dMMR stage Ⅱ colon cancer promotes apoptosis and inhibits proliferation of the cancer cells, and may improve the prognosis of dMMR colon cancer. PMID: 30514676 [PubMed - in process]
Source: Journal of Southern Medical University - November 30, 2018 Category: Universities & Medical Training Authors: Chen K, Liang H, Peng J, Zheng Y Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research
Effects of sera of rats fed with Huganqingzhi tablets on endoplasmic reticulum stress in a HepG2 cell model of nonalcoholic fatty liver disease.
CONCLUSIONS: HGT serum can effectively prevent FFAs-induced steatosis in HepG2 cells by alleviating ER stress, in which PKC-δ may act as an important target. PMID: 30514673 [PubMed - in process]
Source: Journal of Southern Medical University - November 30, 2018 Category: Universities & Medical Training Authors: Yang M, Chen Z, Xiao C, Tang W, Zhou B Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research
Insulin-like growth factor-1 activates AMPK to augment mitochondrial function and correct neuronal metabolism in sensory neurons in type 1 diabetes
ConclusionsIn DRG neuron cultures IGF-1 signals via AMPK to elevate mitochondrial function and drive axonal outgrowth. We propose that this signaling axis mediates IGF-1-dependent protection from distal dying-back of fibers in diabetic neuropathy.
Source: Molecular Metabolism - November 29, 2018 Category: Endocrinology Source Type: research
Salvianolic acids improve liver lipid metabolism in ovariectomized rats via blocking STAT-3/SREBP1 signaling
In conclusion, these findings illustrated that salvianolic acids markedly alleviated the lipid metabolism disorders and protected against the postmenopausal obesity. The underlying mechanism was probably associated with the regulation of STAT-3 signaling.
Source: Chinese Journal of Natural Medicines - November 29, 2018 Category: Complementary Medicine Source Type: research
DNA replication licensing proteins: saints and sinners in cancer
Publication date: Available online 28 November 2018Source: Seminars in Cancer BiologyAuthor(s): Muhammad Jameel Mughal, Ravikiran Mahadevappa, Hang Fai KwokAbstractDNA replication is all-or-none process in the cell, meaning, once the DNA replication begins it proceeds to completion. Hence, to achieve maximum control of DNA replication, eukaryotic cells employ a multi-subunit initiator protein complex known as “pre-replication complex or DNA replication licensing complex (DNA replication LC). This complex involves multiple proteins which are origin-recognition complex family proteins, cell division cycle–6, chromatin li...
Source: Seminars in Cancer Biology - November 29, 2018 Category: Cancer & Oncology Source Type: research
Ectodomain Shedding of RAGE and TLR4 as a Negative Feedback Regulation in High-Mobility Group Box 1-Activated Aortic Endothelial Cells
Conclusion: In response to HMGB1 stimulation, HAECs rapidly undergo ectodomain shedding of RAGE and TLR4, and thereby become insensitive to further HMGB1 stimulation. ADAM17, activated through RAGE-p38 MAPK pathway, is implicated in the ectodomain cleavage of the receptors.Cell Physiol Biochem 2018;51:1632 –1644
Source: Cellular Physiology and Biochemistry - November 29, 2018 Category: Cytology Source Type: research
Naa15 knockdown enhances c2c12 myoblast fusion and induces defects in zebrafish myotome morphogenesis
Publication date: Available online 29 November 2018Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Olivier Monestier, Aurélie Landemaine, Jérôme Bugeon, Pierre-Yves Rescan, Jean-Charles GabillardAbstractThe understanding of muscle tissue formation and regeneration is essential for the development of therapeutic approaches to treat muscle diseases or loss of muscle mass and strength during ageing or cancer. One of the critical steps in muscle formation is the fusion of muscle cells to form or regenerate muscle fibres.To identify new genes controlling myoblast fusion, w...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 29, 2018 Category: Molecular Biology Source Type: research
Long Noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410.
This study was undertaken to investigate the underlying mechanisms of long noncoding RNA OIP5-AS1 via regulating miR-410 to modulate Wnt-7b in the progression of glioma. To address this problem, we measured the expression of OIP5-AS1 and miR-410 in glioma tissues by qRT-PCR. Glioma U87 cells were transfected with OIP5-AS1 siRNA or miR-410 inhibitors. The targeting relationships among miR-410, OIP5-AS1 and Wnt-7b were verified by luciferase reporter assays. Western blotting was employed to determine the expression of Wnt-7b/β-catenin pathway-related proteins, while MTT, flow cytometry, Transwell assays, and wound-healing a...
Source: Bioscience Reports - November 29, 2018 Category: Biomedical Science Authors: Sun WL, Kang T, Wang YY, Sun JP, Li C, Liu HJ, Yang Y, Jiao BH Tags: Biosci Rep Source Type: research
Resveratrol treatment reduces the vulnerability of SH-SY5Y cells and cortical neurons overexpressing SOD1-G93A to Thimerosal toxicity through SIRT1/DREAM/PDYN pathway.
Abstract In humans, mutation of glycine 93 to alanine of Cu++/Zn++ superoxide dismutase type-1 (SOD1-G93 A) has been associated to some familial cases of Amyotrophic Lateral Sclerosis (ALS). Several evidence proposed the involvement of environmental pollutants that like mercury could accelerate ALS symptoms. SH-SY5Y cells stably transfected with SOD1 and G93 A mutant of SOD1 constructs were exposed to non-toxic concentrations (0.01 µM) of ethylmercury thiosalicylate (thimerosal) for 24 hours. Interestingly, we found that thimerosal, in SOD1-G93 A cells, but not in SOD1 cells, reduced cell survival. Furthe...
Source: Neurotoxicology - November 29, 2018 Category: Neurology Authors: Laudati G, Mascolo L, Guida N, Sirabella R, Pizzorusso V, Bruzzaniti S, Serani A, Di Renzo G, Canzoniero LM, Formisano L Tags: Neurotoxicology Source Type: research
Overcoming Resistance to AC0010, a Third Generation of EGFR Inhibitor, by Targeting c-MET andBCL-2.
Abstract AC0010 is a pyrrolopyrimidine-based irreversible inhibitor of epidermal growth factor receptor (EGFR), structurally distinct from previously reported pyrimidine-based irreversible EGFR inhibitors such as osimertinib and rociletinib. AC0010 selectively inhibits EGFR T790M mutation in both preclinical and clinical studies. However, AC0010 treatment eventually triggers drug resistance with unknown mechanism. To this end, we established two H1975 NSCLC-derived lines resistant to AC0010 after a series of drug exposure and selection in either nude-mice xenograft tumor (H1975-P) or cell culture (H1975-AVR) setti...
Source: Neoplasia - November 29, 2018 Category: Cancer & Oncology Authors: Xu W, Tang W, Li T, Zhang X, Sun Y Tags: Neoplasia Source Type: research
Serum Extracellular Vesicles Retard H9C2 Cell Senescence by Suppressing miR-34a Expression
In conclusion, our study suggests that serum EVs retard H9C2 cell senescence through the miR-34a/PNUTS pathway, providing a potential therapy for cardiac aging.
Source: Journal of Cardiovascular Translational Research - November 29, 2018 Category: Cardiology Source Type: research
Naa15 knockdown enhances c2c12 myoblast fusion and induces defects in zebrafish myotome morphogenesis.
Abstract The understanding of muscle tissue formation and regeneration is essential for the development of therapeutic approaches to treat muscle diseases or loss of muscle mass and strength during ageing or cancer. One of the critical steps in muscle formation is the fusion of muscle cells to form or regenerate muscle fibres. To identify new genes controlling myoblast fusion, we performed a siRNA screen in c2c12 myoblasts. The genes identified during this screen were then studied in vivo by knockdown in zebrafish using morpholino. We found that N-alpha-acetyltransferase 15 (Naa15) knockdown enhanced c2c12 myoblas...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - November 29, 2018 Category: Biochemistry Authors: Monestier O, Landemaine A, Bugeon J, Rescan PY, Gabillard JC Tags: Comp Biochem Physiol B Biochem Mol Biol Source Type: research
Palmatine attenuated dextran sulfate sodium (DSS)-induced colitis via promoting mitophagy-mediated NLRP3 inflammasome inactivation
Publication date: January 2019Source: Molecular Immunology, Volume 105Author(s): Chu-Tian Mai, Mei-Mei Wu, Chun-Li Wang, Zi-Ren Su, Yuan-Yuan Cheng, Xiao-Jun ZhangAbstractActivation of NLRP3 inflammasomes is crucial in the pathological process of Ulcerative colitis (UC), which could be negatively regulated by PINK1/Parkin-driven mitophagy. Palmatine is a herb derived isoquinoline alkaloid with potent anti-inflammatory and anti-bacteria activities. In present study, we evaluated the effect of palmatine on dextran sulfate sodium (DSS)-induced mice colitis and examined whether its effect is exerted by promoting mitophagy-medi...
Source: Molecular Immunology - November 28, 2018 Category: Allergy & Immunology Source Type: research
microRNA-29a-3p, Up-Regulated in Human Gastric Cells and Tissues with < b > < i > H.Pylori < /i > < /b > Infection, Promotes the Migration of GES-1 Cells via A20-Mediated EMT Pathway
Conclusion: The miR-29a-3p may act as a tumor promotive miRNA by regulating cells migration through directly targeting of A20 gene in human gastric epithelial cells infected withH.pylori.Cell Physiol Biochem 2018;51:1250 –1263
Source: Cellular Physiology and Biochemistry - November 28, 2018 Category: Cytology Source Type: research
P27 Protects Cardiomyocytes from Sepsis via Activation of Autophagy and Inhibition of Apoptosis.
CONCLUSIONS The study data demonstrated that the overexpression of p27 protects cardiomyocytes from sepsis-induced cardiac depression via the activation of autophagy and inhibition of apoptosis. PMID: 30478251 [PubMed - in process]
Source: Medical Science Monitor - November 28, 2018 Category: Research Tags: Med Sci Monit Source Type: research
Apoptosis of A549 cells by small interfering RNA targeting survivin delivery using poly-β-amino ester/guanidinylated O-carboxymethyl chitosan nanoparticles
This study focuses on guanidinylated O-carboxymethyl chitosan (GOCMCS) along with poly-β-amino ester(PBAE) for siRNA delivery. Binding efficiency of PBAE/siRNA/GOCMCS nanoparticles were characterized by gel electrophoresis. The siRNA-loaded nanoparticles were found to be stable in the presence of RNase A, serum and BALF respectively. Fine particle fraction (FPF) which was determined by a two-stage impinger (TSI) was 57.8% ± 2.6%. The particle size and zeta potential of the nanoparticles were 153.8 ± 12.54 nm and +12.2 ± 4.94 mV. In vitro cell transfection studies were carried out with A549 cells. The cellular uptake wa...
Source: Asian Journal of Pharmaceutical Sciences - November 28, 2018 Category: Drugs & Pharmacology Source Type: research
STIM promotes the epithelial-mesenchymal transition of podocytes through regulation of Fc γRII activity in diabetic nephropathy.
CONCLUSION: Our study suggests that STIM and FcγRII play an essential role in the regulation of DKD-induced podocyte EMT. STIM is an essential component of FcγR activation and inhibition of STIM-mediated signaling pathway might be a new strategy to treat IgG-dependent renal diseases. PMID: 30475382 [PubMed - as supplied by publisher]
Source: Histology and Histopathology - November 28, 2018 Category: Cytology Tags: Histol Histopathol Source Type: research
Targeted regulation by ROCK2 on bladder carcinoma via Wnt signaling under hypoxia.
Authors: Luo J, Lou Z, Zheng J Abstract Bladder cancer is frequently occurred in urinary system and has complicated pathogenesis factors including both genetics and environmental factors that have not been fully illustrated. Hypoxia can further induce tumor progression. ROCK2 has abnormal expression in various tumors but its expression or functional role in bladder cancer have not been illustrated. In vitro cultured bladder cancer cell line T24 was randomly assigned into control group, hypoxia group (prepared under hypoxic culture), and ROCK2 siRNA group (transfected with ROCK2 siRNA after hypoxia treatment). Real-...
Source: Cancer Biomarkers - November 28, 2018 Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research
MicroRNA-21 silencing prolongs islet allograft survival by inhibiting Th17 cells.
In this study, we examined the impact of systemic administration of miR-21 inhibitor on allografts in a pancreatic islet transplantation model. We showed that specific miR-21 silencing in vivo significantly prolonged allograft survival (median survival time (MST) 21 days with antagomiR-21 vs 13 days for the control, p < 0.05), and the change was associated with a decrease in Th17 cells (~3-fold) and an increase in B10 cells (~2.4-fold). Moreover, we found that miR-21-silenced B cells mediate this protective role through pro-inflammatory Th17 responses in an IL-10-dependent fashion. Thus, we have revealed a novel...
Source: International Immunopharmacology - November 28, 2018 Category: Allergy & Immunology Authors: Wang H, Fan H, Tao J, Shao Q, Ding Q Tags: Int Immunopharmacol Source Type: research
Exosomal KLF3-AS1 from hMSCs promoted cartilage repair and chondrocyte proliferation in osteoarthritis
The present study was designed to explore whether exosomal lncRNA-KLF3-AS1 derived from human mesenchymal stem cells (hMSCs) can serve as a positive treatment for osteoarthritis (OA). hMSCs and MSC-derived exosomes (MSC-exo) were prepared for morphological observation and identification by transmission electron microscopy and flow cytometry. IL-1β-induced OA chondrocytes and collagenase-induced rat model of OA were established for the further experiments. Lentivirus-mediated siRNA targeting KLF3-AS1 was transfected into MSCs for silencing KLF3-AS1. The real-time quantitative PCR and western blotting analysis were perf...
Source: Biochemical Journal - November 28, 2018 Category: Biochemistry Authors: Liu, Y., Zou, R., Wang, Z., Wen, C., Zhang, F., Lin, F. Tags: Research Articles Source Type: research
Malat1 long noncoding RNA regulates inflammation and leukocyte differentiation in experimental autoimmune encephalomyelitis
In this study, we investigated the contributions of the MALAT1 long noncoding RNA to autoimmune neuroinflammation in central nervous system tissues from patients with multiple sclerosis (MS) and mice with experimental autoimmune encephalomyelitis (EAE). Expression of MALAT1 was decreased in the spinal cords of EAE mice as well as in stimulated splenocytes and primary macrophages. MALAT1 downregulation by specific siRNAs enhanced the polarization of macrophages towards the M1 phenotype. Interestingly, siRNA-mediated MALAT1 downregulation shifted the pattern of T-cell differentiation towards a Th1/Th17 cell profile and decre...
Source: Journal of Neuroimmunology - November 28, 2018 Category: Allergy & Immunology Authors: Farimah Masoumi, Samira Ghorbani, Farideh Talebi, William Branton, Samira Rajaei, Christopher Power, Farshid Noorbakhsh Source Type: research
Identification and characterization of GLDC as host susceptibility gene to severe influenza
Glycine decarboxylase (GLDC) modulates host antiviral response upon viral infection due to the existence of GLDC ‐pyrimidine biosynthesis‐innate immunity axis. The inter‐individual differential GLDC expression derived from genetic variation may dictate the susceptibility to severe influenza. AbstractGlycine decarboxylase (GLDC) was prioritized as a candidate susceptibility gene to severe influenza in humans. The higher expression of GLDC derived from genetic variations may confer a higher risk to H7N9 and severe H1N1 infection. We sought to characterize GLDC as functional susceptibility gene that GLDC may intrinsical...
Source: EMBO Molecular Medicine - November 28, 2018 Category: Molecular Biology Authors: Jie Zhou, Dong Wang, Bosco Ho ‐Yin Wong, Cun Li, Vincent Kwok‐Man Poon, Lei Wen, Xiaoyu Zhao, Man Chun Chiu, Xiaojuan Liu, Ziwei Ye, Shuofeng Yuan, Kong‐Hung Sze, Jasper Fuk‐Woo Chan, Hin Chu, Kelvin Kai‐Wang To, Kwok Yung Yuen Tags: Research Article Source Type: research
FAM3B (PANDER) functions as a co-activator of FOXO1 to promote gluconeogenesis in hepatocytes.
In conclusion, PANDER protein is abundantly present in the nucleus, where it functions as a new co-activator of FOXO1 to induce gluconeogenic gene expression in hepatocytes. PMID: 30488666 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - November 28, 2018 Category: Molecular Biology Authors: Chi Y, Meng Y, Wang J, Yang W, Wu Z, Li M, Wang D, Gao F, Geng B, Tie L, Zhang W, Yang J Tags: J Cell Mol Med Source Type: research
Inhibition of non-receptor tyrosine kinase Src induces phosphoserine 256-independent aquaporin-2 membrane accumulation.
In conclusion, Src inhibition causes phosphorylation of S269 independently of pS256, and induces AQP2 membrane accumulation by inhibiting clathrin-mediated endocytosis and increasing exocytosis. We conclude that S269 can be phosphorylated without pS256, and pS269 alone is important for AQP2 apical membrane accumulation under some conditions. These data increase our understanding of the independent pathways that can phosphorylate different residues in the AQP2 c-terminus, and suggest new strategies to target distinct AQP2 serine residues to induce membrane expression of this water channel when VP signaling is defective. Thi...
Source: The Journal of Physiology - November 28, 2018 Category: Physiology Authors: Cheung PW, Terlouw A, Janssen SA, Brown D, Bouley R Tags: J Physiol Source Type: research
Inhibition of TREM1 reduces inflammation and oxidative stress after spinal cord injury (SCI) associated with HO-1 expressions
Publication date: January 2019Source: Biomedicine & Pharmacotherapy, Volume 109Author(s): Zhenhuan Li, Furong Wu, Dafeng Xu, Zhongzheng Zhi, Guanghui XuAbstractSpinal cord injury (SCI) is a devastating event, leading to the progression of chronic neuropathic pain syndrome. Triggering receptor expressed on myeloid cells 1 (TREM1) is an innate immune receptor expressed on neutrophils and monocytes/macrophages. TREM1 enhances inflammatory response in various models of diseases, but its significance in SCI remains unclear. In the present study, we attempted to explore the effects of TREM1 on the regulation of SCI. Spinal cord ...
Source: Biomedicine and Pharmacotherapy - November 27, 2018 Category: Drugs & Pharmacology Source Type: research