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Intraperitoneal Nanotherapy for Metastatic Ovarian Cancer Based on siRNA-Mediated Suppression of DJ-1 Protein Combined with a Low Dose of Cisplatin
Publication date: Available online 7 April 2018 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Canan Schumann, Stephanie Chan, Jess A. Millar, Yuliya Bortnyak, Katherine Carey, Alex Fedchyk, Leon Wong, Tetiana Korzun, Abraham S. Moses, Anna Lorenz, Delany Shea, Olena Taratula, Oleh Khalimonchuk, Oleh Taratula Herein, we report an efficient combinatorial therapy for metastatic ovarian cancer based on siRNA-mediated suppression of DJ-1 protein combined with a low dose of cisplatin. DJ-1 protein modulates, either directly or indirectly, different oncogenic pathways that support and promote survival, gro...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - April 8, 2018 Category: Nanotechnology Source Type: research
Protective effect of Gelofusine against cRGD-siRNA-induced nephrotoxicity in mice.
Authors: Liao W, Qin Y, Liao L, Cen B, Wu Z, Wei Y, Wang Z, Li G, Ji A Abstract Based on successful targeting to the αvβ3 integrin of cyclic arginine-glycine-aspartic acid (cRGD), cRGD-conjugated small interfering RNA (siRNA) exhibits tumor targeting and has become a new treatment strategy for solid tumors. However, the nephrotoxicity caused by its renal retention limits its clinical application. Here, we evaluated the protective effect of Gelofusine against cRGD-conjugated siRNA-induced nephrotoxicity in mice. Male Kunming mice (six per group) were either co-injected with Gelofusine and cRGD-siRNA or injected wi...
Source: Renal Failure - April 7, 2018 Category: Urology & Nephrology Tags: Ren Fail Source Type: research
Rosmarinic acid and siRNA combined therapy represses Hsp27 (HSPB1) expression and induces apoptosis in human glioma cells.
Abstract High expression of Hsp27 in glioma cells has been closely associated with tumor cell proliferation and apoptosis inhibition. The aim of the present study was to asses the effects of rosmarinic acid (RA) on Hsp27 expression and apoptosis in non-transfected and transfected human U-87 MG cells. The effect of rosmarinic acid was compared to quercetin, which is known to be a good Hsp27 inhibitor. In order to block the expression of Hsp27 gene (HSPB1), transfection with specific siRNAs was performed. Western blotting technique was used to assess the Hsp27 expression, and caspase-3 colorimetric activity assay wa...
Source: Cell Stress and Chaperones - April 7, 2018 Category: Cytology Authors: Şengelen A, Önay-Uçar E Tags: Cell Stress Chaperones Source Type: research
Intraperitoneal Nanotherapy for Metastatic Ovarian Cancer Based on siRNA-Mediated Suppression of DJ-1 Protein Combined with a Low Dose of Cisplatin
Herein, we report an efficient combinatorial therapy for metastatic ovarian cancer based on siRNA-mediated suppression of DJ-1 protein combined with a low dose of cisplatin. DJ-1 protein modulates, either directly or indirectly, different oncogenic pathways that support and promote survival, growth, and invasion of ovarian cancer cells. To evaluate the potential of this novel therapy, we have engineered a cancer-targeted nanoplatform and validated that DJ-1 siRNA delivered by this nanoplatform after intraperitoneal injection efficiently downregulates the DJ-1 protein in metastatic ovarian cancer tumors and ascites.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - April 7, 2018 Category: Nanotechnology Authors: Canan Schumann, Stephanie Chan, Jess A. Millar, Yuliya Bortnyak, Katherine Carey, Alex Fedchyk, Leon Wong, Tetiana Korzun, Abraham S. Moses, Anna Lorenz, Delany Shea, Olena Taratula, Oleh Khalimonchuk, Oleh Taratula Source Type: research
Effect of dihydroarteminin combined with siRNA targeting Notch1 on Notch1/c-Myc signaling in T-cell lymphoma cells.
Authors: Huo L, Wei W, Wu S, Zhao X, Zhao C, Zhao H, Sun L Abstract The effectiveness of therapy combining dihydroartemisinin (DHA) and small interfering RNA targeting Notch1 (siNotch1) in T-cell lymphoma remains unknown. The present study explored the potential and possible mechanisms of combined dihydroarteminin, and siNotch1 therapy for T-cell lymphoma. It was demonstrated that the viability rates of siRNA-DHA-treated cells was significantly suppressed in comparison with those in control cells, control siRNA cells, siRNA-treated cells and DHA-treated cells (P<0.01). Additionally, there was a significant incre...
Source: Experimental and Therapeutic Medicine - March 31, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research
GSE108262 Comparison of STEEL siRNA-treated and control siRNA-treated human endothelial cells
Contributors : Philip A Marsden ; H S ManSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo determine the role of STEEL in endothelial cell (EC) gene regulation, gene expression analysis was conducted on control and STEEL siRNA-treated human dermal microvascular endothelial cells (HMVECs) and human umbilical vein endothelial cells (HUVECs). A total of 225 protein-coding genes were downregulated and 80 were upregulated when both EC types were grouped for analysis. In HMVEC alone, 544 protein-coding genes were downregulated and 218 were upregulated. In HUVEC alone, 177 protein-coding genes were downregulate...
Source: GEO: Gene Expression Omnibus - March 30, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
Complexation of Chol-DsiRNA in place of Chol-siRNA greatly increases the duration of mRNA suppression by polyplexes of PLL(30)-PEG(5K) in primary murine syngeneic breast tumors after i.v. administration
Publication date: 30 May 2018 Source:International Journal of Pharmaceutics, Volume 543, Issues 1–2 Author(s): Vishakha V. Ambardekar, Rajesh R. Wakaskar, Zhen Ye, Stephen M. Curran, Timothy R. McGuire, Don W. Coulter, Rakesh K. Singh, Joseph A. Vetro RNA interference has tremendous potential for cancer therapy but is limited by the insufficient potency of RNAi molecules after i.v. administration. We previously found that complexation with PLL(30)-PEG(5K) greatly increases the potency of 3′-cholesterol-modified siRNA [Chol-siRNA] in primary murine syngeneic 4T1 breast tumors after i.v. administration but mRNA suppress...
Source: International Journal of Pharmaceutics - March 30, 2018 Category: Drugs & Pharmacology Source Type: research
Vitamin A-coupled liposomes containing siRNA against HSP47 ameliorate skin fibrosis in chronic graft-versus-host disease
Chronic graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (SCT) is characterized by multiorgan fibrosis and profoundly affects the quality of life of transplant survivors. Heat shock protein 47 (HSP47), a collagen-specific molecular chaperone, plays a critical role in collagen synthesis in myofibroblasts. We explored the role of HSP47 in the fibrotic process of cutaneous chronic GVHD in mice. Immunohistochemical analysis showed massive fibrosis with elevated amounts of collagen deposits and accumulation of F4/80+ macrophages, as well as myofibroblasts expressing HSP47 and retinol-bin...
Source: Blood - March 29, 2018 Category: Hematology Authors: Yamakawa, T., Ohigashi, H., Hashimoto, D., Hayase, E., Takahashi, S., Miyazaki, M., Minomi, K., Onozawa, M., Niitsu, Y., Teshima, T. Tags: Transplantation Source Type: research
Tivanisiran, a novel siRNA for the treatment of dry eye disease.
Authors: Moreno-Montañés J, Bleau AM, Jimenez AI Abstract INTRODUCTION: Dry eye disease (DED) is characterized by an alteration of the tear film with ocular inflammation and neurosensory abnormalities. The main clinical signs of this condition are tear instability and ocular damage. Although DED has gained significant attention in the past few years, limited prescription treatment options are available for patients. Areas covered: The current manuscript summarizes the pre-clinical and clinical development of tivanisiran, a novel small interfering oligonucleotide of RNA (siRNA) used for the treatment of DED. Tivan...
Source: Expert Opinion on Investigational Drugs - March 28, 2018 Category: Drugs & Pharmacology Tags: Expert Opin Investig Drugs Source Type: research
i-Fect Delivers Again!
Knocks Down Suspected Stress/Anxiety ReceptorThe molecular pathogenesis underlying anxiety disorders is still unclear. Here, the authors demonstrate that myristoylated alanine-rich C-kinase substrate like 1 (MARCKSL1) overexpression in mice increases spine formation in the amygdala and induces stress hormone upregulation and anxiety-like behaviors. Suppression of MARCKSL1 in the amygdala ameliorates both the increase in stress hormones and the elevated anxiety-like behaviors. Our results indicate that MARCKSL1 expression in the amygdala plays an important role in anxiety-like behaviors.This was proved, in part, by the knoc...
Source: siRNA and DsiRNA Transfection Efficiency - March 28, 2018 Category: Neuroscience Tags: Amygdala Anxiety i-Fect MARCKSL1 Stress Source Type: news
Inhibition of BMP4 and Alpha Smooth Muscle Actin Expression in LX-2 Hepatic Stellate Cells by BMP4-siRNA Lipid Based Nanoparticle.
Conclusion: VA-coated liposomes were successfully designed to deliver BMP4-siRNA to specifically target HSC. The novel delivery system discussed herein may serve as a potential therapeutic strategy for the treatment of liver fibrosis in the future. This article is open to POST-PUBLICATION REVIEW. Registered readers (see "For Readers") may comment by clicking on ABSTRACT on the issue's contents page. PMID: 29578859 [PubMed - in process]
Source: J Pharm Pharm Sci - March 28, 2018 Category: Drugs & Pharmacology Authors: Omar R, Yang J, Alrushaid S, Burczynski FJ, Minuk GY, Gong Y Tags: J Pharm Pharm Sci Source Type: research
Improving Bone Regeneration Using Chordin siRNA Delivered by pH-Responsive and Non-Toxic Polyspermine Imidazole-4,5-Imine
Conclusions: Our results suggest that Chordin is a potential target for improving osteogenesis and bone nonunion therapy and that responsive and non-toxic cationic polyimines such as PSI are therapeutically feasible carriers for the packaging and delivery of Chordin siRNA to hBMSCs.Cell Physiol Biochem 2018;46:133 –147
Source: Cellular Physiology and Biochemistry - March 26, 2018 Category: Cytology Source Type: research
DNA nanoclew templated spherical nucleic acids for siRNA delivery
Chem. Commun., 2018, Advance Article DOI: 10.1039/C7CC09257A, CommunicationWeimin Ruan, Meng Zheng, Yang An, Yuanyuan Liu, David B. Lovejoy, Mingcong Hao, Yan Zou, Albert Lee, Shu Yang, Yiqing Lu, Marco Morsch, Roger Chung, Bingyang Shi A superior biocompatible spherical nucleic acid (SNA) conjugate was fabricated by grafting siRNA onto the surface of a core composed of a spherical DNA nanostructure that we have termed DNA nanoclew (DC). To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 23, 2018 Category: Chemistry Authors: Weimin Ruan Source Type: research
GSE112007 Next Generation Sequencing Facilitates Quantitative of Transcriptomes in Various Cells Treated with or without LD100 and siRNA of SOD1
Conclusions: The global transcriptomic data on HeLa, DU145 and RWPE-1 after SOD1 inhibition using its specific inhibitor-LD100 indicated that the most changed expression upon this interruption of ROS homeostasis is the key genes in plenty of signaling pathways inside cancer cells, not inside normal cells, although mRNA levels of numerous genes are altered in three lines of cells. The modulation of gene expression leads to repression of multiple signaling pathways including ERK, PI3K and NF-KB and their crosstalk to promote cell growth and activation of the signaling pathways to cause both cell cycle arrest and apoptosis. T...
Source: GEO: Gene Expression Omnibus - March 23, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Transdermal anti-nuclear kappaB siRNA therapy for atopic dermatitis using a combination of two kinds of functional oligopeptide
Publication date: 5 May 2018 Source:International Journal of Pharmaceutics, Volume 542, Issues 1–2 Author(s): Hisako Ibaraki, Takanori Kanazawa, Yuuki Takashima, Hiroaki Okada, Yasuo Seta Nucleic acid-based targeting of nuclear factor kappaB (NF-κB) is gaining attention as a treatment option for skin diseases like atopic dermatitis (AD). Transdermal administration improves patient quality of life because of non-invasive; however, siRNA delivery into the skin can be challenging owing to the barrier of tight junctions in the granular layer. Therefore, we aimed to develop a delivery system of siRNA for topical skin applic...
Source: International Journal of Pharmaceutics - March 23, 2018 Category: Drugs & Pharmacology Source Type: research
In Vitro TyRP-1 Knockdown Based on siRNA Carried by Liquid Crystalline Nanodispersions: an Alternative Approach for Topical Treatment of Vitiligo
ConclusionsWe demonstrated that the liquid crystalline nanodispersions showed promising alternative for the topical treatment of vitiligo due to their physical parameters and ability in knockdown the target protein involved with autoimmune destruction of melanocytes.
Source: Pharmaceutical Research - March 20, 2018 Category: Drugs & Pharmacology Source Type: research
Structurally modulated codelivery of siRNA and Argonaute 2 for enhanced RNA interference Engineering
Small interfering RNA (siRNA) represents a promising class of inhibitors in both fundamental research and the clinic. Numerous delivery vehicles have been developed to facilitate siRNA delivery. Nevertheless, achieving highly potent RNA interference (RNAi) toward clinical translation requires efficient formation of RNA-induced gene-silencing complex (RISC) in the cytoplasm. Here we...
Source: Proceedings of the National Academy of Sciences - March 20, 2018 Category: Science Authors: Jiahe Li, Connie Wu, Wade Wang, Yanpu He, Elad Elkayam, Leemor Joshua-Tor, Paula T. Hammond Tags: PNAS Plus Source Type: research
GSE83634 Inhibition of NF- κB signaling by a novel siRNA sequence induces apoptosis and suppresses tumor growth in KRAS mutant colorectal cancer.
In this study, we aimed to discover an effective therapeutic microRNA (miRNA) that could target KRAS mutant CRC. For this purpose, we investigated the functional relevance of dysregulated miRNAs in KRAS mutant cancers. We transfected exogenous KRASG12V into human embryonic kidney 293 (HEK293) cells and human lung fibroblasts (MRC5) cells, and performed comprehensive microRNA expression profiling by microarray analysis. The results showed that 6 miRNAs are significantly upregulated in KRAS-transfected HEK293 and MRC5 cells. Among 6 miRNAs, we identified mature miRNA-29b-1-5p as potent growth inhibitor in CRC cell proliferat...
Source: GEO: Gene Expression Omnibus - March 17, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
Antitumor activity of kinetochore-associated protein 2 siRNA against lung cancer patient-derived tumor xenografts.
Authors: Makita Y, Teratani M, Murata S, Hoashi Y, Matsumoto S, Kawamata Y Abstract It has been widely reported that patient-derived tumor xenografts (PDXs) are more similar to tumor tissues than conventional cancer cell lines. Kinetochore-associated protein 2 (KNTC2) is known to be upregulated specifically in tumor tissues of cancer patients and is recognized as a potential target for cancer therapy. Previously, in vivo antitumor activities of KNTC2 short interfering RNA encapsulated into a lipid nanoparticle (KNTC2-LNP) were reported in orthotopic hepatocellular carcinoma mouse models. However, it remains unclear...
Source: Oncology Letters - March 16, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
Structural basis of siRNA recognition by TRBP double-stranded RNA binding domains
The accurate cleavage of pre-micro(mi)RNAs by Dicer and mi/siRNA guide strand selection are important steps in forming the RNA-induced silencing complex (RISC). The role of Dicer binding partner TRBP in these processes remains poorly understood. Here, we solved the solution structure of the two N-terminal dsRNA binding domains (dsRBDs) of TRBP in complex with a functionally asymmetric siRNA using NMR, EPR, and single-molecule spectroscopy. We find that siRNA recognition by the dsRBDs is not sequence-specific but rather depends on the RNA shape. The two dsRBDs can swap their binding sites, giving rise to two equally populat...
Source: EMBO Journal - March 15, 2018 Category: Molecular Biology Authors: Masliah, G., Maris, C., König, S. L., Yulikov, M., Aeschimann, F., Malinowska, A. L., Mabille, J., Weiler, J., Holla, A., Hunziker, J., Meisner-Kober, N., Schuler, B., Jeschke, G., Allain, F. H.-T. Tags: RNA Biology, Structural Biology Articles Source Type: research
DNA nanoclew templated spherical nucleic acid for siRNA delivery
Chem. Commun., 2018, Accepted Manuscript DOI: 10.1039/C7CC09257A, CommunicationWeimin Ruan, Meng Zheng, Yang An, Yuanyuan Liu, David Lovejoy, Mingcong Hao, Yan Zou, Albert Lee, Shu Yang, Yiqing Lu, Marco Morsch, Roger Chung, Bingyang Shi A superior biocompatible spherical nucleic acid (SNA) conjugate was fabricated by grafting siRNA onto the surface of a core composed of a spherical DNA nanostucture we have termed a DNA... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 14, 2018 Category: Chemistry Authors: Weimin Ruan Source Type: research
Near-infrared light-activatable siRNA delivery by microcapsules for combined tumour therapy.
Authors: Rui Y, Pang B, Zhang J, Liu Y, Hu H, Liu Z, Ama Baidoo S, Liu C, Zhao Y, Li S Abstract A polyelectrolyte microcapsule-based layer-by-layer (LbL) technique has been widely used as a multifunctional vehicle for combined tumor therapy. Meanwhile, with the rapid development of combined tumour therapy, the challenge for designing multifunctional drug delivery system has attracted much more attention. Herein, we developed a new type of microcapsule (MC) system called MPA@siRNA@DOX@MC, which conjugated with siRNA and DOX as well as ICG-Der-02 (MPA) by electrostatic absorption. MPA as indocyanine green (ICG) fluor...
Source: Artificial Cells, Nanomedicine and Biotechnology - March 14, 2018 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research
"siRNA traffic lights": Arabino-configured 2'-anchors for fluorescent dyes are key for dual color readout in cell imaging
Org. Biomol. Chem., 2018, Accepted Manuscript DOI: 10.1039/C8OB00417J, PaperJeannine Steinmeyer Steinmeyer, Heidi-Kristin Walter, Mathilde Bichelberger, Violetta Schneider, Tomas Kubar, Franziska Ronicke, Bettina Olshausen, Karin Nienhaus, Gerd Ulrich Nienhaus, Ute Schepers, Marcus Elstner, Hans-Achim Wagenknecht Two fluorescent dyes are covalently attached in diagonal interstrand orientation to siRNA undergo an energy transfer and thereby enable a dual color fluorescent readout (red/green) for hybridization. Three different structural... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic and Biomolecular Chemistry - March 13, 2018 Category: Molecular Biology Authors: Jeannine Steinmeyer Steinmeyer Source Type: research
A black phosphorus nanosheet-based siRNA delivery system for synergistic photothermal and gene therapy
Chem. Commun., 2018, Advance Article DOI: 10.1039/C8CC00931G, CommunicationHuimeng Wang, Lin Zhong, Yang Liu, Xin Xu, Chao Xing, Min Wang, Shu-Meng Bai, Chun-Hua Lu, Huang-Hao Yang A new siRNA delivery platform based on black phosphorus nanosheets is constructed which shows excellent therapy efficiency for tumors. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 12, 2018 Category: Chemistry Authors: Huimeng Wang Source Type: research
A black phosphorus nanosheet-based siRNA delivery system for synergistic photothermal and gene therapy.
Abstract Gene therapy with small interfering RNA (siRNA) has been proved to be a promising technology to treat various diseases by hampering the production of target proteins. However, developing a delivery system that has high efficiency in transporting siRNA without obvious side effects remains a challenge. Herein, we designed a new survivin siRNA delivery system based on polyethyleneimine functionalized black phosphorus (BP) nanosheets which could suppress tumor growth by silencing survivin expression. Combined with the photothermal properties of the BP nanosheets, the presented delivery system shows excellent ...
Source: Chemical Communications - March 12, 2018 Category: Chemistry Authors: Wang H, Zhong L, Liu Y, Xu X, Xing C, Wang M, Bai SM, Lu CH, Yang HH Tags: Chem Commun (Camb) Source Type: research
Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing
In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab′ to siRNA/SMLs enhanced EGFR-specifi...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - March 6, 2018 Category: Biochemistry Source Type: research
101PDelivery of E-cadherin (CDH1) gene and epidermal growth factor receptor (EGFR) siRNA, using carbonate apatite as a promising vehicle in treatment of breast cancer
Background: E-cadherin (CDH1), a cell adhesion glycoprotein is widely associated with breast cancer where it is involved in tumor growth, invasion and metastasis. Down regulation of its gene is hall mark for epithelial-mesenchymal transition, which is an essential process for tumor metastasis. While various pathways are associated with E-cadherin, one of the prominent signaling pathways is via epidermal growth factor reception (EGFR), which is found to be over expressed. Silencing the over expressed EGFR gene by using a specific short-interfering RNA (siRNA), or inducing the expression of CDH1 gene with the help of a plasm...
Source: Annals of Oncology - March 5, 2018 Category: Cancer & Oncology Source Type: research
Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA.
Authors: Capel V, Vllasaliu D, Watts P, Clarke PA, Luxton D, Grabowska AM, Mantovani G, Stolnik S Abstract Despite research efforts full potential of siRNA-based therapeutics has not yet been fully realized due to a need for suitable, effective delivery formulations. Here, we examine a potential of a new class of water-soluble chitosans as siRNA platform for pulmonary delivery. The system is based on piperazine-substituted chitosans, a material designed to integrate established, safe application of chitosan for mucosal administration with novel properties: the piperazine-substituted chitosans are freely water-solub...
Source: Drug Delivery - March 3, 2018 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research
Black Phosphorus Nanosheets-Based siRNA Delivery System for Synergistic Photothermal and Gene Therapy
Chem. Commun., 2018, Accepted Manuscript DOI: 10.1039/C8CC00931G, CommunicationHuimeng Wang, Lin Zhong, Yang Liu, Xin Xu, Chao Xing, Min Wang, Shumeng Bai, Chun-Hua Lu, Huanghao Yang Gene therapy with small interfering RNA (siRNA) has been proved to be the promising technology to treat various diseases by hampering the production of target proteins. However, developing a delivery... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 2, 2018 Category: Chemistry Authors: Huimeng Wang Source Type: research
siRNA-mediated inhibition of skNAC and Smyd1 expression disrupts myofibril organization: Immunofluorescence and electron microscopy study in C2C12 cells
Publication date: May 2018 Source:Micron, Volume 108 Author(s): Janine Berkholz, Regina Eberle, Klaus Boller, Barbara Munz skNAC (skeletal and heart muscle-specific variant of nascent polypeptide-associated complex) and Smyd1 (SET and MYND domain-containing 1) form a protein dimer which is specific for striated muscle cells. Its function is largely unknown. On the one hand, skNAC-Smyd1 appears to control transcriptional processes in the nucleus, on the other hand, specifically at later stages of myogenic differentiation, both proteins translocate to the sarcoplasm and at least Smyd1 specifically associates with sarcomeric...
Source: Micron - March 1, 2018 Category: Biology Source Type: research
Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing.
In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab' to siRNA/SMLs enhanced EGFR-specific ...
Source: Biochimica et Biophysica Acta - February 28, 2018 Category: Biochemistry Authors: Mokhtarieh AA, Lee J, Kim S, Lee MK Tags: Biochim Biophys Acta Source Type: research
Preparation of PEGylated cationic nanoliposome-siRNA complexes for cancer therapy.
In conclusion, our novel PEGylated liposomes have high potential for systemic delivery of siRNA and can improve in vivo stability of free siRNA and also siRNA lipoplexes. PMID: 29475393 [PubMed - as supplied by publisher]
Source: Artificial Cells, Nanomedicine and Biotechnology - February 26, 2018 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research
A dual targeting drug delivery system for penetrating blood brain barrier and selectively delivering siRNA to neurons for Alzheimer's disease treatment.
CONCLUSION: In conclusion, this study preliminarily demonstrated the ApoA-I and NL4 dual modified dendrimer nanoparticles were efficient carrier for siRNA delivery to AD bearing brain. PMID: 29484985 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Biotechnology - February 26, 2018 Category: Biotechnology Authors: Zhang C, Gu Z, Shen L, Liu X, Lin H Tags: Curr Pharm Biotechnol Source Type: research
i-Fect Delivers Again and Again
This study revealed that LDHA, which is a known damage marker, promotes CNS-specific angiogenesis. LDHA-mediated angiogenesis depends on vimentin on the surface of vascular endothelial cells. The work described here proposes a novel mechanism by which neurodegeneration drives angiogenesis in the CNS.A mixture of ouri-FectTM and LDHA siRNA, in this study, were directly injected into mice cortexes: Hsiaoyun Lin, Rieko Muramatsu, Noriko Maedera, Hiroto Tsunematsu, Machika Hamaguchi, Yoshihisa Koyama, Mariko Kuroda, Kenji Ono, Makoto Sawada, Toshihide Yamashita.Extracellular Lactate Dehydrogenase A Release ...
Source: Neuromics - February 19, 2018 Category: Neuroscience Tags: angiogenesis Delivering siRNA to the CNS Lactate Hydrogenase A LDHA Neurodegenerative Disease siRNA delivery in-vivo Source Type: news
Development of siRNA mediated RNA interference and functional analysis of novel parasitic nematode-specific protein of Setaria digitata.
In this study, we developed an effective siRNA delivery method by microinjection and utilized the siRNAi tool to knockdown SDNP to study the phenotypic and cellular changes associated with the interference. qPCR analysis revealed, a significant reduction of SDNP transcript levels following siRNA microinjection into S. digitata adult worms. Similarly, immunohistochemical staining indicated a reduction of SDNP protein expression. Furthermore, worms treated with siRNA showed a significant reduction of microfilariae release together with embryonic lethality by arresting an early developmental stage compared to non-treated worm...
Source: Experimental Parasitology - February 12, 2018 Category: Parasitology Authors: Somarathne MBCL, Gunawardena YINS, Chandrasekharan NV, Dassanayake RS Tags: Exp Parasitol Source Type: research
Exosomes serve as nanoparticles to suppress tumor growth and angiogenesis in gastric cancer by delivering hepatocyte growth factor siRNA
Exosomes derived from cells have been found to mediate signal transduction between cells and to act as efficient carriers to deliver drugs and small RNA. Hepatocyte growth factor (HGF) is known to promote the growth of both cancer cells and vascular cells, and the HGF‐cMET pathway is a potential clinical target. Here, we characterized the inhibitory effect of HGF siRNA on tumor growth and angiogenesis in gastric cancer. In addition, we showed that HGF siRNA packed in exosomes can be transported into cancer cells, where it dramatically downregulates HGF expression. A cell co‐culture model was used to show that exosomes ...
Source: Cancer Science - February 11, 2018 Category: Cancer & Oncology Authors: Haiyang Zhang, Yi Wang, Ming Bai, Junyi Wang, Kegan Zhu, Rui Liu, Shaohua Ge, JiaLu Li, Tao Ning, Ting Deng, Qian Fan, Hongli Li, Wu Sun, Guoguang Ying, Yi Ba Tags: ORIGINAL ARTICLE Source Type: research
Effects of Grb2-associated binding protein 2-specific siRNA on the migration and invasion of MG-63 osteosarcoma cells.
Authors: Wang H, He H, Meng H, Cui Y, Wang W Abstract To investigate the association between the expression of growth factor receptor binding protein 2-associated binding protein 2 (Gab2) in human osteosarcoma as well as the effects of Gab2 on invasion and metastasis, human MG-63 osteosarcoma cells were transfected with small interfering (si)RNA plasmid. Gab2 protein and mRNA expression levels were detected using western blotting and reverse transcription-polymerase chain reaction, respectively. The cell migration and invasion abilities were detected using in vitro chemotaxis and invasion assays, respectively, foll...
Source: Oncology Letters - February 10, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
Engineering functional inorganic-organic hybrid systems: advances in siRNA therapeutics
Chem. Soc. Rev., 2018, Advance Article DOI: 10.1039/C7CS00479F, Review ArticleJianliang Shen, Wei Zhang, Ruogu Qi, Zong-Wan Mao, Haifa Shen Engineering functional inorganic-organic hybrid vectors for siRNA delivery systems as a promising approach to overcome many physiological and biological obstacles, which greatly paves the way for a successful, safe and efficient platform in clinical applications. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chemical Society Reviews - February 8, 2018 Category: Chemistry Authors: Jianliang Shen Source Type: research
Genes, Vol. 9, Pages 74: Covalent Strategies for Targeting Messenger and Non-Coding RNAs: An Updated Review on siRNA, miRNA and antimiR Conjugates
Eritja Oligonucleotide-based therapy has become an alternative to classical approaches in the search of novel therapeutics involving gene-related diseases. Several mechanisms have been described in which demonstrate the pivotal role of oligonucleotide for modulating gene expression. Antisense oligonucleotides (ASOs) and more recently siRNAs and miRNAs have made important contributions either in reducing aberrant protein levels by sequence-specific targeting messenger RNAs (mRNAs) or restoring the anomalous levels of non-coding RNAs (ncRNAs) that are involved in a good number of diseases including cancer. In addition t...
Source: Genes - February 6, 2018 Category: Genetics & Stem Cells Authors: Santiago Grijalvo Adele Alagia Andreia Jorge Ramon Eritja Tags: Review Source Type: research
Nanotechnology-Based Strategies for siRNA Brain Delivery for Disease Therapy.
Abstract Small interfering RNA (siRNA)-based gene silencing technology has demonstrated significant potential for treating brain-associated diseases. However, effective and safe systemic delivery of siRNA into the brain remains challenging because of biological barriers such as enzymatic degradation, short circulation lifetime, the blood-brain barrier (BBB), insufficient tissue penetration, cell endocytosis, and cytosolic transport. Nanotechnology offers intriguing potential for addressing these challenges in siRNA brain delivery in conjunction with chemical and biological modification strategies. In this review, ...
Source: Trends in Biotechnology - February 5, 2018 Category: Biotechnology Authors: Zheng M, Tao W, Zou Y, Farokhzad OC, Shi B Tags: Trends Biotechnol Source Type: research
PARP1-siRNA suppresses human prostate cancer cell growth and progression.
Authors: Lai Y, Kong Z, Zeng T, Xu S, Duan X, Li S, Cai C, Zhao Z, Wu W Abstract Poly (ADP-ribose) polymerase (PARP) inhibitors, such as olaparib or rucaparib, have shown treatment efficacy in BRCA1/2-deficient tumors. However, since PARP inhibitors (PARPi) mainly modulate the activation of PARP but not its expression, whether small interfering RNA (siRNA) specific to PARP has the same function as PARPi has not been well defined. In the present study it was demonstrated that PARP1-siRNA could reduce prostate cancer (PCa) cell progression regardless of the BRCA1/2 mutation. PARP1 silencing could significantly inhibi...
Source: Oncology Reports - February 4, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Noncovalent tagging of siRNA with steroids for transmembrane delivery.
Abstract Short interfering RNA (siRNA) has broad applications in biology and medicine, and holds tremendous potential to become a new class of therapeutics for many diseases. As a highly anionic macrobiomolecule, its cytosolic delivery, however, has been a major roadblock in translation. Here, we report the development of small, bifunctional chemical tags capable of transporting siRNA directly into the cytosol. The bifunctional tags consist of a siRNA-binding moiety that interacts with siRNA non-covalently, and a steroid domain that readily fuses with the mammalian cell membrane. In contrast to the conventional co...
Source: Biomaterials - February 3, 2018 Category: Materials Science Authors: Tai W, Gao X Tags: Biomaterials Source Type: research
Dual-responsive polyplexes with enhanced disassembly and endosomal escape for efficient delivery of siRNA.
Abstract Despite the extracellular barriers for siRNA delivery have been overcome by utilizing advanced nanoparticle delivery systems, the key intracellular barriers after internalization including efficient disassembly of siRNA and endosomal escape still remains challenging. To address the issues, we developed a unique pH- and redox potential-responsive polyplex delivery system based on the copolymer of mPEG-b-PLA-PHis-ssPEI1.8 k, which is composed of a pH-responsive copolymer of PEG-b-PLA-PHis (Mw 5 k) and a branched PEI (Mw1.8 k) linked with redox cleavable disulfide bond. The copolymer showed excellent s...
Source: Biomaterials - February 3, 2018 Category: Materials Science Authors: Zhu J, Qiao M, Wang Q, Ye Y, Ba S, Ma J, Hu H, Zhao X, Chen D Tags: Biomaterials Source Type: research
Recent advances in siRNA delivery for cancer therapy using smart nanocarriers.
Abstract Small interfering RNAs (siRNAs) can selectively target and downregulate disease-causing genes, holding great promise in treating human diseases, especially malignant cancers. However, how to efficiently deliver siRNAs into target cell cytosol is a problem that has hindered their clinical application. Here, we review the recent strategies for siRNA delivery on the basis of smart nanocarriers by using stimuli-responsive materials. We highlight the rationales of how to design smart nanocarriers responsive to physiological and external stimuli to improve the delivery efficiency, targeting precision and gene s...
Source: Drug Discovery Today - January 23, 2018 Category: Drugs & Pharmacology Authors: Zhang P, An K, Duan X, Xu H, Li F, Xu F Tags: Drug Discov Today Source Type: research
GSE93608 Comparison of HBO1 siRNA-treated Human Umbilical Vein Endothelial Cells (HUVECs) and control siRNA-treated HUVECs
Contributors : Philip A Marsden ; Matthew S YanSeries Type : Expression profiling by array ; Non-coding RNA profiling by arrayOrganism : Homo sapiensTo determine the role of HBO1 in EC physiology, gene expression analysis was conducted on control and HBO1 siRNA-treated HUVECs. A total of 263 differentially regulated protein-coding transcripts were detected, many of which are key for growth and angiogenesis. Additionally, many genes involved in cell cycle, cell division, and DNA replication were dysregulated. HBO1-regulated genes were verified by qRT-PCR, including those with roles in vessel tone regulation (e.g. ACE1), ves...
Source: GEO: Gene Expression Omnibus - January 19, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Non-coding RNA profiling by array Homo sapiens Source Type: research
Cyclam-Modified PEI for Combined VEGF siRNA Silencing and CXCR4 Inhibition To Treat Metastatic Breast Cancer.
Abstract Chemokine receptor CXCR4 plays an important role in cancer cell invasion and metastasis. Recent findings suggest that anti-VEGF therapies upregulate CXCR4 expression, which contributes to resistance to antiangiogenic therapies. Here, we report the development of novel derivatives of polyethylenimine (PEI) that effectively inhibit CXCR4 while delivering anti-VEGF siRNA. PEI was alkylated with different amounts of a CXCR4-binding cyclam derivative to prepare PEI-C. Modification with the cyclam derivatives resulted in a considerable decrease in cytotoxicity when compared with unmodified PEI. All the PEI-C sh...
Source: Biomacromolecules - January 19, 2018 Category: Biochemistry Authors: Zhou Y, Yu F, Zhang F, Chen G, Wang K, Sun M, Li J, Oupický D Tags: Biomacromolecules Source Type: research
Potential Molecular Targets in the Treatment of Lung Cancer Using siRNA Technology.
This article dedicated to an update review of molecular targets could potentially be used for target therapy of lung cancer using SiRNA technology. PMID: 29336624 [PubMed - as supplied by publisher]
Source: Cancer Investigation - January 18, 2018 Category: Cancer & Oncology Tags: Cancer Invest Source Type: research
Highly Stable RNA Capture by Dense Cationic Polymer Brushes for the Design of Cytocompatible, Serum-Stable SiRNA Delivery Vectors.
Abstract The high density of polymer brushes confers to these coatings unique physico-chemical properties, in particular for the regulation of biomolecular interaction and the design of highly selective coatings for biosensors and protein patterning. Here we show that high density poly(dimethylaminoethyl methacrylate) cationic polymer brushes enable the stable uptake of high levels of oligonucleotides. This is proposed to result from the high degree of crowding and associated increase in entropic driving force for the binding of polyelectrolytes such as nucleic acid molecules. We further demonstrate the ease with ...
Source: Biomacromolecules - January 16, 2018 Category: Biochemistry Authors: Li D, Sharili AS, Connelly J, Gautrot JE Tags: Biomacromolecules Source Type: research
The proteoglycan biglycan mediates inflammatory response by activating TLR-4 in human chondrocytes: Inhibition by specific siRNA and high polymerized Hyaluronan.
Abstract Cartilage degeneration are hallmarks of wear, tear, mechanical and inflammatory damage of the joint cartilage. Tissue degradation as well as compromising the integrity and function of the organ, produces different intermediates, directly able to stimulate further inflammatory effect, therefore, amplifying the inflammation response. Biglycan is a soluble component of the extracellular matrix that is released during tissue injury. It has been reported that released biglycan is an endogenous ligand for TLR-2/4 in some cell type. We studied the role of biglycan in an experimental model of biglycan-induced inf...
Source: Archives of Biochemistry and Biophysics - January 12, 2018 Category: Biochemistry Authors: Avenoso A, D'Ascola A, Scuruchi M, Mandraffino G, Calatroni A, Saitta A, Campo S, Campo GM Tags: Arch Biochem Biophys Source Type: research