Quantitative analysis of protein crotonylation identifies its association with immunoglobulin A nephropathy.
Authors: Lin H, Tang D, Xu Y, Zhang R, Ou M, Zheng F, Chen J, Zhang Y, Zou G, Xue W, Zou Y, Dai W, Sui W, Dai Y Abstract Posttranslational modifications (PTMs) to histones such as lysine crotonylation are classified as epigenetic changes. Lysine crotonylation participates in various cellular processes and occurs in active promoters, directly accelerating transcription. The present study performed a proteomics analysis of crotonylation between healthy controls and patients with immunoglobulin A (IgA) nephropathy using tandem mass spectrometry and high‑resolution liquid chromatography. The present results identifie...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Construction of a prognosis ‑associated long noncoding RNA‑mRNA network for multiple myeloma based on microarray and bioinformatics analysis.
Construction of a prognosis‑associated long noncoding RNA‑mRNA network for multiple myeloma based on microarray and bioinformatics analysis. Mol Med Rep. 2020 Jan 13;: Authors: Zhu FX, Wang XT, Ye ZZ, Gan ZP, Lai YR Abstract At present, the association between prognosis‑associated long noncoding RNAs (lncRNAs) and mRNAs is yet to be reported in multiple myeloma (MM). The aim of the present study was to construct prognostic models with lncRNAs and mRNAs, and to map the interactions between these lncRNAs and mRNAs in MM. LncRNA and mRNA data from 559 patients with MM were acquired from the Gen...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

miR ‑21 promotes osteoclastogenesis through activation of PI3K/Akt signaling by targeting Pten in RAW264.7 cells.
In conclusion, miR‑21 is upregulated during osteoclastogenesis, and may promote osteoclastogenesis and bone resorption through activating the PI3K/Akt signaling pathway via targeting Pten. PMID: 32016444 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Dexmedetomidine alleviates cisplatin ‑induced acute kidney injury by attenuating endoplasmic reticulum stress‑induced apoptosis via the α2AR/PI3K/AKT pathway.
In conclusion, Dex may alleviate CP‑induced AKI by attenuating ERS‑induced apoptosis, at least in part, via the α2AR/PI3K/AKT signaling pathway. PMID: 32016445 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation.
Authors: Brązert M, Kranc W, Nawrocki MJ, Sujka-Kordowska P, Konwerska A, Jankowski M, Kocherova I, Celichowski P, Jeseta M, Ożegowska K, Antosik P, Bukowska D, Skowroński MT, Bruska M, Pawelczyk L, Zabel M, Piotrowska-Kempisty H, Nowicki M, Kempisty B Abstract Oocyte maturation is essential for proper fertilization, embryo implantation and early development. While the physiological conditions of these processes are relatively well‑known, its exact molecular mechanisms remain widely undiscovered. Oocyte growth, differentiation and maturation are therefore the subject of scientific debate. Precious literature h...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

A six ‑gene support vector machine classifier contributes to the diagnosis of pediatric septic shock.
A six‑gene support vector machine classifier contributes to the diagnosis of pediatric septic shock. Mol Med Rep. 2020 Jan 23;: Authors: Long G, Yang C Abstract Septic shock is induced by an uncontrolled inflammatory immune response to pathogens and the survival rate of patients with pediatric septic shock (PSS) is particularly low, with a mortality rate of 25‑50%. The present study explored the mechanisms of PSS using four microarray datasets (GSE26378, GSE26440, GSE13904 and GSE4607) that were obtained from the Gene Expression Omnibus database. Based on the MetaDE package, the consistently differ...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Ginsenosides reduce body weight and ameliorate hepatic steatosis in high fat diet ‑induced obese mice via endoplasmic reticulum stress and p‑STAT3/STAT3 signaling.
In conclusion, present studies suggest that GS exert these effects by increasing STAT3 phosphorylation expression and reducing the ERS. Thus, GS reduce body weight and ameliorate hepatic steatosis in HFD‑induced obese mice. PMID: 32016448 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Identification of microRNA ‑363‑3p as an essential regulator of chondrocyte apoptosis in osteoarthritis by targeting NRF1 through the p53‑signaling pathway.
Identification of microRNA‑363‑3p as an essential regulator of chondrocyte apoptosis in osteoarthritis by targeting NRF1 through the p53‑signaling pathway. Mol Med Rep. 2020 Jan 14;: Authors: Zhang M, Wang Z, Li B, Sun F, Chen A, Gong M Abstract Osteoarthritis (OA) is a degenerative joint disease that affects the physical, and mental health of middle‑aged and elderly people. The aims of the present study were to determine the biological function and molecular mechanisms of miR‑363‑3p in chondrocyte apoptosis. Exploration of the molecular mechanisms of OA may be helpful in the understand of ...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Silencing of RIPK4 inhibits epithelial ‑mesenchymal transition by inactivating the Wnt/β‑catenin signaling pathway in osteosarcoma.
Silencing of RIPK4 inhibits epithelial‑mesenchymal transition by inactivating the Wnt/β‑catenin signaling pathway in osteosarcoma. Mol Med Rep. 2020 Jan 14;: Authors: Yi Z, Pu Y, Gou R, Chen Y, Ren X, Liu W, Dong P Abstract Receptor interacting protein kinase 4 (RIPK4) is a serine/threonine kinase that plays an important role in the regulation of cell proliferation, invasion and metastasis in several malignancies; however, its clinical significance and biological function in osteosarcoma (OS) remains unknown. In the present study, the RIPK4 expression level was significantly upregulated i...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Oral intake of α‑glucosyl‑hesperidin ameliorates selenite‑induced cataract formation.
Oral intake of α‑glucosyl‑hesperidin ameliorates selenite‑induced cataract formation. Mol Med Rep. 2020 Jan 14;: Authors: Nakazawa Y, Aoki M, Ishiwa S, Morishita N, Endo S, Nagai N, Yamamoto N, Funakoshi-Tago M, Tamura H Abstract Hesperetin is a natural flavonoid with robust antioxidant properties. Our previous study reported that hesperetin can prevent cataract formation. However, an important consideration regarding hesperetin consumption is the limited bioavailability due to its poor solubility. The present study investigated the anti‑cataract effects of α‑glucosyl hesperidin i...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3T3 ‑L1 cells via peroxisome proliferator‑activated receptor γ activation.
Indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3T3‑L1 cells via peroxisome proliferator‑activated receptor γ activation. Mol Med Rep. 2020 Jan 16;: Authors: Konno T, Sasaki K, Kobayashi K, Murata T Abstract The peroxisome proliferator‑activated receptor γ (PPARγ) plays an important role in insulin sensitivity and adipocyte differentiation. It is known as ligand‑receptor that improves insulin sensitivity in type 2 diabetes mellitus. Several kinds of indigo plant have been already used to treat diabetes in oriental traditional medicine, ...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Laminin α5 modulates fibroblast proliferation in epidural fibrosis through the PI3K/AKT/mTOR signaling pathway.
In conclusion, laminin α5 could modulate fibroblast proliferation in epidural fibrosis through the PI3K/AKT/mTOR signaling pathway. PMID: 32016453 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Development of an Ewing sarcoma cell line with resistance to EWS ‑FLI1 inhibitor YK‑4‑279.
Development of an Ewing sarcoma cell line with resistance to EWS‑FLI1 inhibitor YK‑4‑279. Mol Med Rep. 2020 Jan 16;: Authors: Conn E, Hour S, Allegakoen D, Graham G, Petro J, Kouassi-Brou M, Hong SH, Selvanathan S, Çelik H, Toretsky J, Üren A Abstract Despite Ewing sarcoma (ES) being the second most common pediatric malignancy of bone and soft tissue, few novel therapeutic approaches have been introduced over the past few decades. ES contains a pathognomonic chromosomal translocation that leads to a fusion protein between EWSR1 and an ets family member, most often FLI1. EWS‑FLI1 is ...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Long non ‑coding RNA BCYRN1 promotes glycolysis and tumor progression by regulating the miR‑149/PKM2 axis in non‑small‑cell lung cancer.
Long non‑coding RNA BCYRN1 promotes glycolysis and tumor progression by regulating the miR‑149/PKM2 axis in non‑small‑cell lung cancer. Mol Med Rep. 2020 Jan 16;: Authors: Lang N, Wang C, Zhao J, Shi F, Wu T, Cao H Abstract Cancer cells use aerobic glycolysis to sustain their proliferation. Long non‑coding RNA brain cytoplasmic RNA 1 (BCYRN1) has been reported to act as an oncogene in non‑small‑cell lung cancer (NSCLC). The present study investigated the role of BCYRN1 in NSCLC glycolysis. BCYRN1 expression was detected in NSCLC cells and tissues using reverse transcription‑quantitative...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

The peptide compound urantide regulates collagen metabolism in atherosclerotic rat hearts and inhibits the JAK2/STAT3 pathway.
In conclusion, urantide was able to effectively block the UII/GPR14 system by regulating the JAK2/STAT3 pathway and collagen metabolism. Inhibition of the UII/GPR14 system may prevent and potentially treat atherosclerotic myocardial fibrosis. Based on the current results, it was hypothesized that collagen metabolism may be associated with the JAK2/STAT3 pathway. PMID: 32016456 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Integrative analysis of an lncRNA ‑associated competing endogenous RNA network in human trabecular meshwork cells under oxidative stress.
Integrative analysis of an lncRNA‑associated competing endogenous RNA network in human trabecular meshwork cells under oxidative stress. Mol Med Rep. 2020 Jan 21;: Authors: Yao K, Yu Y, Li F, Jin P, Deng C, Zhang H Abstract Long non‑coding RNAs (lncRNAs) are a group of non‑coding transcripts of>200 nucleotides. They can act as competing endogenous RNAs (ceRNAs) and suppress microRNA (miRNA) function by preventing them from binding to and interacting with target mRNAs. However, the specific role of the lncRNA‑associated ceRNA network in the pathogenesis of glaucoma has not yet been elucidated...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Roles of inflammation factors in melanogenesis (Review).
Authors: Fu C, Chen J, Lu J, Yi L, Tong X, Kang L, Pei S, Ouyang Y, Jiang L, Ding Y, Zhao X, Li S, Yang Y, Huang J, Zeng Q Abstract The occurrence of hyperpigmentation or hypopigmentation after inflammation is a common condition in dermatology and cosmetology. Since the exact mechanism of its occurrence is not yet known, prevention and treatment are troublesome. Previous studies have confirmed that α‑melanocyte‑stimulating hormone, stem cell factor and other factors can promote melanogenesis‑related gene expression through the activation of signaling pathways. Recent studies have revealed that a variety...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Lithium chloride increases sensitivity to photon irradiation treatment in primary mesenchymal colon cancer cells.
Authors: Cammarota F, Conte A, Aversano A, Muto P, Ametrano G, Riccio P, Turano M, Valente V, Delrio P, Izzo P, Pierantoni GM, De Rosa M Abstract Colorectal cancer (CRC) is the third most prevalent type of cancer worldwide. It is also the second most common cause of cancer‑associated mortality; it accounted for about 9.2% of all cancer deaths in 2018, most of which were due to resistance to therapy. The main treatment for CRC is surgery, generally associated with chemotherapy, radiation therapy and combination therapy. However, while chemo‑radiotherapy kills differentiated cancer cells, mesenchymal stem‑like ...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

MicroRNA ‑27a‑3p promotes epithelial‑mesenchymal transition by targeting NOVA alternative splicing regulator 1 in gastric cancer.
MicroRNA‑27a‑3p promotes epithelial‑mesenchymal transition by targeting NOVA alternative splicing regulator 1 in gastric cancer. Mol Med Rep. 2020 Jan 17;: Authors: Li K, Zhu X, Chen X, Wang X Abstract NOVA alternative splicing regulator 1 (NOVA1) dysregulation has been detected in the gastric cancer microenvironment. Decreased NOVA1 expression has been linked to the progression and poor prognosis of gastric cancer; however, the role of NOVA1 in regulating epithelial‑mesenchymal transition (EMT) remains unclear in this disease. Experimental evidence has shown that miR‑27a‑3p ...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Icariin promotes osteogenic differentiation of BMSCs by upregulating BMAL1 expression via BMP signaling.
In conclusion, these results demonstrated that icariin promoted osteogenic differentiation through BMAL1‑BMP2 signaling in BMSCs. The present study thus described a novel target of icariin that has potential applications in the treatment of osteogenic disorders. PMID: 32016461 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Propofol inhibits proliferation and cisplatin resistance in ovarian cancer cells through regulating the microRNA ‑374a/forkhead box O1 signaling axis.
In conclusion, propofol downregulated miR‑374a and modulated the FOXO1 pathway to reduce proliferation and DDP resistance in ovarian cancer cells. PMID: 32016462 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Mechanism of the hypoxia inducible factor 1/hypoxic response element pathway in rat myocardial ischemia/diazoxide post ‑conditioning.
Mechanism of the hypoxia inducible factor 1/hypoxic response element pathway in rat myocardial ischemia/diazoxide post‑conditioning. Mol Med Rep. 2020 Jan 28;: Authors: Li J, Zhou W, Chen W, Wang H, Zhang Y, Yu T Abstract Ischemic post‑conditioning (IPO) and diazoxide post‑conditioning (DPO) has been proven to reduce myocardial ischemia reperfusion injury (MIRI); however, the mechanisms of IPO/DPO are still not clear. The present study aimed to investigate whether mitochondrial ATP‑sensitive potassium channels (mitoKATP) channels are activated by IPO/DPO, which may further activate the hyp...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Schwann cell ‑derived exosomes induce bone marrow‑derived mesenchymal stem cells to express Schwann cell markers in vitro.
In conclusion, Schwann cell‑derived exosomes may be involved in the differentiation of BMSCs into Schwann cells, which may provide a novel target for promoting nerve regeneration following injury. PMID: 32016464 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Low ‑intensity ultrasound enhances the antitumor effects of doxorubicin on hepatocellular carcinoma cells through the ROS‑miR‑21‑PTEN axis.
Low‑intensity ultrasound enhances the antitumor effects of doxorubicin on hepatocellular carcinoma cells through the ROS‑miR‑21‑PTEN axis. Mol Med Rep. 2020 Jan 13;: Authors: Xia C, Zeng H, Zheng Y Abstract Hepatocellular carcinoma (HCC) is a type of liver cancer and is a leading cause of cancer‑associated mortality. In China, ~466,000 patients are diagnosed with HCC and it is responsible for ~422,000 cases of mortality each year. Surgery is the most effective treatment available; however it is only suitable for patients with early‑stage HCC. Chemotherapy has been confirmed as a n...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Iron deficiency attenuates catecholamine ‑stimulated lipolysis via downregulation of lipolysis‑related proteins and glucose utilization in 3T3‑L1 adipocytes.
In conclusion, the results indicated that low iron availability attenuated catecholamine‑stimulated lipolysis by downregulating lipolytic enzymes and glucose utilization in 3T3‑L1 adipocytes. PMID: 32016466 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

MicroRNA ‑628‑5p inhibits cell proliferation and induces apoptosis in colorectal cancer through downregulating CCND1 expression levels.
In conclusion, the present study suggested that colorectal cancer progression may be regulated through the miR‑628‑5p/CCND1 axis, and miR‑628‑5p could be used as a potential diagnostic and prognostic biomarker for colorectal cancer. PMID: 32016467 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Impact of a high ‑fat diet on intestinal stem cells and epithelial barrier function in middle‑aged female mice.
Impact of a high‑fat diet on intestinal stem cells and epithelial barrier function in middle‑aged female mice. Mol Med Rep. 2020 Jan 13;: Authors: Xie Y, Ding F, Di W, Lv Y, Xia F, Sheng Y, Yu J, Ding G Abstract A high‑fat diet (HFD) or obesity‑promoting diet is closely associated with metabolic diseases and intestinal tumors, particularly in middle‑aged individuals (typically 45‑64 years old). The intestinal epithelium constitutes a barrier that separates the host from the food and microbiota in the gut, and thus, a dysfunctional epithelium is associated with a number of diseases. How...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Pretreatment with dexmedetomidine alleviates lung injury in a rat model of intestinal ischemia reperfusion.
Authors: Chen Y, Bian W, Xu B Abstract The aim of the present study was to investigate the antioxidant mechanisms of dexmedetomidine against lung injury during intestinal ischemia reperfusion (IIR) in rats. The model of IIR‑induced acute lung injury was established by occluding the superior mesenteric artery (SMA) for 1 h and reperfusing for 2 h using Sprague‑Dawley rats. Pathological examination was used to assess the extent of the lung injury. Oxidative stress was evaluated by measuring malondialdehyde, myeloperoxidase and superoxide dismutase in the lung and plasma. The proinflammatory cytokines tu...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Sialylation of FGFR1 by ST6Gal ‑I overexpression contributes to ovarian cancer cell migration and chemoresistance.
Sialylation of FGFR1 by ST6Gal‑I overexpression contributes to ovarian cancer cell migration and chemoresistance. Mol Med Rep. 2020 Jan 20;: Authors: Ou L, He X, Liu N, Song Y, Li J, Gao L, Huang X, Deng Z, Wang X, Lin S Abstract Fibroblast growth factor receptors (FGFRs) have been implicated in the malignant transformation and chemoresistance of epithelial ovarian cancer; however, the underlying molecular mechanisms are poorly understood. Increased sialyltransferase activity that enhances protein sialylation is an important post‑translational process promoting cancer progression and malignancy. In...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Resveratrol attenuates IL ‑33‑induced mast cell inflammation associated with inhibition of NF‑κB activation and the P38 signaling pathway.
Resveratrol attenuates IL‑33‑induced mast cell inflammation associated with inhibition of NF‑κB activation and the P38 signaling pathway. Mol Med Rep. 2020 Jan 20;: Authors: Xu Y, Liu Q, Guo X, Xiang L, Zhao G Abstract Resveratrol (RSV), a natural polyphenol found in grapes and other herbal plants, has been reported to possess anti‑inflammatory, anti‑oxidative and anti‑proliferative activities. The aim of the present study was to investigate the effect of RSV on interleukin (IL)‑33‑induced inflammatory responses in mast cells and identify the underlying molecular mechanisms. Rat b...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Role of downregulated ADARB1 in lung squamous cell carcinoma.
Authors: Wang X, Ren X, Liu W, Chen X, Wei J, Gong Z, Yan Y, Xu Z Abstract Non‑small cell lung cancer (NSCLC) is prevalent worldwide. Lung squamous cell carcinoma (LUSC) is one of the main subtypes of NSCLC yet, currently, few biomarkers are available for the diagnosis of LUSC. The present study aimed to investigate the expression and role of adenosine deaminase RNA specific B1 (ADARB1) in lung squamous cell carcinoma (LUSC). Integrative bioinformatics analysis was used to identify the effects of ADARB1 expression on the occurrence and prognosis of LUSC. The expression of ADARB1 was further examined by immunohist...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model.
The objective of the present study was to investigate the changes in the redox system associated with HBO therapy maintained during the first hour after coronary occlusion in an acute myocardial infarction (MI) rat model. Surviving male rats (n=105) were randomly assigned to one of three groups: Sham (SH=26), myocardial infarction (MI=45) and infarction+hyperbaric therapy (HBO=34, 1 h at 2.5 atm). After 90 min of coronary occlusion, a sample of the heart was collected for western blot analysis of total protein levels of superoxide dismutase, catalase, peroxiredoxin and 3‑nitrotyrosine. Glutathione was meas...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Identification of HeLa cell proteins that interact with Chlamydia  trachomatis glycogen synthase using yeast two‑hybrid assays.
Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays. Mol Med Rep. 2020 Jan 16;: Authors: Sun Z, Sun Y, Li Y, Luan X, Chen H, Wu H, Peng B, Lu C Abstract Chlamydia trachomatis (C. trachomatis) is the leading cause of bacterial sexually transmitted diseases and infectious diseases that cause blindness. The pathophysiology of chlamydial infections is poorly understood, but secreted proteins have emerged as key virulence factors. C. trachomatis glycogen synthase (GlgA) is a chlamydial secretory protein, which lo...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Protective effect of GYY4137, a water ‑soluble hydrogen sulfide‑releasing molecule, on intestinal ischemia‑reperfusion.
Protective effect of GYY4137, a water‑soluble hydrogen sulfide‑releasing molecule, on intestinal ischemia‑reperfusion. Mol Med Rep. 2020 Jan 24;: Authors: Cui N, Luo H, Zhao Y Abstract The present study aimed to clarify the protective effects of p‑methoxyphenyl morpholino‑phosphinodithioic acid (GYY4137), a water‑soluble hydrogen sulfide‑releasing molecule, on a rat model of intestinal ischemia‑reperfusion (IIR). A total of 40 healthy male Sprague Dawley (SD) rats were randomly divided into four groups (n=10/group): Group A, a sham‑surgery group; Group B, the IIR group; group C, rats ...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Identifying potential prognostic biomarkers in head and neck cancer based on the analysis of microRNA expression profiles in TCGA database.
Authors: Wang X, Yin Z, Zhao Y, He M, Dong C, Zhong M Abstract The present study aimed to identify sensitive, specific and independent prognostic biomarkers in head and neck cancer (HNC) based on microRNA expression profiles and other high‑throughput sequencing data in The Cancer Genome Atlas (TCGA) database. Identification of such prognostic biomarkers could provide insight into HNC diagnosis and treatment. The differential expression profiles of microRNAs between HNC tissues and adjacent cancer tissues in the TCGA database were analyzed (log fold‑change>2; P
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Methylation ‑driven genes PMPCAP1, SOWAHC and ZNF454 as potential prognostic biomarkers in lung squamous cell carcinoma.
In conclusion, the MDGs PMPCAP1, SOWAHC and ZNF454 may be potential prognostic biomarkers of LUSC for guiding diagnosis and therapy options, as well as providing a theoretical basis for further investigation. PMID: 32016477 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Protective effect of microRNA ‑381 against inflammatory damage of endothelial cells during coronary heart disease by targeting CXCR4.
Protective effect of microRNA‑381 against inflammatory damage of endothelial cells during coronary heart disease by targeting CXCR4. Mol Med Rep. 2020 Jan 22;: Authors: Li Y, Huang J, Yan H, Li X, Ding C, Wang Q, Lu Z Abstract Coronary heart disease (CHD) is the leading cause of human morbidity and mortality worldwide. MicroRNA (miRNA) profiling is an innovative method of identifying biomarkers for many diseases and may be a powerful tool in the diagnosis and treatment of CHD. The present study aimed to analyze the effects of miRNA (miR)‑381 on the inflammatory damage of endothelial cells during CH...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Use of Bao Gui capsule in treatment of a polycystic ovary syndrome rat model.
Authors: Lian Y, Zhao F, Wang W Abstract The present study was designed to elucidate the underlying mechanisms of Bao Gui capsule (BGC) against hyperandrogenism, insulin resistance and leptin resistance of PCOS. Letrozole was used to induce a PCOS model in rats, which were then randomly divided into four groups (n=9): Control, Model, high‑dose BGC (BGC‑H) and low‑dose BGC (BGC‑L) group. Serum levels of follicle‑stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), estradiol (E2), insulin, leptin, and interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α (TNF‑α) in...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Anti ‑metastatic effects of arctigenin are regulated by MAPK/AP‑1 signaling in 4T‑1 mouse breast cancer cells.
Anti‑metastatic effects of arctigenin are regulated by MAPK/AP‑1 signaling in 4T‑1 mouse breast cancer cells. Mol Med Rep. 2020 Jan 13;: Authors: Lee MG, Lee KS, Nam KS Abstract Arctigenin is a natural lignan that is found in burdock with anti‑viral, ‑oxidative, ‑inflammatory and anti‑tumor activities. In the current study, the effect of arctigenin on metastatic potential was examined in 4T‑1 mouse triple‑negative breast cancer cells. The results indicated that arctigenin inhibited cell motility and invasiveness, which was determined using wound healing and transwell invasion assays. ...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

SP1/TGF ‑β1/SMAD2 pathway is involved in angiogenesis during osteogenesis.
In conclusion, it was demonstrated that SP1 promoted TGF‑β1 expression, activated the SMAD2 pathway and induced VEGF secretion, which may enhance angiogenic processes in preosteoblasts. PMID: 32016481 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Monocyte chemoattractant protein ‑1 promotes the proliferation, migration and differentiation potential of fibroblast‑like synoviocytes via the PI3K/P38 cellular signaling pathway.
Monocyte chemoattractant protein‑1 promotes the proliferation, migration and differentiation potential of fibroblast‑like synoviocytes via the PI3K/P38 cellular signaling pathway. Mol Med Rep. 2020 Jan 29;: Authors: Tong X, Zeng H, Gu P, Wang K, Zhang H, Lin X Abstract Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation of the joints and joint destruction. Monocyte chemoattractant protein 1 (MCP‑1) is highly expressed in the joints of patients suffering from RA. The present study aimed to evaluate the effects of MCP‑1 on the phenotype of fibroblast‑like sy...
Source: Molecular Medicine Reports - February 7, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Polyphyllin VII induces apoptotic cell death via inhibition of the PI3K/Akt and NF ‑κB pathways in A549 human lung cancer cells.
Polyphyllin VII induces apoptotic cell death via inhibition of the PI3K/Akt and NF‑κB pathways in A549 human lung cancer cells. Mol Med Rep. 2020 Feb;21(2):597-606 Authors: He H, Xu C, Zheng L, Wang K, Jin M, Sun Y, Yue Z Abstract Polyphyllin VII is an active compound isolated from Paris polyphylla, which is termed Chonglou in China. The present study was designed to investigate the underlying mechanisms of the antitumor effect of Polyphyllin VII in lung cancer cells. The cytotoxic effect of Polyphyllin VII in human lung cancer A549 cells was analyzed; the results revealed an IC50 value of 0.41...
Source: Molecular Medicine Reports - January 26, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Comparative proteomic and genomic analyses of Brucella abortus biofilm and planktonic cells.
In conclusion, these differentially expressed genes may play important roles in bacterial defense, colonization, invasion, and virulence. PMID: 31974592 [PubMed - in process] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - January 26, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Low molecular weight heparin (nadroparin) improves placental permeability in rats with gestational diabetes mellitus via reduction of tight junction factors.
In conclusion, GDM was associated with increased placental permeability and this may be linked with changes in TJs. LMWH intervention mediated protection against this GDM‑associated shift in placental permeability via the RAGE/NF‑κB pathway. PMID: 31974593 [PubMed - in process] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - January 26, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Identification of hub genes and key pathways of dietary advanced glycation end products ‑induced non‑alcoholic fatty liver disease by bioinformatics analysis and animal experiments.
Identification of hub genes and key pathways of dietary advanced glycation end products‑induced non‑alcoholic fatty liver disease by bioinformatics analysis and animal experiments. Mol Med Rep. 2020 Feb;21(2):685-694 Authors: Wang J, Liu H, Xie G, Cai W, Xu J Abstract Non‑alcoholic fatty liver disease (NAFLD) is a common chronic liver disease. Advanced glycation end products (AGEs) negatively affect the liver and accelerate NAFLD progression; however, the underlying mechanisms remain unclear. The present study aimed to examine the effect and mechanism of dietary AGEs on the mouse liver using bioi...
Source: Molecular Medicine Reports - January 26, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Synergistic effect of halofuginone and dexamethasone on LPS ‑induced acute lung injury in type II alveolar epithelial cells and a rat model.
Synergistic effect of halofuginone and dexamethasone on LPS‑induced acute lung injury in type II alveolar epithelial cells and a rat model. Mol Med Rep. 2020 Feb;21(2):927-935 Authors: Du HL, Zhai AD, Yu H Abstract Acute lung injury (ALI) is characterized by neutrophilic infiltration, uncontrolled oxidative stress and inflammatory processes. Despite various therapeutic regimes having been performed, there remains no effective pharmacotherapy available to treat ALI. Halofuginone (HF), a ketone isolated from Dichroa febrifuga, exhibits significant anti‑inflammatory and antifibrotic effects. Dexa...
Source: Molecular Medicine Reports - January 26, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Class  C1 decoy oligodeoxynucleotide inhibits profibrotic genes expression in rat hepatic stellate cells.
In conclusion, class C1 decoy ODNs inhibited pro‑fibrotic gene expression in rat HSCS by downregulating TGF‑β signaling. PMID: 31974596 [PubMed - in process] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - January 26, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Effect of the alteration of Tribbles homologue 3 expression on epithelial ‑mesenchymal transition of transforming growth factor β1‑induced mouse alveolar epithelial cells through the Wnt/β‑catenin signaling pathway.
Effect of the alteration of Tribbles homologue 3 expression on epithelial‑mesenchymal transition of transforming growth factor β1‑induced mouse alveolar epithelial cells through the Wnt/β‑catenin signaling pathway. Mol Med Rep. 2020 Feb;21(2):615-622 Authors: Yu W, Mi L, Wang F Abstract The aims of the present study were to elucidate the regulatory effect of exogenous Tribbles homologue 3 (TRB3) expression on the Wnt/β‑catenin signaling pathway and epithelial‑mesenchymal transition (EMT) in transforming growth factor‑β1 (TGF‑β1)‑induced mouse alveolar epithelial...
Source: Molecular Medicine Reports - January 26, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Identification of key genes as potential biomarkers for triple ‑negative breast cancer using integrating genomics analysis.
Identification of key genes as potential biomarkers for triple‑negative breast cancer using integrating genomics analysis. Mol Med Rep. 2020 Feb;21(2):557-566 Authors: Zhong G, Lou W, Shen Q, Yu K, Zheng Y Abstract Triple‑negative breast cancer (TNBC) accounts for the worst prognosis of all types of breast cancers due to a high risk of recurrence and a lack of targeted therapeutic options. Extensive effort is required to identify novel targets for TNBC. In the present study, a robust rank aggregation (RRA) analysis based on genome‑wide gene expression datasets involving TNBC patients from the Gen...
Source: Molecular Medicine Reports - January 26, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Metabolic disparities of different oxidative stress ‑inducing conditions in HTR8/SVneo cells.
In this study, the aim was to characterize the intracellular and extracellular metabolite profiles of different oxidative stress cell models commonly used in the research of pregnancy complications. HTR8/SVneo human trophoblast cell lines were treated with five different oxidative stress‑inducing conditions: Hypoxia (1% oxygen); hypoxia and reoxygenation; cobalt chloride (CoCl2; 300 µmol/l); sodium nitroprusside (SNP; 2.5 mmol/l); and the serum of women with preeclampsia (10% v/v). Intracellular metabolites were extracted from cells and extracellular metabolites were collected from spent media for me...
Source: Molecular Medicine Reports - January 26, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research