Identification of critical genes associated with spinal cord injury based on the gene expression profile of spinal cord tissues from trkB.T1 knockout mice.
Authors: Wei L, He F, Zhang W, Chen W, Yu B Abstract The present study aimed to identify the genes and underlying mechanisms critical to the pathology of spinal cord injury (SCI). Gene expression profiles of spinal cord tissues of trkB.T1 knockout (KO) mice following SCI were accessible from the Gene Expression Omnibus database. Compared with trkB.T1 wild type (WT) mice, the differentially expressed genes (DEGs) in trkB.T1 KO mice following injury at different time points were screened out. The significant DEGs were subjected to function, co‑expression and protein‑protein interaction (PPI) network analyses. A t...
Source: Molecular Medicine Reports - February 14, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Expression and significance of EBV, ARID1A and PIK3CA in gastric carcinoma.
Authors: Zhou H, Tan S, Li H, Lin X Abstract AT‑rich interaction domain 1A (ARID1A) and phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit α (PIK3CA) serve important roles in the formation and development of numerous malignancies including gastric cancer. Accumulating evidence has demonstrated that Epstein‑Barr virus (EBV) is a pathogenic virus associated with gastric cancer. The present study aimed to investigate the association between EBV infection, and the expression levels of ARID1A and PIK3CA in gastric cancer. EBER in situ hybridization was performed to detec...
Source: Molecular Medicine Reports - February 14, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

NLRP3 inflammasome inactivation driven by miR ‑223‑3p reduces tumor growth and increases anticancer immunity in breast cancer.
NLRP3 inflammasome inactivation driven by miR‑223‑3p reduces tumor growth and increases anticancer immunity in breast cancer. Mol Med Rep. 2019 Jan 23;: Authors: Zhang L, Li H, Zang Y, Wang F Abstract MicroRNA‑233‑3p (miR‑223‑3p) is considered an important cancer‑associated marker. The NACHT, LRR and PYD domains‑containing protein 3 (NLRP3) inflammasome represents a novel potential target for the treatment of breast cancer. Therefore, it was hypothesized that miR‑223‑3p may affect tumor growth and immunosuppression in breast cancer by inhibiting the NLRP3 inflammasome. In the presen...
Source: Molecular Medicine Reports - February 14, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Endothelial progenitor cell impairment mediated vasodilation dysfunction via diminishing nitric oxide production in postmenopausal females.
Authors: Wu WZ, Hu DJ, Wang ZY, Liao LS, Li CC Abstract Vascular endothelial dysfunction is the major contributing factor to hypertension. Endothelial progenitor cells (EPCs) are essential for endogenous vascular endothelial renovation. The activity and number of circulating EPCs are preserved in prehypertensive premenopausal females according to our previous research. However, the changes of EPCs in prehypertensive postmenopausal females are poorly understood, and the mechanisms responsible for the loss of the gender protection advantage of cardiovascular disease remain unexplored. In order to determine the effect...
Source: Molecular Medicine Reports - February 14, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

IL ‑6 prevents CXCL8‑induced stimulation of EpCAM expression in ovarian cancer cells.
IL‑6 prevents CXCL8‑induced stimulation of EpCAM expression in ovarian cancer cells. Mol Med Rep. 2019 Jan 23;: Authors: Kapka-Skrzypczak L, Popek S, Sawicki K, Drop B, Czajka M, Jodłowska-Jędrych B, Matysiak-Kucharek M, Furman-Toczek D, Zagórska-Dziok M, Kruszewski M Abstract Epithelial cell adhesion molecule (EpCAM), which is expressed in the majority of epithelial tissues, exhibits tumor growth promoting abilities and is overexpressed in human epithelial ovarian cancer. Therefore, EpCAM is considered to be a promising target for specific immune‑based therapies. The present study evalu...
Source: Molecular Medicine Reports - February 14, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

miRNA ‑135a regulates Hut78 cell proliferation via the GATA‑3/TOX signaling pathway.
miRNA‑135a regulates Hut78 cell proliferation via the GATA‑3/TOX signaling pathway. Mol Med Rep. 2019 Jan 22;: Authors: Wei H, Liu R, Guo X, Zhou Y, Sun B, Wang J Abstract The present study investigated the role of microRNA‑135a (miR‑135a) in cutaneous T‑cell lymphoma (CTCL) proliferation. Compared with the normal T lymphocyte control cell line, the mRNA and protein levels of GATA binding protein 3 (GATA‑3) were markedly increased in the Hut78 cell line and miR‑135a was markedly decreased (P
Source: Molecular Medicine Reports - February 14, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Long noncoding RNA GAS5 promotes apoptosis in primary nucleus pulposus cells derived from the human intervertebral disc via Bcl ‑2 downregulation and caspase‑3 upregulation.
Long noncoding RNA GAS5 promotes apoptosis in primary nucleus pulposus cells derived from the human intervertebral disc via Bcl‑2 downregulation and caspase‑3 upregulation. Mol Med Rep. 2019 Jan 22;: Authors: Wang Y, Song Q, Huang X, Chen Z, Zhang F, Wang K, Huang G, Shen H Abstract Nucleus pulposus cell (NPC) apoptosis serves an important role in intervertebral disc degeneration (IDD); however, the roles of long noncoding RNAs (lncRNAs) in this process remain unknown. The present study aimed to determine the effects of the lncRNA growth arrest‑specific transcript 5 (GAS5) on the apoptosis of pri...
Source: Molecular Medicine Reports - February 14, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Anti ‑inflammatory effects of Dendropanax morbifera in lipopolysaccharide‑stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis.
Anti‑inflammatory effects of Dendropanax morbifera in lipopolysaccharide‑stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis. Mol Med Rep. 2019 Jan 22;: Authors: Choo GS, Lim DP, Kim SM, Yoo ES, Kim SH, Kim CH, Woo JS, Kim HJ, Jung JY Abstract Dendropanax morbifera (D. morbifera), known as Dendro, means 'omnipotent drug' (Panax), and has been called the panacea tree. Various studies on D. morbifera are currently ongoing, aiming to determine its medicinal uses. The present study investigated the anti‑inflammatory effects and underlying mechanism of a nat...
Source: Molecular Medicine Reports - February 14, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Long noncoding RNA homeobox A11 antisense promotes transforming growth factor β1‑induced fibrogenesis in cardiac fibroblasts.
Long noncoding RNA homeobox A11 antisense promotes transforming growth factor β1‑induced fibrogenesis in cardiac fibroblasts. Mol Med Rep. 2019 Jan 24;: Authors: Wang J, Liu X, Zhuang Q, Pan R, Zou L, Cen Z, Tang L Abstract Cardiac fibrosis is closely associated with various heart diseases and is an important pathological feature of cardiac remodeling. However, detailed mechanisms underlying cardiac fibrosis remain largely unknown. Long noncoding RNAs (lncRNAs) are reported to serve significant roles in the development of cardiac fibrosis. The present study aimed to identify the role of a novel ...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Long non ‑coding RNA DANCR promotes nasopharyngeal carcinoma cell proliferation and migration.
Long non‑coding RNA DANCR promotes nasopharyngeal carcinoma cell proliferation and migration. Mol Med Rep. 2019 Jan 28;: Authors: Hao Y, Zhao H, Jin X, He P, Zhang J, Dong Q, Shi W, Zhao M Abstract Aberrant expression of numerous long non‑coding RNAs (lncRNAs) has been reported to be associated with nasopharyngeal carcinoma (NPC). The present study aimed to investigate the expression and function of lncRNA differentiation antagonizing non‑protein coding RNA (DANCR) in NPC pathogenesis. Reverse transcription‑quantitative polymerase chain reaction results suggested that DANCR was significantly up...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Profiling lncRNA alterations during TNF ‑α induced osteogenic differentiation of dental pulp stem cells.
Profiling lncRNA alterations during TNF‑α induced osteogenic differentiation of dental pulp stem cells. Mol Med Rep. 2019 Jan 24;: Authors: Tao R, Li YX, Liu YK, Liu F, Zhou ZY Abstract The multipotent and easily accessible characteristics of dental pulp stem cells (DPSCs) make them a promising target for bone tissue engineering. Long non‑coding RNAs (lncRNAs) have an important role in the osteogenic differentiation of mesenchymal stem cells. Nevertheless, whether lncRNAs are involved in the osteogenic differentiation of DPSCs remains unclear. The present study examined the expression alterat...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Mutation of IDH1 aggravates the fatty acid ‑induced oxidative stress in HCT116 cells by affecting the mitochondrial respiratory chain.
Mutation of IDH1 aggravates the fatty acid‑induced oxidative stress in HCT116 cells by affecting the mitochondrial respiratory chain. Mol Med Rep. 2019 Jan 28;: Authors: Li S, Sun C, Gu Y, Gao X, Zhao Y, Yuan Y, Zhang F, Hu P, Liang W, Cao K, Zhang J, Wang Z, Ye J Abstract Increasing evidence has indicated that mutations of isocitrate dehydrogenase 1/2 (IDH1/2) contribute to the metabolic reprogramming of cancer cells; however their functions in lipid metabolism remain unknown. In the present study, the parental and IDH1 (R132H/+) mutant HCT116 cells were treated with various concentrations of oleic ...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

MicroRNA ‑124 improves functional recovery and suppresses Bax‑dependent apoptosis in rats following spinal cord injury.
MicroRNA‑124 improves functional recovery and suppresses Bax‑dependent apoptosis in rats following spinal cord injury. Mol Med Rep. 2019 Jan 28;: Authors: Xu Z, Zhang K, Wang Q, Zheng Y Abstract Spinal cord injury (SCI) induces aberrant expression of microRNAs (miRNAs), causing various secondary injury responses, including inflammation, apoptosis and oxidative stress. However, the mechanisms underlying miRNA‑mediated apoptosis have not been fully elucidated. In the present study, a rat SCI model was established and a miRNA microarray was analyzed to detect miRNA expression profiles at different t...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620 cells.
Authors: Ju F, Li N, Wang W, Yuan H Abstract Colorectal cancer (CRC), also known as bowel cancer, is one of the leading causes of cancer‑associated mortality worldwide at present. The aim of the present study was to detect the effects of matrix metalloproteinase 1 (MMP1) on the viability and migration of a CRC cell line in the presence or absence of variation X‑ray radiation doses. The CRC cell line, SW620, was cultured and treated with different X‑ray doses (0, 0.1, 0.5, 1, 3 and 6 Gy). MMP1 expression was downregulated via the application of a specific small interfering (si)‑RNA. The viability a...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Expression of TRB3 promotes epithelial ‑mesenchymal transition of MLE‑12 murine alveolar type II epithelial cells through the TGF‑β1/Smad3 signaling pathway.
Expression of TRB3 promotes epithelial‑mesenchymal transition of MLE‑12 murine alveolar type II epithelial cells through the TGF‑β1/Smad3 signaling pathway. Mol Med Rep. 2019 Jan 28;: Authors: Cheng W, Mi L, Tang J, Yu W Abstract The aim of the present study was to investigate whether the expression of tribbles pseudokinase 3 (TRB3) is involved in pulmonary interstitial fibrosis and to examine the possible mechanisms. The expression of TRB3 in murine alveolar type II epithelial cells (MLE‑12 cells) following transforming growth factor β1 (TGF‑β1) stimulation was assessed using ...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Insulin ‑like growth factor I promotes adipogenesis in hemangioma stem cells from infantile hemangiomas.
Insulin‑like growth factor I promotes adipogenesis in hemangioma stem cells from infantile hemangiomas. Mol Med Rep. 2019 Jan 24;: Authors: Wang F, Li H, Lou Y, Xie J, Cao D, Huang X Abstract Infantile hemangiomas (IH) are the most common infantile neoplasms and are characterized by initial proliferation during infancy and subsequent spontaneous regression within the next 5‑10 years, frequently leaving fibrous fat residues. However, the specific mechanisms underlying the differentiation of hemangioma stem cells (HemSCs) into adipocytes are not clear. The purpose of the present study was to inv...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

circRNA ‑miRNA association for coronary heart disease.
circRNA‑miRNA association for coronary heart disease. Mol Med Rep. 2019 Jan 28;: Authors: Lin F, Zhao G, Chen Z, Wang X, Lv F, Zhang Y, Yang X, Liang W, Cai R, Li J, Li M, Zhang G Abstract Coronary heart disease (CHD) is a major cause of morbidity and mortality and an important public health problem globally, but the mechanism of CHD is still complex and unclear. The purpose of the current study was to explore the mechanism underlying CHD using high‑throughput technology. The study participants were patients with coronary angiography (CAG)‑proven severity of coronary artery stenosis. Patients wer...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Functional genetic variants of the GATA4 gene promoter in acute myocardial infarction.
Authors: Chen J, Wang S, Pang S, Cui Y, Yan B, Hawley RG Abstract Coronary artery disease (CAD), including acute myocardial infarction (AMI), is a common complex disease; however, the genetic causes remain largely unknown. Recent epidemiological investigations indicated that the incidence of CAD in patients with congenital heart diseases is markedly higher than that observed in healthy controls. It was therefore hypothesized that the dysregulated expression of cardiac developmental genes may be involved in CAD development. GATA binding protein 4 (GATA4) serves essential roles in heart developmen...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Expression and role of TNIP2 in multiple organ dysfunction syndrome following severe trauma.
In conclusion, the data indicated that TNIP2 is significantly decreased in MODS following severe trauma, and it plays a protective role in MODS development by inhibiting the inflammation response and oxidative stress by preventing NF‑κB activation. PMID: 30720079 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

BP ‑1‑102 exerts an antitumor effect on the AGS human gastric cancer cell line through modulating the STAT3 and MAPK signaling pathways.
BP‑1‑102 exerts an antitumor effect on the AGS human gastric cancer cell line through modulating the STAT3 and MAPK signaling pathways. Mol Med Rep. 2019 Jan 24;: Authors: Jiang X, Tang J, Wu M, Chen S, Xu Z, Wang H, Wang H, Yu X, Li Z, Teng L Abstract BP‑1‑102, a novel inhibitor of signal transducer and activator of transcription 3 (STAT3), exhibits significant antitumor effects in several malignancies in vitro and in vivo. However, its role in gastric cancer (GC) remains to be elucidated. In the present study, the effect and potential molecular mechanisms of BP‑102 in human GC cel...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Simultaneous detection of target CNVs and SNVs of thalassemia by multiplex PCR and next ‑generation sequencing.
Simultaneous detection of target CNVs and SNVs of thalassemia by multiplex PCR and next‑generation sequencing. Mol Med Rep. 2019 Jan 24;: Authors: Fan DM, Yang X, Huang LM, Ouyang GJ, Yang XX, Li M Abstract Thalassemia is caused by complex mechanisms, including copy number variants (CNVs) and single nucleotide variants (SNVs). The CNV types of α‑thalassemia are typically detected by gap‑polymerase chain reaction (PCR). The SNV types are detected by Sanger sequencing. In the present study, a novel method was developed that simultaneously detects CNVs and SNVs by multiplex PCR and next‑gene...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Knockdown of ARK5 expression suppresses invasion of ovarian cancer cells.
Authors: Wang S, Li S, Wang H, Li W, Gao Y, Wang X, Fang C, Zhang B, Sun X, Li R, Shi W, Chen M, Shi L Abstract The aim of the current study was to investigate the effects and the molecular mechanisms of ARK5 in ovarian cancer cell invasion. The plasmid pGCsilencerU6/GFP/Neo‑RNAi‑ARK5 and the control vector with a scramble sequence were transfected into SKOV3 cells to establish ARK5‑deficient SKOV3 cells (siARK5/SKOV3) and a control cell line (Scr/SKOV3), respectively. Reverse transcription‑polymerase chain reaction (RT‑PCR) and Western blot analysis were used to determine the mRNA and protein expression ...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Upregulation of ADAMTS ‑7 and downregulation of COMP are associated with spontaneous abortion.
Upregulation of ADAMTS‑7 and downregulation of COMP are associated with spontaneous abortion. Mol Med Rep. 2019 Jan 28;: Authors: Mu Y, Zhou DN, Yan NN, Ding JL, Yang J Abstract A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS‑7) has been revealed to serve an important role in inflammation‑associated diseases. However, the role of ADAMTS‑7 in spontaneous abortion (SA) remains unclear. In the present study, human and mouse decidual tissues were used to detect the expression of ADAMTS‑7 and cartilage oligomeric matrix protein (COMP) in mice with lipopolysaccharide (LPS)...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Construction of a cDNA library and preliminary analysis of the expressed sequence tags of the earthworm Eisenia  fetida (Savigny, 1826).
Construction of a cDNA library and preliminary analysis of the expressed sequence tags of the earthworm Eisenia fetida (Savigny, 1826). Mol Med Rep. 2019 Feb 05;: Authors: Liu C, Wang X, Tian JB, Ma PY, Meng FX, Zhang Q, Yu BF, Guo R, Liu ZZ, Wang HL, Xie J, Cheng NL, Wang JH, Niu B, Sun GQ Abstract Earthworms are useful indicator organisms of soil health and Eisenia fetida have been extensively used as test organisms in ecotoxicological studies. In order to gain insight into the gene expression profiles associated with physiological functions of earthworms, a full‑length enriched cDNA libr...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Investigating age ‑induced differentially expressed genes and potential molecular mechanisms in osteosarcoma based on integrated bioinformatics analysis.
Investigating age‑induced differentially expressed genes and potential molecular mechanisms in osteosarcoma based on integrated bioinformatics analysis. Mol Med Rep. 2019 Jan 30;: Authors: Wang JS, Duan MY, Zhong YS, Li XD, Du SX, Xie P, Zheng GZ, Han JM Abstract Osteosarcoma (OS) is the most common primary bone malignancy. It predominantly occurs in adolescents, but can develop at any age. The age at diagnosis is a prognostic factor of OS, but the molecular basis of this remains unknown. The current study aimed to identify age‑induced differentially expressed genes (DEGs) and potential molecular m...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

The anthelmintic agent oxfendazole inhibits cell growth in non ‑small cell lung cancer by suppressing c‑Src activation.
The anthelmintic agent oxfendazole inhibits cell growth in non‑small cell lung cancer by suppressing c‑Src activation. Mol Med Rep. 2019 Jan 28;: Authors: Xu D, Tian W, Jiang C, Huang Z, Zheng S Abstract The c‑Src protein family of tyrosine kinases are important in the tumorigenesis of many types of tumors, and may be a potential target for antitumor drug discovery. In the present study, immunoblotting was performed to analyze protein expression, CCK‑8 assay was carried out to assess cell viability and cell cycle was analyzed using a flow cytometer. The anthelmintic agent oxfendazole was observ...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Potential urine biomarkers for gestational hypertension and preeclampsia.
Authors: Guo HX, Zhu YB, Wu CP, Zhong M, Hu SW Abstract Differential proteomic technology was used to identify urine proteomic profile of gestational hypertension and preeclampsia. Urine samples were collected from 10 patients with gestational hypertension, 10 patients with mild preeclampsia, 10 patients with severe preeclampsia and 10 normal pregnancies and analyzed by 2‑D difference gel electrophoresis, then matrix assisted laser desorption ionization mass spectrometry was used to identify differential proteins. Subsequently, ELISA was used to verify the content variation of the identified p...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

MicroRNA ‑504 targets AEG‑1 and inhibits cell proliferation and invasion in retinoblastoma.
In conclusion, these results indicated a significant role of the miR‑504/AEG‑1 pathway in inhibiting the aggressiveness of RB, suggesting that this miRNA may be employed as a therapeutic target for the treatment of patients with this disease. PMID: 30720088 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Regulation of Fgf15 expression in the intestine by glucocorticoid receptor.
Authors: Jia K, Zhang D, Jia Q, Zhang QY Abstract Fibroblast growth factor 15 (FGF15) was previously identified to be highly expressed in the ileum and functions as an endocrine factor to regulate bile acid synthesis in the liver. FGF15 targets its receptor fibroblast growth factor receptor 4 in the liver and serves important roles in energy metabolism, including bile acid homeostasis, glucose metabolism and protein synthesis. The expression of FGF15 is known to be regulated by the transcription factor farnesoid X receptor (FXR). In the present study, reverse transcription‑quantitative polymerase chain react...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

JMJD2A facilitates growth and inhibits apoptosis of cervical cancer cells by downregulating tumor suppressor miR ‑491‑5p.
JMJD2A facilitates growth and inhibits apoptosis of cervical cancer cells by downregulating tumor suppressor miR‑491‑5p. Mol Med Rep. 2019 Jan 31;: Authors: Li Y, Wang Y, Xie Z, Hu H Abstract Cervical cancer remains the second most common malignancy for women worldwide. Jumonji domain containing 2A (JMJD2A), a member of the JmjC domain‑containing family of JMJD2 proteins, is capable of regulating cancer‑associated genes, including genes involved in the cell cycle, proliferation, apoptosis, invasion and metastasis. However, its role in human cervical cancer has yet to be elucidated. microRN...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice.
Authors: Peng L, Xie Z, Pei J, Wang B, Gao Y, Qu Y Abstract Recent studies have suggested that puerarin may impede osteoclastogenesis and facilitate bone regeneration, in addition to attenuating tissue inflammation. The present study investigated the therapeutic effects of puerarin on inflammatory responses and monocyte recruitment in in vitro and in vivo osteoarthritis (OA) models. Puerarin treatment increased the proliferation of OA chondrocytes, as determined by Cell Counting Kit‑8 assay. In addition, the present results suggested that puerarin suppressed the interleukin‑1β‑induced productio...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Aberrant expression of sperm ‑specific glycolytic enzymes are associated with poor sperm quality.
This study reveals a close association of glycolytic enzymes with sperm quality. The data may greatly contribute to the molecular evaluation of sperm quality and the diagnosis and treatment of asthenozoospermia. PMID: 30720094 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Comprehensive circular RNA expression profiles in a mouse model of nonalcoholic steatohepatitis.
Authors: Ou Q, Zhao Y, Zhou J, Wu X Abstract Recent studies have revealed that circular RNAs (circRNAs) are involved in the development of various liver diseases. However, the regulatory role of circRNAs in nonalcoholic steatohepatitis (NASH) has not been fully elucidated. In the present study, the circRNA profiles in a NASH mouse model were investigated, and their functions in NASH were predicted using bioinformatics analysis, with the aim of providing novel clues for delineating the mechanisms of action. A NASH mouse model was established by feeding mice with a methionine and choline‑deficient diet. The liver c...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Integrative analysis of cancer driver genes in prostate adenocarcinoma.
Authors: Zhao X, Lei Y, Li G, Cheng Y, Yang H, Xie L, Long H, Jiang R Abstract Large‑scale genomics studies have identified recurrently mutated genes in the ETS gene family, including fusions and copy number variations (CNVs), which are involved in the development of prostate adenocarcinoma (PRAD). However, the aetiology of PRAD remains to be fully elucidated. In the present study, 333 driver genes were identified using four computational tools: OncodriveFM, OncodriveCLUST, iCAGES and DrGaP. In addition, 32 driver pathways were identified using DrGaP. SPOP, TP53, SPTA1, AHNAK, HMCN1, ATM, FOXA1, CSMD3, LRP1B and ...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Preliminary study on the role of miR ‑148a and DNMT1 in the pathogenesis of acute myeloid leukemia.
Preliminary study on the role of miR‑148a and DNMT1 in the pathogenesis of acute myeloid leukemia. Mol Med Rep. 2019 Jan 30;: Authors: Wang XX, Zhang H, Li Y Abstract MicroRNA (miR)‑148a is differentially expressed in numerous malignant tumors and it was identified to regulate tumor growth, cell proliferation, apoptosis, angiogenesis and drug resistance via the regulation of the expression levels of its target genes. However, the biological function of miR‑148a in acute myeloid leukemia (AML) and its molecular mechanisms of action remain unclear. In the present study, the expression levels of miR...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

RNA sequencing discloses the genome ‑wide profile of long noncoding RNAs in dilated cardiomyopathy.
In conclusion, the present study revealed the genome‑wide profile of circulating lncRNAs in DCM by RNA sequencing, and explored the potential functions of these lncRNAs in DCM using bioinformatics analysis. These findings provide a theoretical foundation for future studies of lncRNAs in DCM. PMID: 30720098 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

10 ‑Hydroxycamptothecin induces apoptosis in human fibroblasts by regulating miRNA‑23b‑3p expression.
10‑Hydroxycamptothecin induces apoptosis in human fibroblasts by regulating miRNA‑23b‑3p expression. Mol Med Rep. 2019 Feb 01;: Authors: Zeng L, Sun Y, Li X, Wang J, Yan L Abstract 10‑Hydroxycamptothecin (HCPT) effectively controls epidural fibrosis, but the exact underlying mechanisms remain ambiguous. Abnormal microRNA (miR)‑23b‑3p expression has been detected in various types of fibrotic tissues that are present in different diseases. The aim of the present study was to elucidate the mechanisms through which HCPT induces fibroblast apoptosis. Reverse transcription‑quantitative polymera...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Comprehensive bioinformatics analysis of critical lncRNAs, mRNAs and miRNAs in non ‑alcoholic fatty liver disease.
Comprehensive bioinformatics analysis of critical lncRNAs, mRNAs and miRNAs in non‑alcoholic fatty liver disease. Mol Med Rep. 2019 Feb 05;: Authors: Wu H, Song X, Ling Y, Zhou J, Tao Z, Shen Y Abstract Non‑alcoholic fatty liver disease (NAFLD) is the most common fatty liver disease in developed countries, in which fat accumulation in the liver is induced by non‑alcoholic factors. The present study was conducted to identify NAFLD‑associated long non‑coding RNAs (lncRNAs), mRNAs and microRNAs (miRNAs). The microarray dataset GSE72756, which included 5 NAFLD liver tissues and 5 contro...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Differences in the distribution of CD20, CD3, CD34 and CD45RO in nasal mucosa and polyps from patients with chronic rhinosinusitis.
We examined surgically resected nasal polyps from 127 patients diagnosed with CRSwNP, who benefited from surgical procedures at the Department of Otorhinolaryngology of our institution. The polyps were analyzed at the Department of Pathology of our institution utilizing histopathological and immunohistochemical methods as follows: Firstly, the tissues were paraffin‑impregnated, sectioned and stained with hematoxylin and eosin. We then examined the expression of CD3, CD20, CD34 and CD45RO by immunohistochemistry with soluble labeled streptavidin biotin (LSAB)/horseradish peroxidase (HRP) complexes. We observed the followi...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Tetramethylpyrazine reduces inflammation in the livers of mice fed a high fat diet.
In conclusion, TMP, similar to simvastatin, exerted a notable hepatoprotective effect on mice fed a high fat diet with non‑alcoholic fatty liver disease, by inhibiting inflammatory factors and the p‑NF‑κB/ROS signaling pathway. PMID: 30720104 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Prediction and analysis of weighted genes in hepatocellular carcinoma using bioinformatics analysis.
In conclusion, 9 weighted genes, involved in the development and progression of HCC, were identified using bioinformatics and survival analyses. PMID: 30720105 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Implantation of engineered conduction tissue in the rat heart.
In conclusion, ECTs can survive and mechanically integrate with the allogeneic myocardium following transplantation into rat hearts. An atrioventricular accessory pathway similar to Kent bundles could be established between the atria and ventricles of rats following implantation. It is suggested that ECTs may be a potential substitution therapy for atrioventricular conduction block. PMID: 30720107 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Fluoxetine induces direct inhibitory effects on mesenchymal stem cell ‑derived osteoprogenitor cells independent of serotonin concentration.
Fluoxetine induces direct inhibitory effects on mesenchymal stem cell‑derived osteoprogenitor cells independent of serotonin concentration. Mol Med Rep. 2019 Feb 01;: Authors: Koura SM, Salama M, El-Hussiny M, Khalil MEA, Lotfy A, Hassan SA, Gad Elhak SA, Sobh MA Abstract Selective serotonin reuptake inhibitors are the most commonly prescribed antidepressants worldwide, which have been reported to exert potential detrimental effects on bone mineral density and increase the risk of developing fractures. The present study aimed to investigate the pathways underlying the negative effects of fluoxetine o...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

PGAM1 knockdown is associated with busulfan ‑induced hypospermatogenesis and spermatogenic cell apoptosis.
PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis. Mol Med Rep. 2019 Feb 04;: Authors: Zhao Y, Zhang S Abstract Phosphoglycerate mutase 1 (PGAM1) is reported to be involved in spermatogenic dysfunction. However, the association between PGAM1 and busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis remains unclear. The aim of the current study was to investigate the association between PGAM1 expression and busulfan‑induced hypospermatogenesis, and the effect of PGAM1 expression on spermatogenic cell apoptosis. PGAM1 expression...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

[Retracted] Pathogenetic mechanism of lipoid proteinosis caused by mutation of the extracellular matrix protein 1 gene.
Authors: Gao D, Ma X, Lian P, Zhou S, Chen J Abstract After the publication of the above article, the Editor of Molecular Medicine Reports received an accusation that this paper contained material taken from the PhD thesis of Dr Xiao Bai of the China Academy of Medical Science & Peking Union Medical College, whose thesis entitled "Study on mutations of ECM1 in lipoid proteinosis" was published online in 2015. After having enquired with the authors regarding this allegation, the first author and the corresponding author, Dr Dong Gao, admitted that she had infringed the rights of Dr Bai, ...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

MT1 ‑MMP promotes the proliferation and invasion of gastric carcinoma cells via regulating vimentin and E‑cadherin.
MT1‑MMP promotes the proliferation and invasion of gastric carcinoma cells via regulating vimentin and E‑cadherin. Mol Med Rep. 2019 Jan 31;: Authors: Li B, Lou G, Zhou J Abstract The present study aimed to explore the possible effects of membrane‑type 1 matrix metalloproteinase (MT1‑MMP) on gastric carcinoma cells proliferation and invasion. Immunohistochemistry analysis was conducted to measure MT1‑MMP expression level in 15 patients with gastric carcinoma. Subsequently, recombinant short hairpin RNA (shRNA) vectors targeting MT1‑MMP were constructed to silence the expression of...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

miR ‑155 mediates inflammatory injury of hippocampal neuronal cells via the activation of microglia.
In conclusion, the present results suggested that miR‑155 mediated the inflammatory injury in hippocampal neuronal cells by activating the microglial cells. The potential effects of miR‑155 on the activation of microglial cells suggest that miR‑155 may be an effective target for TRD therapies. PMID: 30720115 [PubMed - as supplied by publisher] (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

MicroRNA ‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma.
MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma. Mol Med Rep. 2019 Feb 05;: Authors: Guo L, Li B, Miao M, Yang J, Ji J Abstract Previous studies have demonstrated that numerous tumor‑specific microRNAs (miRNAs) are upregulated or downregulated in hepatocellular carcinoma (HCC), and that their dysregulation is implicated in HCC occurrence and development. Therefore, investigation of crucial miRNAs involved in HCC oncogenesis and progression may provide novel insights into the therapy of patients with this malignant tumor. In the present study, rever...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Identification of a pathogenic mutation in a Chinese pedigree with polycystic kidney disease.
Authors: Dong K, Miao H, Jia X, Wu J, Wu H, Sun J, Ji W, Su H, Xu L, Zhang X, Zhu S, Ji G, Guan R, Wang H, Bai J, Yu J, Sun W, Zhou X, Fu S Abstract Polycystic kidney disease (PKD) is a life‑threatening inherited disease with a morbidity of 1:500‑1,000 worldwide. Numerous progressively enlarging cysts are observed in the bilateral kidneys of patients with PKD, inducing structural damage and loss of kidney function. The present study analyzed one family with PKD. Whole exome sequencing of the proband was performed to detect the pathogenic gene present in the family. Candidate gene segments for lineal consanguini...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

NLRP3/IL1 β inflammasome associated with the aging bladder triggers bladder dysfunction in female rats.
NLRP3/IL1β inflammasome associated with the aging bladder triggers bladder dysfunction in female rats. Mol Med Rep. 2019 Jan 31;: Authors: Chen L, He PL, Yang J, Yang YF, Wang K, Amend B, Stenzl A, Zhang YM, Wang ZL, Xing SS, Luo X Abstract Bladder dysfunction is associated with fibrosis‑-mediated aging, but the corresponding mechanism remains to be elucidated. Activation of the NACHT, LRR and PYD domains‑containing protein 3 (NLRP3) inflammasome is related to chronic diseases associated with aging, including organ fibrosis. The present study aimed to explore the role of NLRP3/interleukin 1&be...
Source: Molecular Medicine Reports - February 7, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research