Molecular and pathophysiological relationship between obesity and chronic inflammation in the manifestation of metabolic dysfunctions and their inflammation ‑mediating treatment options (Review)
Mol Med Rep. 2024 Jun;29(6):95. doi: 10.3892/mmr.2024.13219. Epub 2024 Apr 12.ABSTRACTObesity reaches up to epidemic proportions globally and increases the risk for a wide spectrum of co‑morbidities, including type‑2 diabetes mellitus (T2DM), hypertension, dyslipidemia, cardiovascular diseases, non‑alcoholic fatty liver disease, kidney diseases, respiratory disorders, sleep apnea, musculoskeletal disorders and osteoarthritis, subfertility, psychosocial problems and certain types of cancers. The underlying inflammatory mechanisms interconnecting obesity with metabolic dysfunction are not completely understood. Increas...
Source: Molecular Medicine Reports - April 12, 2024 Category: Molecular Biology Authors: Fani-Niki Varra Michail Varras Viktoria-Konstantina Varra Panagiotis Theodosis-Nobelos Source Type: research
Octreotide attenuates intestinal barrier damage by maintaining basal autophagy in Caco2 cells
This article is based on a cell model of inflammation induced by lipopolysaccharide (LPS), aiming to explore the effects of OCT in protecting intestinal mucosal barrier function. A Cell Counting Kit‑8 assay was used to determine cell viability and evaluate the effectiveness of OCT. Gene silencing technology was used to reveal the mediated effect of somatostatin receptor 2 (SSTR2). The changes in intestinal permeability were detected through trans‑epithelial electrical resistance and fluorescein isothiocyanate‑dextran 4 experiments, and the alterations in tight junction proteins were detected using immunoblotting and ...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Xiaoli Liu Yan Zhou Yu Zhang Xigang Cui Donglin Yang Yuling Li Source Type: research
Induction of the human < em > CDC45 < /em > gene promoter activity by natural compound < em > trans < /em > ‑resveratrol
Mol Med Rep. 2024 Jun;29(6):92. doi: 10.3892/mmr.2024.13216. Epub 2024 Apr 5.ABSTRACTGGAA motifs in the human TP53 and HELB gene promoters play a part in responding to trans‑resveratrol (Rsv) in HeLa S3 cells. This sequence is also present in the 5'‑upstream region of the human CDC45 gene, which encodes a component of CMG DNA helicase protein complex. The cells were treated with Rsv (20 µM), then transcripts and the translated protein were analyzed by quantitative RT‑PCR and western blotting, respectively. The results showed that the CDC45 gene and protein expression levels were induced after the treatment. To exami...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Jun Arakawa Hikaru Kondoh Tokiyoshi Matsushita Yoko Ogino Masashi Asai Sei-Ichi Tanuma Fumiaki Uchiumi Source Type: research
Long non ‑coding RNA DANCR aggravates breast cancer through the miR‑34c/E2F1 feedback loop
Mol Med Rep. 2024 Jun;29(6):93. doi: 10.3892/mmr.2024.13217. Epub 2024 Apr 5.ABSTRACTEmerging scientific evidence has suggested that the long non‑coding (lnc)RNA differentiation antagonizing non‑protein coding RNA (DANCR) serves a significant role in human tumorigenesis and cancer progression; however, the precise mechanism of its function in breast cancer remains to be fully understood. Therefore, the objective of the present study was to manipulate DANCR expression in MCF7 and MDA‑MB‑231 cells using lentiviral vectors to knock down or overexpress DANCR. This manipulation, alongside the analysis of bioinformatics ...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Shuai Yan Lizhi Teng Juntong Du Liang Ji Peng Xu Wenxi Zhao Weiyang Tao Source Type: research
Research progress on oncoprotein hepatitis B X ‑interacting protein (Review)
In conclusion, the present review summarized the existing knowledge of HBXIP, established its carcinogenic mechanism and discussed future related research on HBXIP.PMID:38577934 | DOI:10.3892/mmr.2024.13213 (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Lei Cheng Lijuan Guo Teng Zou Yisong Yang Ran Tao Shuangping Liu Source Type: research
[Corrigendum] Long non ‑coding RNA NEAT1 promotes ovarian cancer cell invasion and migration by interacting with miR‑1321 and regulating tight junction protein 3 expression
Mol Med Rep. 2024 Jun;29(6):91. doi: 10.3892/mmr.2024.13215. Epub 2024 Apr 5.ABSTRACTSubsequently to the publication of the above article, an interested reader drew to the authors' attention that, for the cell invasion and migration assay images shown for the A2780 cell line in Figs. 1 and Fig. 3 on p. 3433 and 3435 respectively, the same data panel had apparently been selected to show the results of the si‑NEAT1 experiment in Fig. 1 and the si‑TJP3 experiment in Fig. 3. After having re‑examined their original data, the authors have realized that the image correctly shown for Fig. 1 was inadvertently copied across to...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Min Luo Lei Zhang Hongying Yang Kaili Luo Chen Qing Source Type: research
[Retracted] MicroRNA ‑25 protects nucleus pulposus cells against apoptosis via targeting SUMO2 in intervertebral disc degeneration
Mol Med Rep. 2024 Jun;29(6):88. doi: 10.3892/mmr.2024.13212. Epub 2024 Apr 5.ABSTRACTFollowing the publication of this paper, the authors requested that the paper be retracted, specifically on account of deficiencies that were identified both in the documentation of patient records and in written consent pertaining to the data presented in Fig. 1. After having considered the authors' request, the Editor of Molecular Medicine Reports has agreed that this paper should be retracted from the Journal. All the authors are in agreement with the decision to retract this paper. The Editor apologizes to the readership for any inconv...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Changbin Lei Jian Li Guang Tang Jiong Wang Source Type: research
Octreotide attenuates intestinal barrier damage by maintaining basal autophagy in Caco2 cells
This article is based on a cell model of inflammation induced by lipopolysaccharide (LPS), aiming to explore the effects of OCT in protecting intestinal mucosal barrier function. A Cell Counting Kit‑8 assay was used to determine cell viability and evaluate the effectiveness of OCT. Gene silencing technology was used to reveal the mediated effect of somatostatin receptor 2 (SSTR2). The changes in intestinal permeability were detected through trans‑epithelial electrical resistance and fluorescein isothiocyanate‑dextran 4 experiments, and the alterations in tight junction proteins were detected using immunoblotting and ...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Xiaoli Liu Yan Zhou Yu Zhang Xigang Cui Donglin Yang Yuling Li Source Type: research
Induction of the human < em > CDC45 < /em > gene promoter activity by natural compound < em > trans < /em > ‑resveratrol
Mol Med Rep. 2024 Jun;29(6):92. doi: 10.3892/mmr.2024.13216. Epub 2024 Apr 5.ABSTRACTGGAA motifs in the human TP53 and HELB gene promoters play a part in responding to trans‑resveratrol (Rsv) in HeLa S3 cells. This sequence is also present in the 5'‑upstream region of the human CDC45 gene, which encodes a component of CMG DNA helicase protein complex. The cells were treated with Rsv (20 µM), then transcripts and the translated protein were analyzed by quantitative RT‑PCR and western blotting, respectively. The results showed that the CDC45 gene and protein expression levels were induced after the treatment. To exami...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Jun Arakawa Hikaru Kondoh Tokiyoshi Matsushita Yoko Ogino Masashi Asai Sei-Ichi Tanuma Fumiaki Uchiumi Source Type: research
Long non ‑coding RNA DANCR aggravates breast cancer through the miR‑34c/E2F1 feedback loop
Mol Med Rep. 2024 Jun;29(6):93. doi: 10.3892/mmr.2024.13217. Epub 2024 Apr 5.ABSTRACTEmerging scientific evidence has suggested that the long non‑coding (lnc)RNA differentiation antagonizing non‑protein coding RNA (DANCR) serves a significant role in human tumorigenesis and cancer progression; however, the precise mechanism of its function in breast cancer remains to be fully understood. Therefore, the objective of the present study was to manipulate DANCR expression in MCF7 and MDA‑MB‑231 cells using lentiviral vectors to knock down or overexpress DANCR. This manipulation, alongside the analysis of bioinformatics ...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Shuai Yan Lizhi Teng Juntong Du Liang Ji Peng Xu Wenxi Zhao Weiyang Tao Source Type: research
Research progress on oncoprotein hepatitis B X ‑interacting protein (Review)
In conclusion, the present review summarized the existing knowledge of HBXIP, established its carcinogenic mechanism and discussed future related research on HBXIP.PMID:38577934 | DOI:10.3892/mmr.2024.13213 (Source: Molecular Medicine Reports)
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Lei Cheng Lijuan Guo Teng Zou Yisong Yang Ran Tao Shuangping Liu Source Type: research
[Corrigendum] Long non ‑coding RNA NEAT1 promotes ovarian cancer cell invasion and migration by interacting with miR‑1321 and regulating tight junction protein 3 expression
Mol Med Rep. 2024 Jun;29(6):91. doi: 10.3892/mmr.2024.13215. Epub 2024 Apr 5.ABSTRACTSubsequently to the publication of the above article, an interested reader drew to the authors' attention that, for the cell invasion and migration assay images shown for the A2780 cell line in Figs. 1 and Fig. 3 on p. 3433 and 3435 respectively, the same data panel had apparently been selected to show the results of the si‑NEAT1 experiment in Fig. 1 and the si‑TJP3 experiment in Fig. 3. After having re‑examined their original data, the authors have realized that the image correctly shown for Fig. 1 was inadvertently copied across to...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Min Luo Lei Zhang Hongying Yang Kaili Luo Chen Qing Source Type: research
[Retracted] MicroRNA ‑25 protects nucleus pulposus cells against apoptosis via targeting SUMO2 in intervertebral disc degeneration
Mol Med Rep. 2024 Jun;29(6):88. doi: 10.3892/mmr.2024.13212. Epub 2024 Apr 5.ABSTRACTFollowing the publication of this paper, the authors requested that the paper be retracted, specifically on account of deficiencies that were identified both in the documentation of patient records and in written consent pertaining to the data presented in Fig. 1. After having considered the authors' request, the Editor of Molecular Medicine Reports has agreed that this paper should be retracted from the Journal. All the authors are in agreement with the decision to retract this paper. The Editor apologizes to the readership for any inconv...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Changbin Lei Jian Li Guang Tang Jiong Wang Source Type: research
Octreotide attenuates intestinal barrier damage by maintaining basal autophagy in Caco2 cells
This article is based on a cell model of inflammation induced by lipopolysaccharide (LPS), aiming to explore the effects of OCT in protecting intestinal mucosal barrier function. A Cell Counting Kit‑8 assay was used to determine cell viability and evaluate the effectiveness of OCT. Gene silencing technology was used to reveal the mediated effect of somatostatin receptor 2 (SSTR2). The changes in intestinal permeability were detected through trans‑epithelial electrical resistance and fluorescein isothiocyanate‑dextran 4 experiments, and the alterations in tight junction proteins were detected using immunoblotting and ...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Xiaoli Liu Yan Zhou Yu Zhang Xigang Cui Donglin Yang Yuling Li Source Type: research
Induction of the human < em > CDC45 < /em > gene promoter activity by natural compound < em > trans < /em > ‑resveratrol
Mol Med Rep. 2024 Jun;29(6):92. doi: 10.3892/mmr.2024.13216. Epub 2024 Apr 5.ABSTRACTGGAA motifs in the human TP53 and HELB gene promoters play a part in responding to trans‑resveratrol (Rsv) in HeLa S3 cells. This sequence is also present in the 5'‑upstream region of the human CDC45 gene, which encodes a component of CMG DNA helicase protein complex. The cells were treated with Rsv (20 µM), then transcripts and the translated protein were analyzed by quantitative RT‑PCR and western blotting, respectively. The results showed that the CDC45 gene and protein expression levels were induced after the treatment. To exami...
Source: Molecular Medicine Reports - April 5, 2024 Category: Molecular Biology Authors: Jun Arakawa Hikaru Kondoh Tokiyoshi Matsushita Yoko Ogino Masashi Asai Sei-Ichi Tanuma Fumiaki Uchiumi Source Type: research
