Query: siRNA

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Total 14391 results found since Jan 2013.

Tocopherol suppresses 24(S)-hydroxycholesterol-induced cell death via inhibition of CaMKII phosphorylation.
Abstract Although 24(S)-hydroxycholesterol (24S-OHC) plays an important role to maintain homeostasis of cholesterol in the brain, it induces neuronal cell death at high concentrations. 24S-OHC-induced cell death was suppressed by γ-tocopherol (γ-Toc) but not by γ-tocotrienol (γ-Toc3) in a similar way to α-tocopherol (α-Toc) and α-tocotrienol (α-Toc3) in human neuroblastoma SH-SY5Y cells. Both γ-Toc and γ-Toc3 significantly inhibited cumene hydroperoxide-induced cell death, as previously shown in the case of α-Toc and α-Toc3. Lipid droplet-like structure formation induced by 24S-OHC was suppressed by ne...
Source: Biochimie - September 26, 2018 Category: Biochemistry Authors: Kimura Y, Asa M, Urano Y, Saito Y, Nishikawa K, Noguchi N Tags: Biochimie Source Type: research

Copper Transporter ATP7A Interacts with IQGAP1, a Rac1 Binding Scaffold Protein: Role in PDGF-induced VSMC Migration and Vascular Remodeling.
Abstract Vascular smooth muscle cell (VSMC) migration contributes to neointimal formation after vascular injury. We previously demonstrated that copper (Cu) transporter ATP7A is involved in PDGF-induced VSMC migration in Cu- and Rac1-dependent manner. Underlying mechanism is still unknown. Here we show that ATP7A interacts with IQGAP1, a Rac1- and receptor tyrosine kinase-binding scaffold proteins, which mediates PDGF-induced VSMC migration and vascular remodeling. In cultured rat aortic SMCs, PDGF stimulation rapidly promoted ATP7A association with IQGAP1 and Rac1, and their translocation to lipid rafts and leadi...
Source: Am J Physiol Cell Ph... - September 26, 2018 Category: Cytology Authors: Ashino T, Kohno T, Sudhahar V, Ash D, Ushio-Fukai M, Fukai T Tags: Am J Physiol Cell Physiol Source Type: research

Augmenter of liver regeneration promotes mitochondrial biogenesis in renal ischemia –reperfusion injury
AbstractMitochondria are the center of energy metabolism in the cell and the preferential target of various toxicants and ischemic injury. Renal ischemia –reperfusion (I/R) injury triggers proximal tubule injury and the mitochondria are believed to be the primary subcellular target of I/R injury. The promotion of mitochondrial biogenesis (MB) is critical for the prevention I/R injury. The results of our previous study showed that augmenter of liver regeneration (ALR) has anti-apoptotic and anti-oxidant functions. However, the modulatory mechanism of ALR remains unclear and warrants further investigation. To gain further ...
Source: Apoptosis - September 26, 2018 Category: Molecular Biology Source Type: research

Camptothecin enhances c-Myc-mediated endoplasmic reticulum stress and leads to autophagy by activating Ca2+-mediated AMPK.
In this study, we found that CPT enhanced c-Myc expression and that the transient knockdown of c-Myc abrogated reactive oxygen species (ROS) generation, which resulted in the accumulation of ER stress-regulating proteins, such as PERK, eIF2α, ATF4, and CHOP. Moreover, the transfection of eIF2α-targeted siRNA attenuated CPT-induced autophagy and decreased the levels of Beclin-1 and Atg7, which indicated that CPT upregulated ER stress-mediated autophagy. In addition, CPT phosphorylated AMPK in response to intracellular Ca2+ release. Ca2+ chelators, ethylene glycol tetraacetic acid and a CaMKII inhibitor, K252a, decreased C...
Source: Food and Chemical Toxicology - September 25, 2018 Category: Food Science Authors: Jayasooriya RGPT, Dilshara MG, Karunarathne WAHM, Molagoda IMN, Choi YH, Kim GY Tags: Food Chem Toxicol Source Type: research

IFN-{gamma}-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD
Interferon-gamma (IFN-) is a pleiotropic cytokine that exerts important functions in inflammation, infectious diseases, and cancer. The large GTPase human guanylate-binding protein 1 (GBP-1) is among the most strongly IFN--induced cellular proteins. Previously, it has been shown that GBP-1 mediates manifold cellular responses to IFN- including the inhibition of proliferation, spreading, migration, and invasion and through this exerts anti-tumorigenic activity. However, the mechanisms of GBP-1 anti-tumorigenic activities remain poorly understood. Here, we elucidated the molecular mechanism of the human GBP-1-mediated suppre...
Source: Biochemical Journal - September 25, 2018 Category: Biochemistry Authors: Unterer, B., Wiesmann, V., Gunasekaran, M., Sticht, H., Tenkerian, C., Behrens, J., Leone, M., Engel, F. B., Britzen-Laurent, N., Naschberger, E., Wittenberg, T., Stürzl, M. Tags: Research Articles Source Type: research

Hypoxia Induces the Acquisition of Cancer Stem-like Phenotype Via Upregulation and Activation of Signal Transducer and Activator of Transcription-3 (STAT3) in MDA-MB-231, a Triple Negative Breast Cancer Cell Line
In conclusion, our data have provided the proof-of-concept that STAT3 is a critical mediator in promoting the hypoxia-induced acquisition of cancer stemness in TNBC. Targeting STAT3 in TNBC may be useful in overcoming chemoresistance and decreasing the risk of disease relapse.
Source: Cancer Microenvironment - September 25, 2018 Category: Cancer & Oncology Source Type: research

Optimization of siRNA delivery to target sites: issues and future directions
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Source: Expert Opinion on Drug Delivery - September 25, 2018 Category: Drugs & Pharmacology Authors: Ikramy A. Khalil Yuma Yamada Hideyoshi Harashima Source Type: research

Hypoxia Induces the Acquisition of Cancer Stem-like Phenotype Via Upregulation and Activation of Signal Transducer and Activator of Transcription-3 (STAT3) in MDA-MB-231, a Triple Negative Breast Cancer Cell Line
In conclusion, our data have provided the proof-of-concept that STAT3 is a critical mediator in promoting the hypoxia-induced acquisition of cancer stemness in TNBC. Targeting STAT3 in TNBC may be useful in overcoming chemoresistance and decreasing the risk of disease relapse.
Source: Cancer Microenvironment - September 25, 2018 Category: Cancer & Oncology Source Type: research

Contribution of the clock gene DEC2 to VEGF mRNA upregulation by modulation of HIF1 α protein levels in hypoxic MIO-M1 cells, a human cell line of retinal glial (Müller) cells
ConclusionDEC2 could upregulate retinalVEGF gene expression through modulation of HIF1 α levels under hypoxic conditions.
Source: Japanese Journal of Ophthalmology - September 24, 2018 Category: Opthalmology Source Type: research

NEK4 kinase regulates EMT to promote lung cancer metastasis.
Abstract Epithelial-to-mesenchymal transition (EMT) is a dynamic transitional state from the epithelial to mesenchymal phenotypes. Numerous studies have suggested that EMT and its intermediate states play important roles in tumor invasion and metastasis. To identify novel regulatory molecules of EMT, we screened a siRNA library targeting human 720 kinases in A549 lung adenocarcinoma cells harboring E-cadherin promoter-luciferase reporter vectors. NIMA-related kinase-4 (NEK4) was identified and characterized as a positive regulator of EMT in the screening. Suppression of NEK4 resulted in the inhibition of cell migr...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Ding NH, Zhang L, Xiao Z, Rong ZX, Li Z, He J, Chen L, Ou DM, Liao WH, Sun LQ Tags: J Cell Mol Med Source Type: research

Meridianin C inhibits the growth of YD-10B human tongue cancer cells through macropinocytosis and the down-regulation of Dickkopf-related protein-3.
Abstract Meridianin C is a marine natural product known for its anti-cancer activity. At present, the anti-tumour effects of meridianin C on oral squamous cell carcinoma are unknown. Here, we investigated the effect of meridianin C on the proliferation of four different human tongue cancer cells, YD-8, YD-10B, YD-38 and HSC-3. Among the cells tested, meridianin C most strongly reduced the growth of YD-10B cells; the most aggressive and tumorigenic of the cell lines tested. Strikingly, meridianin C induced a significant accumulation of macropinosomes in the YD-10B cells; confirmed by the microscopic and TEM analysi...
Source: J Cell Mol Med - September 24, 2018 Category: Molecular Biology Authors: Park NS, Park YK, Ramalingam M, Yadav AK, Cho HR, Hong VS, More KN, Bae JH, Bishop-Bailey D, Kano J, Noguchi M, Jang IS, Lee KB, Lee J, Choi JS, Jang BC Tags: J Cell Mol Med Source Type: research

Growth hormone and Insulin-like growth factor-I (IGF-I) modulate the expression of L-type amino acid transporters in the muscles of spontaneous dwarf rats and L6 and C2C12 myocytes.
CONCLUSIONS: IGF-I increased LAT1 mRNA level in myocytes. However, the role of LAT1 in IGF-I-induced mTORC1 activation and cell functions remains unclear. PMID: 30273774 [PubMed - as supplied by publisher]
Source: Growth Hormone and IGF Research - September 23, 2018 Category: Endocrinology Authors: Sawa R, Nishida H, Yamamoto Y, Wake I, Kai N, Kikkawa U, Okimura Y Tags: Growth Horm IGF Res Source Type: research

Inactivation of PRIM1 Function Sensitizes Cancer Cells to ATR and CHK1 Inhibitors.
Abstract The phosphoinositide 3-kinase-related kinase ATR is a central regulator of the DNA damage response. Its chemical inhibition eliminates subsets of cancer cells in various tumor types. This effect is caused at least partly by the synthetically lethal relationship between ATR and certain DNA repair genes. In a previous screen using an siRNA library against DNA repair genes, we identified PRIM1, a part of the polymerase α-primase complex, as acting synthetically lethal with ATR. Applying a genetic ATR knock-in model of colorectal cancer cells, we confirmed that PRIM1 depletion inhibited proliferation of ATR-...
Source: Neoplasia - September 23, 2018 Category: Cancer & Oncology Authors: Job A, Schmitt LM, von Wenserski L, Lankat-Buttgereit B, Gress TM, Buchholz M, Gallmeier E Tags: Neoplasia Source Type: research

miR-126 Suppresses Invasion and Migration of Malignant Glioma by Targeting Mature T Cell Proliferation 1 (MTCP1).
CONCLUSIONS miR-126 inhibits the migration of malignant glioma cells by inhibiting MTCP1. PMID: 30233082 [PubMed - in process]
Source: Medical Science Monitor - September 22, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Dependence of HSP27 cellular level on protein kinase CK2 discloses novel therapeutic strategies
ConclusionBeing HSP27 involved in several pathological conditions, including protein conformational diseases (i.e Cystic Fibrosis) and cancer, the need of drugs to modulate its activity is growing and CK2-targeting could represent a new strategy to reduce cellular HSP27 level.General significanceThis study identifies CK2 as a molecular target to control HSP27 cellular expression.
Source: Biochimica et Biophysica Acta (BBA) General Subjects - September 22, 2018 Category: Biochemistry Source Type: research

S100P and Ezrin promote trans-endothelial migration of triple negative breast cancer cells
ConclusionsFrom our data we conclude that S100P, Ezrin and EzrinThr-567 are involved in the trans-endothelial migration of TNBC cells and that they may serve as potential targets in TNBC patients.
Source: Cellular Oncology - September 22, 2018 Category: Cancer & Oncology Source Type: research

Exendin-4, a glucagon-like peptide-1 receptor agonist, reduces hepatic steatosis and endoplasmic reticulum stress by inducing nuclear factor erythroid-derived 2-related factor 2 nuclear translocation.
Abstract Activation of endoplasmic reticulum (ER) stress is involved in the development of nonalcoholic fatty liver disease. Glucagon-like peptide-1 (GLP-1) has been reported to reduce hepatic steatosis, but the underlying mechanism has not been fully elucidated. Here, we investigated whether exendin-4 (EX-4), a GLP-1 receptor analogue, improves hepatic steatosis through ER stress reduction. Furthermore, we explored which ER stress pathway is involved in this process, with a focus on the protein kinase RNA-like ER kinase (PERK)-nuclear factor erythroid-derived 2-related factor 2 (Nrf2) pathway. EX-4 treatment redu...
Source: Toxicology and Applied Pharmacology - September 22, 2018 Category: Toxicology Authors: Yoo J, Cho IJ, Jeong IK, Ahn KJ, Chung HY, Hwang YC Tags: Toxicol Appl Pharmacol Source Type: research

Transcription factor 7-like 2 controls matrix degradation through nuclear factor κB signaling and is repressed by microRNA-155 in nucleus pulposus cells
Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Jianchao Sun, Junmin Hong, Siguo Sun, Xiaofei Wang, Yan Peng, Jie Zhou, Yingjie Huang, Shuangxing Li, Weijian Chen, Chunhai Li, Kang Xu, Wei YeAbstractAimTCF7L2, a key transcription factor in the canonical Wnt pathway, plays a vital role in the matrix degradation of chondrocytes. However, it is unknown whether TCF7L2 is important in the matrix metabolism of inner gel-like nucleus pulposus (NP) cells; thus, the aim of this study was to explore the effect and mechanism of TCF7L2 in this process.MethodsWestern blotting and immunofluores...
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Silencing Bmi1 expression suppresses cancer stemness and enhances chemosensitivity in endometrial cancer cells
ConclusionBmi1 silencing suppresses cancer stemness in HEC1A and Ishikawa cells. Concurrent treatment with Bmi1 siRNA and cisplatin resulted in additive anticancer effect with a cell line-specific pattern, which was higher than that shown by cisplatin treatment alone.Graphical abstract
Source: Biomedicine and Pharmacotherapy - September 21, 2018 Category: Drugs & Pharmacology Source Type: research

Downregulated caveolin-1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro.
In conclusion, the downregulated Cav-1 expression served a key role in NO production in the in vitro model of CAS induced by ACh stimulation of LPS-damaged HUVECs. PMID: 30233709 [PubMed]
Source: Experimental and Therapeutic Medicine - September 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Thymosin β4 promotes glucose-impaired endothelial progenitor cell function via Akt/endothelial nitric oxide synthesis signaling pathway.
Authors: Qiu F, Song J, Bi X, Wang M, Zhao Y, Fu G Abstract Circulating endothelial progenitor cells (EPCs) are a subtype of hematopoietic stem cells, which can differentiate into endothelial cells and restore endothelial function. However, high glucose decreases the number and impairs the function of EPCs. A previous study showed that thymosin β4 (Tβ4), a pleiotropic peptide beneficial for multiple functions of various types of cells, could promote EPC migration and dose-dependently upregulate the phosphorylation of Akt and endothelial nitric oxide synthesis signaling (eNOS). In present study, the hypothesis tha...
Source: Experimental and Therapeutic Medicine - September 21, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Desmoglein-2 overexpression predicts poor prognosis in hepatocellular carcinoma patients.
CONCLUSIONS: Taken together, our results suggest that increased Dsg2 expression was associated with tumor progression in HCC and may function as a promising biomarker for unfavorable prognosis of HCC. PMID: 30229819 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - September 21, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Using Two-Fluid Nozzle for Spray Freeze Drying to Produce Porous Powder Formulation of Naked siRNA for Inhalation
In this study, we reported the use of two-fluid nozzle to produce spray freeze dried powder of small interfering RNA (siRNA). The effect of atomization gas flow rate and liquid feed rate were inspected initially using herring sperm DNA (hsDNA) as nucleic acid model. The atomization gas flow rate was found to have a major impact on the aerosol properties. The higher the atomization gas flow rate, the smaller the particle size, the higher the fine particle fraction (FPF). In contrast, the liquid feed rate had very minor effect. Subsequently, spray freeze dried siRNA powder was produced at various atomization gas flow rates. ...
Source: International Journal of Pharmaceutics - September 20, 2018 Category: Drugs & Pharmacology Source Type: research

Additive polyplexes to undertake siRNA therapy against CDC20 and survivin in breast cancer cells.
Abstract Small interfering RNA (siRNA) delivered to silence overexpressed genes associated with malignancies is a promising targeted therapy to decrease the uncontrolled growth of malignant cells. To create potent delivery agents for siRNA, here we formulated additive polyplexes of siRNA using linoleic acid-substituted polyethylenimine and additive polymers (hyaluronic acid, polyacrylic acid, dextran sulfate and methyl cellulose), and characterized their physicochemical properties and effectiveness. Incorporating polyanionic polymer along with anionic siRNA in polyplexes was found to decrease the ζ-potential of p...
Source: Biomacromolecules - September 17, 2018 Category: Biochemistry Authors: Parmar MB, K C RB, Löbenberg R, Uludag H Tags: Biomacromolecules Source Type: research

GSE116188 Transcriptional profiling of WM1976 cells with siRNA knockdown of the long non-coding RNA SLNCR
Contributors : Karyn Schmidt ; Carl NovinaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA-Seq was used to profile transcriptional changes induced by siRNA knockdown of the long non-coding RNA SLNCR (all isoforms).
Source: GEO: Gene Expression Omnibus - September 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Methotrexate induced cell death mechanisms in MCF-7 adenocarcinoma breast cancer cells: Enhanced cytotoxicity following dff45-siRNA pre-treatment
Publication date: Available online 30 August 2018Source: SynergyAuthor(s): Fatemeh Kiani, Negin Rasouli, Tahereh Kashkoolinejad, Shahrokh Safarian, Seyed Jalal Zargar, Nader SheibaniAbstractThere are many efforts to diminish risks associated with the use of cancer chemotherapeutic agents through design and utilization of new strategies including gene therapy. We previously showed knockdown of dff45 or overexpression of dff40, the important apoptosis regulators, resulted in enhanced sensitization of cancer cells to chemotherapeutic agents. Here we show that pre-treatment with dff45-siRNA additively increased the overall cyt...
Source: Synergy - August 31, 2018 Category: Molecular Biology Source Type: research

Lipid-based siRNA Nanodelivery Systems: a Learning Process for Improving Transfer from Concepts to Clinical Applications.
CONCLUSION: Formulation design should be increasingly addressed with industrial criteria; it should be based on quality by design and on the estimation of critical attributes that affect performance, and supported by a range of characterization techniques and appropriate analytical methods. PMID: 30160217 [PubMed - as supplied by publisher]
Source: Current Clinical Pharmacology - August 29, 2018 Category: Drugs & Pharmacology Authors: Perez SE, Carlucci AM Tags: Curr Clin Pharmacol Source Type: research

Dendrimer as a new potential carrier for topical delivery of siRNA: a comparative study of dendriplex vs. lipoplex for delivery of TNF-α siRNA
Publication date: Available online 27 August 2018Source: International Journal of PharmaceuticsAuthor(s): Palpandi Pandi, Anjali Jain, Nagavendra Kommineni, Maksim Ionov, Maria Bryszewska, Wahid KhanAbstractTopical delivery of siRNA is challenging task due to complex barrier property of stratum corneum and cationic lipid based carriers have been widely explored for this purpose due to improved permeation through skin. For gene delivery application, dendrimers are considered as efficient carrier due to their cationic nature and well-defined surface groups. However, they are not well explored for topical delivery. This work ...
Source: International Journal of Pharmaceutics - August 28, 2018 Category: Drugs & Pharmacology Source Type: research

Cationic Hyperbranched Polymers with Biocompatible Shells for siRNA Delivery.
Abstract Cationic hyperbranched polymers (HBP) were prepared by self-condensing vinyl polymerization of an atom transfer radical polymerization (ATRP) inimer containing a quaternary ammonium group. Two types of biocompatible shells, poly(oligoethylene glycol) methacrylate (polyOEGMA) and poly(2-(methylsulfinyl) ethyl methacrylate) (polyDMSO), were grafted respectively from HBP core to form core-shell structures with low molecular weight dispersity and high biocompatibility, polyOEGMA-HBP and polyDMSO-HBP. Both of the structures showed low cytotoxicity and good siRNA complexing ability. The efficacy of gene silenci...
Source: Biomacromolecules - August 27, 2018 Category: Biochemistry Authors: Li S, Omi M, Cartieri F, Konkolewicz D, Mao G, Gao H, Averick SE, Mishina Y, Matyjaszewski K Tags: Biomacromolecules Source Type: research

The cutting-edge technologies of siRNA delivery and their application in clinical trials.
Abstract siRNA therapeutics allows precise regulation of disease specific gene expression to treat various diseases. Although gene silencing approaches using siRNA therapeutics shows some promising results in the treatment of gene-related diseases, the practical applications has been limited by problems such as inefficient in vivo delivery to target cells and nonspecific immune responses after systemic or local administration. To overcome these issues, various in vivo delivery platforms have been introduced. Here we provide an overview for three different platform technologies for the in vivo delivery of therapeut...
Source: Archives of Pharmacal Research - August 22, 2018 Category: Drugs & Pharmacology Authors: Lee K, Jang B, Lee YR, Suh EY, Yoo JS, Lee MJ, Lee JY, Lee H Tags: Arch Pharm Res Source Type: research

Comparison of exosome-mimicking liposomes with conventional liposomes for intracellular delivery of siRNA
This study may encourage further pursuit of more exosome-mimicking delivery vehicles with higher efficiency and biocompatibility.Graphical abstract
Source: International Journal of Pharmaceutics - August 21, 2018 Category: Drugs & Pharmacology Source Type: research

Rscreenorm: normalization of CRISPR and siRNA screen data for more reproducible hit selection
Reproducibility of hits from independent CRISPR or siRNA screens is poor. This is partly due to data normalization primarily addressing technical variability within independent screens, and not the technical d...
Source: BMC Bioinformatics - August 20, 2018 Category: Bioinformatics Authors: Costa Bachas, Jasmina Hodzic, Johannes C. van der Mijn, Chantal Stoepker, Henk M. W. Verheul, Rob M. F. Wolthuis, Emanuela Felley-Bosco, Wessel N. van Wieringen, Victor W. van Beusechem, Ruud H. Brakenhoff and Ren ée X. de Menezes Tags: Methodology article Source Type: research

Molecules, Vol. 23, Pages 2028: Evaluation of Amino-Functional Polyester Dendrimers Based on Bis-MPA as Nonviral Vectors for siRNA Delivery
Malkoch Herein, we present the first evaluation of cationic dendrimers based on 2,2-bis(methylol)propionic acid (bis-MPA) as nonviral vectors for transfection of short interfering RNA (siRNA) in cell cultures. The study encompassed dendrimers of generation one to four (G1–G4), modified to bear 6–48 amino end-groups, where the G2–G4 proved to be capable of siRNA complexation and protection against RNase-mediated degradation. The dendrimers were nontoxic to astrocytes, glioma (C6), and glioblastoma (U87), while G3 and G4 exhibited concentration dependent toxicity towards primary ...
Source: Molecules - August 14, 2018 Category: Chemistry Authors: Patrik Stenstr öm Dario Manzanares Yuning Zhang Valentin Ce ña Michael Malkoch Tags: Article Source Type: research

Sequestration of a Transposon-Derived siRNA by a Target Mimic Imprinted Gene Induces Postzygotic Reproductive Isolation in Arabidopsis.
Abstract Genomic imprinting is an epigenetic phenomenon occurring in mammals and flowering plants, causing genes to be expressed depending on their parent of origin. In plants, genomic imprinting is mainly confined to the endosperm, a nutritive tissue supporting embryo growth, similar to the placenta in mammals. Here, we show that the paternally expressed imprinted gene PEG2 transcript sequesters the transposable element (TE)-derived small interfering RNA (siRNA) siRNA854 in the endosperm. siRNA854 is present in the vegetative cell of pollen and transferred to the central cell of the female gametophyte after fert...
Source: Developmental Cell - August 13, 2018 Category: Cytology Authors: Wang G, Jiang H, Del Toro de León G, Martinez G, Köhler C Tags: Dev Cell Source Type: research

Bioreducible Poly(Amino Ethers) Based mTOR siRNA Delivery for Lung Cancer
ConclusionsThe results demonstrate that the mPAE polymer based NPs show strong potential for siRNA delivery to lung cancer cells. It is anticipated that future modification can help improve the efficacy of nucleic acid delivery, leading to higher inhibition of lung cancer growthin vitro andin vivo.
Source: Pharmaceutical Research - August 13, 2018 Category: Drugs & Pharmacology Source Type: research

SiRNA silencing of VEGF, IGFs, and their receptors in human retinal microvascular endothelial cells.
Authors: Nicolau Y, Bany-Mohammed F, Cai CL, Aranda JV, Beharry KD Abstract Vascular endothelial growth factor (VEGF) is a potent mitogen that regulates proliferation, migration, and tube formation of endothelial cells (EC). VEGF has recently become a target for severe retinopathy of prematurity (ROP) therapy. We tested the hypothesis that a specific VEGF isoform and/or receptor acts synergistically with insulin-like growth factor (IGF)-I to alter normal retinal microvascular EC angiogenesis and RNA interference can be used to reverse VEGF effects. We used small interfering RNA (SiRNA) transfection to target VEGF i...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

High-degree and broad-spectrum resistance mediated by a combination of NIb siRNA and miRNA suppresses replication of necrotic and common strains of potato virus Y
In this study, we designed specific siRNAs and miRNAs to target two regions of the PVYO replicase gene (NIb). Eight plant expression vectors containing one or two sRNAs were constructed. Luciferase activity assays showed that the designed sRNAs successfully cleaved theNIb gene of PVYO and PVYN, and the vector carrying a combined siRNA- and miRNA-based short hairpin RNA (shRNA) demonstrated the strongest inhibitory effect. These effects were confirmed through the acquisition of PVYO and PVYN resistance in transgenic sRNA-expressingNicotiana tabacum plants. This phenomenon could be related to a plant defense mechanism in whi...
Source: Archives of Virology - August 10, 2018 Category: Virology Source Type: research

An enhanced RRM2 siRNA delivery to rheumatoid arthritis fibroblast-like synoviocytes through a liposome ‑protamine-DNA-siRNA complex with cell permeable peptides.
An enhanced RRM2 siRNA delivery to rheumatoid arthritis fibroblast-like synoviocytes through a liposome‑protamine-DNA-siRNA complex with cell permeable peptides. Int J Mol Med. 2018 Aug 08;: Authors: Wang X, Wang X, Sun J, Fu S Abstract Rheumatoid arthritis (RA) is considered to be a systemic autoimmune disease that induces systemic complications and progressive disability. It affects a large number of people. RA fibroblast‑like synoviocytes (RA‑FLS) promote the progression of RA through the secretion of proinflammatory cytokines and increasing invasiveness into the extracellular matrix. Therefo...
Source: International Journal of Molecular Medicine - August 8, 2018 Category: Molecular Biology Authors: Wang X, Wang X, Sun J, Fu S Tags: Int J Mol Med Source Type: research

Current non-viral siRNA delivery systems as a promissing treatment of skin diseases.
CONCLUSION: The treatment of skin diseases based on topical delivery of siRNA, which act by inhibiting the expression of target transcripts, offers many potential therapeutic advantages for suppressing genes into the skin. PMID: 30084329 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - August 7, 2018 Category: Drugs & Pharmacology Authors: Rosa J, Suzuki I, Kravicz M, Caron A, Pupo AV, Praca FG, Bentley MVLB Tags: Curr Pharm Des Source Type: research

A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle
ConclusionsThis study provides a minable data set for every human gene regarding its role in EBOV genome replication and transcription, shows that an FDA-approved drug targeting one of the identified pathways is highly efficacious in vitro, and demonstrates the power of life cycle modelling systems for conducting genome-wide host factor screens for BSL4 viruses.
Source: Genome Medicine - August 7, 2018 Category: Genetics & Stem Cells Source Type: research

Gene Silencing using siRNA for Preventing Liver Ischaemia-Reperfusion Injury.
CONCLUSION: siRNA therapeutic potential to preclude liver IRI can be improved by a better knowledge of what molecules to target and by using more efficient delivery strategies. PMID: 30084326 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - August 7, 2018 Category: Drugs & Pharmacology Authors: Marinho HS, Marcelino P, Soares H, Corvo ML Tags: Curr Pharm Des Source Type: research

Nanostructured Hyaluronic acid-based materials for the delivery of siRNA.
CONCLUSION: To overcome the problems associated with these emerging genetic tools, investigators have employed glycosaminoglycan HA-based biopolymers. This biopolymer offers a variety of properties such as biodegradability, biocompatibility, aqueous solubility, viscoelasticity, and non-immunogenicity. PMID: 30084325 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - August 7, 2018 Category: Drugs & Pharmacology Authors: Shah K, Chawla S, Agrawal A, Reddy G, Maheshwari R, Kalia K, Tekade RK Tags: Curr Pharm Des Source Type: research

TARGETED LIPOSOMES FOR siRNA DELIVERY TO CANCER.
CONCLUSION: siRNA delivery mediated by functionalized liposomes is promising, but clinical trials need to be conducted. PMID: 30084323 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - August 7, 2018 Category: Drugs & Pharmacology Authors: Eloy JO, Petrilli R, Raspantini GL, Lee RJ Tags: Curr Pharm Des Source Type: research

A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle
ConclusionsThis study provides a minable data set for every human gene regarding its role in EBOV genome replication and transcription, shows that an FDA-approved drug targeting one of the identified pathways is highly efficacious in vitro, and demonstrates the power of life cycle modelling systems for conducting genome-wide host factor screens for BSL4 viruses.
Source: Genome Medicine - August 7, 2018 Category: Genetics & Stem Cells Source Type: research

Reversible Covalent-Crosslinked Polycations with Enhanced Stability and ATP-Responsive Behavior for Improved siRNA Delivery.
In this study, we proposed a new strategy of reversible-covalent crosslinking to enhance stability in circulation and enable stimuli-disassembly of polyplexes in tumor cells. Here, 25k polyethyleneimine (PEI) was chosen as model polycations for verifying the hypothesis. HA-PEI conjugation was formed by the crosslinking of adenosine triphosphate grafted HA (HA-ATP) with phenylboronic acid grafted PEI (PEI-PBA), via the chemical reaction between PBA and ATP. Compared with non-covalent polyplex by electrostatic interaction (HA/PEI), HA-PEI exhibited much better colloidal stability and serum stability. The covered HA-ATP layer...
Source: Biomacromolecules - August 6, 2018 Category: Biochemistry Authors: Zhou Z, Zhang M, Liu Y, Li C, Zhang Q, Oupicky D, Sun M Tags: Biomacromolecules Source Type: research

Development of an Alcohol Dilution-Lyophilization Method for Preparing Lipid Nanoparticles Containing Encapsulated siRNA.
In this study, we report on the development of an "alcohol dilution-lyophilization method" for preparing siRNA-encapsulating LNPs. This method involves the use of a freeze-drying (lyophilization) method to remove the residual alcohol and to simultaneously concentrate the preparation. At first, the compositions of cryoprotectants and polyethylene glycol (PEG)-lipids that were used were optimized from the point of view of particle stabilization. A combination of sucrose and 1-(monomethoxy polyethyleneglycol5000)-2,3-dimyristoylglycerol (DMG-PEG5000) was found to have the most efficient cryoprotective activity for the LNPs. T...
Source: Biological and Pharmaceutical Bulletin - August 3, 2018 Category: Drugs & Pharmacology Authors: Shirane D, Tanaka H, Nakai Y, Yoshioka H, Akita H Tags: Biol Pharm Bull Source Type: research

GSE110567 RNA-seq analysis of equine tenocytes exposed to siRNA targeting scleraxis (Scx) to identify novel targets of Scx-mediated mechanotransduction
Contributors : Anne E Nichols ; Robert E Settlage ; Stephen R Werre ; Linda A DahlgrenSeries Type : Expression profiling by high throughput sequencingOrganism : Equus caballusEquine tenocytes were exposed to an siRNA targeting the transcription factor scleraxis (Scx) or a scramble control and maintained under cyclic strain. The resulting transcriptomes were then used for RNA-seq analysis. The goal of this study was to identify novel ways in which Scx facilitates mechanotransduction in tenocytes in order to better understand tenocyte biology.
Source: GEO: Gene Expression Omnibus - August 2, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Equus caballus Source Type: research