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Naringenin Upregulates AMPK-Mediated Autophagy to Rescue Neuronal Cells From β-Amyloid (1–42) Evoked Neurotoxicity
AbstractDeposition of an amyloid- β peptide is one of the first events in the pathophysiology of Alzheimer’s disease (AD) and is clinically characterized by Aβ plaques, tau tangles, and behavioral impairments that lead to neuronal death. A substantial number of studies encourage targeting the skewness in the production and degra dation of amyloid-β could be among the promising therapies in the disease. Neuronal autophagy has emerged for an essential role in the degradation of such toxic aggregate-prone proteins in various neurodegenerative diseases. We profiled a small library of common dietary compounds and identifie...
Source: Molecular Neurobiology - June 16, 2020 Category: Neurology Source Type: research
Resolvin E1 Reduces Leukotriene B4-Induced Intracellular Calcium Increase and Mucin Secretion in Rat Conjunctival Goblet Cells.
Abstract Leukotriene B4 (LTB4) is a major proinflammatory mediator important in host defense whereas resolvins (Rvs) are produced during the resolution phase of inflammation. We determined the actions of both RvE1 and RvD1 on LTB4-induced responses of goblet cells cultured from rat conjunctiva. The responses measured were an increase in the intracellular [Ca2+] ([Ca2+]i) and high molecular weight glycoprotein secretion. Treatment with RvE1 or RvD1 for 30 min significantly blocked the LTB4-induced [Ca2+]i increase. The actions of RvE1 on LTB4-induced [Ca2+]i increase were reversed by siRNA for the RvE1 receptor and...
Source: The American Journal of Pathology - June 16, 2020 Category: Pathology Authors: Yang M, Bair JA, Hodges RR, Serhan CN, Dartt DA Tags: Am J Pathol Source Type: research
Viruses, Vol. 12, Pages 650: PRMT5 Is Required for Bovine Leukemia Virus Infection In Vivo and Regulates BLV Gene Expression, Syncytium Formation, and Glycosylation In Vitro
In conclusion, PRMT5 overexpression is related to the development of BLV infection with a high proviral load and lymphoma stage and PRMT5 inhibition enhances BLV gene expression. This is the first study to investigate the role of PRMT5 in BLV infection in vivo and in vitro and to reveal a novel function for a small-molecule compound in BLV-gp51 glycosylation processing.
Source: Viruses - June 16, 2020 Category: Virology Authors: Wlaa Assi Tomoya Hirose Satoshi Wada Ryosuke Matsuura Shin-nosuke Takeshima Yoko Aida Tags: Article Source Type: research
GSE143803 SREBF1 coordinates with master transcription factors in regulating lipid metabolism and cancer-promoting pathways in squamous cell carcinoma
Contributors : Liyan Li ; Qian Yang ; Yanyi Jiang ; Wei Yang ; Yuan Jiang ; Masaharu Hazawa ; Bo Zhou ; Xiang Li ; Sigal Gery ; Lingwen Ding ; Allen S Ho ; Zachary S Zumsteg ; Mingrong Wang ; Yanbing Ding ; Stephen J Freedland ; Liyan Xu ; Enmin Li ; H P Koeffler ; Dechen LinSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensWe profiled esophageal squamous cell carcinorma (ESCC) cell lines with chromatin immunoprecipitation sequencing (ChIP-Seq). Mathematically modeling was performed to establish (super)-enhancers landsca...
Source: GEO: Gene Expression Omnibus - June 15, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Homo sapiens Source Type: research
siRNA based drug design, quality, delivery and clinical translation
Publication date: Available online 13 June 2020Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Md Abdus Subhan, VP Torchilin
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 15, 2020 Category: Nanotechnology Source Type: research
Characterization of a Trichinella spiralis aminopeptidase and its participation in invasion, development and fecundity
AbstractATrichinella spiralis aminopeptidase (TsAP) has been identified in intestinal infectious larvae (IIL) and adult worms (AW), but its biological function in theT. spiralis life cycle is unknown. The aim of this study was to characterize TsAP and ascertain its functions in the invasion, development and fecundity ofT. spiralis. Recombinant TsAP (rTsAP) was expressed and purified. rTsAP has strong immunogenicity. qPCR and western blotting show that TsAP was transcribed and expressed at allT. spiralis lifecycle stages, but the expression level of TsAP mRNA and proteins at IIL and AW stages was obviously higher than those...
Source: Veterinary Research - June 15, 2020 Category: Veterinary Research Source Type: research
Sodium butyrate protects against oxidative stress in human nucleus pulposus cells via elevating PPAR γ-regulated Klotho expression.
Sodium butyrate protects against oxidative stress in human nucleus pulposus cells via elevating PPARγ-regulated Klotho expression. Int Immunopharmacol. 2020 Jun 15;85:106657 Authors: Liu X, Jiang C, Liu G, Wang P, Shi M, Yang M, Zhong Z, Ding S, Li Y, Liu B, Cao Y Abstract We investigated the involvement of klotho in the inhibition of oxidative stress by sodium butyrate (NaB) in human nucleus pulposus cells (NPCs). NPCs were pretreated with different concentrations of NaB for 2 h before stimulation with tert-butyl hydroperoxide (TBHP). NaB alleviated TBHP-induced oxidative injury in the NPCs, as evi...
Source: International Immunopharmacology - June 15, 2020 Category: Allergy & Immunology Authors: Liu X, Jiang C, Liu G, Wang P, Shi M, Yang M, Zhong Z, Ding S, Li Y, Liu B, Cao Y Tags: Int Immunopharmacol Source Type: research
ACSL4 is a predictive biomarker of sorafenib sensitivity in hepatocellular carcinoma.
This study may have important translational impacts in precise treatment of HCC. PMID: 32541921 [PubMed - as supplied by publisher]
Source: Acta Pharmacologica Sinica - June 15, 2020 Category: Drugs & Pharmacology Authors: Feng J, Lu PZ, Zhu GZ, Hooi SC, Wu Y, Huang XW, Dai HQ, Chen PH, Li ZJ, Su WJ, Han CY, Ye XP, Peng T, Zhou J, Lu GD Tags: Acta Pharmacol Sin Source Type: research
Aquaporin 1 affects pregnancy outcome and regulates aquaporin 8 and 9 expressions in the placenta
AbstractTo explore the effects of aquaporin (AQP) 1 on pregnancy outcome and the association between expression of AQP1 and other AQPs in the placenta and foetal membranes, the rate of copulatory plugs and pregnancy, amniotic fluid (AF) volume, osmolality and composition were determined inAQP1-knockout (AQP1−/−) mice at different gestational days (GD). The expression and location of AQP1 and other AQPs in the placenta and foetal membranes ofAQP1−/− mice, AQP1-siRNA transfected WISH cells and oligohydramnios patients were also detected. Compared to control mice,AQP1−/− mice exhibited reduced copulation plug and ...
Source: Cell and Tissue Research - June 15, 2020 Category: Cytology Source Type: research
TIR-domain-containing adapter-inducing interferon- β contributes to TLR3/TLR4 triggered apoptosis and inflammation in nucleus pulposus cells.
TIR-domain-containing adapter-inducing interferon-β contributes to TLR3/TLR4 triggered apoptosis and inflammation in nucleus pulposus cells. J Biol Regul Homeost Agents. 2020 Mar-Apr;34(3):445-455 Authors: Lv HL, Yu J, Pei JF, Wang HY, Guo ZL Abstract The senescence and degeneration of the intervertebral disc are closely related to the reduction of nucleus pulposus (NP) cells caused by apoptosis. TIR-domain-containing adapter-inducing interferon-β (TRIF) is an adapter for Toll-like receptors 3/4 (TLR3/4), which involves in cell apoptosis. The aim of this study is to detect the role of TRIF in the apo...
Source: Journal of Biological Regulators and Homeostatic Agents - June 14, 2020 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research
GSE132813 Master transcription factors form interconnected circuitry and orchestrate transcriptional networks in esophageal adenocarcinoma
Contributors : Jian Pan ; Tiago C Silva ; Nicole Gull ; Qian Yang ; Jasmine Plummer ; Stephanie Chen ; Kenji Daigo ; Takao Hamakubo ; Ling-Wen Ding ; Samuel J Klempner ; Benjamin P Berman ; H P Koeffler ; De-Chen LinSeries Type : Genome binding/occupancy profiling by high throughput sequencing ; Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiensWe profiled fresh-frozen esophageal tumor and normal samples and cell lines with chromatin immunoprecipitation sequencing (ChIP-Seq). Mathematically modeling was performed to establish (super)-enhancers landscapes and inter-connected transcriptional ci...
Source: GEO: Gene Expression Omnibus - June 14, 2020 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Expression profiling by high throughput sequencing Other Homo sapiens Source Type: research
Canonical Notch Signaling is Required for Bone Morphogenetic Protein ‐mediated Human Osteoblast Differentiation
BMP stimulation of bone ‐marrow derived human mesenchymal progenitor cells (hMSCs) increases Notch proteins via increased Notch ligand Jagged1 expression. Canonical Notch signaling is required for BMPinduced ALPL expression and osteoblastic commitment of hMSCs. Both BMP‐induced osteoblastogenesis and Notch‐induced os teoblastogenesis require Runx2. AbstractOsteoblast differentiation of bone ‐marrow derived human mesenchymal stem cells (hMSC) can be induced by stimulation with either canonical Notch ligand, Jagged1, or bone morphogenetic proteins (BMP). However, it remains elusive how these two pathways lead to the ...
Source: Stem Cells - June 14, 2020 Category: Stem Cells Authors: Yadav Wagley, Alessandra Chesi, Parker K. Acevedo, Sumei Lu, Andrew D. Wells, Matthew E. Johnson, Struan F. A. Grant, Kurt D. Hankenson Tags: Tissue ‐Specific Stem Cells Source Type: research
Fucoidan inhibits LPS-induced acute lung injury in mice through regulating GSK-3 β-Nrf2 signaling pathway.
This study showed fucoidan protected mice against LPS-induced ALI through inhibiting inflammatory and oxidative responses via regulating GSK-3β-Nrf2 signaling pathway. PMID: 32533502 [PubMed - as supplied by publisher]
Source: Archives of Pharmacal Research - June 13, 2020 Category: Drugs & Pharmacology Authors: Zhu DZ, Wang YT, Zhuo YL, Zhu KJ, Wang XZ, Liu AJ Tags: Arch Pharm Res Source Type: research
Methamphetamine induces GSDME-dependent cell death in hippocampal neuronal cells through the endoplasmic reticulum stress pathway.
Abstract Methamphetamine (METH) is an illegal amphetamine-typed psychostimulant that is abused worldwide and causes serious public health problems. METH exposure induces apoptosis and autophagy in neuronal cells. However, the role of pyroptosis in METH-induced neurotoxicity is still unclear. Here, we investigate whether pyroptosis is involved in METH-induced hippocampal neurotoxicity and the potential mechanisms of Endoplasmic reticulum (ER) stress in hippocampal neuronal cells. For this purpose, the expression levels of pyroptosis-related proteins, GSDMD and GSDME, were analyzed by immunoblotting and immunohistoc...
Source: Brain Research Bulletin - June 13, 2020 Category: Neurology Authors: Liu Y, Wen D, Gao J, Xie B, Yu H, Shen Q, Zhang J, Jing W, Cong B, Ma C Tags: Brain Res Bull Source Type: research
Prognostic significance of NAP1L1 expression in patients with early lung adenocarcinoma.
In this study, we investigated the relationship between NAP1L1 expression and the clinicopathological parameters and prognosis of non-small cell lung cancer patients. To this end, the expression of NAP1L1 in tumor samples was evaluated by immunohistochemistry. NAP1L1 expression was significantly associated with reduced differentiation (P = 0.00014), higher pathological TNM stages (P < 0.00001), lymph node metastasis (P < 0.00001), intrapulmonary metastasis (P = 0.02955), lymphatic invasion (P = 0.00019), vascular invasion (P = 0.00008) and poorer prognosis (P = 0.0008) of patients with adenocarcinoma. Moreover, multi...
Source: Biomed Res - June 12, 2020 Category: Research Authors: Nagashio R, Kuchitsu Y, Igawa S, Kusuhara S, Naoki K, Satoh Y, Ichinoe M, Murakumo Y, Saegusa M, Sato Y Tags: Biomed Res Source Type: research
Activin B signaling may promote the conversion of normal fibroblasts to scar fibroblasts
This study is to explore the molecular mechanism of benign bile duct hypertrophic scar formation. Differential proteins between the normal fibroblast (NFB) and scar fibroblast (SCFB) were screened by protein chip assay, and analyzed by pathway-enrichment analysis and function-enrichment analysis. The differential proteins were further tested by ELISA. SiRNA-Act B was transfected to SCFB to down-regulate the expression of Act B. NFB was incubated with rh-Act B. The cell apoptosis and cell cycle were determined by flow cytometry. The expression of Act B, Smad2/3, transforming growth factor-β1 (TGF-β1), endothelin-1 (ET-1)...
Source: Medicine - June 12, 2020 Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research
Co-delivery of gemcitabine prodrug along with anti NF-κB siRNA by tri-layer micelles can increase cytotoxicity, uptake and accumulation of the system in the cancers
Publication date: Available online 6 June 2020Source: Materials Science and Engineering: CAuthor(s): Parisa Norouzi, Mohsen Amini, Rassoul Dinarvand, Ehsan Arefian, Ehsan Seyed Jafari, Fatemeh Atyabi
Source: Materials Science and Engineering: C - June 12, 2020 Category: Materials Science Source Type: research
Resveratrol inhibits the malignant progression of hepatocellular carcinoma via MARCH1-induced regulation of PTEN/AKT signaling.
This study determined the molecular mechanisms underlying the antitumor effects of resveratrol in HCC. Resveratrol induced apoptosis and inhibited the proliferation, migration, and invasion of HCC cell lines (HepG2 and Hep3B). In addition, it inhibited MARCH1 and phospho-protein kinase B (p-AKT) expression but upregulated the phosphatase and tensin homolog deleted on chromosome 10 (PTEN) dose-dependently both in vitro and in vivo. MARCH1 knockdown by small interfering RNA (siRNA) also increased PTEN expression. Meanwhile, MK2206 (an AKT inhibitor) and bisperoxovanadium (BPV; a PTEN inhibitor) combined with resveratrol decr...
Source: Aging - June 12, 2020 Category: Biomedical Science Authors: Dai H, Li M, Yang W, Sun X, Wang P, Wang X, Su J, Wang X, Hu X, Zhao M Tags: Aging (Albany NY) Source Type: research
Occludin degradation makes brain microvascular endothelial cells more vulnerable to reperfusion injury in vitro
AbstractIntracerebral hemorrhage is the most dangerous complication in tPA thrombolytic therapy for ischemic stroke, which occurs as a consequence of endothelial cell death at the blood brain barrier (BBB) during thrombolytic reperfusion. We have previously shown that cerebral ischemia induced rapid occludin degradation and BBB disruption. Here we demonstrated an important role of occludin degradation in facilitating the evolution of ischemic endothelial cells towards death. Cultured brain microvascular endothelial cells (bEnd.3 cells) were exposed to oxygen ‐glucose deprivation (OGD) or incubated with occludin siRNA or ...
Source: Journal of Neurochemistry - June 12, 2020 Category: Neuroscience Authors: Yuan Zhang, Xiaofeng Li, Shanshan Qiao, Dexin Yang, Zongyang Li, Ji Xu, Weiping Li, Li Su, Wenlan Liu Tags: ORIGINAL ARTICLE Source Type: research
NF- κB/TWIST1 Mediates Migration and Phagocytosis of Macrophages in the Mice Model of Implant-Associated Staphylococcus aureus Osteomyelitis
Staphylococcus aureus (S. aureus) infection-induced osteomyelitis is a great challenge in clinic treatment. Identification of the essential genes and biological processes that are specifically changed in mononuclear cells at an early stage of S. aureus osteomyelitis is of great clinical significance. Based on transcriptional dataset GSE16129 available publicly, a bioinformatic analysis was performed to identify the differentially expressed genes of osteomyelitis caused by S. aureus infection. ERBB2, TWIST1, and NANOG were screened out as the most valuable osteomyelitis-related genes (OMRGs). A mice model of implant-associa...
Source: Frontiers in Microbiology - June 12, 2020 Category: Microbiology Source Type: research
DCLK1 inhibition attenuates tumorigenesis and improves chemosensitivity in esophageal squamous cell carcinoma by inhibiting β-catenin/c-Myc signaling.
In this study, real-time PCR and western blot analysis confirmed significant upregulation of DCLK1 expression in human ESCC tissues and cell lines. CCK-8 assay showed that transfection with siRNA against DCLK1 (si-DCLK1) markedly inhibited cell proliferation and colony formation in the ESCC cell lines Eca109 and TE1. Transwell assay revealed that si-DCLK1 transfection inhibited the migratory and invasive capacities of Eca109 and TE1 cells. Moreover, si-DCLK1 increased the chemosensitivity of these cells to cisplatin, as indicated by inhibited cell viability and colony formation, and increased ROS and apoptosis in cisplatin...
Source: Pflugers Archiv : European Journal of Physiology - June 12, 2020 Category: Physiology Authors: Zhang L, Zhou S, Guo E, Chen X, Yang J, Li X Tags: Pflugers Arch Source Type: research
Matrine suppresses advanced glycation end products-induced human coronary smooth muscle cells phenotype conversion by regulating endoplasmic reticulum stress-dependent Notch signaling.
Abstract Advanced glycation end products (AGEs) induce vascular smooth muscle cells (VSMCs) contractile-synthetic phenotypic conversion which plays roles in aggravated atherosclerosis in diabetes. Matrine has been proved to suppress AGEs-induced phenotypic conversion which is governed by Notch pathway. Endoplasmic reticulum stress was associated with Notch pathway. Cultured human coronary smooth muscle cells (HCSMCs) were incubated with AGE-BSA at 0, 5 and 10 μmol/l. Specific siRNA was used to silence Protein kinase RNA-like ER kinase (PERK). Matrine at 0, 0.5 and 1.0 mmol/l were used to pre-treat the cells. ...
Source: European Journal of Pharmacology - June 12, 2020 Category: Drugs & Pharmacology Authors: Zhao L, Cai H, Tang Z, Cui Q, Liu Z, Lu S Tags: Eur J Pharmacol Source Type: research
Cancers, Vol. 12, Pages 1548: Protein Stability of Pyruvate Kinase Isozyme M2 Is Mediated by HAUSP
ang-Hyun Baek The ubiquitin&ndash;proteasome system (UPS) is responsible for proteasomal degradation, regulating the half-life of the protein. Deubiquitinating enzymes (DUBs) are components of the UPS and inhibit degradation by removing ubiquitins from protein substrates. Herpesvirus-associated ubiquitin-specific protease (HAUSP) is one such deubiquitinating enzyme and has been closely associated with tumor development. In a previous study, we isolated putative HAUSP binding substrates by two-dimensional electrophoresis (2-DE) and identified them by matrix-assisted laser desorption-ionization time-of-flight mas...
Source: Cancers - June 12, 2020 Category: Cancer & Oncology Authors: Hae-Seul Choi Chang-Zhu Pei Jun-Hyeok Park Soo-Yeon Kim Seung-Yeon Song Gyeong-Jin Shin Kwang-Hyun Baek Tags: Article Source Type: research
‘Snail factors in testicular germ cell tumours and their regulation by the BMP4 signalling pathway’
ConclusionsThis is the first evidence that SNAI1 and SNAI2 are involved in human spermatogenesis, with independent functions. These outcomes demonstrate that SNAI1 and SNAI2 inhibition leads to loss of migratory and viability capacities in seminoma cells. These findings show the potential for therapeutic treatments targeting SNAIL or BMP4 signalling for patients with metastatic testicular germ cell tumours.
Source: Andrology - June 12, 2020 Category: Urology & Nephrology Authors: Diana J. Micati, Karthika Radhakrishnan, Julia C. Young, Ewa Rajpert ‐De Meyts, Gary R. Hime, Helen E. Abud, Kate L. Loveland Tags: ORIGINAL ARTICLE Source Type: research
Multifunctional magnetite nanoparticles to enable delivery of siRNA for the potential treatment of Alzheimer's.
This study aims to explore the ability of a multifunctional conjugate based on magnetite nanoparticles for the cellular delivery of siRNA against the expression of the BACE1 gene. We immobilized the siRNA strand on PEGylated magnetite nanoparticles and investigated the effects on biocompatibility and efficacy of the conjugation. Similarly, we co-immobilized the translocating protein OmpA on PEGylated nanoparticles to enhance cellular uptake and endosomal escape. BACE1 suppression was statistically significant in HFF-1 cells, without any presence of a cytotoxic effect. The delivery of the nanoconjugate was achieved through ...
Source: Drug Delivery - June 11, 2020 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research
ASAP Artificial Platelets for Efficient siRNA Delivery to Clear “Bad Cholesterol”
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.0c07559
Source: ACS Applied Materials and Interfaces - June 11, 2020 Category: Materials Science Authors: Yanfen Zhang †‡, Yuyan Qin†‡, Huan Li†‡, Qingsheng Peng‡, Ping Wang‡, Langyu Yang‡, Sheng Chen‡, Ming Li‡, Jijun Fu*‡§, Xiyong Yu*‡, and Lingmin Zhang*‡ Source Type: research
Inhibition of EZH2 and activation of ERR γ synergistically suppresses gastric cancer by inhibiting FOXM1 signaling pathway
ConclusionThe FOXM1 signaling pathway underlying the ERR γ-mediated gastric cancer suppression was identified. Furthermore, combined treatment with EZH2 inhibitor and ERRγ agonist synergistically suppressed GC progression by inhibiting this signaling pathway, suggesting its high potential in treating GC patients.
Source: Gastric Cancer - June 11, 2020 Category: Gastroenterology Source Type: research
HIF-1 α is involved in blood–brain barrier dysfunction and paracellular migration of bacteria in pneumococcal meningitis
AbstractBacterial meningitis is a deadly disease most commonly caused byStreptococcus pneumoniae, leading to severe neurological sequelae including cerebral edema, seizures, stroke, and mortality when untreated. Meningitis is initiated by the transfer ofS. pneumoniae from blood to the brain across the blood –cerebrospinal fluid barrier or the blood–brain barrier (BBB). The underlying mechanisms are still poorly understood. Current treatment strategies include adjuvant dexamethasone for inflammation and cerebral edema, followed by antibiotics. The success of dexamethasone is however inconclusive, ne cessitating new ther...
Source: Acta Neuropathologica - June 11, 2020 Category: Neurology Source Type: research
Diabetes mellitus exacerbates post ‐myocardial infarction heart failure by reducing sarcolipin promoter methylation
ConclusionsDiabetes increases the vulnerability of STEMI patients to post ‐MI HF by down‐regulating SLN promoter methylation, which further regulates SERCA2a activity via increasing cardiac SLN expression.
Source: ESC Heart Failure - June 11, 2020 Category: Cardiology Authors: Zhongwei Liu, Yong Zhang, Chuan Qiu, Haitao Zhu, Shuo Pan, Hao Jia, Hongyan Kang, Gongchang Guan, Rutai Hui, Ling Zhu, Junkui Wang Tags: Original Research Article Source Type: research
Enhancement of Liposomal Plasmid DNA and siRNA Delivery by Itraconazole through Intracellular Cholesterol Accumulation
ConclusionsItraconazole enhanced gene delivery of pDNA and siRNA, and it can be used to potentiate nucleic acid therapeutics.
Source: Pharmaceutical Research - June 11, 2020 Category: Drugs & Pharmacology Source Type: research
Molecular characterization of ovary-specific gene Mrfem-1 and siRNA-mediated regulation on targeting Mrfem-1 in the giant freshwater prawn, Macrobrachium rosenbergii.
In this study, a fem-1 gene (Mrfem-1) was first isolated from the giant freshwater prawn Macrobrachium rosenbergii. The full-length cDNA of Mrfem-1 was 2607 bp long, containing an open reading frame encoding 615 amino acids, and presenting eight ankyrin repeats. The full-length cDNA has been submitted to GenBank with the accession no. MT160093. According to the RT-PCR results, Mrfem-1 was exclusively expressed in the ovary. The expression level of Mrfem-1 had increased with ovarian maturation and reached the highest peak at vitellogenic stage. In situ hybridization results showed that positive signals were concentrated in ...
Source: Gene - June 11, 2020 Category: Genetics & Stem Cells Authors: Zhou LX, Liu X, Ye BQ, Liu Y, Tan SP, Ma KY, Qiu GF Tags: Gene Source Type: research
Molecules, Vol. 25, Pages 2714: Conjugation of Doxorubicin to siRNA Through Disulfide-based Self-immolative Linkers
Debart Co-delivery systems of siRNA and chemotherapeutic drugs have been developed as an attractive strategy to optimize the efficacy of chemotherapy towards cancer cells with multidrug resistance. In these typical systems, siRNAs are usually associated to drugs within a carrier but without covalent interactions with the risk of a premature release and degradation of the drugs inside the cells. To address this issue, we propose a covalent approach to co-deliver a siRNA-drug conjugate with a redox-responsive self-immolative linker prone to intracellular glutathione-mediated disulfide cleavage. Herein, we report the use...
Source: Molecules - June 11, 2020 Category: Chemistry Authors: Florian Gauthier Jean-R émi Bertrand Jean-Jacques Vasseur Christelle Dupouy Fran çoise Debart Tags: Article Source Type: research
bFGF promotes neurological recovery from neonatal hypoxic –ischemic encephalopathy by IL‐1β signaling pathway‐mediated axon regeneration
ConclusionsWe showed that IL ‐1β‐mediated axon regeneration underlie the mechanism of bFGF for the treatment of HIBD in neonatal rats. Results from this study would provide insights and molecular basis for future therapeutics in treating HIBD.
Source: Brain and Behavior - June 11, 2020 Category: Neurology Authors: Zheng Ma, Fang Wang, Lu ‐Lu Xue, Ying‐Jie Niu, Yue Hu, Zhang‐Yu Su, Jin Huang, Rui‐Ze Niu, Ting‐Hua Wang, Ying‐Chun Ba, Liu‐Lin Xiong, Xue Bai Tags: ORIGINAL RESEARCH Source Type: research
Effect of siRNA-induced Atg7 gene silencing on the sensitivity of ovarian cancer SKOV3 cells to cisplatin.
Authors: Zhang X, Wang LL, Wang B, Liu HL, Zhang J, Li YH, Wang LH Abstract Ovarian cancer is one of the most common types of gynecological malignant tumors. A proclivity for, or the development of chemoresistance severely affects treatment efficacy for ovarian cancer. Herein we found that as concentrations of cisplatin (DDP) used in SKOV3 cells increased, expression of intracellular reactive oxygen species (ROS) increased as did amounts of proteins of Beclin-1 and Autophagy-Related Gene 7 (Atg7) whereas in contrast, expression of P62 protein decreased gradually. Expression of Atg7 protein in SKOV3 cells in the siR...
Source: American Journal of Translational Research - June 10, 2020 Category: Research Tags: Am J Transl Res Source Type: research
Molecules, Vol. 25, Pages 2692: Lipid and Polymer-Based Nanoparticle siRNA Delivery Systems for Cancer Therapy
les RNA interference (RNAi) uses small interfering RNAs (siRNAs) to mediate gene-silencing in cells and represents an emerging strategy for cancer therapy. Successful RNAi-mediated gene silencing requires overcoming multiple physiological barriers to achieve efficient delivery of siRNAs into cells in vivo, including into tumor and/or host cells in the tumor micro-environment (TME). Consequently, lipid and polymer-based nanoparticle siRNA delivery systems have been developed to surmount these physiological barriers. In this article, we review the strategies that have been developed to facilitate siRNA survival in the ci...
Source: Molecules - June 10, 2020 Category: Chemistry Authors: Francesco Mainini Michael R. Eccles Tags: Review Source Type: research
Exosomes-coated bcl-2 siRNA inhibits the growth of digestive system tumors both in vitro and in vivo.
CONCLUSION: ExosiBcl-2 has potential to be developed as a novel anticancer agent. PMID: 32531356 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - June 9, 2020 Category: Biochemistry Authors: Tao H, Xu H, Zuo L, Li C, Qiao G, Guo M, Zheng L, Leitgeb M, Lin X Tags: Int J Biol Macromol Source Type: research
Melanoma Cancer Immunotherapy Using PD-L1 siRNA and Imatinib Promotes Cancer-Immunity Cycle
ConclusionOverall, results revealed that the tumors treated with siPDIN restored the immunity of CTLs by potentially inhibiting the immune checkpoint interactions, suppressed the mTOR signaling pathway and exhibited an enhanced anticancer efficacy in melanoma.
Source: Pharmaceutical Research - May 31, 2020 Category: Drugs & Pharmacology Source Type: research
Dual-targeting RNA nanoparticles for efficient delivery of polymeric siRNA to cancer cells
Chem. Commun., 2020, Advance Article DOI: 10.1039/D0CC01848A, CommunicationTaehyung Kim, Ha Na Hyun, Roun Heo, Keonwook Nam, Kyungjik Yang, Young Min Kim, Yoon Suk Lee, Jae Yoon An, Jae Hyung Park, Ki Young Choi, Young Hoon Roh A new dual-targeting polymeric siRNA nanoparticle (Dual-PSNP) was developed. The Dual-PSNP showed significantly enhanced cancer-specific intracellular delivery, gene regulation efficacy, and apoptosis-mediated cytotoxicity through additive receptor-mediated interactions of the two ligands. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this ...
Source: RSC - Chem. Commun. latest articles - May 29, 2020 Category: Chemistry Authors: Taehyung Kim Source Type: research
Dual-targeting RNA nanoparticles for efficient delivery of polymeric siRNA to cancer cells.
Abstract A new dual-targeting polymeric siRNA nanoparticle (Dual-PSNP) was developed via multiple processes: rolling circle transcription, condensation, electrostatic deposition, and click chemistry. The Dual-PSNP showed significantly improved cancer-specific intracellular delivery, gene knockdown efficacy, and apoptosis-mediated cytotoxicity through additive receptor-mediated interactions of the two ligands. PMID: 32463029 [PubMed - as supplied by publisher]
Source: Chemical Communications - May 28, 2020 Category: Chemistry Authors: Kim T, Hyun HN, Heo R, Nam K, Yang K, Kim YM, Lee YS, An JY, Park JH, Choi KY, Roh YH Tags: Chem Commun (Camb) Source Type: research
Comparison of effects of dsRNA and siRNA RNA interference on insulin-like androgenic gland gene (IAG) in red swamp crayfish Procambarus clarkii.
In this study, the effective sequence of siRNA and optimal injection dose were determined, and the effects of RNAi using dsRNA, siRNA, and long-term RNAi were investigated. The results showed that the doses of 0.5 and 1µg/g of body weight of IAG-siRNA3 produced significantly better inhibition than 0.1µg/g. qPCR assays showed that both dsRNA and siRNA silenced the IAG expression in five tissues (brain, ventral nerve cord, androgenic gland, testis, and vas deferens) in adult P. clarkii, with the effectiveness decreasing over time, inhibiting the production of spermatid. dsRNA exhibited a longer interference effect than siR...
Source: Gene - May 16, 2020 Category: Genetics & Stem Cells Authors: Ge HL, Tan K, Shi LL, Sun R, Wang WM, Li YH Tags: Gene Source Type: research
GSE148429 The CCR4-NOT complex component NOT1 regulates RNA-directed DNA methylation and transcriptional silencing by facilitating Pol IV-dependent siRNA production small RNA-seq
Contributor : Xin-jian HeSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Arabidopsis thalianaSmall interfering RNAs (siRNAs) are required for establishment and maintenance of DNA methylation through RNA-directed DNA methylation (RdDM) pathway in plants. While the siRNA biogenesis is well known, relatively little is known about how the process is regulated. By a forward genetic screen in Arabidopsis thaliana, we identified a mutant defective in NOT1 and demonstrated that NOT1 is required for transcriptional silencing at RdDM target genomic loci. Furthermore, we revealed that NOT1 is required f...
Source: GEO: Gene Expression Omnibus - May 15, 2020 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Arabidopsis thaliana Source Type: research
GSE148428 The CCR4-NOT complex component NOT1 regulates RNA-directed DNA methylation and transcriptional silencing by facilitating Pol IV-dependent siRNA production Bisulfite-seq
Contributor : Xin-jian HeSeries Type : Methylation profiling by high throughput sequencingOrganism : Arabidopsis thalianaSmall interfering RNAs (siRNAs) are required for establishment and maintenance of DNA methylation through RNA-directed DNA methylation (RdDM) pathway in plants. While the siRNA biogenesis is well known, relatively little is known about how the process is regulated. By a forward genetic screen in Arabidopsis thaliana, we identified a mutant defective in NOT1 and demonstrated that NOT1 is required for transcriptional silencing at RdDM target genomic loci. Furthermore, we revealed that NOT1 is required for ...
Source: GEO: Gene Expression Omnibus - May 15, 2020 Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Arabidopsis thaliana Source Type: research
Conjugating pyrene onto PNA-based fluorescent probes for improved detection selectivity toward double-stranded siRNA
We report on the design of new siRNA-binding fluorescent probes with the improved detection selectivity toward intact double-stranded siRNAs over single-stranded forms by the conjugation of pyrene unit into thiazole... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic and Biomolecular Chemistry - May 13, 2020 Category: Molecular Biology Authors: Yusuke Sato Source Type: research
Lipid-encapsulated siRNA for hepatocyte-directed treatment of advanced liver disease
Cell Death & Disease, Published online: 11 May 2020; doi:10.1038/s41419-020-2571-4Lipid-encapsulated siRNA for hepatocyte-directed treatment of advanced liver disease
Source: Cell death and disease - May 11, 2020 Category: Internal Medicine Authors: Marius Maximilian Woitok Miguel Eugenio Zoubek Dennis Doleschel Matthias Bartneck Mohamed Ramadan Mohamed Fabian Kie ßling Wiltrud Lederle Christian Trautwein Francisco Javier Cubero Source Type: research
In Vitro Screening and Transfection Concentration Optimization of Cynomolgus Monkey I κBα-siRNA.
Conclusion: It proves that the siRNA targeting the GCACTTAGCCTCTATCCAT of IκBα gene is the valid sequence to suppress cynomolgus monkey IκBα expression of CM cells and TM cells by RNAi. 10 nM is the optimal transfection concentration. PMID: 32377413 [PubMed]
Source: Journal of Ophthalmology - May 8, 2020 Category: Opthalmology Tags: J Ophthalmol Source Type: research
Characterization of Novel Aptamers Specifically Directed to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV)-Infected Cells for Mediating Targeted siRNA Delivery
In this study, we generated three aptamers against red-spotted grouper nervous necrosis virus (RGNNV)-infected grouper brain (GB) cells using the Cell-SELEX (cell based-systematic evolution of ligands by exponential enrichment) technology. The selected aptamers formed stable stem-loop structures, and could specifically recognize RGNNV-infected GB cells, with calculated dissociation constants (Kd) of 27.96, 29.3, and 59.5 nM for aptamers GBN2, GBN10, and GBN34, respectively. They also recognized RGNNV-infected brain tissues. The three aptamers were non-toxic and showed antiviral activities both in vitro and in vivo. Fluores...
Source: Frontiers in Microbiology - April 30, 2020 Category: Microbiology Source Type: research
A Tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in < i > C. elegans < /i >
piRNAs play a critical role in the regulation of transposons and other germline genes. InCaenorhabditis elegans, regulation of piRNA target genes is mediated by themutator complex, which synthesizes high levels of siRNAs through the activity of an RNA-dependent RNA polymerase. However, the steps between mRNA recognition by the piRNA pathway and siRNA amplification by themutatorcomplex are unknown. Here, we identify the Tudor domain protein, SIMR-1, as acting downstream of piRNA production and upstream ofmutator complex-dependent siRNA biogenesis. Interestingly, SIMR-1 also localizes to distinct subcellular foci adjacent to...
Source: eLife - April 27, 2020 Category: Biomedical Science Tags: Chromosomes and Gene Expression Source Type: research
siRNA-Mediated MrIAG Silencing Induces Sex Reversal in Macrobrachium rosenbergii.
Abstract The insulin-like androgenic gland (IAG) gene is well known in male crustacean, and it is a key regulator in male sexual differentiation and maintaining the male sexual characteristic. The neo-female can be produced by silencing the MrIAG (Macrobrachium rosenbergii Insulin-like Androgenic Gland) in male Macrobrachium rosenbergii. This is the first time to use siRNA approach to silenced MrIAG in male M. rosenbergii. In the current study, the optimal injection dosage to achieve sex reversal is 0.5 μg/g body weight. After MrIAG silencing, the expression level of Dmrt11e, Dmrt99b, MRPINK, Mrr, Sxl1, and Sxl2...
Source: Marine Biotechnology - April 27, 2020 Category: Biotechnology Authors: Tan K, Zhou M, Jiang H, Jiang D, Li Y, Wang W Tags: Mar Biotechnol (NY) Source Type: research
Dual targeting RNA nanoparticles for efficient delivery of polymeric siRNA to cancer cells
Chem. Commun., 2020, Accepted Manuscript DOI: 10.1039/D0CC01848A, CommunicationTaehyung Kim, Hana Hyun, Roun Heo, Keonwook Nam, Kyungjik Yang, Young Min Kim, Yoon Suk Lee, Jae Yoon An, Jae Hyung Park, KI YOUNG CHOI, Young Hoon Roh A new dual-targeting polymeric siRNA nanoparticle (Dual-PSNP) was developed via multiple processes; rolling circle transcription, condensation, electrostatic deposition, and click chemistry. The Dual-PSNP showed significantly improved cancer specific intracellular delivery,... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - April 25, 2020 Category: Chemistry Authors: Taehyung Kim Source Type: research
Exploring Sirna Umpired Nanogels: A Tale Of Barrier Combating Carrier.
Abstract Potential short interfering RNAs (siRNA) modulating gene expression are emerged as a novel therapeutic arsenal against a wide range of maladies and disorders containing cancer, viral infections, bacterial ailments and metabolic snags at molecular level. Nanogel in current medicinal era displayed a comprehensive range of significant drug delivery prospectives. Biodegradation, swelling and de-swelling tendency, pH sensitive drug release, thermo-sensitivity are some of the renowned associated benefits of nanogel drug delivery system. Global researches have also showed that nanogel system significantly target...
Source: Current Pharmaceutical Design - April 17, 2020 Category: Drugs & Pharmacology Authors: Kashaw SK, Sahu P, Rajoriya V, Jana P, Kashaw V, Sau S, Iyer AK Tags: Curr Pharm Des Source Type: research