Query: siRNA

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Total 15136 results found since Jan 2013.

Lipodendriplexes: A promising nanocarrier for enhanced gene delivery with minimal cytotoxicity.
In this study, we developed an advanced variant of the dendriplexes by encapsulating them within liposomes to enhance their gene delivery efficiency. This liposome encapsulated dendriplex system can further reduce unwanted cytotoxicity and enhance cellular uptake of nucleic acids. A broad range of lipid combinations were used to optimize the lipodendriplexes in terms of their physicochemical characteristics including size, shape and zeta potential. The optimized lipodendriplexes were tested for pDNA transfection, in vitro cell viability, cellular uptake, siRNA mediated knockdown, hemocompatibility, metastatic progression a...
Source: European Journal of Pharmaceutics and Biopharmaceutics - December 24, 2018 Category: Drugs & Pharmacology Authors: Tariq I, Reddy Pinnapireddy S, Duse L, Yasir Ali M, Ali S, Umair Amin M, Goergen N, Jedelská J, Schäfer J, Bakowsky U Tags: Eur J Pharm Biopharm Source Type: research

Inhibiting expression of HSP60 and TLR4 attenuates paraquat-induced microglial inflammation
Publication date: Available online 22 December 2018Source: Chemico-Biological InteractionsAuthor(s): Xin-Lei Li, Yong-Ling Wang, Jing Zheng, Yang Zhang, Xiao-Feng ZhangAbstractAccumulating evidences suggest that heat shock protein 60 (HSP60) and toll-like receptor 4 (TLR4) are involved in triggering inflammatory response in microglia. Paraquat (PQ) evokes microglial inflammation by up-regulating expression of HSP60- TLR4-myeloid differentiation factor 88 (Myd88)-nuclear factor-kappa B (NF-κB) in vitro. The aim of this study is to investigate the potential modulatory roles of HSP60 and TLR4 in PQ-induced inflammation. Befo...
Source: Chemico Biological Interactions - December 22, 2018 Category: Biochemistry Source Type: research

MiR-194-5p inhibits cell migration and invasion in bladder cancer by targeting E2F3.
CONCLUSIONS: MiR-194-5p directly targets E2F3 and inhibits cell migration and invasion in BC. PMID: 30570877 [PubMed - in process]
Source: Journal of B.U.ON. - December 22, 2018 Category: Cancer & Oncology Tags: J BUON Source Type: research

Circ-ZNF609 promotes migration of colorectal cancer by inhibiting Gli1 expression via microRNA-150.
CONCLUSIONS: Circ-ZNF609 regulates Gli1 expression via microRNA-150, thus affecting the migration of colorectal cancer. PMID: 30570857 [PubMed - in process]
Source: Journal of B.U.ON. - December 22, 2018 Category: Cancer & Oncology Tags: J BUON Source Type: research

Heat shock protein 70 as a supplementary receptor facilitates enterovirus 71infections in vitro
In this study, siRNA interference technique and transgenic technique were used to investigate the interaction between HSP70 and EV71 virus. The result demonstrated that the cell surface HSP70 is not essential for EV71 infection but helps the initial binding of virus to host cells and that multiple receptors are involved during EV71 infection. In addition, HSP70 was upregulated in human neuroblastoma cells (SK-N-SH) infected with EV71.Graphical abstractEnterovirus 71 (EV71) is a causative agent of hand food and mouth disease (HFMD) in young children and it can cause the damage of nervous cells, cytokine storm and toxic subs...
Source: Microbial Pathogenesis - December 22, 2018 Category: Infectious Diseases Source Type: research

Disruption of MAPK1 expression in the ERK signalling pathway and the RUNX1 ‑RUNX1T1 fusion gene attenuate the differentiation and proliferation and induces the growth arrest in t(8;21) leukaemia cells.
Disruption of MAPK1 expression in the ERK signalling pathway and the RUNX1‑RUNX1T1 fusion gene attenuate the differentiation and proliferation and induces the growth arrest in t(8;21) leukaemia cells. Oncol Rep. 2018 Dec 12;: Authors: Bashanfer SAA, Saleem M, Heidenreich O, Moses EJ, Yusoff NM Abstract The t(8;21) translocation is one of the most frequent chromosome abnormalities associated with acute myeloid leukaemia (AML). This abberation deregulates numerous molecular pathways including the ERK signalling pathway among others. Therefore, the aim of the present study was to investigate the gene ex...
Source: Oncology Reports - December 22, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Naringin targets Zeb1 to suppress osteosarcoma cell proliferation and metastasis.
Abstract Naringin, a citrus bioflavonoid, has anti-inflammatory actions and cardio- and neuroprotective effects. In addition, naringin exhibits multiple antitumor actions in several cancer types, including osteosarcoma, the most common type of bone cancer. Here, we show that naringin inhibits proliferation and invasion and induces apoptosis in human osteosarcoma cells by inhibiting zinc finger E-box binding homeobox 1 (Zeb1), a transcriptional repressor of epithelial differentiation involved in tumor metastasis. Our expression analyses confirm that Zeb1 is highly expressed in osteosarcoma specimens and cell lines....
Source: Aging - December 22, 2018 Category: Biomedical Science Authors: Ming H, Chuang Q, Jiashi W, Bin L, Guangbin W, Xianglu J Tags: Aging (Albany NY) Source Type: research

Leishmania donovani evades Caspase 1 dependent host defense mechanism during infection.
Abstract Significant advances have been made in understanding the regulation of inflammasomes and its involvement in innate immunity during pathogenic infections. Inflammasome activation is a tightly regulated process that provides defense against pathogenic infection and important for inflammatory response. Very few studies on the involvement of NLRP3 inflammasome protein complex have been reported in leishmanial infections with contradictory results and without much mechanistic insights. However, the role of NLRP3 inflammasome and its components has not been well deciphered in Leishmania donovani infection. Here...
Source: International Journal of Biological Macromolecules - December 22, 2018 Category: Biochemistry Authors: Saha G, Khamar BM, Singh OP, Sundar S, Dubey VK Tags: Int J Biol Macromol Source Type: research

Inhibiting expression of HSP60 and TLR4 attenuates paraquat-induced microglial inflammation.
Abstract Accumulating evidences suggest that heat shock protein 60 (HSP60) and toll-like receptor 4 (TLR4) are involved in triggering inflammatory response in microglia. Paraquat (PQ) evokes microglial inflammation by up-regulating expression of HSP60- TLR4-myeloid differentiation factor 88 (Myd88)-nuclear factor-kappa B (NF-κB) in vitro. The aim of this study is to investigate the potential modulatory roles of HSP60 and TLR4 in PQ-induced inflammation. Before treated with PQ, microglia BV2 cells were pretreated using siRNA to knockdown HSP60 or with specific inhibitor to inhibit TLR4 expression. Expression of TL...
Source: Chemico-Biological Interactions - December 22, 2018 Category: Molecular Biology Authors: Li XL, Wang YL, Zheng J, Zhang Y, Zhang XF Tags: Chem Biol Interact Source Type: research

ASAP Regulation of Ca < sup > 2+ < /sup > Signaling for Drug-Resistant Breast Cancer Therapy with Mesoporous Silica Nanocapsule Encapsulated Doxorubicin/siRNA Cocktail
ACS NanoDOI: 10.1021/acsnano.8b05639
Source: ACS Nano - December 21, 2018 Category: Nanotechnology Authors: Shu Wang, Xi Liu, Shizhu Chen, Zhirong Liu, Xiaodi Zhang, Xing-Jie Liang, Linlin Li Source Type: research

Regulation of Kv11.1 potassium channel C-terminal isoform expression by the RNA-binding proteins HuR and HuD RNA
In this study, using HEK293 cells and reporter gene expression, pulldown assays, and RNase protection assays, we identified the RNA-binding proteins Hu antigen R (HuR) and Hu antigen D (HuD) as regulators of Kv11.1 isoform expression. We show that HuR and HuD inhibit activity at the intron 9 polyadenylation site. When co-expressed with the KCNH2 gene, HuR and HuD increased levels of the Kv11.1a isoform and decreased the Kv11.1a-USO isoform in the RNase protection assays and immunoblot analyses. In patch clamp experiments, HuR and HuD significantly increased the Kv11.1 current. siRNA-mediated knockdown of HuR protein decrea...
Source: Journal of Biological Chemistry - December 21, 2018 Category: Chemistry Authors: Qiuming Gong, Matthew R. Stump, Zhengfeng Zhou Tags: Membrane Biology Source Type: research

Enhanced interaction between SEC2 mutant and TCR V{beta} induces MHC II-independent activation of T cells via PKC{theta}/NF-{kappa}B and IL-2R/STAT5 signaling pathways Cell Biology
In this study, we found that in contrast to SEC2, ST-4 could induce murine CD4+ T-cell proliferation in a Vβ8.2- and Vβ8.3-specific manner in the absence of MHC II+ antigen-presenting cells (APCs). Furthermore, although IL-2 secretion in response to either SEC2 or ST-4 stimulation was accompanied by up-regulation of protein kinase Cθ (PKCθ), inhibitor of κB (IκB), α and β IκB kinase (IKKα/β), IκBα, and NF-κB in mouse splenocytes, only ST-4 could activate CD4+ T cells in the absence of MHC II+ APCs through the PKCθ/NF-κB signaling pathway. The PKCθ inhibitor AEB071 significantly suppressed SEC2/ST-4–induc...
Source: Journal of Biological Chemistry - December 21, 2018 Category: Chemistry Authors: Xuanhe Fu, Mingkai Xu, Yubo Song, Yongqiang Li, Huiwen Zhang, Jinghai Zhang, Chenggang Zhang Tags: Signal Transduction Source Type: research

Glucose-6-phosphate dehydrogenase deficiency increases cell adhesion molecules and activates human monocyte-endothelial cell adhesion: Protective role of l-cysteine.
Abstract Glucose-6-phosphate dehydrogenase is a major enzyme that supplies the reducing agent nicotinamide adenine dinucleotide phosphate hydrogen (NADPH), which is required to recycle oxidized/glutathione disulfide (GSSH) to reduced glutathione (GSH). G6PD-deficient cells are susceptible to oxidative stress and a deficiency of GSH. Endothelial dysfunction is characterized by the loss of nitric oxide (NO) bioavailability, which regulates leukocyte adhesion to endothelium. G6PD-deficient endothelial cells (EC) demonstrate a reduced expression of endothelial nitric oxide synthase (eNOS) and NO levels along with redu...
Source: Archives of Biochemistry and Biophysics - December 21, 2018 Category: Biochemistry Authors: Parsanathan R, Jain SK Tags: Arch Biochem Biophys Source Type: research

The suppressive effect of co-inhibiting PD-1 and CTLA-4 expression on H22 hepatomas in mice.
Conclusion: Co-inhibiting the expressions of PD-1 and CTLA-4 can effectively suppress the growth of H22 hepatoma and promote the apoptosis of tumor cells in mice. Blocking PD-1 and CTLA-4 can improve the vitality of T cells, and improve the immune environment and response. PMID: 30564277 [PubMed - in process]
Source: Cellular and Molecular Biology Letters - December 20, 2018 Category: Biochemistry Authors: Liang L, Ge K, Zhang F, Ge Y Tags: Cell Mol Biol Lett Source Type: research

MicroRNA-34a attenuates VEGF-mediated retinal angiogenesis via targeting the Notch1.
Abstract Pathological angiogenesis in retina is main pathogenesis of ocular diseases closely associated with vision loss. Present work aimed to investigate effects of microRNA-34a (miR-34a) and its potential target Notch1 on retinal angiogenesis. For this objective, we employed oxygen-induced retinopathy (OIR) rats and human retinal microvascular endothelial cells (HRMECs) stimulated with vascular endothelial growth factors (VEGF). H&E staining, western blot for VEGF and immunofluorescence staining for CD31 were performed to verify establishment of OIR model. We observed down-regulation of miR-34a and up-regul...
Source: Biochemistry and Cell Biology - December 20, 2018 Category: Biochemistry Authors: Shi S, Jin Y, Song H, Chen X Tags: Biochem Cell Biol Source Type: research

Auto-fluorescent polymer nanotheranostics for self-monitoring of cancer therapy via triple-collaborative strategy.
Abstract Aberrant regulation of angiogenesis supply sufficient oxygen and nutrients to exacerbate tumor progression and metastasis. Taking this hallmark of cancer into account, reported here is a self-monitoring and triple-collaborative therapy system by auto-fluorescent polymer nanotheranostics which could be concurrently against angiogenesis and tumor cell growth by combining the benefits of anti-angiogenesis, RNA interfere and photothermal therapy (PTT). Auto-fluorescent amphiphilic polymer polyethyleneimine-polylactide (PEI-PLA) with positive charge can simultaneously load hydrophobic antiangiogenesis agent co...
Source: Biomaterials - December 20, 2018 Category: Materials Science Authors: Shao L, Li Q, Zhao C, Lu J, Li X, Chen L, Deng X, Ge G, Wu Y Tags: Biomaterials Source Type: research

Secretory immunoglobulin A induces human lung fibroblasts to produce inflammatory cytokines and undergo activation.
This article is protected by copyright. All rights reserved. PMID: 30570135 [PubMed - as supplied by publisher]
Source: Clinical and Developmental Immunology - December 20, 2018 Category: Allergy & Immunology Authors: Arakawa S, Suzukawa M, Watanabe K, Kobayashi K, Matsui H, Nagai H, Nagase T, Ohta K Tags: Clin Exp Immunol Source Type: research

Opposite effects of Drosophila C3PO on gene silencing mediated by esi-2.1 and miRNA-bantam.
Abstract Translin/TRAX complex, also named as C3PO, is evolutionarily conserved and participates in diverse cellular processes in different organisms from yeast to human. C3PO plays a critical role in the activation of RNA-induced silencing complexes by promoting the unwinding and degradation of passenger strand of exogenous siRNAs (exo-siRNAs) in Drosophila and human. Moreover, human C3PO (hC3PO) has been found to broadly repress miRNAs by degrading miRNA precursors. However, the effect of Drosophila melanogaster C3PO (dmC3PO) on endogenous siRNA (endo-siRNA) and miRNA pathways remains unknown. Here, we found tha...
Source: Acta Biochimica et Biophysica Sinica - December 20, 2018 Category: Biochemistry Authors: Liu Y, Fang Y, Liu Y, Wang Z, Lyu B, Hu Y, Zhou X Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Cancers, Vol. 11, Pages 3: ATM Dependent DUSP6 Modulation of p53 Involved in Synergistic Targeting of MAPK and p53 Pathways with Trametinib and MDM2 Inhibitors in Cutaneous Melanoma
John Lunec MAPK and p14ARF&amp;ndash;MDM2&amp;ndash;p53 pathways are critical in cutaneous melanomas. Here, synergistic combination of the MEK inhibitor, trametinib, with MDM2 inhibitors, nutlin-3/RG7388/HDM201, and the mechanistic basis of responses, for BRAFV600E and p53WT melanoma cells, are reported. The combination treatments induced higher levels of p53 target gene transcripts and protein products, resulting in increased cell cycle arrest and apoptosis compared with MDM2 inhibitors alone, suggesting trametinib synergized with MDM2 inhibitors via upregulation of p53-dependent pathways. In addition, DUSP6 ...
Source: Cancers - December 20, 2018 Category: Cancer & Oncology Authors: Chiao-En Wu Tsin Shue Koay Arman Esfandiari Yi-Hsuan Ho Penny Lovat John Lunec Tags: Article Source Type: research

Exosomes derived from siRNA against GRP78 modified bone-marrow-derived mesenchymal stem cells suppress Sorafenib resistance in hepatocellular carcinoma
Sorafenib is an effective clinical drug in therapy of hepatocellular carcinoma, having led to improved prognosis in hepatocellular carcinoma patients. However acquired resistance is still being encountered. So...
Source: Journal of Nanobiotechnology - December 20, 2018 Category: Nanotechnology Authors: Hongdan Li, Cheng Yang, Yijie Shi and Liang Zhao Tags: Research Source Type: research

Glyoxalase 1 gene is highly expressed in basal-like human breast cancers and contributes to survival of ALDH1-positive breast cancer stem cells.
In this study, we found that GLO1 gene expression correlates with neoplasm histologic grade (χ 2 test, p = 0.002) and is elevated in human basal-like breast cancer tissues. Approximately 90% of basal-like cancers were grade 3 tumors highly expressing both GLO1 and the cancer stem cell marker ALDH1A3. ALDH1high cells derived from the MDA-MB 157 and MDA-MB 468 human basal-like breast cancer cell lines showed elevated GLO1 activity. GLO1 inhibition using TLSC702 suppressed ALDH1high cell viability as well as the formation of tumor-spheres by ALDH1high cells. GLO1 knockdown using specific siRNAs also suppressed ALDH1high cell...
Source: Oncotarget - December 19, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Reduction of PGRN increased fibrosis during skin wound healing in mice.
Authors: Li SS, Zhang MX, Wang Y, Wang W, Zhao CM, Sun XM, Dong GK, Li ZR, Yin WJ, Zhu B, Cai HX Abstract Progranulin (PGRN) is a multi-functional growth factor known to be involved in regulating of development, cell cycle progression, cell motility, tumorigenesis and angiogenesis. Research has revealed that PGRN is a crucial mediator of skin wound healing. Nonetheless, the role of PGRN in the fibrosis process of cutaneous wound healing has not been identified. In the present study, mice with excisional wounds were treated with si-m-PGRN or physiological saline. We observed the expression of PGRN in intact and post...
Source: Histology and Histopathology - December 19, 2018 Category: Cytology Tags: Histol Histopathol Source Type: research

Th1 cytokines TNF- α and IFN-γ promote corticosteroid resistance in developing human airway smooth muscle.
Th1 cytokines TNF-α and IFN-γ promote corticosteroid resistance in developing human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol. 2019 Jan 01;316(1):L71-L81 Authors: Britt RD, Thompson MA, Sasse S, Pabelick CM, Gerber AN, Prakash YS Abstract Corticosteroids (CSs) are commonly used to manage wheezing and asthma in pediatric populations. Although corticosteroids are effective in alleviating airway diseases, some children with more moderate-severe asthma phenotypes show CS resistance and exhibit significant airflow obstruction, persistent inflammation, and more frequent exacerbations. Previ...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - December 19, 2018 Category: Cytology Authors: Britt RD, Thompson MA, Sasse S, Pabelick CM, Gerber AN, Prakash YS Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Blockade of Enhancer of Zeste Homolog 2 alleviates renal injury associated with hyperuricemia.
In this study, we demonstrated that blockade of EZH2 with 3-DZNeP, a selective EZH2 inhibitor, or silencing of EZH2 with siRNA inhibited uric acid-induced renal fibroblast activation and phosphorylation of Smad3, epidermal growth factor receptor (EGFR) and extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) in cultured renal fibroblasts. Inhibition of EZH2 also suppressed proliferation of renal fibroblasts and epithelial-mesenchymal transition (EMT) of tubular cells. In a mouse model of renal injury induced by hyperuricemia, EZH2 and H3K27me3 expression levels were enhanced, which was coincident with renal dama...
Source: American Journal of Physiology. Renal Physiology - December 19, 2018 Category: Physiology Authors: Shi Y, Xu L, Tao M, Fang L, Lu J, Gu H, Ma S, Lin T, Wang Y, Bao W, Qiu A, Zhuang S, Liu N Tags: Am J Physiol Renal Physiol Source Type: research

An N6-methyladenosine at the transited codon 273 of p53 pre-mRNA promotes the expression of R273H mutant protein and drug resistance of cancer cells.
This study uncovers a novel function of pre-mRNA m6A as a determinant of mutant protein expression in cancer cells heterozygously carrying the TP53 R273H mutation. Suppressing both RNA methylation and ceramide glycosylation might constitute an efficacious and specific approach for targeting TP53 missense mutations coding for a G>A transition, thereby improving cancer treatments. PMID: 30578766 [PubMed - as supplied by publisher]
Source: Biochemical Pharmacology - December 19, 2018 Category: Drugs & Pharmacology Authors: Uddin MB, Roy KR, Hosain SB, Khiste SK, Hill RA, Jois SD, Zhao Y, Tackett AJ, Liu YY Tags: Biochem Pharmacol Source Type: research

Coagulation factor XI induces Ca2+ response and accelerates cell migration in vascular smooth muscle cells via proteinase-activated receptor 1.
In conclusion, FXIa mainly elicits Ca2+ signal via the PAR1/CaV1.2-mediated Ca2+ influx and accelerates the migration in vascular smooth muscle cells. The present study provides the first evidence that FXIa exerts a direct cellular effect on vascular smooth muscle. PMID: 30566391 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - December 19, 2018 Category: Cytology Authors: Liu W, Hashimoto T, Yamashita T, Hirano K Tags: Am J Physiol Cell Physiol Source Type: research

Low-dose mifepristone increased angiogenesis in a manner involving AQP1
ConclusionsLow-dose mifepristone increased angiogenesis in a manner involving AQP1. This affords a new insight into the molecular mechanism underpinning the angiogenic effects of low-dose mifepristone.
Source: Archives of Gynecology and Obstetrics - December 19, 2018 Category: OBGYN Source Type: research

ZNF300 stimulates fatty acid oxidation and alleviates hepatosteatosis through regulating PPAR α.
ZNF300 stimulates fatty acid oxidation and alleviates hepatosteatosis through regulating PPARα. Biochem J. 2018 Dec 19;: Authors: Yan FJ, Wang YJ, Yan SR, Lu J, Zheng YL Abstract ZNF300 plays an important role in the regulation of HBV-related hepatocellular carcinoma. However, little is known about the role of ZNF300 in lipid metabolism and NAFLD. In the present study, we observed that ZNF300 expression was markedly decreased in free fatty acids (FFAs)-induced fatty liver. Overexpressed ZNF300 alleviated hepatic lipid accumulation, whereas knockdown of ZNF300 enhanced the FFAs-induced lipid accumulat...
Source: The Biochemical Journal - December 19, 2018 Category: Biochemistry Authors: Yan FJ, Wang YJ, Yan SR, Lu J, Zheng YL Tags: Biochem J Source Type: research

Downregulation of basic fibroblast growth factor increases cisplatin sensitivity in A549 non-small cell lung cancer cells
Conclusion: bFGF decreased NSCLC sensitivity to cisplatin in vitro, while it enhanced colony formation ability and increased OCT-4 expression of A549 cells, which might account for its involved mechanisms of cisplatin resistance.
Source: Journal of Cancer Research and Therapeutics - December 19, 2018 Category: Cancer & Oncology Authors: Long He Yousheng Meng Zhihui Zhang Yanguo Liu Xiuwen Wang Source Type: research

Transdifferentiation effects and related mechanisms of nerve growth factor and internal limiting membrane on M üller cells.
In this study, we investigated the pro-proliferative, pro-transdifferentiation effects and mechanisms of nerve growth factor (NGF), internal limiting membrane (ILM), and NGF+ILM on Müller cells. The Müller cells cultured with NGF, ILM or both were mediated with tyrosine kinase A (TrkA) (a high affinity receptor for NGF) inhibitor, phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) (an intracellular signaling pathway important in regulating the cell cycle) inhibitor, or LIN28 (a RNA-binding protein and a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cel...
Source: Experimental Eye Research - December 19, 2018 Category: Opthalmology Authors: Zhang L, Li X, Shen Y, Lin X, Wu M Tags: Exp Eye Res Source Type: research

YAP and TAZ are distinct effectors of corneal myofibroblast transformation.
CONCLUSION: Our findings suggest that YAP and TAZ function as distinct modulators of TGFβ1 induced myofibroblast transformation and have different roles in signalling. Specifically, TAZ limits YAP's ability to mediate KFM transformation via Smad proteins. The data also suggest that while having distinct effects, YAP and TAZ have redundant or combinatorial functions critical to cell survival. These results suggest that a loss of TAZ may help drive corneal haze and fibrosis and that the balance between YAP/TAZ is essential in controlling myofibroblast differentiation. PMID: 30578787 [PubMed - as supplied by publisher]
Source: Experimental Eye Research - December 19, 2018 Category: Opthalmology Authors: Muppala S, Raghunathan VK, Jalilian I, Thomasy S, Murphy CJ Tags: Exp Eye Res Source Type: research

Proliferation inhibition and apoptosis promotion by dual silencing of VEGF and Survivin in human osteosarcoma.
Abstract Simultaneous silencing of multiple upregulated genes is an attractive and viable treatment strategy for many incurable diseases including cancer. Herein we used a dual gene-targeted siRNA conjugate composed of VEGF and Survivin siRNA sequences in the same backbone to inhibit proliferation and angiogenesis in two human osteosarcoma cell lines. We synthesized siRNA sequences targeting the VEGF and Survivin genes individually (VEGF siRNA and Survivin siRNA) or simultaneously (one-chain-double-target siRNA: dual siRNA). VEGF and Survivin mRNA and protein expression levels in human osteosarcoma MG-63 and Saos-...
Source: Acta Biochimica et Biophysica Sinica - December 19, 2018 Category: Biochemistry Authors: Gu J, Ji Z, Li D, Dong Q Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

LncRNA GAS5 regulates redox balance and dysregulates the cell cycle and apoptosis in malignant melanoma cells
ConclusionsOur findings show GAS5 contributes to regulation of the apoptosis, cell cycle, homeostasis of reactive oxygen species, and redox balance in MM cells, and suggest that reduced GAS5 expression contributes to disease progression in MM patients.
Source: Journal of Cancer Research and Clinical Oncology - December 19, 2018 Category: Cancer & Oncology Source Type: research

Non-steroidal anti-inflammatory drugs increase MRP4 expression in an endometriotic epithelial cell line in a PPARa dependent manner.
CONCLUSIONS: MRP4 expression was increased in cells treated with NSAIDs and the nuclear receptor PPARa is involved. Elevated PGE2 levels in cell supernatants correlate with its increased transport by MRP4 after NSAID treatment. More importantly, we provide evidence that in endometriotic epithelial cells aspirin and non-aspirin NSAIDs treatments alter gene expression. PMID: 30556891 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - December 18, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

LncRNA SNHG8 promotes the development and chemo-resistance of pancreatic adenocarcinoma.
CONCLUSIONS: SNHG8 is highly expressed in pancreatic adenocarcinoma tissues and is negatively correlated with its prognosis. Moreover, SNHG8 promotes cell proliferation and cell cycle, whereas inhibits cell apoptosis and reduces the chemo-sensitivity of pancreatic adenocarcinoma cells. PMID: 30556854 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - December 18, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

GAS5 promotes podocyte injury in sepsis by inhibiting PTEN expression.
CONCLUSIONS: GAS5 expression decreased in sepsis-induced podocyte injury, and GAS5 was involved in regulating sepsis-induced podocyte injury by reducing PTEN expression. PMID: 30556845 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - December 18, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

The role of TCPTP on leptin effects on astrocyte morphology
Publication date: Available online 17 December 2018Source: Molecular and Cellular EndocrinologyAuthor(s): Lucas Kniess Debarba, Fernanda Maria Veanholi Vechiato, Hellen Veida Paraguassu Silva, Beatriz C. Borges, Maria Célia Jamur, José Antunes-Rodrigues, Lucila Leico Kagohara EliasAbstractLeptin and LPS has been implicated in the development of hypothalamic astrogliosis in rodents. Astrocytes, which are interconnected by gap junction proteins, have emerged as important players in the control of energy homeostasis exerted by the hypothalamus. To investigate the hypothesis of action of T-cell protein tyrosine phosphatase (...
Source: Molecular and Cellular Endocrinology - December 18, 2018 Category: Endocrinology Source Type: research

High Expressions of CUL4A and TP53 in Colorectal Cancer Predict Poor Survival
Conclusion: CUL4A expression correlated positively with the prognosis of colorectal cancer. Mechanistically, ZEB1 was confirmed to mediate the function of CUL4A in regulating the EMT. The assessment of both CUL4A and mutant TP53 expression will be helpful in predicting colon cancer prognosis.Cell Physiol Biochem 2018;51:2829 –2842
Source: Cellular Physiology and Biochemistry - December 18, 2018 Category: Cytology Source Type: research

Intermittent-Hypoxia-Induced Autophagy Activation Through the ER-Stress-Related PERK/eIF2 α/ATF4 Pathway is a Protective Response to Pancreatic β-Cell Apoptosis
Conclusion: IH induces autophagy activation through the ER-stress-related PERK/eIF2 α/ATF4 signaling pathway, which is a protective response to pancreatic β-cell apoptosis caused by IH.Cell Physiol Biochem 2018;51:2955 –2971
Source: Cellular Physiology and Biochemistry - December 18, 2018 Category: Cytology Source Type: research

Achyranthes bidentata polypeptides promotes migration of Schwann cells via NOX4/DUOX2-dependent ROS production in rats
In this study, we aimed to investigate the possible mechanism underlying the ABPP-induced migration of primary cultured rat SCs. Transwell migration assays indicated that ABPP promoted SCs migration in a concentration-dependent manner by inducing production of NADPH-oxidase (NOX)-derived reactive oxygen species (ROS). Inhibition of ROS production by NOXs inhibitor apocynin (APO) or diphenyleneiodonium (DPI) partially blocked ABPP-mediated SCs migration. Furthermore, by using real-time polymerase chain reaction analysis and siRNA interference technique, we verified the participation of NOX subunit 4 (NOX4) and dual oxidase ...
Source: Neuroscience Letters - December 18, 2018 Category: Neuroscience Source Type: research

EIF4A2 interacts with the membrane protein of transmissible gastroenteritis coronavirus and plays a role in virus replication
Publication date: Available online 17 December 2018Source: Research in Veterinary ScienceAuthor(s): Zhenhui Song, Yang Yang, Li Wang, Kai Wang, Ling Ran, Yilu Xie, LeiShi Huang, Zhou Yang, Peng Yuan, Qiuhan YuAbstractTransmissible gastroenteritis coronavirus (TGEV) is enteropathogenic coronavirus that causes diarrhea in pigs, and is associated with high morbidity and mortality in sucking piglets. The TGEV membrane (M) protein is a decisive protein for the proliferation of viral proteins, and is associated with virus assembly and budding. To identify the cellular proteins that interact with the TGEV M protein, yeast two-hyb...
Source: Research in Veterinary Science - December 18, 2018 Category: Veterinary Research Source Type: research

Targeting the lysosome by an aminomethylated Riccardin D triggers DNA damage through cathepsin B-mediated degradation of BRCA1.
This study reveals a novel molecular mechanism that RD-N promotes CTSB-dependent DNA damage by the suppression of BRCA1 in PCa cells, leading to the identification of a potential compound that target lysosomes for cancer treatment. PMID: 30565390 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - December 18, 2018 Category: Molecular Biology Authors: Wang Y, Niu H, Hu Z, Zhu M, Wang L, Han L, Qian L, Tian K, Yuan H, Lou H Tags: J Cell Mol Med Source Type: research

Arsenic modifies serotonin metabolism through glucuronidation in pancreatic β-cells.
Abstract In arsenic-endemic regions of the world, arsenic exposure correlates with diabetes mellitus. Multiple animal models of inorganic arsenic (iAs, As3+) exposure have revealed that iAs-induced glucose intolerance manifests as a result of pancreatic β-cell dysfunction. To define the mechanisms responsible for this β-cell defect, the MIN6-K8 mouse β-cell line was exposed to environmentally-relevant doses of iAs. Exposure to 0.1-1 µM iAs for 3 days significantly decreased glucose-induced insulin secretion (GIIS). Serotonin and its precursor, 5-hydroxytryptophan (5-HTP), were both decreased. Supplementation w...
Source: Am J Physiol Endocri... - December 18, 2018 Category: Endocrinology Authors: Carmean CM, Yokoi N, Takahashi H, Oduori OS, Kang C, Kanagawa A, Kirkley AG, Han G, Landeche M, Hidaka S, Katoh M, Sargis RM, Seino S Tags: Am J Physiol Endocrinol Metab Source Type: research

High calpain-1 expression predicts a poor clinical outcome and contributes to tumor progression in pancreatic cancer patients
ConclusionThe above findings suggest that calpain-1 should be considered a potential biomarker for PC prognosis and therapy.
Source: Clinical and Translational Oncology - December 18, 2018 Category: Cancer & Oncology Source Type: research

Electro-acupuncture-induced neuroprotection is associated with activation of the IGF-1/PI3K/Akt pathway following adjacent dorsal root ganglionectomies in rats.
In conclusion, the results of the present study demonstrated the crucial roles of IGF‑1 in EA‑induced neuroplasticity following adjacent dorsal root ganglionectomies in rats via the PI3K/Akt signaling pathway. PMID: 30569108 [PubMed - as supplied by publisher]
Source: International Journal of Molecular Medicine - December 18, 2018 Category: Molecular Biology Authors: Hu T, Lu MN, Chen B, Tong J, Mao R, Li SS, Dai P, Tan YX, Xiyang YB Tags: Int J Mol Med Source Type: research

Vitamin C Attenuates Sodium Fluoride-Induced Mitochondrial Oxidative Stress and Apoptosis via Sirt1-SOD2 Pathway in F9 Cells.
Abstract Increasing evidence has suggested an important role played by reactive oxygen species (ROS) in the pathogenesis of fluorosis. Accumulating evidence demonstrates that vitamin C administration ameliorate sodium fluoride (NaF)-induced oxidative stress. However, the potentially beneficial effects of vitamin C against NaF-induced cytotoxicity and the underlying molecular mechanisms of this protection are not fully understood. Here, we found that NaF stimulated cytotoxicity, increased mitochondrial reactive oxygen species (mROS) production, and induced apoptosis in F9 embryonic carcinoma cells. Consistent with ...
Source: Biological Trace Element Research - December 18, 2018 Category: Biology Authors: Peng W, Xu S, Zhang J, Zhang Y Tags: Biol Trace Elem Res Source Type: research

A DR4 capturer with AKT siRNA for synergetic enhancement of death receptor-mediated apoptosis
Chem. Commun., 2018, Accepted Manuscript DOI: 10.1039/C8CC06796A, CommunicationWei Pan, Shaohua Gong, Yanhua Li, Huiwen Zhang, Na Li, Bo Tang A graphene-based nanocarrier modified with death receptor 4 (DR4) antibody and AKT siRNA has been developed, which can synergistically strengthen the death receptor-mediated apoptosis and enhance the cancer therapeutic effect... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - November 6, 2018 Category: Chemistry Authors: Wei Pan Source Type: research

Oral siRNA Delivery Using Dual Transporting Systems to Efficiently Treat Colorectal Liver Metastasis
Publication date: Available online 3 November 2018Source: International Journal of PharmaceuticsAuthor(s): Eun-Ju Hyun, Mohammad Nazmul Hasan, Sung Hun Kang, Sungpil Cho, Yong-Kyu LeeAbstractOral siRNA delivery is an ideal way to translate siRNA therapeutic effects in the clinic due to its ability to be administered in convenient and multiple dosages. However, an effective oral delivery system requires overcoming both a hostile gastrointestinal (GI) environment and non-specific targeting. Here, an HTsRP-NC system is a new oral siRNA delivery system consisting of a siRNA/protamine (sRP) nano-complex protected by a multi-fun...
Source: International Journal of Pharmaceutics - November 4, 2018 Category: Drugs & Pharmacology Source Type: research

Combined strategy of siRNA and osteoclast actin cytoskeleton automated imaging to identify novel regulators of bone resorption shows a non-mitotic function for anillin.
Abstract Osteoclasts are the main cells responsible for the resorption of mineralized extracellular matrices. They are the major targets for anti-resorptive therapies to manage osteoporosis, a major public health problem. Osteoclasts are giant multinucleated cells that can organize their a unique adhesion structure based on a belt of podosomes, which is the keystone of the bone resorption apparatus. We combined differential transcriptomics and siRNA screening approaches to get a broader view of cytoskeletal regulators that participate in the control of osteoclast cytoskeleton and identify novel regulators of bone ...
Source: European Journal of Cell Biology - October 31, 2018 Category: Cytology Authors: Maurin J, Morel A, Hassen-Khodja C, Vives V, Jurdic P, Machuca-Gayet I, Blangy A Tags: Eur J Cell Biol Source Type: research

Box Behnken design of siRNA-loaded liposomes for the treatment of a murine model of ocular keratitis caused by Acanthamoeba
In this study, PEGylated cationic liposomes were proposed and optimized using Box-Behnken design. The influence of DOTAP:DOPE ratio, DSPE-PEG concentration, and siRNA/DOTAP charge ratio were evaluated over both biological response and physicochemical properties of liposomes. The ratio of DOTAP:DOPE had an effect in the trophozoite activity whereas the charge ratio influenced both size and protease activity. The predicted values were very close to the observed values, yielding a formulation with good activity and toxicity profile, which was used in the following experiments. A murine model of ocular keratitis was treated wi...
Source: Colloids and Surfaces B: Biointerfaces - October 29, 2018 Category: Biochemistry Source Type: research