Circ_0045714/miR-331-3p interaction affects IL-1 β-evoked human articular chondrocyte injury through regulating PIK3R3 in a ceRNA regulatory cascade
CONCLUSION: There was a novel circ_0045714/miR-331-3p/PIK3R3 ceRNA axis in HAC, and its inhibition might be one mechanism of HAC injury in OA.PMID:34649564 | DOI:10.1186/s13018-021-02738-2 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Ran Ding Jinsong Zhou Jianguo Xu Huajie Lu Tingting Zhang Xiong Xiang Zhen Shi Source Type: research

A retrospective study on prophylactic regional lymphadenectomy versus nodal observation only in the management of dogs with stage I, completely resected, low-grade cutaneous mast cell tumors
CONCLUSIONS: Our results showed that lack of immediate lymphadenectomy was associated with a higher risk for tumor progression. This preliminary judgement, reinforced by the findings that lymphadenectomy was well tolerated in all cases, and that histopathology provides the definitive assessment of the nodal pathological status, may suggest that prophylactic lymphadenectomy is indicated in the management of stage I MCTs. Larger prospective studies are warranted for generating clinical evidence of this latter hypothesis.PMID:34649575 | DOI:10.1186/s12917-021-03043-0 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Silvia Sabattini Matti Kiupel Riccardo Finotello Damiano Stefanello Eugenio Faroni Walter Bertazzolo Ugo Bonfanti Antonella Rigillo Sara Del Magno Armando Foglia Luca Aresu Matteo Gambini Mario Caniatti Laura Marconato Source Type: research

Comparative effectiveness of two methods for inducing osteoarthritis in a novel animal model, the Diannan small-ear pig
CONCLUSIONS: These results indicated that the Diannan small-ear pig can be used as an effective osteoarthritis model. In addition, it is much more convenient and much faster to induce osteoarthritis by intra-articular injection of papain, which is a method worthy of being promoted.PMID:34649596 | DOI:10.1186/s13018-021-02734-6 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Di Jia Ruixian Zhang Yinghong He Guofeng Cai Jiali Zheng Yuye Yang Yanlin Li Source Type: research

Circ_0061140 knockdown inhibits tumorigenesis and improves PTX sensitivity by regulating miR-136/CBX2 axis in ovarian cancer
CONCLUSIONS: Circ_0061140 silencing repressed the progression and PTX resistance of ovarian cancer by downregulating CBX2 expression via sponging miR-136, which provided novel insight into studying the therapy of ovarian cancer with PTX.PMID:34649611 | DOI:10.1186/s13048-021-00888-9 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Jun Zhu Jun-E Luo Yurong Chen Qiong Wu Source Type: research

Minimal residual disease in multiple myeloma: current status
Biomark Res. 2021 Oct 14;9(1):75. doi: 10.1186/s40364-021-00328-2.ABSTRACTMultiple myeloma (MM) is a treatable plasma cell cancer with no cure. Clinical evidence shows that the status of minimal residual disease (MRD) after treatment is an independent prognostic factor of MM. MRD indicates the depth of post-therapeutic remission. In this review article, we outlined the major clinical trials that have determined the prognostic value of MRD in MM. We also reviewed different methods that were used for MM MRD assessment. Most important, we reviewed our current understanding of MM MRD biology. MRD studies strongly indicate that...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Hong Ding Juan Xu Zhimei Lin Jingcao Huang Fangfang Wang Yan Yang Yushan Cui Hongmei Luo Yuhan Gao Xinyu Zhai Weicui Pang Li Zhang Yuhuan Zheng Source Type: research

RBFOX splicing factors contribute to a broad but selective recapitulation of peripheral tissue splicing patterns in the thymus
Genome Res. 2021 Oct 14. doi: 10.1101/gr.275245.121. Online ahead of print.ABSTRACTThymic epithelial cells (TEC) control the selection of a T cell repertoire reactive to pathogens but tolerant of self. This process is known to involve the promiscuous expression of virtually the entire protein-coding gene repertoire, but the extent to which TEC recapitulate peripheral isoforms, and the mechanisms by which they do so, remain largely unknown. We performed the first assembly-based transcriptomic census of transcript structures and splicing factor (SF) expression in mouse medullary TEC (mTEC) and 21 peripheral tissues. Mature m...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Kathrin Jansen Noriko Shikama-Dorn Moustafa Attar Stefano Maio Maria Lopopolo David Buck Georg A Holl änder Stephen N Sansom Source Type: research

Antisense RNAs influence promoter usage of their counterpart sense genes in cancer
In this study, we identified a complete list of differentially expressed ncNATs in hepatocellular carcinoma. Among them, a previously undescribed ncNAT HNF4A-AS1L suppressed cancer cell growth by regulating its sense gene HNF4A, a well-known cancer driver, through a promoter-specific mechanism. HNF4A-AS1L selectively activated the HNF4A P1 promoter via HNF1A, which upregulated expression of tumor suppressor P1-driven isoforms, while having no effect on the oncogenic P2 promoter. RNA-Seq data from 23 tissue and cancer types identified approximately 100 ncNATs whose expression correlated specifically with the activity of one...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Fernando Bellido Molias Andre Sim Ka Wai Leong Omer An Yangyang Song Vanessa Hui En Ng Max Wei Jie Lim Chen Ying Jasmin Xin Jia Teo Jonathan G öke Leilei Chen Source Type: research

Propranolol inhibits the angiogenic capacity of hemangioma endothelia via blocking beta-adrenoceptor in mast cell
CONCLUSIONS: Propranolol inhibit the proliferation of HemECs and promote their apoptosis and autophagy through acting on both β1 and β2 adrenoceptor in mast cell.IMPACT: Treated with propranolol, β1, and β2 adrenoceptor on human mast cell expression was reduced significantly. After hemangioma endothelial cell treated with the supernatants from propranolol-treated human mast cell, its proliferation was decreased, but apoptosis and autophagy were significantly induced. Propranolol can work well in shRNA-ADRB1 or shRNA-ADRB2 transfected HMC-1s. Mast cells may have a role in the action of propranolol in inf...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Ying Ye Huaqing Zhong Limin Dou Wei Song Chenbin Dong Wenmin Lu Kuiran Dong Kai Li Jun Li Lingfeng He Wei Gao Chunmei Xia Liuhui Wang Source Type: research

Low Expression of Stanniocalcin 1 (STC-1) Protein Is Associated With Poor Clinicopathologic Features of Endometrial Cancer
In this study, we investigated the protein expression pattern of STC-1 in a tissue microarray (TMA) cohort of hysterectomy specimens from 832 patients with EC. We then evaluated the prognostic value of STC-1 expression regarding the clinicopathologic features and patients survival over a period of 140 months. Our results revealed that in EC tissue samples, STC-1 is mainly localized in the endometrial epithelium, although some expression was also observed in the stroma. Decreased STC-1 expression was associated with factors relating to a worse prognosis, such as grade 3 endometrioid tumors (p = 0.030), deep myometrial invas...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Masuma Khatun Elina Urpilainen Anne Ahtikoski Riikka K Arffman Annukka Pasanen Ulla Puistola Juha S Tapanainen Leif C Andersson Ralf Butzow Mikko Loukovaara Terhi T Piltonen Source Type: research

Nobiletin, a hexamethoxyflavonoid from citrus pomace, attenuates G1 cell cycle arrest and apoptosis in hypoxia-induced human trophoblast cells of JEG-3 and BeWo via regulating the p53 signaling pathway
CONCLUSION: NOB attenuated hypoxia-induced apoptosis in JEG-3 and BeWo cell lines and might be a potential functional ingredient to prevent pregnancy-related diseases caused by hypoxia-induced apoptosis. These findings would also suggest the exploration and utilization of citrus resources, and the development of citrus industry.PMID:34650395 | PMC:PMC8494266 | DOI:10.29219/fnr.v65.5649 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Mengling Zhang Jian Liu Rui Zhang Zengenni Liang Shenghua Ding Huanling Yu Yang Shan Source Type: research

Metabolic reprogramming in epithelial ovarian cancer
Am J Transl Res. 2021 Sep 15;13(9):9950-9973. eCollection 2021.ABSTRACTCancer cells usually show adaptations to their metabolism that facilitate their growth, invasiveness, and metastasis. Therefore, reprogramming the energy metabolism is one of the current key foci of cancer research and treatment. Although aerobic glycolysis-the Warburg effect-has been thought to be the dominant energy metabolism in cancer, recent data indicate a different possibility, specifically that oxidative phosphorylation (OXPHOS) is the more likely form of energy metabolism in some cancer cells. Due to the heterogeneity of epithelial ovarian canc...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Chalaithorn Nantasupha Chanisa Thonusin Kittipat Charoenkwan Siriporn Chattipakorn Nipon Chattipakorn Source Type: research

LncRNA-MIAT regulates the growth of SHSY5Y cells by regulating the miR-34-5p-SYT1 axis and exerts a neuroprotective effect in a mouse model of Parkinson's disease
Am J Transl Res. 2021 Sep 15;13(9):9993-10013. eCollection 2021.ABSTRACTTo examine the neuroprotective roles of lncRNA-MIAT in Parkinson's disease (PD). RNA sequencing expression profiles were utilized to screen the dysregulated lncRNAs in patients with PD and to explore the underlying molecular mechanisms by which the lncRNAs regulate the pathogenesis of PD. 6-hydroxydopamine-induced SH-SY5Y cell lines and a PD mouse model were used to prove how the overexpressing or knocking-down of MIAT produce a marked effect in both in vitro and in vivo experiments. Subsequently, the subcellular localization of MIAT was detected via R...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Yue'e Shen Xintao Cui Yuhang Hu Zhizhuang Zhang Zhenyu Zhang Source Type: research

ZLN005 protects against ischemia-reperfusion-induced kidney injury by mitigating oxidative stress through the restoration of mitochondrial fatty acid oxidation
Am J Transl Res. 2021 Sep 15;13(9):10014-10037. eCollection 2021.ABSTRACTTo date, the treatment of acute kidney injury (AKI) remains a difficult problem for clinicians. In the present study, we assessed whether ZLN005, a novel peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) agonist, can protect against ischemic AKI in vivo and in vitro. Notably, ZLN005 treatment significantly alleviated Ischemia-reperfusion (I/R)-induced tubular injury and reversed the decrease in hypoxia-reoxygenation-induced cell viability by restoring PGC-1α expression in a dose-dependent manner. This benefic...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Zhiyu Wang Zongjie Fu Chongjian Wang Jing Xu Hongkun Ma Mengdi Jiang Tingting Xu Xiaobei Feng Wen Zhang Source Type: research

CircRNA_002178 as a ceRNA promotes the development of colorectal cancer by regulating miR-542-3p/CREB1
This study aimed to explore the effect of circRNA_002178 as a competing endogenous RNA in the development of CRC by regulating the miR-542-3p/cAMP response element binding protein 1 (CREB1) axis and its molecular mechanism.METHODS: The relative expressions of circ_002178, miR-542-3p, and CREB1 in patients' cell lines and CRC tissues were measured using Western blot and qRT-PCR. The localization and expression of circ_002178 were determined using fluorescence in situ hybridization and nucleocytoplasmic separation tests. The targeting relationships among circ_002178, miR-542-3p, and CREB1 were validated using RNA immunopreci...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Ying Xu Chunliang Wang Xingang Lu Ruihua Qi Xiao Wang Jinrong Zhao Source Type: research

HNSC exosome-derived MIAT improves cognitive disorders in rats with vascular dementia < em > via < /em > the miR-34b-5p/CALB1 axis
Am J Transl Res. 2021 Sep 15;13(9):10075-10093. eCollection 2021.ABSTRACTOBJECTIVE: To explore the molecular mechanism by which hippocampal neural stem cell (HNSC) exosome (exo)-derived MIAT improves cognitive disorders in rats with vascular dementia (VD).METHODS: Rat hippocampal tissues were collected, and HNSCs and hippocampal neuronal cells (HNCs) were isolated, purified, and identified. Then the exosomes (exo) of the HNSCs were extracted and identified. A VD rat model was constructed. HE staining was used to evaluate the hippocampal pathology in each group. The expressions of the RNAs in the HNSCs were intervened, and ...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Dan Qi Xiaojun Hou Chunfeng Jin Xi Chen Chengli Pan Hongjuan Fu Leifeng Song Jujun Xue Source Type: research

Carcinoma-associated fibroblasts promote the proliferation and metastasis of osteosarcoma by transferring exosomal LncRNA SNHG17
Am J Transl Res. 2021 Sep 15;13(9):10094-10111. eCollection 2021.ABSTRACTCancer-associated fibroblasts (CAFs) serve as a predominant regulator in the tumor microenvironment. However, the crosstalk between CAFs and OS cells remains mostly unclear. Recent studies explored that long non-coding RNA (LncRNAs) involved in regulating osteosarcoma (OS) formation and development, but their functions in CAFs are unknown. Here, we first investigated the SNHG17 was upregulated in OS tissues and correlated with the poor prognosis through the integrating clinical data. We then evaluated the function of SNHG17 in vitro using the stable S...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Aiqing Zhao Zhenqun Zhao Wanlin Liu Xiaolong Cui Na Wang Yong Wang Yuxin Wang Liang Sun Huiqin Xue Lishuan Wu Shuxia Cui Yun Yang Rui Bai Source Type: research

CircMYC regulates the mitochondrial respiration and cell viability via miR-516a-5p/AKT3 axis in acute myeloid leukemia
This study aimed at exploring the regulatory function of circMYC in AML progression.METHODS: Expression levels of CircMYC, miR-516a-5p, AKT3 and apoptosis-related proteins were determined by RT-qPCR and western blot. Cell viability and proliferation were examined by CCK8 assay and EdU assay. Annexin V/PI staining was used to assess cell apoptosis. Mitochondrial respiration function was confirmed by oxygen consumption rate (OCR). The relationships among circMYC, miR-516a-5p and AKT3 were detected by dual-luciferase reporter (DLR) assay, RNA-pull down assay and RNA immunoprecipitation (RIP) assay, respectively.RESULTS: CircM...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Xueqin Zou Min Jiang Source Type: research

Coenzyme Q < sub > 10 < /sub > amends testicular function and spermatogenesis in male mice exposed to cigarette smoke by modulating oxidative stress and inflammation
This study explored the effects of coenzyme Q10 (CoQ10) on the testicular functions of male mice exposed to cigarette smoke. Eight-week-old BALB/c male mice were divided into the following groups: the AV group (air with a vehicle), the AQ group (air with CoQ10), the SV group (smoke with a vehicle), and the SQ group (smoke with CoQ10). The results showed that the CoQ10 concentrations in the sera and testes were decreased in the groups subjected to smoke but they were improved after the administration of CoQ10. Neither smoke nor CoQ10 supplementation affected the serum or testis testosterone concentrations. Regarding the ant...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: You-Shuei Lin Chin-Yu Liu Pei-Wen Chen Cheng-Yi Wang Hsin-Chih Chen Chih-Wei Tsao Source Type: research

The correlation of the miR-29a/MMP9 axis with < em > Helicobacter pylori < /em > infection in gastric cancer
This study was designed to investigate the association between the miR-29a/MMP9 axis expression levels and Helicobacter pylori (HP) infection in gastric cancer patients.METHODS: A total of 100 gastric cancer patients referred to our hospital from June 2017 to June 2019 were recruited as the study cohort. Among them, 50 HP-positive patients were included in the experimental group and 50 HP-negative patients were included in the control group. The changes in the patients' conditions were compared, the miR-29a/MMP9 axis expression levels were recorded, and the correlation between the miR-29a/MMP9 axis and the HP infections wa...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Lunqin Liu Yuecui Li Xia Zhang Hong Zhang Source Type: research

Identification of FAM107A as a potential biomarker and therapeutic target for prostate carcinoma
Am J Transl Res. 2021 Sep 15;13(9):10163-10177. eCollection 2021.ABSTRACTFAM107A may have a dual role in regulating the biological functions of tumors; however, its role in prostate adenocarcinoma (PRAD) remains unknown. We analyzed FAM107A expression by employing databases to clarify its potential prognostic value for PRAD, as well as its role in the pathogenesis of PRAD. We observed that the FAM107A expression level is decreased in PRAD, and the reduced expression is considerably associated with poor overall survival and progression-free survival (PFS). To explore the mechanism of FAN107A in PRAD, we performed an immune ...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Yun-Fei Ma Guang-Da Li Xu Sun Xiao-Xiao Li Yu Gao Chong Gao Ke-Xin Cao Guo-Wang Yang Ming-Wei Yu Xiao-Min Wang Source Type: research

The exosomal transfer of human bone marrow mesenchymal stem cell-derived miR-1913 inhibits osteosarcoma progression by targeting NRSN2
CONCLUSION: The inhibitory role of hBMSC-derived miR-1913 on osteosarcoma progression was achieved by targeting NRSN2, indicating the potential therapeutic value of hBMSC-derived miR-1913.PMID:34650689 | PMC:PMC8507079 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Jihui Zhou Lili Xu Peng Yang Yao Lu Shibang Lin Guanghai Yuan Source Type: research

1-n-heptyl-5-(3, 4-difluorophenyl) biguanide inhibits non-small cell lung cancer cell growth by downregulating the EGFR signaling pathways
Am J Transl Res. 2021 Sep 15;13(9):10193-10205. eCollection 2021.ABSTRACTLung cancer is among the diseases with the highest rates of morbidity and mortality. Our previous study found that a novel biguanide derivative, 1-n-heptyl-5-(3, 4-difluorophenyl) biguanide (8e) shows excellent anti-proliferative activity in non-small cell lung cancer (NSCLC) cell line A549. However, the underlying mechanism remains elusive. In this research, we analyzed the effect of 8e on NSCLC cell lines and explored the cell death mechanism caused by 8e. From our data, we found that 8e significantly decreased the cell activity and inhibited the co...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Di Xiao Mei Peng Yang Deng Yayi Xiao Xinyi Chen Lan Ma Bei Tang Chenglin Guo Xiaoping Yang Source Type: research

CDC45 modulates MCM7 expression and inhibits cell proliferation by suppressing the PI3K/AKT pathway in acute myeloid leukemia
This study aimed to identify new targets for the diagnosis and treatment of AML. The GSE65409 and GSE90062 were selected from the AML database of the Gene Expression Omnibus and compared using the GEO2R tool to identify differentially expressed genes (DEGs). The Database for Annotation, Visualization, and Integrated Discovery was used to perform gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses of the DEGs. Protein-protein interactions were visualized using the Search Tool for the Retrieval of Interacting Genes, which identified two potential hub genes that encode CDC45 and MCM7. Relative to AML specimens,...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Rong Zhang Zhuogang Liu Guojun Zhang Source Type: research

LncRNA MALAT1 promotes breast cancer progression by sponging miR101-3p to mediate mTOR/PKM2 signal transmission
This study examined the role and underlying mechanisms of long non-coding RNA (lncRNA), metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in BC pathogenesis. The MALAT1 expression levels in BC cells and tissues were measured using quantitative reverse transcription-polymerase chain reactions. CCK-8 kits and wound healing and transwell assays were used to evaluate the cell growth, invasion, and migration of BC. Bioinformatics and dual-luciferase reporter analyses were conducted to identify MALAT1's potential targets. The protein levels in the mTOR/PKM2 pathway were assessed using Western blot analyses. The MAL...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Jianqiang Shao Qin Zhang Peng Wang Zunyi Wang Source Type: research

ITIH5 shows tumor suppressive properties in cervical cancer cells grown as multicellular tumor spheroids
Am J Transl Res. 2021 Sep 15;13(9):10298-10314. eCollection 2021.ABSTRACTCervical cancer (CC) arises from premalignant cervical intraepithelial neoplasia (CIN) induced by a persistent infection with human papillomaviruses. The multi-stepwise disease progression is driven by genetic and epigenetic alterations. Our previous studies demonstrated a clear downregulation of inter-α-trypsin-inhibitor-heavy chain 5 (ITIH5) at mRNA and protein levels in CC compared to CIN2/3 and normal cervical tissue. Initial in vitro functional analyses revealed a suppressive effect of ITIH5 on relevant mechanisms for cancer progression in ...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Ann-Kathrin Daum Jessica Dittmann Lars Jansen Sven Peters Uta Dahmen Julia I Heger Felix Hoppe-Seyler Alexandra Gille Joachim H Clement Ingo B Runnebaum Matthias D ürst Claudia Backsch Source Type: research

Fraxetin attenuates ferroptosis in myocardial infarction via AKT/Nrf2/HO-1 signaling
CONCLUSION: Fra attenuated MI-induced ferroptosis via AKT/Nrf2/HO-1 signaling, providing a potential therapeutic agent for MI.PMID:34650699 | PMC:PMC8507044 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Yifei Xu Haiyan Lin Huan Wang Jie Pang Ying Zhou Source Type: research

Knockdown of TRIM11 suppresses cell progression and apoptosis of cervical cancer cells via PI3K/AKT pathway
CONCLUSION: Hence, TRIM11 regulates CC cell progression by modulating PI3K/AKT pathway. The results suggested that TRIM11 might be a possible target for the diagnosis and prognosis of CC patients.PMID:34650700 | PMC:PMC8507035 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Wenfeng Liu Zuoli Wu Lijuan Wang Qijun Wang Xiuhua Sun Shufeng Niu Source Type: research

miR-145-5p inhibits psoriasis progression by regulating the Wnt/ β-catenin pathway
Am J Transl Res. 2021 Sep 15;13(9):10439-10448. eCollection 2021.ABSTRACTOBJECTIVE: This research aimed to probe into the effect of miR-145-5p in psoriasis by regulating Wnt/β-catenin.METHODS: A total of 45 psoriasis patients treated in our hospital were enrolled into an observation group (OG), and other 40 healthy individuals in physical examination over the same period were enrolled into a control group (CG). miR-145-5p in both groups was quantified, and its value in diagnosis and recurrence prediction of psoriasis was analyzed. Additionally, miR-145-5p was transfected into HaCat cells to analyze the biological beha...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Yihan Wang Yi Cao Source Type: research

Effect of levetiracetam in combination with topiramate on immune function, cognitive function, and neuronal nutritional status of children with intractable epilepsy
CONCLUSIONS: Levetiracetam in combination with topiramate is associated with reduced inflammatory response and improved immune function, cognitive function, and neuronal nutritional status in children with intractable epilepsy.PMID:34650715 | PMC:PMC8506992 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Danfeng Pan Xiaoxiao Li Jinbiao Jiang Lingling Luo Source Type: research

Analysis of polymorphonuclear cell apoptosis and inflammatory cytokines in trauma patients in the emergency department
CONCLUSION: Findings of laboratory tests targeting trauma patients who required hospitalization showed that levels of inflammatory cytokines such as TNF-α were increased in ICU-hospitalized patients. PMN cell apoptosis was reduced according to the initial laboratory data of patients with hemorrhagic shock in the emergency department.PMID:34650734 | PMC:PMC8507036 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Sung-Hyuk Choi Jung-Youn Kim Young-Hoon Yoon Source Type: research

Nursing countermeasures for VSD treatment of orthopedic trauma and infected wounds
This study intended to investigate the effect of vacuum sealing drainage (VSD) in the treatment of orthopedic trauma and its role in the clinical care.METHODS: A total of 104 patients with orthopedic trauma infection admitted to our hospital from January 2019 to January 2020 were divided into control group (n=53, receiving VSD) and study group (n=51, receiving VSD and nursing interventions) by random number table. Surgical outcomes, satisfaction, size of trauma and visual analogue scoring scale (VAS) scores were compared between the two groups.RESULTS: The study group had shorter time to achieve granulation tissue coverage...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Xin Xu Yanhua Sun Source Type: research

Applied research on confocal microscopy through focusing detection of tarsal glands shape following phacoemulsification for cataract
CONCLUSION: IVCM plays a vital role in the early diagnosis, in the severity grading, and in the evaluation of the clinical effectiveness of MGD-related diseases, by which the morphological changes of the tarsal gland after phacoemulsification can be observed in a timely manner to predict the occurrence of MGD.PMID:34650750 | PMC:PMC8507046 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Mengke Yuan Qin Zhang Libin Chang Source Type: research

LncRNA GAS5 enhances radiosensitivity of hepatocellular carcinoma and restricts tumor growth and metastasis by miR-144-5p/ATF2
CONCLUSION: Overexpression of GAS5 upregulates ATF2 through miR-144-5p and is able to enhance the radiosensitivity of HCC.PMID:34650771 | PMC:PMC8506991 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Chuanyun Yu Yi Liang Yiqiang Jin Qinghuan Li Source Type: research

Baicalin promotes chondrocyte viability and the synthesis of extracellular matrix through TGF- β/Smad3 pathway in chondrocytes
CONCLUSION: BA promotes chondrocyte viability and the cell matrix synthesis through TGF-β/Smad3 pathway in IL-1β-treated chondrocytes and DMM treated mice. BA is a potential therapeutic target for OA.PMID:34650772 | PMC:PMC8507001 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Pengzhen Wang Jian Liu Shaoheng Zhang Pingping Zhu Xifeng Xiong Chaosheng Yu Aiguo Li Zhihe Liu Source Type: research

A crucial role for the long non-coding RNA < em > CASC11 < /em > in the pathogenesis of human cancers
Am J Transl Res. 2021 Sep 15;13(9):10922-10932. eCollection 2021.ABSTRACTLong non-coding RNAs (lncRNAs) are non-coding RNAs more than 200 nucleotides in length. Although they do not encode proteins, lncRNAs can regulate gene expression at the transcriptional, post-transcriptional, and epigenetic levels. Emerging data show that lncRNAs are important for tumorigenesis and cancer progression. Cancer susceptibility candidate 11 (CASC11) is a prominent lncRNA that is upregulated in various types of cancers. Moreover, its overexpression correlates with larger tumor size, more advanced cancer stages, cancer metastasis, and poor o...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Lian Zheng Zhenjie Guan Miaomiao Xue Source Type: research

The angiostatic peptide endostatin enhances mortality risk prediction in pulmonary arterial hypertension
ERJ Open Res. 2021 Oct 11;7(4):00378-2021. doi: 10.1183/23120541.00378-2021. eCollection 2021 Oct.ABSTRACTCurrently available noninvasive markers for assessing disease severity and mortality risk in pulmonary arterial hypertension (PAH) are unrelated to fundamental disease biology. Endostatin, an angiostatic peptide known to inhibit pulmonary artery endothelial cell migration, proliferation and survival in vitro, has been linked to adverse haemodynamics and shortened survival in small PAH cohorts. This observational cohort study sought to assess: 1) the prognostic performance of circulating endostatin levels in a large, mu...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Catherine E Simpson Megan Griffiths Jun Yang Melanie K Nies R Dhananjay Vaidya Stephanie Brandal Lisa J Martin Michael W Pauciulo Katie A Lutz Anna W Coleman Eric D Austin D Dunbar Ivy William C Nichols Allen D Everett Paul M Hassoun Rachel L Damico Source Type: research

B7-H4 Expression in Precancerous Lesions of the Uterine Cervix
Biomed Res Int. 2021 Oct 5;2021:5857092. doi: 10.1155/2021/5857092. eCollection 2021.ABSTRACTOver 10% of patients diagnosed with cervical intraepithelial neoplasia (CIN) have no lesions detected in their cervical conization specimens. The purpose of this study was to determine the factors related to the absence of such lesions. We particularly sought to investigate whether the expression of B7-H4 in precancerous lesions and cancer of the uterine cervix plays a role in the presence or absence of residual lesions in conization specimens and whether this protein is associated with T cells (i.e., Foxp3+ regulatory T cells, CD4...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Qianqian Zhang Liju Zong Hui Zhang Wei Xie Fan Yang Wenwen Sun Baoxia Cui Youzhong Zhang Source Type: research

A Bioinformatics Approach for the Prediction of Immunogenic Properties and Structure of the SARS-COV-2 B.1.617.1 Variant Spike Protein
CONCLUSION: Together, the in silico analysis revealed the predicted structure of partial S protein, immunogenic properties, and possible regions for S protein of SARS-CoV-2 and provides a valuable prelude for engineering the targeted vaccine or drug against B.1.617.1 variant of SARS-CoV-2.PMID:34651048 | PMC:PMC8510791 | DOI:10.1155/2021/7251119 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Vijay Kumar Srivastava Sanket Kaushik Gazal Bhargava Ajay Jain Juhi Saxena Anupam Jyoti Source Type: research

Gene Expression Characteristics of Liver Tissue Reveal the Underlying Pathogenesis of Hepatocellular Carcinoma
CONCLUSIONS: Based on a series of investigations, it was found that miR-30b-5p may participate in the peroxisome signal transduction by downregulating ABCD3-mediated module 1, thereby promoting the development and progression of hepatocellular carcinoma. Our research results not only provide a theoretical basis for biologists to study hepatocellular carcinoma further but also offer new methods and new ideas for the personalized care and treatment of hepatocellular carcinoma.PMID:34651050 | PMC:PMC8506137 | DOI:10.1155/2021/9458328 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Congfang Guo Xiang Guo Yudong Rong Yirui Guo Li Zhang Source Type: research

Effects of remote ischemic preconditioning (RIPC) and chronic remote ischemic preconditioning (cRIPC) on levels of plasma cytokines, cell surface characteristics of monocytes and in-vitro angiogenesis: a pilot study
Basic Res Cardiol. 2021 Oct 14;116(1):60. doi: 10.1007/s00395-021-00901-8.ABSTRACTRemote ischemic preconditioning (RIPC) protects the heart against myocardial ischemia/reperfusion (I/R) injury and recent work also suggested chronic remote ischemic conditioning (cRIPC) for cardiovascular protection. Based on current knowledge that systemic immunomodulatory effects of RIPC and the anti-inflammatory capacity of monocytes might be involved in cardiovascular protection, the aim of our study was to evaluate whether RIPC/cRIPC blood plasma is able to induce in-vitro angiogenesis, identify responsible factors and evaluate the effe...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Lars Hummitzsch Karina Zitta Lena Fritze Jonas Monnens Patrick Vollertsen Matthias Lindner Rene Rusch Katharina Hess Matthias Gruenewald Markus Steinfath Fred F ändrich Rouven Berndt Martin Albrecht Source Type: research

Combined biological effects of silver nanoparticles and heavy metals in different target cell lines
Environ Sci Pollut Res Int. 2021 Oct 14. doi: 10.1007/s11356-021-16395-9. Online ahead of print.ABSTRACTSilver nanoparticles (AgNPs) and heavy metals are considered to coexist in the environment. Increasing evidence shows that AgNPs can interact with heavy metals; however, the impact of distinct exposure conditions on their combined toxicity is still largely unknown. Here, we investigated the co-effects of AgNPs and heavy metals, including arsenic (As), cadmium (Cd), and nickel (Ni), in target cell lines. The results demonstrated that pretreated with polyvinylpyrrolidone-coated (PVP-coated) AgNPs at noncytotoxic concentrat...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Xue Wang An Xu Yun Liu Source Type: research

Biological aspects of the nasal turbinates of the Anatolian shepherd dog captured from Egypt: Using computed tomography, histological, and scanning electron microscopic observations
Microsc Res Tech. 2021 Oct 15. doi: 10.1002/jemt.23962. Online ahead of print.ABSTRACTThe current study was designed to describe the nasal turbinates of 15 heads of Anatolian shepherd dogs using the histology and scanning electron microscope. The caudal part of the nasal cavity is almost occupied by the ethmoidal concha that is related to the high dog's smelling. Keratinized stratified squamous epithelial lining of the rostral part of dorsal and ventral concha were interdigitated with the underlying lamina propria, with numerous sebaceous and sweat glands. The pseudostratified squamous epithelium lining of the middle part ...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Mohamed M A Abumandour Raafat El-Bakary El-Said Enany Ashraf Karkoura Sara Farid Source Type: research

Similarities and differences between multiple sclerosis and type 1 diabetes
This article is protected by copyright. All rights reserved.PMID:34651395 | DOI:10.1002/dmrr.3505 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Valeria Pozzilli Eleonora Agata Grasso Valentina Tomassini Source Type: research

Enhanced neural differentiation of human-induced pluripotent stem cells on aligned laminin-functionalized polyethersulfone nanofibers; a comparison between aligned and random fibers on neurogenesis
J Biomed Mater Res A. 2021 Oct 14. doi: 10.1002/jbm.a.37320. Online ahead of print.ABSTRACTDespite the numerous attempts in nerve tissue engineering, no ideal strategy has been translated into effective therapy for neuronal regeneration yet. Here, we designed a novel nerve regeneration scaffold combining aligned laminin-immobilized polyethersulfone (PES) nanofibers and human-induced pluripotent stem cells (hiPSCs) for transplantation strategies. Aligned and random PES nanofibers were fabricated by electrospinning method with a diameter of 95-500 nm and were then modified with covalent laminin bounding subsequent to O2 plas...
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Marzieh Ghollasi Delaram Poormoghadam Source Type: research

Autism severity aggregates with family psychiatric history in a community-based autism sample
This study explored how family psychiatric history is related to clinical presentation of Autism Spectrum Disorder (ASD). Higher amounts of first-degree family psychiatric history was associated with higher autism symptom severity as measured by the Social Responsiveness Scale, Second Edition (SRS-2). The contribution of psychiatric family history requires ongoing investigation.PMID:34652072 | DOI:10.1002/aur.2625 (Source: Cell Research)
Source: Cell Research - October 15, 2021 Category: Cytology Authors: Danielle Sipsock Hasmik Tokadjian Giulia Righi Eric M Morrow Stephen J Sheinkopf Rhode Island Consortium for Autism Research and Treatment (RI-CART) Source Type: research

CiRS-7/CDR1as; An oncogenic circular RNA as a potential cancer biomarker
Pathol Res Pract. 2021 Sep 29;227:153639. doi: 10.1016/j.prp.2021.153639. Online ahead of print.ABSTRACTCircular RNAs (circRNAs) as a new class of non-coding RNAs (ncRNAs) play role in gene regulation in multicellular organisms via various interactions with nucleic acids, proteins and particularly microRNAs. They have been found to be involved in a number of biological functions particularly in regulation of cell cycle, and extracellular interactions. Thus, dysregulation of circRNAs is found to be associated with several human diseases and especially numerous types of cancers. ciRS-7 is an example of circRNAs which have be...
Source: Cell Research - October 14, 2021 Category: Cytology Authors: Yazdan Rahmati Yahya Asemani Shahin Aghamiri Fatemeh Ezzatifar Sajad Najafi Source Type: research

Posttraumatic natural killer cell decrease is associated with septic complications
CONCLUSIONS: Our data support the association of trauma-induced NK cell decrease with a subsequent significantly higher rate of septic and surgical complications and suggest that these immune cells might play an important role in antibacterial immunity. Strengthening the NK cell function or limiting their decrease in the postoperative course might be of therapeutic value in severely injured trauma patients.PMID:34649071 | DOI:10.1016/j.jss.2021.08.036 (Source: Cell Research)
Source: Cell Research - October 14, 2021 Category: Cytology Authors: Martin Mauser Deirdr é Kruger Sugeshnee Pather Frank Plani Source Type: research

Generation of induced pluripotent stem cell lines from peripheral blood mononuclear cells of three drug resistant and three drug responsive epilepsy patients
Stem Cell Res. 2021 Oct 9;56:102564. doi: 10.1016/j.scr.2021.102564. Online ahead of print.ABSTRACTEpilepsy is a common neurological disorder characterized by seizures. Unfortunately, 30-40% of all epilepsy patients are resistant to at least two or more anti-seizure medications. Attempts to treat these patients and prevent further seizures necessitates multiple drug trials for the patient. Here we describe the generation and validation of induced pluripotent stem cell (iPSC) lines from peripheral blood mononuclear cells (PBMCs) from 3 drug responsive and 3 drug resistant patients, using a non-integrative Sendai virus vecto...
Source: Cell Research - October 14, 2021 Category: Cytology Authors: Zoe L Hunter Hannah C Leeson Mohammed R Shaker Ernst J Wolvetang Lata Vadlamudi Source Type: research

Telomerase-Positive Somatic Tissues of Honeybee Queens (Apis mellifera) Display No DNA Replication
We report that the somatic tissues of honeybee queens (Apis mellifera) are associated with upregulated telomerase activity; however, this upregulation does not fully correlate with the rate of DNA replication in the tissues. This indicates a noncanonical role of telomerase in the somatic tissues of honeybee queens.PMID:34649236 | DOI:10.1159/000518888 (Source: Cell Research)
Source: Cell Research - October 14, 2021 Category: Cytology Authors: Justina Koubov á Radmila Čapková Frydrychová Source Type: research

Circ_ROBO2/miR-149 Axis Promotes the Proliferation and Migration of Human Aortic Smooth Muscle Cells by Activating NF- κB Signaling
Cytogenet Genome Res. 2021 Oct 14:1-11. doi: 10.1159/000517294. Online ahead of print.ABSTRACTAtherosclerosis is the leading global cause of mortality. The occurrence of coronary artery disease (CAD) is regulated by a diversity of pathways, including circRNAs. However, the potential mechanisms of circRNAs in CAD remain unclear. Here, qRT-PCR was used to examine the expressions of miR-149 and circ_ROBO2. Their influences on cell proliferation, migration, and apoptosis were measured by CCK-8, trans-well, and flow cytometry assays, respectively. The protein levels of p-IκBα and NF-κB p65 were examined using ...
Source: Cell Research - October 14, 2021 Category: Cytology Authors: Dong-Sheng Lin Chi-Yuan Zhang Liang Li Guo-Hong Ye Lu-Ping Jiang Qiao Jin Source Type: research