Mesenchymal stromal cell therapy for damaged retinal ganglion cells, is gold all that glitters?
idal-Sanz M, Agudo-Barriuso M Abstract Mesenchymal stromal cells are an excellent source of stem cells because they are isolated from adult tissues or perinatal derivatives, avoiding the ethical concerns that encumber embryonic stem cells. In preclinical models, it has been shown that mesenchymal stromal cells have neuroprotective and immunomodulatory properties, both of which are ideal for central nervous system treatment and repair. Here we will review the current literature on mesenchymal stromal cells, focusing on bone marrow mesenchymal stromal cells, adipose-derived mesenchymal stromal cells and mesenchymal ...
Source: Cell Research - July 12, 2019 Category: Cytology Authors: Lucas-Ruiz F, Galindo-Romero C, García-Bernal D, Norte-Muñoz M, Rodríguez-Ramírez KT, Salinas-Navarro M, Millán-Rivero JE, Vidal-Sanz M, Agudo-Barriuso M Tags: Neural Regen Res Source Type: research

Diabetic neuropathy research: from mouse models to targets for treatment.
Abstract Diabetic neuropathy is one of the most serious complications of diabetes, and its increase shows no sign of stopping. Furthermore, current clinical treatments do not yet approach the best effectiveness. Thus, the development of better strategies for treating diabetic neuropathy is an urgent matter. In this review, we first discuss the advantages and disadvantages of some major mouse models of diabetic neuropathy and then address the targets for mechanism-based treatment that have been studied. We also introduce our studies on each part. Using stem cells as a source of neurotrophic factors to target extrin...
Source: Cell Research - July 12, 2019 Category: Cytology Authors: Pham VM, Matsumura S, Katano T, Funatsu N, Ito S Tags: Neural Regen Res Source Type: research

The role of muscle LIM protein in the nervous system.
PMID: 31290445 [PubMed] (Source: Cell Research)
Source: Cell Research - July 12, 2019 Category: Cytology Authors: Terheyden-Keighley D, Fischer D Tags: Neural Regen Res Source Type: research

G protein-coupled receptor 37 (GPR37) emerges as an important modulator of adenosinergic transmission in the striatum.
PMID: 31290447 [PubMed] (Source: Cell Research)
Source: Cell Research - July 12, 2019 Category: Cytology Authors: Morato X, Cunha RA, Ciruela F Tags: Neural Regen Res Source Type: research

ELF-1 expression in nasopharyngeal carcinoma facilitates proliferation and metastasis of cancer cells via modulation of CCL2/CCR2 signaling.
Conclusion: Our data suggest that ELF-1 plays an oncogenic role in NPC development associated with the CCL2/CCR2 signaling pathway and may therefore be a potential target for NPC therapy. PMID: 31289447 [PubMed] (Source: Cell Research)
Source: Cell Research - July 12, 2019 Category: Cytology Authors: Chen CH, Su LJ, Tsai HT, Hwang CF Tags: Cancer Manag Res Source Type: research

Contralateral C7 transfer combined with acellular nerve allografts seeded with differentiated adipose stem cells for repairing upper brachial plexus injury in rats.
This study was approved by the Animal Ethics Committee of the First Affiliated Hospital of Sun Yat-sen University of China (approval No. 2016-150) in June 2016. PMID: 31290451 [PubMed] (Source: Cell Research)
Source: Cell Research - July 12, 2019 Category: Cytology Authors: Yang JT, Fang JT, Li L, Chen G, Qin BG, Gu LQ Tags: Neural Regen Res Source Type: research

Hyodeoxycholic acid protects the neurovascular unit against oxygen-glucose deprivation and reoxygenation-induced injury in vitro.
This study was approved by the Ethics Committee of Experimental Animals of Beijing University of Chinese Medicine (approval No. BUCM-3-2016040201-2003) in April 2016. PMID: 31290452 [PubMed] (Source: Cell Research)
Source: Cell Research - July 12, 2019 Category: Cytology Authors: Li CX, Wang XQ, Cheng FF, Yan X, Luo J, Wang QG Tags: Neural Regen Res Source Type: research

The next-generation sphingosine-1 receptor modulator BAF312 (siponimod) improves cortical network functionality in focal autoimmune encephalomyelitis.
euth SG Abstract Autoimmune diseases of the central nervous system (CNS) like multiple sclerosis (MS) are characterized by inflammation and demyelinated lesions in white and grey matter regions. While inflammation is present at all stages of MS, it is more pronounced in the relapsing forms of the disease, whereas progressive MS (PMS) shows significant neuroaxonal damage and grey and white matter atrophy. Hence, disease-modifying treatments beneficial in patients with relapsing MS have limited success in PMS. BAF312 (siponimod) is a novel sphingosine-1-phosphate receptor modulator shown to delay progression in PMS....
Source: Cell Research - July 12, 2019 Category: Cytology Authors: Hundehege P, Cerina M, Eichler S, Thomas C, Herrmann AM, Göbel K, Müntefering T, Fernandez-Orth J, Bock S, Narayanan V, Budde T, Speckmann EJ, Wiendl H, Schubart A, Ruck T, Meuth SG Tags: Neural Regen Res Source Type: research

Silencing Huwe1 reduces apoptosis of cortical neurons exposed to oxygen-glucose deprivation and reperfusion.
Abstract HECT, UBA and WWE domain-containing 1 (Huwe1), an E3 ubiquitin ligase involved in the ubiquitin-proteasome system, is widely expressed in brain tissue. Huwe1 is involved in the turnover of numerous substrates, including p53, Mcl-1, Cdc6 and N-myc, thereby playing a critical role in apoptosis and neurogenesis. However, the role of Huwe1 in brain ischemia and reperfusion injury remains unclear. Therefore, in this study, we investigated the role of Huwe1 in an in vitro model of ischemia and reperfusion injury. At 3 days in vitro, primary cortical neurons were transduced with a control or shRNA-Huwe1 lentivir...
Source: Cell Research - July 12, 2019 Category: Cytology Authors: He GQ, Xu WM, Liao HJ, Jiang C, Li CQ, Zhang W Tags: Neural Regen Res Source Type: research

Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats.
Abstract Neuropathic pain after spinal cord injury (SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy; nevertheless, the ideal cell type in terms of neurogenic potential and effectiveness against pain remains largely controversial. Here, we evaluated the ability of fetal neural stem cells (fNSC) to relieve chronic pain and, secondarily, their effects on motor recovery. Adult Wistar rats with traumatic SCI were treated, 10 days after injury, with intra-spinal injections of culture medium (sham) or fNSCs extracted from telencephalic vesicles (TV...
Source: Cell Research - July 12, 2019 Category: Cytology Authors: Batista CM, Mariano ED, Dale CS, Cristante AF, Britto LR, Otoch JP, Teixeira MJ, Morgalla M, Lepski G Tags: Neural Regen Res Source Type: research

Cell Res; +283 new citations
283 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2019/07/10PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - July 11, 2019 Category: Cytology Tags: Report Source Type: research

Cell Res; +131 new citations
131 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2019/07/06PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - July 6, 2019 Category: Cytology Tags: Report Source Type: research

Cell Res; +151 new citations
151 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2019/07/05PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - July 5, 2019 Category: Cytology Tags: Report Source Type: research

Cell Res; +128 new citations
128 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2019/07/03PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - July 4, 2019 Category: Cytology Tags: Report Source Type: research

Cell Res; +102 new citations
102 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2019/07/02PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - July 2, 2019 Category: Cytology Tags: Report Source Type: research

Cell Res; +141 new citations
141 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2019/07/01PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - July 1, 2019 Category: Cytology Tags: Report Source Type: research

Differential Activation of Fetal Hofbauer Cells in Primigravidas Is Associated with Decreased Birth Weight in Symptomatic Placental Malaria.
Abstract Background: Placental malaria is a leading global cause of low birth weight neonates, especially in first-time mothers. To better understand the role of innate immunity in placental malaria, we investigated the relationships between histopathological markers of placental malaria, fetal and maternal macrophage responses, and perinatal outcomes in a cross-sectional case control study of pregnant women presenting with symptomatic malaria at the time of delivery. Results: Primigravidas showed increased hemozoin deposition in placental villi (p=0.02), syncytiotrophoblasts (p=0.01), and fetal Hofbauer cell...
Source: Cell Research - June 14, 2019 Category: Cytology Authors: Gaw SL, Hromatka BS, Ngeleza S, Buarpung S, Ozarslan N, Tshefu A, Fisher SJ Tags: Malar Res Treat Source Type: research

Enzymatic Conversion of RBCs by α-N-Acetylgalactosaminidase from Spirosoma linguale.
Enzymatic Conversion of RBCs by α-N-Acetylgalactosaminidase from Spirosoma linguale. Enzyme Res. 2019;2019:6972835 Authors: Malinski TJ, Singh H Abstract Spirosoma linguale is a free-living nonpathogenic organism. Like many other bacteria, S. linguale produces a cell-associated α-N-acetylgalactosaminidase. This work was undertaken to elucidate the nature of this activity. The recombinant enzyme was produced, purified, and examined for biochemical attributes. The purified enzyme was ~50 kDa active as a homodimer in solution. It catalyzed hydrolysis of α-N-acetylgalactosamine at pH 7. ...
Source: Cell Research - June 14, 2019 Category: Cytology Authors: Malinski TJ, Singh H Tags: Enzyme Res Source Type: research

Unique Roles of Sphingolipids in Selected Malignant and Nonmalignant Lesions of Female Reproductive System.
Abstract Cancer develops as a result of the loss of self-control mechanisms by a cell; it gains the ability to induce angiogenesis, becomes immortal and resistant to cell death, stops responding to growth suppressor signals, and becomes capable of invasion and metastasis. Sphingolipids-a family of membrane lipids-are known to play important roles in the regulation of cell proliferation, the response to chemotherapeutic agents, and/or prevention of cancer. Despite the underlying functions of sphingolipids in cancer biology, their metabolism in different malignant tumors is poorly investigated. Some studies showed m...
Source: Cell Research - June 14, 2019 Category: Cytology Authors: Knapp P, Chomicz K, Świderska M, Chabowski A, Jach R Tags: Biomed Res Int Source Type: research

New insights into the transcriptional regulation of aquaporin-2 and the treatment of X-linked hereditary nephrogenic diabetes insipidus.
Abstract The kidney collecting duct (CD) is a tubular segment of the kidney where the osmolality and final flow rate of urine are established, enabling urine concentration and body water homeostasis. Water reabsorption in the CD depends on the action of arginine vasopressin (AVP) and a transepithelial osmotic gradient between the luminal fluid and surrounding interstitium. AVP induces transcellular water reabsorption across CD principal cells through associated signaling pathways after binding to arginine vasopressin receptor 2 (AVPR2). This signaling cascade regulates the water channel protein aquaporin-2 (AQP2)....
Source: Cell Research - June 14, 2019 Category: Cytology Authors: Jung HJ, Kwon TH Tags: Kidney Res Clin Pract Source Type: research

Adipose tissue-derived mesenchymal stromal cells for treating chronic kidney disease: A pilot study assessing safety and clinical feasibility.
Conclusion: Findings from this pilot study demonstrate that intravenous infusion of autologous expanded AT-MSCs into CKD patients was not associated with adverse effects and could benefit patients already undergoing standard medical treatment. PMID: 31189223 [PubMed] (Source: Cell Research)
Source: Cell Research - June 14, 2019 Category: Cytology Authors: Villanueva S, González F, Lorca E, Tapia A, López VG, Strodthoff R, Fajre F, Carreño JE, Valjalo R, Vergara C, Lecanda M, Bartolucci J, Figueroa FE, Khoury M Tags: Kidney Res Clin Pract Source Type: research

Karyopherin Alpha 2-Expressing Pancreatic Duct Glands and Intra-Islet Ducts in Aged Diabetic C414A-Mutant-CRY1 Transgenic Mice.
Abstract Our earlier studies demonstrated that cysteine414- (zinc-binding site of mCRY1-) alanine mutant mCRY1 transgenic mice (Tg mice) exhibit diabetes characterized by the reduction of β-cell proliferation and by β-cell dysfunction, presumably caused by senescence-associated secretory phenotype- (SASP-) like characters of islets. Earlier studies also showed that atypical duct-like structures in the pancreas developed age-dependently in Tg mice. Numerous reports have described that karyopherin alpha 2 (KPNA2) is highly expressed in cancers of different kinds. However, details of the expression of KPNA2...
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Okano S, Yasui A, Kanno SI, Satoh K, Igarashi M, Nakajima O Tags: J Diabetes Res Source Type: research

The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice.
The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice. J Diabetes Res. 2019;2019:9536032 Authors: Yang Y, Chen Y, Chen J, Zhang D, Wang J, Mao X, Wei X, Li X, Ma X, Liu C, Wang K Abstract Thyrotropin (TSH) is a modulator of glucose metabolism by binding to its receptor on pancreatic cells. We used thyrotropin receptor (TSHR) knockout mice (Tshr -/-) as a model of TSH deletion to study its function in pancreatic β cells. Tshr -/- mice had a similar body weight at birth compared with Tshr +/+ mice, but grew at a significantly slower rate until adulthood with adequate...
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Yang Y, Chen Y, Chen J, Zhang D, Wang J, Mao X, Wei X, Li X, Ma X, Liu C, Wang K Tags: J Diabetes Res Source Type: research

Vitamin C in Cancer Therapeutics and Metastasis.
Abstract There have been significant increased publications of preclinical studies and clinical trials of vitamin C (ascorbate) on cancer therapeutics in the past a few years. In this communication reflecting my personal opinions, I will highlight the main points of current research status; discuss impacts of vitamin C on immune cell function and inflammation at tumor microenvironment, and tumor metastasis; and propose stimulating questions and direction for future research in this area. PMID: 31179437 [PubMed] (Source: Cell Research)
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Xi D Tags: J Orthop Res Ther Source Type: research

Histomorphometric and immunohistochemical evaluation of angiogenesis in ischemia by tissue engineering in rats: Role of mast cells.
Abstract The aim of this study was to find a proper method for improvement of ischemic condition in the rat hind limb and also to observe the efficacy of cell engraftment with alginate/gelatin three-dimensional scaffolds. Eighteen male Wistar rats weighing 200 to 250 g were randomly divided into three groups (n = 6) including a) ischemia group; in which femoral artery was removed after ligation at the distance of 5 mm, b) scaffold group; in which hydrogel scaffold was added to the site of transected femoral artery and c) test group; in which in addition to hydrogel scaffold, mast cells (MCs) were also added (1 &ti...
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Amani S, Shahrooz R, Mortaz E, Hobbenaghi R, Mohammadi R, Baradar Khoshfetrat A Tags: Vet Res Forum Source Type: research

An investigation of the concurrency of anti-Neospora antibody and parasitemia in water buffalo (Bubalus bubalis) in northwest of Iran.
Abstract Neospora caninum is an obligate intracellular parasite causing abortion and reproductive failure in ruminants. Here, the seroprevalence of Neospora DNA and anti-Neospora antibodies and the correlation between the DNA and the antibody using polymerase chain reaction (PCR) and a new developed whole cell-based enzyme-linked immunosorbent assay (ELISA) in water buffalo (Bubalus bubalis) were investigated. To determine the level of anti-Neospora antibody, 83 serum samples were collected from buffaloes in the northwest of Iran. Plates were coated with 2 × 106 whole Neospora tachyzoites and the anti-Neospo...
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Rezvan H, Khaki A, Namavari M, Abedizadeh R Tags: Vet Res Forum Source Type: research

The Dosage of the Derivative of Clostridium Ghonii (DCG) Spores Dictates Whether an IFN γ/IL-9 or a Strong IFNγ Response Is Elicited in TC-1 Tumour Bearing Mice.
The Dosage of the Derivative of Clostridium Ghonii (DCG) Spores Dictates Whether an IFNγ/IL-9 or a Strong IFNγ Response Is Elicited in TC-1 Tumour Bearing Mice. Biomed Res Int. 2019;2019:1395138 Authors: Ni G, Wang Y, Good D, Yuan J, Pan X, Wei J, Liu X, Wei MQ Abstract Background: Anaerobic Clostridial spores (CG) cause significant oncolysis in hypoxic tumour microenvironment and result in tumour regression in both animal models and clinical trials. The immune mediated response plays a critical role in the antitumour effect by the anaerobic spore treatment. Method: Human papillomavir...
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Ni G, Wang Y, Good D, Yuan J, Pan X, Wei J, Liu X, Wei MQ Tags: Biomed Res Int Source Type: research

Identification of CTRP1 as a Prognostic Biomarker and Oncogene in Human Glioblastoma.
Abstract Introduction: Glioblastoma (GBM) is the most frequent and malignant type of primary brain tumors in adults. The valuable prognostic biomarkers and therapeutic targets for GBM remain to be elucidated. The association of adipokines with cancer has been well documented. The C1q/TNF-related protein 1 (CTRP1), a novel adipokine, belongs to the CTRP family. Methods: In the present study, the expression and potential roles of CTRP1 in GBM were explored based on in silico evaluation, including GEPIA, the Pathology Atlas of the Human Protein Atlas, cBioPortal, TIMER, and SurvExpress. The CCK8, transwell, and ...
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Chen L, Su G Tags: Biomed Res Int Source Type: research

Second-Generation Histamine H1 Receptor Antagonists Suppress Delayed Rectifier K+-Channel Currents in Murine Thymocytes.
Conclusions: Our study revealed for the first time that second-generation antihistamines, including cetirizine, fexofenadine, azelastine, and terfenadine, exert suppressive effects on lymphocyte Kv1.3-channels. The efficacy of these drugs may be related to their immunomodulatory mechanisms that reduce the synthesis of inflammatory cytokine. PMID: 31183371 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Saito K, Abe N, Toyama H, Ejima Y, Yamauchi M, Mushiake H, Kazama I Tags: Biomed Res Int Source Type: research

TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway.
Conclusions: This study suggests that TRPV1 served as a tumor suppressor in CRC and contributed to the development of novel therapy of CRC. PMID: 31183372 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Hou N, He X, Yang Y, Fu J, Zhang W, Guo Z, Hu Y, Liang L, Xie W, Xiong H, Wang K, Pang M Tags: Biomed Res Int Source Type: research

Integrated Bioinformatics Analysis of Master Regulators in Anaplastic Thyroid Carcinoma.
In conclusion, the TFs regulatory network provided a more detail molecular mechanism underlying ATC occurrence and progression. TFs including E2F7, FOXM1, CREB3L1, and NFYB were likely to be master regulators of ATC progression, suggesting their potential role as molecular therapeutic targets in ATC treatment. PMID: 31183379 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Pan Z, Li L, Fang Q, Qian Y, Zhang Y, Zhu J, Ge M, Huang P Tags: Biomed Res Int Source Type: research

IL-21 Expands HIV-1-Specific CD8+ T Memory Stem Cells to Suppress HIV-1 Replication In Vitro.
In this study, we quantified peripheral blood antigen-specific CD8+ TSCMs and then expanded HIV-1-specific TSCMs that targeted optimal antigen epitopes (SL9, IL9, and TL9) in the presence of interleukin- (IL-) 21 or IL-15. The suppressive capacity of the expanded CD8+ TSCMs on HIV-1 production was measured by assessing cell-associated viral RNA and performing viral outgrowth assays. We found that the number of unmutated TL9-specific CD8+ TSCMs positively correlated with the abundance of CD4+ T cells and that the expression of IFN-γ was higher in TL9-specific CD8+ TSCMs than that in non-TL9-specific CD8+ TSCMs. Moreov...
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Wu K, Zhang S, Zhang X, Li X, Hong Z, Yu F, Liu B, Pan T, Huang Z, Tang XP, Cai W, Xia J, Li X, Zhang H, Zhang Y, Li L Tags: J Immunol Res Source Type: research

Soluble Markers of Antibody Secreting Cell Function as Predictors of Infection Risk in Rheumatoid Arthritis.
Conclusions: Our study using soluble factors linked to B-cell/ASC activation and survival suggests that there is a paucity of ASC in a subset of RA patients and that this may be linked to altered antibody production and increased risk of infections. Further delineating the link between ASC and infection susceptibility in RA may optimize disease management and provide novel insights into disease pathogenesis that are susceptible to intervention. PMID: 31183387 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Gutierrez MJ, Desiderio SV, Wang NY, Darrah E, Cappelli L, Nino G, Jones M, Bingham CO Tags: J Immunol Res Source Type: research

A Single 48  mg Sucralose Sip Unbalances Monocyte Subpopulations and Stimulates Insulin Secretion in Healthy Young Adults.
This study was a randomized, placebo-controlled clinical trial. Volunteers randomly received 60 mL water as placebo (n = 20) or 48 mg sucralose dissolved in 60 mL water (n = 25), fifteen minutes prior to an OGTT. Blood samples were individually drawn every 15 minutes for 180 minutes for quantifying glucose and insulin concentrations. Monocyte subsets expressing CD11c and CD206 were measured at -15 and 180 minutes by flow cytometry. As compared to controls, volunteers receiving sucralose exhibited significant increases in serum insulin at 30, 45, and 180 minutes, whereas blood glucose values showed no s...
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Gómez-Arauz AY, Bueno-Hernández N, Palomera LF, Alcántara-Suárez R, De León KL, Méndez-García LA, Carrero-Aguirre M, Manjarrez-Reyna AN, Martínez-Reyes CP, Esquivel-Velázquez M, Ruiz-Barranco A, Baltazar-López N, Islas-Andrade S, Escobedo G, Mel Tags: J Immunol Res Source Type: research

New Insights on the Adjuvant Properties of the Leishmania infantum Eukaryotic Initiation Factor.
In this study, we assessed the adjuvant properties of recombinant Leishmania infantum eukaryotic initiation factor (LieIF) through in vitro and in vivo assays. LieIF was intraperitoneally administered in combination with the protein antigen ovalbumin (OVA), and the widely used alum was used as a reference adjuvant. Our in vitro studies using J774A.1 macrophages showed that LieIF induced stimulatory effects as demonstrated by the enhanced surface expression of CD80 and CD86 co-stimulatory molecules and the induced production of the immune mediators NO and MIP-1α. Additionally, LieIF co-administration with OVA in an in...
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Koutsoni OS, Barhoumi M, Guizani I, Dotsika E Tags: J Immunol Res Source Type: research

"Mesenchymal Stem Cells": Blurred Image of Developmental Origin, Cellular Niche and Traits with Development of Regenerative Therapy Over and Above.
"Mesenchymal Stem Cells": Blurred Image of Developmental Origin, Cellular Niche and Traits with Development of Regenerative Therapy Over and Above. Curr Stem Cell Res Ther. 2019;14(4):292 Authors: Trivanovic D Abstract PMID: 31184296 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - June 12, 2019 Category: Cytology Authors: Trivanovic D Tags: Curr Stem Cell Res Ther Source Type: research

The exploration of effect of terfenadine on Ca2+ signaling in renal tubular cells.
This study examined whether terfenadine altered Ca2+ signaling and caused cytotoxicity in Madin-Darby canine kidney (MDCK) renal tubular cells. The Ca2+-sensitive fluorescent dye fura-2 was used to measure [Ca2+]i. Cell viability was measured by the fluorescent reagent 4-[3-[4-lodophenyl]-2-4(4-nitrophenyl)-2H-5-tetrazolio-1,3-benzene disulfonate] water soluble tetrazolium-1 (WST-1) assay. Terfenadine at concentrations of 100-1000 μM induced [Ca2+]i rises concentration dependently. The response was reduced by approximately 35% by removing extracellular Ca2+. In Ca2+-free medium, treatment with the endoplasmic ret...
Source: Cell Research - June 11, 2019 Category: Cytology Authors: Cheng HH, Liang WZ, Kuo CC, Hao LJ, Chou CT, Jan CR Tags: J Recept Signal Transduct Res Source Type: research

Double-strand breaks in ribosomal RNA genes activate a distinct signaling and chromatin response to facilitate nucleolar restructuring and repair.
en DH Abstract The nucleolus is a nuclear sub-domain containing the most highly transcribed genes in the genome. Hundreds of human ribosomal RNA (rRNA) genes, located in the nucleolus, rely on constant maintenance. DNA double-strand breaks (DSBs) in rRNA genes activate the ATM kinase, repress rRNA transcription and induce nucleolar cap formation. Yet how ribosomal-DNA (rDNA) lesions are detected and processed remains elusive. Here, we use CRISPR/Cas9-mediated induction of DSBs and report a chromatin response unique to rDNA depending on ATM-phosphorylation of the nucleolar protein TCOF1 and recruitment of the MRE11...
Source: Cell Research - June 11, 2019 Category: Cytology Authors: Korsholm LM, Gál Z, Lin L, Quevedo O, Ahmad DA, Dulina E, Luo Y, Bartek J, Larsen DH Tags: Nucleic Acids Res Source Type: research

Trilayer scaffold with cardiosphere-derived cells for heart valve tissue engineering.
Abstract Natural polymers collagen, glycosaminoglycans, and elastin are promising candidate materials for heart valve tissue engineering scaffolds. This work produced trilayer scaffolds that resembled the layered structures of the extracellular matrices of native heart valves. The scaffolds showed anisotropic bending moduli (in both dry and hydrated statuses) depending on the loading directions (lower in the With Curvature direction than in the Against Curvature direction), which mimicked the characteristic behavior of the native heart valves. The interactions between cardiosphere-derived cells and the scaffolds w...
Source: Cell Research - June 11, 2019 Category: Cytology Authors: Chen Q, Bruyneel A, Carr C, Czernuszka J Tags: J Biomed Mater Res B Appl Biomater Source Type: research

Flurbiprofen inhibits androgen productions in rat immature Leydig cells.
In conclusion, flurbiprofen directly inhibits HSD3B activity and the expression levels of Srd5a1 and Akr1c14 in rat Leydig cells, thus leading to the reduction of androgen secretion. PMID: 31184881 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - June 11, 2019 Category: Cytology Authors: Wang Y, Zheng W, Shan Y, Qiu L, Dong Y, Ni C, Li X, Huang T, Zhu Q, Lian Q, Ge RS Tags: Chem Res Toxicol Source Type: research

Matrix Metalloproteinases in Renal Diseases: A Critical Appraisal.
ař V Abstract Matrix metalloproteinases (MMPs) are endopeptidases within the metzincin protein family that not only cleave extracellular matrix (ECM) components, but also process the non-ECM molecules, including various growth factors and their binding proteins. MMPs participate in cell to ECM interactions, and MMPs are known to be involved in cell proliferation mechanisms and most probably apoptosis. These proteinases are grouped into six classes: collagenases, gelatinases, stromelysins, matrilysins, membrane type MMPs, and other MMPs. Various mechanisms regulate the activity of MMPs, inhibition by tissue inhibi...
Source: Cell Research - June 11, 2019 Category: Cytology Authors: Zakiyanov O, Kalousová M, Zima T, Tesař V Tags: Kidney Blood Press Res Source Type: research

Elevated bradykinin receptor type 1 expression in postpartum acute myometritis: Possible involvement in augmented interstitial edema of the atonic gravid uterus.
CONCLUSION: We demonstrated the up-regulated expression of B1R in the myometrium and its positive correlation with histologically estimated interstitial edema, suggesting the contribution of the kinin-kallikrein-B1R system to the development of interstitial edema in PAM cases. PMID: 31179603 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - June 10, 2019 Category: Cytology Authors: Shen Y, Oda T, Tamura N, Kohmura-Kobayashi Y, Furuta-Isomura N, Yaguchi C, Uchida T, Suzuki K, Itoh H, Kanayama N Tags: J Obstet Gynaecol Res Source Type: research

Immune Correlates of Herpes Zoster in People Living with HIV on Effective Antiretroviral Therapy.
Conclusions Increased circulating Treg and decreased VZV-specific T cell immune responses were associated with the risk of HZ in PLHIV. The kinetics of Treg over time, but not of VZV-CTL, paralleled the natural history of HZ, whose incidence decreases over time on effective ART. PMID: 31179712 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - June 10, 2019 Category: Cytology Authors: Tovar-Salazar A, McKhann A, Chen H, Bosch RJ, Weinberg A Tags: AIDS Res Hum Retroviruses Source Type: research

Tizanidine hydrochloride exhibits a cytotoxic effect on osteosarcoma cells through the PI3K/AKT signaling pathway.
CONCLUSIONS: THC may play a novel role in OS cell cytotoxicity, and these findings suggest a potent therapeutic strategy for OS treatment. PMID: 31179788 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - June 10, 2019 Category: Cytology Authors: Xing XW, Sun YF, Zhao J, Pan ZX, Jiang WX Tags: J Int Med Res Source Type: research

A phase II study of the combination of docetaxel and bevacizumab for previously treated non-small cell lung cancer.
CONCLUSIONS: The combination of DOC and BV often resulted in serious neutropenia, suggesting that this regimen is difficult to tolerate. PMID: 31179803 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - June 10, 2019 Category: Cytology Authors: Shimizu T, Nakagawa Y, Asai Y, Tsujino I, Takahashi N, Gon Y Tags: J Int Med Res Source Type: research

Limpid hydrogels from β-turn motif-connected tandem repeats of Aβ16-22.
Limpid hydrogels from β-turn motif-connected tandem repeats of Aβ16-22. Soft Matter. 2019 Jun 10;: Authors: Datta D, Kumar V, Kumar S, Nagaraj R, Chaudhary N Abstract Self-assembling peptides constitute an important class of functional biomaterials. A number of short amyloidogenic stretches have been identified from amyloid proteins. Such peptides, as such or through subtle modifications, can turn out to be promising candidates for functional biomaterials. End-capped Aβ16-22, the well-studied amyloidogenic stretch from β-amyloid, is reported to be non-hydrogelating up to 20 mM conc...
Source: Cell Research - June 10, 2019 Category: Cytology Authors: Datta D, Kumar V, Kumar S, Nagaraj R, Chaudhary N Tags: Soft Matter Source Type: research

Characterization of the stimulators of protein-directed ribosomal frameshifting in Theiler's murine encephalomyelitis virus.
We present an extensive mutational analysis of the frameshift stimulators (mRNA signals and 2A protein) analysing activity in in vitro translation, electrophoretic mobility shift and in vitro ribosome pausing assays. We also investigate the PRF mRNA signal with RNA structure probing. Our results substantially extend previous characterization of protein-stimulated PRF. PMID: 31180502 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - June 10, 2019 Category: Cytology Authors: Napthine S, Bell S, Hill CH, Brierley I, Firth AE Tags: Nucleic Acids Res Source Type: research

Targeting the SPOCK1-snail/slug axis-mediated epithelial-to-mesenchymal transition by apigenin contributes to repression of prostate cancer metastasis.
CONCLUSIONS: Levels of SPOCK1 increase with the progression of human PCa which suggests that SPOCK1 may act as a prognostic marker or therapeutic target for patients with PCa. Suppression of SPOCK1-mediated EMT signaling contributes to the antiproliferative and antimetastatic activities of API in vitro and in vivo. PMID: 31182131 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - June 10, 2019 Category: Cytology Authors: Chien MH, Lin YW, Wen YC, Yang YC, Hsiao M, Chang JL, Huang HC, Lee WJ Tags: J Exp Clin Cancer Res Source Type: research

PLEK2 promotes gallbladder cancer invasion and metastasis through EGFR/CCL2 pathway.
CONCLUSIONS: On the basis of these collective data, we propose that PLEK2 promotes the invasion and metastasis of GBC by EGFR/CCL2 pathway and PLEK2 can serve as a potential therapeutic target for GBC treatment. PMID: 31182136 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - June 10, 2019 Category: Cytology Authors: Shen H, He M, Lin R, Zhan M, Xu S, Huang X, Xu C, Chen W, Yao Y, Mohan M, Wang J Tags: J Exp Clin Cancer Res Source Type: research

AKR1C1 controls cisplatin-resistance in head and neck squamous cell carcinoma through cross-talk with the STAT1/3 signaling pathway.
CONCLUSIONS: AKR1C1 is a crucial regulator for cisplatin-resistance in HNSCC and also poor prognostic marker for patients. Targeting the AKR1C1-STAT axis may provide a new therapeutic strategy to treat patients who are refractory to cisplatin treatment. PMID: 31182137 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - June 10, 2019 Category: Cytology Authors: Chang WM, Chang YC, Yang YC, Lin SK, Chang PM, Hsiao M Tags: J Exp Clin Cancer Res Source Type: research