Resveratrol-Loaded Polydimethylsiloxane-Silica Hybrid Materials: Synthesis, Characterization, and Antitumoral Activity
Polymers (Basel). 2024 Mar 22;16(7):879. doi: 10.3390/polym16070879.ABSTRACTIn this work, hybrid materials within the polydimethylsiloxane-silica (PDMS-SiO2) system, synthesized via the sol-gel method, were developed and characterized for their potential to incorporate and release the bioactive compound resveratrol (RES). RES was incorporated into the materials with a high loading efficiency (>75%) using the rotary evaporator technique. This incorporation induced the amorphization of RES, resulting in enhanced solubility and in vitro release when compared to the free polyphenolic compound. The release profiles displayed...
Source: Cell Research - April 13, 2024 Category: Cytology Authors: Sofia Viegas Diogo Marinheiro Ver ónica Bastos Ana L Daniel-da-Silva Ricardo Vieira Helena Oliveira Jos é Carlos Almeida B árbara J M L Ferreira Source Type: research

Transient Receptor Potential Ankyrin 1 Ion Channel Is Expressed in Osteosarcoma and Its Activation Reduces Viability
Int J Mol Sci. 2024 Mar 28;25(7):3760. doi: 10.3390/ijms25073760.ABSTRACTOsteosarcoma is a highly malignant, painful cancer with poor treatment opportunities and a bad prognosis. Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are non-selective cation channels that have been of great interest in cancer, as their expression is increased in some malignancies. In our study we aim to characterize the expression and functionality of the TRPA1 and TRPV1 channels in human and mouse osteosarcoma tissues and in a mouse cell line. TRPA1/Trpa1 and TRPV1/Trpv1 mRNA expressions were demonstrated by PCR ...
Source: Cell Research - April 13, 2024 Category: Cytology Authors: Lina Hudhud Katalin Rozmer Ang éla Kecskés Krisztina Poh óczky No émi Bencze Krisztina Buz ás Éva Szőke Zsuzsanna Helyes Source Type: research

Molecular heterogeneity of quiescent melanocyte stem cells revealed by single-cell RNA-sequencing
Pigment Cell Melanoma Res. 2024 Apr 13. doi: 10.1111/pcmr.13169. Online ahead of print.ABSTRACTMelanocyte stem cells (McSCs) of the hair follicle are a rare cell population within the skin and are notably underrepresented in whole-skin, single-cell RNA sequencing (scRNA-seq) datasets. Using a cell enrichment strategy to isolate KIT+/CD45- cells from the telogen skin of adult female C57BL/6J mice, we evaluated the transcriptional landscape of quiescent McSCs (qMcSCs) at high resolution. Through this evaluation, we confirmed existing molecular signatures for qMcCS subpopulations (e.g., Kit+, Cd34+/-, Plp1+, Cd274+/-, Thy1+, ...
Source: Cell Research - April 13, 2024 Category: Cytology Authors: Joseph W Palmer Nilesh Kumar Luye An Andrew C White M Shahid Mukhtar Melissa L Harris Source Type: research

Clinical Value of Human Endogenous Retrovirus-H Long Terminal Repeat Associating 2 (HHLA2) in Small Cell Lung Cancer
Conclusion: The new immune molecule HHLA2 may be an ideal clinical biomarker for predicting SCLC progression and could serve as a new immunotherapy target in SCLC.PMID:38613340 | DOI:10.1177/15330338241240683 (Source: Cell Research)
Source: Cell Research - April 13, 2024 Category: Cytology Authors: Xiuqin Zhang Yan Qin Xu Chen Mengrui Xiong Song Shu Source Type: research

Predictive Value of NLR and PLR in Driver-Gene-Negative Advanced Non-Small Cell Lung Cancer Treated with PD-1/PD-L1 Inhibitors: A Single Institutional Cohort Study
CONCLUSION: Low baseline NLR and PLR levels are significantly associated with better response in advanced NSCLC patients treated with PD-1/PD-L1 inhibitors, which may be indicators to predict the efficacy of immunotherapy in advanced NSCLC with driver-gene-negative.PMID:38613344 | DOI:10.1177/15330338241246651 (Source: Cell Research)
Source: Cell Research - April 13, 2024 Category: Cytology Authors: Qi Yuan Chunhua Xu Wei Wang Qian Zhang Source Type: research

Identification of Novel Cuproptosis-Related Genes Mediating the Prognosis and Immune Microenvironment in Cholangiocarcinoma
CONCLUSIONS: Collectively, we established and verified an effective prognostic model that could separate cholangiocarcinoma patients into 2 heterogeneous cuproptosis subtypes based on the molecular or protein characteristics of 10 cuproptosis-related genes. These findings may provide potential benefits for unveiling molecular characteristics and defining subgroups could improve the early diagnosis and individualized treatment of cholangiocarcinoma patients.PMID:38613350 | DOI:10.1177/15330338241239139 (Source: Cell Research)
Source: Cell Research - April 13, 2024 Category: Cytology Authors: Qiang Liu Jianpeng Zhu Zhicheng Huang Xiaofeng Zhang Jianfeng Yang Source Type: research

The long non-coding RNA Meg3 mediates imprinted gene expression during stem cell differentiation
Nucleic Acids Res. 2024 Apr 13:gkae247. doi: 10.1093/nar/gkae247. Online ahead of print.ABSTRACTThe imprinted Dlk1-Dio3 domain comprises the developmental genes Dlk1 and Rtl1, which are silenced on the maternal chromosome in different cell types. On this parental chromosome, the domain's imprinting control region activates a polycistron that produces the lncRNA Meg3 and many miRNAs (Mirg) and C/D-box snoRNAs (Rian). Although Meg3 lncRNA is nuclear and associates with the maternal chromosome, it is unknown whether it controls gene repression in cis. We created mouse embryonic stem cells (mESCs) that carry an ectopic poly(A)...
Source: Cell Research - April 13, 2024 Category: Cytology Authors: Sabina Farhadova Amani Ghousein Fran çois Charon Caroline Surcis Melisa Gomez-Velazques Clara Roidor Flavio Di Michele Maud Borensztein Albertina De Sario Cyril Esnault Daan Noordermeer Benoit Moindrot Robert Feil Source Type: research

Activity reconstitution of Kre33 and Tan1 reveals a molecular ruler mechanism in eukaryotic tRNA acetylation
Nucleic Acids Res. 2024 Apr 13:gkae262. doi: 10.1093/nar/gkae262. Online ahead of print.ABSTRACTRNA acetylation is a universal post-transcriptional modification that occurs in various RNAs. Transfer RNA (tRNA) acetylation is found at position 34 (ac4C34) in bacterial tRNAMet and position 12 (ac4C12) in eukaryotic tRNASer and tRNALeu. The biochemical mechanism, structural basis and functional significance of ac4C34 are well understood; however, despite being discovered in the 1960s and identification of Kre33/NAT10 and Tan1/THUMPD1 as modifying apparatuses, ac4C12 modification activity has never been reconstituted for nearl...
Source: Cell Research - April 13, 2024 Category: Cytology Authors: Chun-Rui Ma Na Liu Hong Li Hong Xu Xiao-Long Zhou Source Type: research

Identification of New Pathogenic Variants of Hereditary Diffuse Gastric Cancer
CONCLUSION: The RHOA mutation (R129W) emerges as a potential causative gene for HDGC, but only in one family, indicating a need for further studies to understand its role in HDGC pathogenesis fully.PMID:38605661 | DOI:10.4143/crt.2024.328 (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Seung-Young Oh Giyong Jang Jaeryuk Kim Kyoung-Yun Jeong Hyun Myong Kim Yoon Jin Kwak Seong-Ho Kong Do Joong Park Hyuk-Joon Lee Sung-Yup Cho Jong-Il Kim Han-Kwang Yang Source Type: research

Prognostic Significance of Bulky Nodal Disease in Anal Cancer Management: A Multi Institutional Study
CONCLUSION: Patients with bulky nodal disease treated with standard chemoradiotherapy experienced poor survival outcomes, indicating the potential necessity for further treatment intensification.PMID:38605662 | DOI:10.4143/crt.2024.258 (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Seok-Joo Chun Eunji Kim Won Il Jang Mi-Sook Kim Hyun-Cheol Kang Byoung Hyuck Kim Eui Kyu Chie Source Type: research

Constitutive hypercoagulability in pediatric sickle cell disease patients with hemoglobin SS genotype
CONCLUSION: Our results indicate that pediatric SCD patients with the HbSS genotype have constitutively activated hemostasis relative to HbSC patients and healthy children. It remains to be determined how treatments that improve clinical outcomes in SCD patients affect this constitutively hypercoagulable state.PMID:38605827 | PMC:PMC11004888 | DOI:10.1016/j.rpth.2024.102374 (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Raizl G Sussman Joy Mburu MacGregor Steele Annie Bang Jeremy Friedman Ran Goldman Melanie Kirby Margaret L Rand Victor S Blanchette Fred G Pluthero Suzan Williams Walter H A Kahr Source Type: research

Targeted therapeutic options in early and metastatic NSCLC-overview
Pathol Oncol Res. 2024 Mar 28;30:1611715. doi: 10.3389/pore.2024.1611715. eCollection 2024.ABSTRACTThe complex therapeutic strategy of non-small cell lung cancer (NSCLC) has changed significantly in recent years. Disease-free survival increased significantly with immunotherapy and chemotherapy registered in perioperative treatments, as well as adjuvant registered immunotherapy and targeted therapy (osimertinib) in case of EGFR mutation. In oncogenic-addictive metastatic NSCLC, primarily in adenocarcinoma, the range of targeted therapies is expanding, with which the expected overall survival increases significantly, measure...
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Gabriella G álffy Éva Morócz R éka Korompay R éka Hécz R éka Bujdosó Rita Pusk ás T ímea Lovas Eszter G áspár Kamel Yahya P éter Király Zolt án Lohinai Source Type: research

Intestinal Langerhans cell histiocytosis presenting with symptoms similar to inflammatory bowel disease: a case report
CONCLUSION: The clinical symptoms of Langerhans cell histiocytosis involving the gastrointestinal tract are not specific and may resemble symptoms observed in inflammatory bowel disease and other primary gastrointestinal tumors. Therefore, in cases of infants presenting with inflammatory gastrointestinal symptoms that do not resolve after treatment, a biopsy is essential to obtain a differential diagnosis.PMID:38605931 | PMC:PMC11007090 | DOI:10.3389/pore.2024.1611705 (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Yuqing Liu Zhenwei Chen Lu Wang Baizhou Li Source Type: research

Peptide Extract from Red Kidney Beans, < em > Phaseolus vulgaris < /em > (Fabaceae), Shows Promising Antimicrobial, Antibiofilm, and Quorum Sensing Inhibitory Effects
In this study, peptides were extracted from the seeds of Phaseolus vulgaris (Fabaceae), and the antimicrobial activities of the extract were evaluated using microbroth dilution against five different microorganisms. The extract showed antimicrobial activity against all tested organisms with minimum inhibitory concentrations (MIC) of 2.5 mg/mL, except for Candida albicans and Pseudomonas aeruginosa, which had MICs of 1.25 mg/mL. The extract was also bacteriostatic for all tested organisms. The crude peptide extract from Phaseolus vulgaris was further studied for its antibiofilm activity against Pseudomonas aeruginosa, a com...
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Jennifer Tetteh De-Youngster Wereko Brobbey Kofi Junior Osei Azumah Ayamah Michael Konney Laryea Godfred Darko Lawrence Sheringham Borquaye Source Type: research

Exploring the landscape of stem cell extracellular vesicle research for angiogenesis: A bibliometric analysis from 2003 to 2023
CONCLUSION: In this comprehensive review, global trends in SC-EVs for angiogenesis were analyzed. The analysis of journals, institutions, references, and keywords could assist researchers in deciding on the direction of research. The role of SC-EVs in promoting angiogenesis during wound healing and repair represents an emerging research focus.PMID:38606725 | DOI:10.1111/srt.13694 (Source: Cell Research)
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Yuchong Wang Changjiang Zhao Rong Guo Tingting Xi Jiachao Xiong Lingling Jia Source Type: research