Metabolic engineering of Escherichia coli for the utilization of ethanol.
Conclusions: This study demonstrated the possibility of using ethanol as the carbon source by engineered E. coli strains. It can serve as the basis for the construction of more robust strains in the future though the catabolic capacity of ethanol should be further improved. PMID: 31993378 [PubMed] (Source: Cell Research)
Source: Cell Research - January 31, 2020 Category: Cytology Authors: Cao Y, Mu H, Guo J, Liu H, Zhang R, Liu W, Xian M, Liu H Tags: J Biol Res (Thessalon) Source Type: research

Improved delivery of doxorubicin using rationally designed PEGylated platinum nanoparticles for the treatment of melanoma.
Abstract Efficient delivery of chemotherapeutic drugs to tumor cells is one of the crucial issues for modern day cancer therapy. In this article, we report the synthesis of poly ethylene glycol (PEG) assisted colloidal platinum nanoparticles (PtNPs) by borohydride reduction method at room temperature. PtNPs are stable at room temperature for more than 2 years and are stable in serum and phosphate buffer (pH = 7.4) solution for one week. PtNPs show biocompatibility in different normal cell lines (in vitro) and chicken egg embryonic model (ex vivo). Further, we designed and fabricated PtNPs-based drug delivery s...
Source: Cell Research - January 13, 2020 Category: Cytology Authors: Mukherjee S, Kotcherlakota R, Haque S, Bhattacharya D, Kumar JM, Chakravarty S, Patra CR Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Cell Res; +142 new citations
142 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2020/01/12PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - January 12, 2020 Category: Cytology Tags: Report Source Type: research

Cell Res; +145 new citations
145 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2020/01/10PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - January 11, 2020 Category: Cytology Tags: Report Source Type: research

Cell Res; +152 new citations
152 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2020/01/09PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - January 9, 2020 Category: Cytology Tags: Report Source Type: research

Cell Res; +140 new citations
140 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2020/01/08PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - January 8, 2020 Category: Cytology Tags: Report Source Type: research

Stress or injury induces cellular plasticity in salivary gland acinar cells.
Abstract Salivary gland function is severely disrupted by radiation therapy used to treat patients diagnosed with head and neck cancer and by Sjögren's syndrome. The resulting condition, which results in xerostomia or dry mouth, is due to irreversible loss of the secretory acinar cells within the major salivary glands. There are presently no treatments for the resolution of xerostomia. Cell-based approaches could be employed to repopulate acinar cells in the salivary gland but investigations into potential therapeutic strategies are limited by the challenges of maintaining and expanding acinar cells in vitro....
Source: Cell Research - January 4, 2020 Category: Cytology Authors: Shubin AD, Sharipol A, Felong TJ, Weng PL, Schutrum BE, Joe DS, Aure MH, Benoit DSW, Ovitt CE Tags: Cell Tissue Res Source Type: research

Protective Effects of Melatonin on Methamphetamine-Induced Blood-Brain Barrier Dysfunction in Rat Model.
This study was designed to investigate the protective effects of melatonin on methamphetamine (METH)-induced alterations of BBB integrity. Wistar rats were randomly distributed into groups and underwent melatonin pretreatment and escalating-high doses of METH treatment. Immunohistochemistry was performed to demonstrate the BBB leakage. Protein and RNA samples were isolated from hippocampal and prefrontal cortical tissues and measured expression levels of molecular markers associated with BBB structural components and inflammatory processes. METH provoked the loss of zonula occludens (ZO)-1, occludin, and claudin-5 tight ju...
Source: Cell Research - January 4, 2020 Category: Cytology Authors: Namyen J, Permpoonputtana K, Nopparat C, Tocharus J, Tocharus C, Govitrapong P Tags: Neurotox Res Source Type: research

Hydrogen Sulfide Attenuates the Neurotoxicity in the Animal Model of Fetal Alcohol Spectrum Disorders.
Abstract Fetal alcohol spectrum disorder (FASD), which is caused by prenatal alcohol exposure, can result in cell death in specific brain regions. Alcohol-induced neurocognitive defects offspring's are included with activation of oxidative-inflammatory cascade followed with wide apoptotic neurodegeneration in many brain's regions such as hippocampus. According to the latest studies, H2S (hydrogen sulfide) can protect neuronal cells via antioxidant, anti-inflammatory, and anti-apoptotic mechanisms in different animal models. Therefore, we aimed to evaluate the protective effects of H2S on ethanol-induced neuroinfla...
Source: Cell Research - January 4, 2020 Category: Cytology Authors: Mohseni F, Bagheri F, Khaksari M Tags: Neurotox Res Source Type: research

The Potential Role of PKA/CREB Signaling Pathway Concerned with Gastrodin Administration on Methamphetamine-Induced Conditioned Place Preference Rats and SH-SY5Y Cell Line.
Abstract To investigate the effects of gastrodin (GAS) on methamphetamine (MA)-induced conditioned place preference (CPP) in rats and explore its potential mechanisms. MA (10 mg/kg) was initially injected intraperitoneally (i.p.) in rats, after which they were administered either MA or saline alternately from day 4 to 13 (D4-13) for 10 days, followed by treatment with GAS (10 or 20 mg/kg, i.p.) on D15-21 for 7 days. The rats underwent CPP testing after MA and GAS treatment. In vitro, SH-SY5Y cells were exposed to MA (2.0 mM) for 24 h, followed by treatment with GAS (2.0 or 4.0 mM...
Source: Cell Research - January 4, 2020 Category: Cytology Authors: Yang GM, Li L, Xue FL, Ma CL, Zeng XF, Zhao YN, Zhang DX, Yu Y, Yan QW, Zhou YQ, Hong SJ, Li LH Tags: Neurotox Res Source Type: research

The Dephosphorylation of p70S6 (Thr389) Kinase as a Marker of L-Glutamate-Induced Excitotoxicity Related to Diabetes Disturbances-an Unconventional In Vitro Model.
Abstract Excitotoxicity is a modern clinical condition included in the pathogenesis of Alzheimer's disease. It is connected with diabetic disturbance, and it is still being analyzed in the context of the participation of the PI3K/mTOR pathway. A very important protein belonging to this pathway is p70S6K, whose activation promotes the pathogenesis of type 2 diabetes by the induction of insulin resistance. The study model was based on a PC12 cell line, derived from the pheochromocytoma of a rat adrenal medulla, cultured in RPMI 1640. The three reagents were used in different concentrations to create the model of exc...
Source: Cell Research - January 4, 2020 Category: Cytology Authors: Rorbach-Dolata A, Kicinska A, Piwowar A Tags: Neurotox Res Source Type: research

First report of Enterobacter hormaechei with respiratory disease in calves.
CONCLUSIONS: To the best of our knowledge, this is the first case report of calves with respiratory disease that was associated with E. hormaechei, and multi-drug resistance was observed in isolates. PMID: 31900161 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Wang Z, Duan L, Liu F, Hu Y, Leng C, Kan Y, Yao L, Shi H Tags: BMC Vet Res Source Type: research

Reproducibility of GMP-compliant production of therapeutic stressed peripheral blood mononuclear cell-derived secretomes, a novel class of biological medicinal products.
CONCLUSIONS: We are the first to demonstrate the consistent production of cell-derived, yet cell-free secretome as a biological medicinal product. The results from this study provide the basis for selecting appropriate quality control parameters for GMP-compliant production of therapeutic cell secretomes and pave the way for future clinical trials employing secretomes in tissue regenerative medicine. PMID: 31900195 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Laggner M, Gugerell A, Bachmann C, Hofbauer H, Vorstandlechner V, Seibold M, Gouya Lechner G, Peterbauer A, Madlener S, Demyanets S, Sorgenfrey D, Ostler T, Erb M, Mildner M, Ankersmit HJ Tags: Stem Cell Res Ther Source Type: research

Long non-coding RNA FER1L4 promotes osteogenic differentiation of human periodontal ligament stromal cells via miR-874-3p and vascular endothelial growth factor A.
CONCLUSION: FER1L4 positively regulates the osteogenic differentiation of PDLSCs via miR-874-3p and VEGFA. Our study provides a promising target for enhancing the osteogenic potential of PDLSCs and periodontal regeneration. PMID: 31900200 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Huang Y, Han Y, Guo R, Liu H, Li X, Jia L, Zheng Y, Li W Tags: Stem Cell Res Ther Source Type: research

Small extracellular vesicles derived from embryonic stem cells restore ovarian function of premature ovarian failure through PI3K/AKT signaling pathway.
CONCLUSION: Our findings suggested that ESCs-sEVs could improve ovarian function by regulating the PI3K/AKT signaling pathway, which could provide a promising clinical therapy for POF. PMID: 31900201 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Liu M, Qiu Y, Xue Z, Wu R, Li J, Niu X, Yuan J, Wang Y, Wu Q Tags: Stem Cell Res Ther Source Type: research

Two-hybrid screening of FAM13A protein partners in lung epithelial cells.
Abstract OBJECTIVES: Family with sequence similarity 13 member A (FAM13A) genetic variants have been associated with several chronic respiratory diseases including chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF) and lung cancer. The FAM13A protein includes a RhoGTPase activating protein (RhoGAP) domain known to participate in various cellular mechanisms including cell proliferation. While intensive genomic studies have been performed to reveal its involvement in lung diseases, the biological role of FAM13A protein is still not completely elucidated. RESU...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Ruffin M, Thompson KE, Corvol H, Guillot L Tags: BMC Res Notes Source Type: research

Prokaryotic ribosomal RNA stimulates zebrafish embryonic innate immune system.
Abstract OBJECTIVES: Cell-culture studies reported that prokaryotic RNA molecules among the various microbe-associated molecular patterns (MAMPs) were uniquely present in live bacteria and were categorized as viability-associated MAMPs. They also reported that specific nucleotide modifications are instrumental in the discrimination between self and nonself RNAs. The aim of this study was to characterize the in vivo immune induction potential of prokaryotic and eukaryotic ribosomal RNAs (rRNAs) using zebrafish embryos as novel whole animal model system. Additionally, we aimed to test the possible role of rRNA ...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Basu A, Yoshihama M, Uechi T, Kenmochi N Tags: BMC Res Notes Source Type: research

LncRNA LINC00662 promotes colon cancer tumor growth and metastasis by competitively binding with miR-340-5p to regulate CLDN8/IL22 co-expression and activating ERK signaling pathway.
CONCLUSION: LINC00662 overexpression promoted the occurrence and development of colon cancer by competitively binding with miR-340-5p to regulate CLDN8/IL22 co-expression and activating ERK signaling pathway. PMID: 31900207 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Cheng B, Rong A, Zhou Q, Li W Tags: J Exp Clin Cancer Res Source Type: research

Effect of hCMSCs and liraglutide combination in ALI through cAMP/PKAc/ β-catenin signaling pathway.
CONCLUSIONS: These results reveal that the combination of hCMSCs and liraglutide might be an effective strategy for ALI treatment. PMID: 31900217 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Feng Y, Wang L, Ma X, Yang X, Don O, Chen X, Qu J, Song Y Tags: Stem Cell Res Ther Source Type: research

Extracellular vesicles for acute kidney injury in preclinical rodent models: a meta-analysis.
Abstract INTRODUCTION: Extracellular vesicles (EVs), especially stem cell-derived EVs, have emerged as a potential novel therapy for acute kidney injury (AKI). However, their effects remain incompletely understood. Therefore, we performed this meta-analysis to systematically review the efficacy of EVs on AKI in preclinical rodent models. METHODS: We searched PubMed, EMBASE, and the Web of Science up to March 2019 to identify studies that reported the treatment effects of EVs in a rodent AKI model. The primary outcome was serum creatinine (Scr) levels. The secondary outcomes were the blood urea nitrogen (BUN) ...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Liu C, Wang J, Hu J, Fu B, Mao Z, Zhang H, Cai G, Chen X, Sun X Tags: Stem Cell Res Ther Source Type: research

Preconditioning of mesenchymal stromal cells with low-intensity ultrasound: influence on chondrogenesis and directed SOX9 signaling pathways.
CONCLUSIONS: Collectively, preconditioning of MSCs by cLIUS resulted in the nuclear localization of SOX9, phosphorylation of ERK1/2 and disruption of actin filaments, and the expression of SOX9 was dependent on the phosphorylation of ERK1/2 under cLIUS. PMID: 31900222 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Sahu N, Budhiraja G, Subramanian A Tags: Stem Cell Res Ther Source Type: research

Aerobic and resistance training enhances endothelial progenitor cell function via upregulation of caveolin-1 in mice with type 2 diabetes.
CONCLUSION: Combined AT and RT is an effective way to improve EPC function through upregulation of caveolin-1 in mice with type 2 diabetes. PMID: 31900223 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Zhai L, Liu Y, Zhao W, Chen Q, Guo T, Wei W, Luo Z, Huang Y, Ma C, Huang F, Dai X Tags: Stem Cell Res Ther Source Type: research

Dopamine D1 receptor-mediated activation of the ERK signaling pathway is involved in the osteogenic differentiation of bone mesenchymal stem cells.
CONCLUSIONS: Taken together, these results suggest that ERK signaling may play an essential role in coordinating the DA-induced osteogenic differentiation of BMSCs by D1 receptor activation. PMID: 31900224 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Wang CX, Ge XY, Wang MY, Ma T, Zhang Y, Lin Y Tags: Stem Cell Res Ther Source Type: research

Long noncoding RNA HULC accelerates the growth of human liver cancer stem cells by upregulating CyclinD1 through miR675-PKM2 pathway via autophagy.
CONCLUSIONS: It reveals the key molecular signaling pathways for HULC and provides important basic information for finding effective tumor therapeutic targets based on HULC. PMID: 31900225 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Wang C, Jiang X, Li X, Song S, Meng Q, Wang L, Lu Y, Xin X, Pu H, Gui X, Li T, Lu D Tags: Stem Cell Res Ther Source Type: research

Cellular therapy of corneal epithelial defect by adipose mesenchymal stem cell-derived epithelial progenitors.
CONCLUSION: Human ADSC-derived epithelial-like cells, via MET, recovered the CE from PED associated with LSCD. ADSC can be a viable adult stem cell source for potential autologous epithelial cell-based therapy for corneal surface disorders. PMID: 31900226 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Bandeira F, Goh TW, Setiawan M, Yam GH, Mehta JS Tags: Stem Cell Res Ther Source Type: research

Age-dependent impairment of adipose-derived stem cells isolated from horses.
CONCLUSIONS: Obtained data suggest that ASCs derived from horses older than 5 years old exhibited several molecular alternations which markedly limit their regenerative capacity. The results provide valuable information that allows for a better understanding of the molecular events occurring in ASCs in the course of aging and may help to identify new potential drug targets to restore their regenerative potential. PMID: 31900232 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Alicka M, Kornicka-Garbowska K, Kucharczyk K, Kępska M, Rӧcken M, Marycz K Tags: Stem Cell Res Ther Source Type: research

Alteration of fatty acid oxidation by increased CPT1A on replicative senescence of placenta-derived mesenchymal stem cells.
CONCLUSIONS: These findings suggest that alteration of FAO by increased CPT1A plays an important role in mitochondrial dysfunction and senescence of PD-MSCs during long-term cultivation. PMID: 31900237 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Seok J, Jung HS, Park S, Lee JO, Kim CJ, Kim GJ Tags: Stem Cell Res Ther Source Type: research

Influence of mesenchymal stem cell-derived extracellular vesicles in vitro and their role in ageing.
CONCLUSIONS: MSC-derived extracellular vesicles affected the behaviour of MSC cultures, based on their composition, which could be modified in vitro. These experiments represented the basis for the development of new therapies against ageing-associated diseases using MSC-derived extracellular vesicles. PMID: 31900239 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Fafián-Labora J, Morente-López M, Sánchez-Dopico MJ, Arntz OJ, van de Loo FAJ, De Toro J, Arufe MC Tags: Stem Cell Res Ther Source Type: research

Pro-inflammatory cytokines induce cell death, inflammatory responses, and endoplasmic reticulum stress in human iPSC-derived beta cells.
CONCLUSIONS: Our data show that human iPSC-derived beta cells respond to pro-inflammatory cytokines IL-1β + IFNγ and IFNα, by activating the same pathogenic processes as adult human primary beta cells. These cells thus represent a valuable tool for future research on the pathogenesis of type 1 diabetes. PMID: 31900242 [PubMed - in process] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Demine S, Schiavo AA, Marín-Cañas S, Marchetti P, Cnop M, Eizirik DL Tags: Stem Cell Res Ther Source Type: research

Epigenetic targeting of tert-associated gene expression signature in human neuroblastoma with tert overexpression.
EPIGENETIC TARGETING OF TERT-ASSOCIATED GENE EXPRESSION SIGNATURE IN HUMAN NEUROBLASTOMA WITH TERT OVEREXPRESSION. Cancer Res. 2020 Jan 03;: Authors: Huang M, Zeki J, Sumarsono N, Coles GL, Taylor JS, Danzer E, Bruzoni M, Hazard FK, Lacayo NJ, Sakamoto KM, Dunn JCY, Spunt SL, Chiu B Abstract Neuroblastoma is a deadly pediatric solid tumor with infrequent recurrent somatic mutations. Particularly, the pathophysiology of tumors without MYCN amplification remains poorly defined. Utilizing an unbiased approach, we performed gene set enrichment analysis of RNA-seq data from 498 neuroblastoma patients and r...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Huang M, Zeki J, Sumarsono N, Coles GL, Taylor JS, Danzer E, Bruzoni M, Hazard FK, Lacayo NJ, Sakamoto KM, Dunn JCY, Spunt SL, Chiu B Tags: Cancer Res Source Type: research

HIF-2-induced long non-coding RNA RAB11B-AS1 promotes hypoxia-mediated angiogenesis and breast cancer metastasis.
Abstract Hypoxia induces a vast array of long non-coding RNAs (lncRNAs) in breast cancer cells, but their biological functions remain largely unknown. Here we identified a hitherto uncharacterized hypoxia-induced lncRNA RAB11B-AS1 in breast cancer cells. RAB11B-AS1 is a natural lncRNA upregulated in human breast cancer and its expression is induced by hypoxia-inducible factor 2 (HIF-2), but not HIF-1, in response to hypoxia. RAB11B-AS1 enhanced the expression of angiogenic factors including VEGFA and ANGPTL4 in hypoxic breast cancer cells by increasing recruitment of RNA polymerase II. In line with increased angio...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Niu Y, Bao L, Chen Y, Wang C, Luo M, Zhang B, Zhou M, Wang JE, Fang YV, Kumar A, Xing C, Wang Y, Luo W Tags: Cancer Res Source Type: research

Long noncoding RNA DRAIC inhibits prostate cancer progression by interacting with IKK to inhibit NF- κB activation.
Long noncoding RNA DRAIC inhibits prostate cancer progression by interacting with IKK to inhibit NF-κB activation. Cancer Res. 2020 Jan 03;: Authors: Saha S, Kiran M, Kuscu C, Chatrath A, Wotton D, Mayo MW, Dutta A Abstract DRAIC is a 1.7 kb spliced long noncoding RNA downregulated in castration-resistant advanced prostate cancer. Decreased DRAIC expression predicts poor patient outcome in prostate and seven other cancers, while increased DRAIC represses growth of xenografted tumors. Here we show that cancers with decreased DRAIC expression have increased NF-κB target gene expression. DRAI...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Saha S, Kiran M, Kuscu C, Chatrath A, Wotton D, Mayo MW, Dutta A Tags: Cancer Res Source Type: research

Cryo-EM structure of a type I-F CRISPR RNA guided surveillance complex bound to transposition protein TniQ.
PMID: 31900425 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Li Z, Zhang H, Xiao R, Chang L Tags: Cell Res Source Type: research

An Anisotropic Microstructure Evolution in a Solid Oxide Fuel Cell Anode.
Abstract The presented research shows that the long-term operation of a solid oxide fuel cell can lead to substantial anisotropic changes in anode material. The morphology of microstructure in the investigated stack was observed before and after the aging test using electron nanotomography. The microstructural parameters were estimated based on the obtained digital representation of the anode microstructure. Anisotropy was discovered in two of the three phases that constitute the anode, namely nickel and pores. The third component of the anode, which is yttrium-stabilized zirconia, remains isotropic. The changes a...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Brus G, Iwai H, Szmyd JS Tags: Nanoscale Res Lett Source Type: research

A reduction of primary cilia but not hedgehog signaling disrupts morphogenesis in testicular organoids.
Abstract Most mammalian cells possess a single, non-motile primary cilium that plays an important role in mediating cellular signaling pathways, such as Hedgehog (Hh) signaling. Primary cilia are present on testicular somatic cells and demonstrate a temporal expression during development; however, their role in testicular morphogenesis is not well characterized. To investigate the role of primary cilia and Hh signaling in Sertoli cells on morphogenesis, we inhibited assembly of primary cilia through CRISPR Cas9-mediated gene editing of ODF2, a structural component of primary cilia and siRNA-mediated gene silencing...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Goldsmith TM, Sakib S, Webster D, Carlson DF, Van der Hoorn F, Dobrinski I Tags: Cell Tissue Res Source Type: research

The implications of hippocampal neurogenesis in adolescent rats after status epilepticus: a novel role of notch signaling pathway in regulating epileptogenesis.
Abstract Seizure-induced neurogenesis has a widely recognized pro-epileptogenic function. Given the critical role of Notch signaling during the maintenance and neurogenesis of neural stem cells, we hypothesized that Notch may affect epileptogenesis and its progression through its role in neurogenesis in the adolescent rat brain. We used the lithium-pilocarpine-induced epilepsy model in adolescent Sprague-Dawley rats in order to evaluate hippocampal neurogenesis and epileptogenesis following the onset of status epilepticus (SE). We used western blotting analyses and qPCR to measure levels of Notch signaling at diff...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Yuan P, Han W, Xie L, Cheng L, Chen H, Chen J, Jiang L Tags: Cell Tissue Res Source Type: research

Histological differentiation of mucus cell subtypes suggests functional compartmentation in the eel esophagus.
Abstract We investigated the morphological and histological changes in eel esophagus during the course of freshwater (FW) to seawater (SW) transfer and identified multiple types of mucus cells from tissues that were fixed using Carnoy's solution to retain the mucus structure. The FW esophageal epithelium is stratified and composed of superficial cells, mucus cells, club cells (exocrine cells with a large vacuole), and basal cells. Two types of periodic acid-Schiff (PAS)-positive mucus cells were identified, and they can be further distinguished by the periodic acid-thionin Schiff/KOH/PAS (PAT) method, indicating t...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Wong MK, Uchida M, Tsukada T Tags: Cell Tissue Res Source Type: research

Combining the lack of chromogranins with chronic L-DOPA treatment affects motor activity in mice.
hado JD, Borges R Abstract We have tested whether the lack of chromogranins (Cgs) A and B could provoke CNS disorders when combined with an excess of dopamine. We chronically treated (over 6 months) mice lacking both chromogranins A and B (Cgs-KO) with a low oral dosage of L-DOPA/benserazide (10/2.5 mg/kg). Motor performance in the rota-rod test, open field activity, and metabolic cages indicated a progressive impairment in motor coordination in these mice, and an increase in rearing behavior, which was accompanied by an increase in DA within the substantia nigra. We conclude that mild chronic L-DOPA tre...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Castañeyra-Ruiz L, Castañeyra A, González-Santana A, Machado JD, Borges R Tags: Cell Tissue Res Source Type: research

Offloading items from memory: individual differences in cognitive offloading in a short-term memory task.
Abstract Cognitive offloading refers to the act of reducing the mental processing requirements of a task through physical actions like writing down information or storing information on a cell phone or computer. Offloading can lead to improved performance on ongoing tasks with high cognitive demand, such as tasks where multiple pieces of information must be simultaneously maintained. However, less is known about why some individuals choose to engage in offloading and under what conditions they might choose to do so. In the present study, offloading behavior is investigated in a short-term memory task requiring mem...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Morrison AB, Richmond LL Tags: Cogn Res Princ Implic Source Type: research

Retained or altered expression of major histocompatibility complex class I in patient-derived xenograft models in breast cancer.
Abstract The expression of major histocompatibility complex class I (MHC I) in tumor cells is regulated by interferon signaling, and it is an important factor in the efficacy of cytotoxic T cell-dependent immunotherapy. To determine the impact of immune cells in MHC I expression on tumor cells, we compared the expression of MHC I in tumor cells derived from primary breast cancers and patient-derived xenograft (PDX) models. MHC I and myxovirus resistance gene A (MxA) expression were analyzed using immunohistochemistry in 23 cases of tumor tissue and corresponding primary and secondary PDXs. The median H score of MH...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Song IH, Kim YA, Lee H, Park HS, Park IA, Sim CK, Lee MS, Gong G, Lee HJ Tags: Immunol Res Source Type: research

Meroterpenoid-Rich Fraction of the Ethanol Extract of Sargassum Serratifolium Suppresses Collagen-Induced Rheumatoid Arthritis in DBA/1J Mice Via Inhibition of Nuclear Factor κB Activation.
CONCLUSIONS: MES supplementation remarkably reduced inflammatory response in CIA mouse model. These results indicate that MES could be used as a pharmaceutical agent against RA. This article is protected by copyright. All rights reserved. PMID: 31900972 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Joung EJ, Kwon M, Gwon WG, Cao L, Lee SG, Utsuki T, Wakamatsu N, Kim JI, Kim HR Tags: Mol Nutr Food Res Source Type: research

Fluorescent fibre-optic confocal characterization of in vivo epidermal changes in atopic eczema.
CONCLUSIONS: Fibre-optic confocal imaging can visualize essential epidermal structures of atopic eczema directly in vivo, in real-time, and with cellular resolution thus without disturbing the natural state of the skin. FOCI is primarily a research tool, but with a potential to become used in the clinic for non-invasive microscopic diagnosis of AE and monitoring of effect of therapies. PMID: 31900995 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Suihko C, Serup J Tags: Skin Res Technol Source Type: research

Ultrastructure of the uterus, embryonic envelopes and the coracidium of the enigmatic tapeworm Tetracampos ciliotheca (Cestoda: Bothriocephalidea) from African sharptooth catfish (Clarias gariepinus).
Abstract Transmission electron microscopy (TEM) was used to study the ultrastructure of the uterus and egg morphology in the enigmatic bothriocephalidean tapeworm Tetracampos ciliotheca. The uterine wall, underlain by well-developed muscle bundles, consists of a syncytial epithelium which is characterized by the abundance of free ribosomes, mitochondria and cisternae of granular endoplasmic reticulum (GER). On the apical surface of the uterine epithelium, there is an abundant network of cytoplasmic microlamellae projecting into the uterine lumen. The lumen is filled with freely lying eggs which are located close t...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Yoneva A, Kuchta R, Smit N Tags: Parasitol Res Source Type: research

Potential of mesenchymal stem cells for bioengineered blood vessels in comparison with other eligible cell sources.
In conclusion, MSCs represent a preference in making autologous tissue-engineered vascular grafts (TEVGs) as well as off-the-shelf TEVGs for emergency vascular surgery cases. PMID: 31897835 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Afra S, Matin MM Tags: Cell Tissue Res Source Type: research

Professor Werner Franke, an inspired and inspiring personality. Forever young, forever friends.
PMID: 31897836 [PubMed - as supplied by publisher] (Source: Cell Research)
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Simionescu M Tags: Cell Tissue Res Source Type: research

Deciphering the response of asymmetry in the hydrophobic chains of novel cationic lipids towards biological function.
Abstract Cationic liposomes, a type of non-viral vectors, often play the important biological function of delivering nucleic acids during cell transfection. Variations in the molecular architecture of di-alkyl dihydroxy ethyl ammonium chloride-based cationic lipids involving hydrophobic tails have been found to influence their biological function in terms of cell transfection efficiency. For example, liposomes based on a cationic lipid (Lip1814) with asymmetry in the hydrophobic chains were found to display higher transfection efficacy in cultured mammalian cell lines than those comprising of symmetric Lip1818 or ...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Mukherjee D, Singh P, Rakshit T, Puthiya-Purayil TP, Vemula PK, Sengupta J, Das R, Pal SK Tags: Phys Chem Chem Phys Source Type: research

Ectopic expression of a cell-wall degrading enzyme induced OsAP2/ERF152, leads to resistance against bacterial and fungal infection in Arabidopsis.
Abstract Pathogen secreted cell wall degrading enzymes (CWDEs) induce plant innate immune responses. The expression of rice transcription factor, APETALA2/Ethylene response factor-152 (OsAP2/ERF152) is enhanced in leaves upon treatment with different CWDEs and upon wounding. Ectopic expression of OsAP2/ERF152 in Arabidopsis leads to induction of immune responses like callose deposition and upregulation of both salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) responsive defense genes. Arabidopsis transgenics expressing OsAP2/ERF152 exhibited resistance to infections caused by both bacterial and fungal pathoge...
Source: Cell Research - January 3, 2020 Category: Cytology Authors: Pillai SE, Kumar C, Dasgupta M, Kumar BK, Vungarala S, Kumar Patel H, Sonti R Tags: Phytopathology Source Type: research

Cell Res; +137 new citations
137 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2020/01/02PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - January 2, 2020 Category: Cytology Tags: Report Source Type: research

Cell Res; +162 new citations
162 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Cell Res These pubmed results were generated on 2019/12/31PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Cell Research)
Source: Cell Research - January 1, 2020 Category: Cytology Tags: Report Source Type: research

Comparative analysis of whey proteins in donkey colostrum and mature milk using quantitative proteomics.
In this study, we carried out qualitative and quantitative analysis of the donkey whey proteome using a label-free proteomic approach, combined with parallel reaction monitoring (PRM) as a validation method. A total of 300 whey proteins were identified in donkey colostrum (DC) and donkey mature (DM) milk, of which 18 were differentially expressed (P 
Source: Cell Research - December 30, 2019 Category: Cytology Authors: Li W, Li M, Cao X, Han H, Kong F, Yue X Tags: Food Res Int Source Type: research