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Correlation between S100A11 and the TGF- β1/SMAD4 pathway and its effects on the proliferation and apoptosis of pancreatic cancer cell line PANC-1.
Correlation between S100A11 and the TGF-β1/SMAD4 pathway and its effects on the proliferation and apoptosis of pancreatic cancer cell line PANC-1. Mol Cell Biochem. 2018 Jun 19;: Authors: Ji YF, Li T, Jiang F, Ni WK, Guan CQ, Liu ZX, Lu CH, Ni RZ, Wu W, Xiao MB Abstract S100A11 as a S100 protein family member has been documented to play dual-direction regulation over cancer cell proliferation. We explored the role of S100A11 in the proliferation and apoptosis of pancreatic cancer cell line PANC-1 and the potential mechanisms involving the TGF-β1/SMAD4/p21 pathway. S100A11 and TGF-β1 protein express...
Source: Molecular and Cellular Biochemistry - June 19, 2018 Category: Biochemistry Authors: Ji YF, Li T, Jiang F, Ni WK, Guan CQ, Liu ZX, Lu CH, Ni RZ, Wu W, Xiao MB Tags: Mol Cell Biochem Source Type: research
fMLP-dependent activation of Akt and ERK1/2 through ROS/Rho A pathways is mediated through restricted activation of the FPRL1 (FPR2) receptor
ConclusionCollectively, these data suggested that fMLP-activated ERK1/2 and Akt pathways through specific activation of the FPRL1/ROS/RoA-GTPase pathway.
Source: Inflammation Research - June 19, 2018 Category: Research Source Type: research
Genome-wide profiling of adult human papillary and reticular fibroblasts identifies ACAN, Col XI α1, and PSG1 as general biomarkers of dermis ageing, and KANK4 as an exemplary effector of papillary fibroblast ageing, related to contractility
Publication date: Available online 18 June 2018 Source:Mechanisms of Ageing and Development Author(s): Valérie Haydont, Véronique Neiveyans, Hélène Zucchi, Nicolas O. Fortunel, Daniel Asselineau Deciphering the characteristics of dermal fibroblasts is critical to further understand skin ageing. We have conducted a genome-wide transcriptomic characterization of papillary (Fp) and reticular (Fr) fibroblasts extracted from human skin samples corresponding to younger and older adult ages. From this screen, biomarkers suitable for the assessment of chronological ageing were identified, and extrapolated to the context of ph...
Source: Mechanisms of Ageing and Development - June 19, 2018 Category: Geriatrics Source Type: research
Thymoquinone Attenuates Myocardial Ischemia/Reperfusion Injury Through Activation of SIRT1 Signaling
Conclusions: The findings in this study demonstrate that TQ is efficient in attenuating MI/R injury through activation of the SIRT1 signaling pathway, which can thus reduce mitochondrial oxidative stress damage and cardiomyocyte apoptosis.Cell Physiol Biochem 2018;47:1193 –1206
Source: Cellular Physiology and Biochemistry - June 18, 2018 Category: Cytology Source Type: research
GSE115909 Differentially expressed genes after siRNA transfection in human cancer cell lines
Contributors : Naohiko Seki ; Yasutaka YamadaSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo identify differentially expressed genes by anti cancer treatments (siRNAs) in human cancer, several cell lines (lung cancer, breast cancer and pancreatic cancer) were subjected to Agilent whole genome microarrays.
Source: GEO: Gene Expression Omnibus - June 18, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
Lipidation of polyethylenimine-based polyplex increases serum stability of bioengineered RNAi agents and offers more consistent tumoral gene knockdown in vivo
Publication date: 25 August 2018 Source:International Journal of Pharmaceutics, Volume 547, Issues 1–2 Author(s): Qian-Yu Zhang, Pui Yan Ho, Mei-Juan Tu, Joseph L. Jilek, Qiu-Xia Chen, Su Zeng, Ai-Ming Yu Recently we have established a novel approach to produce bioengineered noncoding RNA agents (BERAs) in living cells that carry target RNAi molecules (e.g., siRNA and miRNA) and thus act as “prodrugs”. Using GFP-siRNA-loaded BERA (BERA/GFP-siRNA) as a model molecule, this study was to define the in vitro and in vivo knockdown efficiency of BERAs delivered by liposome-polyethylenimine nanocomplex (lipopolyplex or LPP...
Source: International Journal of Pharmaceutics - June 18, 2018 Category: Drugs & Pharmacology Source Type: research
Knockdown of TRIM66 inhibits malignant behavior and epithelial-mesenchymal transition in non-small cell lung cancer.
CONCLUSION: Silence TRIM66 expression suppressed NSCLC cell proliferation, invasion, and migration. The siRNA-mediated TRIM66 silencing could block the occurrence of EMT. TRIM66 could be a promising novel target for future NSCLC treatments. PMID: 29929749 [PubMed - as supplied by publisher]
Source: Pathology, Research and Practice - June 18, 2018 Category: Pathology Authors: Dai HY, Ma Y, Da Z, Hou XM Tags: Pathol Res Pract Source Type: research
Silencing of LncRNA BDNF-AS attenuates A β25-35-induced neurotoxicity in PC12 cells by suppressing cell apoptosis and oxidative stress.
CONCLUSION: Silencing BDNF-AS exerts protective functions to increase the viability, inhibit the apoptosis and oxidative stress of Aβ25-35-induced PC12 cells by negative regulation of BDNF. ABBREVIATIONS: Aβ25-35: amyloid beta peptide 25-35; AD: Alzheimer's disease; LncRNA BDNF-AS: long non-coding RNA brain-derived neurotrophic factor anti-sense; OS: Oxidative stress. PMID: 29902125 [PubMed - as supplied by publisher]
Source: Neurological Research - June 16, 2018 Category: Neurology Tags: Neurol Res Source Type: research
DNA binding protein HMGB1 secreted by activated microglia promotes the apoptosis of hippocampal neurons in diabetes complicated with OSA.
Abstract Type 2 diabetes mellitus (T2DM) complicated with obstructive sleep apnea (OSA) may cause neuronal apoptosis and cognitive deficits, but the underlying mechanisms remain unclear. We aimed to determine the relationship between the activation of microglia and the apoptosis of hippocampal neurons, specifically in terms of high mobility group box-1 (HMGB1), after high glucose (HG) and intermittent hypoxia (IH) exposure. Diabetic KK-Ay mice and non-diabetic C57BL/6J mice (C57 mice) underwent IH or normoxia (control) exposure for 4 weeks. Cognitive function, microglial activation and hippocampal neuronal apoptos...
Source: Brain, Behavior, and Immunity - June 16, 2018 Category: Neurology Authors: Shi Y, Guo X, Zhang J, Zhou H, Sun B, Feng J Tags: Brain Behav Immun Source Type: research
Lipopolysaccharide induced the proliferation of mouse lung fibroblasts by suppressing FoxO3a/p27 pathway.
Abstract Aberrant aggregation and activation of lung fibroblasts is a key process in pulmonary fibrosis, but the underlying mechanism remains enigmatic. Forkhead Box O3a (FoxO3a) is considered to be an important transcription factor that could regulate both cell cycle and cell viability. To investigate the role of FoxO3a on LPS-induced lung fibroblast proliferation, we transfected FoxO3a-SiRNA or FoxO3a-OE lentivirus into cultured mouse lung fibroblasts to knockdown or overexpress FoxO3a and pretreated mouse lung fibroblasts with gefitinib to enhance FoxO3a activity. The proliferation of lung fibroblasts was evalu...
Source: Cell Biology International - June 16, 2018 Category: Cytology Authors: Gu N, Xing S, Chen S, Zhou Y, Jiang T, Jiao Y, Gao Y, Yu W, He Z, Wen D Tags: Cell Biol Int Source Type: research
ETS1 regulates Twist1 transcription in a Kras G12D / Lkb1 −/− metastatic lung tumor model of non-small cell lung cancer
AbstractDistinct members of the Ets family of transcription factors act as positive or negative regulators of genes involved in cellular proliferation, development, and tumorigenesis. In human lung cancer, increased ETS1 expression is associated with poor prognosis and metastasis. We tested whether ETS1 contributes to lung tumorigenesis by binding to Twist1, a gene involved in tumor cell motility and dissemination. We used a mouse lung cancer model with metastasis driven by conditionally activatedKras and concurrent tumor suppressorLkb1 loss (KrasG12D/Lkb1−/− model) and a similar model of lung cancer that does not meta...
Source: Clinical and Experimental Metastasis - June 16, 2018 Category: Cancer & Oncology Source Type: research
Girdin regulates the proliferation and apoptosis of pancreatic cancer cells via the PI3K/Akt signalling pathway.
Authors: Wang S, Lei Y, Cai Z, Ye X, Li L, Luo X, Yu C Abstract Girdin functions as an Akt phosphorylation enhancer (APE), which expedites the proliferation and survival of many types of tumours. However, the influence of Girdin on pancreatic cancer and the underlying molecular mechanisms have yet to be uncovered. Hence, in the present study, we sought to elucidate the function of Girdin in pancreatic cancer malignancy, particularly its role in pancreatic cancer cell proliferation, migration and apoptosis. Immunohistochemistry (IHC) was used to evaluate Girdin expression in pancreatic cancer tissues and to analyse ...
Source: Oncology Reports - June 15, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Anion exchanger 2 suppresses cellular movement and has prognostic significance in esophageal squamous cell carcinoma.
Conclusions: The results of the present study suggest that reductions in AE2 in ESCC enhance cellular movement by activating MMP signaling pathways and are related to a poor prognosis in patients with ESCC. Methods: In human ESCC cell lines, knockdown experiments were conducted using AE2 siRNA, and the effects on cellular movement and survival were analyzed. The gene expression profiles of cells were examined using a microarray analysis. An immunohistochemical analysis was performed on 61 primary tumor samples obtained from ESCC patients who underwent esophagectomy. PMID: 29899837 [PubMed]
Source: Oncotarget - June 15, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Synthetic lethality in CCNE1-amplified high grade serous ovarian cancer through combined inhibition of Polo-like kinase 1 and microtubule dynamics.
In this study, we conducted an siRNA-based kinome screen to identify modulators of mitotic progression in CCNE1-amplified HGSOC cells that may influence clinical paclitaxel response. PLK1 is overexpressed in many types of cancer, which correlates with poor prognosis. Here, we identified a novel synthetic lethal interaction of the clinical PLK1 inhibitor BI6727 and the microtubule-targeting drug paclitaxel in HGSOC cell lines with CCNE1-amplification and elucidated the underlying molecular mechanisms of this synergism. BI6727 synergistically induces apoptosis together with paclitaxel in different cell lines including a pati...
Source: Oncotarget - June 15, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Spleen tyrosine kinase promotes NLR family pyrin domain containing 3 inflammasome ‑mediated IL‑1β secretion via c‑Jun N‑terminal kinase activation and cell apoptosis during diabetic nephropathy.
Spleen tyrosine kinase promotes NLR family pyrin domain containing 3 inflammasome‑mediated IL‑1β secretion via c‑Jun N‑terminal kinase activation and cell apoptosis during diabetic nephropathy. Mol Med Rep. 2018 Jun 14;: Authors: Qiao Y, Tian X, Men L, Li S, Chen Y, Xue M, Hu Y, Zhou P, Long G, Shi Y, Liu R, Liu Y, Qi Z, Cui Y, Shen Y Abstract Diabetic nephropathy (DN) is a serious complication of diabetes and can cause an increased mortality risk. It was previously reported that NLR family pyrin domain containing 3 (NLRP3) inflammasome is involved in the pathogenesis of diabetes. However, the...
Source: Molecular Medicine Reports - June 15, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
B7 ‑H1‑mediated immunosuppressive properties in human mesenchymal stem cells are mediated by STAT‑1 and not PI3K/Akt signaling.
B7‑H1‑mediated immunosuppressive properties in human mesenchymal stem cells are mediated by STAT‑1 and not PI3K/Akt signaling. Mol Med Rep. 2018 May 30;: Authors: Jang IK, Jung HJ, Noh OK, Lee DH, Lee KC, Park JE Abstract Mesenchymal stem cells (MSCs), derived from either bone marrow (BM) or Wharton's jelly (WJ), inhibit the proliferation of activated T cells, and interferon (IFN)‑γ serves an important role in this process. This process is B7‑homolog (H)1‑dependent during cell contact inhibition. However, the signaling pathway involved in B7‑H1 expression in MSCs remains largely undefine...
Source: Molecular Medicine Reports - June 15, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
GSE115801 Differentially expressed genes after miRNA or siRNA transfection in human cancer cell lines III
Contributors : Naohiko Seki ; Takayuki AraiSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo identify differentially expressed genes by anti cancer treatments (microRNAs or siRNAs) in human cancer, several cell lines ( pancreatic cancer, renal cell carcinoma, oral squamous cell carcinoma, prostate cancer and bladder cancer) were subjected to Agilent whole genome microarrays.
Source: GEO: Gene Expression Omnibus - June 15, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
ASAP Biodegradable Black Phosphorus Nanosheets Mediate Specific Delivery of hTERT siRNA for Synergistic Cancer Therapy
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b04807
Source: ACS Applied Materials and Interfaces - June 15, 2018 Category: Materials Science Authors: Lei Chen, Chuan Chen, Wei Chen, Ke Li, Xiaozhen Chen, Xudong Tang, Ganfeng Xie, Xi Luo, Xiaojiao Wang, Houjie Liang, Songtao Yu Source Type: research
Formononetin inhibits neuroinflammation and increases estrogen receptor beta (ER β) protein expression in BV2 microglia.
This study has established that formononetin inhibits neuroinflammation by targeting NF-κB signalling pathway in BV2 microglia, possibly through mechanisms involving ERβ. Formononetin appears to modulate ERβ in MCF7 breast cancer cells with limited proliferative effect. Formononetin could therefore serve as a chemical scaffold for the development of novel compounds which have selective neuroprotective actions in the brain. PMID: 29913427 [PubMed - as supplied by publisher]
Source: International Immunopharmacology - June 15, 2018 Category: Allergy & Immunology Authors: El-Bakoush A, Olajide OA Tags: Int Immunopharmacol Source Type: research
HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis
High-mobility group box-1 (HMGB1) plays a context-dependent role in autophagy, which is required for hepatic stellate cells (HSCs) activation. However, the significance of HMGB1-induced HSCs autophagy in liver fibrosis has not been elucidated. Here, we first documented an enrichment of peripheral and intra-hepatic HMGB1 signal in hepatitis B virus (HBV)-related liver fibrosis progression, and presented the direct evidence of anatomic proximity of HMGB1 with a-SMA (a marker for HSCs activation) in cirrhotic liver specimens. Then, we demonstrated the autophagy-inducing effects by serum-sourced HMGB1 in both primary murine HS...
Source: Clinical Science - June 15, 2018 Category: Biomedical Science Authors: Li, J., Zeng, C., Zheng, B., Liu, C., Tang, M., Jiang, Y., Chang, Y., Song, W., Wang, Y., Yang, C. Tags: PublishAheadOfPrint Source Type: research
Expression of AGPAT2, an enzyme involved in the glycerophospholipid/triacylglycerol biosynthesis pathway, is directly regulated by HIF-1 and promotes survival and etoposide resistance of cancer cells under hypoxia
In conclusion, our findings demonstrate that AGPAT2, which is mutated in patients with congenital generalized lipodystrophy and over-expressed in different types of cancer, is a direct transcriptional target of HIF-1, suggesting that upregulation of lipid storage by HIF-1 plays an important role in adaptation and survival of cancer cells under low oxygen conditions. Graphical abstract
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - June 15, 2018 Category: Lipidology Source Type: research
Sterol carrier protein-2 deficiency attenuates diet-induced dyslipidemia and atherosclerosis in mice Metabolism
Intracellular cholesterol transport proteins move cholesterol to different subcellular compartments and thereby regulate its final metabolic fate. In hepatocytes, for example, delivery of high-density lipoprotein (HDL)–associated cholesterol for bile acid synthesis or secretion into bile facilitates cholesterol elimination from the body (anti-atherogenic effect), whereas delivery for esterification and subsequent incorporation into apolipoprotein B–containing atherogenic lipoproteins (e.g. very-low-density lipoprotein (VLDL)) enhances cholesterol secretion into the systemic circulation (pro-atherogenic effect). Intrace...
Source: Journal of Biological Chemistry - June 15, 2018 Category: Chemistry Authors: Hongliang He, Jing Wang, Paul J. Yannie, Genta Kakiyama, William J. Korzun, Shobha Ghosh Tags: Lipids Source Type: research
Role of the NRP-1-mediated VEGFR2-independent pathway on radiation sensitivity of non-small cell lung cancer cells
ConclusionsWe demonstrated that when VEGFR2 was inhibited, NRP-1 appeared to regulate RAD51 expression through the VEGFR2-independent ABL-1 pathway, consequently regulating radiation sensitivity. In addition, the combined inhibition of VEGFR2 and NRP-1 appears to sensitize cancer cells to radiation.
Source: Journal of Cancer Research and Clinical Oncology - June 14, 2018 Category: Cancer & Oncology Source Type: research
Selective delivery of PLXDC1 small interfering RNA to endothelial cells for anti-angiogenesis tumor therapy using CD44-targeted chitosan nanoparticles for epithelial ovarian cancer.
This study aimed to improve targeted delivery and enhance therapeutic efficacy for tumor anti-angiogenesis. The HA-CH-NP/siRNA was 200 ± 10 nm in size with a zeta potential of 26.4 mV. The loading efficiency of siRNA to the HA-CH-NP/siRNA was up to 60%. The selective binding of HA-CH-NP/siRNA to CD44-positive tumor endothelial cells increased by 2.1-fold compared with that of the CD44 nontargeted CH-NP/siRNA. PLXDC1 silencing by the HA-CH-NP/siRNA significantly inhibited tumor growth in A2780 tumor-bearing mice compared with that in the control group (p < .01), and mRNA expression of PLXDC1 was significantl...
Source: Drug Delivery - June 14, 2018 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research
MicroRNA-499a decelerates glioma cell proliferation while accelerating apoptosis through the suppression of Notch1 and the MAPK signaling pathway.
Abstract As the most common and lethal of intracranial tumors, glioma accounts for 81% of all malignant brain tumors. Research data has identified the role of microRNAs (miRs) as functional suppressers in the progression of Glioma. The present study aimed to, ascertain as to whether microRNA-499a (miR-499a) influences cell proliferation and apoptosis through the MAPK signaling pathway by targeting Notch1 in glioma. Both glioma and adjacent tissues between 2012~2016, were obtained from People's Hospital of Zhengzhou University (Henan Provincial People's Hospital). The collected glioma cells were treated with miR-44...
Source: Brain Research Bulletin - June 14, 2018 Category: Neurology Authors: Wang BQ, Yang B, Yang HC, Wang JY, Hu S, Gao YS, Bu XY Tags: Brain Res Bull Source Type: research
SNAP23 suppresses cervical cancer progression via modulating the cell cycle.
CONCLUSIONS: Our results revealed that SNAP23 suppressed progression of CC and induced cell cycle G2/M arrest via upregulating p21cip1 and downregulating CyclinB1. PMID: 29908998 [PubMed - as supplied by publisher]
Source: Gene - June 14, 2018 Category: Genetics & Stem Cells Authors: Zhu B, Zhang Q, Wu Y, Luo J, Zheng X, Xu L, Lu E, Qu J, Ren B Tags: Gene Source Type: research
In vitro inhibitory properties of sesquiterpenes from Chloranthus serratus on cell motility via down-regulation of LIMK1 activation in human breast cancer
Conclusion collectively, it was concluded that SERA exhibited significant inhibitory effects on breast cancer cells migration, and these effects of this sesquiterpene are due to its properties reducing the activation of LIM kinase 1. Graphical abstract
Source: Phytomedicine - June 14, 2018 Category: Drugs & Pharmacology Source Type: research
Expression of AGPAT2, an enzyme involved in the glycerophospholipid/triacylglycerol biosynthesis pathway, is directly regulated by HIF-1 and promotes survival and etoposide resistance of cancer cells under hypoxia.
In conclusion, our findings demonstrate that AGPAT2, which is mutated in patients with congenital generalized lipodystrophy and over-expressed in different types of cancer, is a direct transcriptional target of HIF-1, suggesting that upregulation of lipid storage by HIF-1 plays an important role in adaptation and survival of cancer cells under low oxygen conditions. PMID: 29908837 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - June 14, 2018 Category: Biochemistry Authors: Triantafyllou EA, Georgatsou E, Mylonis I, Simos G, Paraskeva E Tags: Biochim Biophys Acta Source Type: research
Induction of REDD1 via AP-1 prevents oxidative stress-mediated injury in hepatocytes.
Abstract Regulated in development and DNA damage responses 1 (REDD1) is an inducible gene in response to various stresses, which functions as a negative regulator of the mammalian target of rapamycin protein kinase in complex 1. In the present study, we identified the role of REDD1 under the oxidative stress-mediated hepatocyte injury and its regulatory mechanism. REDD1 protein was increased in H2O2 or tert-butylhydroperoxide (t-BHP)-treated hepatocytes. H2O2 also elevated REDD1 mRNA levels. This event was inhibited by antioxidants such as diphenyleneiodonium chloride, N-acetyl-L-cysteine, or butylated hydroxy ani...
Source: Free Radical Biology and Medicine - June 14, 2018 Category: Biology Authors: Cho SS, Kim KM, Yang JH, Kim JY, Park SJ, Kim SJ, Kim JK, Cho IJ, Ki SH Tags: Free Radic Biol Med Source Type: research
Improving Senescent Wound Healing With Local and Systemic Therapies
The population is aging, and the prevalence of chronic wounds is increasing. Because neovascularization is essential for tissue repair and both local and systemic factors affect new blood vessel formation, we hypothesize that altering either pathway would reciprocally enhance wound healing in the aged. To test this hypothesis, p53 was locally suppressed and endothelial progenitor cells (EPCs) were systemically mobilized in a murine model of senescent wound healing. Bilateral 6-mm full-thickness stented wounds were made on the dorsum of Zmpste24−/− mice. Animals received weekly topical p53 small interfering RNA (siRNA)...
Source: Annals of Plastic Surgery - June 13, 2018 Category: Cosmetic Surgery Tags: Transplantation Surgery and Research Source Type: research
Long Noncoding RNA (lncRNA) n379519 Promotes Cardiac Fibrosis in Post-Infarct Myocardium by Targeting miR-30.
CONCLUSIONS These findings reveal a novel function of n379519-miR-30 axis as a negative regulator for the treatment of MI-induced cardiac fibrosis and the associated cardiac dysfunction. PMID: 29889825 [PubMed - in process]
Source: Medical Science Monitor - June 13, 2018 Category: Research Tags: Med Sci Monit Source Type: research
ASAP Overcoming Multidrug Resistance by Codelivery of MDR1-Targeting siRNA and Doxorubicin Using EphA10-Mediated pH-Sensitive Lipoplexes: In Vitro and In Vivo Evaluation
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b01806
Source: ACS Applied Materials and Interfaces - June 13, 2018 Category: Materials Science Authors: Jiulong Zhang, Zhouqi Du, Shuang Pan, Menghao Shi, Jie Li, Chunrong Yang, Haiyang Hu, Mingxi Qiao, Dawei Chen, Xiuli Zhao Source Type: research
A2BAR activation attenuates acute lung injury by inhibiting alveolar epithelial cell apoptosis both in vivo and in vitro.
In conclusion, A2BAR activation effectively attenuated OA-induced ALI by inhibiting AEC apoptosis and mitigated H2O2-induced AEC injury by suppressing the p38 and ERK1/2-mediated mitochondrial apoptosis pathway. PMID: 29898376 [PubMed - as supplied by publisher]
Source: Am J Physiol Cell Ph... - June 13, 2018 Category: Cytology Authors: Xu X, Zhu Q, Niu F, Zhang R, Wang Y, Wang W, Sun D, Wang X, Wang A Tags: Am J Physiol Cell Physiol Source Type: research
Dual gain and loss of cullin 3 function mediates familial hyperkalemic hypertension.
In conclusion, deletion of exon 9 from Cul3 generates a protein that is itself ubiquitin ligase-defective, but also capable of enhanced autophagocytic KLHL3 degradation, thereby exerting dominant-negative effects on the WT-allele. PMID: 29897280 [PubMed - as supplied by publisher]
Source: Am J Physiol Renal P... - June 13, 2018 Category: Urology & Nephrology Authors: Cornelius RJ, Zhang C, Erspamer KJ, Agbor LN, Sigmund CD, Singer JD, Yang CL, Ellison DH Tags: Am J Physiol Renal Physiol Source Type: research
Deubiquitinase USP48 promotes ATRA-induced granulocytic differentiation of acute promyelocytic leukemia cells.
In conclusion, the present study highlights the function of USP48 in the ATRA-induced granulocytic differentiation of APL cells and provides a theoretical basis for identifying novel targets for differentiation therapy of APL. PMID: 29901102 [PubMed - as supplied by publisher]
Source: International Journal of Oncology - June 13, 2018 Category: Cancer & Oncology Authors: Li L, Wang Y, Zhang X, Song G, Guo Q, Zhang Z, Diao Y, Yin H, Liu H, Jiang G Tags: Int J Oncol Source Type: research
LTBP2 knockdown and oxidative stress affect glaucoma features including TGF β pathways, ECM genes expression and apoptosis in trabecular meshwork cells.
LTBP2 knockdown and oxidative stress affect glaucoma features including TGFβ pathways, ECM genes expression and apoptosis in trabecular meshwork cells. Gene. 2018 Jun 13;: Authors: Suri F, Yazdani S, Elahi E Abstract Glaucoma is the leading cause of irreversible blindness worldwide. Although the etiology of glaucoma is incompletely understood, it is known that the extracellular matrix (ECM) of the trabecular meshwork, oxidative stress, TGFβ signaling pathways, and apoptosis are important components of glaucoma pathogenesis. These components appear to be interrelated, but knowledge on their interacti...
Source: Gene - June 13, 2018 Category: Genetics & Stem Cells Authors: Suri F, Yazdani S, Elahi E Tags: Gene Source Type: research
Effects of the stimuli-dependent enrichment of 8-oxoguanine DNA glycosylase1 on chromatinized DNA
Publication date: Available online 12 June 2018 Source:Redox Biology Author(s): Wenjing Hao, Tianyang Qi, Lang Pan, Ruoxi Wang, Bing Zhu, Leopoldo Aguilera-Aguirre, Zsolt Radak, Tapas K. Hazra, Spiros A. Vlahopoulos, Attila Bacsi, Allan R. Brasier, Xueqing Ba, Istvan Boldogh 8-Oxoguanine DNA glycosylase1 (OGG1) initiates the base excision repair pathway by removing one of the most abundant DNA lesions, 8-oxo-7,8-dihydroguanine (8-oxoG). Recent data showed that 8-oxoG not only is a pro-mutagenic genomic base lesion, but also functions as an epigenetic mark and that consequently OGG1 acquire distinct roles in modulation of ...
Source: Redox Biology - June 12, 2018 Category: Biology Source Type: research
RNA interference targeting CD147 inhibits metastasis and invasion of human breast cancer MCF-7 cells by downregulating MMP-9/VEGF expression.
In this study, we investigated the role of CD147 in human breast cancer metastasis and invasion, and examined its underlying molecular mechanisms. Immunohistochemistry results revealed high expression of CD147 in human breast tumor tissues, which was positively correlated with the malignancy of breast cancer. MCF-7 cells were transfected with CD147 siRNA eukaryotic expression vector, which resulted in significant knockdown of CD147. We found that CD147 siRNA dramatically inhibited cell proliferation, metastasis, and invasion. Furthermore, our results demonstrated that CD147 siRNA inhibited the synthesis of matrix metallopr...
Source: Acta Biochimica et Biophysica Sinica - June 12, 2018 Category: Biochemistry Authors: Li F, Zhang J, Guo J, Jia Y, Han Y, Wang Z Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research
Elafin treatment rescues EGFR-Klf4 signaling and lung cell survival in ventilated newborn mice.
CONCLUSIONS: EGFR-Klf4 is a novel pro-survival signaling pathway in lung that MV disrupts. Elafin preserves pEGFR-Klf4 signaling and inhibits apoptosis, thereby enabling lung growth during MV. Our animal and human data together beg the question: would elastase inhibition prevent BPD in high-risk infants exposed to MV-O2? PMID: 29894205 [PubMed - as supplied by publisher]
Source: Am J Respir Cell Mol... - June 12, 2018 Category: Respiratory Medicine Authors: Alejandre Alcazar MA, Kaschwich M, Ertsey R, Preuss S, Milla C, Mujahid S, Masumi J, Khan S, Mokres LM, Tian L, Mohr J, Hirani DV, Rabinovitch M, Bland RD Tags: Am J Respir Cell Mol Biol Source Type: research
CIP2A facilitates the G1/S cell cycle transition via B-Myb in human papillomavirus 16 oncoprotein E6-expressing cells.
In this study, we demonstrated that HPV-16E6 significantly up-regulated CIP2A mRNA and protein expression in a p53-degradation-dependent manner. Knockdown of CIP2A by siRNA inhibited viability and DNA synthesis and caused G1 cell cycle arrest of 16E6-expressing cells. Knockdown of CIP2A resulted in a significant reduction in the expression of cyclin-dependent kinase 1 (Cdk1) and Cdk2. Although CIP2A has been reported to stabilize c-Myc by inhibiting PP2A-mediated dephosphorylation of c-Myc, we have presented evidence that the regulation of Cdk1 and Cdk2 by CIP2A is dependent on transcription factor B-Myb rather than c-Myc....
Source: J Cell Mol Med - June 12, 2018 Category: Molecular Biology Authors: Tian Y, Chen H, Qiao L, Zhang W, Zheng J, Zhao W, Chen JJ, Zhang W Tags: J Cell Mol Med Source Type: research
Cancer-associated fibroblasts promote gastric tumorigenesis through EphA2 activation in a ligand-independent manner
ConclusionCAFs may contribute to gastric tumorigenesis by activating EphA2 signaling pathway in a ligand-independent manner. Our results suggest that ligand-independent activation of EphA2 was triggered by VEGF released from CAF-CM. Our result may partially explain why ligand-dependent tumor suppressor roles of EphA2 are not evident in gastric cancer despite the prominent level of ephrinA1.
Source: Journal of Cancer Research and Clinical Oncology - June 12, 2018 Category: Cancer & Oncology Source Type: research
HRAS as a potential therapeutic target of salirasib RAS inhibitor in bladder cancer.
Abstract The active form of the small GTPase RAS binds to downstream effectors to promote cell growth and proliferation. RAS signal enhancement contributes to tumorigenesis, invasion, and metastasis in various different cancers. HRAS proto-oncogene GTPase (HRAS), one of the RAS isoforms, was the first human oncogene for which mutations were reported in T24 bladder cancer (BC) cells in 1982, and HRAS mutation or upregulation has been reported in several cancers. According to data from The Cancer Genome Atlas, HRAS expression was significantly upregulated in clinical BC samples compared to healthy samples (P=0.0024...
Source: International Journal of Oncology - June 11, 2018 Category: Cancer & Oncology Authors: Sugita S, Enokida H, Yoshino H, Miyamoto K, Yonemori M, Sakaguchi T, Osako Y, Nakagawa M Tags: Int J Oncol Source Type: research
α2 isoform of Na+,K+-ATPase via Na+,Ca2+ exchanger modulates myelin basic protein synthesis in oligodendrocyte lineage cells in vitro.
Abstract Oligodendrocytes in the CNS myelinate neuronal axons, facilitating rapid propagation of action potentials. Myelin basic protein (MBP) is an essential component of myelin and its absence results in severe hypomyelination. In oligodendrocyte lineage cell (OLC) monocultures MBP synthesis starts at DIV4. Ouabain (10 nM), a Na+,K+-ATPase (NKA) blocker, stimulates MBP synthesis. As OLCs express the α2 isoform of NKA (α2-NKA) that has a high affinity for ouabain, we hypothesized that α2-NKA mediates this effect. Knockdown of α2-NKA with small interfering (si)RNA (α2-siRNA) significantly potentiated MBP sy...
Source: Cell Calcium - June 10, 2018 Category: Cytology Authors: Hammann J, Bassetti D, White R, Luhmann HJ, Kirischuk S Tags: Cell Calcium Source Type: research
Silicon dioxide nanoparticles induce COX-2 expression through activation of STAT3 signaling pathway in HaCaT cells.
Abstract Silicon dioxide nanoparticles (SiO2-NPs) are widely used in biomedicines and consumer products, such as sunscreens and cosmetics. However, SiO2-NPs can cause adverse effects on human health, depending on the size and concentration of nanoparticles. The present study was aimed at investigating the molecular mechanism underlying SiO2-NPs-induced inflammation in human keratinocyte (HaCaT) cells. Incubation of HaCaT cells with SiO2-NPs induced the expression of cyclooxygenase-2 (COX-2) mRNA and protein. Treatment of cells with SiO2-NPs also induced the phosphorylation, DNA binding and the reporter gene activi...
Source: Toxicology in Vitro - June 9, 2018 Category: Toxicology Authors: Kundu J, Kim DH, Chae IG, Lee JK, Lee S, Jeong CH, Chun KS Tags: Toxicol In Vitro Source Type: research
Targeted inhibition of Axl receptor tyrosine kinase ameliorates anti-GBM-induced lupus-like nephritis.
Abstract Glomerulonephritis (GN) is a typical lesion in autoantibody and immune complex disorders, including SLE. Because the Gas6/Axl pathway has been implicated in the pathogenesis of many types of GN, targeting this pathway might ameliorate GN. Consequently, we have studied the efficacy and mechanism of R428, a potent selective Axl inhibitor, in the prevention of experimental anti-GBM nephritis. Axl upregulation was investigated with Sp1/3 siRNA in the SV40-transformed mesangial cells. For Axl inhibition, a daily dose of R428 (125 mg/kg) or vehicle was administered orally. GN was induced with anti-GBM sera. R...
Source: Journal of Autoimmunity - June 9, 2018 Category: Allergy & Immunology Authors: Zhen Y, Lee IJ, Finkelman FD, Shao WH Tags: J Autoimmun Source Type: research
Caveolin-1 regulates autophagy activity in thyroid follicular cells and is involved in Hashimoto's thyroiditis disease.
In this study, we examined Th1 cytokines and Cav-1 expression in HT thyroid tissues, determined the effects of interleukin-1beta (IL-1β) and interferon-gamma (IFN-γ) on Cav-1 and autophagy activity in TFC, and investigated the association between Cav-1 and autophagy activity in vitro. Our results indicate that higher levels of IL-1β and IFN-γ and lower levels of Cav-1 were expressed in thyroid tissues of HT patients than in those of normal controls. Cav-1 mRNA and protein levels were significantly decreased in TFC exposed to IL-1β and IFN-γ, accompanied by decreased expression of autophagy-related protein LC3B-II. In...
Source: Endocrine Journal - June 8, 2018 Category: Endocrinology Tags: Endocr J Source Type: research
Transcriptional activation of p21Waf1 contributes to suppression of HR by p53 in response to replication arrest induced by camptothecin.
Authors: Romanova LY, Mushinski F, Kovalchuk AL Abstract The inhibitory effect of p53 on homologous recombination (HR) is exerted through sequestration of replication protein A (RPA). Release of the p53/RPA complex in response to replication stress is crucially dependent on the phosphorylation status of both proteins and is required for efficient DNA repair by HR. Phosphorylation of RPA within its RPA2 subunit by cyclin-dependent kinases (CDK) is an early event in the replication stress response. Here we investigated the role of transcriptional activation of the p53 downstream target, p21Waf1, on RPA2 phosphorylati...
Source: Oncotarget - June 8, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research
LncRNA FAS-AS1 promotes the degradation of extracellular matrix of cartilage in osteoarthritis.
CONCLUSIONS: The expression of FAS-AS1 was increased in osteoarthritis, and FAS-AS1 could be involved in the development of the disease by regulating the proliferation, apoptosis of chondrocytes and promoting the degradation of extracellular matrix. PMID: 29863238 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 8, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
Neuroprotection against 6-OHDA toxicity in PC12 cells and mice through the Nrf2 pathway by a sesquiterpenoid from Tussilago farfara
Publication date: September 2018 Source:Redox Biology, Volume 18 Author(s): Joohee Lee, Kwangho Song, Eugene Huh, Myung Sook Oh, Yeong Shik Kim Oxidative stress plays a key role in neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Therefore, the nuclear factor-E2-related factor 2 (Nrf2), a key regulator of the antioxidative response, is considered to be important as a therapeutic target for neurodegenerative diseases. We investigated the underlying mechanism of Nrf2-mediated neuroprotective effects against oxidative stress in the PC12 cell line by 7β-(3-ethyl-cis-crotonoyloxy)-1α-(2-methylbutyrylo...
Source: Redox Biology - June 8, 2018 Category: Biology Source Type: research
Suppression of Pax2 Attenuates Allodynia and Hyperalgesia through ET-1 –ETAR–NFAT5 Signaling in a Rat Model of Neuropathic Pain
Publication date: 1 August 2018 Source:Neuroscience, Volume 384 Author(s): Lydia Wai Tai, Zhiqiang Pan, Liting Sun, Haobo Li, Pan Gu, Stanley Sau Ching Wong, Sookja K. Chung, Chi Wai Cheung Endothelin-1 (ET-1) and its receptors (ETAR/ETBR) emerge to be a key signaling axis in neuropathic pain processing and are recognized as new therapeutic targets. Yet, little is known on the functional regulation of ET-1 axis during neuropathic pain. Bioinformatics analysis indicated that paired box gene 2 (Pax2) or nuclear factor of activated T-cells 5 (NFAT5), two transcription factors involved in the modulation of neurotransmission, ...
Source: Neuroscience - June 8, 2018 Category: Neuroscience Source Type: research