Query: siRNA

This page shows you your search results in order of date. This is page number 9.

Order by Relevance | Date

Total 14406 results found since Jan 2013.

ASAP Near-Infrared Photothermal Release of siRNA from the Surface of Colloidal Gold –Silver–Gold Core–Shell–Shell Nanoparticles Studied with Second-Harmonic Generation
The Journal of Physical Chemistry CDOI: 10.1021/acs.jpcc.8b06117
Source: The Journal of Physical Chemistry C - August 17, 2018 Category: Chemistry Authors: Raju R. Kumal, Mohammad Abu-Laban, Prakash Hamal, Blake Kruger, Holden T. Smith, Daniel J. Hayes, Louis H. Haber Source Type: research

GSE113369 CDK4 inhibition diminishes p53 activation by Mdm2-antagonists
Contributors : Matthias Dobbelstein ; Anusha SriramanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCDK4 inhibitors have reached clinical approval for cancer therapy. In parallel, the p53 antagonist Mdm2 remains an attractive target for anti-cancer therapy, including numerous clinical studies. The genes encoding Mdm2 and CDK4 are frequently co-amplified in human malignancies, most notably in liposarcomas, suggesting their combined targeting for therapy. Here we show, however, that small compounds that inhibit Mdm2 and CDK4 antagonize each other rather than synergize in their cytotoxi...
Source: GEO: Gene Expression Omnibus - August 17, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Cloning, expression analysis and functional characterization of an interleukin-1 receptor-associated kinase 4 from Apostichopus japonicus
In this study, an IRAK4 homologue molecule was identified from Apostichopus japonicus (designated as AjIRAK4) by RACE approach. The full-length cDNA of AjIRAK4 was of 2024 bp containing an open reading frame of 1311 bp encoding a 436-amino-acid (aa) residue polyprotein with the typical death domain (10-113aa) and the kinase domain (160-426aa). The mRNA transcripts of AjIRAK4 displayed constitutively expressed in all examined tissues with highest expression in the muscles (7.20-fold increase compared to the coelomocytes). The pathogen Vibrio splendidus challenge and LPS exposure could both significantly up-regulate the mRNA...
Source: Molecular Immunology - August 17, 2018 Category: Allergy & Immunology Source Type: research

Bradykinin-mediated Ca2+ signalling regulates cell growth and mobility in human cardiac c-Kit+ progenitor cells.
This study investigated whether bradykinin-mediated Ca2+ signalling participates in regulating cellular functions in cultured human cardiac c-Kit+ progenitor cells using laser scanning confocal microscopy and biochemical approaches. It was found that bradykinin increased cytosolic free Ca2+ (Cai2+) by triggering a transient Ca2+ release from ER IP3Rs followed by sustained Ca2+ influx through store-operated Ca2+ entry (SOCE) channel. Blockade of B2 receptor with HOE140 or IP3Rs with araguspongin B or silencing IP3R3 with siRNA abolished both Ca2+ release and Ca2+ influx. It is interesting to note that the bradykinin-induced...
Source: J Cell Mol Med - August 17, 2018 Category: Molecular Biology Authors: Li G, Che H, Wu WY, Jie LJ, Xiao GS, Wang Y, Li GR Tags: J Cell Mol Med Source Type: research

IFN- γ-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD.
IFN-γ-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD. Biochem J. 2018 Aug 17;: Authors: Unterer B, Wiesmann V, Gunasekaran M, Sticht H, Tenkerian C, Behrens J, Leone M, Engel FB, Britzen-Laurent N, Naschberger E, Wittenberg T, Stürzl M Abstract Interferon-gamma (IFN‑γ) is a pleiotropic cytokine that exerts important functions in inflammation, infectious diseases and cancer. The large GTPase human guanylate-binding protein 1 (GBP‑1) is among the most strongly IFN‑γ-induced cellular proteins. Previously it has been shown t...
Source: The Biochemical Journal - August 17, 2018 Category: Biochemistry Authors: Unterer B, Wiesmann V, Gunasekaran M, Sticht H, Tenkerian C, Behrens J, Leone M, Engel FB, Britzen-Laurent N, Naschberger E, Wittenberg T, Stürzl M Tags: Biochem J Source Type: research

Transient inhibition of sphingosine kinases confers protection to influenza A virus infected mice.
Abstract Influenza continues to pose a threat to public health by causing illness and mortality in humans. Discovering host factors that regulate influenza virus propagation is vital for the development of novel drugs. We have previously reported that sphingosine kinase (SphK) 1 promotes influenza A virus (IAV) replication in vitro. Here we demonstrate that the other isoform of SphK, SphK2 promotes the replication of influenza A virus (IAV) in cultured cells, and temporary inhibition of SphK1 or SphK2 enhances the host defense against influenza in mice. IAV infection led to an increased expression and phosphorylat...
Source: Antiviral Research - August 17, 2018 Category: Virology Authors: Xia C, Seo YJ, Studstill CJ, Vijayan M, Wolf JJ, Hahm B Tags: Antiviral Res Source Type: research

Betulinic acid alleviates myocardial hypoxia/reoxygenation injury via inducing Nrf2/HO-1 and inhibiting p38 and JNK pathways.
Abstract Myocardial ischemia reperfusion injury (I/RI) can lead to acute myocardial infarction (MI), which is a common cardiovascular disease (CVD) leading to morbidity and mortality worldwide. Betulinic acid exerts protective effect on myocardial I/RI in rats; however, the mechanism remains unknown. The aim of the current study was to evaluate the effect of betulinic acid on myocardial I/RI in vitro and further investigate the underlying mechanism. Our results showed that betulinic acid increased cell viability and decreased lactate dehydrogenase (LDH) release in H9c2 cells exposed to H/R. Betulinic acid was foun...
Source: European Journal of Pharmacology - August 17, 2018 Category: Drugs & Pharmacology Authors: Wang D, Chen T, Liu F Tags: Eur J Pharmacol Source Type: research

IRF5 is increased in labouring myometrium and regulates pro-labour mediators
Preterm birth continues to be the leading cause of neonatal mortality and morbidities that can extend into adult life. Few treatment options stem from our incomplete understanding of the mechanisms of human labour and delivery. Activation of the inflammatory response in gestational tissues by inflammation and/or infection leads to the production of pro-inflammatory and pro-labour mediators, thus preterm birth. Interferon regulatory factor 5 (IRF5) has recently emerged as an important pro-inflammatory transcription factor involved in acute and chronic inflammation. The aims of this study were to determine the expression of ...
Source: Reproduction - August 17, 2018 Category: Reproduction Medicine Authors: Lim, R., Barker, G., Lappas, M. Tags: Research Source Type: research

Periostin promotes liver fibrogenesis by activating lysyl oxidase in hepatic stellate cells Molecular Bases of Disease
Liver fibrosis arises from dysregulated wound healing due to persistent inflammatory hepatic injury. Periostin is a nonstructural extracellular matrix protein that promotes organ fibrosis in adults. Here, we sought to identify the molecular mechanisms in periostin-mediated hepatic fibrosis. Hepatic fibrosis in periostin−/− mice was attenuated as evidenced by significantly reduced collagen fibril density and liver stiffness compared with those in WT controls. A single dose of carbon tetrachloride caused similar acute liver injury in periostin−/− and WT littermates, and we did not detect significant differences in tr...
Source: Journal of Biological Chemistry - August 17, 2018 Category: Chemistry Authors: Pradeep Kumar, Tekla Smith, Reben Raeman, Daniel M. Chopyk, Hannah Brink, Yunshan Liu, Todd Sulchek, Frank A. Anania Tags: Cell Biology Source Type: research

Ethanol Extract of Lycopus lucidus Turcz. ex Benth Inhibits Metastasis by Downregulation of Runx-2 in Mouse Colon Cancer Cells.
This study aims to elucidate the effects of ethanol extracts of LT (ELT) relative to the role of Runt-related transcription factor- (Runx-) 2 in the invasive and metastatic potentials of mouse colon cancer to determine the underlying mechanisms involved. ELT was evaluated for the antimetastasis activity using CT-26 colon cancer using wound healing, transwell matrigel, and western blot analysis. We used Runx-2-specific siRNA to further determine the relationship between Runx-2 and matrix metalloprotease- (MMP-) 9 in the migration and invasion of CT-26 cells. Runx-2 was first demonstrated to be a transcription factor that pl...
Source: Evidence-based Complementary and Alternative Medicine - August 16, 2018 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

Beta-secretase/BACE1 Promotes APP Endocytosis and Processing in the Endosomes and on Cell Membrane
Publication date: Available online 16 August 2018Source: Neuroscience LettersAuthor(s): Mingguang Wang, Tian Jing, Xuan Wang, Dan YaoAbstractAmyloid-β proteins deposition and aggregation occur in extracellular space and form neuritic plaques in Alzheimer’s disease (AD) brain. Beta-site amyloid precursor protein cleaving enzyme 1 (BACE1)/ β-secretase and γ-secretase Presenilin 1 (PSEN1) conduct sequential cleavage of amyloid- β precursor protein (APP) and yield amyloid- β proteins. However the details of the interactions of APP with the enzymes and transportation of catalytic products are unclear. Here we reveal dist...
Source: Neuroscience Letters - August 16, 2018 Category: Neuroscience Source Type: research

ASAP pH-Sensitive Nanocarrier-Mediated Codelivery of Simvastatin and Noggin siRNA for Synergistic Enhancement of Osteogenesis
ACS Applied Materials& InterfacesDOI: 10.1021/acsami.8b10521
Source: ACS Applied Materials and Interfaces - August 16, 2018 Category: Materials Science Authors: Jinsheng Huang, Chaowen Lin, Jintao Fang, Xiaoxia Li, Jin Wang, Shaohui Deng, Sheng Zhang, Wanhan Su, Xiaoreng Feng, Bin Chen, Du Cheng, Xintao Shuai Source Type: research

miR ‑197 promotes the invasion and migration of colorectal cancer by targeting insulin‑like growth factor‑binding protein 3.
In conclusion, we revealed that miR‑197 modulates IGFBP3 and therefore plays a critical role in regulating CRC migration and invasion. PMID: 30106114 [PubMed - as supplied by publisher]
Source: Oncology Reports - August 16, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Inhibitor of apoptosis protein ‑like protein‑2: A novel growth accelerator for breast cancer cells.
Inhibitor of apoptosis protein‑like protein‑2: A novel growth accelerator for breast cancer cells. Oncol Rep. 2018 Aug 03;: Authors: Zhu L, Zhou W, Zhu X, Xiang S, Wang S, Peng Y, Lu B, Tang P, Chen Q, Wu M, Peng X, Chen Z, Sun Z, Yang K, Xiang M, Yu D Abstract Although the inhibitor of apoptosis protein‑like protein‑2 (ILP‑2) has been shown as a serological biomarker for breast cancer, its effect on breast cancer cell growth remains elusive. The present study aimed to determine the role of ILP‑2 in breast cancer cell growth. We used immunohistochemistry to analyze ILP‑2 expression in 59 ...
Source: Oncology Reports - August 16, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MicroRNA ‑34b promotes proliferation, migration and invasion of Ewing's sarcoma cells by downregulating Notch1.
In conclusion, EWS‑FLI1 may modulate miR‑34b expression directly or indirectly, and miR‑34b potentially has an oncogenic role in Ewing's sarcoma by downregulating Notch1. PMID: 30106161 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - August 16, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Correlation between DEC1/DEC2 and epithelial ‑mesenchymal transition in human prostate cancer PC‑3 cells.
Correlation between DEC1/DEC2 and epithelial‑mesenchymal transition in human prostate cancer PC‑3 cells. Mol Med Rep. 2018 Aug 09;: Authors: Liu Q, Wu Y, Seino H, Haga T, Yoshizawa T, Morohashi S, Kijima H Abstract Differentiated embryonic chondrocyte (DEC) genes have been reported to be involved in the regulation of mammalian circadian rhythms, differentiation, apoptosis, the response to hypoxia and epithelial‑mesenchymal transition (EMT). Activation of transforming growth factor (TGF)‑β signaling is known to promote EMT for the development of metastatic castration‑resistant prostate cancer...
Source: Molecular Medicine Reports - August 16, 2018 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

GSE98514 Expression profiling of four replicates of MCF-7 cells treated with 100nM TCDD
Contributors : Sean Piwarski ; James Denvir ; Donald A Primerano ; Jun Fan ; Travis B SalisburySeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that binds pollutants, therapeutic drugs and endogenous ligands. The AHR is expressed in all breast cancer subtypes and it can switch the aggressiveness of breast cancer cells from low to high depending on the ligand that it binds. Jagged 1 (JAG1) is a NOTCH receptor ligand that is overexpressed in basal-like breast cancer. JAG1 promotes breast cancer progression in ...
Source: GEO: Gene Expression Omnibus - August 15, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Tunability of Biodegradable Poly(amine-co-ester) Polymers for Customized Nucleic Acid Delivery and Other Biomedical Applications.
Abstract Gene therapy promises to treat diseases that arise from genetic abnormalities by correcting the underlying cause of the disease rather than treating the associated symptoms. Successful transfer of nucleic acids into cells requires efficient delivery vehicles that protect the cargo and can penetrate the appropriate cellular barriers before releasing their contents. Many viral vectors and synthetic polycationic vectors for nucleic acid delivery do not translate well from in vitro to in vivo applications due to their instability and toxicity. We synthesized and characterized a library of biocompatible low ch...
Source: Biomacromolecules - August 15, 2018 Category: Biochemistry Authors: Kauffman AC, Piotrowski-Daspit AS, Nakazawa KH, Jiang Y, Datye A, Saltzman WM Tags: Biomacromolecules Source Type: research

Lamp2 inhibits epithelial-mesenchymal transition by suppressing Snail expression in HCC.
This study found that Lamp2 expression was significantly lower in HCC tissues than in adjacent nontumor tissues (ANTs), and its expression level correlated with HCC metastasis. Low Lamp2 expression was significantly correlated with the AFP serum level (> 20 ng/Ml, P = 0.024), capsular formation (absent, P = 0.024), and microvascular invasion (present, P < 0.001), and low expression of Lamp2 indicated a poor prognosis in HCC. LowLamp2 expression was an independent and significant risk factor for recurrence-free survival (RFS; P < 0.001) and overall survival (OS; P < 0.001) in HCC. In this study, we demonstrated ...
Source: Oncotarget - August 14, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

GSE102653 Genome wide expression change by SMURF1 knocking down in MCF-7 cells
Contributor : J ZhuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe aim to the investigate the role of SMURF1 in breast cancer progression. MCF-7 cells were used as the model and SMURF1 was silenced by siRNA.
Source: GEO: Gene Expression Omnibus - August 14, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Preliminary screening and functional analysis of circular RNAs associated with hepatic stellate cell activation.
CONCLUSION: The circRNAs expression profile of liver tissue had changed in fibrosis mouse model, and some of these circRNAs may be associated with HSC activation. For instance, mmu_circ_34116 would inhibit HSC activation. PMID: 30118889 [PubMed - as supplied by publisher]
Source: Gene - August 14, 2018 Category: Genetics & Stem Cells Authors: Zhou Y, Lv X, Qu H, Zhao K, Fu L, Zhu L, Ye G, Guo J Tags: Gene Source Type: research

Fragile Histidine Triad (FHIT), a Novel Modifier Gene in Pulmonary Arterial Hypertension.
CONCLUSIONS: This study highlights the importance of the novel BMPR2 modifier FHIT in PH and the clinical value of the repurposed drug Enzastaurin as a potential novel therapeutic strategy to improve PAH. PMID: 30107138 [PubMed - as supplied by publisher]
Source: Am J Respir Crit Car... - August 14, 2018 Category: Intensive Care Authors: Dannewitz Prosseda S, Tian X, Kuramoto K, Boehm M, Sudheendra D, Miyagawa K, Zhang F, Solow-Cordero D, Saldivar JC, Austin ED, Loyd JE, Wheeler L, Andruska A, Donato M, Wang L, Huebner K, Metzger RJ, Khatri P, Spiekerkoetter E Tags: Am J Respir Crit Care Med Source Type: research

The differentiation of human MSCs derived from adipose and amniotic tissues into insulin-producing cells, induced by PEI@Fe3O4 nanoparticles-mediated NRSF and SHH silencing.
Abstract Type 1 diabetes involves the immunologically mediated destruction of insulin‑producing cells (IPCs) in the pancreatic islet. Mesenchymal stem cells (MSCs) have the ability to differentiate into IPCs and have become the most promising means for diabetes therapy. The present study demonstrated that human adipose‑derived stem cells (hADSCs) and human amniotic MSCs (hAMSCs) are able to differentiate into functional IPCs by knocking down neuronal restrictive silencing factor (NRSF) and Sonic hedgehog (SHH). In the current study, PEI@Fe3O4 nanoparticles (NPs) were used to deliver NRSF small interfering (si)...
Source: International Journal of Molecular Medicine - August 14, 2018 Category: Molecular Biology Authors: Wang R, Zhang D, Zhang T, Zhao F, Lang H, Lin X, Pang X Tags: Int J Mol Med Source Type: research

HDL activates expression of genes stimulating cholesterol efflux in human monocyte-derived macrophages.
In this study, we looked for additional proteins contributing to HDL-dependent cholesterol efflux. Using RNAseq, we analyzed mRNAs induced by HDL in human monocyte-derived macrophages and identified three genes, fatty acid desaturase 1 (FADS1), insulin induced gene 1 (INSIG1), and the low-density lipoprotein receptor (LDLR), expression of which was significantly upregulated by HDL. We individually knocked down these genes in THP-1 cells using gene silencing by siRNA, and measured cellular cholesterol efflux to HDL. Knock down of FADS1 did not significantly change cholesterol efflux (p = 0.70), but knockdown of INSIG1 a...
Source: Experimental and Molecular Pathology - August 14, 2018 Category: Pathology Authors: Orekhov AN, Pushkarsky T, Oishi Y, Nikiforov NG, Zhelankin AV, Dubrovsky L, Makeev VJ, Foxx K, Jin X, Kruth HS, Sobenin IA, Sukhorukov VN, Zakiev ER, Kontush A, Le Goff W, Bukrinsky M Tags: Exp Mol Pathol Source Type: research

JAGN1 is required for fungal killing in neutrophil extracellular traps: Implications for severe congenital neutropenia.
Abstract Mutations in the gene JAGN1 were recently discovered in patients with severe congenital neutropenia (SCN). Neutrophils release neutrophil extracellular traps (NETs) consisting of decondensed chromatin decorated with various granular proteins such as neutrophil elastase and myeloperoxidase (MPO) to combat microbial infections. However, whether JAGN1 is required for the formation or function of NETs is not known. Here, we analyzed primary neutrophils from a patient with homozygous JAGN1 mutations with respect to phorbol myristate acetate (PMA)-induced NET formation. NET release was observed, but there appea...
Source: Journal of Leukocyte Biology - August 14, 2018 Category: Hematology Authors: Khandagale A, Lazzaretto B, Carlsson G, Sundin M, Shafeeq S, Römling U, Fadeel B Tags: J Leukoc Biol Source Type: research

Functional coupling of GABAA/B receptors and the channel TRPV4 mediates rapid progesterone signaling in the oviduct.
We report a rapid response to P4 and agonists of γ-aminobutyric acid receptors A and B (GABAA/B) in the mouse oviduct that was characterized by oscillatory Ca2+ signals and increased ciliary beat frequency (CBF). Pharmacological manipulation, genetic ablation, and siRNA-mediated knockdown in oviductal cells, as well as overexpression experiments in HEK 293T cells, confirmed the participation of the cationic channel TRPV4, different subunits of GABAA (α1 to α3, β2, and β3), and GABAB1 in P4-induced responses. TRPV4-mediated Ca2+ entry in close proximity to the inositol trisphosphate receptor was required to initiate an...
Source: Science Signaling - August 14, 2018 Category: Biomedical Science Authors: Jung C, Fernández-Dueñas V, Plata C, Garcia-Elias A, Ciruela F, Fernández-Fernández JM, Valverde MA Tags: Sci Signal Source Type: research

FOXO3a-dependent up-regulation of Mxi1-0 promotes hypoxia-induced apoptosis in endothelial cells.
In this study, we found that hypoxia increased Mxi1-0 expression, and the Mxi1-0 siRNA could inhibit caspase-8 activation and apoptosis in HUVECs induced by hypoxia. In addition, hypoxia induced FOXO3 activation, while Mxi1-0 expression and apoptosis were inhibited by transfection with FOXO3 siRNA. Using ChIP assay, we confirmed that FOXO3a binds to the Mxi1-0 promoter region. Furthermore, hypoxia treatment leads to remarkable production of reactive oxygen species (ROS), while ROS scavenger N-acetyl-L-cysteine inhibits hypoxia-induced ROS production, apoptosis and FOXO3a-mediated Mxi1-0 up-regulation. Finally, we found tha...
Source: Cellular Signalling - August 14, 2018 Category: Cytology Authors: Hu Z, Wang F, Wu Z, Gu H, Dong N, Jiang X, Xu J, Wu Z, Wechsler DS, Zheng D Tags: Cell Signal Source Type: research

Functional coupling of GABAA/B receptors and the channel TRPV4 mediates rapid progesterone signaling in the oviduct
We report a rapid response to P4 and agonists of -aminobutyric acid receptors A and B (GABAA/B) in the mouse oviduct that was characterized by oscillatory Ca2+ signals and increased ciliary beat frequency (CBF). Pharmacological manipulation, genetic ablation, and siRNA-mediated knockdown in oviductal cells, as well as overexpression experiments in HEK 293T cells, confirmed the participation of the cationic channel TRPV4, different subunits of GABAA (α1 to α3, β2, and β3), and GABAB1 in P4-induced responses. TRPV4-mediated Ca2+ entry in close proximity to the inositol trisphosphate receptor was require...
Source: Signal Transduction Knowledge Environment - August 14, 2018 Category: Science Authors: Jung, C., Fernandez-Duenas, V., Plata, C., Garcia-Elias, A., Ciruela, F., Fernandez-Fernandez, J. M., Valverde, M. A. Tags: STKE Research Articles Source Type: news

Silencing of PHLPP1 promotes neuronal apoptosis and inhibits functional recovery after spinal cord injury in mice
In this study, we investigated the effects of the progression of SCI and potential regulation of apoptosis after the Pleckstrin homology (PH) domain and leucine rich repeat protein phosphatase 1 (PHLPP1) gene was silenced.Main methodsSpinal cord injection, and neuronal transfection with a recombinant adenovirus vector encoding small interfering RNA (siRNA) against PHLPP1 (AdsiPHLPP1) successfully silenced PHLPP1. These created in vivo and in vitro PHLPP1-silenced models, respectively, resulting in stable expression of the transgene in neurons.Key findingsThe results showed that silencing of PHLPP1 evidently reduced levels ...
Source: Life Sciences - August 13, 2018 Category: Biology Source Type: research

15-lipoxygenase-2/15(S)-hydroxyeicosatetraenoic acid regulates cell proliferation and metastasis via the STAT3 pathway in lung adenocarcinoma
This study may provide a greater understanding of LAC metastasis and shed new light on the mechanisms by which the 15(S)-HETE/STAT3 pathway is related to this disease.
Source: Prostaglandins and Other Lipid Mediators - August 13, 2018 Category: Lipidology Source Type: research

Rab27a Regulates Human Perivascular Adipose Progenitor Cell Differentiation
ConclusionsPVAT-APC from CABG donors express common adipocyte progenitor markers and differentiate into UCP1-containing adipocytes. Rab27a has an endogenous role in promoting the maturation of adipocytes from human PVAT-derived APC.
Source: Cardiovascular Drugs and Therapy - August 13, 2018 Category: Cardiology Source Type: research

Characterizing the interaction between insulin-like growth factor 2 mRNA-binding protein 1 (IMP1) and KRAS expression.
This study shows that the regulation of KRAS expression by IMP1 is complex and may involve both the IMP1 protein as well as its mRNA transcript. PMID: 30104206 [PubMed - as supplied by publisher]
Source: The Biochemical Journal - August 13, 2018 Category: Biochemistry Authors: Mackedenski S, Wang C, Li WM, Lee CH Tags: Biochem J Source Type: research

Fos-like antigen 2 (FOSL2) promotes metastasis in colon cancer.
Abstract Among different cancers, incidence and mortality of colorectal cancer (CRC) is one of the highest. KRAS mutation is one of the underlying features in the pathogenesis of CRC with CRC tumors harboring mutant KRAS exhibiting a more aggressive behavior compared to CRC tumors with wild type KRAS. We had earlier shown that the microRNA-143 (miR-143) replenishment not only chemosensitizes CRC cell line with mutant KRAS instead of wild-type KRAS gene, to paclitaxel-mediated cytotoxicity, but also inhibits cell migration and invasion ability. Hence, the study aimed to determine how miR-143 replenishment is inhibi...
Source: Experimental Cell Research - August 13, 2018 Category: Cytology Authors: Fei BY, Wang XY, Fang XD Tags: Exp Cell Res Source Type: research

Rab27a Regulates Human Perivascular Adipose Progenitor Cell Differentiation
ConclusionsPVAT-APC from CABG donors express common adipocyte progenitor markers and differentiate into UCP1-containing adipocytes. Rab27a has an endogenous role in promoting the maturation of adipocytes from human PVAT-derived APC.
Source: Cardiovascular Drugs and Therapy - August 13, 2018 Category: Cardiology Source Type: research

Salidroside alleviates Ischemic Brain Injury in Mice with Ischemic Stroke through regulating BDNK mediated PI3K/Akt Pathway.
In this study, We preformed MCAO model in C57BL/6J wild-type (BDNK+/+) and BDNK knockout (BDNF-/-) mice respectively, and investigated the neuroprotective effect of Salidroside (Sal) and its underlying mechanisms. The results showed that Sal reversed brain infarct size, reduced cerebral edema, decreased the neurological deficit score and diminished TUNEL positive cells significantly. However, BDNK deficiency inhibited the neuroprotective effect of Sal. In addition, Sal increased cell viability, ameliorated neuron cell injury by decreasing LDH activity, and inhibited cell apoptotic rate. Sal suppressed apoptotic signaling v...
Source: Biochemical Pharmacology - August 13, 2018 Category: Drugs & Pharmacology Authors: Zhang X, Du Q, Yang Y, Wang J, Liu Y, Zhao Z, Zhu Y, Liu C Tags: Biochem Pharmacol Source Type: research

SRPK1 gene silencing promotes vascular smooth muscle cell proliferation and vascular remodeling via inhibition of the PI3K/Akt signaling pathway in a rat model of intracranial aneurysms.
CONCLUSION: Our results show that siRNA-mediated silencing of SRPK1 gene inhibits VSMC apoptosis, and increases VSMCs proliferation and vascular remodeling in IA via the PI3 K/Akt signaling pathway. Our findings provide a novel intervention target for the molecular treatment of IA. PMID: 30101479 [PubMed - as supplied by publisher]
Source: CNS Neuroscience and Therapeutics - August 12, 2018 Category: Neuroscience Authors: Li XG, Wang YB Tags: CNS Neurosci Ther Source Type: research

SIRT1-dependent epigenetic regulation of H3 and H4 histone acetylation in human breast cancer.
In this study, we elucidated SIRT1 epigenetic role and analyzed the link between the latter and histones H3 and H4 epigenetic marks in all 5 molecular subtypes of breast cancer. Using a cohort of 135 human breast tumors and their matched normal tissues, as well as 5 human-derived cell lines, we identified H3k4ac as a new prime target of SIRT1 in breast cancer. We also uncovered an inverse correlation between SIRT1 and the 3 epigenetic marks H3k4ac, H3k9ac and H4k16ac expression patterns. We showed that SIRT1 modulates the acetylation patterns of histones H3 and H4 in breast cancer. Moreover, SIRT1 regulates its H3 acetylat...
Source: Oncotarget - August 11, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

The nuclear-cytoplasmic trafficking of a chromatin-modifying and remodelling protein (KMT2C), in osteosarcoma.
Authors: Chiappetta C, Puggioni C, Carletti R, Petrozza V, Della Rocca C, Di Crisfofano C Abstract Osteosarcoma is the most common paediatric primary non-hematopoietic bone tumor; the survival is related to the response to chemotherapy and development of metastases. KMT2C is a chromatin-modifying and remodelling protein and its expression has never been studied in osteosarcoma. The aim of this study was to understand the role of KMT2C in the osteosarcoma carcinogenesis and metastatic progression to identify a new molecular target and to provide new therapeutic approach. We performed the immunohistochemical and gene...
Source: Oncotarget - August 11, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Osmotic and hypoxic induction of the complement factor C9 in cultured human retinal pigment epithelial cells: Regulation of VEGF and NLRP3 expression.
Conclusions: The expression of the C9 gene in cultured RPE cells is highly induced by extracellular hyperosmolarity, hypoxia, and oxidative stress. The data may support the assumption that C9 gene expression may stimulate the expression of inflammatory (NLRP3) and angiogenic growth factors (VEGF) in RPE cells. Extracellular C9 protein may attenuate this effect, in part via negative regulation of the C9 mRNA level. PMID: 30090015 [PubMed - in process]
Source: Molecular Vision - August 11, 2018 Category: Molecular Biology Tags: Mol Vis Source Type: research

Resveratrol alleviates sepsis-induced acute lung injury by suppressing inflammation and apoptosis of alveolar macrophage cells.
Authors: Yang L, Zhang Z, Zhuo Y, Cui L, Li C, Li D, Zhang S, Cui N, Wang X, Gao H Abstract Sepsis is a major cause of death in intensive care units. The purpose of this study was to investigate the effect of resveratrol (RSV) on sepsis-induced acute lung injury (ALI). The underlying molecular mechanisms were deciphered by both in vitro and in vivo experiments. Polymicrobial sepsis was induced in C57BL/6 mice by cecal ligation and puncture (CLP). RSV pretreatment significantly attenuated CLP-induced acute lung injury, which was associated with enhanced expression of VEGF-B. The protective properties of RSV were ass...
Source: American Journal of Translational Research - August 11, 2018 Category: Research Tags: Am J Transl Res Source Type: research

MicroRNA-449a suppresses hepatocellular carcinoma cell growth via G1 phase arrest and the HGF/MET c-Met pathway
ConclusionThe tumor suppressor miR-449a suppresses HCC tumorigenesis by repressing the c-Met/ERK pathway.
Source: Hepatobiliary and Pancreatic Diseases International - August 11, 2018 Category: Gastroenterology Source Type: research

Effects of Oncostatin M on Invasion of Primary Trophoblasts under Normoxia and Hypoxia Conditions.
CONCLUSION: OSM might be involved in the invasiveness of extravillous trophoblasts under hypoxia conditions via increasing MMP-2 and MMP-9 enzymatic activities through STAT3 signaling. Increased MMP-9 activity by OSM seems to be more important in primary trophoblasts. PMID: 30091322 [PubMed - in process]
Source: Yonsei Medical Journal - August 11, 2018 Category: Universities & Medical Training Authors: Wie JH, Ko HS, Choi SK, Park IY, Kim A, Kim HS, Shin JC Tags: Yonsei Med J Source Type: research

Inhibition of Epidermal Growth Factor Receptor (EGFR) Reduces Lipopolysaccharide (LPS)-Induced Activation and Inflammatory Cytokines in Hepatic Stellate Cells In Vitro.
CONCLUSIONS Activation of HSCs by LPS in vitro, including the expression of inflammatory cytokines and mediators of fibrogenesis, were shown to be dependent on the expression of EGFR. PMID: 30091424 [PubMed - in process]
Source: Medical Science Monitor - August 10, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Contribution of human UDP-glucuronosyltransferases to the antioxidant effects of propolis, artichoke and silymarin
Conclusion: In conclusion, this study provides a possible molecular mechanism for protective antioxidant effects associated with the herbal drugs propolis, artichoke and silymarin. The herbal drug-mediated transcriptional upregulation of human detoxifying UGT1A enzymes via activation of AhR and Nrf2 leads to reduced hydrogen peroxide and oxidative stress. Because of UGT1A activation, the intake of these drugs could affect the therapeutic efficacy of other drugs when these also undergo metabolism by glucuronidation.Graphical abstract
Source: Phytomedicine - August 10, 2018 Category: Drugs & Pharmacology Source Type: research

FDA approves first-of-its kind targeted RNA-based therapy to treat a rare disease
FDA approves new drug for treatment of polyneuropathy caused by hereditary transthyretin-mediated amyloidosis (hATTR). This is the first FDA-approved treatment for this rare, debilitating and often fatal genetic disease and the first FDA approval of a new class of drugs called small interfering ribonucleic acid (siRNA) treatment.
Source: Food and Drug Administration - August 10, 2018 Category: American Health Source Type: news

Mir-758-5p Suppresses Glioblastoma Proliferation, Migration and Invasion by Targeting ZBTB20
Conclusion: miR-758-5p suppressed GBM progression by targeting ZBTB20. The miR-758-5p/ZBTB20 axis might be a promising therapeutic target for GBM treatment.Cell Physiol Biochem 2018;48:2074 –2083
Source: Cellular Physiology and Biochemistry - August 10, 2018 Category: Cytology Source Type: research

Early missed abortion is associated with villous angiogenesis via the HIF-1 α/VEGF signaling pathway
ConclusionsOur results reveal a novel role for HIF-1 α/VEGF in regulating villous angiogenesis in early pregnancy and suggest that it may be a novel biomarker for missed abortion.
Source: Archives of Gynecology and Obstetrics - August 10, 2018 Category: OBGYN Source Type: research

GSE102498 JCAD/KIAA1462, a coronary artery disease-associated gene product, regulates endothelial function
Contributors : Suowen Xu ; Zheng G Jin ; John M Ashton ; Jason R MyersSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRecent genome-wide association studies (GWAS) have identified gene variants associated with coronary artery disease including ADAMTS7, PHACTR1, KIAA1462/JCAD (Junctional Protein Associated with Coronary Artery Disease) and many others. JCAD has been identified as a novel component of endothelial cell-cell junctions (Akashi et al., 2011, BBRC) and regulates angiogenesis (Hara et al, ATVB, 2017). In our study, we observed that JCAD is a 148-KDa protein identified by mass...
Source: GEO: Gene Expression Omnibus - August 10, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Novel endocrine therapeutic strategy in endometrial carcinoma targeting estrogen-related receptor & alpha; by XCT790 and siRNA
Source: Cancer Management and Research - August 10, 2018 Category: Cancer & Oncology Tags: Cancer Management and Research Source Type: research

Icariin reduces human colon carcinoma cell growth and metastasis by enhancing p53 activities.
Authors: Tian M, Yang S, Yan X Abstract Icariin has been reported to possess high anticancer activity. Colon carcinoma is one of the leading causes of cancer-related mortality worldwide. Here, the anticancer activity of icariin against HCT116 colon carcinoma cells and the possible underlying mechanism were studied. The trypan blue staining assay, wound healing assay, clonogenic assay, CCK-8 assay, and Annexin V-FITC/PI double staining method were carried out to determine the changes of HCT116 cell growth and migration. mRNA and protein expressions were determined by quantitative real-time PCR and western blot, resp...
Source: Brazilian Journal of Medical and Biological Research - August 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research