Query: siRNA

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Total 13806 results found since Jan 2013.

Ruthenium dendrimers as carriers for anticancer siRNA.
This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into...
Source: Journal of Inorganic Biochemistry - January 12, 2018 Category: Biochemistry Authors: Michlewska S, Ionov M, Maroto-Díaz M, Szwed A, Ihnatsyeu-Kachan A, Loznikova S, Shcharbin D, Maly M, Ramirez RG, de la Mata FJ, Bryszewska M Tags: J Inorg Biochem Source Type: research

Ultrasound-mediated nanobubble destruction (UMND) facilitates the delivery of A10-3.2 aptamer targeted and siRNA-loaded cationic nanobubbles for therapy of prostate cancer.
In this study, we synthesized a promising anti-tumor targeted FoxM1 siRNA-loaded cationic nanobubbles (CNBs) conjugated with an A10-3.2 aptamer (siFoxM1-Apt-CNBs), which demonstrate high specificity when binding to prostate-specific membrane antigen (PSMA) positive LNCaP cells. Uniform nanoscaled siFoxM1-Apt-CNBs were developed using a thin-film hydration sonication, carbodiimide chemistry approaches, and electrostatic adsorption methods. Fluorescence imaging as well as flow cytometry evidenced the fact that the siFoxM1-Apt-CNBs were productively developed and that they specifically bound to PSMA-positive LNCaP cells. siFo...
Source: Drug Delivery - January 11, 2018 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research

VEGF siRNA delivery by a cancer-specific cell-penetrating peptide.
In this study, we investigated the efficacy of the cancer-specific CPP carrier BR2 to specifically transport siRNA to cancer-target cells. Our results showed that BR2 formed a complex with anti-vascular endothelial growth factor siRNA (siVEGF) that exhibited the appropriate size and surface charge for in vivo treatment. Additionally, the BR2-VEGF siRNA complex exhibited significant serum stability and high levels of gene-silencing effects in vitro. Moreover, the transfection efficiency of the complex into a cancer cell line was higher than that observed in non-cancer cell lines, resulting in downregulated intracellular VEG...
Source: Journal of Microbiology and Biotechnology - January 11, 2018 Category: Biotechnology Authors: Lee YW, Hwang YE, Lee JY, Sohn JH, Sung BH, Kim SC Tags: J Microbiol Biotechnol Source Type: research

Effects of siRNA-dependent knock-down of cardiolipin synthase and tafazzin on mitochondria and proliferation of glioma cells
This study aimed to separate the effects of CL content and CL composition from cellular free fatty acid distribution on bioenergetics and proliferation in C6 glioma cells. To this end, cardiolipin synthase and the CL remodelling enzyme, tafazzin, were knocked-down by siRNA in C6 cells. After 72 h of cultivation, we analysed CL composition by means of LC/MS/MS, distribution of cellular fatty acids by means of gas chromatography, and determined oxygen consumption and proliferation. Knock-down of cardiolipin synthase affected the cellular CL content in the presence of linoleic acid (LA) in the culture medium. Knock-down of ta...
Source: Biochimica et Biophysica Acta (BBA) Molecular and Cell Biology of Lipids - January 10, 2018 Category: Lipidology Source Type: research

Effects of siRNA-dependent knock-down of cardiolipin synthase and tafazzin on mitochondria and proliferation of glioma cells.
This study aimed to separate the effects of CL content and CL composition from cellular free fatty acid distribution on bioenergetics and proliferation in C6 glioma cells. To this end, cardiolipin synthase and the CL remodelling enzyme, tafazzin, were knocked-down by siRNA in C6 cells. After 72 h of cultivation, we analysed CL composition by means of LC/MS/MS, distribution of cellular fatty acids by means of gas chromatography, and determined oxygen consumption and proliferation. Knock-down of cardiolipin synthase affected the cellular CL content in the presence of linoleic acid (LA) in the culture medium. Knock-down of ta...
Source: Biochimica et Biophysica Acta - January 8, 2018 Category: Biochemistry Authors: Ohlig T, Le DV, Gardemann A, Wolke C, Gürtler S, Peter D, Schild L, Lendeckel U Tags: Biochim Biophys Acta Source Type: research

siRNA-mediated c-Rel knockdown ameliorates collagen-induced arthritis in mice.
Abstract Previous studies have shown that inflammatory mediators involved in the development of rheumatoid arthritis (RA) are regulated by the Rel/nuclear factor-κB (Rel/NF-κB) transcription factor family. c-Rel, a member of the Rel/NF-κB family that is preferentially expressed by immune cells, is a risk factor for several inflammatory diseases including RA. In the current study, we investigated whether targeting c-Rel can be used to treat collagen-induced arthritis, an animal model for RA. c-Rel specific siRNA (siRel) delivered by nanoparticles was used to knockdown the expression of c-Rel. Our results showed ...
Source: International Immunopharmacology - January 8, 2018 Category: Allergy & Immunology Authors: Fan T, Zhong F, Liu R, Chen YH, Wang T, Ruan Q Tags: Int Immunopharmacol Source Type: research

GSE100813 Transcriptome analysis of FKBP25 siRNA-knockdown in cultured HEK 293 cells
Contributors : David Dilworth ; Christopher J NelsonSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPeptidyl-prolyl isomerases (PPIs) are a superfamily of ubiquitous enzymes that catalyze the cis-trans interconversion of Xaa-Pro peptide bonds. The functions of most PPIs remain uncharacterized. FKBP25, a mammalian PPI, localizes to the nucleus, binds nucleic acids, and associates with chromatin modifying enzymes. However, the role of this protein in transcriptional regulation remains unclear. To help address this question we present RNA-seq gene expression profiling in HEK293 cells tra...
Source: GEO: Gene Expression Omnibus - January 3, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

4 co-delivery of tnf- α sirna and il-22 via a nanoparticle-in-hydrogel system exerts synergistic therapeutic effects against ulcerative colitis
Combination therapy is an emerging approach for treating ulcerative colitis (UC). During the development of UC, TNF- α acts as the major pro-inflammatory cytokine while interleukin-22 (IL-22) plays an important role in preventing mucosal damage. In the present study, we loaded TNF-α siRNA (siTNF) into galactosylated polymeric nanoparticles (NPs). The resultant Gal-siTNF-NPs had a desirable diameter (~261 nm), narrow size distribution and a slightly negative zeta potential (~‒12 mV). These NPs successful mediated the targeted delivery of siTNF to macrophages and efficiently inhibited the expression of TNF-α.
Source: Gastroenterology - January 1, 2018 Category: Gastroenterology Authors: Bo Xiao, Emilie Viennois, Tim L. Denning, Didier Merlin Tags: Clinical and Research Challenges Source Type: research

Exosomes Serve as Nano ‐particles to Suppress Tumor Growth and Angiogenesis in Gastric Cancer by Delivering HGF siRNA
This article is protected by copyright. All rights reserved.
Source: Cancer Science - December 29, 2017 Category: Cancer & Oncology Authors: Haiyang Zhang, Yi Wang, Ming Bai, Junyi Wang, Kegan Zhu, Rui Liu, Shaohua Ge, JiaLu Li, Tao Ning, Ting Deng, Qian Fan, Hongli Li, Wu Sun, Guoguang Ying, Yi Ba Tags: Original Article Source Type: research

Improved method for synthesis of low molecular weight protamine –siRNA conjugate
Publication date: Available online 19 December 2017 Source:Acta Pharmaceutica Sinica B Author(s): Zhili Yu, Junxiao Ye, Xing Pei, Lu Sun, Ergang Liu, Jianxin Wang, Yongzhuo Huang, Seung Jin Lee, Huining He RNAi technology has aroused wide public interest due to its high efficiency and specificity to treat multiple types of diseases. However, the effective delivery of siRNA remains a challenge due to its large molecular weight and strong anionic charge. Considering their remarkable functions in vivo and features that are often desired in drug delivery carriers, biomimetic systems for siRNA delivery become an effective and ...
Source: Acta Pharmaceutica Sinica B - December 20, 2017 Category: Cancer & Oncology Source Type: research

Effects of LncRNA BC168687 siRNA on Diabetic Neuropathic Pain Mediated by P2X7 Receptor on SGCs in DRG of Rats.
In conclusion, lncRNA BC168687 may participate in the pathogenesis of DNP mediated by P2X7 receptor, which will provide a novel way for the study of the pathogenesis of diabetes mellitus complicated with neuropathic pain and its prevention and treatment. PMID: 29204447 [PubMed - in process]
Source: Biomed Res - December 7, 2017 Category: Research Authors: Liu C, Tao J, Wu H, Yang Y, Chen Q, Deng Z, Liu J, Xu C Tags: Biomed Res Int Source Type: research

siRNA delivery with chitosan: influence of chitosan molecular weight, degree of deacetylation and amine to phosphate ratio on in vitro silencing efficiency, hemocompatibility, biodistribution and in vivo efficacy.
Abstract Chitosan (CS) shows in vitro and in vivo efficacy for siRNA delivery, but with contradictory findings for incompletely characterized systems. To understand which parameters produce effective delivery, a library of precisely characterized chitosans was produced at different DDAs and Mn. Encapsulation and transfection efficiencies were characterized in vitro. Formulations were selected to examine the influence of Mn and N:P ratio on nanoparticle uptake, metabolic activity, genotoxicity, and in vitro transfection. Hemocompatibility and in vivo biodistribution were then investigated for different Mn, N:P rati...
Source: Biomacromolecules - December 6, 2017 Category: Biochemistry Authors: Alameh MG, Lavertu M, Tran-Khanh N, Chang CY, Lesage F, Bail M, Darras V, Chevrier A, Buschmann MD Tags: Biomacromolecules Source Type: research

07 The approach to generate oncolytic adenovirus by sirna mediated e1b silencing
ConclusionIn addition to knockout of the E1B coding cassette, siRNA mediated E1B also serves as an approach to prepare oncolytic adenovirus.AcknowledgementsSupported by grants from UICC (ICRETT Fellowship), Guangdong Commission of Health and Family Plan (Grant No. 2014A276).
Source: Journal of Investigative Medicine - December 5, 2017 Category: Research Authors: Zhang, X., Matskova, L., Zheng, B., Ernberg, I., He, Z., Huang, Z. Tags: Abstracts Source Type: research

Inhibition of HBV replication by delivering the dual-gene expression vector pHsa-miR16-siRNA in HepG2.2.15 cells
This study aimed to construct the dual-gene expression vector pHsa-miR16-siRNA which can express human miR-16 and HBV X siRNA, and examine its regulatory effect on HBV gene expression in the HepG2.2.15 cell line. The expression vectors siR-1583 and pHsa-miR16-siRNA were designed and constructed. HepG2.2.15 cells were transfected with the empty vector, siR-1583, pmiR-16 and pHsa-miR16-siRNA, respectively. ELISA was performed to measure the expression of HBsAg and HBeAg in the culture supernatant 48 and72 h post transfection. Fluorescence quantitative PCR was used to measure the HBV mRNA degradation efficiency and HBV DNA co...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2017 Category: Research Source Type: research

Well-defined cationic N-3-(dimethylamino)propylmethacrylamide hydrochloride based (co)polymers for siRNA delivery.
Abstract Cationic glycopolymers have shown to be excellent candidates for the fabrication of gene delivery devices due to their ability to electrostatically interact with negatively charged nucleic acids and the carbohydrate residues ensure enhanced stability and low toxicity of the polyplexes. The ability to engineer the polymers for optimized compositions, molecular weights and architectures is critical in the design of effective gene delivery vehicles. Therefore, in this study, the aqueous reversible addition-fragmentation chain transfer polymerization (RAFT) was used to synthesize well-defined cationic glycopo...
Source: Biomacromolecules - December 1, 2017 Category: Biochemistry Authors: Singhsa P, Diaz-Dussan D, Manuspiya H, Narain R Tags: Biomacromolecules Source Type: research

RNAi mechanisms in Huntington ’s disease therapy: siRNA versus shRNA
AbstractHuntington ’s Disease (HD) is a genetically dominant trinucleotide repeat disorder resulting from CAG repeats within the Huntingtin (HTT) gene exceeding a normal range (>  36 CAGs). Symptoms of the disease manifest in middle age and include chorea, dystonia, and cognitive decline. Typical latency from diagnosis to death is 20 years. There are currently no disease-modifying therapies available to HD patients. RNAi is a potentially curative therapy for HD. A popular line of research employs siRNA or antisense oligonucleotides (ASO) to knock down mutant Huntingtin mRNA (mHTT). Unfortunately, this modality requ...
Source: Translational Neurodegeneration - November 27, 2017 Category: Neurology Source Type: research

Cyclodextrin-siRNA conjugates as versatile gene silencing agents.
Abstract Functional siRNAs (luciferase and PLK1) have been conjugated to β-cyclodextrin and the ability of the conjugates to retain gene knockdown activity has been assessed by delivery to cancer cell lines using various formulations. Initially two formulations used complexation with polycations, namely Lipofectamine 2000 and an amphiphilic polycationic cyclodextrin. Gene knockdown results for human glioblastoma cells (U87) and prostate cancer cells (PC3, DU145) showed that conjugation to the cyclodextrin did not reduce gene silencing by the RNA. A third mode of delivery involved formation of targeted nanoparticl...
Source: European Journal of Pharmaceutical Sciences - November 27, 2017 Category: Drugs & Pharmacology Authors: Malhotra M, Gooding M, Evans JC, O'Driscoll D, Darcy R, O'Driscoll C Tags: Eur J Pharm Sci Source Type: research

07 The approach to generate oncolytic adenovirus by sirna mediated e1b silencing
Conclusion In addition to knockout of the E1B coding cassette, siRNA mediated E1B also serves as an approach to prepare oncolytic adenovirus. Acknowledgements Supported by grants from UICC (ICRETT Fellowship), Guangdong Commission of Health and Family Plan (Grant No. 2014A276).
Source: Journal of Investigative Medicine - November 24, 2017 Category: Research Authors: Zhang, X., Matskova, L., Zheng, B., Ernberg, I., He, Z., Huang, Z. Tags: Abstracts Source Type: research

Inhibition of IL-8-mediated endothelial adhesion, VSMCs proliferation and migration by siRNA-TMEM98 suggests TMEM98's emerging role in atherosclerosis.
Authors: Lv G, Zhu H, Li C, Wang J, Zhao D, Li S, Ma L, Sun G, Li F, Zhao Y, Gao Y Abstract Transmembrane protein 98 (TMEM98), known as a novel gene related to lung cancer, hepatocellular carcinoma, differentiation of T helper 1 cells and normal eye development, has no defined role reported in terms of atherosclerosis (AS). To investigate the potential involvement of TMEM98 during AS processes, its obvious secretion and expression has been initially characterized in hyperlipidemia patients' serum and AS mice's serum respectively. We then explored the possible role of TMEM98 in the pathogenesis of AS in vitro. IL-8,...
Source: Oncotarget - November 22, 2017 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Retracted: Silencing of the COPS3 Gene by siRNA Reduces Proliferation of Lung Cancer Cells Most Likely via Induction of Cell Cycle Arrest and Apoptosis
Authors: Abstract Retraction: Retracted: siRNA mediated silencing of NIN1/RPN12 binding protein 1 homolog inhibits proliferation and growth of breast cancer cells Asian Pacific Journal of Cancer Prevention has retracted the article titled “siRNA mediated silencing of NIN1/RPN12 binding protein 1 homolog inhibits proliferation and growth of breast cancer cells”(1) for reason of similarity with a series of articles identified by Byrne and Labbé (2). 1. Huang WY1, Chen DH, Ning L, Wang LW. siRNA mediated silencing of NIN1/RPN12 binding protein 1 homolog inhibits proliferation and growth of breast cancer cells. A...
Source: Asian Pacific Journal of Cancer Prevention - November 19, 2017 Category: Cancer & Oncology Tags: Asian Pac J Cancer Prev Source Type: research

SRPK1 ‑siRNA suppresses K562 cell growth and induces apoptosis via the PARP‑caspase3 pathway.
In conclusion, the knockdown of the SRPK1 gene by RNAi inhibited the proliferation of K562 cells and induced their apoptosis. Apoptosis was induced by the activation of the PARP‑caspase3 pathway. PMID: 29138847 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - November 16, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Controlling miRNA-like Off-target Effects of an siRNA with Nucleobase Modifications
Org. Biomol. Chem., 2017, Accepted Manuscript DOI: 10.1039/C7OB02654D, PaperScott Ryan Suter, Alexi A Ball-Jones, Madeline M. Mumbleau, Rachel Valenzuela, Jose M. Ibarra-Soza, Hassan Owens, Andrew Fisher, Peter A Beal SiRNAs can cause unintended gene silencing due to miRNA-like effects because of the similarity in function of an siRNA guide strand and a miRNA. Here we evaluate the effect on... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic and Biomolecular Chemistry - November 14, 2017 Category: Molecular Biology Authors: Scott Ryan Suter Source Type: research

siRNA Mediated Silencing of NIN1/RPN12 Binding Protein 1 Homolog Inhibits Proliferation and Growth of Breast Cancer Cells
Authors: Huang WY, Chen DH, Ning L, Wang LW Abstract Retraction: Retracted: siRNA mediated silencing of NIN1/RPN12 binding protein 1 homolog inhibits proliferation and growth of breast cancer cells Asian Pacific Journal of Cancer Prevention has retracted the article titled “siRNA mediated silencing of NIN1/RPN12 binding protein 1 homolog inhibits proliferation and growth of breast cancer cells”(1) for reason of similarity with a series of articles identified by Byrne and Labbé (2). 1. Huang WY1, Chen DH, Ning L, Wang LW. siRNA mediated silencing of NIN1/RPN12 binding protein 1 homolog inhibits proliferation ...
Source: Asian Pacific Journal of Cancer Prevention - November 14, 2017 Category: Cancer & Oncology Tags: Asian Pac J Cancer Prev Source Type: research

Targeted iron nanoparticles with platinum-(IV) prodrugs and anti-EZH2 siRNA show great synergy in combating drug resistance in  vitro and in vivo.
Targeted iron nanoparticles with platinum-(IV) prodrugs and anti-EZH2 siRNA show great synergy in combating drug resistance in vitro and in vivo. Biomaterials. 2017 Nov 14;155:112-123 Authors: Yu C, Ding B, Zhang X, Deng X, Deng K, Cheng Z, Xing B, Jin D, Ma P, Lin J Abstract Resistance to platinum agents is challenging in cancer treatment with platinum drugs. Such resistant cells prevent effective platinum accumulation intracellular and alter cellular adaptations to survive from cytotoxicity by regulating corresponding proteins expression. Ideal therapeutics should combine resolution to these pump ...
Source: Biomaterials - November 14, 2017 Category: Materials Science Authors: Yu C, Ding B, Zhang X, Deng X, Deng K, Cheng Z, Xing B, Jin D, Ma P, Lin J Tags: Biomaterials Source Type: research

Carbonate apatite nanoparticles carry siRNA(s) targeting growth factor receptor genes egfr1 and erbb2 to regress mouse breast tumor.
Authors: Tiash S, Kamaruzman NIB, Chowdhury EH Abstract Cancer cells lose their control on cell cycle by numerous genetic and epigenetic alterations. In a tumor, these cells highly express growth factor receptors (GFRs), eliciting growth, and cell division. Among the GFRs, epidermal growth factor receptor-1 (EGFR1) (Her1/ERBB1) and epidermal growth factor receptor-2 (EGFR2) (Her2/ERBB2) from epidermal growth factor (EGF) family and insulin-like growth factor-1 receptor (IGF1R) are highly expressed on breast cancer cells, thus contributing to the aggressive growth and invasiveness, have been focused in this study. M...
Source: Drug Delivery - November 10, 2017 Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research

Recent advances in galactose-engineered nanocarriers for the site-specific delivery of siRNA and anticancer drugs.
Abstract Galactosylated nanocarriers have recently emerged as viable and versatile tools to deliver drugs at an optimal rate specifically to their target tissues or cells, thus maximizing their therapeutic benefits while circumventing off-target effects. The abundance of lectin receptors on cell surfaces makes the galactosylated carriers suitable for the targeted delivery of bioactives. Additionally, tethering of galactose (GAL) to various carriers, including micelles, liposomes, and nanoparticles (NPs), might also be appropriate for drug delivery. Here, we review recent advances in the development of galactosylat...
Source: Drug Discovery Today - November 9, 2017 Category: Drugs & Pharmacology Authors: Jain A, Jain A, Parajuli P, Mishra V, Ghoshal G, Singh B, Shivhare US, Katare OP, Kesharwani P Tags: Drug Discov Today Source Type: research

siRNA rescues nonhuman primates from advanced Marburg and Ravn virus disease
Ebolaviruses and marburgviruses belong to the family Filoviridae and cause high lethality in infected patients. There are currently no licensed filovirus vaccines or antiviral therapies. The development of broad-spectrum therapies against members of the Marburgvirus genus, including Marburg virus (MARV) and Ravn virus (RAVV), is difficult because of substantial sequence variability. RNAi therapeutics offer a potential solution, as identification of conserved target nucleotide sequences may confer activity across marburgvirus variants. Here, we assessed the therapeutic efficacy of lipid nanoparticle (LNP) delivery of a sing...
Source: Journal of Clinical Investigation - November 7, 2017 Category: Biomedical Science Authors: Emily P. Thi, Chad E. Mire, Amy C.H. Lee, Joan B. Geisbert, Raul Ursic-Bedoya, Krystle N. Agans, Marjorie Robbins, Daniel J. Deer, Robert W. Cross, Andrew S. Kondratowicz, Karla A. Fenton, Ian MacLachlan, Thomas W. Geisbert Source Type: research

Anti-oncogenic activities of cyclin D1b siRNA on human bladder cancer cells via induction of apoptosis and suppression of cancer cell stemness and invasiveness.
Abstract The human cyclin D1 gene generates two major isoforms, cyclin D1a and cyclin D1b, by alternative splicing. Although cyclin D1b mRNA is hardly expressed in normal human tissues, it is detected in approximately 60% of human bladder cancer tissues and cell lines. In the present study, to assess the therapeutic ability of cyclin D1b siRNA, we investigated the anti-oncogenic effects of cyclin D1b siRNA on human bladder cancer cell lines, SBT31A and T24, which express cyclin D1b mRNA. Knockdown of cyclin D1b by specific siRNA significantly suppressed cell proliferation, in vitro cell invasiveness and th...
Source: International Journal of Oncology - November 7, 2017 Category: Cancer & Oncology Authors: Kim CJ, Terado T, Tambe Y, Mukaisho KI, Sugihara H, Kawauchi A, Inoue H Tags: Int J Oncol Source Type: research

Amphiphilic poly( α)glutamate polymeric micelles for systemic administration of siRNA to tumors
RNAi therapeutics carried a great promise to the area of personalized medicine: the ability to target “undruggable” oncogenic pathways. Nevertheless, their efficient tumor targeting via systemic administration had not been resolved yet. Amphiphilic alkylated poly(α)glutamate amine (APA) can serve as a cationic carrier to the negatively-charged oligonucleotides. APA polymers complexed with siRNA to form round-shaped, homogenous and reproducible nano-sized polyplexes bearing ~50nm size and slightly negative charge.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - November 7, 2017 Category: Nanotechnology Authors: Adva Krivitsky, Dina Polyak, Anna Scomparin, Shay Eliyahu, Paula Ofek, Galia Tiram, Hagar Kalinski, Sharon Avkin-Nachum, Nat àlia Feiner Gracia, Lorenzo Albertazzi, Ronit Satchi-Fainaro Source Type: research

Suppression of p53R2 gene expression with specific siRNA sensitizes HepG2 cells to doxorubicin.
CONCLUSION: These findings suggest that siRNA-mediated silencing of p53R2 has great potential as a therapeutic tool and adjuvant in chemotherapy. PMID: 29126924 [PubMed - as supplied by publisher]
Source: Gene - November 7, 2017 Category: Genetics & Stem Cells Authors: Azimi A, Majidinia M, Shafiei-Irannejad V, Jahanban-Esfahlan R, Ahmadi Y, Karimian A, Mir SM, Karami H, Yousefi B Tags: Gene Source Type: research

Amphiphilic poly( α)glutamate polymeric micelles for systemic administration of siRNA to tumors
Publication date: Available online 7 November 2017 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Adva Krivitsky, Dina Polyak, Anna Scomparin, Shay Eliyahu, Paula Ofek, Galia Tiram, Hagar Kalinski, Sharon Avkin-Nachum, Natàlia Feiner Gracia, Lorenzo Albertazzi, Ronit Satchi-Fainaro RNAi therapeutics carried a great promise to the area of personalized medicine: the ability to target “undruggable” oncogenic pathways. Nevertheless, their efficient tumor targeting via systemic administration had not been resolved yet. Amphiphilic alkylated poly(α)glutamate amine (APA) can serve as a cationic carrie...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - November 7, 2017 Category: Nanotechnology Source Type: research

Sequential therapy with redox-responsive glucolipid nanocarrier separately delivering siRNA and doxorubicin to overcome multidrug resistance
Publication date: 20 December 2017 Source:International Journal of Pharmaceutics, Volume 534, Issues 1–2 Author(s): Tingting Meng, Binbin Lu, Shihong Shao, Ming Yuan, Xuan Liu, Hong Yuan, Xuan Huang, Fuqiang Hu P-glycoprotein (P-gp) is a major efflux transporter overexpressed on multidrug resistant tumor cells and responsible for pumping drugs out. If anti-tumor drugs are given when P-gp level is low, satisfactory treatment efficiency may be achieved. Thus, a P-gp down-regulating siRNA (siMDR1) and doxorubicin (DOX) were applied to eliminate multidrug resistant breast cancer cells (MCF-7/ADR). A redox-responsive glucoli...
Source: International Journal of Pharmaceutics - November 6, 2017 Category: Drugs & Pharmacology Source Type: research

A self-assembled polyjuglanin nanoparticle loaded with doxorubicin and anti-Kras siRNA for attenuating multidrug resistance in human lung cancer.
Abstract Lung cancer is a leading cause of cancer-associated mortality worldwide, which has a low survival rate. Multidrug resistance (MDR) is a major obstacle that hinders the treatment of lung cancer. Doxorubicin (DOX) is an anthracycline glycoside antibiotic, having a broad spectrum of anticancer activity against various solid tumors. Juglanin is a natural production, mainly extracted from green walnut husks of Juglans mandshurica, exhibiting various bioactivities. Here, we demonstrated that the combination of drug, gene and nanoparticle overcame MDR, inhibiting lung cancer progression. A novel nanoparticular p...
Source: Biochemical and Biophysical Research communications - October 31, 2017 Category: Biochemistry Authors: Wen ZM, Jie J, Zhang Y, Liu H, Peng LP Tags: Biochem Biophys Res Commun Source Type: research

CD-PLLD co-delivering docetaxel and MMP-9 siRNA plasmid for nasopharyngeal carcinoma therapy in  vivo.
CD-PLLD co-delivering docetaxel and MMP-9 siRNA plasmid for nasopharyngeal carcinoma therapy in vivo. Mol Med Rep. 2017 Jun 07;: Authors: Liu T, Wu X, Wang Y, Hou X, Jiang G, Wu T, Xie H, Xie M Abstract The co-delivery of a drug and a target gene has become a primary strategy in cancer therapy. Based on our previous study, a synthesized star‑shaped co‑polymer consisting of β‑cyclodextrin (CD) and a poly(L‑lysine) dendron (PLLD) was used to co-deliver docetaxel (DOC) and matrix metalloproteinase 9 (MMP‑9) small interfering RNA, via CD‑PLLD/DOC/MMP‑9 complexes, into mice implanted with HN...
Source: Molecular Medicine Reports - October 29, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Systemic Delivery of siRNA by DNA Nanoparticles Targeting Bcl2 in Cancer Cell Growth.
This study suggests that DNPs are effective tools for systemic delivery of therapeutic siRNA and have great potential for further clinical translation. PMID: 29076273 [PubMed - as supplied by publisher]
Source: Angewandte Chemie - October 26, 2017 Category: Chemistry Authors: Rahman MA, Wang P, Zhao Z, Wang D, Nannapaneni S, Zhang C, Chen Z, Griffith CC, Hurwitz SJ, Chen ZG, Ke Y, Shin DM Tags: Angew Chem Int Ed Engl Source Type: research

Self-assembled Polypeptide Nanogels with Enzymatically Transformable Surface as an siRNA Delivery Platform.
Abstract Nanometer-size gel particles, or nanogels, have potential for delivering therapeutic macromolecules. A cationic surface promotes cellular internalization of nanogels but undesired electrostatic interactions, such as with blood components, cause instability and toxicities. Polyethylene glycol coating has been used to shield charges, but this decreases delivery efficiency. Technical difficulties in synthesis and controlling molecular weights make it unfeasible to, instead, coat with biodegradable polymers. Our proposed solution is cationized nanogels enzymatically functionalized with branched polysaccharide...
Source: Biomacromolecules - October 23, 2017 Category: Biochemistry Authors: Nishimura T, Yamada A, Umezaki K, Sawada SI, Mukai SA, Sasaki Y, Akiyoshi K Tags: Biomacromolecules Source Type: research

Glycosylated RAFT polymers with varying PEG linkers produce different siRNA uptake, gene silencing and toxicity profiles.
Abstract Achieving efficient and targeted delivery of short interfering (siRNA) is an important research challenge to overcome to render highly promising siRNA therapies clinically successful. Challenges exist in designing synthetic carriers for these RNAi constructs that provide protection against serum degradation, extended blood retention times, effective cellular uptake through a variety of uptake mechanisms, endosomal escape and efficient cargo release. These challenges have resulted in a significant body of research, and led to many important findings about the chemical composition and structural layout of t...
Source: Biomacromolecules - October 23, 2017 Category: Biochemistry Authors: Williams EGL, Hutt OE, Hinton TM, Larnaudie SC, Le T, MacDonald JM, Gunatillake P, Thang SH, Duggan PJ Tags: Biomacromolecules Source Type: research

Suppression of LETM1 by siRNA inhibits cell proliferation and invasion of bladder cancer cells.
Authors: Huang B, Zhang J, Zhang X, Huang C, Hu G, Li S, Xie T, Liu M, Xu Y Abstract The leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) is highly expressed in many human malignancies and is correlated with poor prognosis. However, the function of LETM1 in bladder cancer still remains unknown. In the present study, we analyzed the expression levels of LETM1 in bladder cancer tissues and non-cancerous tissues as well as in four bladder cancer cell lines (T24, EJ, 5637 and J82) and a human bladder epithelial immortalized cell line SV-HUC-1. Small interfering RNA (siRNA) was employed to knockdow...
Source: Oncology Reports - October 20, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Co ‑expression of murine double minute 2 siRNA and wild‑type p53 induces G1 cell cycle arrest in H1299 cells.
Co‑expression of murine double minute 2 siRNA and wild‑type p53 induces G1 cell cycle arrest in H1299 cells. Mol Med Rep. 2017 Oct 11;: Authors: Liu L, Zhang P, Guo H, Tang X, Liu L, Li J, Guo R, Cai Y, Liu Y, Li Y Abstract The therapeutic options available for the treatment of advanced non-small cell lung cancer have increased over the past decade. Small molecule gene therapy has emerged as an effective therapy for the treatment of lung cancer in vitro and in vivo although it has not been tested in a clinical setting. In particular, therapies that target the negative feedback loop between p53 an...
Source: Molecular Medicine Reports - October 20, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Anti-tumor activity of KNTC2 siRNA in orthotopic tumor model mice of hepatocellular carcinoma.
Abstract Hepatocellular carcinoma (HCC) is still one of the major causes of cancer-related death. Kinetochore-associated protein 2 (KNTC2) is specifically upregulated in tumor tissues of HCC patients and recognized as a potential candidate target for the treatment of HCC. However, the relationship between KNTC2 and in vivo tumor growth of HCC is not yet fully understood. Here we encapsulated KNTC2 siRNAs into a lipid nanoparticle (LNP) and investigated their knockdown activity, target engagement marker, anti-tumor activity and hepatotoxicity in an orthotopic HCC model mice of Hep3B-luc cells. Single i.v. administ...
Source: Biochemical and Biophysical Research communications - October 18, 2017 Category: Biochemistry Authors: Makita Y, Murata S, Katou Y, Kikuchi K, Uejima H, Teratani M, Hoashi Y, Kenjo E, Matsumoto S, Nogami M, Otake K, Kawamata Y Tags: Biochem Biophys Res Commun Source Type: research

Treatment of pulmonary fibrosis with siRNA against a collagen-specific chaperone HSP47 in vitamin A-coupled liposomes.
CONCLUSIONS: These results suggest that VA-lip-siRNA HSP47 improves pulmonary fibrosis in not only preventive, but also therapeutic schedules, and thus, this drug delivery system should provide a novel therapy for refractory pulmonary fibrosis. PMID: 29035148 [PubMed - as supplied by publisher]
Source: Experimental Lung Research - October 17, 2017 Category: Respiratory Medicine Tags: Exp Lung Res Source Type: research

Lipid-based DNA/siRNA transfection agents disrupt neuronal bioenergetics and mitophagy.
Abstract A multitude of natural and artificial compounds have been recognized to modulate autophagy, providing direct or, through associated pathways, indirect entry points to activation and inhibition. While these pharmacological tools are extremely useful in the study of autophagy, their abundance also suggests the potential presence of unidentified autophagic modulators that may interfere with experimental designs if applied unknowingly. Here, we report unanticipated effects on autophagy and bioenergetics in neuronal progenitor cells (NPC) incubated with widely used lipid-based-transfection reagent lipofectamin...
Source: The Biochemical Journal - October 12, 2017 Category: Biochemistry Authors: Napoli E, Liu S, Marsilio I, Zarbalis K, Giulivi C Tags: Biochem J Source Type: research

SiRNA directed against NF ‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro.
SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro. Mol Med Rep. 2017 Oct 04;: Authors: Wu C, Zhao J, Zhu G, Huang Y, Jin L Abstract Acute lung injury (ALI) is a condition of acute respiratory failure, characterized by diffuse pulmonary infiltrates and severe hypoxemia. During ALI, the acute phase of inflammation induces the recruitment of activated inflammatory cells, including macrophages and lymphocytes, to the damaged lesions. Nuclear factor (NF)‑κB is a key protein in many signal transduction pathways, over‑activation of which i...
Source: Molecular Medicine Reports - October 11, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Stealth magnetic nanocarriers of siRNA as platform for breast cancer theranostics
Publication date: 5 November 2017 Source:International Journal of Pharmaceutics, Volume 532, Issue 2 Author(s): J. Bruniaux, S. Ben Djemaa, K. Hervé-Aubert, H. Marchais, I. Chourpa, S. David The endogenous mechanism of RNA interference is more and more used in research to obtain specific down-regulation of gene expression in diseases such as breast cancer. Currently, despite the new fields of study open up by RNA interference, the rapid degradation of siRNA by nucleases and their negative charges prevent them from crossing cell membranes. To overcome these limitations, superparamagnetic iron oxide nanoparticles (SPIONs) ...
Source: International Journal of Pharmaceutics - October 11, 2017 Category: Drugs & Pharmacology Source Type: research

Co-Administration of Myostatin-Targeting siRNA and ActRIIB-Fc Fusion Protein Increases Masseter Muscle Mass and Fiber Size.
Authors: Bayarsaikhan O, Kawai N, Mori H, Kinouchi N, Nikawa T, Tanaka E Abstract Myostatin, a member of the TGF-β superfamily, is a negative regulator of skeletal muscle cell growth and differentiation, and binds with high affinity to the activin type IIB receptor (ActRIIB). The soluble ligand-binding domain of ActRIIB fused to the Fc domain of IgG (ActRIIB-Fc) potently binds and inhibits TGF-β family members in muscle, leading to rapid and marked muscle growth. The present study was designed to assess the effectiveness of the co-delivery of myostatin-targeting siRNA (Mstn-siRNA) and ActRIIB-Fc into skeletal mus...
Source: Journal of Nutritional Science and Vitaminology - October 7, 2017 Category: Nutrition Tags: J Nutr Sci Vitaminol (Tokyo) Source Type: research

Identifying HIPK1 as target of miR-22-3p enhancing recombinant protein production from HEK 293 cell by using microarray and HTP siRNA screen.
Abstract Protein expression from human embryonic kidney cells (HEK 293) is an important tool for structural and clinical studies. It was previously shown that microRNAs (small, noncoding RNAs) are effective means for improved protein expression from these cells, and by conducting a high-throughput screening of the human microRNA library, several microRNAs were identified as potential candidates for improving expression. From these, miR-22-3p was chosen for further study since it increased the expression of luciferase, two membrane proteins and a secreted fusion protein with minimal effect on the cells' growth and ...
Source: Biotechnology Journal - October 7, 2017 Category: Biotechnology Authors: Inwood S, Buehler E, Betenbaugh M, Lal M, Shiloach J Tags: Biotechnol J Source Type: research

Targeted inhibition of Hantavirus replication and intracranial pathogenesis by a chimeric protein-delivered siRNA.
Abstract Hantavirus (HV) infection, which underlies hantavirus hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome, remains to be a severe clinical challenge. Here, we synthesized small interfering RNAs (siRNAs) that target the encoding sequences of HV strain 76-118, and validated their inhibitory role in virus replication in HV-infected monkey kidney Vero E6 cells. A chimeric protein, 3G1-Cκ-tP, consisting of a single-chain antibody fragment (3G1) against the HV surface envelop glycoprotein, the constant region of human immunoglobulin κ chain (Cκ), and truncated protamine (amino acids 8-29,...
Source: Antiviral Research - October 7, 2017 Category: Virology Authors: Yang J, Sun JF, Wang TT, Guo XH, Wei JX, Jia LT, Yang AG Tags: Antiviral Res Source Type: research

GABAB receptor ligand-directed trimethyl chitosan/tripolyphosphate nanoparticles and their pMDI formulation for survivin siRNA pulmonary delivery
Publication date: 1 January 2018 Source:Carbohydrate Polymers, Volume 179 Author(s): Suhui Ni, Yun Liu, Yue Tang, Jing Chen, Shuhan Li, Ji Pu, Lidong Han The effect of gene silencing by survivin siRNA (siSurvivin) on the proliferation and apoptosis of lung tumor has been attracted more interest. GABAB receptor ligand-directed nanoparticles consisting of baclofen functionalized trimethyl chitosan (Bac-TMC) as polymeric carriers, tripolyphosphate (TPP) as ionic crosslinker, and siSurvivin as therapeutic genes, were designed to enhance the survivin gene silencing. GABAB receptor agonist baclofen (Bac) was initially introduce...
Source: Carbohydrate Polymers - October 2, 2017 Category: Biomedical Science Source Type: research

Genome-wide siRNA screen identifies UNC50 as a regulator of Shiga toxin 2 trafficking
Shiga toxins 1 and 2 (STx1 and STx2) undergo retrograde trafficking to reach the cytosol. Early endosome-to-Golgi transport allows the toxins to evade degradation in lysosomes. Targeting this trafficking step has therapeutic promise, but the mechanism of trafficking for the more potent toxin STx2 is unclear. To identify host factors required for early endosome-to-Golgi trafficking of STx2, we performed a viability-based genome-wide siRNA screen in HeLa cells. 564, 535, and 196 genes were found to be required for toxicity induced by STx1 only, STx2 only, and both toxins, respectively. We focused on validating endosome/Golgi...
Source: Journal of Cell Biology - October 2, 2017 Category: Cytology Authors: Selyunin, A. S., Iles, L. R., Bartholomeusz, G., Mukhopadhyay, S. Tags: Disease, Trafficking, Microbiology Articles Source Type: research

Diblock copolymer hydrophobicity facilitates efficient gene silencing and cytocompatible nanoparticle-mediated siRNA delivery to musculoskeletal cell types.
Abstract pH-responsive diblock copolymers provide tailorable nanoparticle (NP) architecture and chemistry critical for siRNA delivery. Here, diblock polymers varying in first (corona) and second (core) block molecular weight (Mn), Corona:Core ratio and core hydrophobicity (%BMA) were synthesized to determine their effect on siRNA delivery in murine tenocytes (mTenocyte) and murine and human mesenchymal stem cells (mMSC and hMSCs, respectively). NP-mediated siRNA uptake, gene silencing, and cytocompatibility were quantified. Uptake is positively correlated with first block Mn in mTenocytes and hMSCs (p≤0.0005). A...
Source: Biomacromolecules - September 29, 2017 Category: Biochemistry Authors: Malcolm DW, Freeberg MAT, Wang Y, Sims KR, Awad HA, Benoit DSW Tags: Biomacromolecules Source Type: research