Expression and function of FRA1 protein in tumors.
Abstract AP-1 is a dimeric complex that is composed of JUN, FOS, ATF and MAF protein families. FOS-related antigen 1 (FRA1) which encoded by FOSL1 gene, belongs to the FOS protein family, and mainly forms an AP-1 complex with the protein of the JUN family to exert an effect. Regulation of FRA1 occurs at levels of transcription and post-translational modification, and phosphorylation is the major post-translational modification. FRA1 is mainly regulated by the mitogen-activated protein kinases signaling pathway and is degraded by ubiquitin-independent proteasomes. FRA1 can affect biological functions, such as tumor...
Source: Molecular Biology Reports - October 14, 2019 Category: Molecular Biology Authors: Jiang X, Xie H, Dou Y, Yuan J, Zeng D, Xiao S Tags: Mol Biol Rep Source Type: research

A comparative assessment of phenotypic and molecular diversity in Doum (Hyphaene thebaica L.).
Abstract Doum palm (Hyphaene thebaica L.) has been reported as one of the most beneficial species worldwide. Its fruits are a good source of fibers, antioxidants, B-complex vitamins, essential minerals, monosaccharides, essential oil and flavonoids. Fruit extract shows anti-inflammatory, antioxidant, antimicrobial, anticancer and pharmacological potential. However, there is a lack of investigations dealing with its genetic diversity. Therefore, a collection of twelve mature female palm landraces grown in the botanical garden of Aswan, "Egypt" were used for genetic diversity assessment based on phenotypic...
Source: Molecular Biology Reports - October 14, 2019 Category: Molecular Biology Authors: Khalil OA, Ibrahim RA, Youssef M Tags: Mol Biol Rep Source Type: research

The integrative regulatory network of circRNA and microRNA in keloid scarring.
This study revealed that circRNAs are potentially implicated in the development of keloid and could serve as novel diagnostic and therapeutic targets. PMID: 31612410 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 14, 2019 Category: Molecular Biology Authors: Shi J, Yao S, Chen P, Yang Y, Qian M, Han Y, Wang N, Zhao Y, He Y, Lyu L, Lu D Tags: Mol Biol Rep Source Type: research

The cellular and molecular basis of major depressive disorder: towards a unified model for understanding clinical depression.
Abstract Major depressive disorder (MDD) is considered a serious public health issue that adversely impacts an individual's quality of life and contributes significantly to the global burden of disease. The clinical heterogeneity that exists among patients limits the ability of MDD to be accurately diagnosed and currently, a symptom-based approach is utilized in many cases. Due to the complex nature of this disorder, and lack of precise knowledge regarding the pathophysiology, effective management is challenging. The aetiology and pathophysiology of MDD remain largely unknown given the complex genetic and environm...
Source: Molecular Biology Reports - October 14, 2019 Category: Molecular Biology Authors: Pitsillou E, Bresnehan SM, Kagarakis EA, Wijoyo SJ, Liang J, Hung A, Karagiannis TC Tags: Mol Biol Rep Source Type: research

Chemical screening identifies an extract from marine Pseudomonas sp.-PTR-08 as an anti-aging agent that promotes fission yeast longevity by modulating the Pap1-ctt1+ pathway and the cell cycle.
Abstract Aging is a degenerative process characterized by progressive deterioration of cellular components, ultimately resulting in mortality, in which massive accumulation of reactive oxygen species (ROS) and advanced glycation end products (AGEs) are implicated as crucial factors. At the same time, natural products are rich sources from which to isolate and characterize potential anti-aging compounds. The current study was designed to extract compounds from the marine bacterium Pseudomonas sp. and investigate their in vitro antioxidant and anti-glycation activities, as well as their in vivo effects on aging in t...
Source: Molecular Biology Reports - October 14, 2019 Category: Molecular Biology Authors: Prastya ME, Astuti RI, Batubara I, Takagi H, Wahyudi AT Tags: Mol Biol Rep Source Type: research

Gene expression changes and promoter methylation with the combined effects of estradiol and leptin in uterine tissue of the ovariectomized mice model of menopause.
In conclusion, our in vivo studies determined specific gene expression and signaling protein changes, and further unraveled the molecular targets of estradiol + leptin that may perturb endometrial homeostasis and lead to endometrial hyperplasia development in the chronic stimulated state. PMID: 31602590 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 10, 2019 Category: Molecular Biology Authors: Shetty A, Venkatesh T, Tsutsumi R, Suresh PS Tags: Mol Biol Rep Source Type: research

Upregulation of secreted aspartyl proteinase genes of fluconazole-sensitive Candida albicans isolates.
This study investigated the influence of inhibitory and sub-inhibitory fluconazole concentrations on Sap activity and their gene transcription for three clinical C. albicans isolates. Two isolates presented significant increases in Sap activity and transcription of SAP 1-8 genes in the presence of 1 MIC80 of fluconazole compared to the absence of the antifungal agent. The results suggest that the increase in Sap activity occurs due to an upregulation of the SAP gene transcription influenced by fluconazole. This suggests the importance of all SAP genes in the progression of bloodstream infections compared to primary tissue ...
Source: Molecular Biology Reports - October 10, 2019 Category: Molecular Biology Authors: do Rosário Esteves Guimarães C, de Freitas HF, Barros TF Tags: Mol Biol Rep Source Type: research

Evaluating the genetic structure of wild and commercial red cusk-eel (Genypterus chilensis) populations through the development of novel microsatellite markers from a reference transcriptome.
The objective of this study was to develop a set of microsatellite markers, estimate genetic parameters, determine population differentiation, and identify the population structure of wild and commercial populations of G. chilensis. We discovered 6427 microsatellites markers from RNA-seq data, of which 54.9%, 20.2% and 16.8% were di-, tri-, and tetranucleotides, respectively. We used 12 of these markers to genotype two sets of broodstock, one group from commercial fish, and one group from wild fish from the Coquimbo Region of G. chilensis. We estimate the genetic parameters of the markers, selecting ten polymorphic markers...
Source: Molecular Biology Reports - October 9, 2019 Category: Molecular Biology Authors: González P, Dettleff P, Valenzuela C, Estrada JM, Valdés JA, Meneses C, Molina A Tags: Mol Biol Rep Source Type: research

Psychiatric disorders and SLC6A4 gene variants: possible effects on alcohol dependence and alzheimer's disease.
In this study we aimed to evaluate the pleiotropic nature of SLC6A4 alterations and their association with the overall risk of brain diseases rather than disorder-specific. SLC6A4 variants, namely 5HTTLPR, STin2, rs2066713, rs25531, rs4251417, rs6354 and rs7224199 were investigated in 4 independent cohorts of subjects with specific psychiatric disorders, including Alcohol dependence disorder (ALC), Alzheimer disease (ALZ), Schizophrenia (SCZ) and Bipolar disorder (BPD). Other variables (biochemical parameters and Psychiatric scales scores) were also tested for association. SLC6A4 polymorphisms are not associated with the r...
Source: Molecular Biology Reports - October 8, 2019 Category: Molecular Biology Authors: Calabrò M, Mandelli L, Crisafulli C, Porcelli S, Albani D, Politis A, Papadimitriou GN, Di Nicola M, Janiri L, Colombo R, Martinotti G, Bellomo A, Vieta E, Bonassi S, Frustaci A, Ducci G, Landi S, Boccia S, Serretti A Tags: Mol Biol Rep Source Type: research

BACs-on-Beads ™ assay, a rapid aneuploidy test, improves the diagnostic yield of conventional karyotyping.
This study was conducted to appraise the usefulness of the BoB™ assay as a complementary diagnostic tool to conventional karyotyping for the rapid detection of chromosomal aneuploidies. A total of 485 prenatal (amniotic fluid and chorionic villi) and blood/products of conception samples were collected between July 2013 and August 2018, and analyzed by the BoBs™ assay and cytogenetic karyotyping and further validated by fluorescence in situ hybridization (FISH). Forty-three of 484 qualifying samples (8.9%) were identified as abnormal by the BoBs™ assay. The assay was comparable to karyotyping in the detect...
Source: Molecular Biology Reports - October 8, 2019 Category: Molecular Biology Authors: Farra C, Nassar AH, Mirza F, Abdouni L, Souaid M, Awwad J Tags: Mol Biol Rep Source Type: research

A potential marker in brucellosis, long non coding RNA IFNG-AS1.
In this study, associations of lncRNA IFNG-AS1 expression with secretion of IFN-γ level in Sixty patients with brucellosis, which were divided into 3 groups (acute, chronic and relapse groups), as a case group were compared with 20 subjects with negative serological tests and brucellosis clinical manifestation as a control group. In this regard, RNA were extracted from isolated peripheral blood mononuclear cells (PBMCs). LncRNA IFNG-AS1, T-box transcription factor (T-bet) and IFN-γ expressions were detected using quantitative polymerase chain reaction (qPCR). Serum level IFN-γ was assessed using enzyme li...
Source: Molecular Biology Reports - October 8, 2019 Category: Molecular Biology Authors: Gheitasi R, Jourghasemi S, Pakzad I, Hosseinpour Sarmadi V, Samieipour Y, Sekawi Z, Azizi Jalilian F Tags: Mol Biol Rep Source Type: research

Evaluation of the frequency of CD5+ B cells as natural immunoglobulin M producers and circulating soluble CD5 in patients with bladder cancer.
Abstract Bladder cancer is the most common malignancy of the genitourinary tract. It is the fourth most common malignancy in men and the fifth most common malignancy in the general population, with a high recurrence rate. CD5+ B lymphocytes are a subset of B lymphocytes, which contribute to innate immune responses. These cells are involved in the spontaneous production of self-reactive natural antibodies. On the other hand, natural antibodies can recognize tumor-associated antigens, including proteins or carbohydrates, and eliminate these cells in a complement-dependent manner or via induction of apoptosis. Beside...
Source: Molecular Biology Reports - October 5, 2019 Category: Molecular Biology Authors: Roudafshani Z, Jazayeri MH, Mahmoudi AR, Nedaeinia R, Safari E, Jazayeri A Tags: Mol Biol Rep Source Type: research

Propidium monoazide-polymerase chain reaction for detection of residual periprosthetic joint infection in two-stage revision.
This study aimed to determine the role of PMA in PCR for diagnosis of PJI. Clinical samples were tested by PCR with and without prior treatment with PMA and compared to conventional microbiological culture. The PCR assay included genus-specific primers for staphylococci and enterococci and species-specific primers for Cutibacterium acnes. The validated conditions of PMA treatment used in this study were 20 μM concentration and 5 and 10 min of dark incubation and photo-activation respectively. 202 periprosthetic tissues and explanted prostheses from 60 episodes in 58 patients undergoing revision arthroplasties ...
Source: Molecular Biology Reports - October 5, 2019 Category: Molecular Biology Authors: Askar M, Sajid M, Nassif Y, Ashraf W, Scammell B, Bayston R Tags: Mol Biol Rep Source Type: research

CASP8 (rs3834129) and CASP3 (rs4647601) polymorphisms in oropharynx cancer risk, tumor cell differentiation, and prognosis in a cohort of the Brazilian population.
The objective of this research was to assess the association of genetic polymorphisms related to intrinsic apoptosis pathway CASP8 rs3834129 and CASP3 rs4647601 with the risk, clinical and pathological aspects, and survival of oropharynx squamous cell carcinoma (OPSCC) patients that received cisplatin and radiotherapy. The genotypes were identified in 198 patients with OPSCC and 200 controls using polymerase chain reaction methods. Chi square or Fisher's exact test and logistic regression were applied for the detection of differences between groups. Patients' genotypes were statistically evaluated considering the event-fre...
Source: Molecular Biology Reports - October 5, 2019 Category: Molecular Biology Authors: Tortorelli GA, Torricelli C, Carron J, Costa EFD, Lopes-Aguiar L, Carvalho BF, Rinck-Junior JA, Mariano FV, Altemani AMAM, Lima CSP, Lourenço GJ Tags: Mol Biol Rep Source Type: research

Genetic diversity and karyotype of Pitcairnia azouryi: an endangered species of Bromeliaceae endemic to Atlantic Forest inselbergs.
jo TT Abstract Plant species of various families, such as those of Bromeliaceae, occur on inselbergs where they are subject to geographic isolation and environmental conditions that can lead to genetic erosion. This, in turn, can result in the loss of natural populations due to homozygosis, or changes in ploidy that may lead to reproductive isolation. The genetic diversity of five natural populations of Pitcairnia azouryi was measured using nine microsatellite markers transferred from P. albiflos and P. geyskesii. Chromosome numbers and nuclear DNA content were also evaluated. The results indicated moderate geneti...
Source: Molecular Biology Reports - October 5, 2019 Category: Molecular Biology Authors: Manhães VDC, de Miranda FD, Clarindo WR, Carrijo TT Tags: Mol Biol Rep Source Type: research

Inheritance of faba bean resistance to Broomrape, genetic diversity and QTL mapping analysis.
Abstract Six faba bean parents and their F1 and F2 generations were used in this investigation to study the genetic system controlling resistance of faba bean (Vicia faba L.) to broomrape (Orobanche crenata). Most of the F1 hybrids were tolerant to broomrape. In the F2 generation, the population P5 × P6 (Assiut 125 × Romy 12) gave the highest value of relative yield and tolerance index. Heterosis and inbreeding depression were only positive in number of tillers/plant and seed yield/plant characters. The results indicated that the additive effect was more important than the domin...
Source: Molecular Biology Reports - October 4, 2019 Category: Molecular Biology Authors: Abd El-Fatah BES, Nassef DMT Tags: Mol Biol Rep Source Type: research

Interacting adipose-derived stem cells and microvascular endothelial cells provide a beneficial milieu for soft tissue healing.
r M Abstract There is growing evidence suggesting that healing of chronic soft tissue wounds profits from the presence of adipose-derived stem cells (ADSC). Among the large spectrum of mechanisms by which ADSC might act, especially the interaction with the microvascular endothelial cell, a main player during angiogenesis, is of special interest. In the present 2D model on the basis of endothelial cell ADSC co-cultures, we focused on the identification of characteristics of both cell types in response to a typical condition in acute and chronic wounds: hypoxia. Parameters like proliferation capacity, migration, myo...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Bachmann S, Jennewein M, Bubel M, Guthörl S, Pohlemann T, Oberringer M Tags: Mol Biol Rep Source Type: research

Testis-specific Arf promoter expression in a transposase-aided BAC transgenic mouse model.
Abstract CDKN2A is an evolutionarily conserved gene encoding proteins implicated in tumor suppression, ocular development, aging, and metabolic diseases. Like the human form, mouse Cdkn2a encodes two distinct proteins-p16Ink4a, which blocks cyclin-dependent kinase activity, and p19Arf, which is best known as a positive regulator of the p53 tumor suppressor-and their functions have been well-studied in genetically engineered mouse models. Relatively little is known about how expression of the two transcripts is controlled in normal development and in certain disease states. To better understand their coordinate and...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Sung CY, Liu YT, Bennett LB, Devitt CC, Skapek SX Tags: Mol Biol Rep Source Type: research

Efficacy, toxicity study and antioxidant properties of plantaricin E and F recombinants against enteropathogenic Escherichia coli K1.1 (EPEC K1.1).
This study was aimed to evaluate the efficacy and toxicity of plantaricin E and F recombinant against EPEC K1.1 infection by in vivo assay. The production of plantaricin E and F recombinants from Lactococcus lactis was conducted and encapsulated. The in vivo study was carried out by inoculating the mice perorally with EPEC K1.1 for 7 days then treated with 100, 250, and 500 mg/kg body weight/day of recombinant plantaricin E and F for another 7 days. The toxicity assay were observed in ddY mice using various concentrations of treatment (50, 100, 1000, and 5000 mg/kg/body weight) doses perorally for 48&nb...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Hanny ELL, Mustopa AZ, Budiarti S, Darusman HS, Ningrum RA, Fatimah Tags: Mol Biol Rep Source Type: research

Inhibition of DNA-PK potentiates the synergistic effect of NK314 and etoposide combination on human glioblastoma cells.
Abstract Etoposide (VP-16) is the topoisomerase 2 (Top2) inhibitor used for treating of glioma patients however at high dose with serious side effects. It induces DNA double-strand breaks (DSBs). These DNA lesions are repaired by non-homologous DNA end joining (NHEJ) mediated by DNA-dependent protein kinase (DNA-PK). One possible approach to decrease the toxicity of etoposide is to reduce the dose while maintaining the anticancer potential. It could be achieved through combined therapy with other anticancer drugs. We have assumed that this objective can be obtained by (1) a parallel topo2 α inhibition and (2...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Kopa P, Macieja A, Gulbas I, Pastwa E, Poplawski T Tags: Mol Biol Rep Source Type: research

Development and verification of a three-dimensional (3D) breast cancer tumor model composed of circulating tumor cell (CTC) subsets.
Abstract Breast cancer is one of the most common cancer types among women in which early tumor invasion leads to metastases and death. EpCAM (epithelial cellular adhesion molecule) and HER2 (human epidermal growth factor receptor 2) are two main circulating tumor cell (CTC) subsets in HER2+ breast cancer patients. In this regard, the main aim of this study is to develop and characterize a three-dimensional (3D) breast cancer tumor model composed of CTC subsets to evaluate new therapeutic strategies and drugs. For this reason, EpCAM(+) and HER2(+) sub-populations were isolated from different cell lines to es...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Anil-Inevi M, Sağlam-Metiner P, Kabak EC, Gulce-Iz S Tags: Mol Biol Rep Source Type: research

A novel IRF2BPL truncating variant is associated with endolysosomal storage.
Abstract De novo mutations in the IRF2BPL gene have been identified to date in 18 patients presenting with neuromotor regression, epilepsy and variable neurological signs. Here, we report a female child carrying a novel heterozygous truncating variant in IRF2BPL. Following normal development for two and half years, she developed a progressive neurological condition with psychomotor regression, dystonic tetraparesis with hyperkinetic movements, but no overt epilepsy. Skin biopsy revealed enlarged lysosomes containing granular and tubular material, suggestive of a lysosomal storage disorder. This case expands the IR...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Ginevrino M, Battini R, Nuovo S, Simonati A, Micalizzi A, Contaldo I, Serpieri V, Valente EM Tags: Mol Biol Rep Source Type: research

Phenotypic and genotypic methods for identification of slime layer production, efflux pump activity, and antimicrobial resistance genes as potential causes of the antimicrobial resistance of some mastitis pathogens from farms in Menoufia, Egypt.
Abstract Mastitis caused by multi- or pan-drug resistant bacteria is a growing health concern. A total of 110 milk samples were collected: Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Enterococcus faecalis, and Escherichia coli were present in 54/110 (49.09%), 37/110 (33.63%), 25/110 (22.72%), 7/110 (6.36%), and 50/110 (45.45%) samples, respectively. A total of 20 methicillin-resistant S. aureus (MRSA) isolates, 19 Streptococcus sp. isolates, and 15 E. coli isolates were selected, and 100% were positive for (coagulase and hemolysins), streptokinase, and hemolytic activity, respectiv...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Elsayed MSAE, Roshdey T, Salah A, Tarabees R, Younis G, Eldeep D Tags: Mol Biol Rep Source Type: research

Mechanism of CNP-mediated DG-PKC and IP4 signaling pathway in diabetic rats with gastric motility disorder.
Abstract In the precedent research conducted by the same team, it concluded that the activities in C-type natriuretic peptide (CNP)/cyclic guanosine monophosphate (cGMP)/cyclic adenosine monophosphate (cAMP)/β-type phospholipase C (PLCβ) pathways of rat antral smooth muscle were changed due to diabetes, which was the key pathogenetic mechanism for diabetic gastric dysmotility. As the follow-on step, this study was designed to probe into the downstream signaling pathway of CNP/PLCβ. The results showed that level of α-type protein kinase C (PKCα),cell membrane to cytoplasm ratio of PKC&alp...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Lian HM, Guo JY, Sun Y, Zhang MH, Piao LH, Jin Z, Cai YL Tags: Mol Biol Rep Source Type: research

Development and characterization of genomic microsatellite markers in the tree species, Rhodoleia championii, R. parvipetala, and R. forrestii (Hamamelidaceae).
Abstract Rhodoleia Champion ex Hooker is one of the most primitive relict genera of Hamamelidaceae, a key family exploited to understand the origin and early evolution of flowering plants. Genomic simple sequence repeats (SSRs) were developed for R. championii to perform genetic diversity, phylogeographical structure or even systematic evolution studies of the genus. Among the 278,743 contigs (105,758,242 bps) de novo assembled from the low-coverage whole genome sequencing of R. championii, a total of 9106 SSRs were detected in 8370 contigs, and SSR primer pairs were successfully designed for 6677 SSRs. Among the ...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Huang Y, Yin Q, Do VT, Meng K, Chen S, Liao B, Fan Q Tags: Mol Biol Rep Source Type: research

Stable reference genes for expression studies in breast muscle of normal and white striping-affected chickens.
Abstract The normalization with proper reference genes is a crucial step to obtain accurate mRNA expression levels in quantitative PCR (qPCR) studies. Therefore, in this study, 10 reference candidate genes were evaluated to determine their stability in normal pectoralis major muscle of broilers and those counterparts affected with White Striping (WS) myopathy at 42 days age. Four different tools were used for ranking the most stable genes: GeNorm, NormFinder, BestKeeper and Comparative Ct (ΔCt), and a general ranking was performed using the RankAggreg tool to select the best reference genes among all to...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Marciano CMM, Ibelli AMG, Peixoto JO, Savoldi IR, do Carmo KB, Fernandes LT, Ledur MC Tags: Mol Biol Rep Source Type: research

In vitro and in silico anticancer activity of amygdalin on the SK-BR-3 human breast cancer cell line.
Abstract In spite of several studies that have shown the cytotoxic effects of amygdalin on the different cancer cell lines, however, the chemopreventive potential of amygdalin on the breast cancer cell line is not completely understood. We investigated the effect of amygdalin on the cell death and the level of pro-apoptotic Bax protein and anti-apoptotic Bcl-2 protein in SK-BR-3 human breast cancer cell line. The cell viability of SK-BR-3 cells was evaluated by MTT assay in different concentration of amygdalin. The level of Bax and Bcl-2 in SK-BR-3 cells were measured by western blot analysis. For statistical anal...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Moradipoodeh B, Jamalan M, Zeinali M, Fereidoonnezhad M, Mohammadzadeh G Tags: Mol Biol Rep Source Type: research

Functional annotation of mulberry (Morus spp.) transcriptome, differential expression of genes related to growth and identification of putative genic SSRs, SNPs and InDels.
In this study, we have explored the molecular basis underlying growth using Transcriptome profiling of contrasting genotypes. A total of 66.6 Mbp of primary transcriptomes from high growth (HGG)-Jalalgarah-3 and M. laevigata (H) and, low growth genotypes (LGG)-Harmutty and Vadagaraparai-2; resulting in 24210, 27998, 28085 and 28764 final transcripts respectively. Out of the 34096 pooled transcripts, 20249 transcripts matched with at least one sequence of the non-redundant database. Functional annotation resulted in the categorization of 18970 transcripts into 3 gene ontology (GO) terms and 7440 were assigned to 23 Kyoto en...
Source: Molecular Biology Reports - October 3, 2019 Category: Molecular Biology Authors: Rukmangada MS, Sumathy R, Naik VG Tags: Mol Biol Rep Source Type: research

Detection of small numbers of iPSCs in different heterogeneous cell mixtures with highly sensitive droplet digital PCR.
Abstract Induced pluripotent stem cells (iPS cells) are a prospective resource for regenerative biomedicine. iPS cells can differentiate into any type of stem, progenitor and somatic cells to help replace structures within damaged organs or tissues. However, iPS cells themselves, can produce malignant tumors if they are injected into the body of an immunocompatible or immunodeficient recipient. Thus, it is necessary to detect any residual iPS cells content in biomedical cell products obtained from iPS cells and destined for transplantation. In this article we describe searches for iPS cells in heterogeneous cell m...
Source: Molecular Biology Reports - October 1, 2019 Category: Molecular Biology Authors: Artyuhov AS, Dashinimaev EB, Mescheryakova NV, Ashikhmina AA, Vorotelyak EA, Vasiliev AV Tags: Mol Biol Rep Source Type: research

Non-synonymous polymorphisms in candidate gene associated with growth traits in Channel catfish (Ictalurus punctatus, Rafinesque, 1818).
la Rosa-Reyna XF Abstract Resequencing of Myostatin, Growth Hormone, Follistatin-A-like, Insulin-like Growth Factor I (IGF-I) and Myogenin (MYOG) genes was completed to discover novel genetic variations and assess non synonymous (ns) polymorphisms (SNPs) effect on growth related traits of channel catfish. Wild and farmed animals were used as a discovering population. Resequencing lead to the identification of 59 new variants in the five analyzed genes; 66% found in introns and 34% in coding regions. From coding regions, 14 variants were synonyms and six were ns variations. A mutation rate of one in 129 bp wa...
Source: Molecular Biology Reports - October 1, 2019 Category: Molecular Biology Authors: Suárez-Salgado D, Parra-Bracamonte GM, Benavides-González F, Alfaro IOM, Rincón AMS, Moreno-Medina VR, De la Rosa-Reyna XF Tags: Mol Biol Rep Source Type: research

L-Arginine alleviates heat stress-induced intestinal epithelial barrier damage by promoting expression of tight junction proteins via the AMPK pathway.
This study tested the hypothesis that L-arginine regulates the TJ network by activating AMP-activated protein kinase (AMPK) signaling, which in turn improves intestinal barrier functions under HS. IEC-6 cells and rat small intestines were used as experiment models of heat stress. AICAR and dorsomorphin were used to activate and inhibit the AMPK pathway, respectively. Cell proliferation, apoptosis, differential gene expression and KEGG pathway analysis, intestinal paracellular permeability, intestinal morphology, and expression of HSP and TJ proteins, and p-AMPK were determined. L-Arginine promoted cell proliferation and re...
Source: Molecular Biology Reports - October 1, 2019 Category: Molecular Biology Authors: Xia Z, Huang L, Yin P, Liu F, Liu Y, Zhang Z, Lin J, Zou W, Li C Tags: Mol Biol Rep Source Type: research

Cloning, characterization, in vitro and in planta expression of a necrosis-inducing Phytophthora protein 1 gene npp1 from Phytophthora cinnamomi.
Abstract The soil-borne oomycete Phytophthora cinnamomi is a highly destructive Phytophthora species associated with the decline of forest. This pathogen secretes a novel class of necrosis-inducing proteins known as Nep1-like proteins (NLPs). In this work, we report the sequencing and molecular characterization of one of these proteins, more specifically the necrosis-inducing Phytophthora protein 1 (NPP1). The ORF of the npp1 gene (EMBL database AM403130) has 768 bp encoding a putative peptide of 256 amino acids with a molecular weight of approximately 25 kD. In order to understand its function...
Source: Molecular Biology Reports - September 30, 2019 Category: Molecular Biology Authors: Martins IM, Meirinho S, Costa R, Cravador A, Choupina A Tags: Mol Biol Rep Source Type: research

Evaluation of the CRISPR/Cas9 directed mutant TP53 gene repair ıng effect in human prostate cancer cell line PC-3.
In this study, we aimed to evaluate the CRISPR/Cas9 repairing efficiency on TP53 414delC (p.K139fs*31) null mutation, located in the TP53 gene, of human prostate cancer cell line PC-3 in combination with ssODNs. According to the next-generation sequencing results, TP53 414delC mutation was repaired with an efficiency of 19.95% and 26.0% at the TP53 414delC position with ssODN1 and ssODN2 accompanied by sgRNA2 guided CRISPR/Cas9, respectively. Besides, qPCR and immunofluorescence analysis showed that PC-3 cells, the TP53 414delC mutation of which were repaired, expressed wild type p53 again. Also, significantly increased nu...
Source: Molecular Biology Reports - September 30, 2019 Category: Molecular Biology Authors: Batır MB, Şahin E, Çam FS Tags: Mol Biol Rep Source Type: research

Glitazone loaded fat enhances adiponectin production and inhibits breast cancer cell proliferation.
This study investigates the potential of using adipose tissue laden with glitazones to act as a drug depot, increase adiponectin levels, and locally release glitazones to inhibit breast cancer cell proliferation. The breast cancer cell lines MCF-7 and MBA-MD-231, and the normal breast epithelial cell line MCF-10A were exposed to media containing a range of concentrations of recombinant adiponectin, pioglitazone, or conditioned media obtained from pioglitazone laden adipose tissue to determine the impact of the different treatments on cell proliferation. The MCF-7 cells demonstrated the greatest reduction in proliferation u...
Source: Molecular Biology Reports - September 30, 2019 Category: Molecular Biology Authors: Shea J, Terry C, Edwards K, Agarwal J Tags: Mol Biol Rep Source Type: research

IGS sequences in Cestrum present AT- and GC-rich conserved domains, with strong regulatory potential for 5S rDNA.
Abstract The 35S and 5S ribosomal DNA (rDNA) organized in thousands of copies in genomes, have been widely used in numerous comparative cytogenetic studies. Nevertheless, several questions related to the diversity and organization of regulatory motifs in 5S rDNA remain to be addressed. The 5S rDNA unit is composed of a conserved 120 bp length coding region and an intergenic spacer (IGS) containing potential regulatory motifs (Poly-T, AT-rich and GC-rich) differing in number, redundancy and position along the IGS. The Cestrum species (Solanaceae) have large genomes (about 10 pg/1C) and conserved 2n&thinsp...
Source: Molecular Biology Reports - September 30, 2019 Category: Molecular Biology Authors: de Souza TB, Gaeta ML, Martins C, Vanzela ALL Tags: Mol Biol Rep Source Type: research

Identification of a transforming growth factor- β type I receptor transcript in Eriocheir sinensis and its molting-related expression in muscle tissues.
This study investigates the roles of the TGF-β receptor in molting-related muscle growth in Eriocheir sinensis. Using the RT-PCR and RACE techniques, we obtained a 1722 bp cDNA sequence encoding a transforming growth factor-β type I receptor in Eriocheir sinensis, designated EsTGFBRI, which contains a 124 bp 5'-untranslated region, a 20 bp partial 3'-untranslated region and a 1578 bp open reading frame encoding 525 amino acids. The deduced EsTGFBRI contains an N-terminal 24 amino acid signal peptide, an activin type I and II receptor domain, a transmembrane helix region, a glycine-serine-rich ...
Source: Molecular Biology Reports - September 30, 2019 Category: Molecular Biology Authors: Tian Z, Peng H, Deng W, Jiao C Tags: Mol Biol Rep Source Type: research

Promoter activity of earthworm metallothionein in mouse embryonic fibroblasts.
r M Abstract The regulation of metallothionein (MT) gene expression as important part of the detoxification machinery is only scarcely known in invertebrates. In vertebrates, MT gene activation is mediated by the metal-transcription factor 1 (MTF-1) binding to metal response elements (MREs). In invertebrates, the mechanisms of MT gene activation seems to be more diverse. In some invertebrate species, MTF-1 orthologues as well as their ability to activate MT genes via MREs have been uncovered. Although earthworm MTs have been well studied, a MTF-1 orthologue has not yet been described and MT gene activation mechani...
Source: Molecular Biology Reports - September 30, 2019 Category: Molecular Biology Authors: Drechsel V, Fiechtner B, Höckner M Tags: Mol Biol Rep Source Type: research

Diagnostic accuracy of interferon (IFN)- γ inducible protein 10 (IP-10) as a biomarker for the discrimination of active and latent tuberculosis.
Diagnostic accuracy of interferon (IFN)-γ inducible protein 10 (IP-10) as a biomarker for the discrimination of active and latent tuberculosis. Mol Biol Rep. 2019 Sep 28;: Authors: Mamishi S, Mahmoudi S, Banar M, Hosseinpour Sadeghi R, Marjani M, Pourakbari B Abstract To assess the potency of Interferon (IFN)-γ inducible protein 10 (IP-10) stimulated by recombinant PE35 and PPE68 as a biomarker in differentiating between active and latent tuberculosis. Patients with active pulmonary TB (PTB) (n = 30), latent TB infection (LTBI) (n = 29), and BCG-vaccinated healt...
Source: Molecular Biology Reports - September 28, 2019 Category: Molecular Biology Authors: Mamishi S, Mahmoudi S, Banar M, Hosseinpour Sadeghi R, Marjani M, Pourakbari B Tags: Mol Biol Rep Source Type: research

Comparison of the novel dipstick DNA extraction technique with two established techniques for use in biological barcoding.
Abstract The novel dipstick DNA extraction method was tested for its reliability and usability for biological barcoding in comparison to a commercial kit and to a simplified isopropanol precipitation method using crayfish gill tissue. Following DNA extraction, the mitochondrial COI-gene was amplified in a PCR-reaction using a standard set of universal invertebrate primers. All three extraction techniques resulted in successful amplifications. With the dipstick method, PCR immediately follows the very brief DNA extraction technique. We suggest that the dipstick method is an affordable, efficient, and reliable DNA e...
Source: Molecular Biology Reports - September 26, 2019 Category: Molecular Biology Authors: Martello A, Lambert B, Johnston C, Cutler J, Stumpf CF Tags: Mol Biol Rep Source Type: research

The effects of transplanted mesenchymal stem cells treated with 17-b estradiol on experimental autoimmune encephalomyelitis.
Abstract The present study was conducted aimed at exploring the modulatory effects of 17-b estradiol (17-bED) on mesenchymal stem cells (MSCs) in the EAE (experimental autoimmune encephalomyelitis) animal model of multiple sclerosis (MS). Following the isolation of bone marrow-derived MSCs from the bilateral femurs and tibias of the male Wistar rats, the cells were harvested and cultured in the presence of 100 nM 17-bED for 24 h. EAE was induced in male Wistar rats (8-12 weeks old) using guinea pig spinal cord homogenate, in combination with the complete Freund's adjuvant. The MSC therapy was trigge...
Source: Molecular Biology Reports - September 25, 2019 Category: Molecular Biology Authors: Heidari Barchi Nezhad R, Asadi F, Abtahi Froushani SM, Hassanshahi G, Kaeidi A, Khanamani Falahati-Pour S, Hashemi Z, Mirzaei MR Tags: Mol Biol Rep Source Type: research

Transcriptome sequencing of Eospalax fontanierii to determine hypoxia regulation of cardiac fibrinogen.
Abstract With the increase in blood viscosity, the blood circulation resistance will increase when animals are in hypoxia. However, these phenomenons do not appear in hypoxic-adapted animals. Eospalax fontanierii is a subterranean rodent and is an ideal species for research in hypoxia adaptation. Eighteen healthy adult E. fontanierii individuals were equally divided into three groups that were exposed to 21% O2 for 1 week, 10.5% O2 for 44 h, and 6.5% O2 for 6 h, and then, the hearts were collected for transcriptome sequencing. After differentially expressed analysis, fibrinogen genes were selected f...
Source: Molecular Biology Reports - September 24, 2019 Category: Molecular Biology Authors: Xu L, Hao Z, Lin J, Zhao Q, Zhang B, Li G, He J, Li J Tags: Mol Biol Rep Source Type: research

Isolation and characterization of eleven novel microsatellite markers for fine-scale population genetic analyses of Gammarus pulex (Crustacea: Amphipoda).
ki SG Abstract The freshwater amphipod species Gammarus pulex (Linnaeus, 1758) is widespread across Europe and Asia and is able to live in a broad range of environmental conditions. Yet, it is of great interest to which degree it is able to tolerate and adapt to the current rapid anthropogenic environmental changes affecting its habitat, such as pollution, changes in river morphology, and invasions of alien gammarid species. Microsatellite primers for genetic population studies with G. pulex have been developed but due to the existence of several genetically different lineages within the species, the application o...
Source: Molecular Biology Reports - September 24, 2019 Category: Molecular Biology Authors: Švara V, Norf H, Luckenbach T, Brack W, Michalski SG Tags: Mol Biol Rep Source Type: research

Dysregulation of non-histone molecule miR205 and LRG1 post-transcriptional de-regulation by SETD1A in triple negative breast cancer.
This study aims to understand SETD1A mechanisms in TNBC development in two TNBC cell lines. SETD1A was transiently transfected in MDA-MB-468 (FEC good prognosis) and Hs578T (FEC poor prognosis). Regulation of potential targets miR205, EMT marker ZEB1 and LRG1 and proliferative marker Ki-67 were tested by RqPCR to elucidate SETD1A interactions. This study displayed significant recovery of miR205 with SETD1A depletion and reduction of ZEB1 in MDA-MB-468. However, ZEB1 remained unchanged in Hs578T indicating ZEB1 regulation may be outcompeted by other mechanisms associated with aggressive cell line characteristics and the exp...
Source: Molecular Biology Reports - September 24, 2019 Category: Molecular Biology Authors: Mohamad Hanif EA Tags: Mol Biol Rep Source Type: research

Transposable element discovery and characterization of LTR-retrotransposon evolutionary lineages in the tropical fruit species Passiflora edulis.
Abstract A significant proportion of plant genomes is consists of transposable elements (TEs), especially LTR retrotransposons (LTR-RTs) which are known to drive genome evolution. However, not much information is available on the structure and evolutionary role of TEs in the Passifloraceae family (Malpighiales order). Against this backdrop, we identified, characterized, and inferred the potential genomic impact of the TE repertoire found in the available genomic resources for Passiflora edulis, a tropical fruit species. A total of 250 different TE sequences were identified (96% Class I, and 4% Class II), correspon...
Source: Molecular Biology Reports - September 24, 2019 Category: Molecular Biology Authors: da Costa ZP, Cauz-Santos LA, Ragagnin GT, Van Sluys MA, Dornelas MC, Berges H, de Mello Varani A, Vieira MLC Tags: Mol Biol Rep Source Type: research

Recombinase polymerase amplification-nucleic acid lateral flow immunoassays for Newcastle disease virus and infectious bronchitis virus detection.
The objective of our study is developing a simple, rapid and field applicable recombinase polymerase amplification (RPA)-nucleic acid lateral flow (NALF) immunoassay for detection of NDV and IBV. Isothermal amplification of the matrix protein (M) gene of NDV and the nucleoprotein (N) gene of IBV was implemented via recombinase polymerase amplification at 38 °C for 40 min and 20 min, respectively using modified labeled primers. NALF device used in this study utilizes antibodies for detection of labeled RPA amplicons. The results revealed that RPA-NALF immunoassays can detect both viruses after 40 min...
Source: Molecular Biology Reports - September 23, 2019 Category: Molecular Biology Authors: El-Tholoth M, Branavan M, Naveenathayalan A, Balachandran W Tags: Mol Biol Rep Source Type: research

Aberrant expression of long noncoding RNAs in the serum and myocardium of spontaneous hypertensive rats.
Abstract Circulating long noncoding RNAs as biomarkers of diseases have attracted increasing attention recently. However, circulating lncRNAs in hypertension is still unexplored niche. The levels of lncRNAs GAS5, NR024118, MRAK134679, AX765700 and MRNR026574 were measured in the serum and myocardium of hypertensive rats and normal controls with real time PCR. The levels of GAS5 were significantly higher both in the myocardium (P = 0.0067) and serum (P 
Source: Molecular Biology Reports - September 23, 2019 Category: Molecular Biology Authors: Wu Y, Zhang Z, Ren S, Li K, Ning Q, Jiang X Tags: Mol Biol Rep Source Type: research

Myocardial regeneration: role of epicardium and implicated genes.
Abstract Lower invertebrates' hearts such as those of zebrafish have the capacity for scarless myocardial regeneration which is lost by mammalian hearts as they form a fibrotic scar tissue instead of regenerating the injured area. However, neonatal mammalian hearts have a remarkable capacity for regeneration highlighting conserved evolutionary mechanisms underlying such a process. Studies investigated the underlying mechanism of myocardial regeneration in species capable to do so, to see its applicability on mammals. The epicardium, the mesothelial outer layer of the vertebrate heart, has proven to play an importa...
Source: Molecular Biology Reports - September 23, 2019 Category: Molecular Biology Authors: Saifi O, Ghandour B, Jaalouk D, Refaat M, Mahfouz R Tags: Mol Biol Rep Source Type: research

The effects of serum levels, and alterations in the genes of binding protein and receptor of vitamin D on gastric cancer.
il;ükhüseyin Ö Abstract Due to many biological cell functions of vitamin D including regulation of cell survival, proliferation and differentiation, the metabolism of itself gains importance in the development of several types of cancer. This case-control study was designed to evaluate the risk of gastric cancer development in terms of VDR rs2228570 & rs731236, and VDBP rs7041 polymorphisms, and serum levels of vitamin D. The study consists of 77 gastric cancer patients and 84 healthy individuals. VDR and VDBP gene polymorphisms and vitamin D levels were determined by using PCR-RFLP and HPLC met...
Source: Molecular Biology Reports - September 23, 2019 Category: Molecular Biology Authors: Durak Ş, Gheybi A, Demirkol Ş, Arıkan S, Zeybek ŞÜ, Akyüz F, Yaylım İ, Küçükhüseyin Ö Tags: Mol Biol Rep Source Type: research

Effect of single nucleotide polymorphisms in SEPS1 and SEPP1 on expression in the protein level in metabolic syndrome in subjects with cardiovascular disease.
This study aims to investigate the association between the risk of MetS and four polymorphisms SEPS1 (rs28665122, rs4965373), SEPP1 (rs7579, rs3877899) in an Iranian population. The sample of this case-control study consisted of 132 Iranian patients with cardiovascular disease (71 MetS and 65 non-MetS subjects) from December 2015 to March 2016. Demographic data, medical history, and para-clinical were measured, and Taqman probes were used for allelic discrimination. The level of the SelS and the SePP were measured by the ELIZA method. No significant differences were found in the genotype frequencies of SEPS1 (rs4...
Source: Molecular Biology Reports - September 21, 2019 Category: Molecular Biology Authors: Gharipour M, Ouguerram K, Nazih EH, Salehi M, Behmanesh M, Razavi R, Gharipour A, Diantkhah M, Sadeghi M Tags: Mol Biol Rep Source Type: research

The impaired gene expression of adenosine monophosphate-activated kinase (AMPK), a key metabolic enzyme in leukocytes of newly diagnosed rheumatoid arthritis patients.
This study aims to evaluate the alteration of AMPK gene expression in peripheral blood leukocytes of RA patients and its effects on disease severity as well as plasma levels of anti-inflammatory cytokines. 60 RA patients, including 30 newly diagnosed and 30 patients whose disease were under controlled with the combinational disease-modifying anti-rheumatic drug (DMARD), as well as 30 healthy subjects, were enrolled in our study. The gene expression of AMPK was evaluated using real-time PCR method. The plasma concentrations of IL-10 and TGF-β1 were measured using sandwich ELISA. The gene expression of AMPK was signific...
Source: Molecular Biology Reports - September 20, 2019 Category: Molecular Biology Authors: Samimi Z, Kardideh B, Zafari P, Bahrehmand F, Roghani SA, Taghadosi M Tags: Mol Biol Rep Source Type: research