Hypericum perforatum L.: a medicinal plant with potential as a curative agent against obesity-associated complications.
In this study, 3T3-L1 adipocytes were used to mimic the adipocyte differentiation associated with obesity in cellular terms. Lipoprotein lipase (Lpl), Diacylglycerol-O-acyltransferase 1 (Dgat1), Fatty acid synthase (Fasn) markers were used to study the lipid accumulation, and Collagen V (ColV) was used to study cell elasticity to investigate the relationship of the effects of the administration of Hypericum perforatum L. with obesity. PMID: 33090307 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 22, 2020 Category: Molecular Biology Authors: Tokgöz HB, Altan F Tags: Mol Biol Rep Source Type: research

Identification of an individual with a SYGNAP1 pathogenic mutation in India.
In this report, we discuss the finding of a novel mutation in one of the genes, SYNGAP1, implicated in ASD/ID. Besides, we discuss the current treatment prescribed to the patient and the progress of global developmental of the child. PMID: 33090308 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 22, 2020 Category: Molecular Biology Authors: Verma V, Mandora A, Botre A, Clement JP Tags: Mol Biol Rep Source Type: research

Rosuvastatin inhibit spheroid formation and epithelial-mesenchymal transition (EMT) in prostate cancer PC-3 cell line.
Abstract There is a growing body of evidence suggesting antitumor activity of statins. In metastasis and invasion of cancer the Epithelial-Mesenchymal Transition (EMT) of cancerous cells is an important process. Our goal was to understand the effect of Rosuvastatin on the EMT process in human prostate cancer cell line PC-3 cells in adherent 2 dimensional (2D) and spheroid 3 dimensional (3D) culture. PC-3 cells were cultured in adherence and/or spheroid culture system. The cells were treated with different concentrations of Rosuvastatin. After 96 h, the cell proliferation, viability, type and number of spheroi...
Source: Molecular Biology Reports - October 21, 2020 Category: Molecular Biology Authors: Deezagi A, Safari N Tags: Mol Biol Rep Source Type: research

Cognitive changes in patient living with HIV-AIDS and apolipoprotein-E polymorphism: is there an association?
This study aimed to evaluate the association between APOE polymorphisms and cognitive disorders in patients with HIV-AIDS. This was a cross-sectional study comprising 133 patients aged 19-59 years old, with HIV-AIDS and were assisted at the infectious disease outpatient clinics at Hospital Universitário Oswaldo Cruz, in Recife, Brazil. For cognitive evaluation, Mini-Mental State Examination test (MMSE) and Montreal Cognitive Assessment test (MoCA) were used. The determination of APOE gene polymorphism was performed by using the PCR-RFLP technique. Sociodemographic and clinical characteristics were not...
Source: Molecular Biology Reports - October 21, 2020 Category: Molecular Biology Authors: Brito-Marques PR, Rocha-Filho PAS, Dellalibera E, Muniz MTC, Cunha-Correia C Tags: Mol Biol Rep Source Type: research

COS-7 cells are a cellular model to monitor polyomavirus JC miR-J1-5p expression.
Abstract Polyomavirus JC (JCPyV) is a ubiquitous human neurotropic virus that can cause progressive multifocal leukoencephalopathy (PML), sometimes as a consequence of drug treatment for disabling diseases, including Multiple Sclerosis. JCPyV expresses microRNAs (miRNAs), and in particular miR-J1-5p, but at now we have limited knowledge regarding this aspect. In the present study the expression of JCPyV miR-J1-5p was measured in infected COS-7, to verify if and when this miRNA is expressed in a cell model of JCPyV-MAD-4 strain infection. Results showed that miR-J1-5p expression was relatively constant inside ...
Source: Molecular Biology Reports - October 21, 2020 Category: Molecular Biology Authors: Agostini S, Mancuso R, Costa AS, Guerini FR, Clerici M Tags: Mol Biol Rep Source Type: research

Genetic alterations and clinical dimensions of oral cancer: a review.
Abstract Oral cancer ranks sixth most prevalent type of cancer worldwide due to its alarming increase every year. Progression of oral cancer depends on various heterogeneity pathways, but their exact mechanism remains unclear. Genetic aberrations on oral cancer cells set back the effectiveness of existing therapies and make it more challenging by triggering drug resistance. To understand the intricate details of oral cancer pathogenesis and for advancing current therapies, genetic modifications are the most promising approach. In this review, we tabulated the information on genetic alterations, microbial asso...
Source: Molecular Biology Reports - October 21, 2020 Category: Molecular Biology Authors: Karunakaran K, Muniyan R Tags: Mol Biol Rep Source Type: research

Uncovering epigenetic landscape: a new path for biomarkers identification and drug development.
te; R Abstract Scientific advances in recent decades have revealed an incredible degree of plasticity in gene expression in response to various environmental, nutritional, physiological, pathological, and behavioral conditions. Epigenetics emerges in this sense, as the link between the internal (genetic) and external (environmental) factors underlying the expression of the phenotype. Methylation of DNA and histone post-translationa modifications are canonical epigenetic events. Additionally, noncoding RNAs molecules (microRNAs and lncRNAs) have also been proposed as another layer of epigenetic regulation. Together...
Source: Molecular Biology Reports - October 21, 2020 Category: Molecular Biology Authors: de Oliveira DT, Guerra-Sá R Tags: Mol Biol Rep Source Type: research

Mitochondrial DNA markers reveal genetic connectivity among populations of Osteoglossiform fish Chitala chitala.
Abstract Genetic diversity and population structure in Indian featherback fish, Chitala chitala (Hamilton, 1822) was investigated by combined analyses of two full mitochondrial genes, ATPase 6/8 and Cytochrome b. A total of 403 individuals, collected from 14 rivers yielded 61 haplotypes. Hierarchical partitioning analysis identified 19.01% variance 'among' and 80.99% variance 'within groups and populations'. The mean coefficient of genetic differentiation (FST) was observed to be significant 0.26 (p 
Source: Molecular Biology Reports - October 20, 2020 Category: Molecular Biology Authors: Dutta N, Singh RK, Pathak A, Mohindra V, Mandal S, Kaur G, Lal KK Tags: Mol Biol Rep Source Type: research

Follicle inhibition at the primordial stage without increasing apoptosis, with a combination of everolimus, verapamil.
Abstract The aim of the present study was to test whether inhibition of ovarian primordial follicles and subsequent activation can be achieved by transient mTOR inhibition. In this preclinical investigation, forty-five female immature Wistar rats were randomized in 5 groups. The control group received subcutaneous saline injections. The other groups received Everolimus, Everolimus plus Verapamil, Everolimus plus Fisetin, and Fisetin alone. Primary and secondary outcomes were measured in the left ovary after a treatment period of 8 weeks. Ten days later, animals received 35 IU FSH for 4 days and 35&n...
Source: Molecular Biology Reports - October 20, 2020 Category: Molecular Biology Authors: Pargianas M, Kosmas I, Papageorgiou K, Kitsou C, Papoudou-Bai A, Batistatou A, Markoula S, Salta S, Dalkalitsis A, Kolibianakis S, Tarlatzis BC, Georgiou I, Michaelidis TM Tags: Mol Biol Rep Source Type: research

Berberine reverses epithelial-mesenchymal transition and modulates histone methylation in osteosarcoma cells.
In conclusion, our findings suggest that berberine inhibits proliferation and migration of osteosarcoma cells and most importantly reverses EMT along with modulation of key epigenetic regulators. PMID: 33074411 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 19, 2020 Category: Molecular Biology Authors: Mishra R, Nathani S, Varshney R, Sircar D, Roy P Tags: Mol Biol Rep Source Type: research

CRISPR based targeted genome editing of Chlamydomonas reinhardtii using programmed Cas9-gRNA ribonucleoprotein.
In this study, we have demonstrated targeted gene knock-out in C. reinhardtii using programmed RNPs. Targeted editing of MAA7 gene was confirmed by sequencing the clones that were resistant to 5-Fluoroindole (5-FI). Non-homologous end joining (NHEJ) repair mechanism led to insertion, deletion, and/or base substitution in the Cas9 cleavage vicinity, encoding non-functional MAA7 protein product (knock-out), conferring resistance to 5-FI. Here, we report an efficient protocol for developing knock-out mutants in Chlamydomonas using CRISPR-Cas9 RNPs. The high potential efficiency of editing may also eliminate the need to select...
Source: Molecular Biology Reports - October 19, 2020 Category: Molecular Biology Authors: Dhokane D, Bhadra B, Dasgupta S Tags: Mol Biol Rep Source Type: research

Pro-inflammatory cytokine response pre-dominates immuno-genetic pathway in development of rheumatoid arthritis.
Abstract Rheumatoid arthritis (RA) is a crucial inflammatory joint disease characterized by loss of self-tolerance and severe cartilage loss, autoimmune, and subchondral bone erosions. Cytokines are the key regulators of inflammatory responses. Homeostatic imbalances in pro- and anti-inflammatory cytokine activities can result in pathogenic inflammatory reactions. These imbalances could be initiated by environmental factors but the ability to define the threshold of environmental impact relies on the genetic background of the pro- and anti-inflammatory cytokines. To address this a case-control association study wa...
Source: Molecular Biology Reports - October 19, 2020 Category: Molecular Biology Authors: Krishna Priya EK, Srinivas L, Rajesh S, Sasikala K, Banerjee M Tags: Mol Biol Rep Source Type: research

Molecular and morphometric divergence of four species of butterflies (Nymphalidae and Pieridae) from the Western Himalaya, India.
Abstract Morphometric and molecular divergence among four butterfly species of the families Nymphalidae and Pieridae from the western Himalaya region were investigated using molecular tools, traditional morphometric measures and a truss network system. The considered species were Danaus chrysippus, Vanessa cardui, Pieris brassicae and Pieris canidia. Traditional taxonomy is sometimes unable to discriminate cryptic species or species that have close morphological features. Although taxonomists carefully examine external body features to differentiate the species; however, there is a risk for misidentification durin...
Source: Molecular Biology Reports - October 18, 2020 Category: Molecular Biology Authors: Singh VK, Joshi PC, Gupta SK Tags: Mol Biol Rep Source Type: research

The role of AMPK in metabolism and its influence on DNA damage repair.
Abstract One of the most complex health disproportions in the human body is the metabolic syndrome (MetS). It can result in serious health consequences such as type 2 diabetes mellitus, atherosclerosis or insulin resistance. The center of energy regulation in human is AMP-activated protein kinase (AMPK), which modulates cells' metabolic pathways and protects them against negative effects of metabolic stress, e.g. reactive oxygen species. Moreover, recent studies show the relationship between the AMPK activity and the regulation of DNA damage repair such as base excision repair (BER) system, which is presented in r...
Source: Molecular Biology Reports - October 18, 2020 Category: Molecular Biology Authors: Szewczuk M, Boguszewska K, Kaźmierczak-Barańska J, Karwowski BT Tags: Mol Biol Rep Source Type: research

Impact of nano-selenium on nuclear maturation and genes expression profile of buffalo oocytes matured in vitro.
Abstract Supplementation of maturation media with antioxidant (bulk form) improves oocyte maturation. However, the influence of adding antioxidant (nano-particles) on oocyte maturation is not well known. We aimed to evaluate the effect of selenium nano-particles (SeNP) and bulk selenium (Se) on buffalo oocytes maturation, in terms nuclear maturation and molecular level. Oocytes were distributed into four groups; 1st group was control, 2nd group was supplied with Se (10 ng/ml), 3rd and 4th groups were supplied with 1 µg/ml SeNP (67 nm), and SeNP (40 nm), respectively. Matured oocytes were ...
Source: Molecular Biology Reports - October 17, 2020 Category: Molecular Biology Authors: El-Naby AAH, Ibrahim S, Hozyen HF, Sosa ASA, Mahmoud KGM, Farghali AA Tags: Mol Biol Rep Source Type: research

The effect of blastomere loss during frozen embryo transfer on the transcriptome of offspring's umbilical cord blood.
In conclusions, our pilot study sheds light on the mechanism underlying how human blastomere loss may affect offspring at the gene expression level. These conclusions are, however, only suggestive, as the current study is based on a very limited sample size and type or nature of biological samples. Additional studies with larger sample sizes and independent experiments with placental samples should be conducted to verify these findings. PMID: 33068229 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 17, 2020 Category: Molecular Biology Authors: Wu YT, Dong ZH, Li C, Zhou DZ, Zhang JY, Wu Y, Xu JJ, Wang Y, Ye XQ, Sheng JZ, Wang L, Huang HF Tags: Mol Biol Rep Source Type: research

Phytopathogenic oomycetes: a review focusing on Phytophthora cinnamomi and biotechnological approaches.
ina A Abstract The Phytophthora genus is composed, mainly, of plant pathogens. This genus belongs to the Oomycete class, also known as "pseudo-fungi", within the Chromista Kingdom. Phytophthora spp. is highlighted due to the significant plant diseases that they cause, which represents some of the most economically and cultural losses, such as European chestnut ink disease, which is caused by P. cinnamomi. Currently, there have been four genome assemblies placed at the National Center for Biotechnology Information (NCBI), although the progress to understand and elucidate the pathogenic process of P. cinna...
Source: Molecular Biology Reports - October 17, 2020 Category: Molecular Biology Authors: de Andrade Lourenço D, Branco I, Choupina A Tags: Mol Biol Rep Source Type: research

Identification and characterization of multiple abiotic stress tolerance genes in wheat.
Abstract Wheat is produced worldwide over six continents with the European Union, China, India, Russia, and the United States as major producer countries. The productivity was recorded 749 million tons by harvesting from 220-million-hectare land. It is the need of the hour to develop stress-tolerant wheat varieties to enhance the productivity by 60% to provide food security to 9.6 billion-world population by 2050. Although the genotypes have been identified for heat, drought and salt tolerance, their underlying mechanism for tolerance is poorly understood. The detailed understanding of the mechanism and identifica...
Source: Molecular Biology Reports - October 17, 2020 Category: Molecular Biology Authors: Kumar R, Masthigowda MH, Kaur A, Bhusal N, Pandey A, Kumar S, Mishra C, Singh G, Singh GP Tags: Mol Biol Rep Source Type: research

Deficiency of C1q/TNF-related protein 3 (CTRP3) decreases adipose tissue weight in diet-induced obesity mice.
Abstract Adipokines are important regulators of lipid and glucose metabolism. A family of adiponectin paralogs is known as C1q and tumor necrosis factor (TNF)-related proteins (CTRPs). One line of Ctrp3-deficient mice shows reduced liver size in response to obesity. We generated and characterized another line of Ctrp3 knockout (KO) mice to reveal novel physiological functions of CTRP3. Interestingly, high fat diet (HFD)-fed Ctrp3 KO mice displayed a decrease in the epididymal white adipose tissue (WAT) weight to total body weight ratio. Histologically, adipocyte size was significantly smaller in the epididyma...
Source: Molecular Biology Reports - October 15, 2020 Category: Molecular Biology Authors: Maeda T, Wakisaka S Tags: Mol Biol Rep Source Type: research

Chromosomal instability reducing effect of paclitaxel and lapatinib in mouse embryonic stem cells with chromosomal abnormality.
Abstract Chromosomal abnormalities, as a frequent phenomenon in cultured embryonic stem cells (ESCs), is a major obstacle in the ESC application in regenerative medicine. Recent studies showed that aneuploid embryonic stem cells of humans and mice are more vulnerable to anticancer drugs, compared with normal cells. The aim of the current study was to evaluate effects of three anticancer drugs, paclitaxel, lapatinib and bortezomib, on mouse embryonic stem cells (mESCs) as a suitable and available model. To assess in vitro cell toxicity, two mESC lines were treated with the aforementioned drugs. Using G-band karyoty...
Source: Molecular Biology Reports - October 15, 2020 Category: Molecular Biology Authors: Mirzaei-Seresht B, Bazrgar M, Sheidai M, Hassani SN, Masoudi NS, Mollammohammadi S Tags: Mol Biol Rep Source Type: research

The incidence of NOS3 gene polymorphisms on newborns with large and small birth weight.
In conclusion, the NOS3 polymorphisms do not appear to be a factor to inadequate birth weight. However, the G894T and VNTR 4b/a polymorphisms, and the haplotype 4aTC, seem to influence the occurrence of SGA. PMID: 33063148 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 15, 2020 Category: Molecular Biology Authors: de Aragão Santos TW, Dos Santos Catena A, da Silva Mattos S, de Lima Filho JL, Gondim Martins DB Tags: Mol Biol Rep Source Type: research

Genetic characterization and population structure of different coat colour variants of Badri cattle.
Abstract The present study aimed to genetically characterize the Badri cattle and its three colour variants and assess their population structure using 24 microsatellite markers. Out of 96 animals analyzed, 32 each were collected from grey (GVBC), brown (BrVBC) and black (BVBC) colour variants of Badri cattle (BC). The genetic diversity parameters including allele frequencies, observed and effective number of alleles, observed and expected heterozygosity, PIC, Shannon's indices and F-statistics were estimated using POPGENE software. Bottleneck analysis was performed using both qualitative and quantitative approach...
Source: Molecular Biology Reports - October 15, 2020 Category: Molecular Biology Authors: Dar AH, Kumar S, Mukesh M, Ahmad SF, Singh DV, Sharma RK, Ghosh AK, Singh B, Rahman JU, Sodhi M Tags: Mol Biol Rep Source Type: research

Phylogenetic analysis of two new complete genomes of the hepatitis E virus (HEV) genotype 3 from Thailand.
Abstract Hepatitis E virus (HEV) is a causative agent of acute viral hepatitis globally. Evolutionary phylogeny classifies the HEV into eight genotypes that correlate with the viral transmission. Only four genotypes have been proven to be responsible for transmission in humans. However, there has been no report on the genomics and genotyping of HEV in Thailand during the past ten years. Here, we identified the genotype distributions of the Thai isolates of HEV and we sequenced two HEV genomes. We screened for 18 Thai isolates of HEV from Siriraj Hospital in Bangkok, from 2014-2016. The HEV genomes were sequenced f...
Source: Molecular Biology Reports - October 14, 2020 Category: Molecular Biology Authors: Chanmanee T, Ajawatanawong P, Louisirirotchanakul S, Chotiyaputta W, Chainuvati S, Wongprompitak P Tags: Mol Biol Rep Source Type: research

Molecular and hormonal changes caused by long-term use of high dose pregabalin on testicular tissue: the role of p38 MAPK, oxidative stress and apoptosis.
Abstract In 1990, pregabalin was introduced as a novel antiepileptic drug that acts by binding selectively to the alpha-2-delta subunits of voltage-gated calcium channels resulting in increasing neuronal GABA levels and inhibiting the release of exciting neurotransmitters. The aim of our study is to assess the hazardous effects of prolonged high-dose pregabalin (like that abused by addicts) on testes and to clarify the potential causative mechanisms. The current study was conducted on 70 adult male Wistar albino rats which were divided into 7 groups. In our study we evaluated the effect of pregabalin, at concentra...
Source: Molecular Biology Reports - October 13, 2020 Category: Molecular Biology Authors: Taha SHN, Zaghloul HS, Ali AAER, Rashed LA, Sabry RM, Gaballah IF Tags: Mol Biol Rep Source Type: research

MicroRNA-218 competes with differentiation media in the induction of osteogenic differentiation of mesenchymal stem cell by regulating β-catenin inhibitors.
This study provided evidence that miR-218 can promote osteogenic differentiation of AD-MSCs even in conditional media. Therefore, our findings suggest miR-218 as a putative novel therapeutic candidate in the context of osteoporosis and other bone metabolism-related diseases. PMID: 33051753 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 13, 2020 Category: Molecular Biology Authors: Karimi Z, Seyedjafari E, Khojasteh A, Hashemi SM, Kazemi B, Mohammadi-Yeganeh S Tags: Mol Biol Rep Source Type: research

Increased phenolic acid and tanshinone production and transcriptional responses of biosynthetic genes in hairy root cultures of Salvia przewalskii Maxim. treated with methyl jasmonate and salicylic acid.
This study provides, for the first time, an efficient tanshinone and phenolic acid production method for S. przewalskii. PMID: 33048323 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 13, 2020 Category: Molecular Biology Authors: Li J, Li B, Luo L, Cao F, Yang B, Gao J, Yan Y, Zhang G, Peng L, Hu B Tags: Mol Biol Rep Source Type: research

Progression of heart failure is attenuated by antioxidant therapy with N-acetylcysteine in myocardial infarcted female rats.
This study investigated the therapeutic potential of N-acetylcysteine (NAC) in the treatment of heart failure in female rats. Myocardial infarcted (MI) rats were given NAC (250 mg/kg/day p.o.) during 28 days after surgery (MI + NAC) or vehicle (MI + Placebo), and sham-operated rats received the same treatments (Sham + NAC and Sham + Placebo). Electrocardiographic and echocardiographic analyses were performed in the last week of treatment. Cardiac mRNA levels of types I and II superoxide dismutase (SOD), catalase, types I and III glutathione peroxidase (GPX), nerve growth factor (NGF), β1-adrenergic receptor (β1AD...
Source: Molecular Biology Reports - October 13, 2020 Category: Molecular Biology Authors: Costa CRM, Seara FAC, Peixoto MS, Ramos IP, Barbosa RAQ, Carvalho AB, Fortunato RS, Silveira ALB, Olivares EL Tags: Mol Biol Rep Source Type: research

Dexmedetomidine alleviates sevoflurane-induced neurotoxicity via mitophagy signaling.
Abstract Dexmedetomidine, a class of α2-adrenergic agonist, was reported to exert a neuroprotective effect on sevoflurane-induced neurotoxicity. However, the specific mechanisms have not been fully clarified yet. The aim of our study is to uncover the role of dexmedetomidine in sevoflurane-induced neurotoxicity. The rats pretreated with dexmedetomidine and/or Rapamycin 3-Methyladenine were housed in a box containing 30% O2, 68% N2 and 2% sevoflurane for 4 h for anesthesia. 24 h after drug injection, Morris water maze test was used to evaluate rats' learning and memory ability. Hematoxylin & eosin (H...
Source: Molecular Biology Reports - October 12, 2020 Category: Molecular Biology Authors: Suo L, Wang M Tags: Mol Biol Rep Source Type: research

Honey protects against chronic unpredictable mild stress induced- intestinal barrier disintegration and hepatic inflammation.
Abstract Chronic stress is linked to liver injury by increasing intestinal permeability to lipopolysaccharide (LPS), which in turn can result in systemic and liver inflammation and damage. Beneficial effect of honey in the prevention of liver injury has been shown in previous studies, but mechanisms underlying are still less known. Here, we examined the therapeutic impacts of honey on intestinal nuclear factor-κB (NF-κB; an important regulator of stress-induced immune and inflammatory responses) and ileal tight junction (TJ) proteins of claudin-1 and ZO-1, serum LPS, liver inflammation and oxidative ma...
Source: Molecular Biology Reports - October 12, 2020 Category: Molecular Biology Authors: Mehranfard N, Yazdi A, Rafiei A, Shakerin Z, Ghasemi M Tags: Mol Biol Rep Source Type: research

The clinical significance of circulating DSCAM-AS1 in patients with ER-positive breast cancer and construction of its competitive endogenous RNA network.
Abstract Long Non-Coding RNAs (lncRNAs), with diagnostic and therapeutic applications in malignancies, are newly described tumour-related molecules. Here, we reported the importance of circulating DSCAM-AS1 as the biomarker to detect Estrogen Receptor (ER)-positive breast cancer (BC) cases. Moreover, the expression of a BC-associated lncRNAs, namely DSCAM-AS1, was measured in tumoural and Paired Adjacent Non-Tumoral (PANT) tissue, as well as plasma, using Real-Time Polymerase Chain Reaction (RT-PCR). Besides, the correlations between gene expression and the clinicopathological features were analyzed. The diagnosti...
Source: Molecular Biology Reports - October 11, 2020 Category: Molecular Biology Authors: Moradi MT, Fallahi H, Rahimi Z Tags: Mol Biol Rep Source Type: research

Fingerprint identification of white clover cultivars based on SSR molecular markers.
Abstract White clover (Trifolium repens L.) is an important perennial legume forage with high productivity and quality. To strengthen the basic research on the genetic characteristics, fingerprint identification and adaptability of white clover germplasm resources, Simple sequence repeat (SSR) molecular markers were applied to 10 white clover cultivars to assess the genetic diversity and related lines of white clover at the molecular level in order to lay a theoretical foundation for the selection of high-quality seeds and cultivars of white clover. A total of 120 different bands were amplified by 29 pairs of SSR ...
Source: Molecular Biology Reports - October 10, 2020 Category: Molecular Biology Authors: Ma S, Han C, Zhou J, Hu R, Jiang X, Wu F, Tian K, Nie G, Zhang X Tags: Mol Biol Rep Source Type: research

Quercetin, chrysin, caffeic acid and ferulic acid ameliorate cyclophosphamide-induced toxicities in SH-SY5Y cells.
In this study protective effects of quercetin, chrysin, caffeic acid and ferulic acid against CP-induced cell toxicity in SH-SY5Y cells was evaluated by cell proliferation assay, lipid peroxidation (LPO) analysis to decipher antioxidant capacity, tunel assay and qRT-PCR experiments to examine anti-apoptotic activities of the antioxidants. The results showed that CP-induced cell toxicity in SH-SY5Y cells and treatments with the antioxidants suppressed the cell death. Our results suggests that these anti-oxidants protected SH-SY5Y cells via a decrease in LPO levels, downregulating the expression of Cas-3, Cyt c and Bax and u...
Source: Molecular Biology Reports - October 10, 2020 Category: Molecular Biology Authors: Ayna A, Özbolat SN, Darendelioglu E Tags: Mol Biol Rep Source Type: research

Prospects of next generation sequencing in lentil breeding.
Abstract Lentil is an important food legume crop that has large and complex genome. During past years, considerable attention has been given on the use of next generation sequencing for enriching the genomic resources including identification of SSR and SNP markers, development of unigenes, transcripts, and identification of candidate genes for biotic and abiotic stresses, analysis of genetic diversity and identification of genes/ QTLs for agronomically important traits. However, in other crops including pulses, next generation sequencing has revolutionized the genomic research and helped in genomic assisted breed...
Source: Molecular Biology Reports - October 10, 2020 Category: Molecular Biology Authors: Kumar J, Sen Gupta D Tags: Mol Biol Rep Source Type: research

Mediation of induced systemic resistance by the plant growth-promoting rhizobacteria Bacillus pumilus S2-3-2.
Abstract Plant-rhizobacteria interaction and co-evolution developed adaptive strategies which may help the plant survive in nature. Plant rhizosphere soil isolates were analyzed to investigated the effects of rhizobacteria for promoting plant growth and suppress plant disease. Bacterial strains which isolated from plant rhizosphere soil were screened for elicitation of induced systemic resistance (ISR) on tobacco. Strain S2-3-2 results in significant reduction of disease severity on tobacco, it was identified as Bacillus pumilus by multilocus sequence analysis (MLSA). Strain S2-3-2 was deeper studied for pepp...
Source: Molecular Biology Reports - October 10, 2020 Category: Molecular Biology Authors: Li W, Lee SY, Cho YJ, Ghim SY, Jung HY Tags: Mol Biol Rep Source Type: research

Sulforaphane modulates CX3CL1/CX3CR1 axis and inflammation in palmitic acid-induced cell injury in C2C12 skeletal muscle cells.
Abstract Studies have shown that sulforaphane (SFN) has potent anti-inflammatory and free radical scavenging effects on obesity and associated disorder such as diabetes, polycystic ovary syndrome, and metabolic syndrome. fractalkine (CX3CL1) and its receptor, CX3CR1, play an important role in muscle metabolism by improving insulin-sensitizing effects. Here, in this study we examined the SFN effect on CX3CL1 and its receptor, CX3CR1, in C2C12 myotubes in palmitic acid (PA)-induced oxidative stress and inflammation. The results showed that PA (750 μM) evoked lipotoxicity as a reduction in cell viability, inc...
Source: Molecular Biology Reports - October 9, 2020 Category: Molecular Biology Authors: Faridvand Y, Haddadi P, Nejabati HR, Ghaffari S, Zamani-Gharehchamani E, Nozari S, Nouri M, Jodati A Tags: Mol Biol Rep Source Type: research

Evaluation of the genetic diversity of wild Salvadora persica 'Arak' from Saudi Arabia.
Abstract Assessment of genetic diversity is crucial for efficient selection genotypes in plant breeding and improvement programs. Studies of genetic diversity of S. persica are rare relative to the large species diversity in Saudi Arabia, despite its valuable importance as one of the most popular medicinal plants. We investigate the genetic variability and genetic differentiation among and within wild Salvadora persica populations distributed in four regions of Saudi Arabia. Twelve sequence-related amplified polymorphism (SRAP) primers combination generated 326 alleles, with an average of 27.2 alleles pe...
Source: Molecular Biology Reports - October 9, 2020 Category: Molecular Biology Authors: ALmohisen IA, Al-Muwayhi MA, Assaeed AM, Migdadi HM Tags: Mol Biol Rep Source Type: research

New insights on the role of autophagy in the pathogenesis and treatment of melanoma.
Abstract Despite the depth of knowledge concerning the pathogenesis of melanoma, the most aggressive type of skin cancer, the prognosis and survival of patients still remain a challenge. In addition, responses to chemotherapy and immunotherapy are still poor, which underscore an urgent need in the development of new therapeutic strategies for the treatment of melanoma. Recently, the dynamic role of autophagy has gained considerable interest in the pathogenesis and treatment of melanoma. Whereas a decrease in autophagy activity promotes melanoma formation by increasing oncogene-induced tumorigenesis and DNA damage ...
Source: Molecular Biology Reports - October 9, 2020 Category: Molecular Biology Authors: Rahmati M, Ebrahim S, Hashemi S, Motamedi M, Moosavi MA Tags: Mol Biol Rep Source Type: research

Transcription factor Kruppel-like factor 5 positively regulates the expression of AarF domain containing kinase 4.
Abstract AarF domain containing kinase 4 (ADCK4) is identified as a candidate gene associated with hereditary nephrotic syndrome (NS). Kruppel-like factor 5 (KLF5) is reported to promote podocyte survival by blocking the ERK/p38 MAPK pathways. Both ADCK4 and KLF5 are involved in the occurrence and development of podocyte disease, but their interaction remains unclear. Firstly, we found that the mRNA levels of ADCK4 and KLF5 decreased in NS patients, and both levels showed an obvious linear relationship. Secondly, we cloned the ADCK4 promoter region and examined its promoter activity in Hela, A549, and HEK 293 cell...
Source: Molecular Biology Reports - October 8, 2020 Category: Molecular Biology Authors: Chen X, Liu S, Chen J, Wang X, Zhou G Tags: Mol Biol Rep Source Type: research

Liquid biopsy approaches for pleural effusion in lung cancer patients.
Abstract Genomic profiling of tumors has become the mainstay for diagnosis, treatment monitoring and a guide to precision medicine. However, in clinical practice, the detection of driver mutations in tumors has several procedural limitations owing to progressive disease and tumor heterogeneity. The current era of liquid biopsy promises a better solution. This diagnostic utility of liquid biopsy has been demonstrated by numerous studies for the detection of cell-free DNA (cfDNA) in plasma for disease diagnosis, prognosis, and prediction. However, cfDNAs are limited in blood circulation and still hurdles to achieve ...
Source: Molecular Biology Reports - October 7, 2020 Category: Molecular Biology Authors: Baburaj G, Damerla RR, Udupa KS, Parida P, Munisamy M, Kolesar J, Rao M Tags: Mol Biol Rep Source Type: research

Sequence comparison of the mitochondrial genomes in two species of the genus Nerita (Gastropoda: Neritimorpha: Neritidae): phylogenetic implications and divergence time estimation for Neritimorpha.
This study contributes towards the comparative mitogenomic analysis of Neritidae and phylogenetic considerations among Neritimorpha species. The estimation of divergence time revealed that the two Nerita species were differentiated in the late Paleogene of the Cenozoic Era, and their evolution may be related to environmental changes. PMID: 33029703 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 7, 2020 Category: Molecular Biology Authors: Feng J, Guo Y, Yan C, Ye Y, Li J, Guo B, Lü Z Tags: Mol Biol Rep Source Type: research

The role of exogenous Fibrinogen in cardiac surgery: stop bleeding or induce cardiovascular disease.
Abstract The surgical treatment contributes to broad variety of cardiovascular diseases (CVD). Due to many involved factors in preoperative bleeding, it is almost difficult to perform better Haemostatic approach. Fibrinogen is a major blood glycoprotein and a coagulation factor which decreases postoperative bleeding. It has a potential role in platelet activation and bleeding inhibition; it may reflect the inflammatory responses and be related to the endothelial dysfunction. Fibrinogen can act as a pro-inflammatory element via increasing some inflammatory markers including IL-6, tumor necrosis factor-α (TNF-...
Source: Molecular Biology Reports - October 7, 2020 Category: Molecular Biology Authors: Zadeh FJ, Mohammadtaghizadeh M, Bahadori H, Saki N, Rezaeeyan H Tags: Mol Biol Rep Source Type: research

Recent advances in the nucleic acid-based diagnostic tool for coronavirus.
Abstract Recently in China, a novel coronavirus outbreak took place which caused pneumonia-like symptoms. This coronavirus belongs to the family of SARS and MERS and causes respiratory system disease known as COVID-19. At present we use polymerase chain reaction (PCR) based molecular biology methods for the detection of coronavirus. Other than these PCR based methods, some improved methods also exist such as microarray-based techniques, Real time-quantitative PCR, CRISPR-Cas13 based tools but almost all of the available methods have advantages and disadvantages. There are many limitations associated with this meth...
Source: Molecular Biology Reports - October 6, 2020 Category: Molecular Biology Authors: Prabhakar PK, Lakhanpal J Tags: Mol Biol Rep Source Type: research

Nitric oxide induces HepG2 cell death via extracellular signal-regulated protein kinase activation by regulating acid sphingomyelinase.
Abstract Nitric oxide (NO) plays a vital role in the occurrence and development of tumours. Acid sphingomyelinase (ASM) participates in cell apoptosis, cell proliferation, metabolism and other biological processes. However, whether ASM has an effect on NO-treated HepG2 cells remains unknown, and the role of the extracellular signal-regulated protein kinase (ERK) pathway is also unclear. In the present study, the effects of NO on cell viability and apoptosis were assayed, followed by investigating the mRNA and protein levels of ASM and ERK phosphorylation in NO-treated HepG2 cells. The results showed that diethylen...
Source: Molecular Biology Reports - October 6, 2020 Category: Molecular Biology Authors: Zhang L, Dai J, Zeng Z, Jia Y Tags: Mol Biol Rep Source Type: research

The effect of vitamin E and selenium combination in repairing fluoride-induced DNA damage to NRK-52E cells.
This study aims to determine the extent of NaF-induced DNA damage and to investigate the effect of vitamin E and selenium combination (ES) in preventing and repairing this damage. For this purpose, we administered different combinations of NaF and ES to NRK-52E cells and determined the effective concentrations of ES and the NaF IC50 values associated with different incubation times (3, 12, and 24 h) by using the MTT assay. The determined quantities of NaF IC50 in association with time and the NaF IC50 + ES combination were administered to the cells. The extent of DNA damage was determined with the comet assay and the expre...
Source: Molecular Biology Reports - October 6, 2020 Category: Molecular Biology Authors: Yüksek V, Çetin S, Usta A Tags: Mol Biol Rep Source Type: research

Curcumin induces chemosensitization to doxorubicin in Duke's type B coloadenocarcinoma cell line.
Abstract Cancer cells require higher levels of ATP for their sustained growth, proliferation, and chemoresistance. Mitochondrial matrix protein, C1qbp is upregulated in colon cancer cell lines. It protects the mitochondria from oxidative stress, by inhibiting the Membrane Permeability Transition (MPT) pore and providing uninterrupted synthesis of ATP. This intracellular interaction of C1qbp could be involved in chemoresistance development. Natural chemosensitizing agent, curcumin has been used in the treatment of multiple cancers. In this current study, we elucidate the role of C1qbp during curcumin induced chemos...
Source: Molecular Biology Reports - October 6, 2020 Category: Molecular Biology Authors: Jayarajan J, Angandoor S, Vedulla SH, Sritharan S, Ganesan K, War AR, Sivalingam N Tags: Mol Biol Rep Source Type: research

A novel freshwater cyanophage vB_MelS-Me-ZS1 infecting bloom-forming cyanobacterium Microcystis elabens.
This study isolated and characterized a novel broad-host-range Microcystis phage Me-ZS1 presenting a genetically distinct clade of freshwater cyanophage. The features of cyanophage Me-ZS1 provide a potential solution to the loss caused by cyanobacterial bloom. PMID: 33025507 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 6, 2020 Category: Molecular Biology Authors: Lin W, Li D, Sun Z, Tong Y, Yan X, Wang C, Zhang X, Pei G Tags: Mol Biol Rep Source Type: research

Distinct roles for Notch1 and Notch3 in human adipose-derived stem/stromal cell adipogenesis.
This study deepens the understanding of the Notch pathway by clarifying the distinct roles of Notch1 and Notch3 during adipogenesis. We showed that Notch3 is involved in early adipogenic differentiation, while Notch1 functions later in the process. In addition, we begin to uncover the interaction between the Notch and Wnt signaling pathways that may offer novel therapeutic targets aimed at obesity and diabetes. PMID: 33021719 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - October 5, 2020 Category: Molecular Biology Authors: Liu MC, Logan H, Newman JJ Tags: Mol Biol Rep Source Type: research

Investigation of 8-methoxy-3-(4-nitrobenzoyl)-6-propyl-2H-chromen-2-one as a promising coumarin compound for the development of a new and orally effective antileishmanial agent.
This study investigated the in vitro and in vivo leishmanicidal activity of synthetic coumarin compounds (C1-C5) in parasites Leishmania (L.) amazonensis and L. (L.) infantum chagasi. The cytotoxicity of these compounds in mammalian cells and their influence on production of reactive oxygen species was also investigated. In vitro assays showed that 8-methoxy-3-(4-nitrobenzoyl)-6-propyl-2H-chromen-2-one (C4) was as active as AMP B mainly in the amastigote form (p 
Source: Molecular Biology Reports - October 5, 2020 Category: Molecular Biology Authors: de Figueiredo Peloso E, Merli RJ, Espuri PF, Nunes JB, Colombo FA, Sierra EJT, de Paulo DC, Dos Santos MH, Carvalho DT, Marques MJ Tags: Mol Biol Rep Source Type: research

Functional characterization of asnC family transcriptional regulator in Pseudomonas aeruginosa PGPR2 during root colonization.
In this study, we constructed isogenic mutant of asnC family transcriptional regulator encoded by PGPR2_17510 by allele exchange mutagenesis. The ΔasnC mutant was able to efficiently colonize corn roots with a twofold increase in population when compared to the wild-type strain. Similarly, the mutant strain outcompeted the wild-type strain in a competition assay, where the mutant strain represented 90% of the total population recovered from the root. We compared the whole transcriptome of the wild-type and the ΔasnC mutant of P. aeruginosa PGPR2 when exposed to the corn root exudates. The RNA-Seq revealed that ...
Source: Molecular Biology Reports - October 3, 2020 Category: Molecular Biology Authors: Sivakumar R, Gunasekaran P, Rajendhran J Tags: Mol Biol Rep Source Type: research

Methylphenidate and Rosmarinus officinalis improves cognition and regulates inflammation and synaptic gene expression in AlCl3-induced neurotoxicity mouse model.
In this study we elucidated the neuroprotective and anti-inflammatory effects of MPH and Rosmarinus officinalis (rosemary) extract, an ancient aromatic herb with several applications in traditional medicine. Briefly, six groups of mice (n = 8 each group), were specified for the study and behavioral analysis was performed to analyze spatial memory followed by histological assessment and gene expression analysis of synaptic (Syn I, II and III) and inflammatory markers (IL-6, TNFα and GFAP) via qRT-PCR, in an AlCl3-induced mouse model for neurotoxicity. The behavioral analysis demonstrated significant cogn...
Source: Molecular Biology Reports - October 3, 2020 Category: Molecular Biology Authors: Khalid A, Abbasi UA, Amber S, Sumera, Mirza FJ, Asif M, Javed A, Zahid S Tags: Mol Biol Rep Source Type: research