Pancreatic ductal adenocarcinomas from Mexican patients present a distinct genomic mutational pattern.
gla J Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers in humans, with less than 5% 5-year survival rate. PDAC is characterized by a small number of recurrent mutations, including KRAS, CDKN2A, TP53, and SMAD4 and a long "tail" of infrequent mutated genes. Most of the studies have been performed in US and European populations, so new studies are needed to describe the mutational landscape of these tumors in other cohorts. The present study analyzed the exome and transcriptome of four PDAC tumors from Mexican patients. We found a paucity of the previously described recurren...
Source: Molecular Biology Reports - June 24, 2020 Category: Molecular Biology Authors: Sanchez P, Espinosa M, Maldonado V, Barquera R, Belem-Gabiño N, Torres J, Cravioto A, Melendez-Zajgla J Tags: Mol Biol Rep Source Type: research
Novel alternatively-spliced exons of the VRK2 gene in mouse brain and microglial cells.
Abstract Common sequence variations in the VRK2 gene contribute to genetic risk for various psychiatric diseases including schizophrenia and major depressive disorder. Despite the clear importance of studying the regulation and function of VRK2 for understanding the causes of these diseases, the organisation and expression of the gene remain poorly characterised. Using reverse-transcriptase-PCR, we have amplifed exons of Vrk2 mRNA from regions of mouse brain, and from different cell classes comprising neurones, astrocytes and microglial cells. We find that Vrk2 mRNA is expressed in all cell types, and that the spl...
Source: Molecular Biology Reports - June 24, 2020 Category: Molecular Biology Authors: Almarzooq S, Kwon J, Willis A, Craig J, Morris BJ Tags: Mol Biol Rep Source Type: research
The effects of hybridization and genome doubling in plant evolution via allopolyploidy.
Abstract Polyploidy is a pervasive and recurring phenomenon across the tree of life, which occurred at variable time scales, ecological amplitudes and cell types, and is especially prominent in the evolutionary histories of plants. Importantly, many of the world's most important crops and noxious invasive weeds are recent polyploids. Polyploidy includes two major types, autopolyploidy, referring to doubling of a single species genome, and allopolyploidy referring to doubling of two or more merged genomes via biological hybridization of distinct but related species. The prevalence of both types of polyploidy implie...
Source: Molecular Biology Reports - June 23, 2020 Category: Molecular Biology Authors: Qiu T, Liu Z, Liu B Tags: Mol Biol Rep Source Type: research
Genetic diversity analysis for narrow-leafed lupin (Lupinus angustifolius L.) by SSR markers.
In this study, we assessed the genetic diversity among a set of 109 newly introduced accessions of narrow-leafed lupin using 76 genomic SSR markers. Data analysis suggested that the average gene diversity index and average polymorphism information content (PIC) were 0.4758 and 0.4328, respectively. The mean allele number per loci (Na) was 6.3816. The population structure analysis identified two subgroups based on delta K (ΔK) values. This result is in accordance with that of a PCA. The AMOVA analysis showed that most of molecular variance were within population. These results will be useful to guide the genetic impro...
Source: Molecular Biology Reports - June 23, 2020 Category: Molecular Biology Authors: Ji Y, Liu R, Hu J, Huang Y, Wang D, Li G, Rahman MM, Zhang H, Wang C, Li M, Yang T, Zong X Tags: Mol Biol Rep Source Type: research
Development and optimisation of molecular assays for microsatellite genotyping and molecular sexing of non-invasive samples of the ghost bat, Macroderma gigas.
This study highlights the value of our molecular assays as a potential capture-mark-recapture technique for population monitoring for this species. PMID: 32577991 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 23, 2020 Category: Molecular Biology Authors: Ottewell K, Thavornkanlapachai R, McArthur S, Spencer PBS, Tedeschi J, Durrant B, Knuckey C, Armstrong K, Byrne M Tags: Mol Biol Rep Source Type: research
Genome-wide AP2/ERF gene family analysis reveals the classification, structure, expression profiles and potential function in orchardgrass (Dactylis glomerata).
This study systematic explored the DgERFs at the genome level for the first time, which lays a foundation for a better understanding of AP2/ERF gene function in Dactylis glomerata and other types of forage. PMID: 32577992 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 23, 2020 Category: Molecular Biology Authors: Xu L, Feng G, Yang Z, Xu X, Huang L, Yang Q, Zhang X Tags: Mol Biol Rep Source Type: research
The correlation of combined OGG1, CYP1A1 and GSTP1 gene variants and risk of lung cancer of male Iraqi waterpipe tobacco smokers.
Abstract Genetic polymorphisms of genes whose products are responsible for activities, such as xenobiotic metabolism, mutagen detoxification and DNA-repair, have been predicted to be associated with the risk of developing lung cancer (LC). The association of LC with tobacco smoking has been extensively investigated, but no studies have focused on the Arab ethnicity. Previously, we examined the association between genetic polymorphisms among Phase I and Phase II metabolism genes and the risk of LC. Here, we extend the data by examining the correlation of OGG1 Ser326Cys combined with CYP1A1 (Ile462Val and MspI) and ...
Source: Molecular Biology Reports - June 23, 2020 Category: Molecular Biology Authors: Kudhair BK, Alabid NN, Zayed KS, Lafta IJ, Taheri-Kafrani A Tags: Mol Biol Rep Source Type: research
Bioassay-based detection of Toxoplasma gondii in free-range chickens from Khorramabad, Iran: comparison of direct microscopy and semi-nested PCR.
This study aimed to investigate the occurrence of anti-Toxoplasma gondii antibodies in free-range chickens from Khorramabad, western Iran, and also to compare the performance of direct microscopy and semi-nested PCR in mice bioassayed with tissues from seropositive chickens. We investigated 97 serum samples from free-range chickens, using the modified agglutination test (MAT). Tissues from all seropositive chickens (MAT ≥ 1:10) were bioassayed in mice. All inoculated mice were examined by direct microscopy and a semi-nested PCR targeting the 529 bp repeat element (RE) of the parasite. Anti-T. gondii a...
Source: Molecular Biology Reports - June 23, 2020 Category: Molecular Biology Authors: Alizadeh-Sarabi Z, Pasandideh Z, Shokrani H, Dezfoulian O Tags: Mol Biol Rep Source Type: research
Parthenolide ameliorates tweak-induced podocytes injury.
This study aimed to explore the effect and the underlying mechanism of PTL on the anti-apoptotic and anti- inflammatory ability of tweak-induced podocytes. Conditionally immortalized mouse podocytes were incubated with Tumor necrosis factor-like weak inducer of apoptosis (Tweak, 100 ng/ml), PTL(10 µM) or Tweak + PTL for 12 h, 24 and 48 h, respectively. Podocytes viability was detected by CCK-8 assay. Tweak and Cxcl16 expression were evaluated by western blot and immunofluorescence assay. Dil-oxLDL stain was detected by immunofluorescence analysis. Intracellular Total Cholesterol (TC) content was measure...
Source: Molecular Biology Reports - June 22, 2020 Category: Molecular Biology Authors: Yu L, Zhang C, Chen Y, Li Q, Wang J, Sun S Tags: Mol Biol Rep Source Type: research
Curcumin attenuates IL-17A mediated pulmonary SMAD dependent and non-dependent mechanism during acute lung injury in vivo.
Abstract Acute lung injury (ALI) is a pathologic condition responsible for incurable human chronic respiratory diseases. Recent studies have shown the involvement of the glycoprotein, IL17A secreted by IL-17 producing cells in chronic inflammation. The current investigation was carried out to study the IL-17A mediated activation of SMAD and non- SMAD signaling in alveolar epithelial cells and to assess the putative modulatory role of curcumin. C57BL/6 mice were exposed to IL-17A and curcumin was administered as an intervention to modulate the IL-17A-induced alveolar damage. Techniques like Immunofluorescence and r...
Source: Molecular Biology Reports - June 22, 2020 Category: Molecular Biology Authors: Shaikh SB, Bhat SG, Bhandary YP Tags: Mol Biol Rep Source Type: research
The role of molecular mechanism of Ten-Eleven Translocation2 (TET2) family proteins in pathogenesis of cardiovascular diseases (CVDs).
Abstract Cardiovascular disease (CVD) is one of the most common diseases worldwide. The underlying pathogenesis of the disease has not yet been determined, but many factors have been identified. Tet methylcytosine dioxygenase 2 (TET2) is one of the epigenetic factors involved in regulating many genes. Therefore, based on the studies shown, this factor plays an important role in preventing the occurrence of CVD. TET2 has been shown to increase angiogenesis by expressing Robo4. It also increases the activity of Matrix metalloproteinases (MMPs) and stimulates the secretion of Vascular endothelial growth factor angiog...
Source: Molecular Biology Reports - June 22, 2020 Category: Molecular Biology Authors: Zadeh FJ, Akbari T, Zayeri ZD, Samimi A, Davari N, Rezaeeyan H Tags: Mol Biol Rep Source Type: research
Genetic associations and serum paraoxonase levels with atherosclerosis in western Iranian patients.
In this study, blood specimens were collected from 145 healthy individuals and 154 patients with atherosclerosis proven by angiography referred to Shahid Madani Hospital, Khorramabad, Iran. Genomic deoxy ribonucleic acid (DNA) was extracted from whole blood. For all the subjects, restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was carried out for the detection of L55M and Q192R polymorphisms. PON1 enzyme activity and the level of oxLDL were also evaluated. There was a 3.114-fold increase in the risk of developing atherosclerosis in the subjects presenting the PON1L55M, MM genotype compared to ...
Source: Molecular Biology Reports - June 21, 2020 Category: Molecular Biology Authors: Shahsavari G, Nouryazdan N, Adibhesami G, Birjandi M Tags: Mol Biol Rep Source Type: research
The effects of the neurotoxic agent emamectin benzoate on the expression of immune and stress-related genes and blood serum profiles in the Rainbow trout.
Abstract Emamectin, a neurotoxic agent, is a semi-synthetic insecticide that belongs to the Avermectin family and is used against helmintic infections in the Salmonidae family. Its secondary effects are not clear; thus, the aim of this study was to investigate the only effects of emamectin benzoate on various biochemical parameters (AST, ALT, GGT, total protein, albumin and glucose) in serum and expressional changes of IL-1β, TNF-α, HSP70 and IL-8 in liver and spleen. For the purpose stated above, rainbow trout (n = 15) were administered 50 μg EB per kg fish daily for 7, 14 an...
Source: Molecular Biology Reports - June 21, 2020 Category: Molecular Biology Authors: Kilercioglu S, Ay O, Oksuz H, Yilmaz MB Tags: Mol Biol Rep Source Type: research
LncRNAs and circular RNAs as endothelial cell messengers in hypertension: mechanism insights and therapeutic potential.
Abstract Endothelial cells are major constituents in the vasculature, and they act as important players in vascular homeostasis via secretion/release of vasodilators and vasoconstrictors. In healthy arteries, endothelial cells play a key role in the regulation of vascular tone, cellular adhesion, and angiogenesis. A shift in the functions of the blood vessels toward vasoconstriction, proinflammatory state, oxidative stress and deficiency of nitric oxide (NO) might lead to endothelial dysfunction, a key event implicated in the pathophysiology of cardiovascular metabolic diseases, including diabetes, atherosclerosis...
Source: Molecular Biology Reports - June 21, 2020 Category: Molecular Biology Authors: Zhang JR, Sun HJ Tags: Mol Biol Rep Source Type: research
Proteomic analyses unraveling water stress response in two Eucalyptus species originating from contrasting environments for aridity.
e; AC Abstract Eucalyptus are widely cultivated in several regions of the world due to their adaptability to different climatic conditions and amenable to tree breeding programs. With changes in environmental conditions pointing to an increase in aridity in many areas of the globe, the demand for genetic materials that adapt to this situation is required. Therefore, the aim of this work was to identify contrasting differences between two Eucalyptus species under water stress through the identification of differentially abundant proteins. For this, total protein extraction was proceeded from leaves of both species ...
Source: Molecular Biology Reports - June 20, 2020 Category: Molecular Biology Authors: Martins RS, Faria JMR, Rossini BC, Marino CL, Dos Santos LD, José AC Tags: Mol Biol Rep Source Type: research
The interplay between oxidative stress and bioenergetic failure in neuropsychiatric illnesses: can we explain it and can we treat it?
Abstract Nitro-oxidative stress and lowered antioxidant defences play a key role in neuropsychiatric disorders such as major depression, bipolar disorder and schizophrenia. The first part of this paper details mitochondrial antioxidant mechanisms and their importance in reactive oxygen species (ROS) detoxification, including details of NO networks, the roles of H2O2 and the thioredoxin/peroxiredoxin system, and the relationship between mitochondrial respiration and NADPH production. The second part highlights and identifies the causes of the multiple pathological sequelae arising from self-amplifying increases in ...
Source: Molecular Biology Reports - June 20, 2020 Category: Molecular Biology Authors: Morris G, Walder KR, Berk M, Marx W, Walker AJ, Maes M, Puri BK Tags: Mol Biol Rep Source Type: research
Construction of an expression vector mediated by the dual promoter for prokaryotic and mammalian cell expression system.
In conclusion, a prokaryotic-eukaryotic double expression vector was successfully constructed, which has potential applications in rapid cloning and expression of recombinant proteins in both prokaryotic and eukaryotic expression systems. PMID: 32564228 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 20, 2020 Category: Molecular Biology Authors: Yi DD, Wang XY, Zhang WL, Wang M, Zhang JH, Wang TY Tags: Mol Biol Rep Source Type: research
Development of genome wide transposable elements based repeat junction markers in Jatropha (Jatropha curcas L.).
In this study, we have utilized transposable elements' (TE) repeat junctions (RJs) which are an important constituent of the genome, used to form a genome-wide molecular marker platform owing to its use in genomic studies of plants. We screened our previously generated Jatropha hybrid genome assembly of size 265 Mbp using RJPrimers pipeline software and identified a total of 1274 TE junctions. For the predicted RJs, we designed 2868 polymerase chain reaction (PCR) based RJ markers (RJMs) flanking the junction regions. In addition to marker design, the identified RJs were utilized to detect 225,517 TEs across the genom...
Source: Molecular Biology Reports - June 19, 2020 Category: Molecular Biology Authors: Yepuri V, Jalali S, Kancharla N, Reddy VB, Arockiasamy S Tags: Mol Biol Rep Source Type: research
Evaluation of a cell-based osteogenic formulation compliant with good manufacturing practice for use in tissue engineering.
J, Vives J Abstract Proper bony tissue regeneration requires mechanical stabilization, an osteogenic biological activity and appropriate scaffolds. The latter two elements can be combined in a hydrogel format for effective delivery, so it can readily adapt to the architecture of the defect. We evaluated a Good Manufacturing Practice-compliant formulation composed of bone marrow-derived mesenchymal stromal cells in combination with bone particles (Ø = 0.25 to 1 µm) and fibrin, which can be readily translated into the clinical setting for the treatment of bone defects, as an alternat...
Source: Molecular Biology Reports - June 19, 2020 Category: Molecular Biology Authors: Vivas D, Grau-Vorster M, Oliver-Vila I, García-López J, Vives J Tags: Mol Biol Rep Source Type: research
Polymorphisms in XPC and XPD genes modulate DNA damage in pesticide-exposed agricultural workers of Punjab, North-West India.
Abstract The genetic susceptibility of individuals to the genotoxic effect of pesticides may be modulated by variations in genes involved in nucleotide excision repair (NER) pathway and therefore plays an important role in the evaluation of occupational risk. We aimed to evaluate the role of xeroderma pigmentosum complementation group C (XPC) Lys939Gln (A2920C, rs2228001), XPC Ala499Val (C2151T, rs2228000), xeroderma pigmentosum complementation group D (XPD) Asp312Asn (G23591A, rs1799793) and XPD Lys751Gln (A35931C, rs13181) in the modulation of DNA damage. A total of 450 subjects (225 pesticide-exposed agricultur...
Source: Molecular Biology Reports - June 19, 2020 Category: Molecular Biology Authors: Kaur K, Kaur R Tags: Mol Biol Rep Source Type: research
Biotic stress triggered small RNA and RNAi defense response in plants.
Abstract The yield of crops is largely affected by different types of biotic stresses. To minimize the damage, crop plants adapted themselves to overcome the stress conditions through gene expression reprogramming at transcriptional and post-transcriptional levels. With a better knowledge of plants' responses in adverse environments, new methodologies and strategies have been applied to develop better stress-tolerant plants. In this manner, small RNAs (micro RNA and small-interfering RNA) are reported to play a central role to combat biotic stresses in plants. Depending upon the stress stimuli, these small RNAs ca...
Source: Molecular Biology Reports - June 19, 2020 Category: Molecular Biology Authors: Ali M, Javaid A, Naqvi SH, Batcho A, Kayani WK, Lal A, Sajid IA, Nwogwugwu JO Tags: Mol Biol Rep Source Type: research
Effects of diets high in corn oil or in extra virgin olive oil on oxidative stress in an experimental model of breast cancer.
Abstract Experimental evidence highlights the importance of dietetic factors on breast cancer. In this work we aimed to analyze the effects two oils, corn oil (rich in n-6 polyunsaturated fatty acids -PUFA-) and extra virgin olive oil (EVOO), on oxidative stress in an animal model of breast carcinogenesis. Female rats were fed a low-fat control, a high-corn oil, or a high-EVOO diet from weaning or after induction with 7,12-dimethylbenz[a]anthracene at 53 days. Animals were euthanized at 36, 51, 100 and 246 days of age. We analyzed antioxidant enzymes (mRNA and activity of superoxide dismutase, glutathione peroxida...
Source: Molecular Biology Reports - June 18, 2020 Category: Molecular Biology Authors: Escrich R, Vela E, Solanas M, Moral R Tags: Mol Biol Rep Source Type: research
Isolation and characterization of microsatellites for the neotropical dioecious palm Chamaedorea tepejilote (Arecaceae) and cross-amplification in other Chamaedorea species.
Abstract Palms are important components of tropical and subtropical ecosystems and have even been considered keystone plant resources that can support a large array of pollinators and frugivores. Palms are also economically important. Chamaedorea tepejilote Liebm. is a widely distributed palm with important bioeconomic potential for food, traditional medicine and ornamental purposes. Eighteen microsatellite primers were developed for C. tepejilote. Polymorphism and genetic diversity were evaluated in 71 individuals from four populations in Costa Rica. Thirteen loci were polymorphic and the number of alleles in the...
Source: Molecular Biology Reports - June 18, 2020 Category: Molecular Biology Authors: Fuchs EJ, Cascante-Marin A, Madrigal-Brenes R, Harvey N, Quesada M Tags: Mol Biol Rep Source Type: research
MetaRNAseq analysis of surti buffalo rumen content reveals that transcriptionally active microorganisms need not be abundant.
Abstract The present study describes rumen microbiota composition and their functional profiles in Indian Surti buffaloes by metagenomic (MG) and metatranscriptomic (MT) approaches. The study compares samples from buffaloes fed three different proportion of roughages; green and dry type of roughage; and different rumen liquor fractions. Irrespective of sample, Bacteroidetes and Firmicutes were the most predominant bacterial phyla, followed by Proteobacteria, Fibrobacteres and Actinobacteria while, Prevotella, Bacteroides, Ruminococcus and Clostridium were the most abundant genera. Different proportions of taxa wer...
Source: Molecular Biology Reports - June 16, 2020 Category: Molecular Biology Authors: Hinsu AT, Patel AB, Pandit RJ, Thakkar JR, Shah RK, Jakhesara SJ, Koringa PG, Joshi CG Tags: Mol Biol Rep Source Type: research
Anti-inflammatory Activity of Bioactive Compounds from Microalgae and Cyanobacteria by Focusing on the Mechanisms of Action.
Abstract Microalgae and cyanobacteria are the potentially valuable source of bioactive compounds applied in the various industries and human usage in different fields of pharmaceutical, nutraceutical, and cosmetic disciplines. One of the interesting aspects is their application as the anti-inflammatory agents for treatment of inflammation related mal-conditions. Natural compounds are of great importance in the treatment of inflammations to reduce the reaction of immune system against pathogens, toxic compounds and damaged cells. A wide range of different metabolites with various chemical structures, including smal...
Source: Molecular Biology Reports - June 15, 2020 Category: Molecular Biology Authors: Tabarzad M, Atabaki V, Hosseinabadi T Tags: Mol Biol Rep Source Type: research
Metformin and/or low dose radiation reduces cardiotoxicity and apoptosis induced by cyclophosphamide through SIRT-1/SOD and BAX/Bcl-2 pathways in rats.
Conclusions: The results revealed that MET and/or LDR attenuate CP-induced cardiotoxicity by inhibiting oxidative stress and preserving the activity of antioxidant enzymes through SIRT-1/SOD and BAX/Bcl-2 pathways. PMID: 32537703 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 14, 2020 Category: Molecular Biology Authors: El Kiki SM, Omran MM, Mansour HH, Hasan HF Tags: Mol Biol Rep Source Type: research
Proangiogenic signature in advanced laryngeal carcinoma after microRNA expression profiling.
Abstract The aim of this study is to evaluate which dysregulated angiomiRs compose the specific proangiogenic microRNA signature of advanced laryngeal cancer and review the literature. Thirty-six samples from twelve patients with advanced laryngeal carcinoma were collected. Total RNA was extracted and microRNA global profiling was performed using Agilent Technologies Microarray Kit. Fifty-nine microRNAs were found to have significantly different expression levels. Eleven microRNAs from the whole group were sorted as regulators of tumor angiogenesis (angiomiRs): seven were up-regulated-miR-1246, miR-181b 5p, miR-18...
Source: Molecular Biology Reports - June 12, 2020 Category: Molecular Biology Authors: Popov TM, Giragosyan S, Petkova V, Stancheva G, Marinov T, Belitova M, Rangachev J, Popova D, Kaneva RP Tags: Mol Biol Rep Source Type: research
Protective effect of Nasturtium officinale R. Br and quercetin against cyclophosphamide-induced hepatotoxicity in rats.
Abstract Cyclophosphamide (CPA) is used in the management of autoimmune conditions and malignant illnesses. However, its therapeutic use is limited because of its severe side effects, especially hepatotoxicity attributed to oxidative stress. Nasturtium officinale R. Br (watercress or WC) has pharmacological properties, such as anti-inflammation, and antioxidant activities. Therefore, the present study was design to assess effects of WC or its active ingredient, quercetin (QE), against CPA-induced hepatotoxicity. For this study, 49 male Wistar rats (200-250 g) were randomly selected and categorized into seven ...
Source: Molecular Biology Reports - June 12, 2020 Category: Molecular Biology Authors: Doustimotlagh AH, Kokhdan EP, Vakilpour H, Khalvati B, Barmak MJ, Sadeghi H, Asfaram A Tags: Mol Biol Rep Source Type: research
Expression analysis of defense-related genes in cucumber (Cucumis sativus L.) against Phytophthora melonis.
Abstract Phytophthora melonis is one of the most destructive cucumber disease, causing severe economic losses in the globe. Despite intense research efforts made in the past years, no permanent cure currently exists for this disease. With the aim to understand the molecular mechanisms of defense against P. melonis, root collars and leaves of four cucumber genotypes consisting of resistant Ramezz; moderately resistant Baby, and very susceptible Mini 6-23 and Extrem, were monitored for quantitative gene expression analysis of the five antifungal and/or anti-oomycete genes (CsWRKY20, CsLecRK6.1, PR3, PR1-1a and LOX1)...
Source: Molecular Biology Reports - June 12, 2020 Category: Molecular Biology Authors: Hashemi L, Golparvar AR, Nasr-Esfahani M, Golabadi M Tags: Mol Biol Rep Source Type: research
Genetic diversity of Biomphalaria pfeifferi, the intermediate host of Schistosoma mansoni in Shamva district, Zimbabwe: role on intestinal schistosomiasis transmission.
Abstract The fresh water snail Biomphalaria pfeifferi is the intermediate host for Schistosoma mansoni, which causes human intestinal schistosomiasis in Zimbabwe. Despite the medical importance of this intermediate host, there are no current data on its molecular characterization in Zimbabwe. In 2016, human water contact sites were identified in four communities in Madziwa area, Shamva district, Zimbabwe. The survey sites were recorded and mapped using a global positioning system. A 655 bp region of the mitochondrial cytochrome oxidase subunit I gene was amplified in 70 B. pfeifferi snails. The sequence data ...
Source: Molecular Biology Reports - June 12, 2020 Category: Molecular Biology Authors: Mutsaka-Makuvaza MJ, Zhou XN, Tshuma C, Abe E, Manasa J, Manyangadze T, Allan F, Chin'ombe N, Webster B, Midzi N Tags: Mol Biol Rep Source Type: research
Regulation of biofilm formation by marT in Salmonella Typhimurium.
In this study, we aimed at identifying the regulatory role of marT gene, known as the regulator of misL, on 15 different biofilm-related genes in S. Typhimurium 14028 strain. We also tested the strains for their ability to form biofilm and determined the adherence characteristics of the wild type and the mutant strains of the organism on Caco-2 and HEp-2 cells. For comparative analyses of the candidate genes, individual gene mutations were created via antibiotic gene cassette insertion into each gene of interest. marT gene was cloned behind an arabinose inducible BAD promoter in order to control marT expression. This recom...
Source: Molecular Biology Reports - June 11, 2020 Category: Molecular Biology Authors: Eran Z, Akçelik M, Yazıcı BC, Özcengiz G, Akçelik N Tags: Mol Biol Rep Source Type: research
Inhibition of suicidal erythrocyte death by pyrogallol.
Abstract Pyrogallol, a polyphenolic component of Acacia nilotica has previously been reported to induce apoptosis of diverse cell types. Pyrogallol is in part effective by influencing gene expression and by interference with mitochondrial function. Despite lack of nuclei and mitochondria, erythrocytes may undergo eryptosis, a suicidal death apparent from phosphatidylserine translocation to the erythrocyte surface and cell shrinkage. Eryptosis is triggered by glucose depletion, by oxidation, by hyperosmotic cell shrinkage and by excessive Ca2+ entry. As enhanced eryptosis is a common cause of anemia, uncovering inh...
Source: Molecular Biology Reports - June 10, 2020 Category: Molecular Biology Authors: Liu J, Bhuyan AAM, Ma K, Zhang S, Cheng A, Lang F Tags: Mol Biol Rep Source Type: research
Correction to: MicroRNA signature in hepatocellular carcinoma patients: identification of potential markers.
Abstract The correct spelling of the 7th authors' name is Mona Watany. PMID: 32524387 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 10, 2020 Category: Molecular Biology Authors: Elhendawy M, Abdul-Baki EA, Abd-Elsalam S, Hagras MM, Zidan AA, Abdel-Naby AY, Watany M, Elkabash IA, Salem ML, Elshanshoury M, Soliman S, Abdou S Tags: Mol Biol Rep Source Type: research
Population connectivity and genetic structure of Asian green mussel, Perna viridis along Indian waters assessed using mitochondrial markers.
Abstract Perna viridis (Linnaeus, 1758), the Asian green mussel, belonging to the family Mytilidae is widely distributed along the Indian coast. The species is majorly found in southeastern countries and is considered an ideal candidate for aquaculture due to its high nutritional value and growth rate. Obtaining their genetic information is essential for their sustainable capture-based production. In the present study, genetic variation, population structure, and demographic processes of the populations across the distribution of this species were assessed using the mitochondrial DNA ATPase6 and cytb gene. In tota...
Source: Molecular Biology Reports - June 10, 2020 Category: Molecular Biology Authors: Divya PR, Jency PME, Joy L, Kathirvelpandian A, Singh RK, Basheer VS Tags: Mol Biol Rep Source Type: research
Molecular identification of Trichocera maculipennis, an invasive fly species in the Maritime Antarctic.
Abstract Trichocera maculipennis, an invasive Diptera, was described for the first time in Antarctica in 2006 in a sewage system of one of the scientific stations on King George Island, South Shetland Islands, and started to increase its distribution within the island. To date, only taxonomical description of this species, based on morphological data has been available, as there were no molecular data recorded. In the present study, we present two methods of molecular identification of this species-based on partial cytochrome c oxidase subunit I (COI) and 16S ribosomal RNA (16S) genes. An appropriate and easy-to-u...
Source: Molecular Biology Reports - June 10, 2020 Category: Molecular Biology Authors: Potocka M, Krzemińska E, Gromadka R, Gawor J, Kocot-Zalewska J Tags: Mol Biol Rep Source Type: research
Influence of early rearing environment on water-borne cortisol and expression of stress-related genes in grass carp (Ctenopharyngodon idella).
Abstract Nowadays, lower post-release survivorship of hatchery-reared fish in natural aquatic bodies has attained great attention and research is in progress to determine the reasons for their higher mortality. It is assumed that hatchery rearing environments negatively affect the physiological stress response of the fish. Thus, understanding how rearing environments modulate this is important for the well-being of fish. Here, an attempt has been made to assess the influence of two early rearing environments, i.e., barren (BR), mimic the conventional hatchery rearing environment; without any substrate and enrichme...
Source: Molecular Biology Reports - June 10, 2020 Category: Molecular Biology Authors: Murtaza MUH, Zuberi A, Ahmad M, Amir I, Kamran M, Ahmad M Tags: Mol Biol Rep Source Type: research
Molecular inferences about the genus Hypostomus Lac épède, 1803 (Siluriformes: Loricariidae): a review.
Molecular inferences about the genus Hypostomus Lacépède, 1803 (Siluriformes: Loricariidae): a review. Mol Biol Rep. 2020 Jun 09;: Authors: Mezzomo P, Mielniczki-Pereira AA, Sausen TL, Reppold Marinho J, Cansian RL Abstract This review compiles and discusses the use of genetic markers applied in the study of the fish genus Hypostomus Lacépède, 1803 (Siluriformes: Loricariidae). The database comprises 51 peer-review articles that were published in the last 52 years (1968-2020) and that approach analysis based on different classes of genetic markers. The use of cyto...
Source: Molecular Biology Reports - June 9, 2020 Category: Molecular Biology Authors: Mezzomo P, Mielniczki-Pereira AA, Sausen TL, Reppold Marinho J, Cansian RL Tags: Mol Biol Rep Source Type: research
DNA repair genes hOGG1, XRCC1 and ERCC2 polymorphisms and their molecular mapping in breast cancer patients from India.
Abstract Identification of modifier genes predisposing to breast cancer (BC) phenotype remains a significant challenge and varies with ethnicity. The genetic variability observed in DNA repair genes may modulate the cell's ability to repair the damaged DNA and hence, evaluation of genetic variants in crucial DNA damage repair genes is of clinical importance. We performed the present study to evaluate the role of ERCC2-Lys751Gln, hOGG1-Ser326Cys, and XRCC1-Arg399Gln gene polymorphisms on the risk of BC development and its molecular profile in Indian women. Three non-synonymous variants (rs13181, rs1052133, and rs25...
Source: Molecular Biology Reports - June 9, 2020 Category: Molecular Biology Authors: Rajagopal T, Seshachalam A, Rathnam KK, Jothi A, Viswanathan S, Talluri S, Dunna NR Tags: Mol Biol Rep Source Type: research
Evaluation of multiple gene targeting in porcine embryos by the CRISPR/Cas9 system using electroporation.
Abstract The CRISPR/Cas9 system now allows for unprecedented possibilities of genome editing. However, there are some limitations, including achieving efficient one-step multiple genome targeting to save costs, time, and ensure high quality. In the present study, we investigated the efficiency of one-step multiple gene modification by electroporation in porcine zygotes using pooled guide RNAs (gRNAs) targeting CMAH, GHR, GGTA1, and PDX1. We first selected the best-performing gRNA from three different designs for each gene based on the effect on embryo development and mutation efficiency. The three gRNAs showed equ...
Source: Molecular Biology Reports - June 9, 2020 Category: Molecular Biology Authors: Hirata M, Wittayarat M, Namula Z, Le QA, Lin Q, Nguyen NT, Takebayashi K, Sato Y, Tanihara F, Otoi T Tags: Mol Biol Rep Source Type: research
Single X-ray irradiation modulates proteoglycan expression in brain tissue: investigation using mouse model.
Abstract Radiotherapy is an integral part of glioblastoma treatment affecting both cancer cells and tumour microenvironment, where proteoglycans (PGs) are key extracellular components. However, the molecular effects of radiotherapy on PGs expression and functional activity in brain tissue are poorly understood. Here, we aimed to study the short-term effects of X-ray irradiation on PGs expression in normal brain tissue in mouse model in vivo. Two-month-old male CBL/6Bl mice (n = 54) were used in this study, animals' brains were irradiated using either research synchrotron VEPP-4 or clinical linear acc...
Source: Molecular Biology Reports - June 8, 2020 Category: Molecular Biology Authors: Politko MO, Prokaeva AI, Pashkovskaya OA, Kuper KE, Zheravin AA, Kliver EE, Tsidulko AY, Aidagulova SV, Grigorieva EV Tags: Mol Biol Rep Source Type: research
The role of exosomes in stroke.
Abstract Stroke is induced by a partial disruption of cerebral blood flow to the brain and is related to high morbidity and mortality. In the central nervous system, exosomes have been proven to exert neuroprotective effects, reducing brain damage following a stroke. This review was performed by searching the relevant articles in the SCIENCEDIRECT, PUBMED, and Web of Science databases from respective inception to November 2018. We review the relationship between exosomes and angiogenesis, neurogenesis, antiapoptosis, autophagy, and the blood-brain barrier in stroke. Moreover, exosomes are found to be a promising t...
Source: Molecular Biology Reports - June 8, 2020 Category: Molecular Biology Authors: Wang MM, Feng YS, Tan ZX, Xing Y, Dong F, Zhang F Tags: Mol Biol Rep Source Type: research
Insights in the biology of extremely low-frequency magnetic fields exposure on human health.
Abstract The extremely low-frequency magnetic fields (ELF-EMF) are generated by electrical devices and power systems (1 to 300 Hz). In recent decades, exposure to ELF-EMF has emerged potential concerns on public health. Here, we discuss recent progress in the understanding of ELF-EMF biology with a focus on mechanisms of ELF-EMF-mediated disease and summarize the results of more recent experimental and epidemiological studies of ELF-EMF exposure effects on cancer, neurological, cardiovascular, and reproductive disorders. Current views on genomic instability effects, as well as scientific evidence about ELF-EM...
Source: Molecular Biology Reports - June 8, 2020 Category: Molecular Biology Authors: Karimi A, Ghadiri Moghaddam F, Valipour M Tags: Mol Biol Rep Source Type: research
The complete chloroplast genome and characteristics analysis of Callistemon rigidus R.Br.
This study demonstrates the data collected from the assembly and annotation of the chloroplast (cp) genome of Callistemon rigidus R.Br., followed by furthers comparative analysis with the cp genomes of closely related species. C. rigidus R.Br. showed a cp genome in the size of 158, 961 bp long with 36.78% GC content, among which a pair of inverted repeats (IRs) of 26, 671 bp separated a large single-copy (LSC) region of 87, 162 bp and a small single-copy (SSC) region of 18, 457 bp. Altogether 131 genes were hosted, including 37 transfer RNAs, 8 ribosomal RNAs, and 86 protein-coding genes. 284 simple seq...
Source: Molecular Biology Reports - June 8, 2020 Category: Molecular Biology Authors: Liu F, Movahedi A, Yang W, Xu L, Xie J, Zhang Y Tags: Mol Biol Rep Source Type: research
SMAD2 rs4940086 heterozygosity increases the risk of cervical cancer development among the women in Bangladesh.
In this study, we demonstrate the impact of single nucleotide polymorphisms (SNPs) of SMAD2 (rs4940086 and rs8085335) on cervical cancer risk development in Bangladeshi population. 132 cervical cancer patients and 98 control volunteers were enrolled in the study and genotyped utilizing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The association between cervical cancer susceptibility and the chosen SNPs were evaluated through multiple logistic regression. SMAD2 rs4940086 heterozygous genotype (T/C) was associated with a 3.89 times higher risk of cervical cancer development (P&thinsp...
Source: Molecular Biology Reports - June 7, 2020 Category: Molecular Biology Authors: Haque PS, Apu MNH, Nahid NA, Islam F, Islam MR, Hasnat A, Islam MS Tags: Mol Biol Rep Source Type: research
Synthetic biology in various cellular and molecular fields: applications, limitations, and perspective.
Abstract Synthetic biology breakthroughs have facilitated genetic circuit engineering to program cells through novel biological functions, dynamic gene expressions, as well as logic controls. SynBio can also participate in the rapid development of new treatments required for the human lifestyle. Moreover, these technologies are applied in the development of innovative therapeutic, diagnostic, as well as discovery-related methods within a wide range of cellular and molecular applications. In the present review study, SynBio applications in various cellular and molecular fields such as novel strategies for cancer th...
Source: Molecular Biology Reports - June 7, 2020 Category: Molecular Biology Authors: Safaei M, Mobini GR, Abiri A, Shojaeian A Tags: Mol Biol Rep Source Type: research
From air pollution to cardiovascular diseases: the emerging role of epigenetics.
From air pollution to cardiovascular diseases: the emerging role of epigenetics. Mol Biol Rep. 2020 Jun 06;: Authors: Micheu MM, Birsan MV, Szép R, Keresztesi Á, Nita IA Abstract The association between air pollution and a wide-ranging spectrum of acute and chronic disorders-including cardiovascular diseases-is widely acknowledged. Exposure to airborne pollutants triggers harmful mechanisms such as oxidative stress and systemic inflammation, which lead to increased incidence of myocardial infarction, arterial hypertension, stroke, and heart failure. Sustained efforts have ...
Source: Molecular Biology Reports - June 6, 2020 Category: Molecular Biology Authors: Micheu MM, Birsan MV, Szép R, Keresztesi Á, Nita IA Tags: Mol Biol Rep Source Type: research
Polymorphisms in GSTT1 and GSTM1 genes as possible risk factors for susceptibility to breast cancer development and their influence in chemotherapy response: a systematic review.
This study aimed to systematically review and investigate the existence of a possible correlation between the presence/absence of these genetic variants and the development of BC and their influence in chemotherapy response. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol was used, and the searches were performed in the portal of the Virtual Health Library (VHL) and the PubMed, resulting in 21 articles. It is clear that most studies revealed a risk association between the deletion of GSTM1 and/or GSTT1 and the development and/or prognosis of BC.Moreover, it should be noted that thes...
Source: Molecular Biology Reports - June 3, 2020 Category: Molecular Biology Authors: Pacholak LM, Amarante MK, Guembarovski RL, Watanabe MAE, Panis C Tags: Mol Biol Rep Source Type: research
The critical role of the interplays of EphrinB2/EphB4 and VEGF in the induction of angiogenesis.
Abstract The significant role of VEGF (vascular endothelial growth factor) as an angiogenesis inducer is well recognized. Besides VEGF, EphrinB2/EphB4 also plays essential roles in vascular development and postnatal angiogenesis. Compared with classical proangiogenic factors, not only does EphrinB2/EphB4 promote sprouting of new vessels, it is also involved in the vessel maturation. Given their involvement in many physiologic and pathological conditions, EphB4 and EphrinB2 are increasingly recognized as attractive therapeutic targets for angiogenesis-related diseases through modulating their expression and functio...
Source: Molecular Biology Reports - June 2, 2020 Category: Molecular Biology Authors: Du E, Li X, He S, Li X, He S Tags: Mol Biol Rep Source Type: research
Raspberry leaf blotch emaravirus in Bosnia and Herzegovina: population structure and systemic movement.
is IE Abstract Raspberry leaf blotch virus (RLBV) is the putative agent of the homonymous disease and even though Bosnia and Herzegovina is a major producer worldwide there is no report of the virus presence in the country. We studied the virus population structure and assessed its ability to move systemically. RLBV is widespread in production areas and has a homogeneous population structure; leading to the hypothesis that the primary mode of dissemination is propagation material. The ability of the virus to move systemically eliminates propagation of root cuttings as a viable option to obtain RLBV-free plants, le...
Source: Molecular Biology Reports - June 2, 2020 Category: Molecular Biology Authors: Delić D, Radulović M, Vakić M, A Sunulahpašić, Villamor DEV, Tzanetakis IE Tags: Mol Biol Rep Source Type: research
Transcriptome analysis of heat stress response genes in potato leaves.
In this study, two cDNA libraries from normal potato leaves (20 °C day/18 °C night) and potato leaves with 3 days of heat treatment (35 °C day/28 °C night) were constructed respectively. Totally, 1420 differentially expressed genes (DEGs) were identified. The expression patterns of 12 randomly selected genes detected using droplet digital PCR agreed with the sequencing data. Gene ontology analysis showed that these DEGs were clustered into 49 different GO types, reflecting the functional diversity of the heat stress response genes. The results of KEGG pathway enrichment sh...
Source: Molecular Biology Reports - June 1, 2020 Category: Molecular Biology Authors: Tang R, Gupta SK, Niu S, Li XQ, Yang Q, Chen G, Zhu W, Haroon M Tags: Mol Biol Rep Source Type: research