Apoptotic mechanisms of myricitrin isolated from Madhuca longifolia leaves in HL-60 leukemia cells
Mol Biol Rep. 2021 Jun 22. doi: 10.1007/s11033-021-06500-z. Online ahead of print.ABSTRACTMyricitrin, a naturally occurring flavonoid in Madhuca longifolia, possesses several medicinal properties. Even though our earlier work revealed its role against the proliferation of acute myelogenous leukemia cells (HL-60), its molecular mechanisms have not yet been revealed. The current study aims to explore the molecular mechanisms of myricitrin (isolated from an ethnomedicinal drug Madhuca longifolia) to induce apoptosis in HL-60 cells. Treatment with IC-50 dose of myricitrin (353 µM) caused cellular shrinkage and cell wall ...
Source: Molecular Biology Reports - June 22, 2021 Category: Molecular Biology Authors: Monaj Kumar Sarkar Amrita Kar Adithyan Jayaraman Karthi Shanmugam Vellingiri Vadivel Santanu Kar Mahapatra Source Type: research

Apoptotic mechanisms of myricitrin isolated from Madhuca longifolia leaves in HL-60 leukemia cells
Mol Biol Rep. 2021 Jun 22. doi: 10.1007/s11033-021-06500-z. Online ahead of print.ABSTRACTMyricitrin, a naturally occurring flavonoid in Madhuca longifolia, possesses several medicinal properties. Even though our earlier work revealed its role against the proliferation of acute myelogenous leukemia cells (HL-60), its molecular mechanisms have not yet been revealed. The current study aims to explore the molecular mechanisms of myricitrin (isolated from an ethnomedicinal drug Madhuca longifolia) to induce apoptosis in HL-60 cells. Treatment with IC-50 dose of myricitrin (353 µM) caused cellular shrinkage and cell wall ...
Source: Molecular Biology Reports - June 22, 2021 Category: Molecular Biology Authors: Monaj Kumar Sarkar Amrita Kar Adithyan Jayaraman Karthi Shanmugam Vellingiri Vadivel Santanu Kar Mahapatra Source Type: research

Low oxygen tension potentiates proliferation and stemness but not multilineage differentiation of caprine male germline stem cells
Mol Biol Rep. 2021 Jun 20. doi: 10.1007/s11033-021-06501-y. Online ahead of print.ABSTRACTThe milieu of male germline stem cells (mGSCs) is characterized as a low-oxygen (O2) environment, whereas, their in-vitro expansion is typically performed under normoxia (20-21% O2). The comparative information about the effects of low and normal O2 levels on the growth and differentiation of caprine mGSCs (cmGSCs) is lacking. Thus, we aimed to investigate the functional and multilineage differentiation characteristics of enriched cmGSCs, when grown under hypoxia and normoxia. After enrichment of cmGSCs through multiple methods (diffe...
Source: Molecular Biology Reports - June 20, 2021 Category: Molecular Biology Authors: Shiva Pratap Singh Suresh Dinkar Kharche Manisha Pathak Ravi Ranjan Yogesh Kumar Soni Manoj Kumar Singh Ramasamy Pourouchottamane Manmohan Singh Chauhan Source Type: research

Exon-1 skipping and intron-1 retaining by alternative splicing of the c-KIT gene encodes a novel splice variant in the skin of Merino sheep (Ovis aries)
Mol Biol Rep. 2021 Jun 20. doi: 10.1007/s11033-021-06486-8. Online ahead of print.ABSTRACTc-KIT, a type III receptor protein tyrosine kinase, plays an essential role in melanocyte development, migration, and survival. Mutations within the c-KIT gene are previously shown to cause the white coat color phenotypes in pigs, mice, goats, and humans. However, up so far, the splicing isoform(s), genomic architecture of c-KIT have not been characterized well in merino sheep. Reverse transcriptase (RT)-PCR analysis with molecular prediction identified two basic splice variants: Transcript Variant-1, 2 for 12 bp insertion coding sequ...
Source: Molecular Biology Reports - June 20, 2021 Category: Molecular Biology Authors: Siva Arumugam Saravanaperumal Stefano Pallotti Dario Pediconi Carlo Renieri Antonietta La Terza Source Type: research

Genetic relationships and diversity analysis in Turkish laurel (Laurus nobilis L.) germplasm using ISSR and SCoT markers
In this study, molecular characterization, and genetic relationships of 94 Turkish laurel genotypes were determined by ISSR and SCoT markers. The experiment was conducted with 16 ISSR and 10 SCoT markers. While 348 of 373 bands were polymorphic with a 93.3% polymorphism rate, Nei's genetic distances ranged between 0.17 and 0.70 with 0.39 mean in ISSR. In SCoT, 175 of 227 bands were polymorphic with 77.1% polymorphism rate, and Nei's genetic distances varied between 0.12 and 0.51. Sufficient genetic diversity determined with diversity parameters consisting of the average Shannon's information index (ISSR: 0.46, SCoT:0.35), ...
Source: Molecular Biology Reports - June 20, 2021 Category: Molecular Biology Authors: Abdurrahim Yilmaz Vahdettin Ciftci Source Type: research

Low oxygen tension potentiates proliferation and stemness but not multilineage differentiation of caprine male germline stem cells
Mol Biol Rep. 2021 Jun 20. doi: 10.1007/s11033-021-06501-y. Online ahead of print.ABSTRACTThe milieu of male germline stem cells (mGSCs) is characterized as a low-oxygen (O2) environment, whereas, their in-vitro expansion is typically performed under normoxia (20-21% O2). The comparative information about the effects of low and normal O2 levels on the growth and differentiation of caprine mGSCs (cmGSCs) is lacking. Thus, we aimed to investigate the functional and multilineage differentiation characteristics of enriched cmGSCs, when grown under hypoxia and normoxia. After enrichment of cmGSCs through multiple methods (diffe...
Source: Molecular Biology Reports - June 20, 2021 Category: Molecular Biology Authors: Shiva Pratap Singh Suresh Dinkar Kharche Manisha Pathak Ravi Ranjan Yogesh Kumar Soni Manoj Kumar Singh Ramasamy Pourouchottamane Manmohan Singh Chauhan Source Type: research

Exon-1 skipping and intron-1 retaining by alternative splicing of the c-KIT gene encodes a novel splice variant in the skin of Merino sheep (Ovis aries)
Mol Biol Rep. 2021 Jun 20. doi: 10.1007/s11033-021-06486-8. Online ahead of print.ABSTRACTc-KIT, a type III receptor protein tyrosine kinase, plays an essential role in melanocyte development, migration, and survival. Mutations within the c-KIT gene are previously shown to cause the white coat color phenotypes in pigs, mice, goats, and humans. However, up so far, the splicing isoform(s), genomic architecture of c-KIT have not been characterized well in merino sheep. Reverse transcriptase (RT)-PCR analysis with molecular prediction identified two basic splice variants: Transcript Variant-1, 2 for 12 bp insertion coding sequ...
Source: Molecular Biology Reports - June 20, 2021 Category: Molecular Biology Authors: Siva Arumugam Saravanaperumal Stefano Pallotti Dario Pediconi Carlo Renieri Antonietta La Terza Source Type: research

Genetic relationships and diversity analysis in Turkish laurel (Laurus nobilis L.) germplasm using ISSR and SCoT markers
In this study, molecular characterization, and genetic relationships of 94 Turkish laurel genotypes were determined by ISSR and SCoT markers. The experiment was conducted with 16 ISSR and 10 SCoT markers. While 348 of 373 bands were polymorphic with a 93.3% polymorphism rate, Nei's genetic distances ranged between 0.17 and 0.70 with 0.39 mean in ISSR. In SCoT, 175 of 227 bands were polymorphic with 77.1% polymorphism rate, and Nei's genetic distances varied between 0.12 and 0.51. Sufficient genetic diversity determined with diversity parameters consisting of the average Shannon's information index (ISSR: 0.46, SCoT:0.35), ...
Source: Molecular Biology Reports - June 20, 2021 Category: Molecular Biology Authors: Abdurrahim Yilmaz Vahdettin Ciftci Source Type: research

Identifying shark species responsible for fisheries depredation off Southeast Queensland, Australia
Mol Biol Rep. 2021 Jun 18. doi: 10.1007/s11033-021-06460-4. Online ahead of print.ABSTRACTAnecdotal reports from fishers in Southeast Queensland, Australia suggest that shark depredation is a significant issue, however little is known about which species are responsible for depredating catches. This research aimed to identify depredating species in Southeast Queensland line based fisheries, by undertaking a genetic analysis of depredated samples collected by commercial, charter and recreational fishers. The genetic analysis successfully identified ten depredating sharks, all from the genus Carcharhinus (19.2% success). The...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Jaeden L Vardon Samuel M Williams Daniel J Bucher Jess A T Morgan Source Type: research

Effects of mesenchymal stem cell-derived exosomes on oxidative stress responses in skin cells
This study aimed to verify the preventive or therapeutic effects of mesenchymal stem cell-derived exosomes (MSC-Ex) on the production of ROS induced by oxidative stress in human skin fibroblasts and clarify the mechanisms that promote cellular senescence. In a system where H2O2 was applied to skin fibroblasts, we assessed the effects of the application of MSC-Ex before and after oxidative stress and measured the fluctuations in several signaling molecules involved in subsequent intracellular stress responses. Exosomes were isolated from MSCs (MSC-Ex) and normal human dermal fibroblasts (NHDFs, NHDF-Ex) before and after exp...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Takaaki Matsuoka Keita Takanashi Katsuaki Dan Kenichi Yamamoto Koji Tomobe Tatsuo Shinozuka Source Type: research

ELF5 inhibits the proliferation and invasion of breast cancer cells by regulating CD24
In this study, we examined the effect of ELF5 on the human breast cancer cell lines MCF-7 and T47D and confirmed that ELF5 can inhibit cell proliferation, migration and invasion. In further research, the relationship between ELF5 and CD24 was characterized in breast cancer cells. We found that CD24 was a target gene of ELF5 through chromatin immunoprecipitation (ChIP) -Sequence assays, and proved that ELF5 could bind to the ETS cis-element on the proximal promoter of the CD24 gene and regulate the expression of CD24. Moreover, overexpression of ELF5 in MCF-7 cells significantly increased both the mRNA and protein levels of...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Xinjian Qu Qianqian Li Simei Tu Xiaocheng Yang Wen Wen Source Type: research

Serum microRNA-33 levels in pre-diabetic and diabetic patients
Mol Biol Rep. 2021 Jun 19. doi: 10.1007/s11033-021-06425-7. Online ahead of print.ABSTRACTDiabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia and abnormal insulin secretion. MicroRNAs are small, non-coding RNAs that are able to affect cell biological functions and act as biomarkers for some diseases such as DM. In current study, we measured serum miR-33 in three groups (n = 15) as follows; non-diabetic control, pre-diabetic, and DM patients. Real-time PCR method was used to quantify miR-33 expression. miR-33 expression was significantly increased in pre-diabetic subjects compared to other two groups (p
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Beydolah Shahouzehi Mostafa Eghbalian Hossein Fallah Soheil Aminizadeh Yaser Masoumi-Ardakani Source Type: research

A novel BRCA1 duplication and new insights on the spectrum and frequency of germline large genomic rearrangements in BRCA1/BRCA2
In this study, we performed a next-generation sequencing panel and MLPA on 1484 patients to explain the importance of recurrent germline duplications/deletions of BRCA1-2 and their clinical results and determine how often BRCA gene LGRs were seen in people suspected of hereditary breast and ovarian cancer syndrome. The large genomic rearrangements (LGRs) frequency was approximately 1% (14/1484). All 14 mutations were heterozygous and detected in patients with breast cancer. BRCA1 mutations were more predominant (n = 8, 57.1%) than BRCA2 mutations (6, 42.9%). The most common recurrent mutations were BRCA2 exon three and BRC...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Ibrahim Sahin Hanife Saat Source Type: research

Platelet activation and antimicrobial activity of L-PRF: a preliminary study
Mol Biol Rep. 2021 Jun 19. doi: 10.1007/s11033-021-06487-7. Online ahead of print.ABSTRACTLeukocyte and platelet rich fibrin (L-PRF) is one of the platelet concentrates used to support regeneration and healing process. Many studies showed possible immunological and antibacterial properties of L-PRF. We perform an in vitro study to analyze the effect of L-PRF on platelet activation, platelet-leukocytes interactions and antimicrobial activity, important components in the healing process. Molecular biomarkers related with platelet activation and platelet-leukocyte interactions were analyzed by means of flow cytometry when L-P...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Ant ónio Melo-Ferraz Cristina Coelho Paulo Miller Maria Bego ña Criado Maria C éu Monteiro Source Type: research

Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis
Mol Biol Rep. 2021 Jun 19. doi: 10.1007/s11033-021-06494-8. Online ahead of print.ABSTRACTBovine mastitis, an inflammatory disease of the mammary gland, is classified as subclinical or clinical. Circulating neutrophils are recruited to the udder to combat infection. We compared the transcriptomic profiles in circulating leukocytes between healthy cows and those with naturally occurring subclinical or clinical mastitis. Holstein Friesian dairy cows from six farms in EU countries were recruited. Based on milk somatic cell count and clinical records, cows were classified as healthy (n = 147), subclinically (n = 45) or clinica...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Zhangrui Cheng Laura Buggiotti Mazdak Salavati Cinzia Marchitelli Sergio Palma-Vera Alistair Wylie Haruko Takeda Lijing Tang Mark A Crowe D Claire Wathes GplusE consortium Source Type: research

Identifying shark species responsible for fisheries depredation off Southeast Queensland, Australia
Mol Biol Rep. 2021 Jun 18. doi: 10.1007/s11033-021-06460-4. Online ahead of print.ABSTRACTAnecdotal reports from fishers in Southeast Queensland, Australia suggest that shark depredation is a significant issue, however little is known about which species are responsible for depredating catches. This research aimed to identify depredating species in Southeast Queensland line based fisheries, by undertaking a genetic analysis of depredated samples collected by commercial, charter and recreational fishers. The genetic analysis successfully identified ten depredating sharks, all from the genus Carcharhinus (19.2% success). The...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Jaeden L Vardon Samuel M Williams Daniel J Bucher Jess A T Morgan Source Type: research

Effects of mesenchymal stem cell-derived exosomes on oxidative stress responses in skin cells
This study aimed to verify the preventive or therapeutic effects of mesenchymal stem cell-derived exosomes (MSC-Ex) on the production of ROS induced by oxidative stress in human skin fibroblasts and clarify the mechanisms that promote cellular senescence. In a system where H2O2 was applied to skin fibroblasts, we assessed the effects of the application of MSC-Ex before and after oxidative stress and measured the fluctuations in several signaling molecules involved in subsequent intracellular stress responses. Exosomes were isolated from MSCs (MSC-Ex) and normal human dermal fibroblasts (NHDFs, NHDF-Ex) before and after exp...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Takaaki Matsuoka Keita Takanashi Katsuaki Dan Kenichi Yamamoto Koji Tomobe Tatsuo Shinozuka Source Type: research

ELF5 inhibits the proliferation and invasion of breast cancer cells by regulating CD24
In this study, we examined the effect of ELF5 on the human breast cancer cell lines MCF-7 and T47D and confirmed that ELF5 can inhibit cell proliferation, migration and invasion. In further research, the relationship between ELF5 and CD24 was characterized in breast cancer cells. We found that CD24 was a target gene of ELF5 through chromatin immunoprecipitation (ChIP) -Sequence assays, and proved that ELF5 could bind to the ETS cis-element on the proximal promoter of the CD24 gene and regulate the expression of CD24. Moreover, overexpression of ELF5 in MCF-7 cells significantly increased both the mRNA and protein levels of...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Xinjian Qu Qianqian Li Simei Tu Xiaocheng Yang Wen Wen Source Type: research

Serum microRNA-33 levels in pre-diabetic and diabetic patients
Mol Biol Rep. 2021 Jun 19. doi: 10.1007/s11033-021-06425-7. Online ahead of print.ABSTRACTDiabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia and abnormal insulin secretion. MicroRNAs are small, non-coding RNAs that are able to affect cell biological functions and act as biomarkers for some diseases such as DM. In current study, we measured serum miR-33 in three groups (n = 15) as follows; non-diabetic control, pre-diabetic, and DM patients. Real-time PCR method was used to quantify miR-33 expression. miR-33 expression was significantly increased in pre-diabetic subjects compared to other two groups (p
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Beydolah Shahouzehi Mostafa Eghbalian Hossein Fallah Soheil Aminizadeh Yaser Masoumi-Ardakani Source Type: research

A novel BRCA1 duplication and new insights on the spectrum and frequency of germline large genomic rearrangements in BRCA1/BRCA2
In this study, we performed a next-generation sequencing panel and MLPA on 1484 patients to explain the importance of recurrent germline duplications/deletions of BRCA1-2 and their clinical results and determine how often BRCA gene LGRs were seen in people suspected of hereditary breast and ovarian cancer syndrome. The large genomic rearrangements (LGRs) frequency was approximately 1% (14/1484). All 14 mutations were heterozygous and detected in patients with breast cancer. BRCA1 mutations were more predominant (n = 8, 57.1%) than BRCA2 mutations (6, 42.9%). The most common recurrent mutations were BRCA2 exon three and BRC...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Ibrahim Sahin Hanife Saat Source Type: research

Platelet activation and antimicrobial activity of L-PRF: a preliminary study
Mol Biol Rep. 2021 Jun 19. doi: 10.1007/s11033-021-06487-7. Online ahead of print.ABSTRACTLeukocyte and platelet rich fibrin (L-PRF) is one of the platelet concentrates used to support regeneration and healing process. Many studies showed possible immunological and antibacterial properties of L-PRF. We perform an in vitro study to analyze the effect of L-PRF on platelet activation, platelet-leukocytes interactions and antimicrobial activity, important components in the healing process. Molecular biomarkers related with platelet activation and platelet-leukocyte interactions were analyzed by means of flow cytometry when L-P...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Ant ónio Melo-Ferraz Cristina Coelho Paulo Miller Maria Bego ña Criado Maria C éu Monteiro Source Type: research

Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis
Mol Biol Rep. 2021 Jun 19. doi: 10.1007/s11033-021-06494-8. Online ahead of print.ABSTRACTBovine mastitis, an inflammatory disease of the mammary gland, is classified as subclinical or clinical. Circulating neutrophils are recruited to the udder to combat infection. We compared the transcriptomic profiles in circulating leukocytes between healthy cows and those with naturally occurring subclinical or clinical mastitis. Holstein Friesian dairy cows from six farms in EU countries were recruited. Based on milk somatic cell count and clinical records, cows were classified as healthy (n = 147), subclinically (n = 45) or clinica...
Source: Molecular Biology Reports - June 19, 2021 Category: Molecular Biology Authors: Zhangrui Cheng Laura Buggiotti Mazdak Salavati Cinzia Marchitelli Sergio Palma-Vera Alistair Wylie Haruko Takeda Lijing Tang Mark A Crowe D Claire Wathes GplusE consortium Source Type: research

CRISPR/Cas based gene editing: marking a new era in medical science
Mol Biol Rep. 2021 Jun 18. doi: 10.1007/s11033-021-06479-7. Online ahead of print.ABSTRACTCRISPR/Cas9 system, a bacterial adaptive immune system developed into a genome editing technology, has emerged as a powerful tool revolutionising genome engineering in all branches of biological science including agriculture, research and medicine. Rapid evolution of CRISPR/Cas9 system from the generation of double strand breaks to more advanced applications on gene regulation has made the wide-spread use of this technology possible. Medical science has benefited greatly from CRISPR/Cas9; being both a versatile and economical tool, it...
Source: Molecular Biology Reports - June 18, 2021 Category: Molecular Biology Authors: Kirti Prasad Anila George Nithin Sam Ravi Kumarasamypet M Mohankumar Source Type: research

Studies on the role of alpha 7 nicotinic acetylcholine receptors in K562 cell proliferation and signaling
This study is primarily to determine the role of nicotinic agonists and antagonists in cell proliferation, α7 nicotinic acetylcholine receptor expression, and calcium release. The aim of this study, which is a continuation and an important part of our previous studies on the cholinergic system, has contributed to the literature on the human erythroleukemia cell signaling mechanism. Cell viability was evaluated by the trypan blue exclusion test and Bromodeoxyuridine/5-Bromo-2'-deoxyuridine (BrdU) labeling. Acetylcholine, nicotinic alpha 7 receptor antagonist methyllycaconitine citrate, and cholinergic antagonist atrop...
Source: Molecular Biology Reports - June 18, 2021 Category: Molecular Biology Authors: G özde Önder Narin Banu Ayd ın H ülya Cabadak Source Type: research

Lens regeneration: scientific discoveries and clinical possibilities
This article will briefly summarize the regulatory mechanisms of lens development, describe the recent progress of lens regeneration, explore the key molecular signaling pathways, and, more importantly, discuss the prospects and challenges of their clinical applications to provide reference for clinical transformations.PMID:34143397 | DOI:10.1007/s11033-021-06489-5 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 18, 2021 Category: Molecular Biology Authors: Yuzhou Gu Ke Yao Qiuli Fu Source Type: research

CRISPR/Cas based gene editing: marking a new era in medical science
Mol Biol Rep. 2021 Jun 18. doi: 10.1007/s11033-021-06479-7. Online ahead of print.ABSTRACTCRISPR/Cas9 system, a bacterial adaptive immune system developed into a genome editing technology, has emerged as a powerful tool revolutionising genome engineering in all branches of biological science including agriculture, research and medicine. Rapid evolution of CRISPR/Cas9 system from the generation of double strand breaks to more advanced applications on gene regulation has made the wide-spread use of this technology possible. Medical science has benefited greatly from CRISPR/Cas9; being both a versatile and economical tool, it...
Source: Molecular Biology Reports - June 18, 2021 Category: Molecular Biology Authors: Kirti Prasad Anila George Nithin Sam Ravi Kumarasamypet M Mohankumar Source Type: research

Studies on the role of alpha 7 nicotinic acetylcholine receptors in K562 cell proliferation and signaling
This study is primarily to determine the role of nicotinic agonists and antagonists in cell proliferation, α7 nicotinic acetylcholine receptor expression, and calcium release. The aim of this study, which is a continuation and an important part of our previous studies on the cholinergic system, has contributed to the literature on the human erythroleukemia cell signaling mechanism. Cell viability was evaluated by the trypan blue exclusion test and Bromodeoxyuridine/5-Bromo-2'-deoxyuridine (BrdU) labeling. Acetylcholine, nicotinic alpha 7 receptor antagonist methyllycaconitine citrate, and cholinergic antagonist atrop...
Source: Molecular Biology Reports - June 18, 2021 Category: Molecular Biology Authors: G özde Önder Narin Banu Ayd ın H ülya Cabadak Source Type: research

Lens regeneration: scientific discoveries and clinical possibilities
This article will briefly summarize the regulatory mechanisms of lens development, describe the recent progress of lens regeneration, explore the key molecular signaling pathways, and, more importantly, discuss the prospects and challenges of their clinical applications to provide reference for clinical transformations.PMID:34143397 | DOI:10.1007/s11033-021-06489-5 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 18, 2021 Category: Molecular Biology Authors: Yuzhou Gu Ke Yao Qiuli Fu Source Type: research

The cytotoxic and apoptotic effects of beta-blockers with different selectivity on cancerous and healthy lung cell lines
Mol Biol Rep. 2021 Jun 16. doi: 10.1007/s11033-021-06409-7. Online ahead of print.ABSTRACTβ-blockers having specific affinities to β-adrenergic receptors are routinely used to treat cardiovascular problems. Additionally, it has been demonstrated that these drugs can be effective in treating apoptosis-related diseases. The current study was conducted to investigate the cytotoxic and apoptotic effects of β-1 selective esmolol, β-2 selective ICI-118,551, and non-selective nadolol blockers on the cancerous and healthy lung cells. MTT test was used to evaluate cytotoxicity. Apoptotic actions were examined by...
Source: Molecular Biology Reports - June 17, 2021 Category: Molecular Biology Authors: Berna Kavakc ıoğlu Yardımcı Fatime Geyikoglu Ferhunde Aysin Kubra Koc Nihal Simsek Ozek Vural K üçükatay Source Type: research

Serum fatty acid binding protein 5 (FABP5) as a potential biomarker of inflammation in psoriasis
Mol Biol Rep. 2021 Jun 15. doi: 10.1007/s11033-021-06461-3. Online ahead of print.ABSTRACTFatty acid binding protein 5 (FABP5) is elevated in psoriatic keratinocytes and could be involved in systemic metabolic disturbances in psoriasis. The aim of the study was to evaluate serum FABP5 in obese and non-obese psoriatic patients, to assess the relationship between FABP5 and the duration, severity of the disease, inflammatory and metabolic markers and influence of treatment with narrowband-ultraviolet B (NB-UVB). Seventy-four patients (30 treated with NB-UVB) with psoriasis were enrolled in the study. The serum concentrations ...
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: Dorota Koz łowska Hanna My śliwiec Ewa Harasim-Symbor Anna Justyna Milewska Adrian Chabowski Iwona Flisiak Source Type: research

mTOR-Rictor-EGFR axis in oncogenesis and diagnosis of glioblastoma multiforme
Mol Biol Rep. 2021 Jun 16. doi: 10.1007/s11033-021-06462-2. Online ahead of print.ABSTRACTGlioblastoma multiforme (GBM) is one of the aggressive brain cancers with patients having less survival period upto 12-15 months. Mammalian target of rapamycin (mTOR) is a serine/threonine kinase, belongs to the phosphatidylinositol 3-kinases (PI3K) pathway and is involved in various cellular processes of cancer cells. Cancer metabolism is regulated by mTOR and its components. mTOR forms two complexes as mTORC1 and mTORC2. Studies have identified the key component of the mTORC2 complex, Rapamycin-insensitive companion of mammalian tar...
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: M Janaki Ramaiah K Rohil Kumar Source Type: research

GLIS1, a potential candidate gene affect fat deposition in sheep tail
This study gives a new insight into the genetic basis of species-specific traits of sheep tails.PMID:34132943 | DOI:10.1007/s11033-021-06468-w (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: Rongsong Luo Xiaoran Zhang Likai Wang Li Zhang Guangpeng Li Zhong Zheng Source Type: research

Evaluation of circulating microRNAs-211 and 25 as diagnostic biomarkers of colorectal cancer
Mol Biol Rep. 2021 Jun 16. doi: 10.1007/s11033-021-06493-9. Online ahead of print.ABSTRACTColorectal cancer is one of the most prevalent and deadly cancers worldwide. MicroRNAs are short single stranded non-coding RNAs that play important roles in carcinogenesis, tumor growth and tumor survival. Circulating microRNAs are increasingly becoming efficient and important biomarkers for several types of cancers. Herein, we aim to evaluate the diagnostic potentials of plasma microRNA-211 and microRNA-25 in colorectal cancer patients. Forty-four patients diagnosed with colorectal cancer and 40 healthy controls were recruited for t...
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: Eman Radwan Asmaa S Shaltout Shima Gafar Mansor Engy A Shafik Wael A Abbas Mahmoud Refaat Shehata Maha Ali Source Type: research

Correlation between expression levels of lncRNA FER1L4 and RB1 in patients with colorectal cancer
Mol Biol Rep. 2021 Jun 16. doi: 10.1007/s11033-021-06488-6. Online ahead of print.ABSTRACTColorectal cancer (CRC) is a major life-threatening malignancy. Studies demonstrated the lncRNA fer-1 like family member 4 (FER1L4) was downregulated in different cancers and its expression was positively correlated with the retinoblastoma 1 (RB1) mRNA in a competing endogenous RNAs network. We investigated expression levels of FER1L4 and RB1 in patients with colorectal cancer. 50 paired colorectal tumors and non-tumor marginal tissues, 30 paired adenomatous colorectal polyps (ACPs) and matched adjacent normal tissues were obtained fr...
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: Marjan Ostovarpour Mohammad Khalaj-Kondori Tayyebeh Ghasemi Source Type: research

Duplex-RT-PCR assay for the simultaneous detection and discrimination of Brome mosaic virus and Cocksfoot mottle virus in cereal plants
In conclusion, duplex-RT-PCR is a useful tool which can be implemented by phytosanitary services to rapid detection and differentiation of BMV and CfMV.PMID:34132946 | DOI:10.1007/s11033-021-06485-9 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: Katarzyna Trzmiel Source Type: research

Serum fatty acid binding protein 5 (FABP5) as a potential biomarker of inflammation in psoriasis
Mol Biol Rep. 2021 Jun 15. doi: 10.1007/s11033-021-06461-3. Online ahead of print.ABSTRACTFatty acid binding protein 5 (FABP5) is elevated in psoriatic keratinocytes and could be involved in systemic metabolic disturbances in psoriasis. The aim of the study was to evaluate serum FABP5 in obese and non-obese psoriatic patients, to assess the relationship between FABP5 and the duration, severity of the disease, inflammatory and metabolic markers and influence of treatment with narrowband-ultraviolet B (NB-UVB). Seventy-four patients (30 treated with NB-UVB) with psoriasis were enrolled in the study. The serum concentrations ...
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: Dorota Koz łowska Hanna My śliwiec Ewa Harasim-Symbor Anna Justyna Milewska Adrian Chabowski Iwona Flisiak Source Type: research

mTOR-Rictor-EGFR axis in oncogenesis and diagnosis of glioblastoma multiforme
Mol Biol Rep. 2021 Jun 16. doi: 10.1007/s11033-021-06462-2. Online ahead of print.ABSTRACTGlioblastoma multiforme (GBM) is one of the aggressive brain cancers with patients having less survival period upto 12-15 months. Mammalian target of rapamycin (mTOR) is a serine/threonine kinase, belongs to the phosphatidylinositol 3-kinases (PI3K) pathway and is involved in various cellular processes of cancer cells. Cancer metabolism is regulated by mTOR and its components. mTOR forms two complexes as mTORC1 and mTORC2. Studies have identified the key component of the mTORC2 complex, Rapamycin-insensitive companion of mammalian tar...
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: M Janaki Ramaiah K Rohil Kumar Source Type: research

GLIS1, a potential candidate gene affect fat deposition in sheep tail
This study gives a new insight into the genetic basis of species-specific traits of sheep tails.PMID:34132943 | DOI:10.1007/s11033-021-06468-w (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: Rongsong Luo Xiaoran Zhang Likai Wang Li Zhang Guangpeng Li Zhong Zheng Source Type: research

Evaluation of circulating microRNAs-211 and 25 as diagnostic biomarkers of colorectal cancer
Mol Biol Rep. 2021 Jun 16. doi: 10.1007/s11033-021-06493-9. Online ahead of print.ABSTRACTColorectal cancer is one of the most prevalent and deadly cancers worldwide. MicroRNAs are short single stranded non-coding RNAs that play important roles in carcinogenesis, tumor growth and tumor survival. Circulating microRNAs are increasingly becoming efficient and important biomarkers for several types of cancers. Herein, we aim to evaluate the diagnostic potentials of plasma microRNA-211 and microRNA-25 in colorectal cancer patients. Forty-four patients diagnosed with colorectal cancer and 40 healthy controls were recruited for t...
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: Eman Radwan Asmaa S Shaltout Shima Gafar Mansor Engy A Shafik Wael A Abbas Mahmoud Refaat Shehata Maha Ali Source Type: research

Correlation between expression levels of lncRNA FER1L4 and RB1 in patients with colorectal cancer
Mol Biol Rep. 2021 Jun 16. doi: 10.1007/s11033-021-06488-6. Online ahead of print.ABSTRACTColorectal cancer (CRC) is a major life-threatening malignancy. Studies demonstrated the lncRNA fer-1 like family member 4 (FER1L4) was downregulated in different cancers and its expression was positively correlated with the retinoblastoma 1 (RB1) mRNA in a competing endogenous RNAs network. We investigated expression levels of FER1L4 and RB1 in patients with colorectal cancer. 50 paired colorectal tumors and non-tumor marginal tissues, 30 paired adenomatous colorectal polyps (ACPs) and matched adjacent normal tissues were obtained fr...
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: Marjan Ostovarpour Mohammad Khalaj-Kondori Tayyebeh Ghasemi Source Type: research

Duplex-RT-PCR assay for the simultaneous detection and discrimination of Brome mosaic virus and Cocksfoot mottle virus in cereal plants
In conclusion, duplex-RT-PCR is a useful tool which can be implemented by phytosanitary services to rapid detection and differentiation of BMV and CfMV.PMID:34132946 | DOI:10.1007/s11033-021-06485-9 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - June 16, 2021 Category: Molecular Biology Authors: Katarzyna Trzmiel Source Type: research

Impacts of OrX and cAMP-insensitive Orco to the insect olfactory heteromer activity
Mol Biol Rep. 2021 Jun 11. doi: 10.1007/s11033-021-06480-0. Online ahead of print.ABSTRACTInsect odorant receptors (ORs) have been suggested to function as ligand-gated cation channels, with OrX/Orco heteromers combining ionotropic and metabotropic activity. The latter is mediated by different G proteins and results in Orco self-activation by cyclic nucleotide binding. In this contribution, we co-express the odor-specific subunits DmOr49b and DmOr59b with either wild-type Orco or an Orco-PKC mutant lacking cAMP activation heterologously in mammalian cells. We show that the characteristics of heteromers strongly depend on b...
Source: Molecular Biology Reports - June 15, 2021 Category: Molecular Biology Authors: Danila V Kolesov Violetta O Ivanova Elena L Sokolinskaya Liubov A Kost Pavel M Balaban Konstantin A Lukyanov Evgeny S Nikitin Alexey M Bogdanov Source Type: research

Elastic tissue disruption is a major pathogenic factor to human vascular disease
Mol Biol Rep. 2021 Jun 15. doi: 10.1007/s11033-021-06478-8. Online ahead of print.ABSTRACTElastic fibers are essential components of the arterial extracellular matrix. They consist of the protein elastin and an array of microfibrils that support the protein and connect it to the surrounding matrix. The elastin gene encodes tropoelastin, a protein that requires extensive cross-linking to become elastin. Tropoelastin is expressed throughout human life, but its expression levels decrease with age, suggesting that the potential to synthesize elastin persists during lifetime although declines with aging. The initial abnormality...
Source: Molecular Biology Reports - June 15, 2021 Category: Molecular Biology Authors: Mar ía M Adeva-Andany Luc ía Adeva-Contreras Carlos Fern ández-Fernández Manuel Gonz ález-Lucán Raquel Funcasta-Calder ón Source Type: research

Impacts of OrX and cAMP-insensitive Orco to the insect olfactory heteromer activity
Mol Biol Rep. 2021 Jun 11. doi: 10.1007/s11033-021-06480-0. Online ahead of print.ABSTRACTInsect odorant receptors (ORs) have been suggested to function as ligand-gated cation channels, with OrX/Orco heteromers combining ionotropic and metabotropic activity. The latter is mediated by different G proteins and results in Orco self-activation by cyclic nucleotide binding. In this contribution, we co-express the odor-specific subunits DmOr49b and DmOr59b with either wild-type Orco or an Orco-PKC mutant lacking cAMP activation heterologously in mammalian cells. We show that the characteristics of heteromers strongly depend on b...
Source: Molecular Biology Reports - June 15, 2021 Category: Molecular Biology Authors: Danila V Kolesov Violetta O Ivanova Elena L Sokolinskaya Liubov A Kost Pavel M Balaban Konstantin A Lukyanov Evgeny S Nikitin Alexey M Bogdanov Source Type: research

Elastic tissue disruption is a major pathogenic factor to human vascular disease
Mol Biol Rep. 2021 Jun 15. doi: 10.1007/s11033-021-06478-8. Online ahead of print.ABSTRACTElastic fibers are essential components of the arterial extracellular matrix. They consist of the protein elastin and an array of microfibrils that support the protein and connect it to the surrounding matrix. The elastin gene encodes tropoelastin, a protein that requires extensive cross-linking to become elastin. Tropoelastin is expressed throughout human life, but its expression levels decrease with age, suggesting that the potential to synthesize elastin persists during lifetime although declines with aging. The initial abnormality...
Source: Molecular Biology Reports - June 15, 2021 Category: Molecular Biology Authors: Mar ía M Adeva-Andany Luc ía Adeva-Contreras Carlos Fern ández-Fernández Manuel Gonz ález-Lucán Raquel Funcasta-Calder ón Source Type: research

Quisinostat mediated autophagy is associated with differentiation in neuroblastoma SK-N-SH cells
Mol Biol Rep. 2021 Jun 14. doi: 10.1007/s11033-021-06481-z. Online ahead of print.ABSTRACTNeuroblastoma (NB) is the most common childhood cancer that arises from the sympathetic nervous system. NB is characterized by poor prognosis. One of the strategies to control NB is activating the differentiation process in undifferentiated NB cells. Many differentiating agents including 13-cis-retinoic acid (RA) led to disappointing results. In the current study, we investigated the effect of Quisinostat/JNJ-26481585(JNJ) on NB SK-N-SH cells differentiation. The SK-N-SH cell differentiation was observed by morphology and neurite leng...
Source: Molecular Biology Reports - June 14, 2021 Category: Molecular Biology Authors: Vamsi Krishna Kommalapati Dinesh Kumar Anjana Devi Tangutur Source Type: research

Long non-coding RNA NKILA regulates expression of HSP90 α, NF-κB and β-catenin proteins in the MCF-7 breast cancer cell line
Mol Biol Rep. 2021 Jun 14. doi: 10.1007/s11033-021-06482-y. Online ahead of print.ABSTRACTNon-coding RNAs are increasingly being investigated and have shown great potential for diagnosis, prognosis and treatment of cancer. Thus, we have investigated a possible regulatory mechanism between NF-κB suppressor-NKILA, and HSP90, NF-κB, and β-catenin molecules in MCF-7 breast cancer cells. HSP90 is an important stress protein and together with β-catenin and NF-κB molecules can be responsible for cancer cell development. However, there is no comprehensive data available on the novel molecule NKILA unlik...
Source: Molecular Biology Reports - June 14, 2021 Category: Molecular Biology Authors: Mehmet Ali Tibatan Evren Önay Uçar Source Type: research

Molecular evolution of kalliklectin in teleost and identification of the novel type with eight apple domains in channel catfish, Ictalurus punctatus
Mol Biol Rep. 2021 Jun 14. doi: 10.1007/s11033-021-06446-2. Online ahead of print.ABSTRACTKalliklectin is a unique fish-specific lectin that demonstrates sequence similarity to mammalian plasma kallikrein and coagulation factor XI, which are not lectins but proteases. Reported fish kalliklectins and these mammalian proteases comprise four characteristic "apple domains" (APDs). Bioinformatics analysis revealed that Siluriformes species possess anomalous kalliklectins comprising 6 to 16 APDs. Complementary DNA cloning showed that the full-length nucleotide sequence of Ictalurus punctatus consists of 2240 bp that en...
Source: Molecular Biology Reports - June 14, 2021 Category: Molecular Biology Authors: Shigeyuki Tsutsui Asuka Yoshimura Yoshimi Kawakami Osamu Nakamura Source Type: research

Effects of dietary active soybean trypsin inhibitors on pancreatic weights, histology and expression of STAT3 and receptors for androgen and estrogen in different tissues of rats
This study investigated the effects of consuming active SBTI on pancreatic weights, histology, trypsinogen production and expression of STAT3, receptors for androgen (AR) and estrogen (ER) in pancreas, liver and uterus of rats. Weanling Sprague-Dawley rats were randomly divided into 3 groups (8 females and 8 males/group) and fed diets containing either 20% casein protein (Casein) or 20% soy protein (SP) in the presence of high (1.42 BAEE unit/µg, SP + SBTI) or low (0.2 BAEE unit/µg, SP-SBTI) levels of active SBTI for 8 weeks. Ingestion of SP + SBTI diet markedly increased pancreatic weights and trypsinogen content (p
Source: Molecular Biology Reports - June 14, 2021 Category: Molecular Biology Authors: Chao-Wu Xiao Carla Wood Lee Anne Cunningham Maryline Lalande Melissa Riding Source Type: research

Flooding or drought which one is more offensive on pepper physiology and growth?
Mol Biol Rep. 2021 Jun 13. doi: 10.1007/s11033-021-06437-3. Online ahead of print.ABSTRACTBoth extreme usage of water in agriculture i.e., drought and flooding affect physiological and growth aspects of the plant as well as gene expression undertaken in water absorption. These affect depend on the stress duration i.e., shock or gradual stress exposer. The factorial experiment based on CRD with 10 replicates was conducted to investigate the physiological and water relation as well as aquaporin expression in (Capsicum annuum L.). Drought stress was applied gradually from - 2, - 3, - 4 to - 5 MPa during 8 days but in shock st...
Source: Molecular Biology Reports - June 13, 2021 Category: Molecular Biology Authors: Zeinab Masoumi Maryam Haghighi Seyed Amir Hossein Jalali Source Type: research