Fabrication and evaluation of aptamer-conjugated paclitaxel-loaded magnetic nanoparticles for targeted therapy on breast cancer cells
Mol Biol Rep. 2021 Feb 26. doi: 10.1007/s11033-021-06199-y. Online ahead of print.ABSTRACTTargeted drug delivery vehicles make it possible to deliver anti-cancer drugs to the cells or tissues of interest. Aptamers are peptide or oligonucleotide molecules that can serve as targeting elements of drug carriers. In the current study, we evaluated the capacity of an aptamer-based drug carrier to deliver Paclitaxel (PTX) to cancer cells. After being synthesized, SPIONs@PTX-SYL3C aptamer was characterized using different methods, including differential light scattering (DLS), infrared spectroscopy (FTIR), Transmission electron mi...
Source: Molecular Biology Reports - February 26, 2021 Category: Molecular Biology Authors: Emad Khodadadi Soleiman Mahjoub Mehdi Sheikh Arabi Hossein Najafzadehvarzi Vahid Nasirian Source Type: research

Occurrence of Cotton leaf curl Multan virus and associated betasatellites with leaf curl disease of Bhut-Jolokia chillies (Capsicum chinense Jacq.) in India
Mol Biol Rep. 2021 Feb 26:1-10. doi: 10.1007/s11033-021-06223-1. Online ahead of print.ABSTRACTGeminiviridae comprises the largest family of plant viruses which causes severe crop losses in India. The highest pungency chilli Bhut-Jolokia or ghost pepper (Capsicum chinense Jaqc.) hails from North-East region of India and is used in many dishes to add flavors and also for its medicinal value. However, this chilli variety is also affected by viruses leading to crop and economic losses. The present study reports the identification of begomoviruses in the infected chilli Bhut-Jolokia leaf samples collected from eight different ...
Source: Molecular Biology Reports - February 26, 2021 Category: Molecular Biology Authors: Sneha Yogindran Manish Kumar Lingaraj Sahoo Keithellakpam Sanatombi Supriya Chakraborty Source Type: research

Development and characterization of Novel EST-based single-copy genic microsatellite DNA markers in white spruce and black spruce
Mol Biol Rep. 2021 Feb 26. doi: 10.1007/s11033-021-06231-1. Online ahead of print.ABSTRACTDue mainly to large genome size and prevalence of repetitive sequences in the nuclear genome of spruce (Picea Mill.), it is very difficult to develop single-copy genomic microsatellite markers. We have developed and characterized 25 polymorphic, single-copy genic microsatellites from white spruce (Picea glauca (Moench) Voss) EST sequences and determined their informativeness in white spruce and black spruce (Picea mariana (Mill.) B.S.P.) and inheritance in black spruce. White spruce EST sequences from NCBI dbEST were searched for the ...
Source: Molecular Biology Reports - February 26, 2021 Category: Molecular Biology Authors: Om P Rajora Ishminder K Mann Source Type: research

Fabrication and evaluation of aptamer-conjugated paclitaxel-loaded magnetic nanoparticles for targeted therapy on breast cancer cells
Mol Biol Rep. 2021 Feb 26. doi: 10.1007/s11033-021-06199-y. Online ahead of print.ABSTRACTTargeted drug delivery vehicles make it possible to deliver anti-cancer drugs to the cells or tissues of interest. Aptamers are peptide or oligonucleotide molecules that can serve as targeting elements of drug carriers. In the current study, we evaluated the capacity of an aptamer-based drug carrier to deliver Paclitaxel (PTX) to cancer cells. After being synthesized, SPIONs@PTX-SYL3C aptamer was characterized using different methods, including differential light scattering (DLS), infrared spectroscopy (FTIR), Transmission electron mi...
Source: Molecular Biology Reports - February 26, 2021 Category: Molecular Biology Authors: Emad Khodadadi Soleiman Mahjoub Mehdi Sheikh Arabi Hossein Najafzadehvarzi Vahid Nasirian Source Type: research

Occurrence of Cotton leaf curl Multan virus and associated betasatellites with leaf curl disease of Bhut-Jolokia chillies (Capsicum chinense Jacq.) in India
Mol Biol Rep. 2021 Feb 26. doi: 10.1007/s11033-021-06223-1. Online ahead of print.ABSTRACTGeminiviridae comprises the largest family of plant viruses which causes severe crop losses in India. The highest pungency chilli Bhut-Jolokia or ghost pepper (Capsicum chinense Jaqc.) hails from North-East region of India and is used in many dishes to add flavors and also for its medicinal value. However, this chilli variety is also affected by viruses leading to crop and economic losses. The present study reports the identification of begomoviruses in the infected chilli Bhut-Jolokia leaf samples collected from eight different place...
Source: Molecular Biology Reports - February 26, 2021 Category: Molecular Biology Authors: Sneha Yogindran Manish Kumar Lingaraj Sahoo Keithellakpam Sanatombi Supriya Chakraborty Source Type: research

Development and characterization of Novel EST-based single-copy genic microsatellite DNA markers in white spruce and black spruce
Mol Biol Rep. 2021 Feb 26. doi: 10.1007/s11033-021-06231-1. Online ahead of print.ABSTRACTDue mainly to large genome size and prevalence of repetitive sequences in the nuclear genome of spruce (Picea Mill.), it is very difficult to develop single-copy genomic microsatellite markers. We have developed and characterized 25 polymorphic, single-copy genic microsatellites from white spruce (Picea glauca (Moench) Voss) EST sequences and determined their informativeness in white spruce and black spruce (Picea mariana (Mill.) B.S.P.) and inheritance in black spruce. White spruce EST sequences from NCBI dbEST were searched for the ...
Source: Molecular Biology Reports - February 26, 2021 Category: Molecular Biology Authors: Om P Rajora Ishminder K Mann Source Type: research

EST-SSR marker development based on transcriptome sequencing and genetic analyses of Phoebe bournei (Lauraceae)
Mol Biol Rep. 2021 Feb 24. doi: 10.1007/s11033-021-06228-w. Online ahead of print.ABSTRACTHigh-throughput sequencing of the Phoebe bournei transcriptome was performed, and novel SSR markers were identified. A total of 73,518 nonredundant unigenes were assembled and annotated by sequence similarity searching in diverse public databases. A total of 40,853 SSRs were identified from 73,518 unigenes. Twenty-three pairs of polymorphic EST-SSR markers were selected from 98 markers and used for genetic analyses in 75 individuals from three P. bournei populations. The 23 pairs of markers could detect abundant genetic information fr...
Source: Molecular Biology Reports - February 25, 2021 Category: Molecular Biology Authors: Qi Zhou Peng-Yan Zhou Wen-Tao Zou Yin-Gang Li Source Type: research

The growth-inhibitory effects of pawpaw (Asimina triloba [L.] Dunal) roots, twigs, leaves, and fruit against human gastric (AGS) and cervical (HeLa) cancer cells and their anti-inflammatory activities
CONCLUSIONS: These findings indicate that pawpaw extracts are natural therapeutic agents that may be used for the prevention and treatment of gastric and cervical cancers, and encourage further studies on the anti-inflammatory potential of the pawpaw tree.PMID:33630206 | DOI:10.1007/s11033-021-06226-y (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - February 25, 2021 Category: Molecular Biology Authors: Jin-Sik Nam Seo-Yeon Park Seon-Ok Lee Hyo-Jeong Lee Hye-Lim Jang Young Ha Rhee Source Type: research

35S promoter-driven transgenes are variably expressed in different organs of Arabidopsis thaliana and in response to abiotic stress
This study shows that transgenes driven by a common constitutive promoter can exhibit marked variations in transcriptional activity depending on plant organ, physiological conditions, and in response to abiotic stress. Therefore, to ensure high and stable transgene activity, considerable attention should be given to the transgenic plant material and incubation conditions before harvesting the plant material.PMID:33630207 | DOI:10.1007/s11033-021-06235-x (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - February 25, 2021 Category: Molecular Biology Authors: Konstantin V Kiselev Olga A Aleynova Zlata V Ogneva Andrey R Suprun Alexandra S Dubrovina Source Type: research

Comparative transcriptomic analyses of normal and peloric mutant flowers in Cymbidium goeringii Rchb.f identifies differentially expressed genes associated with floral development
Conclusions: Our gene expression data may be used to study the regulatory mechanism of flower organ development in C. geringi.PMID:33630208 | DOI:10.1007/s11033-021-06216-0 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - February 25, 2021 Category: Molecular Biology Authors: Qi Shen Yue Chen Junwei Sun Qian Liu Chongbo Sun Source Type: research

EST-SSR marker development based on transcriptome sequencing and genetic analyses of Phoebe bournei (Lauraceae)
Mol Biol Rep. 2021 Feb 24. doi: 10.1007/s11033-021-06228-w. Online ahead of print.ABSTRACTHigh-throughput sequencing of the Phoebe bournei transcriptome was performed, and novel SSR markers were identified. A total of 73,518 nonredundant unigenes were assembled and annotated by sequence similarity searching in diverse public databases. A total of 40,853 SSRs were identified from 73,518 unigenes. Twenty-three pairs of polymorphic EST-SSR markers were selected from 98 markers and used for genetic analyses in 75 individuals from three P. bournei populations. The 23 pairs of markers could detect abundant genetic information fr...
Source: Molecular Biology Reports - February 25, 2021 Category: Molecular Biology Authors: Qi Zhou Peng-Yan Zhou Wen-Tao Zou Yin-Gang Li Source Type: research

The growth-inhibitory effects of pawpaw (Asimina triloba [L.] Dunal) roots, twigs, leaves, and fruit against human gastric (AGS) and cervical (HeLa) cancer cells and their anti-inflammatory activities
CONCLUSIONS: These findings indicate that pawpaw extracts are natural therapeutic agents that may be used for the prevention and treatment of gastric and cervical cancers, and encourage further studies on the anti-inflammatory potential of the pawpaw tree.PMID:33630206 | DOI:10.1007/s11033-021-06226-y (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - February 25, 2021 Category: Molecular Biology Authors: Jin-Sik Nam Seo-Yeon Park Seon-Ok Lee Hyo-Jeong Lee Hye-Lim Jang Young Ha Rhee Source Type: research

35S promoter-driven transgenes are variably expressed in different organs of Arabidopsis thaliana and in response to abiotic stress
This study shows that transgenes driven by a common constitutive promoter can exhibit marked variations in transcriptional activity depending on plant organ, physiological conditions, and in response to abiotic stress. Therefore, to ensure high and stable transgene activity, considerable attention should be given to the transgenic plant material and incubation conditions before harvesting the plant material.PMID:33630207 | DOI:10.1007/s11033-021-06235-x (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - February 25, 2021 Category: Molecular Biology Authors: Konstantin V Kiselev Olga A Aleynova Zlata V Ogneva Andrey R Suprun Alexandra S Dubrovina Source Type: research

Comparative transcriptomic analyses of normal and peloric mutant flowers in Cymbidium goeringii Rchb.f identifies differentially expressed genes associated with floral development
Conclusions: Our gene expression data may be used to study the regulatory mechanism of flower organ development in C. geringi.PMID:33630208 | DOI:10.1007/s11033-021-06216-0 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - February 25, 2021 Category: Molecular Biology Authors: Qi Shen Yue Chen Junwei Sun Qian Liu Chongbo Sun Source Type: research

Aspirin exacerbated respiratory disease (AERD): molecular and cellular diagnostic & amp; prognostic approaches
This study aimed to investigate the molecular mechanisms involved in AERD pathogenesis. Relevant literatures were identified by a PubMed search (2005-2019) of english language papers using the terms "Aspirin-exacerbated respiratory disease", "Allergic inflammation", "molecular mechanism" and "mutation". According to the significant role of inflammation in AERD development, ILC-2 is known as the most important cell in disease progression. ILC-2 produces cytokines that induce allergic reactions and also cause lipid mediators production, which activates mast cells and basophils, ultimat...
Source: Molecular Biology Reports - February 24, 2021 Category: Molecular Biology Authors: Habib Hybar Najmaldin Saki Mohsen Maleknia Mana Moghaddasi Armin Bordbar Maliheh Naghavi Source Type: research

CREB-binding protein (CREBBP) and preeclampsia: a new promising target gene
Mol Biol Rep. 2021 Feb 24. doi: 10.1007/s11033-021-06215-1. Online ahead of print.ABSTRACTPreeclampsia (PE) is a major complication of pregnancy and remains a leading cause of neonatal and maternal mortality worldwide. Several studies have revealed that the incidence of preeclampsia is high in mothers who carried a fetus with Rubinstein-Taybi Syndrome due to the mutation in CREBBP. We aimed to compare the expression level of the CERBBP gene between preeclamptic and healthy placenta in our study. The expression level of CREBBP gene was evaluated in a total of one hundred placental biopsies from PE patients and healthy pregn...
Source: Molecular Biology Reports - February 24, 2021 Category: Molecular Biology Authors: Hossein Sadeghi Sahra Esmkhani Reihaneh Pirjani Mona Amin-Beidokhti Milad Gholami Ghasem Azizi Tabesh Mohammad Reza Ghasemi Latif Gachkar Reza Mirfakhraie Source Type: research

The effects of different training modalities on monocarboxylate transporters MCT1 and MCT4, hypoxia inducible factor-1 α (HIF-1α), and PGC-1α gene expression in rat skeletal muscles
This study aimed to investigate the effects of moderate intensity continuous training (MICT) and isocaloric high intensity interval training (HIIT) on gene expression of monocarboxylate transporter 1 (MCT1) and 4 (MCT4), Peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), and hypoxia inducible factor-1α (HIF-1α) in soleus and extensor digitorum longus (EDL) skeletal muscles of rats. Thirty male Sprague-Dawley rats were divided into 3 groups of control, MICT, and HIIT. Training protocols were performed according to the principle of overload for 8 weeks and 5 sessions per week....
Source: Molecular Biology Reports - February 24, 2021 Category: Molecular Biology Authors: Akbar Ahmadi Dariush Sheikholeslami-Vatani Saeed Ghaeeni Maryam Baazm Source Type: research

Aspirin exacerbated respiratory disease (AERD): molecular and cellular diagnostic & amp; prognostic approaches
This study aimed to investigate the molecular mechanisms involved in AERD pathogenesis. Relevant literatures were identified by a PubMed search (2005-2019) of english language papers using the terms "Aspirin-exacerbated respiratory disease", "Allergic inflammation", "molecular mechanism" and "mutation". According to the significant role of inflammation in AERD development, ILC-2 is known as the most important cell in disease progression. ILC-2 produces cytokines that induce allergic reactions and also cause lipid mediators production, which activates mast cells and basophils, ultimat...
Source: Molecular Biology Reports - February 24, 2021 Category: Molecular Biology Authors: Habib Hybar Najmaldin Saki Mohsen Maleknia Mana Moghaddasi Armin Bordbar Maliheh Naghavi Source Type: research

CREB-binding protein (CREBBP) and preeclampsia: a new promising target gene
Mol Biol Rep. 2021 Feb 24. doi: 10.1007/s11033-021-06215-1. Online ahead of print.ABSTRACTPreeclampsia (PE) is a major complication of pregnancy and remains a leading cause of neonatal and maternal mortality worldwide. Several studies have revealed that the incidence of preeclampsia is high in mothers who carried a fetus with Rubinstein-Taybi Syndrome due to the mutation in CREBBP. We aimed to compare the expression level of the CERBBP gene between preeclamptic and healthy placenta in our study. The expression level of CREBBP gene was evaluated in a total of one hundred placental biopsies from PE patients and healthy pregn...
Source: Molecular Biology Reports - February 24, 2021 Category: Molecular Biology Authors: Hossein Sadeghi Sahra Esmkhani Reihaneh Pirjani Mona Amin-Beidokhti Milad Gholami Ghasem Azizi Tabesh Mohammad Reza Ghasemi Latif Gachkar Reza Mirfakhraie Source Type: research

The effects of different training modalities on monocarboxylate transporters MCT1 and MCT4, hypoxia inducible factor-1 α (HIF-1α), and PGC-1α gene expression in rat skeletal muscles
This study aimed to investigate the effects of moderate intensity continuous training (MICT) and isocaloric high intensity interval training (HIIT) on gene expression of monocarboxylate transporter 1 (MCT1) and 4 (MCT4), Peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), and hypoxia inducible factor-1α (HIF-1α) in soleus and extensor digitorum longus (EDL) skeletal muscles of rats. Thirty male Sprague-Dawley rats were divided into 3 groups of control, MICT, and HIIT. Training protocols were performed according to the principle of overload for 8 weeks and 5 sessions per week....
Source: Molecular Biology Reports - February 24, 2021 Category: Molecular Biology Authors: Akbar Ahmadi Dariush Sheikholeslami-Vatani Saeed Ghaeeni Maryam Baazm Source Type: research

Impact of Reck expression and promoter activity in neuronal in vitro differentiation
Mol Biol Rep. 2021 Feb 22. doi: 10.1007/s11033-021-06175-6. Online ahead of print.ABSTRACTReck (REversion-inducing Cysteine-rich protein with Kazal motifs) tumor suppressor gene encodes a multifunctional glycoprotein which inhibits the activity of several matrix metalloproteinases (MMPs), and has the ability to modulate the Notch and canonical Wnt pathways. Reck-deficient neuro-progenitor cells undergo precocious differentiation; however, modulation of Reck expression during progression of the neuronal differentiation process is yet to be characterized. In the present study, we demonstrate that Reck expression levels are i...
Source: Molecular Biology Reports - February 23, 2021 Category: Molecular Biology Authors: Marina Trombetta-Lima Thais Assis-Ribas Ricardo C Cintra Joana D Campeiro Juliano R Guerreiro Sheila M B Winnischofer Isis C C Nascimento Henning Ulrich Mirian A F Hayashi Mari C Sogayar Source Type: research

Promoter hypomethylation as potential confounder of Ras gene overexpression and their clinical significance in subsets of urothelial carcinoma of bladder
Mol Biol Rep. 2021 Feb 23. doi: 10.1007/s11033-021-06227-x. Online ahead of print.ABSTRACTOverexpression of normal Ras and its aberrant CpG island methylation in the promoter regions have been shown to direct cells for uncontrolled abnormal growth and bladder tumor formation and therefore, fetched recent attention as a marker of diagnosis and prognosis to predict the biological behavior of urothelial carcinoma of bladder (UCB). Methylation pattern at CpG islands of the promoter regions of rat sarcoma (Ras) gene homologues namely Kristen-Ras (K-Ras), Harvey (H-Ras), and Neuroblastoma (N-Ras) were examined by methylation spe...
Source: Molecular Biology Reports - February 23, 2021 Category: Molecular Biology Authors: Kiran Tripathi Apul Goel Atin Singhai Minal Garg Source Type: research

Glucose-6-phosphate dehydrogenase deficiency and hydroxychloroquine in the COVID-19 era: a mini review
This study aims to answer the question raised by Mastroianni et al. "Hydroxychloroquine: Culprit or Innocent Bystander in G6PD-Deficient Patients with COVID-19?", reporting all cases of HCQ in G6PD deficient COVID-19 patients published on PubMed (pubmed.ncbi.nlm.nih.gov), in addition to the Mastroianni's patient. In our opinion, after an accurate revision of these cases and responding the question raised by Mastroianni et al., we believe that it is difficult to reach a final verdict about the definitive role of HCQ in these patients. The COVID-19 pandemic has reopened attention on HCQ use and G6PDD. G6PD status i...
Source: Molecular Biology Reports - February 23, 2021 Category: Molecular Biology Authors: Maria Elisabetta Onori Claudio Ricciardi Tenore Andrea Urbani Angelo Minucci Source Type: research

Disruption of p97/VCP induces autophagosome accumulation, cell cycle arrest and apoptosis in human choriocarcinoma cells
Mol Biol Rep. 2021 Feb 23. doi: 10.1007/s11033-021-06225-z. Online ahead of print.ABSTRACTGestational choriocarcinoma is aggressive trophoblastic disease. The development, progression and the cure of this disease is not well-established. p97/Valosin containing protein has been shown to play critical roles in many cellular processes. In various cancers, higher expression of p97/VCP has been reported and targeting of p97/VCP with its spesific inhibitors or siRNA's (siVCP) in cancer therapy was suggested. However, no study is avaible about the expression and function of p97/VCP in gestational choriocarcinoma. Hence, the aim o...
Source: Molecular Biology Reports - February 23, 2021 Category: Molecular Biology Authors: Raziye Desdicioglu Cansu Sahin Filiz Yavuz Sevil Cayli Source Type: research

ABC transporter superfamily. An updated overview, relevance in cancer multidrug resistance and perspectives with personalized medicine
Mol Biol Rep. 2021 Feb 22. doi: 10.1007/s11033-021-06155-w. Online ahead of print.ABSTRACTThe ATP binding-cassette superfamily corresponds the mostly transmembrane transporters family found in humans. These proteins actively transport endogenous and exogenous substrates through biological membranes in body tissues, so they have an important role in the regulation of many physiological functions necessary for human homeostasis, as well as in response regulation to several pharmacological substrates. The development of multidrug resistance has become one of the main troubles in conventional chemotherapy in different illnesse...
Source: Molecular Biology Reports - February 22, 2021 Category: Molecular Biology Authors: P érez-De Marcos Juan-Carlos P érez-Pineda Perla-Lidia M éndez-Morales Stephanie-Talia Arellano-Mendoza M ónica-Griselda Torres-Esp índola Luz-María Source Type: research

PTPN22 gene functional polymorphism (rs2476601) in older adults with frailty syndrome
Mol Biol Rep. 2021 Feb 21. doi: 10.1007/s11033-021-06212-4. Online ahead of print.ABSTRACTThe frailty syndrome is a common clinical marker of vulnerability in older adults conducive to an overall decline in inflammatory stress responsiveness; yet little is known about the genetic risk factors for frailty in elderly. Our aim was to investigate the association between the rs2476601 polymorphism in PTPN22 gene and susceptibility to frailty in Mexican older adults. Data included 630 subjects 70 and older from The Coyoacán cohort, classified as frail, pre-frail, and non-frail following Fried's criteria. Sociodemographic ...
Source: Molecular Biology Reports - February 21, 2021 Category: Molecular Biology Authors: Rub én Rabaneda-Bueno Norma Torres-Carrillo Jos é Alberto Ávila-Funes Luis Miguel Guti érrez-Robledo Thal ía Gabriela Pérez-Suárez Jos é Luis Acosta Sara Torres-Castro Ana Lilia Fletes-Rayas Itzae Gutierrez-Hurtado Elena Sandoval-Pinto Rosa Cremad Source Type: research

The effect of salt stress on the production of apocarotenoids and the expression of genes related to their biosynthesis in saffron
Mol Biol Rep. 2021 Feb 21. doi: 10.1007/s11033-021-06219-x. Online ahead of print.ABSTRACTSaffron stigmas are widely used as food additives and as traditional medicine in Iran and many other countries. The unique taste, flavor and pharmaceutical properties of saffron stigmas are due to the presence of three apocarotenoids secondary metabolites crocin, picrocrocin and safranal. There is limited knowledge about the effect of environmental stresses on the metabolism of apocarotenoids in saffron. We analyzed the content of crocin and picrocrocin and the expression of key genes of apocarotenoid biosynthesis pathways (CsCCD2, Cs...
Source: Molecular Biology Reports - February 21, 2021 Category: Molecular Biology Authors: Fateme Sadat Moslemi Atousa Vaziri Golandam Sharifi Javad Gharechahi Source Type: research

Hippophae rhamnoides mediate gene expression profiles against keratinocytes infection of Staphylococcus aureus
This study aims to investigate the S. aureus mediated molecular modulation, and the effect of HR in reversing the deleterious impact of S. aureus in keratinocytes. Human keratinocyte (HaCaT) cells were cultured in DMEM, supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Subcultures were divided into three flasks: control with no S. aureus and extract (C), S. aureus infected (SA) and S. aureus infected cells and extract (SE). RNA was isolated and microarray analysis was performed. The data was annotated using GO functional analysis and DAVID functional annotation. For each comparison group, signi...
Source: Molecular Biology Reports - February 20, 2021 Category: Molecular Biology Authors: Humaira Shah Hafiz Abdullah Shakir Sher Zaman Safi Abid Ali Source Type: research

Impact of heat stress responsive factors on growth and physiology of cotton (Gossypium hirsutum L.)
Mol Biol Rep. 2021 Feb 20. doi: 10.1007/s11033-021-06217-z. Online ahead of print.ABSTRACTPakistan ranked highest with reference to average temperatures in cotton growing areas of the world. The heat waves are becoming more intense and unpredictable due to climate change. Identification of heat tolerant genotypes requires comprehensive screening using molecular, physiological and morphological analysis. Heat shock proteins play an important role in tolerance against heat stress. In the current study, eight heat stress responsive factors, proteins and genes (HSFA2, GHSP26, GHPP2A, HSP101, HSC70-1, HSP3, APX1 and ANNAT8) wer...
Source: Molecular Biology Reports - February 20, 2021 Category: Molecular Biology Authors: Muhammad Asif Saleem Waqas Malik Abdul Qayyum Sami Ul-Allah Muhammad Qadir Ahmad Hammad Afzal Muhammad Waqas Amjid Muhammad Farjad Ateeq Zia Ullah Zia Source Type: research

Research progress of natural compounds in anti-liver fibrosis by affecting autophagy of hepatic stellate cells
Mol Biol Rep. 2021 Feb 20. doi: 10.1007/s11033-021-06171-w. Online ahead of print.ABSTRACTChronic liver diseases caused by various pathogenesis are marked by inflammatory infiltration and wound healing reaction, while their normal regeneration ability is impaired. The unbalance between the generation and the degradation of extracellular matrix (ECM) leads to collagen accumulation and develops into liver fibrosis. Inflammation, oxidative stress, and autophagy interact closely in the pathogenesis of hepatic fibrosis. Reactive Oxygen Species (ROS) can not only stimulate Kupffer cells to release massive inflammatory factors, b...
Source: Molecular Biology Reports - February 20, 2021 Category: Molecular Biology Authors: Yongxiang Shu Xuyou Liu Haifeng Huang Qi Wen Jianchang Shu Source Type: research

Impact of heat stress responsive factors on growth and physiology of cotton (Gossypium hirsutum L.).
Abstract Pakistan ranked highest with reference to average temperatures in cotton growing areas of the world. The heat waves are becoming more intense and unpredictable due to climate change. Identification of heat tolerant genotypes requires comprehensive screening using molecular, physiological and morphological analysis. Heat shock proteins play an important role in tolerance against heat stress. In the current study, eight heat stress responsive factors, proteins and genes (HSFA2, GHSP26, GHPP2A, HSP101, HSC70-1, HSP3, APX1 and ANNAT8) were evaluated morphologically and physiologically for their role in heat s...
Source: Molecular Biology Reports - February 20, 2021 Category: Molecular Biology Authors: Saleem MA, Malik W, Qayyum A, Ul-Allah S, Ahmad MQ, Afzal H, Amjid MW, Ateeq MF, Zia ZU Tags: Mol Biol Rep Source Type: research

Research progress of natural compounds in anti-liver fibrosis by affecting autophagy of hepatic stellate cells.
Abstract Chronic liver diseases caused by various pathogenesis are marked by inflammatory infiltration and wound healing reaction, while their normal regeneration ability is impaired. The unbalance between the generation and the degradation of extracellular matrix (ECM) leads to collagen accumulation and develops into liver fibrosis. Inflammation, oxidative stress, and autophagy interact closely in the pathogenesis of hepatic fibrosis. Reactive Oxygen Species (ROS) can not only stimulate Kupffer cells to release massive inflammatory factors, but induce autophagy. However, the latter may suppress inflammatory react...
Source: Molecular Biology Reports - February 20, 2021 Category: Molecular Biology Authors: Shu Y, Liu X, Huang H, Wen Q, Shu J Tags: Mol Biol Rep Source Type: research

Hippophae rhamnoides mediate gene expression profiles against keratinocytes infection of Staphylococcus aureus.
This study aims to investigate the S. aureus mediated molecular modulation, and the effect of HR in reversing the deleterious impact of S. aureus in keratinocytes. Human keratinocyte (HaCaT) cells were cultured in DMEM, supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Subcultures were divided into three flasks: control with no S. aureus and extract (C), S. aureus infected (SA) and S. aureus infected cells and extract (SE). RNA was isolated and microarray analysis was performed. The data was annotated using GO functional analysis and DAVID functional annotation. For each comparison group, signi...
Source: Molecular Biology Reports - February 19, 2021 Category: Molecular Biology Authors: Shah H, Shakir HA, Safi SZ, Ali A Tags: Mol Biol Rep Source Type: research

Identification and characterization of the EXPA7, EXPA18 and EXT10 genes in Turbinicarpus lophophoroides (Werderm.) Buxb. & Backeb; and their expression analysis in the root under abiotic stress.
In this study, we identified and characterized the expansin7, expansin18 and extensin10 genes in T. lophophoroides. Bioinformatic analysis indicated that the expansin sequences contained the motifs: HTFYG, HFD, YRR, VPC and YW; and certain conserved cysteine (C) residues. Regarding extensin10, the sequence contains the conserved SPPPP (SP4), YYS and YV motifs. The expression analysis in adventitious and transformed roots under osmotic stress (300 mM mannitol), heat (37 °C) and cold (4 °C); shows a higher expression of TlExpA18 in both roots, a decrease in TlExpA7 in transformed roots and a null expre...
Source: Molecular Biology Reports - February 19, 2021 Category: Molecular Biology Authors: Martínez-Vázquez JP, Loera-Muro A, Gómez-Aguirre YA, Morales-Domínguez JF Tags: Mol Biol Rep Source Type: research

Nanomicellar curcuminoids attenuates renal ischemia/reperfusion injury in rat through prevention of apoptosis and downregulation of MAPKs pathways.
Abstract Renal ischemia/reperfusion (I/R) injury is considered as a main problem in clinical practice. Curcuminoids, the active constituents of turmeric, seem to have potential renoprotective effects. However, the poor bioavailability of curcuminoids restricts their therapeutic effects. In the present study, the effect of nanomicellar curcuminoids (NC) treatment on renal function, histology, total antioxidant capacity (TAC), total oxidative stress (TOS), caspase-3 level as well as mitogen activated protein kinases (MAPKs: JNK, p38 and ERK) phosphorylation were evaluated following renal I/R. Adult male Sprague-Dawl...
Source: Molecular Biology Reports - February 19, 2021 Category: Molecular Biology Authors: Karimi Z, SoukhakLari R, Rahimi-Jaberi K, Esmaili Z, Moosavi M Tags: Mol Biol Rep Source Type: research

Astragaloside IV inhibits palmitic acid-induced apoptosis through regulation of calcium homeostasis in mice podocytes.
Abstract Loss of podocytes is a hallmark of diabetic nephropathy, and a growing body of evidence indicates that podocytes are susceptible to palmitic acid (PA). We have previously shown that AS-IV inhibited PA-induced podocyte apoptosis by activating sarcoendoplasmic reticulum Ca2+ ATPase (SERCA), which indicate calcium regulation may involve in the process. Immunofluorescence staining, Western blot and flow cytometry were used to measure the protective efficacy of AS-IV to ameliorate PA-induced ER stress and podocyte apoptosis. Meanwhile, AS-IV inhibited cytochrome c release, decreased mitochondrial membrane pote...
Source: Molecular Biology Reports - February 19, 2021 Category: Molecular Biology Authors: Zang Y, Liu S, Cao A, Shan X, Deng W, Li Z, Wang H, Wang Y, Wang L, Peng W Tags: Mol Biol Rep Source Type: research

Correction to: A protocol for umbilical cord tissue cryopreservation as a source of mesenchymal stem cells.
PMID: 33606152 [PubMed - as supplied by publisher] (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - February 19, 2021 Category: Molecular Biology Authors: Raffo D, Maglioco A, Fernandez Sasso D Tags: Mol Biol Rep Source Type: research

Two different begomovirus species are associated with yellow vein mosaic disease of okra in Sri Lanka.
Abstract Yellow vein mosaic disease is the major biotic constraint of okra cultivation in Sri Lanka. Identification and detailed molecular characterization of associated pathogen is needed for effective disease management. The genome of the begomovirus and betasatellite were amplified in symptomatic plant samples using specific degenerate primers. DNA-A genome of twelve isolates representing different locations in Sri Lanka were cloned, sequenced and deposited in GenBank database (Accession No- KX698087- KX698092 and MH455207- MH455212). Size of the complete nucleotide sequences ranged from 2735 to 2786 bp. T...
Source: Molecular Biology Reports - February 18, 2021 Category: Molecular Biology Authors: Jeyaseelan TC, De Costa DM, Shaw MW Tags: Mol Biol Rep Source Type: research

SMAD2, SMAD3 and TGF- β GENE expressions in women suffering from urge urinary incontinence and pelvic organ prolapse.
This study included 10 patients who had been diagnosed with POP and UUI in the study group and 14 asymptomatic women without complaints of POP and UUI in the control group. Biopsy samples were collected from bilateral USL tissues during vaginal or abdominal hysterectomy. Total RNA was extracted from USL tissue and analyzed by qPCR. The protein expression levels were also analyzed with ELISA. In UUI patients, SMAD3 and TGF-ß1 gene expression levels significantly decreased compared to the control patients (p = 0.008 and p = 0.006, respectively). SMAD2 mRNA levels did not differ between the s...
Source: Molecular Biology Reports - February 18, 2021 Category: Molecular Biology Authors: Akin MN, Sivaslioglu AA, Edgunlu T, Kasap B, Celik SK Tags: Mol Biol Rep Source Type: research

Antimicrobial activity of Actinobacteria isolated from dry land soil in Yazd, Iran.
Abstract Historically, many important secondary metabolites including antibiotics used in clinic are purified from the cultural broths of Actinobacteria, which were inhabited in soil. Yazd is located in the center of Iran, the south of the Dasht-e Kavir and the west of the Dasht-e Lut; accordingly it has a hot, dry climate with long summers. In the present study, 18 strains of Actinobacteria isolated from 60 soil samples from Yazd-Iran. Pure isolates were screened for antibacterial activity against the ATCC strains by using two methods: single line streak method and spot inoculation method. ATCC strains include fo...
Source: Molecular Biology Reports - February 18, 2021 Category: Molecular Biology Authors: Majidzadeh M, Heidarieh P, Fatahi-Bafghi M, Vakili M Tags: Mol Biol Rep Source Type: research

Tissue micro-RNAs associated with colorectal cancer prognosis: a systematic review.
Abstract Colorectal cancer (CRC) is a multifactorial disease commonly diagnosed worldwide, with high mortality rates. Several studies demonstrate important associations between differential expression of micro-RNAs (miRs) and the prognosis of CRC. The present study aimed to identify differentially expressed tissue miRs associated with prognostic factors in CRC patients, through a systematic review of the Literature. Using the PubMed database, Cochrane Library and Web of Science, studies published in English evaluating miRs differentially expressed in tumor tissue and significantly associated with the prognostic as...
Source: Molecular Biology Reports - February 17, 2021 Category: Molecular Biology Authors: Dos Santos IL, Penna KGBD, Dos Santos Carneiro MA, Libera LSD, Ramos JEP, Saddi VA Tags: Mol Biol Rep Source Type: research

Catalytic antibodies in the bone marrow and other organs of Th mice during spontaneous development of experimental autoimmune encephalomyelitis associated with cell differentiation.
Abstract Exact mechanisms of autoimmune disease development are still yet unknown. However, it is known that the development of autoimmune diseases is associated with defects in the immune system, namely, the violation of the bone marrow hematopoietic stem cells (HSCs) differentiation profiles. Different characteristics of autoimmune reaction development in experimental autoimmune encephalomyelitis (EAE) prone Th mice characterizing T-lymphocytes response were analyzed using standard approaches. Profiles of several HSCs differentiation of bone marrow (BFU-E, CFU-E, CFU-GM, CFU-GEMM, T- and B-lymphocytes) of Th mal...
Source: Molecular Biology Reports - February 17, 2021 Category: Molecular Biology Authors: Aulova KS, Urusov AE, Toporkova LB, Sedykh SE, Shevchenko YA, Tereshchenko VP, Sennikov SV, Budde T, Meuth SG, Orlovskaya IA, Nevinsky GA Tags: Mol Biol Rep Source Type: research

Silencing of ZFP36L2 increases sensitivity to temozolomide through G2/M cell cycle arrest and BAX mediated apoptosis in GBM cells.
Abstract Despite the advancements in primary brain tumour diagnoses and treatments, the mortality rate remains high, particularly in glioblastoma (GBM). Chemoresistance, predominantly in recurrent cases, results in decreased mean survival of patients with GBM. We aimed to determine the chemosensitisation and oncogenic characteristics of zinc finger protein 36-like 2 (ZFP36L2) in LN18 GBM cells via RNA interference (RNAi) delivery. We conducted a meta-analysis of microarray datasets and RNAi screening using pooled small interference RNA (siRNA) to identify the druggable genes responsive to GBM chemosensitivity. Tem...
Source: Molecular Biology Reports - February 15, 2021 Category: Molecular Biology Authors: Che Mat MF, Mohamad Hanif EA, Abdul Murad NA, Ibrahim K, Harun R, Jamal R Tags: Mol Biol Rep Source Type: research

A synthetic peptide as an allosteric inhibitor of human arginase I and II.
Abstract Arginine metabolism mediated by arginases plays a critical role in cell and tissue function. The arginine hydrolysis is deeply involved in the urea cycle, which helps the kidney excrete ammonia from blood. Upregulation of arginases affects microenvironment stability due to the presence of excess urea in blood. To regulate the arginase activities properly, a synthetic peptide based on the structure of human arginase I was designed and assessed. Preliminary data shows it inhibits human arginase I and II with an IC50 of 2.4 ± 0.3 and 1.8 ± 0.1 mmol, respectively...
Source: Molecular Biology Reports - February 15, 2021 Category: Molecular Biology Authors: Gao K, Lunev S, van den Berg MPM, Al-Dahmani ZM, Evans S, Mertens DALJ, Meurs H, Gosens R, Groves MR Tags: Mol Biol Rep Source Type: research

Are functional variants of the microRNA-146a gene associated with primary knee OA? Evidence in Mexican mestizo population.
This study aimed to analyze the association between two functional variants of the miR-146a gene and primary knee OA in Mexican mestizo population. Methods and Results. A case-control study was conducted with cases defined as individuals aged ≥ 40 years with primary knee OA grade ≥ 2, according to the Kellgren-Lawrence system. Controls were volunteers with no primary knee OA with radiographic grade 
Source: Molecular Biology Reports - February 15, 2021 Category: Molecular Biology Authors: Miranda-Duarte A, Borgonio-Cuadra VM, González-Huerta NC, Rojas-Toledo EX, Ahumada-Pérez JF, Morales-Hernández E, Pérez-Hernández N, Rodríguez-Pérez JM, Vargas-Alarcón G Tags: Mol Biol Rep Source Type: research

Isolation and characterization of a novel antimicrobial oxatetracyclo ketone from Bacillus stercoris MBTDCMFRI Ba37 isolated from the tropical estuarine habitats of Cochin.
Abstract Antimicrobial compounds from the safest source have gained greater relevance because of their wide spectrum of possible applications, especially in aquaculture industry, where pathogenic threat and antibacterial resistance are serious concerns. Bacillus stercoris MBTDCMFRI Ba37 isolated from mangrove environment of tropical estuarine habitats of Cochin exhibited a wide spectrum of antibacterial activity against major aquaculture pathogens belonging to genus Vibrio and Aeromonas. The structural characterization of the antibacterial compound from this strain may help in identifying their role as a biocontro...
Source: Molecular Biology Reports - February 15, 2021 Category: Molecular Biology Authors: Nair AV, Praveen NK, Joseph N, Leo AM, Vijayan KK Tags: Mol Biol Rep Source Type: research

Toxic milk mice models of Wilson's disease.
Abstract Wilson's disease (WD) is a rare genetic disorder inherited as an autosomal recessive trait. The signs and symptoms of this disease are related to dysfunctional ATP7B protein which leads to copper accumulation and cellular damage. The organs that are most commonly affected by WD are the liver and brain. The dysfunctional ATP7B homolog has previously been identified in many different species, including two naturally occurring murine models called toxic milk mice. The aim of this paper was to compare the toxic milk mouse described by Rauch (tx) to that from Jackson Laboratory (txJ) through a review of studie...
Source: Molecular Biology Reports - February 15, 2021 Category: Molecular Biology Authors: Hadrian K, Przybyłkowski A Tags: Mol Biol Rep Source Type: research

Profilin-1; a novel regulator of DNA damage response and repair machinery in keratinocytes.
Abstract Profilin-1 (PFN1) regulates actin polymerization and cytoskeletal growth. Despite the essential roles of PFN1 in cell integration, its subcellular function in keratinocyte has not been elucidated yet. Here we characterize the specific regulation of PFN1 in DNA damage response and repair machinery. PFN1 depletion accelerated DNA damage-mediated apoptosis exhibiting PTEN loss of function instigated by increased phosphorylated inactivation followed by high levels of AKT activation. PFN1 changed its predominant cytoplasmic localization to the nucleus upon DNA damage and subsequently restored the cytoplasmic c...
Source: Molecular Biology Reports - February 15, 2021 Category: Molecular Biology Authors: Lee CJ, Yoon MJ, Kim DH, Kim TU, Kang YJ Tags: Mol Biol Rep Source Type: research

Acute oral treatment with resveratrol and Lactococcus Lactis Subsp. Lactis decrease body weight and improve liver proinflammatory markers in C57BL/6 mice.
s SHS Abstract The present study aimed to evaluate the effects of resveratrol, a nutraceutical polyphenol, and Lactococcus lactis (bacteria probiotic), on metabolic parameters and hepatic proinflammatory markers expression. C57BL/6 mice were divided into 4 groups: Standard (ST), Lactococcus lactis (LL), Resveratrol (RSV), and Lactococcus lactis plus resveratrol (LL + RSV). Lactococcus lactis and resveratrol were administered by orogastric gavage. Blood parameters were assessed (total cholesterol, triglycerides, ALT and AST). IL-6 mRNA expression was evaluated by Real-time PCR and TNF-α protein ...
Source: Molecular Biology Reports - February 14, 2021 Category: Molecular Biology Authors: Mendes KL, Lelis DF, de Freitas DF, da Silveira LH, de Paula AMB, Guimarães ALS, Oliveira JR, Andrade MC, Nobre SAM, Santos SHS Tags: Mol Biol Rep Source Type: research

The nearly complete mitogenome of the Southeast Asian firefly Pteroptyx tener (Coleoptera: Lampyridae).
Abstract The estuarine firefly, Pteroptyx tener, aggregates in the thousands in mangrove trees lining tidal rivers in Southeast Asia where they engage one another in a nocturnal, pre-mating ritual of synchronised courtship flashes. Unfortunately, populations of the species by virtue of being restricted to isolated estuarine rivers systems in the region, are at risk of genetic isolation. Because of this concern we undertook the task of sequencing and characterising the mitochondrial DNA genome of P. tener, as the first step towards helping us to characterise and better understand their genetic diversity. We sequenc...
Source: Molecular Biology Reports - February 13, 2021 Category: Molecular Biology Authors: Cheng S, Mat-Isa MN, Sapian IS, Ishak SF Tags: Mol Biol Rep Source Type: research