Query: siRNA

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Total 14895 results found since Jan 2013.

NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway.
CONCLUSIONS: Nck1 promotes the angiogenesis-inducing capacity of CSCC via the Rac1/PAK1/MMP2 signal pathway. PMID: 30442385 [PubMed - as supplied by publisher]
Source: Gynecologic Oncology - November 13, 2018 Category: Cancer & Oncology Authors: Xia P, Huang M, Zhang Y, Xiong X, Yan M, Xiong X, Yu W, Song E Tags: Gynecol Oncol Source Type: research

Mammalian Target of Rapamycin Complex 1 Activation Disrupts the Low-Density Lipoprotein Receptor Pathway: A Novel Mechanism for Extracellular Matrix Accumulation in Human Peritoneal Mesothelial Cells.
Abstract Peritoneal fibrosis (PF) is characterized by progressive extracellular matrix (ECM) accumulation. Increasing evidence has suggested that ECM synthesis was increased in human peritoneal mesothelial cells (HPMCs) under high-glucose conditions, but the effects of high-glucose peritoneal dialysis solution (PDS) on ECM synthesis have not been fully elucidated. The aim of this study was to explore the potential mechanisms of high-glucose PDS-induced production of ECM in HPMCs. HPMCs were stimulated by high-glucose PDS. The activity of mammalian target of rapamycin complex 1 (mTORC1) was inhibited by rapamycin...
Source: American Journal of Nephrology - November 13, 2018 Category: Urology & Nephrology Authors: Liu J, Zhu W, Jiang CM, Feng Y, Xia YY, Zhang QY, Xu PF, Zhang M Tags: Am J Nephrol Source Type: research

Inhibition of Calcium/Calmodulin ‐Dependent Protein Kinase Kinase (CaMKK) Exacerbates Impairment of Endothelial Cell and Blood‐Brain Barrier after Stroke
This article is protected by copyright. All rights reserved.
Source: European Journal of Neuroscience - November 13, 2018 Category: Neuroscience Authors: Ping Sun, Fan Bu, Jia ‐Wei Min, Yashasvee Munshi, Matthew D. Howe, Lin Liu, Edward C.  Koellhoffer, Li Qi, Louise D.  McCullough, Jun Li Tags: Research Report Source Type: research

Reciprocal regulation of pro-inflammatory Annexin A2 and anti-inflammatory Annexin A1 in the pathogenesis of rheumatoid arthritis.
Abstract Annexin A2 has been implicated in several immune modulated diseases including Rheumatoid arthritis (RA) pannus formation. The most relied treatment option for RA pathogenesis is glucocorticoids. Glucocorticoids regulate the synthesis, phosphorylation and cellular deposition of Annexin A1. This annexin mediates the anti-inflammatory actions of glucocorticoids. These two first characterized members of annexin superfamily proteins acts reciprocally, one as an anti-inflammatory and the other proinflammatory in nature. The possibility of these molecules as soluble biomarkers and as an upstream regulator of maj...
Source: Molecular Biology Reports - November 13, 2018 Category: Molecular Biology Authors: Haridas V, Shetty P, Sarathkumar E, Bargale A, Vishwanatha JK, Patil V, Dinesh US Tags: Mol Biol Rep Source Type: research

Activated toll-like receptor 4 is involved in oridonin-induced phagocytosis via promotion of migration and autophagy-lysosome pathway in RAW264.7 macrophages.
Abstract In our previous study, we demonstrated that oridonin enhances phagocytosis of apoptotic bodies by macrophage-like cells by inducing autophagy. However, the direct sensor of autophagy and the key event controlling phagocytosis remains unknown. Herein, we showed that Toll-like receptor 4 (TLR4), known to mediate immune responses, was activated by oridonin. Activated TLR4 contributes to phagocytosis of apoptotic cells by RAW264.7 macrophages. Indeed, inhibition or small interfering RNA (siRNA) silencing of TLR4 significantly attenuated oridonin-induced phagocytosis. Inhibition of TLR4 also decreased the leve...
Source: International Immunopharmacology - November 13, 2018 Category: Allergy & Immunology Authors: Zang L, Wang J, Ren Y, Liu W, Yu Y, Zhao S, Otkur W, Zhao Y, Hayashi T, Tashiro SI, Onodera S, Ikejima T Tags: Int Immunopharmacol Source Type: research

Characterisation of the NRF2 transcriptional network and its response to chemical insult in primary human hepatocytes: implications for prediction of drug-induced liver injury.
Abstract The transcription factor NRF2, governed by its repressor KEAP1, protects cells against oxidative stress. There is interest in modelling the NRF2 response to improve the prediction of clinical toxicities such as drug-induced liver injury (DILI). However, very little is known about the makeup of the NRF2 transcriptional network and its response to chemical perturbation in primary human hepatocytes (PHH), which are often used as a translational model for investigating DILI. Here, microarray analysis identified 108 transcripts (including several putative novel NRF2-regulated genes) that were both downregulate...
Source: Archives of Toxicology - November 13, 2018 Category: Toxicology Authors: Copple IM, den Hollander W, Callegaro G, Mutter FE, Maggs JL, Schofield AL, Rainbow L, Fang Y, Sutherland JJ, Ellis EC, Ingelman-Sundberg M, Fenwick SW, Goldring CE, van de Water B, Stevens JL, Park BK Tags: Arch Toxicol Source Type: research

ARHGEF10L Contributes to Liver Tumorigenesis through RhoA-ROCK1 Signaling and the Epithelial-Mesenchymal Transition.
This study used the Illumina GoldenGate microassay, Sequenom MassARRAY and TaqMan to analyze possible correlations between tag single nucleotide polymorphisms (tag SNPs) in the ARHGEF10L locus and various tumor risks. The genotyping analyses demonstrated a strong association of rs2244444 and rs12732894 with liver cancer. Western blotting and immunohistochemistry also revealed increased expression of ARHGEF10L in hepatocellular carcinoma tissues. Furthermore, increased cell proliferation, cell migration and RhoA activity; increased expression of Rho-associated coiled-coil kinase-1 (ROCK1), phospho- Ezrin/Radixin/Moesin (ERM...
Source: Experimental Cell Research - November 13, 2018 Category: Cytology Authors: Tang J, Liu C, Xu B, Wang D, Ma Z, Chang X Tags: Exp Cell Res Source Type: research

Overexpression of Forkhead box C1 attenuates oxidative stress, inflammation and apoptosis in chronic obstructive pulmonary disease
Publication date: Available online 11 November 2018Source: Life SciencesAuthor(s): Shuyue Xia, Jian Qu, Hui Jia, Wei He, Jing Li, Long Zhao, Mingqing Mao, Yan ZhaoAbstractAimChronic obstructive pulmonary disease (COPD) is a disease caused by cigarette smoke, which has been emerging as a serious health problem worldwide. The aim of this study is to explore the mRNA expression profile of lung tissues from the COPD rats and to characterize the role of Forkhead box C1 (Foxc1) in COPD.Main methodsWistar rats were exposed to cigarette smoke during 16 weeks for COPD model establishment. The microarray was used to identify the d...
Source: Life Sciences - November 12, 2018 Category: Biology Source Type: research

Exploiting novel tailored immunotherapies of type 1 diabetes: Short interfering RNA delivered by cationic liposomes enables efficient Down-regulation of variant PTPN22 gene in T lymphocytes
Publication date: Available online 12 November 2018Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Marsha Pellegrino, Francesca Ceccacci, Stefania Petrini, Marco Cappa, Giovanna Mancini, Alessandra FierabracciAbstractIn autoimmune diseases as Type 1 diabetes, the actual treatment that provides the missing hormones is not able, however, to interrupt the underlining immunological mechanism. Importantly, novel immunotherapies are exploited to protect and rescue the remaining hormone producing cells. Among probable targets of immunotherapy, the C1858T mutation in the PTPN22 gene, which encodes for the lymp...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - November 12, 2018 Category: Nanotechnology Source Type: research

Carbohydrate response element binding protein (ChREBP) modulates the inflammatory response of mesangial cells in response to glucose.
Abstract Diabetic nephropathy (DN) is one of the most devastating complications of diabetes mellitus. Carbohydrate response element binding protein (ChREBP) is a basic helix-loop-helix leucine zipper transcription factor that primarily mediates glucose homeostasis in the body. The present study investigated the role of ChREBP in the pathogenesis of DN. The expression of ChREBP was detected in patients with type 2 diabetes mellitus (T2DM), diabetic mice and mesangial cells. ELISA was used to measure cytokine production in mesangial cells. Flow cytometry analysis was performed to detect the apoptosis of mes...
Source: Bioscience Reports - November 12, 2018 Category: Biomedical Science Authors: Chen Y, Wang YJ, Zhao Y, Wang JC Tags: Biosci Rep Source Type: research

Efficiency of dual siRNA mediated gene therapy for intervertebral disc degeneration (IVDD)
One of the common causes of LBP is intervertebral disc degeneration. The pathophysiology of disc degeneration involves apoptosis of nucleus pulposes (NP) cells and degradation of extra cellular matrix (ECM). Caspase 3 plays a central role in apoptosis and the ADAMTS 5 (A Disintegrin and Metalloproteinase with Thrombospondin motifs) gene plays a critical role in ECM degradation. Hence, we hypothesized that if one can silence these two genes, both apoptosis and ECM degradation can be prevented, thereby preventing the progression and even reverse disc degeneration.
Source: The Spine Journal - November 12, 2018 Category: Orthopaedics Authors: Banala Rajkiran Reddy, Vemuri Satish Kumar, Dar Ghulam Hassan, Palanisamy Vijayanand, P Murahari, MV Surekha, AV Gurava Reddy, Nalam Madhusudhana Rao, GPV Subbaiah Tags: Basic Science Source Type: research

The PKC β‐p66shc‐NADPH oxidase pathway plays a crucial role in diabetic nephropathy
ConclusionsThis study demonstrated enzastaurin alleviated diabetic renal injury via modulation of the PKC β‐p66shc‐NADPH oxidase pathway, which provided a new perspective for the treatment of early DN.
Source: Journal of Pharmacy and Pharmacology - November 12, 2018 Category: Drugs & Pharmacology Authors: Yu ‐Si Cheng, Jie Chao, Chen Chen, Lin‐Li Lv, Yu‐Chen Han, Bi‐Cheng Liu Tags: Research Paper Source Type: research

The absence of Rab27a accelerates the degradation of Melanophilin
This article is protected by copyright. All rights reserved.
Source: Experimental Dermatology - November 12, 2018 Category: Dermatology Authors: Jong il Park, Ji Eun Lee, Cheol hwan Myung, Chan song Jo, Hye Sung Jang, Jae Sung Hwang Tags: Methods Letter to the Editors Source Type: research

Efficiency of dual siRNA-mediated gene therapy for intervertebral disc degeneration (IVDD)
One of the common causes of low back pain is intervertebral disc degeneration. The pathophysiology of disc degeneration involves apoptosis of nucleus pulposes cells and degradation of extra cellular matrix (ECM). Caspase 3 plays a central role in apoptosis and the ADAMTS5 (A Disintegrin and Metalloproteinase with Thrombospondin motifs 5) gene plays a critical role in ECM degradation. Hence, we hypothesized that if one can silence these two genes, both apoptosis and ECM degradation can be prevented, thereby preventing the progression and even reverse disc degeneration.
Source: The Spine Journal - November 12, 2018 Category: Orthopaedics Authors: Rajkiran Reddy Banala, Satish Kumar Vemuri, Ghulam Hassan Dar, Vijayanand Palanisamy, Murahari P, Surekha MV, Gurava Reddy AV, Madhusudhana Rao Nalam, Subbaiah GPV Tags: Basic Science Source Type: research

Thymineless Death by the Fluoropyrimidine Polymer F10 Involves Replication Fork Collapse and Is Enhanced by Chk1 Inhibition.
Abstract We are developing the fluoropyrimidine polymer F10 to overcome limitations of 5-fluorouracil (5-FU) that result from inefficient metabolism to 5-fluoro-2'-deoxyuridine-5'-mono- and tri-phosphate, the deoxyribonucleotide metabolites that are responsible for 5-FU's anticancer activity. F10 is much more cytotoxic than 5-FU to colorectal cancer (CRC) cells; however, the mechanism of enhanced F10 cytotoxicity remains incompletely characterized. Using DNA fiber analysis, we establish that F10 decreases replication fork velocity and causes replication fork collapse, while 1000-fold excess of 5-FU is required to ...
Source: Neoplasia - November 12, 2018 Category: Cancer & Oncology Authors: Mani C, Pai S, Papke CM, Palle K, Gmeiner WH Tags: Neoplasia Source Type: research

MicroRNA-499-5p regulates skeletal myofiber specification via NFATc1/MEF2C pathway and Thrap1/MEF2C axis
This study aimed to investigate the role of microRNA-499-5p (miR-499-5p) in the regulation of skeletal myofiber specification and its underlying mechanisms.Main methodsMouse C2C12 cells were used in this study. Cyclosporin A and siRNA targeting Thrap1 (si-Thrap1) were used to inhibit NFATc1/MEF2C pathway and knockdown Thrap1, respectively. The expressions of miR-499-5p and genes were evaluated by real-time quantitative PCR and western blot analysis.Key findingsOverexpression of miR-499-5p promoted oxidative fiber gene expression and repressed glycolytic fiber gene expression, affecting several factors associated with fiber...
Source: Life Sciences - November 11, 2018 Category: Biology Source Type: research

Silencing of Xeroderma Pigmentosum Group D Gene Promotes Hepatoma Cell Growth by Reducing P53 Expression.
CONCLUSIONS Our data revealed that XPD silencing promoted growth of hepatoma cells by reducing P53 expression. PMID: 30409962 [PubMed - in process]
Source: Medical Science Monitor - November 11, 2018 Category: Research Tags: Med Sci Monit Source Type: research

Gastric Cancer Cell Lines Have Different MYC-Regulated Expression Patterns but Share a Common Core of Altered Genes.
Authors: Maués JHDS, Ribeiro HF, Pinto GR, Lopes LO, Lamarão LM, Pessoa CMF, Moreira-Nunes CFA, de Carvalho RM, Assumpção PP, Rey JA, Burbano RMR Abstract MYC is an oncogene responsible for excessive cell growth in cancer, enabling transcriptional activation of genes involved in cell cycle regulation, metabolism, and apoptosis, and is usually overexpressed in gastric cancer (GC). By using siRNA and Next-Generation Sequencing (NGS), we identified MYC-regulated differentially expressed Genes (DEGs) in three Brazilian gastric cancer cell lines representing the histological subtypes of GC (diffuse, intestinal, and ...
Source: Canadian Journal of Gastroenterology - November 11, 2018 Category: Gastroenterology Tags: Can J Gastroenterol Hepatol Source Type: research

Erythropoietin reduces nerve demyelination, neuropathic pain behavior and microglial MAPKs activation through erythropoietin receptors on Schwann cells in a rat model of peripheral neuropathy
In conclusion, binding of EPO to its receptors on Schwann cells maintains myelin integrity and blocks ectop ic discharges in the injured median nerve, that in the end contribute to attenuation of neuropathic pain via reducing glutamate release from primary afferents and inhibiting activation of microglial MAPKs and production of pro‐inflammatory cytokines.
Source: Glia - November 11, 2018 Category: Neurology Authors: Chun ‐Ta Huang, Seu‐Hwa Chen, Shih‐Chang Lin, Wei‐Ting Chen, June‐Horng Lue, Yi‐Ju Tsai Tags: Research Article Source Type: research

Cancer stem cells (CSCs) in cancer progression and therapy
Most of the tumors are occupied with a number of self ‐renewing cells called cancer stem cells (CSCs) that are contributed to the initiation, maintenance, and thriving cancer. The cells have rather similar characteristics to other stem cells located in the niche of body organs, but they have not essentially the same responses to the diverse stimuli. There is evidence for repopulation of CSCs after treatment with chemo/radiotherapy, which is possibly because of their highly plastic feature. Normal stem cells have the proclivity to transform into CSCs when they undergo continuous mutagenesis or receive tumorigenic signals ...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Masoud Najafi, Bagher Farhood, Keywan Mortezaee Tags: REVIEW ARTICLE Source Type: research

Licochalcone A attenuates abdominal aortic aneurysm induced by angiotensin II via regulating the miR ‐181b/SIRT1/HO‐1 signaling
This study aimed to investigate the role and underlying mechanism of LA in the abdominal aortic aneurysm (AAA). AAA model was established by continuous infusion of 1000 ng/kg/min of angiotensin II (AngII) in ApoE‐/‐ mice for 4 weeks. At 7 days before AngII administration, 5  mg/kg/day or 10 mg/kg/day of LA was intraperitoneally administered to mice and continued for 4 weeks. The characteristics and quantification of AAAs were determined in situ. Real‐time PCR or western blot was used to measure mRNA or protein levels of matrix metalloproteinase 2 and matrix metal loproteinase 9; pro‐inflammatory cytokines tum...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Xuhui Hou, Songbai Yang, Yan Zheng Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Effects of microRNA ‐217 on proliferation, apoptosis, and autophagy of hepatocytes in rat models of CCL4‐induced liver injury by targeting NAT2
This study aims to explore the effects of miR ‐217 targeting NAT2 on hepatocyte proliferation, apoptosis, and autophagy following carbon tetrachloride (CCL4)‐induced liver injury. Rat models of CCL4‐induced liver injury were established. Healthy Wistar rats were randomized into the normal, blank, negative control (NC), microRNA‐217 (miR ‐217) mimic, miR‐217 inhibitor, small interfering RNA (siRNA)‐N‐acetyltransferase 2 (NAT2), and miR‐217 inhibitor + siRNA‐NAT2 groups. NAT2 activity was evaluated with reversed‐phase high‐performance liquid chromatographic method. Immunohistochemistry was used t...
Source: Journal of Cellular Physiology - November 11, 2018 Category: Cytology Authors: Cheng ‐Liang Yang, Xiao‐Li Zheng, Ke Ye, Ya‐Nan Sun, Yu‐Fei Lu, Hong Ge, Hui Liu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Acidosis promotes cell apoptosis through the G protein-coupled receptor 4/CCAAT/enhancer-binding protein homologous protein pathway.
Authors: Dong B, Zhang X, Fan Y, Cao S, Zhang X Abstract The aim of the present study was to investigate the effects of acidosis on the apoptosis of renal epithelial and endothelial cells, and the molecular pathways responsible for this. A human proximal tubular cell line, HK-2, and human umbilical vein endothelial cells (HUVECs), were transfected with control or G protein-coupled receptor 4 siRNA for 36 h. Cells were exposed to normal (pH 7.4) or acidic (pH 6.4) media. Western blot analysis was used to assess the protein expression levels of G protein-coupled receptor 4 (GPR4), CCAAT/enhancer-binding protein homol...
Source: Oncology Letters - November 9, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Knockdown of LETM1 inhibits proliferation and metastasis of human renal cell carcinoma cells.
In conclusion, these results suggest that knockdown of LETM1 exhibits tumor suppressive effects, at least in part by controlling the downstream Wnt/β-Catenin signaling pathway. Therefore, LETM1 may act as a novel therapeutic target for the treatment of RCC. PMID: 30405774 [PubMed]
Source: Oncology Letters - November 9, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Effects of PLK1 on proliferation, invasion and metastasis of gastric cancer cells through epithelial-mesenchymal transition.
In conclusion, PLK1 enhances the proliferation, migration and invasion of gastric cancer MGC-803 cells through affecting EMT. PMID: 30405751 [PubMed]
Source: Oncology Letters - November 9, 2018 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

Impact of GADD34 on Apoptosis of Tonsillar Mononuclear Cells from IgA Nephropathy Patients by Regulating Eif2 α Phosphorylation
Conclusion: GADD34 may be a potential therapeutic target for IgAN treatment due to its effect on cell apoptosis.Cell Physiol Biochem 2018;50:2203 –2215
Source: Cellular Physiology and Biochemistry - November 9, 2018 Category: Cytology Source Type: research

MicroRNA-323a-3p Promotes Pressure Overload-Induced Cardiac Fibrosis by Targeting TIMP3
Conclusion: Our findings provide evidence that miR-323a-3p promotes cardiac fibrosis via miR-323a-3p-TIMP3-TGF- β pathway. miR-323a-3p may be a new marker for cardiac fibrosis progression and that inhibition of miR-323a-3p may be a promising therapeutic target for the treatment of cardiac fibrosis.Cell Physiol Biochem 2018;50:2176 –2187
Source: Cellular Physiology and Biochemistry - November 9, 2018 Category: Cytology Source Type: research

Genome-wide association analysis identifies SNPs predictive of in vitro leukemic cell sensitivity to cytarabine in pediatric AML.
Authors: Bargal SA, Rafiee R, Crews KR, Wu H, Cao X, Rubnitz JE, Ribeiro RC, Downing JR, Pounds SB, Lamba JK Abstract Cytarabine has been an integral part of acute myeloid leukemia (AML) chemotherapy for over four decades. However, development of resistance and high rates of relapse is a significant impediment in successfully treating AML. We performed a genome-wide association analysis (GWAS) and identified 113 (83 after adjusting for Linkage Disequilibrium) SNPs associated with in vitro cytarabine chemosensitivity of diagnostic leukemic cells from a cohort of 50 pediatric AML patients (p<10-4). Further evaluat...
Source: Oncotarget - November 9, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

The Application of Nanoparticle-Based Drug Delivery Systems in Checkpoint Blockade Cancer Immunotherapy.
In conclusion, the combination nanoparticle with checkpoint blockade cancer immunity is a promising direction that may fulfill the requirement of cancer treatment. PMID: 30406152 [PubMed - in process]
Source: Journal of Immunology Research - November 9, 2018 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

The novel exchange protein activated by cyclic AMP 1 (EPAC1) agonist, I942, regulates inflammatory gene expression in human umbilical vascular endothelial cells (HUVECs)
Publication date: Available online 9 November 2018Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Jolanta Wiejak, Boy van Basten, Urszula Luchowska-Stańska, Graham Hamilton, Stephen J. YarwoodAbstractExchange protein activated by cyclic AMP (EPAC1) suppresses multiple inflammatory actions in vascular endothelial cells (VECs), partly due to its ability to induce expression of the suppressor of cytokine signalling 3 (SOCS3) gene, the protein product of which inhibits interleukin 6 (IL6) signalling through the JAK/STAT3 pathway. Here, for the first time, we use the non-cyclic nucleotide EPAC1 ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 9, 2018 Category: Molecular Biology Source Type: research

ERK and miRNA-1 target Cx43 expression and phosphorylation to modulate the vascular protective effect of angiotensin II
In this study, we investigated whether the beneficial effects of AngII are related to regulation of gap junctions (GJs) and connexin43 (Cx43), and the implication of MAPK signaling and microRNA (miR-1) in this process.Key findingsOur results show that after hemorrhagic shock or hypoxia, the blockade of GJs or knockdown of Cx43 inhibits the AngII-induced increase in vascular reactivity of superior mesenteric arteries and the contractile response of vascular smooth muscle cells (VSMCs). AngII treatment increases Cx43 expression and phosphorylation at Ser262, and restores gap-junctional communication (GJIC) between VSMCs afte...
Source: Life Sciences - November 9, 2018 Category: Biology Source Type: research

Melatonin antagonizes oxidative stress-induced mitochondrial dysfunction in retinal pigmented epithelium cells via melatonin receptor 1 (MT1).
Authors: Yan G, Yu L, Jiang S, Zhu J Abstract High energy-consumption in retinal pigmented epithelium (RPE) cells poses oxidative stress (OS) and contributes to mitochondrial dysfunction (MD) for retinal degeneration-associated diseases. In the present study, we evaluated the protective role of Melatonin, a natural antioxidant, against the hydrogen peroxide (H2O2)-induced damage to RPE cells. The cellular viability, apoptosis, the expression of apoptosis-associated proteins and mitochondrial function were examined in the retinal ARPE-19 cells, post the treatment with H2O2 or (and) with Melatonin. The regulation by ...
Source: Journal of Toxicological Sciences - November 9, 2018 Category: Toxicology Tags: J Toxicol Sci Source Type: research

Hypoxia enhances the migration and invasion of human glioblastoma U87 cells through PI3K/Akt/mTOR/HIF-1α pathway
This study aims to explore the role of phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway and its relationship with hypoxia inducible factor-1α (HIF-1α) in the migration and invasion of human glioblastoma U87 cells under hypoxia. In the study, we found that hypoxia could activate the PI3K/Akt/mTOR pathway associated with the enhancements of the migration and invasion of human glioblastoma U87 cells. When the PI3K/Akt/mTOR pathway and HIF-1α were inhibited by the siRNAs or inhibitors, the migration and invasion of human glioblastoma U87 cells were suppressed. Meanwhile, t...
Source: NeuroReport - November 9, 2018 Category: Neurology Tags: CELLULAR, MOLECULAR AND DEVELOPMENTAL NEUROSCIENCE Source Type: research

Triclosan Suppresses Testicular Steroidogenesis via the miR-6321/JNK/ Nur77 Cascade
Conclusion: Taken together, the miR-6321/Map3k1-regulated JNK/c-Jun/ Nur77 cascade contributes to TCS-caused suppression of testicular steroidogenesis, and the decrease in 5 α-Reductase expressions may be the compensatory mechanism.Cell Physiol Biochem 2018;50:2029 –2045
Source: Cellular Physiology and Biochemistry - November 9, 2018 Category: Cytology Source Type: research

Homocysteine induced oxidative stress in human umbilical vein endothelial cells via regulating methylation of SORBS1.
CONCLUSIONS: We indicated that Hcy induced oxidative stress in HUVECs via regulating methylation of SORBS1. We also found that Fc and B12 treatment attenuated the oxidative stress and inflammation induced by Hcy in HUVECs. The findings indicated that Fc and B12 might be effective agents for the treatment of Hcy induced AS. PMID: 30402861 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - November 9, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Sirtuin 6 promotes cell aging of myeloma cell line KM-HM_(31) by via Hippo signal pathway.
CONCLUSIONS: We showed that sirtuin 6 facilitates CP-induced myeloma cell KM-HM_(31) aging via suppressing Hippo. PMID: 30402853 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - November 9, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

MiR-126 induces myeloma cell line Karpas707 apoptosis by downregulating anti-apoptotic protein MCL.
CONCLUSIONS: MiR-126 induces Karpas707 cell apoptosis by downregulating anti-apoptotic protein MCL, which provides a theoretical basis for the target selection of myeloma. PMID: 30402852 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - November 9, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

HIF-1 α restricts proliferation and apoptosis of Tca8113 cells through up regulation of Hippo signaling pathway under hypoxic conditions.
CONCLUSIONS: Under hypoxic conditions, HIF-1α inhibits the proliferation and apoptosis of Tca8113 cells via the elevation of the Hippo signaling pathway. PMID: 30402847 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - November 9, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Effect of hypoxia on the Twist1 in EMT of cervical cancer cells.
CONCLUSIONS: Hypoxia may account for the increased invasion of HeLa cells, which is realized by the up-regulated GLI-1. PMID: 30402835 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - November 9, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

The involvement of miR-155 in blood pressure regulation in pregnant hypertension rat via targeting FOXO3a.
CONCLUSIONS: The inhibition of miR-155 improves the damage of pregnant hypertension via the upregulation of FOXO3a, which provides academic leads for the future therapy of pregnant hypertension. PMID: 30402830 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - November 9, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Kinome-Wide siRNA Screening Identifies Src-Enhanced Resistance of Chemotherapeutic Drugs in Triple-Negative Breast Cancer Cells
Yen-Dun Tony Tzeng, Pei-Feng Liu, Ju-Yueh Li, Li-Feng Liu, Soong-Yu Kuo, Chiao-Wei Hsieh, Cheng-Hsin Lee, Chih-Hsuan Wu, Michael Hsiao, Hong-Tai Chang, Chih-Wen Shu
Source: Frontiers in Pharmacology - November 9, 2018 Category: Drugs & Pharmacology Source Type: research

Uterine RGS2 expression is regulated by exogenous estrogen and progesterone in ovariectomized mice, and down ‐regulation of RGS2 expression in artificial decidualized ESCs inhibits trophoblast spreading in vitro
This article is protected by copyright. All rights reserved.
Source: Molecular Reproduction and Development - November 9, 2018 Category: Reproduction Medicine Authors: Manxi Jiang, Liangshan Hu, Baoping Wang, Danxia Chen, Yahong Li, Zhen Zhang, Yan Zhu Tags: RESEARCH ARTICLE Source Type: research

β‑glucan, a dectin‑1 ligand, promotes macrophage M1 polarization via NF‑κB/autophagy pathway.
Abstract Pro‑inflammatory (M1) macrophages have key roles in atherogenesis. As β‑glucan has been demonstrated to exert pro‑inflammatory effects, the present study examined whether β‑glucan exerts atherogenic effects via converting macrophages into M1 phenotype. The results from reverse transcription‑quantitative polymerase chain reaction, flow cytometry, western blotting and transmission electron microscope indicated that M1 macrophage markers inducible nitric oxide synthase and cluster of differentiation 80 were upregulated, dectin‑1 (a receptor for β‑glucan) expression and nuclear factor (NF)...
Source: International Journal of Oncology - November 9, 2018 Category: Cancer & Oncology Authors: Li X, Luo H, Ye Y, Chen X, Zou Y, Duan J, Xiang D Tags: Int J Oncol Source Type: research

ROS-induced HSP70 promotes cytoplasmic translocation of high-mobility group box 1b and stimulates antiviral autophagy in grass carp kidney cells Cell Biology
In this study, we found that both grass carp reovirus (GCRV) infection and hydrogen peroxide (H2O2) treatment induced the accumulation of reactive oxygen species (ROS) in C. idella kidney cells and stimulate autophagy. Suppressing ROS accumulation with N-acetyl-l-cysteine significantly inhibited GCRV-induced autophagy activation and enhanced GCRV replication. Although ROS-induced autophagy, in turn, restricted GCRV replication, further investigation revealed that the multifunctional cellular protein high-mobility group box 1b (HMGB1b) serves as a heat shock protein 70 (HSP70)–dependent, pro-autophagic protein in grass ca...
Source: Journal of Biological Chemistry - November 9, 2018 Category: Chemistry Authors: Youliang Rao, Quanyuan Wan, Hang Su, Xun Xiao, Zhiwei Liao, Jianfei Ji, Chunrong Yang, Li Lin, Jianguo Su Tags: Immunology Source Type: research

Bushenhuoxue Formula Facilitates Articular Cartilage Repair and Attenuates Matrix Degradation by Activation of TGF- β Signaling Pathway.
Conclusion: By activation of TGF-β signaling, BSHX formula can promote articular cartilage repair by accelerating chondrocyte proliferation and maintaining chondrocyte phenotype, upregulate ECM accumulation, and inhibit matrix degradation. PMID: 30402118 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - November 8, 2018 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

γ‐secretase inhibitor DAPT mitigates cisplatin‐induced acute kidney injury by suppressing Notch1 signaling
AbstractOrgan toxicity, including kidney injury, limits the use of cisplatin for the treatment of multiple human cancers. Hence, interventions to alleviate cisplatin ‐induced nephropathy are of benefit to cancer patients. Recent studies have demonstrated that pharmacological inhibition of the Notch signaling pathway enhances cisplatin efficacy against several cancer cells. However, whether augmentation of the anti‐cancer effect of cisplatin by Notch inhibiti on comes at the cost of increased kidney injury is unclear. We show here that treatment of mice with cisplatin resulted in a significant increase in Notch ligand D...
Source: Journal of Cellular and Molecular Medicine - November 8, 2018 Category: Molecular Biology Authors: Hitesh Soni, Anberitha T. Matthews, Sandeep Pallikkuth, Rajashekhar Gangaraju, Adebowale Adebiyi Tags: ORIGINAL ARTICLE Source Type: research

The roles of hypoxia-inducible Factor-1 and iron regulatory protein 1 in iron uptake induced by acute hypoxia.
CONCLUSIONS: We propose that HIF-1/HRE system might play a principal part in hypoxia induced iron uptake. PMID: 30415773 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - November 8, 2018 Category: Biochemistry Authors: Yang L, Wang D, Wang XT, Lu YP, Zhu L Tags: Biochem Biophys Res Commun Source Type: research

γ-secretase inhibitor DAPT mitigates cisplatin-induced acute kidney injury by suppressing Notch1 signaling.
Abstract Organ toxicity, including kidney injury, limits the use of cisplatin for the treatment of multiple human cancers. Hence, interventions to alleviate cisplatin-induced nephropathy are of benefit to cancer patients. Recent studies have demonstrated that pharmacological inhibition of the Notch signaling pathway enhances cisplatin efficacy against several cancer cells. However, whether augmentation of the anti-cancer effect of cisplatin by Notch inhibition comes at the cost of increased kidney injury is unclear. We show here that treatment of mice with cisplatin resulted in a significant increase in Notch liga...
Source: J Cell Mol Med - November 8, 2018 Category: Molecular Biology Authors: Soni H, Matthews AT, Pallikkuth S, Gangaraju R, Adebiyi A Tags: J Cell Mol Med Source Type: research

Caveolin-1 Modulates Mechanotransduction Responses to Substrate Stiffness through Actin-Dependent Control of YAP
Publication date: 6 November 2018Source: Cell Reports, Volume 25, Issue 6Author(s): Roberto Moreno-Vicente, Dácil María Pavón, Inés Martín-Padura, Mauro Català-Montoro, Alberto Díez-Sánchez, Antonio Quílez-Álvarez, Juan Antonio López, Miguel Sánchez-Álvarez, Jesús Vázquez, Raffaele Strippoli, Miguel A. del PozoSummaryThe transcriptional regulator YAP orchestrates many cellular functions, including tissue homeostasis, organ growth control, and tumorigenesis. Mechanical stimuli are a key input to YAP activity, but the mechanisms controlling this regulation remain largely uncharacterized. We show that CAV1 posi...
Source: Cell Reports - November 7, 2018 Category: Cytology Source Type: research