Am J Physiol Cell Physiol; +41 new citations
41 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These pubmed results were generated on 2020/08/04PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - August 5, 2020 Category: Cytology Tags: Report Source Type: research

Am J Physiol Cell Physiol; +27 new citations
27 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These pubmed results were generated on 2020/07/31PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - July 31, 2020 Category: Cytology Tags: Report Source Type: research

Regulation of Lin28a-miRNA let-7b-5p pathway in the skeletal muscle cells by PPAR δ.
Regulation of Lin28a-miRNA let-7b-5p pathway in the skeletal muscle cells by PPARδ. Am J Physiol Cell Physiol. 2020 Jul 22;: Authors: Araujo HN, Lima TI, Guimarães DSPSF, Oliveira AG, Favero-Santos BC, Branco RCS, Araújo RMDS, Dantas AFB, Castro A, Chacon-Mikahill MPT, Minatel E, Geraldo MV, Carneiro EM, Rodrigues AC, Narkar VA, Silveira LR Abstract Lin28a/miRNA let-7b-5p pathway has emerged as a key regulators of energy homeostasis in the skeletal muscle. However, the mechanism through which this pathway is regulated in the skeletal muscle has remained unclear. We have found that ...
Source: Am J Physiol Cell Ph... - July 22, 2020 Category: Cytology Authors: Araujo HN, Lima TI, Guimarães DSPSF, Oliveira AG, Favero-Santos BC, Branco RCS, Araújo RMDS, Dantas AFB, Castro A, Chacon-Mikahill MPT, Minatel E, Geraldo MV, Carneiro EM, Rodrigues AC, Narkar VA, Silveira LR Tags: Am J Physiol Cell Physiol Source Type: research

Microfluidic confinement enhances phenotype and function of hepatocyte spheroids.
Abstract A number of cell culture approaches have been described for maintenance of primary hepatocytes. Forming hepatocytes into 3D spheroids is one well accepted method for extending epithelial phenotype of these cells. Our lab has previously observed enhanced function of 2D (monolayer) hepatocyte cultures in microfluidic devices due to increased production of several hepato-inductive growth factors, including hepatocyte growth factor (HGF). In the present study we wanted to test a hypothesis that placing hepatocytes spheroids (3D) into microfluidic devices will also result in enhanced phenotype and function. To...
Source: Am J Physiol Cell Ph... - July 22, 2020 Category: Cytology Authors: Choi JH, Loarca L, De Hoyos-Vega JM, Dadgar N, Loutherback K, Shah VH, Stybayeva G, Revzin A Tags: Am J Physiol Cell Physiol Source Type: research

Inhibiting Fusion with Cellular Membrane System: Therapeutic Options to Prevent Severe Acute Respiratory Syndrome Coronavirus-2 Infection.
Abstract Severe acute respiratory syndrome coronavirus (SARS-CoV), an enveloped virus with a positive-sense single-stranded RNA genome facilitates the host cell entry through intricate interactions with proteins and lipids of the cell membrane. The detailed molecular mechanism involves binding to the host cell receptor and fusion at the plasma membrane or after being trafficked to late endosomes under favorable environmental conditions. A crucial event in the process is the proteolytic cleavage of the viral spike protein by the host's endogenous proteases that releases the fusion peptide enabling fusion with the h...
Source: Am J Physiol Cell Ph... - July 20, 2020 Category: Cytology Authors: Mitra P Tags: Am J Physiol Cell Physiol Source Type: research

Neutrophil heterogeneity and fate in inflamed tissues: implications for the resolution of inflammation.
Abstract Neutrophils are polymorphonuclear leukocytes that play a central role in host defense against infection and tissue injury. They are rapidly recruited to the inflamed site and execute a variety of functions to clear invading pathogens and damaged cells. However, many of their defense mechanisms are capable of inflicting collateral tissue damage. Neutrophil-driven inflammation is a unifying mechanism underlying many common diseases. Efficient removal of neutrophils from inflammatory loci is critical for timely resolution of inflammation and return to homeostasis. Accumulating evidence challenges the classic...
Source: Am J Physiol Cell Ph... - July 15, 2020 Category: Cytology Authors: Filep JG, Ariel A Tags: Am J Physiol Cell Physiol Source Type: research

Let-7d-5p suppresses inflammatory response in neonatal rats with necrotizing enterocolitis via LGALS3-mediated TLR4/NF- κB signaling pathway.
This study aimed to identify the possible roles of let-7d-5p in neonatal rats with NEC. The differentially expressed genes (DEGs) related to NEC were initially screened in silico. After establishment of NEC rat models, the measurement of the expression of let-7d-5p, galectin-3 (LGALS3), toll-like receptor 4 (TLR4) and nuclear factor kappa B (NF-κB) as well as proinflammatory cytokines (TNF-α, IL-1b and IL-6) was conducted. The interaction between let-7d-5p and LGALS3 or Argonaute2 (AGO2) was identified. Gain- and loss-function approaches were then performed in an attempt to investigate the regulatory roles of l...
Source: Am J Physiol Cell Ph... - July 15, 2020 Category: Cytology Authors: Sun L, Sun M, Ma K, Liu J Tags: Am J Physiol Cell Physiol Source Type: research

The Role of Lamina Cribrosa Tissue Stiffness and Fibrosis as Fundamental Biomechanical Drivers of Pathological Glaucomatous Cupping.
Abstract The primary biomechanical driver of pathological glaucomatous cupping remains unknown. Finite element modelling indicates that stress and strain play key roles. Primarily a review article we utilise known biomechanical data and currently unpublished results from our lab to propose a 3 stage, tissue stiffness-based model to explain glaucomatous cupping occurring at variable levels of translaminar pressure (TLP). In stage 1, a short-term increase in TLP gradient induces a transient increase in LC strain. Beyond a critical level of strain, the tissue stiffness rises steeply provoking cellular responses via i...
Source: Am J Physiol Cell Ph... - July 15, 2020 Category: Cytology Authors: Hopkins AA, Murphy R, Irnaten M, Wallace DM, Quill B, 'Brien C Tags: Am J Physiol Cell Physiol Source Type: research

Resolution of inflammation in immune and non-immune cells by Interleukin-19.
Abstract The inflammatory response is a complex, tightly regulated process activated by tissue wounding, foreign body invasion, and sterile inflammation. Over the decades, great progress has been made to advance our understanding of this process. One often overlooked aspect of inflammation is its sequel; resolution. We know that dysregulated resolution often results in numerous chronic degenerative diseases such as arthritis, cancer, and asthma. However, identification of components and mechanisms of resolving pathways lag behind those of pro-inflammatory processes, yet represent overlooked therapeutic opportuniti...
Source: Am J Physiol Cell Ph... - July 15, 2020 Category: Cytology Authors: Leigh T, Scalia RG, Autieri MV Tags: Am J Physiol Cell Physiol Source Type: research

AJP-Cell Physiology begins a Theme of Reviews on "New Frontiers in Protein Trafficking".
AJP-Cell Physiology begins a Theme of Reviews on "New Frontiers in Protein Trafficking". Am J Physiol Cell Physiol. 2020 Jul 15;: Authors: Hamilton KL PMID: 32667868 [PubMed - as supplied by publisher] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - July 15, 2020 Category: Cytology Authors: Hamilton KL Tags: Am J Physiol Cell Physiol Source Type: research

Mini-review: Recent Progress on Surfactant Protein-A Cellular Function in Lung and Kidney Disease Development.
Abstract Pulmonary surfactant is a heterogeneous active surface complex made up of lipids and proteins. The major glycoprotein in surfactant is surfactant protein A (SP-A), which is released into the alveolar lumen from cytoplasmic lamellar bodies in type II alveolar epithelial cells. SP-A is involved in phospholipid absorption. SP-A together with other surfactant proteins and phospholipids prevent alveolar collapse during respiration by decreasing the surface tension of the air/liquid interface. Additionally, SP-A interacts with pathogens to prevent their propagation and regulate host immune responses. Studies in...
Source: Am J Physiol Cell Ph... - July 8, 2020 Category: Cytology Authors: King SD, Chen SY Tags: Am J Physiol Cell Physiol Source Type: research

Dual-Specificity Phosphatase 29 is Induced During Neurogenic Skeletal Muscle Atrophy and Attenuates Glucocorticoid Receptor Activity in Muscle Cell Culture.
Abstract Skeletal muscle atrophy is caused by a decrease in muscle size and strength and results from a range of physiological conditions, including denervation, immobilization, corticosteroid exposure and aging. Newly named Dual Specificity Phosphatase 29 (Dusp29) has been identified as a novel neurogenic atrophy-induced gene in skeletal muscle. qPCR analysis revealed that Dusp29 expression is significantly higher in differentiated myotubes compared proliferating myoblasts. To determine how Dusp29 is transcriptionally regulated in skeletal muscle, fragments of the promoter region of Dusp29 were cloned, fused to a...
Source: Am J Physiol Cell Ph... - July 8, 2020 Category: Cytology Authors: Cooper LM, West RC, Hayes CS, Waddell DS Tags: Am J Physiol Cell Physiol Source Type: research

Bioprinting stem cells: building physiological tissues one cell at a time.
Abstract Bioprinting aims to direct the spatial arrangement in three dimensions of cells, biomaterials and growth factors. The biofabrication of clinically relevant constructs for the repair or modelling of either diseased or damaged tissues is rapidly advancing, resulting in the ability to 3D print biomimetic platforms which imitate a large number of tissues in the human body. Primary tissue-specific cells are typically isolated from patients and used for the fabrication of 3D models for drug screening or tissue repair purposes. However, the lack of resilience of these platforms, due to the difficulties in harnes...
Source: Am J Physiol Cell Ph... - July 8, 2020 Category: Cytology Authors: Scognamiglio C, Soloperto A, Ruocco G, Cidonio G Tags: Am J Physiol Cell Physiol Source Type: research

MARCKS-Like Protein-1(MLP-1) regulates ENaC Activity in Renal Distal Convoluted Tubule Cells.
We examined MLP-1 regulation of ENaC in DCT-15 cells. MLP-1 consists of a positively charged effector domain that sequesters PIP2 and contains serines that are a PKC targets and controls MLP-1 membrane association; a myristoylation domain promoting membrane association, and a multiple homology 2 domain of unknown function. We constructed several MLP-1 mutants: WT: a full length wild-type protein; S3A: 3 substitutions in the effector domain to prevent phosphorylation; S3D: replaced three serines with aspartates to mimic constitutive phosphorylation; GA: replaced the myristoylation site glycine with alanine so GA could not b...
Source: Am J Physiol Cell Ph... - July 8, 2020 Category: Cytology Authors: Song C, Yue Q, Moseley A, Al-Khalili O, Wynne BM, Ma H, Wang L, Eaton DC Tags: Am J Physiol Cell Physiol Source Type: research

Serum extracellular vesicle miR-203a-3p content is associated with skeletal muscle mass and protein turnover during disuse atrophy and regrowth.
Abstract Small non-coding microRNAs (miRNAs) are important regulators of skeletal muscle size and circulating miRNAs within extracellular vesicles (EVs) may contribute to atrophy and its associated systemic effects. The purpose of this study was to understand how muscle atrophy and regrowth alters in vivo serum EV miRNA content. We also associated changes in serum EV miRNA with protein synthesis, protein degradation and miRNA within muscle, kidney and liver. We subjected adult (10 mo.) F334/BN rats to 3 conditions: weight-bearing (WB), hindlimb suspension (HS) for 7 days to induce muscle atrophy, and HS for 7 days...
Source: Am J Physiol Cell Ph... - July 8, 2020 Category: Cytology Authors: Van Pelt DW, Vechetti IJ, Lawrence MM, Van Pelt KL, Patel P, Miller BF, Butterfield TA, Dupont-Versteegden EE Tags: Am J Physiol Cell Physiol Source Type: research

Upregulating microRNA-874-3p inhibits CXCL12 expression to promote angiogenesis and suppress inflammatory response in ischemic stroke.
Abstract Identification of specific biomarkers for ischemic stroke is necessary due to their abilities to improve treatment outcomes. Many studies have demonstrated the involvement of microRNAs (miRNAs) in the pathogenesis and complications of ischemic stroke and patient outcomes. We found that the expression of miR-874-3p was downregulated in clinical samples of ischemic stroke. Thus, the present study explored the potential role of miR-874-3p in ischemic stroke and related mechanisms. A mouse model of ischemic stroke was constructed by middle cerebral artery occlusion. The relationship among miR-874-3p, C-X-C mo...
Source: Am J Physiol Cell Ph... - July 1, 2020 Category: Cytology Authors: Xie K, Cai Y, Yang P, Du F, Wu K Tags: Am J Physiol Cell Physiol Source Type: research

MicroRNA-99b-5p downregulates protein synthesis in human primary myotubes.
Abstract MicroRNAs (miRNAs) are important regulators of cellular homeostasis and exert their effect by directly controlling protein expression. We have previously reported an age-dependent, negative association between microRNA-99b (miR-99b-5p) expression and muscle protein synthesis in human muscle in vivo. Here we investigated the role of miR-99b-5p as a potential negative regulator of protein synthesis via inhibition of MTOR signalling in human primary myocytes. Overexpressing miR-99b-5p in human primary myotubes from young and old subjects significantly decreased protein synthesis with no effect of donor age. ...
Source: Am J Physiol Cell Ph... - July 1, 2020 Category: Cytology Authors: Zacharewicz E, Kalanon M, Murphy RM, Russell AP, Lamon S Tags: Am J Physiol Cell Physiol Source Type: research

Extracellular Matrix Dynamics in Vascular Remodeling.
Abstract Vascular remodeling is the adaptive response to various physiological and pathophysiological alterations that are closely related to aging and vascular diseases. Understanding the mechanistic regulation of vascular remodeling may be favorable for discovering potential therapeutic targets and strategies. The extracellular matrix (ECM), including matrix proteins and their degradative metalloproteases, serves as the main component of the microenvironment and exhibits dynamic changes during vascular remodeling. This process mainly involves the altered composition of matrix proteins, metalloprotease-mediated d...
Source: Am J Physiol Cell Ph... - June 24, 2020 Category: Cytology Authors: Ma Z, Mao C, Jia Y, Fu Y, Kong W Tags: Am J Physiol Cell Physiol Source Type: research

WNK3 and WNK4 Exhibit Opposite Sensitivity with Respect to Cell Volume and Intracellular Chloride Concentration.
mba G Abstract Cation-coupled chloride cotransporters (CCC) play a role in modulating intracellular chloride concentration ([Cl-]i) and cell volume. Cell shrinkage and cell swelling are accompanied by an increase or decrease in [Cl-]i, respectively. Cell shrinkage and a decrease in [Cl-]i increases the activity of NKCCs (Na-K-Cl cotransporters: NKCC1, NKCC2 and Na-Cl) and inhibits the activity of KCCs (K-Cl cotransporters: KCC1 to KCC4), while cell swelling and an increase in [Cl-]i activates KCCs and inhibits NKCCs; thus, it is unlikely that the same kinase is responsible for both effects. WNK1 and WNK4 are chlor...
Source: Am J Physiol Cell Ph... - June 24, 2020 Category: Cytology Authors: Pacheco-Alvarez D, Carrillo-Perez DL, Mercado A, Leyva-Ríos K, Moreno E, Hernandez-Mercado E, Castaneda-Bueno M, Vazquez N, Gamba G Tags: Am J Physiol Cell Physiol Source Type: research

Active modulation of erythrocyte mechanics.
Abstract The classic view of the red blood cell (RBC) presents a biologically inert cell, that upon maturation, has limited capacity to alter its physical properties. This view developed largely due to the absence of translational machinery and inability to synthesise or repair proteins in circulating RBC. Recent developments have challenged this perspective, supporting the importance of post-translational modifications, and greater understanding of ion movement in these cells, that each regulate a myriad of cellular properties. There is thus now sufficient evidence to induce a step-change in understanding of RBC;...
Source: Am J Physiol Cell Ph... - June 24, 2020 Category: Cytology Authors: Kuck L, Peart JN, Simmonds MJ Tags: Am J Physiol Cell Physiol Source Type: research

Cholinergic-induced anion secretion in murine jejunal enteroids involves synergy between muscarinic and nicotinic pathways.
Abstract Acetylcholine induces robust electrogenic anion secretion in mammalian intestine and it has long been hypothesized that it mediates the epithelial response through the M3 and, to a lesser extent, the M1 muscarinic receptors in the mouse. However, nicotinic receptors have recently been identified in intestinal enterocytes by qRT-PCR/RNAseq, although any direct influence on intestinal transport has not been identified. We tested the hypothesis that cholinergic-induced anion secretion in the intestine is a result of both muscarinic and nicotinic pathways that are intrinsic to the intestinal epithelia. We dev...
Source: Am J Physiol Cell Ph... - June 17, 2020 Category: Cytology Authors: Johnson K, Yin J, In JG, Kulkarni S, Pasricha P, Tse M, Donowitz M Tags: Am J Physiol Cell Physiol Source Type: research

Circulating miRNAs - let's not waste the potential.
Abstract No abstract is required. PMID: 32551857 [PubMed - as supplied by publisher] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - June 17, 2020 Category: Cytology Authors: Hiam D, Lamon S Tags: Am J Physiol Cell Physiol Source Type: research

Coordinated CRYAB phosphorylation and desmin expression indicate adaptation and de-adaptation to resistance exercise-induced loading in human skeletal muscle.
In conclusion, pCRYABS59-responseindicates increase and decrease in resistance to CiL, in which a reinforced desmin network could play an essential role by structurally stabilizing the cells. PMID: 32520607 [PubMed - as supplied by publisher] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - June 10, 2020 Category: Cytology Authors: Jacko D, Bersiner K, Schulz O, Przyklenk A, Spahiu F, Höhfeld J, Bloch W, Gehlert S Tags: Am J Physiol Cell Physiol Source Type: research

Downregulation of the Ca2+-activated K+ channel KCa3.1 in mouse preosteoblast cells treated with vitamin D receptor agonist.
In this study, we found that the functional activity of intermediate-conductance Ca2+-activated K+ channels (KCa3.1) positively regulated cell proliferation in MC3T3-E1 cells derived from mouse preosteoblasts, by enhancing intracellular Ca2+ signaling. We examined the effects of treatment with vitamin D receptor (VDR) agonist on the expression and activity of KCa3.1 by real-time PCR examination, western blotting, Ca2+ imaging, and patch-clamp analyses in mouse MC3T3-E1 cells. Following the downregulation of KCa3.1 transcriptional modulators, such as Fra-1 and HDAC2, KCa3.1 activity was suppressed in MC3T3-E1 cells treated ...
Source: Am J Physiol Cell Ph... - June 10, 2020 Category: Cytology Authors: Kito H, Morihiro H, Sakakibara Y, Endo K, Kajikuri J, Suzuki T, Ohya S Tags: Am J Physiol Cell Physiol Source Type: research

ARF Family GTPases with Links to Cilia.
Abstract The ARF superfamily of regulatory GTPases, including both the ARF and ARL proteins, control a multitude of cellular functions including aspects of vesicular traffic, lipid metabolism, mitochondrial architecture, the assembly and dynamics of the microtubule and actin cytoskeletons, and other pathways in cell biology. Considering their general utility, it is perhaps not surprising that increasingly ARF/ARLs have been found in connection to primary cilia. Here, we critically evaluate the current knowledge of the role four ARF/ARLs (ARF4, ARL3, ARL13B and ARL6) play in cilia and highlight key missing informat...
Source: Am J Physiol Cell Ph... - June 10, 2020 Category: Cytology Authors: Fisher S, Kuna D, Caspary T, Kahn RA, Sztul E Tags: Am J Physiol Cell Physiol Source Type: research

The pH dependence of the Slc4a11-mediated H+ ‑conductance is influenced by intracellular lysine residues and modified by disease-linked mutations.
The pH dependence of the Slc4a11-mediated H+‑conductance is influenced by intracellular lysine residues and modified by disease-linked mutations. Am J Physiol Cell Physiol. 2020 Jun 10;: Authors: Quade BN, Marshall A, Parker MD Abstract SLC4A11 is the only member of the SLC4 family that transports protons rather than bicarbonate. SLC4A11 is expressed in corneal endothelial cells and its mutation causes corneal endothelial dystrophy, though the mechanism of pathogenesis is unknown. We previously demonstrated that the magnitude of the H+ conductance (Gm) mediated by SLC4A11 is increased by rises in in...
Source: Am J Physiol Cell Ph... - June 10, 2020 Category: Cytology Authors: Quade BN, Marshall A, Parker MD Tags: Am J Physiol Cell Physiol Source Type: research

H2S as a potential defence against COVID-19?
Abstract The outbreak of COVID-19 pneumonia caused by a new coronavirus (SARS-CoV-2) is posing a global health emergency and has led to more than 380,000 deaths worldwide. The cell entry of SARS-CoV-2 depends on two host proteins angiotensin-converting enzyme 2 (ACE2) and TMPRSS2. There is currently no vaccine available and also no effective drug for the treatment of COVID-19. Hydrogen sulfide (H2S) as a novel gasotransmitter has been shown to protect against lung damage via its anti-inflammation, anti-oxidative stress, anti-viral, pro-survival, and anti-aging effects. In light of the research advances on H2S sign...
Source: Am J Physiol Cell Ph... - June 9, 2020 Category: Cytology Authors: Yang G Tags: Am J Physiol Cell Physiol Source Type: research

Decoding SARS-CoV-2 Hijacking of Host Mitochondria in Pathogenesis of COVID-19.
Abstract Due to ongoing pandemic around the world, the mechanisms underlying the SARS-CoV-2 induced COVID-19 are subject to intense investigation. Based on available data for the SARS-CoV-1 virus, we suggest how CoV-2 localization of RNA transcripts in mitochondria hijacks the host cell's mitochondrial function to viral advantage. Besides viral RNA transcripts, RNA also localizes to mitochondria. SARS-CoV-2 may manipulate mitochondrial function indirectly, first by ACE2 regulation of mitochondrial function, and once it enters the host cell, ORFS such as ORF-9b can directly manipulate mitochondrial function to evad...
Source: Am J Physiol Cell Ph... - June 8, 2020 Category: Cytology Authors: Singh KK, Chaubey G, Chen JY, Suravajhala P Tags: Am J Physiol Cell Physiol Source Type: research

miR-135b-dependent down-regulation of S100B promotes neural stem cell differentiation in a hypoxia/ischemia-induced cerebral palsy rat model.
In this study, we identified a novel microRNA-135b (miR-135b) in CP, and investigated its functional role in mediating NSC differentiation. CP models were established in Wistar rats and validated with the Y-maze test. Gain- and loss-of-function experimentation was performed on CP rats. Then NSCs were isolated and the expression patterns of miR-135b and S100B were altered in NSCs. S100B exhibited high expression in hippocampus tissues of CP models, which was targeted by miR-135b. miR-135b elevation or S100B silencing resulted in promoted NSC differentiation, alleviated brain injury and inhibited NSC apoptosis in hippocampus...
Source: Am J Physiol Cell Ph... - June 3, 2020 Category: Cytology Authors: Wen L, Sun J, Chen X, Du R Tags: Am J Physiol Cell Physiol Source Type: research

A Tale of Two DUSP27s - Proposed Resolution for the Naming of Distinct Dual-Specificity Phosphatases.
Abstract There are currently two dual specificity phosphatases that are referred to in the literature as DUSP27, which has the potential to cause significant confusion as work on both genes is still very preliminary. It is the aim of this Viewpoint to clarify the difference between these two genes and bring attention to this nomenclature redundancy. PMID: 32491926 [PubMed - as supplied by publisher] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - June 3, 2020 Category: Cytology Authors: Cooper LM, Waddell DS Tags: Am J Physiol Cell Physiol Source Type: research

Overexpressed microRNA-539-5p inhibits inflammatory response of neurons to impede the progression of cerebral ischemic injury by histone deacetylase 1.
Abstract Several microRNAs (miRNAs or miRs) regulate cerebral ischemic injury outcomes; however, little is known about the role of miR-539-5p during cerebral ischemic injury or the post-ischemic state. Cerebral ischemic injury was modeled in vitro by exposing human cortical neurons to oxygen-glucose deprivation (OGD) and in vivo by occluding the middle cerebral artery (MCAO) in a rat model. The effects of miR-539-5p, histone deacetylase 1 (HDAC1), and early growth response 2 (EGR2) on cerebral ischemia were investigated using gain- and loss-of-function experiments. We identified changes in miR-539-5p, HDAC1, EGR2,...
Source: Am J Physiol Cell Ph... - June 3, 2020 Category: Cytology Authors: Xue H, Liu J, Shi L, Yang H Tags: Am J Physiol Cell Physiol Source Type: research

A brief history of organoids.
i VSW Abstract In vitro cell cultures are crucial research tools for modelling human development and diseases. While the conventional monolayer cell cultures have been widely used in the past, the lack of tissue architecture and complexity of such model fails to inform the true biological processes in vivo. Recent advances in the organoid technology have revolutionized the in vitro culture tools for biomedical research by creating powerful three-dimensional (3D) models to recapitulate the cellular heterogeneity, structure and functions of the primary tissues. Such organoid technology enables researchers to recreat...
Source: Am J Physiol Cell Ph... - May 27, 2020 Category: Cytology Authors: Corrò C, Novellasdemunt L, Li VSW Tags: Am J Physiol Cell Physiol Source Type: research

Glucose-induced duration-dependent genome-wide DNA methylation changes in human endothelial cells.
Abstract DNA methylation, a critical epigenetic mechanism, plays an important role in governing gene expressions during biological processes such as aging, which is well known to be accelerated in hyperglycemia (diabetes). In the present study, we investigated the effects of glucose on whole-genome DNA methylation in small (HRECs) and large (HUVECs) vessel endothelial cell (EC) lines exposed to basal or high glucose-containing media for variable lengths of time. Using the Infinium EPIC array, we obtained 773,133 CpG sites (probes) for analysis. Unsupervised clustering of the top 5% probes identified four distinct ...
Source: Am J Physiol Cell Ph... - May 27, 2020 Category: Cytology Authors: Aref-Eshghi E, Biswas S, Chen C, Sadikovic B, Chakrabarti S Tags: Am J Physiol Cell Physiol Source Type: research

Mechanisms of altered skeletal muscle action potentials in the R6/2 mouse model of Huntington's disease.
Abstract Huntington's disease (HD) patients suffer from progressive and debilitating motor dysfunction for which only palliative treatment is currently available. Previously, we discovered reduced skeletal muscle Cl- channel (ClC-1) and inwardly rectifying K+ channel (Kir) currents in R6/2 HD transgenic mice. To further investigate the role of ClC-1 and Kir currents in HD skeletal muscle pathology, we measured the effect of reduced ClC-1 and Kir currents on action potential (AP) repetitive firing in R6/2 mice using two-electrode current-clamp. We found that R6/2 APs had a significantly lower peak amplitude, depola...
Source: Am J Physiol Cell Ph... - May 20, 2020 Category: Cytology Authors: Miranda DR, Reed E, Jama A, Bottomley M, Ren H, Rich MM, Voss AA Tags: Am J Physiol Cell Physiol Source Type: research

Extracellular adenosine enhances pulmonary artery vasa vasorum endothelium cell barrier function via the Gi/ELMO1/Rac1/PKA-dependent signaling mechanisms.
In this study we investigated in more detail the mechanisms linking Gi activation to downstream barrier protection pathways. Using a small interference RNA (siRNA) technique and Transendothelial Electrical Resistance (TER) assay, we found that the adaptor protein, ELMO1, the tyrosine phosphatase Shp2, and atypical Gi- and Rac-mediated PKA activation are implicated in VVEC barrier enhancement. In contrast, the actin-interacting GTP-binding protein, girdin and the PAK1 downstream target, LIM kinase, are not involved in this response. In addition, adenosine-dependent cytoskeletal rearrangement involves activation of cofilin a...
Source: Am J Physiol Cell Ph... - May 20, 2020 Category: Cytology Authors: Verin AD, Batori R, Kovacs-Kasa A, Cherian-Shaw M, Kumar S, Czikora I, Karoor V, Strassheim D, Stenmark KR, Gerasimovskaya E Tags: Am J Physiol Cell Physiol Source Type: research

TNF α and IL-17 Alkalinize Airway Surface Liquid through CFTR and Pendrin.
TNFα and IL-17 Alkalinize Airway Surface Liquid through CFTR and Pendrin. Am J Physiol Cell Physiol. 2020 May 20;: Authors: Rehman T, Thornell IM, Pezzulo AA, Thurman AL, Romano Ibarra GS, Karp PH, Tan P, Duffey ME, Welsh MJ Abstract The pH of airway surface liquid (ASL) is a key factor that determines respiratory host defense; ASL acidification impairs and alkalinization enhances key defense mechanisms. Under healthy conditions, airway epithelia secrete base (HCO3¯) and acid (H+) to control ASL pH (pHASL). Neutrophil-predominant inflammation is a hallmark of several airway diseases, and TN...
Source: Am J Physiol Cell Ph... - May 20, 2020 Category: Cytology Authors: Rehman T, Thornell IM, Pezzulo AA, Thurman AL, Romano Ibarra GS, Karp PH, Tan P, Duffey ME, Welsh MJ Tags: Am J Physiol Cell Physiol Source Type: research

Advances in Aquaporin-2 trafficking mechanisms and their implications for treatment of water balance disorders.
Abstract In mammals, conservation of body water is critical for survival and is dependent on the kidneys ability to minimize water loss in the urine during periods of water deprivation. The collecting duct water channel aquaporin-2 (AQP2) plays an essential role in this homeostatic response by facilitating water reabsorption along osmotic gradients. The ability to increase the levels of AQP2 in the apical plasma membrane following an increase in plasma osmolality is a rate-limiting step in water reabsorption, a process that is tightly regulated by the antidiuretic hormone arginine vasopressin (AVP). In this review...
Source: Am J Physiol Cell Ph... - May 20, 2020 Category: Cytology Authors: Fenton RA, Murali SK, Moeller HB Tags: Am J Physiol Cell Physiol Source Type: research

RNA-binding protein HuR regulates translation of vitamin D receptor modulating rapid epithelial restitution after wounding.
Abstract Homeostasis of the intestinal epithelium is tightly regulated by numerous extracellular and intracellular factors including vitamin D and vitamin D receptor (VDR). VDR is highly expressed in the intestinal epithelium and is implicated in many aspects of gut mucosal pathophysiology, but the exact mechanism that controls VDR expression remains largely unknown. The RNA-binding protein HuR regulates the stability and translation of target mRNAs and thus modulates various cellular processes and functions. Here we report a novel role of HuR in the posttranscriptional control of VDR expression in the intestinal ...
Source: Am J Physiol Cell Ph... - May 20, 2020 Category: Cytology Authors: Zhang Y, Cai JZ, Xiao L, Chung HK, Ma XX, Chen LL, Rao JN, Wang JY Tags: Am J Physiol Cell Physiol Source Type: research

Cardiac Tissue Remodeling in Healthy Aging: The Road to Pathology.
Abstract This review aims to highlight the normal physiological remodeling that occurs in healthy aging hearts, including changes that occur in contractility, conduction, valve function, large and small coronary vessels and the extracellular matrix. These "normal" age-related changes serve as the foundation that supports decreased plasticity and limited ability for tissue remodeling during pathophysiological states, such as myocardial ischemia and heart failure. This review will identify populations at greater risk for poor tissue remodeling in advanced age along with current and future therapeutic strat...
Source: Am J Physiol Cell Ph... - May 20, 2020 Category: Cytology Authors: Tracy E, Rowe G, LeBlanc AJ Tags: Am J Physiol Cell Physiol Source Type: research

Recent advances in understanding cancer-associated fibroblasts in pancreatic cancer.
Abstract Pancreatic ductal adenocarcinoma (PDA) is a devastating disease with a poor survival rate. It is resistant to therapy in part due to its unique tumor microenvironment, characterized by a desmoplastic reaction resulting in a dense stroma that constitutes a large fraction of the tumor volume. A major contributor to the desmoplastic reaction is cancer-associated fibroblasts (CAFs). CAFs actively interact with cancer cells and promote tumor progression by different mechanisms, including extracellular matrix deposition, remodeling, and the secretion of tumor promoting factors, making CAFs an attractive target ...
Source: Am J Physiol Cell Ph... - May 20, 2020 Category: Cytology Authors: Huang H, Brekken RA Tags: Am J Physiol Cell Physiol Source Type: research

Functional Modulation of Sarcolemmal KATP Channels by Atrial Natriuretic Peptide-Elicited Intracellular Signaling in Adult Rabbit Ventricular Cardiomyocytes.
In conclusion, here we report that ANP positively modulates the activity of ventricular sarcKATP channels via an intracellular signaling mechanism consisting of NPR-A, PKG, ROS, ERK1/2, CaMKII and RyR2. This novel mechanism may regulate cardiac excitability and contribute to cytoprotection, in part, by opening myocardial KATP channels. PMID: 32432931 [PubMed - as supplied by publisher] (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - May 20, 2020 Category: Cytology Authors: Zhang DM, Lin YF Tags: Am J Physiol Cell Physiol Source Type: research

Thermal injury initiates pervasive fibrogenesis in skeletal muscle.
Abstract Severe burn injury induces a myriad of deleterious effects to skeletal muscle, resulting in impaired function and delayed recovery. Following burn, catabolic signaling and myofiber atrophy are key fiber-intrinsic determinants of weakness; less well understood are alterations in the interstitial environment surrounding myofibers. Muscle quality, specifically alterations in the extracellular matrix (ECM), modulates force transmission and strength. We sought to determine the impact of severe thermal injury on adaptation to the muscle ECM and quantify muscle fibrotic burden. After a 30% total body surface are...
Source: Am J Physiol Cell Ph... - May 20, 2020 Category: Cytology Authors: Brightwell CR, Hanson ME, El Ayadi A, Prasai A, Wang Y, Finnerty CC, Fry CS Tags: Am J Physiol Cell Physiol Source Type: research

Global Phosphotyrosinylated Protein Profile of Cell-Matrix Adhesion Complexes of Trabecular Meshwork Cells.
Abstract Dysregulation of the mechanical properties and cell adhesive interactions of trabecular meshwork (TM) are known to impair aqueous humor drainage and elevate intraocular pressure in glaucoma patients. The identity of regulatory mechanisms underlying TM mechanotransduction however, remains elusive. Here we analyzed the phosphotyrosine proteome of human TM cell-extracellular matrix (ECM) adhesion complexes which play a key role in sensing and transducing extracellular chemical and mechanical cues into intracellular activities, using a two-level affinity pull-down (phosphotyrosine antibody and titanium dioxid...
Source: Am J Physiol Cell Ph... - May 20, 2020 Category: Cytology Authors: Maddala R, Rao PV Tags: Am J Physiol Cell Physiol Source Type: research

Effect of Luminal Flow on Doming of mpkCCD Cells in a 3D Perfusable Kidney Cortical Collecting Duct Model.
in LM Abstract The cortical collecting duct (CCD) of the mammalian kidney plays a major role in the maintenance of total body electrolyte, acid/base and fluid homeostasis by tubular reabsorption and excretion. The mammalian CCD is heterogeneous, comprised of Na+-absorbing principal cells (PCs) and acid-base transporting intercalated cells (ICs). Perturbations in luminal flow rate alter hydrodynamic forces to which these cells in the cylindrical tubules are exposed. However, most studies of tubular ion transport have been performed in cell monolayers grown on or epithelial sheets affixed to a flat support, since an...
Source: Am J Physiol Cell Ph... - May 13, 2020 Category: Cytology Authors: Rein JL, Heja S, Flores D, Carrisoza-Gaytán R, Lin NYC, Homan KA, Lewis JA, Satlin LM Tags: Am J Physiol Cell Physiol Source Type: research

Divergent effects of genetic and pharmacological inhibition of Nox2 NADPH oxidase on insulin resistance related vascular damage.
Abstract Insulin resistance leads to excessive endothelial cell (EC) superoxide generation and accelerated atherosclerosis. The principal source of superoxide from the insulin-resistant endothelium is the Nox2 isoform of NADPH oxidase. Here we examine the therapeutic potential of Nox2 inhibition on: superoxide generation in saphenous vein EC (SVEC) from patients with advanced atherosclerosis and type 2 diabetes; and on vascular function, vascular damage and lipid deposition in Apolipoprotein E deficient (ApoE-/-) mice with EC specific insulin resistance (ESMIRO). To examine the effect of genetic inhibition of Nox2...
Source: Am J Physiol Cell Ph... - May 13, 2020 Category: Cytology Authors: Maqbool A, Watt N, Haywood N, Viswambharan H, Skromna A, Makava N, Visnagri A, Shawer HM, Bridge K, Muminov SK, Griffin K, Beech DJ, Wheatcroft SB, Porter KE, Simmons KJ, Sukumar P, Shah AM, Cubbon RM, Kearney MT, Yuldasheva NY Tags: Am J Physiol Cell Physiol Source Type: research

Am J Physiol Cell Physiol; +38 new citations
38 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Cell Physiol These pubmed results were generated on 2020/05/06PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - May 7, 2020 Category: Cytology Tags: Report Source Type: research

A peroxidase mimetic protects skeletal muscle cells from peroxide challenge and stimulates insulin signaling.
Abstract Reactive oxygen species such as hydrogen peroxide have been implicated in causing metabolic dysfunction such as insulin resistance. Heme groups, either by themselves or when incorporated into proteins have been shown to scavenge peroxide and demonstrate protective effects in various cell types. Thus, we hypothesized that a metalloporphyrin similar in structure to heme, Fe(III)tetrakis(4-benzoic acid)porphyrin (FeTBAP), would be a peroxidase mimetic that could defend cells against oxidative stress. After demonstrating that FeTBAP has peroxidase activity with reduced nicotinamide adenine dinucleotide phosph...
Source: Am J Physiol Cell Ph... - April 29, 2020 Category: Cytology Authors: Eccardt AM, Pelzel RJ, Mattathil L, Moon YA, Mannino MH, Janowiak BE, Fisher JS Tags: Am J Physiol Cell Physiol Source Type: research

Traction and attraction: Haptotaxis substrates collagen and fibronectin interact with chemotaxis by HGF to regulate myoblast migration in a microfluidic device.
Abstract Cell migration is central to development, wound healing, tissue regeneration, and immunity. Despite extensive knowledge of muscle regeneration, myoblast migration during regeneration is not well understood. C2C12 mouse myoblast migration and morphology were investigated using a triple-docking polydimethylsiloxane-based microfluidic device in which cells moved under gravity-driven laminar flow on uniform (=) collagen (CN=), fibronectin (FN=), or opposing gradients (CN-FN or FN-CN). In haptotaxis experiments, migration was faster on FN= than on CN=. At 10 hr, cells were more elongated on FN-CN and migration...
Source: Am J Physiol Cell Ph... - April 29, 2020 Category: Cytology Authors: Roveimiab Z, Lin F, Anderson JE Tags: Am J Physiol Cell Physiol Source Type: research

Cardiomyoblast caveolin expression: Effects of simulated diabetes, α-linolenic acid and cell signaling pathways.
Cardiomyoblast caveolin expression: Effects of simulated diabetes, α-linolenic acid and cell signaling pathways. Am J Physiol Cell Physiol. 2020 Apr 29;: Authors: Russell JS, Griffith TA, Peart JN, Headrick JP Abstract Caveolins regulate myocardial substrate handling, survival signaling and stress-resistance, however control of expression is incompletely defined. We test how metabolic features of type 2 diabetes (T2D), and modulation of cell signaling, influence caveolins in H9c2 cardiomyoblasts. Cells were exposed to glucose (25 vs. 5 mM), insulin (100 nM) or palmitate (0.1 mM), individually or...
Source: Am J Physiol Cell Ph... - April 29, 2020 Category: Cytology Authors: Russell JS, Griffith TA, Peart JN, Headrick JP Tags: Am J Physiol Cell Physiol Source Type: research

Ca2+ dependency of limb muscle fiber contractile mechanics in young and older adults.
Abstract Age-induced declines in skeletal muscle contractile function have been attributed to multiple cellular factors, including lower peak force (Po), decreased Ca2+ sensitivity, and reduced shortening velocity (Vo). However, changes in these cellular properties with aging remain unresolved, especially in older women, and the effect of submaximal Ca2+ on contractile function is unknown. Thus, we compared contractile properties of muscle fibers from 19 young (24±3 years; 8 women) and 21 older adults (77±7 years; 7 women) under maximal and submaximal Ca2+ and assessed the abundance of three proteins...
Source: Am J Physiol Cell Ph... - April 29, 2020 Category: Cytology Authors: Teigen LE, Sundberg CW, Kelly LJ, Hunter SK, Fitts RH Tags: Am J Physiol Cell Physiol Source Type: research