Substance P promotes TGF- β-induced collagen synthesis in human corneal fibroblasts
Am J Physiol Cell Physiol. 2024 Mar 25. doi: 10.1152/ajpcell.00084.2024. Online ahead of print.ABSTRACTCorneal fibroblasts maintain homeostasis of the corneal stroma by mediating the synthesis and degradation of extracellular collagen, and these actions are promoted by transforming growth factor-β (TGF-β) and interleukin-1b (IL-1β), respectively. The cornea is densely innervated with sensory nerve fibers that are not only responsible for sensation but also required for physiological processes such as tear secretion and wound healing. Loss or dysfunction of corneal nerves thus impairs corneal epithelial wound healing and...
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Koji Sugioka Teruo Nishida Junko Murakami Motoki Itahashi Mai Yunoki Shunji Kusaka Source Type: research

Advances in GPCRs: Structure, Mechanisms, Disease, and Pharmacology
Am J Physiol Cell Physiol. 2024 Mar 25. doi: 10.1152/ajpcell.00172.2024. Online ahead of print.NO ABSTRACTPMID:38525538 | DOI:10.1152/ajpcell.00172.2024 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Zeyu Cai Wei Kong Source Type: research

Regulation of renal aquaporin water channels in acute pyelonephritis
This study aimed to investigate if AQP2 expression in host cells increases UPEC infection efficiency and to identify specific bacterial components that mediate AQP2 plasma membrane insertion. Since the transepithelial water permeability in the collecting duct is co-determined by AQP3 and AQP4, we also investigated whether AQP3 and AQP4 localization is altered in the APN mouse model. We show that AQP2 expression does not increase UPEC infection efficiency and that AQP2 was targeted to the plasma membrane in AQP2 expressing cells in response to the two pathogen-associated molecular patterns (PAMPs), lipopolysaccharide and pe...
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Christina V Ernstsen Marianna Ranieri Fr édéric H Login Isra K Mahmoud Jacob R Therkildsen Giovanna Valenti Helle Praetorius Rikke N ørregaard Lene N Nejsum Source Type: research

The Role of Nano-systems in the Delivery of Glucose-lowering Drugs for the Pre-emption and Treatment of Diabetes-associated Atherosclerosis
Am J Physiol Cell Physiol. 2024 Mar 25. doi: 10.1152/ajpcell.00695.2023. Online ahead of print.ABSTRACTDiabetes is one of the most prevalent diseases worldwide. In recent decades, type-2 diabetes has become increasingly common, particularly in younger individuals. Diabetes leads to many vascular complications, including atherosclerosis. Atherosclerosis is a cardiovascular disease characterized by lipid-rich plaques within the vasculature. Plaques develop over time, restricting blood flow; and can therefore be the underlying cause of major adverse cardiovascular events, including myocardial infarction and stroke. Diabetes a...
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Luke James Conroy Alyssa McCann Nan Zhang Monica de Gaetano Source Type: research

Adipose transplantation improves metabolism and atherosclerosis but not PVAT abnormality or vascular dysfunction in lipodystrophic < em > Seipin/Apoe < /em > null mice
In conclusion, we demonstrate that adipose transplantation could alleviate lipodystrophy-associated metabolic disorders and atherosclerosis, but has an almost null impact on perivascular adipose abnormality or vascular dysfunction in lipodystrophic Seipin/Apoe double knockout mice.PMID:38525541 | DOI:10.1152/ajpcell.00698.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Zhe Meng Chuangxing Liu Mengke Xu Yongqiang Tao Haiyu Li Xijia Wang Jiawei Liao Mengyu Wang Source Type: research

Apelin regulates skeletal muscle adaptation to exercise in a high intensity interval training (HIIT) model
In conclusion, apelin is required for proper skeletal and cardiac muscle adaptation to high intensity exercise. Promoting apelinergic signaling may have benefits in aging- or disease-related muscle wasting conditions.PMID:38525542 | DOI:10.1152/ajpcell.00427.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Teemu Kilpi ö Sini Skarp Abel Perjes Julia Swan Leena Kaikkonen Samu Saarimaki Istvan Szokodi Josef M Penninger Zoltan Szabo Johanna Magga Risto Kerkela Source Type: research

Intracellular Zn < sup > 2+ < /sup > Promotes Extracellular Matrix Remodeling in Dexamethasone-Treated Trabecular Meshwork
Am J Physiol Cell Physiol. 2024 Mar 25. doi: 10.1152/ajpcell.00725.2023. Online ahead of print.ABSTRACTGiven the widespread application of glucocorticoids in ophthalmology, the associated elevation of intraocular pressure (IOP) has long been a vexing concern for clinicians, yet the underlying mechanisms remain inconclusive. Much of the discussion focuses on the extracellular matrix (ECM) of trabecular meshwork (TM). It's widely agreed that glucocorticoids impact the expression of Matrix metalloproteinases (MMPs), leading to ECM deposition. Since Zn2+ is vital for MMPs, we explored its role in ECM alterations induced by dex...
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Canying Liu Jiahui Tang Yuze Chen Qi Zhang Jicheng Lin Siting Wu Jiaxu Han Zhe Liu Caiqing Wu Yehong Zhuo Yiqing Li Source Type: research

Substance P promotes TGF- β-induced collagen synthesis in human corneal fibroblasts
Am J Physiol Cell Physiol. 2024 Mar 25. doi: 10.1152/ajpcell.00084.2024. Online ahead of print.ABSTRACTCorneal fibroblasts maintain homeostasis of the corneal stroma by mediating the synthesis and degradation of extracellular collagen, and these actions are promoted by transforming growth factor-β (TGF-β) and interleukin-1b (IL-1β), respectively. The cornea is densely innervated with sensory nerve fibers that are not only responsible for sensation but also required for physiological processes such as tear secretion and wound healing. Loss or dysfunction of corneal nerves thus impairs corneal epithelial wound healing and...
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Koji Sugioka Teruo Nishida Junko Murakami Motoki Itahashi Mai Yunoki Shunji Kusaka Source Type: research

Advances in GPCRs: Structure, Mechanisms, Disease, and Pharmacology
Am J Physiol Cell Physiol. 2024 Mar 25. doi: 10.1152/ajpcell.00172.2024. Online ahead of print.NO ABSTRACTPMID:38525538 | DOI:10.1152/ajpcell.00172.2024 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Zeyu Cai Wei Kong Source Type: research

Regulation of renal aquaporin water channels in acute pyelonephritis
This study aimed to investigate if AQP2 expression in host cells increases UPEC infection efficiency and to identify specific bacterial components that mediate AQP2 plasma membrane insertion. Since the transepithelial water permeability in the collecting duct is co-determined by AQP3 and AQP4, we also investigated whether AQP3 and AQP4 localization is altered in the APN mouse model. We show that AQP2 expression does not increase UPEC infection efficiency and that AQP2 was targeted to the plasma membrane in AQP2 expressing cells in response to the two pathogen-associated molecular patterns (PAMPs), lipopolysaccharide and pe...
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Christina V Ernstsen Marianna Ranieri Fr édéric H Login Isra K Mahmoud Jacob R Therkildsen Giovanna Valenti Helle Praetorius Rikke N ørregaard Lene N Nejsum Source Type: research

The Role of Nano-systems in the Delivery of Glucose-lowering Drugs for the Pre-emption and Treatment of Diabetes-associated Atherosclerosis
Am J Physiol Cell Physiol. 2024 Mar 25. doi: 10.1152/ajpcell.00695.2023. Online ahead of print.ABSTRACTDiabetes is one of the most prevalent diseases worldwide. In recent decades, type-2 diabetes has become increasingly common, particularly in younger individuals. Diabetes leads to many vascular complications, including atherosclerosis. Atherosclerosis is a cardiovascular disease characterized by lipid-rich plaques within the vasculature. Plaques develop over time, restricting blood flow; and can therefore be the underlying cause of major adverse cardiovascular events, including myocardial infarction and stroke. Diabetes a...
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Luke James Conroy Alyssa McCann Nan Zhang Monica de Gaetano Source Type: research

Adipose transplantation improves metabolism and atherosclerosis but not PVAT abnormality or vascular dysfunction in lipodystrophic < em > Seipin/Apoe < /em > null mice
In conclusion, we demonstrate that adipose transplantation could alleviate lipodystrophy-associated metabolic disorders and atherosclerosis, but has an almost null impact on perivascular adipose abnormality or vascular dysfunction in lipodystrophic Seipin/Apoe double knockout mice.PMID:38525541 | DOI:10.1152/ajpcell.00698.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Zhe Meng Chuangxing Liu Mengke Xu Yongqiang Tao Haiyu Li Xijia Wang Jiawei Liao Mengyu Wang Source Type: research

Apelin regulates skeletal muscle adaptation to exercise in a high intensity interval training (HIIT) model
In conclusion, apelin is required for proper skeletal and cardiac muscle adaptation to high intensity exercise. Promoting apelinergic signaling may have benefits in aging- or disease-related muscle wasting conditions.PMID:38525542 | DOI:10.1152/ajpcell.00427.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Teemu Kilpi ö Sini Skarp Abel Perjes Julia Swan Leena Kaikkonen Samu Saarimaki Istvan Szokodi Josef M Penninger Zoltan Szabo Johanna Magga Risto Kerkela Source Type: research

Intracellular Zn < sup > 2+ < /sup > Promotes Extracellular Matrix Remodeling in Dexamethasone-Treated Trabecular Meshwork
Am J Physiol Cell Physiol. 2024 Mar 25. doi: 10.1152/ajpcell.00725.2023. Online ahead of print.ABSTRACTGiven the widespread application of glucocorticoids in ophthalmology, the associated elevation of intraocular pressure (IOP) has long been a vexing concern for clinicians, yet the underlying mechanisms remain inconclusive. Much of the discussion focuses on the extracellular matrix (ECM) of trabecular meshwork (TM). It's widely agreed that glucocorticoids impact the expression of Matrix metalloproteinases (MMPs), leading to ECM deposition. Since Zn2+ is vital for MMPs, we explored its role in ECM alterations induced by dex...
Source: Am J Physiol Cell Ph... - March 25, 2024 Category: Cytology Authors: Canying Liu Jiahui Tang Yuze Chen Qi Zhang Jicheng Lin Siting Wu Jiaxu Han Zhe Liu Caiqing Wu Yehong Zhuo Yiqing Li Source Type: research

TIMP2 protects against sepsis-associated acute kidney injury by cAMP/NLRP3 axis-mediated pyroptosis
In this study, we investigated the impact of kidney tubule-specific Timp2 knockout mice on kidney injury and inflammation. Our findings demonstrated that Timp2-knockout mice exhibited more severe kidney injury than wild-type mice, along with elevated levels of pyroptosis markers NLRP3, Caspase1, and GSDMD in the early stage of SA-AKI. Conversely, the expression of exogenous TIMP2 in TIMP2-knockout mice still protected against kidney damage and inflammation. In vitro experiments, using recombinant TIMP2 protein, TIMP2 knockdown demonstrated that exogenous TIMP2 inhibited pyroptosis of renal tubular cells stimulated by LPS. ...
Source: Am J Physiol Cell Ph... - March 18, 2024 Category: Cytology Authors: Dongxue Xu Jun Jiang Ye Liu Jingjing Pang Jinmeng Suo Yiming Li Zhiyong Peng Source Type: research