Query: siRNA

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Total 14166 results found since Jan 2013.

Effect of CCL2 siRNA on proliferation and apoptosis in the U251 human glioma cell line.
In conclusion, CCL2 siRNA exhibited effective inhibition of cell proliferation and angiogenesis in the glioma cell line U251, which may provide a theoretical basis for the use of CCL2 in in vivo research and clinical treatment as a novel anticancer agent. PMID: 28714025 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - July 19, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Optimized solid-phase assisted synthesis of oleic acid-containing siRNA nanocarriers.
Abstract Cationic lipo-oligomers containing unsaturated oleic acid are potent siRNA carriers based on electrostatic and hydrophobic lipo-polyplex formation and endosomal membrane destabilization. Lipo-oligomers can be produced by solid-phase supported synthesis in sequence-defined form. However, the trifluoroacetic acid (TFA)-mediated removal of acid-labile protecting groups and cleavage from the resin can be accompanied by side products caused by addition of TFA to the double bonds of oleic acid. Under aqueous conditions, these TFA adducts of oleic acid are converted into hydroxystearic acid derivatives. The clea...
Source: ChemMedChem - July 17, 2017 Category: Chemistry Authors: Reinhard S, Zhang W, Wagner E Tags: ChemMedChem Source Type: research

Interfacial analysis of siRNA complexes with poly-ethylenimine (PEI) or PAMAM dendrimers in gene delivery
Publication date: 1 October 2017 Source:Colloids and Surfaces B: Biointerfaces, Volume 158 Author(s): Patrick K.C. Chang, Clive A. Prestidge, Kristen E. Bremmell Solution and interfacial analysis has been employed to gain insight into the complexation of siRNA using either G4 PAMAM dendrimers or 25kDa branched poly-ethylenimine (bPEI). The size, charge and shape/structure of the complexing agents were probed using atomic force microscopy (AFM), circular dichroism spectrometry (CD), dynamic light scattering (DLS), and gel electrophoresis (GE). The binding capability of these cationic polymers to the siRNA molecule, subsequ...
Source: Colloids and Surfaces B: Biointerfaces - July 16, 2017 Category: Biochemistry Source Type: research

Interleukin-4 receptor-targeted delivery of Bcl-xL siRNA sensitizes tumors to chemotherapy and inhibits tumor growth.
In this study, we exploited IL-4R-targeted delivery of Bcl-xL siRNA to IL-4R-expressing tumor cells in order to sensitize them to chemotherapy. To target IL-4R, an IL-4R-binding peptide, IL4RPep-1, was attached to branched polyethyleneimine-superparamagnetic iron oxide nanoparticles (BPEI-SPION). These nanoparticles were then complexed with Bcl-xL-targeting siRNA. IL-4R-targeted BPEI-SPION/Bcl-xL siRNA more efficiently reduced Bcl-xL gene expression and enhanced cytotoxicity of doxorubicin in MDA-MB231 breast tumor cells compared to untargeted BPEI-SPION/Bcl-xL siRNA. The siRNA was released from the complexes after 15 h o...
Source: Biomaterials - July 15, 2017 Category: Materials Science Authors: Guruprasath P, Kim J, Gunassekaran GR, Chi L, Kim S, Park RW, Kim SH, Baek MC, Bae SM, Kim SY, Kim DK, Park IK, Kim WJ, Lee B Tags: Biomaterials Source Type: research

Advances in quantum dot-mediated siRNA delivery
Publication date: Available online 13 July 2017 Source:Chinese Chemical Letters Author(s): Zhi-Yao He, Zhao-Hui Jin, Mei Zhan, Zhou Qin, Zhi Li, Ting Xu Nano-sized quantum dots (QDs) exhibit uniquely optical properties that are tunable with different sizes and shapes. QDs can emit narrow symmetric bands under a wide excitation range, possess anti-photobleaching stability, and be bio-functionalized on the large surface area. Therefore, QDs are attractive vectors for imaging-guided therapy. Small-interfering RNA (siRNAs)-based therapeutics hold great potential to target a large part of the currently undruggable genes, but o...
Source: Chinese Chemical Letters - July 14, 2017 Category: Chemistry Source Type: research

Amelioration of cirrhotic portal hypertension by targeted cyclooxygenase-1 siRNA delivery to liver sinusoidal endothelium with polyethylenimine grafted hyaluronic acid
In this study, hyaluronate-graft-polyethylenimine (HA-PEI) was synthesized for the targeted delivery of COX-1 siRNA to LSECs. Compared to non-targeted PEI, HA-PEI mediated much more efficient siRNA delivery, which resulted in potent targeted gene silencing in LSECs. In vivo, HA-PEI notably increased the accumulation of siRNA along the sinusoidal lining of the liver, inhibited over-activation of the COX-1/TXA2 pathway in LSECs, and successfully reduced portal pressure in cirrhotic mice. These results highlight the potential of HA-PEI complexed siRNA to serve as a LSECs-specific nanomedical system for effective gene therapy ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 13, 2017 Category: Nanotechnology Source Type: research

Bidirectional manipulation of gene expression in adipocytes using CRISPRa and siRNA
Conclusions We demonstrate that the CRISPRa SAM system can be easily adopted and used to efficiently manipulate gene expression in pre- and mature adipocytes in vitro. Moreover, we describe a novel methodological approach combining the activation of endogenous genes and siRNA-mediated gene silencing, thus providing a powerful tool to functionally decipher genetic factors controlling adipogenesis and adipocyte functions.
Source: Molecular Metabolism - July 9, 2017 Category: Endocrinology Source Type: research

Synthesis and characterization of amino acid-functionalized calcium phosphate nanoparticles for siRNA delivery
Publication date: 1 October 2017 Source:Colloids and Surfaces B: Biointerfaces, Volume 158 Author(s): Feray Bakan, Goknur Kara, Melike Cokol Cakmak, Murat Cokol, Emir Baki Denkbas Small interfering RNAs (siRNA) are short nucleic acid fragments of about 20–27 nucleotides, which can inhibit the expression of specific genes. siRNA based RNAi technology has emerged as a promising method for the treatment of a variety of diseases. However, a major limitation in the therapeutic use of siRNA is its rapid degradation in plasma and cellular cytoplasm, resulting in short half-life. In addition, as siRNA molecules cannot penetrate...
Source: Colloids and Surfaces B: Biointerfaces - July 8, 2017 Category: Biochemistry Source Type: research

A Combination of Guanidyl and Phenyl Groups on Dendrimer Enables Efficient siRNA and DNA Delivery.
This study provides a new strategy to develop multifunctional polymers for efficient siRNA and DNA delivery. PMID: 28686016 [PubMed - as supplied by publisher]
Source: Biomacromolecules - July 7, 2017 Category: Biochemistry Authors: Chang H, Zhang J, Wang H, Lv J, Cheng Y Tags: Biomacromolecules Source Type: research

Efficient dual siRNA and drug delivery using engineered lipoproteoplexes.
Abstract An engineered supercharged coiled-coil protein (CSP) and the cationic transfection reagent Lipofectamine 2000 are combined to form a lipoproteoplex for the purpose of dual delivery of siRNA and doxorubicin. CSP, bearing an external positive charge and axial hydrophobic pore, demonstrates the ability to condense siRNA and encapsulate the small molecule chemotherapeutic, doxorubicin. The lipoproteoplex demonstrates improved doxorubicin loading relative to Lipofectamine 2000. Furthermore, it induces effective transfection of GAPDH (60% knockdown) in MCF-7 breast cancer cells with efficiencies comparing favor...
Source: Biomacromolecules - July 7, 2017 Category: Biochemistry Authors: Liu CF, Chen R, Frezzo JA, Katyal P, Hill LK, Yin L, Srivastava N, More HT, Renfrew PD, Bonneau R, Montclare JK Tags: Biomacromolecules Source Type: research

Efficient receptor mediated siRNA delivery in vitro by folic acid targeted pentablock copolymer-based micelleplexes.
Abstract Novel, biocompatible polyplexes, based on the combination of cationic pentablock copolymers with folic acid functionalized copolymers were designed and developed for target-specific siRNA delivery. The resulting micelleplexes spontaneously formed polymeric micelles with a hydrophobic core surrounded directly by a cationic poly-2-(4-azidobutyl)-oxazole (PABOXA) and subsequently shielded by hydrophilic poly-2-methyl-oxazole (PMOXA) layer. The described micelleplexes form highly stable particles even in complete serum after 24 h compared with the highly cationic polymer PEI, which show aggregate formation in...
Source: Biomacromolecules - July 4, 2017 Category: Biochemistry Authors: Lehner R, Liu K, Wang X, Hunziker P Tags: Biomacromolecules Source Type: research

Mannose-conjugated layered double hydroxide nanocomposite for targeted siRNA delivery to enhance cancer therapy
Publication date: Available online 22 June 2017 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Li Li, Run Zhang, Wenyi Gu, Zhi Ping Xu Sheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moiety wa...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - July 1, 2017 Category: Nanotechnology Source Type: research

High-Content siRNA Screen of the Kinome Identifies Kinases Involved in Git2-Induced Mesenchymal-Epithelial Transition.
Abstract Epithelial-mesenchymal transition (EMT) and its reverse process mesenchymal-epithelial transition (MET) programs are involced in the metastatic process. More and more evidence confirms that EMT is vital for the initiation and dissemination of cancer cells whereas MET is critical for successful metastatic colonization of a secondary organ. The regulating mechanism of EMT mediated cancer progression and metastasis has been deeply investigated. However, what processes are dependent on MET in metastatic cascades remains unclear. Here, we created a cell based high-content siRNA screen using the breast cancer c...
Source: Molekuliarnaia Biologiia - July 1, 2017 Category: Molecular Biology Authors: Cao MG, Xu J, Yang QF, Guo ZP, Zhang KB, Li XB, Wu SQ, Zhou W Tags: Mol Biol (Mosk) Source Type: research

GSE100589 Gene expression profiling of SAS cell line transfected with siRNA targeting CYLD
Contributors : Satoru Shinriki ; Hirofumi JonoSeries Type : Expression profiling by arrayOrganism : Homo sapiensOral squamous cell carcinoma (OSCC) has a very poor prognosis due to its highly invasive nature, and 5-year survival rate has not changed apparently for the past 30 years. Although cylindromatosis (CYLD) is thought as a potent tumor suppressor, its biological and clinical significances in OSCC are largely unknown. The aim of this study was to clarify roles of CYLD in OSCC progression. Our immunohistochemical analyses revealed that CYLD expression was significantly reduced at invasive lesions in OSCC tissues where...
Source: GEO: Gene Expression Omnibus - June 29, 2017 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Co-delivery of paclitaxel and anti-survivin siRNA via redox-sensitive oligopeptide liposomes for the synergistic treatment of breast cancer and metastasis
Publication date: 30 August 2017 Source:International Journal of Pharmaceutics, Volume 529, Issues 1–2 Author(s): Xinyan Chen, Yidi Zhang, Chunming Tang, Chunli Tian, Qiong Sun, Zhigui Su, Lingjing Xue, Yifan Yin, Caoyun Ju, Can Zhang The overexpression of survivin in breast cancer cells is an important factor of paclitaxel (PTX) resistance in breast cancer. To overcome PTX resistance and improve the antitumor effect of PTX, we developed a novel liposome-based nanosystem (PTX/siRNA/SS-L), composed of a redox-sensitive cationic oligopeptide lipid (LHSSG2C14) with a proton sponge effect, natural soybean phosphatidylcholin...
Source: International Journal of Pharmaceutics - June 29, 2017 Category: Drugs & Pharmacology Source Type: research

Synthesis and characterization of amino acid-functionalized calcium phosphate nanoparticles for siRNA delivery.
Abstract Small interfering RNAs (siRNA) are short nucleic acid fragments of about 20-27 nucleotides, which can inhibit the expression of specific genes. siRNA based RNAi technology has emerged as a promising method for the treatment of a variety of diseases. However, a major limitation in the therapeutic use of siRNA is its rapid degradation in plasma and cellular cytoplasm, resulting in short half-life. In addition, as siRNA molecules cannot penetrate into the cell efficiently, it is required to use a carrier system for its delivery. In this work, chemically and morphologically different calcium phosphate (CaP) n...
Source: Colloids and Surfaces - June 27, 2017 Category: Biotechnology Authors: Bakan F, Kara G, Cokol Cakmak M, Cokol M, Denkbas EB Tags: Colloids Surf B Biointerfaces Source Type: research

Lipid nanoparticle delivery of glucagon receptor siRNA improves glucose homeostasis in mouse models of diabetes
Conclusion While leptin may be more effective than Gcgr siRNA at normalizing both glucose and lipid metabolism in STZ diabetes, Gcgr siRNA is more effective at reducing blood glucose levels in HFD/STZ diabetes.
Source: Molecular Metabolism - June 23, 2017 Category: Endocrinology Source Type: research

Mannose-conjugated layered double hydroxide nanocomposite for targeted siRNA delivery to enhanced cancer therapy
Publication date: Available online 22 June 2017 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Li Li, Run Zhang, Wenyi Gu, Zhi Ping Xu Sheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moiety wa...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - June 23, 2017 Category: Nanotechnology Source Type: research

Mannose-conjugated layered double hydroxide nanocomposite for targeted siRNA delivery to enhance cancer therapy
Sheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moiety was firstly conjugated onto SiO2-coated LDH nanocomposite.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - June 22, 2017 Category: Nanotechnology Authors: Li Li, Run Zhang, Wenyi Gu, Zhi Ping Xu Tags: Special Topic: Two-Dimensional Biomaterials in Regenerative Medicine Source Type: research

Mannose-conjugated layered double hydroxide nanocomposite for targeted siRNA delivery to enhanced cancer therapy
Sheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moiety was firstly conjugated onto SiO2-coated LDH nanocomposite.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - June 22, 2017 Category: Nanotechnology Authors: Li Li, Run Zhang, Wenyi Gu, Zhi Ping Xu Source Type: research

Mannose-conjugated layered double hydroxide nanocomposite for targeted siRNA delivery to enhance cancer therapy
Sheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moiety was firstly conjugated onto SiO2-coated LDH nanocomposite.
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - June 22, 2017 Category: Nanotechnology Authors: Li Li, Run Zhang, Wenyi Gu, Zhi Ping Xu Tags: Special Topic: Two-Dimensional Biomaterials in Regenerative Medicine Source Type: research

Polymer nanoassemblies with hydrophobic pendant groups in the core induce false positive siRNA transfection in luciferase reporter assays
This study elucidates a cause of the false positive effect in luciferase assays by using polymer nanoassemblies (PNAs) made from PEG, PEI, poly-(l-lysine) (PLL), palmitate (PAL), and deoxycholate (DOC): PEG-PEI (2P), PEG-PEI-PAL (3P), PEG-PLL (2P’), PEG-PLL-PAL (3P’), and PEG-PEI-DOC (2PD). In vitro transfection and western blot assays of luciferase using a colorectal cancer cell line expressing luciferase (HT29/LUC) concluded that 2P and 2P’ caused no luciferase expression reduction while hydrophobically modified PNAs induced a 35–50% reduction (3P’<2PD<3P). Although cell viability remained stagnan...
Source: International Journal of Pharmaceutics - June 21, 2017 Category: Drugs & Pharmacology Source Type: research

SiRNA ‐mediated down‐regulation of CLIC4 gene inhibits cell proliferation and accelerates cell apoptosis of mouse liver cancer Hca‐F and Hca‐P cells
This study explored the effects involved in silencing CLIC4 on apoptosis and proliferation of mouse liver cancer Hca‐F and Hca‐P cells. A CLIC4‐target small interfering RNA (siRNA) was designed to compound into two individual complementary oligonucleotide chains. A process of annealing and connection to a pSilencer vector was followed by transfection with Hca‐F and Hca‐P cells. Quantitative real‐time polymerase chain reaction and western blotting techniques were used to determine CLIC4 mRNA and protein expressions. CCK8 assay and flow cytometry were employed for analysis of the survival and apoptosis rate as we...
Source: Journal of Cellular Biochemistry - June 21, 2017 Category: Biochemistry Authors: Qiu ‐Yun Yu, Xin‐Feng Zhou, Qing Xia, Jia Shen, Jia Yan, Jiu‐Ting Zhu, Xiang Li, Ming Shu Tags: Article Source Type: research

Effective Skin Cancer Treatment by Topical Co-delivery of Curcumin and STAT3 siRNA Using Cationic Liposomes.
Abstract The aim of the present study was to evaluate the effectiveness of iontophoretic co-delivery of curcumin and anti-STAT3 siRNA using cationic liposomes against skin cancer. Curcumin was encapsulated in DOTAP-based cationic liposomes and then complexed with STAT3 siRNA. This nanocomplex was characterized for the average particle size, zeta-potential, and encapsulation efficiency. The cell viability studies in B16F10 mouse melanoma cells have shown that the co-delivery of curcumin and STAT3 siRNA significantly (p < 0.05) inhibited the cancer cell growth compared with either liposomal curcumin or STAT3 si...
Source: AAPS PharmSciTech - June 21, 2017 Category: Drugs & Pharmacology Authors: Jose A, Labala S, Ninave KM, Gade SK, Venuganti VVK Tags: AAPS PharmSciTech Source Type: research

Splicing stimulates siRNA formation at < i > Drosophila < /i > DNA double-strand breaks
by Karin Merk, Marco Breinig, Romy B öttcher, Stefan Krebs, Helmut Blum, Michael Boutros, Klaus Förstemann DNA double-strand breaks trigger the production of locus-derived siRNAs in fruit flies, human cells and plants. At least in flies, their biogenesis depends on active transcription running towards the break. Since siRNAs derive from a double-stranded RNA precursor, a major question is how broken DN A ends can generate matching sense and antisense transcripts. We performed a genome-wide RNAi-screen in culturedDrosophila cells, which revealed that in addition to DNA repair factors, many spliceosome components are requ...
Source: PLoS Genetics - June 19, 2017 Category: Genetics & Stem Cells Authors: Karin Merk Source Type: research

Genes, Vol. 8, Pages 163: Using Small RNA-seq Data to Detect siRNA Duplexes Induced by Plant Viruses
In this study, for the first time, we detected siRNA duplexes induced by plant viruses on a large scale using next-generation sequencing (NGS) data . In addition, we used the detected 21 nucleotide (nt) siRNA duplexes with 2 nt overhangs to construct a dataset for future data mining. The analytical results of the features in the detected siRNA duplexes were consistent with those from previous studies. The investigation of siRNA duplexes is usef ul for a better understanding of the RNA interference (RNAi) mechanism. It can also help to improve the virus detection based on the small RNA sequencing (sRNA-seq) technologies and...
Source: Genes - June 16, 2017 Category: Genetics & Stem Cells Authors: Xiaoran Niu Yu Sun Ze Chen Rugang Li Chellappan Padmanabhan Jishou Ruan Jan Kreuze KaiShu Ling ZhangJun Fei Shan Gao Tags: Communication Source Type: research

Recent advances in mechanism-based chemotherapy drug-siRNA pairs in co-delivery systems for cancer: A review
Publication date: 1 September 2017 Source:Colloids and Surfaces B: Biointerfaces, Volume 157 Author(s): Mingfang Wang, Jinyu Wang, Bingcheng Li, Lingxin Meng, Zhaoxing Tian Co-delivery of chemotherapy drugs and siRNA for cancer therapy has achieved remarkable results according to synergistic/combined antitumor effects, and is recognized as a promising therapeutic modality. However, little attention has been paid to the extremely complex mechanisms of chemotherapy drug-siRNA pairs during co-delivery process. Proper selection of chemotherapy drug-siRNA pairs is beneficial for achieving desirable cancer therapeutic effects. ...
Source: Colloids and Surfaces B: Biointerfaces - June 12, 2017 Category: Biochemistry Source Type: research

Recent advances in mechanism-based chemotherapy drug-siRNA pairs in co-delivery systems for cancer: A review.
Abstract Co-delivery of chemotherapy drugs and siRNA for cancer therapy has achieved remarkable results according to synergistic/combined antitumor effects, and is recognized as a promising therapeutic modality. However, little attention has been paid to the extremely complex mechanisms of chemotherapy drug-siRNA pairs during co-delivery process. Proper selection of chemotherapy drug-siRNA pairs is beneficial for achieving desirable cancer therapeutic effects. Exploring the inherent principles during chemotherapy drug-siRNA pair selection for co-delivery would greatly enhanced therapeutic efficiency. To achieve id...
Source: Colloids and Surfaces - June 8, 2017 Category: Biotechnology Authors: Wang M, Wang J, Li B, Meng L, Tian Z Tags: Colloids Surf B Biointerfaces Source Type: research

Controlled and sustained delivery of siRNA/NPs from hydrogels expedites bone fracture healing.
Abstract Despite great potential, delivery remains as the most significant barrier to the widespread use of siRNA therapeutics. siRNA has delivery limitations due to susceptibility to RNase degradation, low cellular uptake, and poor tissue-specific localization. Here, we report the development of a hybrid nanoparticle (NP)/hydrogel system that overcomes these challenges. Hydrogels provide localized and sustained delivery via controlled release of entrapped siRNA/NP complexes while NPs protect and enable efficient cytosolic accumulation of siRNA. To demonstrate therapeutic efficacy, regenerative siRNA against WW do...
Source: Biomaterials - June 4, 2017 Category: Materials Science Authors: Wang Y, Malcolm DW, Benoit DSW Tags: Biomaterials Source Type: research

Silencing of ATM expression by siRNA technique contributes to glioma stem cell radiosensitivity in vitro and in vivo.
In conclusion, silencing of ATM via the siRNA technique improved radiosensitivity of GSCs both in vitro and in vivo. PMID: 28560406 [PubMed - as supplied by publisher]
Source: Oncology Reports - June 2, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

siRNA delivery system based on magnetic nanovectors: Characterization and stability evaluation.
Abstract Gene therapy and particularly small interfering RNA (siRNA) is a promising therapeutic method for treatment of various human diseases, especially cancer. However the lack of an ideal delivery system limits its clinical applications. Effective anticancer drug development represents the key for translation of research advances into medicines. Previously we reported, the optimization of magnetic siRNA nanovectors (MSN) formulation based on superparamagnetic iron oxide nanoparticles (SPION) and chitosan for systemic administration. This work aimed at using rational design to further optimize and develop MSN. ...
Source: European Journal of Pharmaceutical Sciences - June 1, 2017 Category: Drugs & Pharmacology Authors: Abdelrahman M, Eyrolles LD, Alkarib SY, Hervé-Aubert K, Djemaa SB, Marchais H, Chourpa I, David S Tags: Eur J Pharm Sci Source Type: research

TPP-dendrimer nanocarriers for siRNA delivery to the pulmonary epithelium and their dry powder and metered-dose inhaler formulations
Publication date: 15 July 2017 Source:International Journal of Pharmaceutics, Volume 527, Issues 1–2 Author(s): Elizabeth Bielski, Qian Zhong, Hamad Mirza, Matthew Brown, Ashura Molla, Teresa Carvajal, Sandro R.P. da Rocha The regulation of genes utilizing the RNA interference (RNAi) mechanism via the delivery of synthetic siRNA has great potential in the treatment of a variety of lung diseases. However, the delivery of siRNA to the lungs is challenging due to the poor bioavailability of siRNA when delivered intraveneously, and difficulty in formulating and maintaining the activity of free siRNA when delivered directly ...
Source: International Journal of Pharmaceutics - June 1, 2017 Category: Drugs & Pharmacology Source Type: research

Preclinical Mammalian Safety Studies of EPHARNA (DOPC Nanoliposomal EphA2-Targeted siRNA)
To address the need for efficient and biocompatible delivery systems for systemic siRNA delivery, we developed 1,2-Dioleoyl-sn-Glycero-3-Phosphatidylcholine (DOPC) nanoliposomal EphA2-targeted therapeutic (EPHARNA). Here, we performed safety studies of EPHARNA in murine and primate models. Single dosing of EPHARNA was tested at 5 concentrations in mice (N = 15 per group) and groups were sacrificed on days 1, 14, and 28 for evaluation of clinical pathology and organ toxicity. Multiple dosing of EPHARNA was tested in mice and Rhesus macaques twice weekly at two dose levels in each model. Possible effects on hematologic param...
Source: Molecular Cancer Therapeutics - June 1, 2017 Category: Cancer & Oncology Authors: Wagner, M. J., Mitra, R., McArthur, M. J., Baze, W., Barnhart, K., Wu, S. Y., Rodriguez-Aguayo, C., Zhang, X., Coleman, R. L., Lopez-Berestein, G., Sood, A. K. Tags: Large Molecule Therapeutics Source Type: research

Enhanced delivery of biodegradable mPEG-PLGA-PLL nanoparticles loading Cy3-labelled PDGF-BB siRNA by UTMD to rat retina.
Abstract We investigated the efficacy and safety of ultrasound (US)-targeted microbubble (MB) destruction (UTMD)-enhanced delivery of monomethoxypoly(ethylene glycol)-poly(lactic-co-glycolic acid)-poly-L-lysine (mPEG-PLGA-PLL) nanoparticles (NPs) loading Cy3-labelled platelet-derived growth factor BB (PDGF-BB) siRNA to rat retina in vivo. Eighty Wistar rats were divided into five groups (G). The right eyes, respectively, received an intravitreal injection as follows: normal saline (NS) (G1), NPs and NS (G2), NPs and MBs (G3), NPs and NS (G4) and NPs and MBs (G5). In G4 and G5, the eyes were exposed to US for 5 min...
Source: Journal of Biosciences - June 1, 2017 Category: Biomedical Science Authors: DU J, Sun Y, Li FH, DU LF, Duan YR Tags: J Biosci Source Type: research

Study of the role of siRNA mediated promoter methylation in DNMT3B knockdown and alteration of ...
Promoter methylation is one of the main epigenetic mechanisms that lead to the inactivation of tumor suppressor genes during carcinogenesis. Due to the reversible nature of DNA methylation, many studies have been performed to correct theses epigenetic defects by inhibiting DNA methyltransferases (DNMTs). In this case novel therapeutics especially siRNA oligonucleotides have been used to specifically knockdown the DNMTs at mRNA level. Also many studies have focused on transcriptional gene silencing in mammalian cells via siRNA mediated promoter methylation. The present study was designed to assess the role of siRNA mediated...
Source: Iranian Journal of Pharmaceutical Research - May 31, 2017 Category: Drugs & Pharmacology Source Type: research

Hollow carbon nanospheres as a versatile platform for co-delivery of siRNA and chemotherapeutics
Publication date: September 2017 Source:Carbon, Volume 121 Author(s): Lingmin Zhang, Xinglong Yang, Ying Li, Wenfu Zheng, Xingyu Jiang The synergistic treatment with therapeutic nucleic acids and chemotherapeutics is considered to be a feasible strategy to overcome drug-resistant cancers. Herein, we constructed a novel amine dotted hollow carbon nanospheres (HCNs) to serve as a versatile platform for co-delivery of siRNA targeting multidrug resistance gene (MDR1) mRNA (siMDR1) and chemotherapeutics (Doxorubicin or Cisplatin) to fight drug-resistant cancers. The HCNs show enhanced loading capability of both siRNA and chemo...
Source: Carbon - May 27, 2017 Category: Materials Science Source Type: research

ESM-1 siRNA Knockdown Decreased Migration and Expression of CXCL3 in Prostate Cancer Cells.
We report here for the first time the ESM-1 targeting in PC-3 cells, which resulted in decreased migration, which may be related, at least in part, to decreased expression of the angiogenic CXCL3 chemokine, whose expression was found to be reduced in ESM-1-siRNA transfected cells. Additional studies are required to ascertain the biological role of ESM-1 in prostate cancer cells and the link with the expression of CXCL3. PMID: 28533735 [PubMed - in process]
Source: International Journal of Biomedical Science - May 25, 2017 Category: Biomedical Science Tags: Int J Biomed Sci Source Type: research

siRNA delivery using polyelectrolyte-gold nanoassemblies in neuronal cells for BACE1 gene silencing
Publication date: 1 November 2017 Source:Materials Science and Engineering: C, Volume 80 Author(s): Aparna Chaudhary, Sanjeev Garg Small interfering RNA (siRNA) mediated RNA interference is a versatile therapeutic tool for many intractable genetic disorders. Various nanoassemblies specifically designed to deliver the siRNAs could be utilized for efficient siRNA delivery which is one of the major concern for the success of this therapeutic. Thus, in the present study, polyelectrolyte-gold nanoassemblies (PE-Gold NAs) were selected for siRNA delivery of an in vitro verified siRNA. Three different polyelectrolytes (polyethyl...
Source: Materials Science and Engineering: C - May 25, 2017 Category: Materials Science Source Type: research

siRNA-mediated knockdown of ID1 disrupts Nanog- and Oct-4-mediated cancer stem cell-likeness and resistance to chemotherapy in gastric cancer cells.
In conclusion, knockdown of ID1 attenuates the stem cell like-properties of self-renewal in normal GC cells, potentially through the targeting of Nanog and Oct-4, and subsequently decreases cell proliferation and resistance to DDP. The results of the present study suggest that ID1 functions as an oncogene in GC and regulates the stem cell like-properties of gastric cancer cells by targeting Nanog and Oct-4. PMID: 28529558 [PubMed - in process]
Source: Oncology Letters - May 24, 2017 Category: Cancer & Oncology Tags: Oncol Lett Source Type: research

A Poly(Propyleneimine) Dendrimer ‐Based Polyplex‐System for Single‐Chain Antibody‐Mediated Targeted Delivery and Cellular Uptake of SiRNA
This study reports a new modular polyplex carrier system for targeted delivery of siRNA, which is based on transfection‐disabled maltose‐modified poly(propyleneimine)‐dendrimers (mal‐PPI) bioconjugated to single chain fragment variables (scFvs). To achieve targeted delivery into tumor cells expressing the epidermal growth factor receptor variant III (EGFRvIII), monobiotinylated anti‐EGFRvIII scFv fused to a Propionibacterium shermanii transcarboxylase‐derived biotinylation acceptor (P‐BAP) is bioconjugated to mal‐PPI through a novel coupling strategy solely based on biotin–neutravidin bridging. In contras...
Source: Small - May 22, 2017 Category: Nanotechnology Authors: Stefanie Tietze, Isabell Schau, Susanne Michen, Franka Ennen, Andreas Janke, Gabriele Schackert, Achim Aigner, Dietmar Appelhans, Achim Temme Tags: Full Paper Source Type: research

siRNA-knockdown of ADAMTS-13 modulates endothelial cell angiogenesis
Publication date: Available online 22 May 2017 Source:Microvascular Research Author(s): Huiyuan Tang, Manfai Lee, Eun Ho Kim, Daniel Bishop, George M. Rodgers ADAMTS-13, a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13, is a zinc-containing metalloprotease that cleaves von Willebrand factor (vWf). Previous publications by our laboratory have shown that ADAMTS-13 may also be involved in angiogenesis. For this study, we report the successful transient knockdown of endogenous ADAMTS-13 in human umbilical vein endothelial cells (HUVEC) via siRNA and the effects of reduced endogenous ADAMTS-13 ...
Source: Microvascular Research - May 22, 2017 Category: Biochemistry Source Type: research

Charge reversible calcium phosphate lipid hybridnanoparticle for siRNA delivery.
Authors: Cai RQ, Liu DZ, Cui H, Cheng Y, Liu M, Zhang BL, Mei QB, Zhou SY Abstract Bcl-2 gene is an important target to treat lung cancer. The small interference RNA (siRNA) of Bcl-2 gene (siBcl-2) can specifically silence Bcl-2 gene. However, naked siBcl-2 is difficult to accumulate in the tumor tissue to exert its activity. In this paper, a calcium phosphate lipid hybrid nanoparticle that possessed charge reversible property was prepared to enhance the activity of siBcl-2 in vivo. The average diameter and zeta potential of siBcl-2 loaded calcium phosphate lipid hybrid nanoparticles (LNPS@siBcl-2) were 80 nm and -...
Source: Oncotarget - May 19, 2017 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

siRNA-mediated inactivation of HER3 improves the antitumour activity and sensitivity of gefitinib in gastric cancer cells.
Authors: Yuan HH, Yang YN, Zhou JH, Li YJ, Wang LY, Qin JW, Liu T, Li ZZ, Zhou QX, Wei XL, Zhang TT, Huang P, Zhang WJ, Liu L, Du XX, Han Y Abstract The human EGFR family consists of four type-1 transmembrane tyrosine kinase receptors: HER1 (EGFR, ErbB1), HER2 (Neu, ErbB2), HER3 (ErbB3), and HER4 (ErbB4). HER3 can dimerize with EGFR, HER2 and even c-Met and likely plays a central role in the response to EGFR-targeted therapy. Because HER3 lacks significant kinase activity and cannot be inhibited by tyrosine kinase inhibitors, neutralizing antibodies and alternative inhibitors of HER3 have been sought as cancer ther...
Source: Oncotarget - May 19, 2017 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Apoptosis induction activity and molecular docking studies of survivin siRNA carried by Fe3O4-PEG-LAC-chitosan-PEI nanoparticles in MCF-7 human breast cancer cells
Publication date: 5 August 2017 Source:Journal of Pharmaceutical and Biomedical Analysis, Volume 142 Author(s): Sanam Arami, Majid Mahdavi, Mohammad-Reza Rashidi, Reza Yekta, Mohammad Rahnamay, Leila Molavi, Mohammad-Saeid Hejazi, Nasser Samadi Delivery of small interfering RNAs (siRNAs) into cells still remains a challenge in gene delivery studies. Here, we investigated the ability of synthesized Fe3O4-PEG-LAC-chitosan-PEI nanoparticles for siRNA delivery of survivin as the model gene into cells. The cellular uptake of survivin siRNA carried by synthesized nanoparticles into MCF-7 breast cancer cell line was evaluated by...
Source: Journal of Pharmaceutical and Biomedical Analysis - May 13, 2017 Category: Drugs & Pharmacology Source Type: research

siRNA Targeting Net1 Reduces the Angiogenesis and Tumor Growth of in vivo Cervical Squamous Cell Carcinoma through VEGF down-regulation
Net1, a guanine nucleotide exchange factor, is implicated in cancer cell invasion through activation of RhoA. However, there is still no report on the association between Net1 and cancer angiogenesis. The current study was designed to explore the roles of Net1 in the angiogenesis of cervical squamous cell carcinoma (CSCC) and further observe the effects of Net1 siRNA on the tumor growth. Net1 was overexpressed in CSCC samples (n=80), correlated to the cancer microvessel density (MVD, r=0.223, P=.026) and related to aggressive clinical behaviors, including depth of cervical wall invasion (P=.041), parametrial involvement (P...
Source: Human Pathology - May 13, 2017 Category: Pathology Authors: Yuting Zhang, Pei Xia, Wenhui Zhang, Min Yan, Xiujuan Xiong, Weiwei Yu, Enlin Song Tags: Original contribution Source Type: research

Local Delivery of CTGF siRNA with Poly(sorbitol-co-PEI) Reduces Scar Contraction in Cutaneous Wound Healing
This study exemplifies a successful treatment of cutaneous wound healing using a polymer system coupled with RNA interference. Hence, the approach holds a great promise for developing new treatments with novel targets in regenerative medicines.
Source: Tissue Engineering and Regenerative Medicine - May 12, 2017 Category: Biotechnology Source Type: research

siRNA-nanoparticle conjugate in gene silencing: A future cure to deadly diseases?
Abstract Alzheimers, cancer, acquired immune deficiency syndrome (AIDS) are considered to be some of the most deadly diseases of the 21st century on account of their severity and rapid increase in the number of affected population and with scarce cases of recovery, they still remain a troubling paradox. Specifically, with millions of cancer patients worldwide and lack of proper cure for the same, understanding the deadly disease at the molecular level and planning a therapeutic strategy in the same line is the need of the hour. Further, the potential threat of prevalence and escalation of Alzheimer's and HIV (huma...
Source: Appl Human Sci - May 11, 2017 Category: Physiology Authors: Acharya R, Saha S, Ray S, Hazra S, Mitra MK, Chakraborty J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

Delivering siRNA with Dendrimers: In Vivo Applications.
Abstract Over the last decades, gene therapy has emerged as a pioneering therapeutic approach to treat or prevent several diseases. Among the explored strategies, the short-term silencing of protein coding genes mediated by siRNAs has a good therapeutic potential in a clinical setting. However, the widespread use of siRNA will require the development of clinically suitable, safe and effective vehicles with the ability to complex and deliver siRNA into target cells with minimal toxicity. Lately, dendrimers have gained considerable attention as non-viral vectors in nucleic acid delivery due to their unique structura...
Source: Current Gene Therapy - May 10, 2017 Category: Genetics & Stem Cells Authors: Leiro V, Santos SD, Pêgo AP Tags: Curr Gene Ther Source Type: research

Study of the role of siRNA mediated promoter methylation in DNMT3B knockdown and alteration of ...
Promoter methylation is one of the main epigenetic mechanisms that lead to the inactivation of tumor suppressor genes during carcinogenesis. Due to the reversible nature of DNA methylation, many studies have been performed to correct theses epigenetic defects by inhibiting DNA methyltransferases (DNMTs). In this case novel therapeutics especially siRNA oligonucleotides have been used to specifically knockdown the DNMTs at mRNA level. Also many studies have focused on transcriptional gene silencing in mammalian cells via siRNA mediated promoter methylation. The present study was designed to assess the role of siRNA mediated...
Source: Iranian Journal of Pharmaceutical Research - May 9, 2017 Category: Drugs & Pharmacology Source Type: research